CN107586799B - A kind of malicious fermentation process of AFG1 production of aspergillus parasiticus - Google Patents
A kind of malicious fermentation process of AFG1 production of aspergillus parasiticus Download PDFInfo
- Publication number
- CN107586799B CN107586799B CN201711014844.1A CN201711014844A CN107586799B CN 107586799 B CN107586799 B CN 107586799B CN 201711014844 A CN201711014844 A CN 201711014844A CN 107586799 B CN107586799 B CN 107586799B
- Authority
- CN
- China
- Prior art keywords
- afg1
- aspergillus parasiticus
- medium
- mineral salt
- solid fermentation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The invention belongs to fermentation engineering fields, and in particular to a kind of malicious fermentation process of AFG1 production of aspergillus parasiticus.A kind of malicious fermentation process of AFG1 production of aspergillus parasiticus, this method comprises the following steps: a, the resurrection that aspergillus parasiticus spore is carried out using enriched medium are proliferated, b, solid fermentation culture is carried out using solid fermentation culture medium, contains the yeast extract for accounting for total weight of medium 1-3% in the component of enriched medium and solid fermentation culture medium.The toxin producing medium of AFG1 of the present invention solves the problems such as AFG1 concentration is low, uneven, time-consuming in the cereal of current natural infection mould.
Description
Technical field
The invention belongs to fermentation engineering fields, and in particular to a kind of malicious fermentation process of AFG1 production of aspergillus parasiticus.
Background technique
Aflatoxin (Aflatoxins, AFs) is produced by some of which such as aspergillus parasiticus, aspergillus flavus sum aggregate peak aspergillus
The mycetogenetic secondary metabolite of poison, has the three-induced effects such as carcinogenic, teratogenesis and mutagenesis.According to the literature, it has identified
AFs up to more than 20, wherein it is most commonly seen be AFs be aflatoxin B1 (AFB1), it is aflatoxin B 2 (AFB2), yellow
Aspertoxin G1 (AFG1), aflatoxin G 2 (AFG2).AFs can pollute the cereal such as corn, wheat, soybean and cottonseed and oil plant
Agricultural product can also remain in the agricultural and sideline products such as some DDGS, wheat bran, Soybean Meal and Cottonseed Meal.When the agricultural product of AFs pollution
When food as people, the health of people can be endangered, slight causes sub-health status, can seriously cause hepatic disease, causes dead
It dies；And when the agricultural and sideline product feeding animals of AFs pollution, the decline of Chang Yinqi breeding performonce fo animals, premunition reduce, and can lead when serious
It is lethal to die.Therefore, the pollution problem of AFs causes world many countries government and food safety monitoring machine in food and feed
The attention of structure sets the AFs limit standard in food and feed.The scientific research of AFs also by the concern of domestic and foreign scholars,
Corresponding test is carried out in the animals such as pig, milk cow and birds.
However, AFG1 maximum concentration is only 344ppb (Feng Jianlei, 2004) in the fermentation medium of domestic report, therefore
When carrying out the research of AFG1 related science, it is both needed to the AFG1 sample from external import price expensive (452/mg), experimentation cost is high, or
It needs to carry out commodity reservation, make troubles to related science research.
It can be the AFG1 toxicological test and AFs of animal in conclusion developing the fermentation medium and application method of AFG1
Food safety research provide test material.
Summary of the invention
The present invention provides a kind of AFG1 of aspergillus parasiticus to produce malicious fermentation process, for solving current natural infection mould
The problems such as AFG1 concentration is low, uneven, time-consuming in cereal.
