CN1923996A - Apirulina containing spirulina bioactive peptide and spirulina proteinase, preparation method and application thereof - Google Patents
Apirulina containing spirulina bioactive peptide and spirulina proteinase, preparation method and application thereof Download PDFInfo
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- CN1923996A CN1923996A CN 200610159691 CN200610159691A CN1923996A CN 1923996 A CN1923996 A CN 1923996A CN 200610159691 CN200610159691 CN 200610159691 CN 200610159691 A CN200610159691 A CN 200610159691A CN 1923996 A CN1923996 A CN 1923996A
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- spirulina
- bioactive peptide
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- protein enzyme
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Abstract
The invention discloses a spirulina with active spirulina peptide and spirulina prolease and preparing method and application, which is characterized by the following: adjusting human blood fat; adopting bacillus subtilis to ferment spirulina; making the active spirulina peptide at 15-40% when the water content is under 7%; possessing 1000-40000U/g spirulina prolease.
Description
Technical field
What the present invention relates to is a kind of spirulina, particularly a kind of spirulina that contains spirulina bioactive peptide, spirulina protein enzyme, and this spirulina bioactive peptide, spirulina protein enzyme exist with molecular form; The invention still further relates to the preparation method of this spirulina and the application of this spirulina.
Background technology
Spirulina contains multiple amino acids, also contain multivitamin, carotene, unsaturated fatty acids and to human body beneficial's polysaccharide, can provide the multiple nutrients composition to human body, regulate the physiological function of human body, strengthening immunity is a kind of high nutrition, whole food low in calories, for the irrational meals trencherman of trophic structure, be undoubtedly optimal health-care good product, the disease for lacking due to the nutrient substance can play extra-nutrition to reach the effect that health care gets rid of illness.At present, China hygiene control department has been decided to be spirulina the protective foods of adjusting blood fat, yet as protective foods, it acts on mitigation, and curative effect is slow, thereby has influenced the popularization and the application of spirulina.Therefore, at present, be to be placed on to improve spirulina curative effect aspect to the main direction of the very big some of research of spirulina.The effect of the adjustment human body blood fat that spirulina had, mainly be because it contains carotene, chlorophyll, superoxide dismutase and calcium, magnesium, selenium and other trace elements, the comprehensive action of these compositions just makes spirulina have the effect of adjusting blood fat, but the effect of this respect is still relatively milder with slowly.In addition, from present research, also do not find to contain in the spirulina spirulina bioactive peptide, the spirulina protein enzyme that exist with molecular form, it is known containing protein and amino acid in the spirulina, yet we know, peptide different proteins and amino acid, peptide are the compounds of molecular structure between amino acid and protein.The molecular structure complexity of most protein, relative molecular weight are more than 100,000, and numberator height compression, folding, have formed three-dimensional regular entity, and these complicated structures have a strong impact on body to proteinic digestion and absorption just.But the molecular weight ratio protein of peptide is little a lot, thereby has the unexistent physiological regulation function of some protein.On the other hand, peptide is different from amino acid, and this aspect such as physiological activity, body absorpting form, body absorption characteristics that shows peptide is all inequality with amino acid.Process biological method from spirulina, prepare the spirulina that contains spirulina bioactive peptide and coilin enzyme, thereby developing its physiological active functions, particularly develop its direct thrombus and thrombus and a series of physiological activities of bringing, is highly significant.And according to retrieval, and find no the Bi Lu of this respect technology.
Summary of the invention
Purpose of the present invention provides a kind of spirulina that contains spirulina bioactive peptide, spirulina protein enzyme, makes it have specific effect, to satisfy the needs of some specific health-care effect.
Another object of the present invention provides the preparation method of this spirulina.
Another purpose of the present invention provides the purposes of this spirulina.
The objective of the invention is to realize: the spirulina that contains spirulina bioactive peptide, spirulina protein enzyme by following measure, mainly contain following composition: protein, amino acid, carbohydrate, fat, protein, robust fibre, vitamins C, vitamins B, vitamin-E, class Radix Dauci Sativae, inositol, chlorophyll, linolenic acid, superoxide dismutase, germanium, magnesium, calcium, iron, phosphorus, potassium, zinc, selenium, polysaccharide and moisture, when described spirulina water content is 7% also to contain the spirulina bioactive peptide of 15-40% and the spirulina protein enzyme of 1000-40000U/g when following.