The technical solution adopted by the present invention to solve the technical problems is:
A kind of malicious fermentation process of AFG1 production of aspergillus parasiticus, this method comprises the following steps:
A, it is proliferated using the resurrection that enriched medium carries out aspergillus parasiticus spore, the enriched medium each component is by weight
Measuring percentages includes: 10-25% potato, 4-10% glucose, 1-3% agar, 1-3% yeast extract, 0.5% grandidierite
Object salt, 0.2-0.5g/L chloramphenicol, surplus are water；
B, solid fermentation culture is carried out using solid fermentation culture medium, the solid fermentation culture medium each component is by weight
Percentages include: 41-70% hominy grits, 24-45% peanut meal, 4-10% sucrose, 0.4-1% composite mineral salt, 1-3% ferment
Female medicinal extract, after having prepared in proportion, adjusting solid fermentation moisture content in medium is 10-20%；
The component of composite mineral salt described in the enriched medium and solid fermentation culture medium is by percentage to the quality
Are as follows: 40-60% sodium nitrate, 5-15% magnesium sulfate, 5-15% potassium chloride, 15-30% dipotassium hydrogen phosphate, 3-8% zinc sulfate, 3-
Above-mentioned each mineral salt raw material is uniformly mixed, after crushed 80 mesh sub-sieves by 5% manganese sulfate, five aqueous ferrous sulfate of 0.2-0.8%
It mixes again, obtains composite mineral salt.
Preferably, the aspergillus parasiticus is posting for China General Microbiological culture presevation administrative center (CGMCC) offer
Raw Aspergillus (Aspergillus parasiticus) CGMCC NO.:3.0124.
Preferably, the enriched medium and solid fermentation culture medium, adjusting pH value with hydrochloric acid or sodium hydroxide is
It after 5.5-7.0, is placed in 2L conical flask, covers tampon, sterilizing 20min is carried out under conditions of 121-123 DEG C, 0.12MPa.
Preferably, the chloramphenicol ingredient in the enriched medium is after sterilizing, it is cooling to enriched medium
It is added and mixes after to 40 DEG C or less.
Inventor proves through a large amount of test display, parasitic under same culture conditions using enriched medium of the present invention
Time needed for aspergillus spore recovery only needs 2 days, and the recovery time than conventional medium shortens 4 days, and spore quantity improves 3
Times.Currently, when carrying out aspergillus parasiticus Zengjing Granule, conventional medium component are as follows: potato, glucose, agar and water, it should
Culture medium increasing bacterium speed is slow, and unit culture medium area spore quantity is few, and enriched medium efficiency provided by the invention obviously mentions
It is high.
Preferably, hominy grits described in the solid fermentation culture medium and peanut meal are through crushing, granularity reaches 20-
40 targets are quasi-.Solid fermentation culture medium institute's carbohydrate containing (hominy grits), protein (peanut meal), minerals (grandidierite
Object salt) and the nutriments such as somatomedin (yeast extract) meet the condition that aspergillus parasiticus efficiently produces poison just, and ratio is suitable
When, it can be achieved that aspergillus parasiticus efficiently produces AFG1.When carrying out solid fermentation culture, culture medium used in the prior art is only to adjust
The crushing maize of moisture, required fermented incubation time is apparently higher than solid fermentation culture medium of the invention, and the prior art is adopted
AFG1 concentration is substantially less than solid fermentation culture medium A FG1 concentration of the invention in culture medium.
Preferably, the optimum ratio of composite mineral salt are as follows: 40-53.5% sodium nitrate, 11.5-15% magnesium sulfate, 11-
14.5% potassium chloride, 17-22% dipotassium hydrogen phosphate, 3-6% zinc sulfate, 3-4% manganese sulfate, 0.5% 5 aqueous ferrous sulfate.
The sterilizing requirement of culture medium: the enriched medium and solid fermentation culture medium prepares (its in proportion respectively
In, the chloramphenicol ingredient in enriched medium is cooled to 40 DEG C or so additions to enriched medium and mixes after sterilizing),
After being 5.5-7.0 with hydrochloric acid or sodium hydroxide adjusting pH value, it is placed in 2L conical flask, covers tampon, at 121-123 DEG C,
0.12MPa, sterilize 20min, and each culture medium, which prepares work, to be terminated.
Enriched medium flat panel production: in Biohazard Safety Equipment, the enriched medium to have sterilized is upside down in by sterilizing
Diameter 10cm culture dish in, natural cooling.Then, enriched medium is placed in 37 DEG C of insulating boxs and is cultivated for 24 hours, asepsis growth
Person can use.
The method of the present invention uses above-mentioned solid fermentation culture medium, and the strain for selection of fermenting is aspergillus parasiticus bacterium (from China
General Microbiological Culture preservation administrative center, CGMCC NO.3.0124), zymotechnique is using conventional solid fermentation process.It presses
According to culture medium provided by the invention and cultural method, AFG1 concentration in the solid air dry matter of toxin producing medium is reachable
28956ppb, fermentation titer compared with prior art have significant progress.And this method fermentation efficiency is high, producing cost
It is low, the blank of the production field of China AFG1 is filled up, and can greatly reduce the research cost in the toxicological study field of AFG1.
The beneficial effects of the present invention are: the enriched medium that the present invention develops, no bacteria pollution interference, mould bring back to life proliferation
Fastly, conidium vitality is high, improves work efficiency.The solid fermentation culture medium that the present invention develops, nutrition is balanced, and fungus growth is prosperous
It contains, cultivation cycle is short, it is only necessary to which 10-12 days, opposite conventional corn culture medium, incubation time shortened 5-7 days, and AFG1 concentration is significant
It improves.Solid fermentation culture medium provided by the invention, mainly using hominy grits, peanut meal, sucrose, composite mineral salt and yeast leaching
Cream, raw material are easy to get, inexpensively.The opposite AFG1 from external import, the AFG1 price that culture medium provided by the invention is produced can drop
Low 90% or more.Fermentation process provided by the invention, simple and easy, strong operability.
Specific embodiment
Below by specific embodiment, technical scheme of the present invention will be further explained in detail.It should be appreciated that this hair
Bright implementation is not limited by the following examples, and the accommodation in any form made to the present invention and/or changed will all be fallen
Enter the scope of the present invention.
In the present invention, if not refering in particular to, all parts, percentage are unit of weight, and all equipment and raw material etc. are equal
It is commercially available or the industry is common.
Various embodiments of the present invention, it is bent with the parasitism that China General Microbiological culture presevation administrative center (CGMCC) is provided
Mould (Aspergillus parasiticus) CGMCC NO.:3.0124, is illustrated.
The preparation of embodiment 1-4 enriched medium
A kind of enriched medium for aspergillus parasiticus bacterium strain, after each component shown in table 1 is mixed, with hydrochloric acid and hydrogen
It is 5.5-7.0 that sodium hydroxide solution, which adjusts enriched medium pH value,.
Table 1
The preparation of embodiment 5-8 composite mineral salt
Sodium nitrate, magnesium sulfate, potassium chloride, dipotassium hydrogen phosphate, zinc sulfate, manganese sulfate and five water are taken according to the proportion in table 2
Above-mentioned mineral salt is uniformly mixed by ferrous sulfate, spare after mixing again after crushed 80 mesh sub-sieves, obtains Zengjing Granule
Composite mineral salt described in base and solid fermentation culture medium.
Table 2
Now by taking the Zengjing Granule based formulas of the composite mineral salt formula of embodiment 6 and embodiment 2 as an example, it is compound to prepare 100g
Mineral salt and 1L enriched medium.
Composite mineral salt (100g): 45.0g sodium nitrate, 11.5g magnesium sulfate, 13.0g chlorination are weighed with assay balance respectively
Potassium, 20.0g dipotassium hydrogen phosphate, 6.0g zinc sulfate, 4.0g manganese sulfate, five aqueous ferrous sulfate of 0.5g are uniformly mixed in beaker, then
Whole mixed mineral salt are put into supercentrifuge, 10min is crushed and mixes, put again after whole samples pass through 80 mesh sub-sieves
Set retain in brown bottle wide-mouth bottle it is spare.