The above-mentioned spirulina that contains spirulina bioactive peptide, spirulina protein enzyme mainly is prepared from by following method:
(1) (B acillus subtilis subsp subtilis bacterium numbering is: 1.1086 with subtilis, the used bacterial classification of the present invention is preserved by Institute of Microorganism, Academia Sinica) be inoculated on the substratum, seven substratum of every bacterial classification inoculation, substratum is a nutrition nutrient agar substratum, constant volume is 1000 milliliters, cultivates 50-65 hour under 26-35 ℃ of condition;
(2) getting the weight ratio that above-mentioned cultured bacteria culture fluid 3-5 milliliter adds spirulina powder and water is 1: stir in the 30-50 kilogram spirulina slurry of 1.2-1.4,36-40 ℃ condition bottom fermentation 12-18 hour, during regularly stir and allow the algae slurry evenly ferment;
(3) spirulina that ferments is starched under the condition that places 2-8 ℃ and was stablized after-ripening 60-120 hour, also can under this condition, preserve, and be to get final product below 7% again in the temperature drying below 45 ℃ to water content;
(4) above-mentioned dry spirulina is then obtained spirulina capsule with encapsulated; Above-mentioned dry spirulina is added that the appropriate amount of auxiliary materials compacting then obtains spirulina tablet in flakes.
The invention described above has thrombolytic effect, and high blood viscosity is had preventive and therapeutic effect, is applied to the control of human body essential hypertension.
Take the present invention of above-mentioned measure, introduce subtilis spirulina is fermented, thereby make spirulina produce biological variation, generated elastic helix algae bioactive peptide and spirulina protein enzyme, produced a series of active function therefrom.After testing, when water content of the present invention is 7% when following, the content of spirulina bioactive peptide is that 15-40%, spirulina protein enzyme are 1000-40000U/g among the present invention.Spirulina bioactive peptide of the present invention, spirulina protein enzyme iso-electric point are about 8.1, and relative molecular weight detects through hemolytic test between 1000-25000, and the present invention has hemolytic action.This is owing to spirulina bioactive peptide, spirulina protein enzyme and after zymoplasm combines, make the proteolysis effect of zymoplasm and suppressed with the opsonic keying action of endotheliocyte thrombus, the platelet aggregation and the release of thrombin induction can be suppressed, effects such as the stimulation fibroblast proliferation of zymoplasm and smooth muscle cell contraction are suppressed, and having suppressed Zinc metallopeptidase Zace1, thrombin action is suppressed to bring into play good anticoagulant and thrombolytic effect and effect in the contractile function of damaged blood vessels in the thrombosis thereby make.In addition, the comprehensive action of the linolenic acid that spirulina itself contains, chlorophyll, superoxide dismutase and trace element also makes the present invention have the better effect that the microcirculation in human body obstacle is prevented and treated essential hypertension that improves.Apply the present invention to clinically, carry out " hemorheology " test, the whole blood viscosity of all tested personnel before taking the present invention: low cut, in cut, height cuts and all is higher than normal value, clinical diagnosis is a high blood viscosity disease.Take 1 of capsule of the present invention every day through every day, every contains the present invention's 0.15 gram, took continuously 1-2 month, through " hemorheology " test check, low cut, in cut, height cuts and all reduces to some extent and return to normal value, thus, prompting the present invention has preventive and therapeutic effect to high blood viscosity, and efficient is 100%.Apply the present invention to clinical vascular hypertension, take 1 of capsule of the present invention every day through every day, every contains the present invention's 0.15 gram, from the 3rd day, personnel's blood pressure of accepting test all obviously descended, thus, prompting the present invention has hypotensive effect, can be used for the control of essential hypertension.
Embodiment
Embodiment 1
(1) carries out spawn culture earlier, (subtilis has merchandise sales to get 1 of subtilis, the used subtilis of the present invention is bought from Institute of Microorganism, Academia Sinica) be inoculated on the nutrition nutrient agar substratum, nutrition nutrient agar substratum mainly is made up of following raw material: peptone 10 grams, extractum carnis 3 grams, agar 5 grams, sodium-chlor 5 gram adds sterilized water to 1000 milliliter, and accent PH to 7, the substratum that above-mentioned raw materials is mixed with is divided into seven, the spawn culture method was cultivated 64 hours under 35 ℃ of conditions routinely, merged the bacterial classification of being cultivated then and obtained bacteria culture fluid;
(2) getting 3 milliliters of above-mentioned bacteria culture fluids, to add 35 kilograms of spirulina powders and water ratio be to stir in 1: 1.3 the spirulina slurry, 37 ℃ condition bottom fermentations 18 hours, during stirred once in per 4 hours, allow the evenly fermentation of algae slurry;
(3) the above-mentioned spirulina that ferments is starched under the condition that places 3 ℃ and was stablized after-ripening 100 hours, and taking out in the temperature drying below 45 ℃ to water content is to get final product below 7%.
The present invention of method for preparing detects through Guangxi Center for Disease Control and Prevention, except that containing the composition that conventional spirulina contains, also contains spirulina bioactive peptide 32.4%, contains spirulina protein enzyme 1300U/g.