Enriched medium (1L): potato cleans peeling, weighs 200g potato and is cut into small pieces, and 800mL boiling is added to boil 20-
30 minutes, until can be poked by glass bar, with 4 layers of filtered through gauze into beaker, filter residue was discarded, and into beaker, moisturizing is extremely
1000mL, then 60g glucose is added into beaker, 15g yeast extract, 5g composite mineral salt heats, adds 15g agar, after
Continuous heating stirring mixes, and after agar has dissolved, stirs evenly, and supplies moisture again to 1000 milliliters after slightly cooling down, uses 5mol/
The salt acid for adjusting pH value of L concentration is within the scope of 5.5-7.0.PH adjusting finishes, and is fitted into the conical flask that capacity is 2L, covers cotton
Plug, 121-123 DEG C at a temperature of, 0.12MPa, sterilize 20min.
In Biohazard Safety Equipment, after 0.5g/L chloramphenicol is added into 40 DEG C of enriched medium of sterilizing, then it is upside down in
In the diameter 10cm culture dish of sterilization treatment, culture medium, that is, solidifiable after cooling.Then, enriched medium is placed on 37 DEG C of incubators
For 24 hours, asepsis growth person can use for culture, and enriched medium preparation finishes.
The preparation of slant medium for aspergillus parasiticus culture presevation
According to aspergillus parasiticus enriched medium formula table, culture presevation culture medium needed for preparing.Prepared increasing bacterium is trained
Base is supported, 0.5g/L chloramphenicol is added under 60 DEG C of non-curdled appearances of culture medium, is upside down in the test tube of sterilizing, volume is about test tube
The 1/3 of capacity, tilts test tube, and the slant medium preparation of natural cooling, i.e. culture presevation finishes.
The preparation of embodiment 9-12 aspergillus parasiticus solid fermentation culture medium
According to the formula of table 3, solid fermentation culture medium needed for preparing.
Table 3
Raw material preparation: by hominy grits and peanut meal through crushing, it is desirable that the quasi- sub-sieve of 20 targets can be crossed, but 40 targets can not be crossed
Quasi- sub-sieve, i.e. granularity are between 20-40 mesh.
Now by taking the composite mineral salt formula of embodiment 6 and the component of embodiment 10 as an example, prepare 100g composite mineral salt and
1L enriched medium.
Composite mineral salt (100g): 45.0g sodium nitrate, 11.5g magnesium sulfate, 13.0g chlorination are weighed with assay balance respectively
Potassium, 20.0g dipotassium hydrogen phosphate, 6.0g zinc sulfate, 4.0g manganese sulfate, five aqueous ferrous sulfate of 0.5g are uniformly mixed in beaker, then
Whole mixed mineral salt are put into supercentrifuge, 10min is crushed and mixes, put again after whole samples pass through 80 mesh sub-sieves
Set retain in brown bottle wide-mouth bottle it is spare.
Solid fermentation culture medium (1kg).Weigh 500g hominy grits, 389g peanut meal, 80g sucrose, 6g composite mineral salt,
25g yeast extract, it is another to measure 100ml distilled water, above-mentioned raw materials are uniformly mixed, is placed in 3000ml conical flask, covers tampon,
At 121-123 DEG C, 0.12MPa, sterilize 30min.In Biohazard Safety Equipment, sterilized solid medium is upside down in and has been sterilized
Conical flask in, tiling is 60g/500ml triangular flask with a thickness of 1cm or useful load, covers sterilized bottle stopper.So far, it posts
Raw aspergillus fermentation solid culture medium preparation finishes.
The AFG1 of 13 aspergillus parasiticus of embodiment produces malicious fermented and cultured
1, the Multiplying culture method of aspergillus parasiticus strain
Strain is brought back to life: the aspergillus parasiticus freeze-dried powder 0.5mL physiological saline solution that will be saved in ampoule bottle, then with primary
Property asepsis injector draw 4mL lysate, uniform point sample is on the prepared enriched medium of embodiment 2, then uses sterile glass
Bacterium solution smearing is evenly distributed by stick.The enriched medium for having connect aspergillus parasiticus is placed in 35 DEG C of constant incubators and cultivates 46h, i.e.,
A large amount of mycelia can be grown, test requirements document is reached, strain resurrection finishes.