The present invention is carried out hemolytic test, test method by " in hospital's reagent test handbook " and the requirement of hemolytic test method visual method (Shandong science tech publishing house; 2001.08 editions) is carried out; detected result is: contain 0.5 the gram sample reaction soln 37 ℃ the effect 4 hours after, the most erythrocyte fragmentation in the solution.
The present invention of method for preparing is prepared into capsule, contains the present invention's 0.15 gram in every capsules.And capsule of the present invention is used for clinical work " hemorheology " test, subject person low cut, in cut, height cuts all above range of normal value, clinical diagnosis is a high blood viscosity disease, clinical symptom is that fatigue, giddy, insomnia, the heart are vexed.Capsule of the present invention of tested day for human beings clothes, serve on 2 months, again through " hemorheology " test check, lowly cut, in cut, height cuts and all reverts to normal value, clinical symptom disappearance, learn to handle by statistics, wherein, insomnia, tired, dizzy three, P ∠ 0.01, the vexed P ∠ 0.05 of the heart, the expression clinical symptom is clearly better, and the curative effect degree is better also more credible.
Embodiment 2
(1) (1) step with embodiment 1 is identical;
(2) getting 5 milliliters of above-mentioned bacteria culture fluids, to add 35 kilograms of spirulina powders and water ratio be to stir in 1: 1.3 the spirulina slurry, 37 ℃ condition lower seals fermentations 18 hours, during stirred once in per 4 hours, allow the evenly fermentation of algae slurry;
All the other steps are identical with embodiment 1.
The present invention of method for preparing detects through Guangxi Center for Disease Control and Prevention, except that containing the composition that conventional spirulina contains, also contains spirulina bioactive peptide 38.5%, contains spirulina protein enzyme 11000U/g.
Claims (3)
1, the spirulina that contains spirulina bioactive peptide, spirulina protein enzyme, mainly contain following composition: protein, amino acid, carbohydrate, fat, protein, robust fibre, vitamins C, vitamins B, vitamin-E, class Radix Dauci Sativae, inositol, chlorophyll, linolenic acid, superoxide dismutase, germanium, magnesium, calcium, iron, phosphorus, potassium, zinc, selenium, polysaccharide and moisture is characterized in that described spirulina water content is 7% also to contain the spirulina bioactive peptide of 15-40% and the spirulina protein enzyme of 1000-40000U/g when following.
2, the spirulina preparation method who contains spirulina bioactive peptide, spirulina protein enzyme as claimed in claim 1 is characterized in that described method is:
(1) subtilis is inoculated on the substratum, seven substratum of every bacterial classification inoculation, substratum is a nutrition nutrient agar substratum, constant volume is 1000 milliliters, cultivates 50-65 hour under 26-35 ℃ of condition;
(2) getting the weight ratio that above-mentioned cultured bacteria culture fluid 3-5 milliliter adds spirulina powder and water is 1: the 30-50 kilogram spirulina of 1.2-1.4 stirs in starching, 36-40 ℃ condition lower seal fermentation 12-18 hour, during regularly stir and allow the evenly fermentation of algae slurry;
(3) spirulina that ferments is starched under the condition that places 2-8 ℃ and was stablized after-ripening 60-120 hour, also can under this condition, preserve, and be to get final product below 7% again in the temperature drying below 45 ℃ to water content;
(4) above-mentioned dry spirulina is then obtained spirulina capsule with encapsulated; Above-mentioned dry spirulina is added that the appropriate amount of auxiliary materials compacting then obtains spirulina tablet in flakes.
3, the application that contains the spirulina of spirulina bioactive peptide, spirulina protein enzyme as claimed in claim 1 is characterized in that described spirulina has thrombolytic effect, and high blood viscosity is had preventive and therapeutic effect, is applied to the control of human body essential hypertension.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610159691 CN1923996A (en) | 2006-09-30 | 2006-09-30 | Apirulina containing spirulina bioactive peptide and spirulina proteinase, preparation method and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 200610159691 CN1923996A (en) | 2006-09-30 | 2006-09-30 | Apirulina containing spirulina bioactive peptide and spirulina proteinase, preparation method and application thereof |
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| CN1923996A true CN1923996A (en) | 2007-03-07 |
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| CN 200610159691 Withdrawn CN1923996A (en) | 2006-09-30 | 2006-09-30 | Apirulina containing spirulina bioactive peptide and spirulina proteinase, preparation method and application thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106901372A (en) * | 2016-12-28 | 2017-06-30 | 陕西天宝大豆食品技术研究所 | Full spirulina peptide nutrient food and preparation method thereof |
-
2006
- 2006-09-30 CN CN 200610159691 patent/CN1923996A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106901372A (en) * | 2016-12-28 | 2017-06-30 | 陕西天宝大豆食品技术研究所 | Full spirulina peptide nutrient food and preparation method thereof |
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