Distinguish composite mineral salt in 1,3,4 enriched medium of alternate embodiment using 6 composite mineral salt formula of embodiment to match
Enriched medium made from side, it is respectively 43h, 54h and 58h that aspergillus parasiticus, which brings back to life required time,.
Under similarity condition, the composite mineral salt formula of embodiment 5,7,8, difference 1 enriched medium of alternate embodiment are used
Middle composite mineral salt formula, it is respectively 42h, 47h and 51h that aspergillus parasiticus, which brings back to life required time,.
Using the composite mineral salt formula of embodiment 5,7,8, composite mineral salt in 2 enriched medium of alternate embodiment is distinguished
Formula, it is respectively 45h, 44h and 55h that aspergillus parasiticus, which brings back to life required time,.
Using the composite mineral salt formula of embodiment 5,7,8, composite mineral salt in 3 enriched medium of alternate embodiment is distinguished
Formula, it is respectively 41h, 46h and 52h that aspergillus parasiticus, which brings back to life required time,.
Use the composite mineral salt formula of embodiment 5,7,8, difference 4 enriched medium kind composite mineral salt of alternate embodiment
Formula, it is respectively 40h, 43h and 48h that aspergillus parasiticus, which brings back to life required time,.
2, aspergillus parasiticus fungi preservation: in Biohazard Safety Equipment, using aseptic inoculation needle, after sterilization and cooling, picking is above-mentioned
The bacterium solution of dissolution crosses in Z-shaped on the slant medium of aspergillus parasiticus culture presevation, covers sterilized ventilative tampon,
It is subsequently placed in 35 DEG C of constant incubators and cultivates 40h.It is to be generated grow more mycelia after, be placed in 4 DEG C of antistaling cabinets and save, often
It two months, is passed on according to this method primary.
3, aspergillus parasiticus bacterium is in fermentation medium top fermentation
Aspergillus parasiticus bacterium Multiplying culture: aseptic inoculation ring is used in Biohazard Safety Equipment, on a little normal saline flushing
The aspergillus parasiticus bacterium brought back to life is stated, and collects mycelia and spore liquid, then intensively crosses on multiple enriched medium, is placed in 35
46h is cultivated in DEG C constant incubator.Cover with the enriched medium plate of mycelia, it is possible to provide ferment required spore and mycelium.
The collection of aspergillus parasiticus bacterium mycelia and spore: it in Biohazard Safety Equipment, is rinsed with 5mL sterile saline and increases bacterium training
Base plate is supported, then carefully scrapes mycelia with sterile glass rod, mycelia and spore are collected in 250ml conical flask, with sterile life
It manages salt water and dilutes aspergillus parasiticus bacterium solution, until spore density is 1.2 × 106cfu/mL。
2mL aspergillus parasiticus mycelia and spore liquid are drawn with disposable syringe, bacterium solution is slowly sprayed on to embodiment 6 and is prepared
It in good solid medium, while being uniformly mixed using sterile glass rod, covers the ventilative tampon of sterilizing, be placed in relative humidity
85-90% is cultivated in the constant incubator that 35 DEG C of temperature.
The yeastiness of aspergillus parasiticus bacterium and incubator operating condition in conical flask are observed and recorded daily.
At interval of 3 days, fermentation medium is spun upside down using sterile glass rod in Biohazard Safety Equipment, paves fermentation training
Base is supported, so that culture medium is come into full contact with air, while spraying 5mL physiological saline, with the loss of moisture during afterfermentation.It covers
Sterile tampon is placed in 35 DEG C of constant incubators and continues to cultivate.
By 10-12 days fermented and cultureds, culture medium thoroughly fermented in conical flask, and culture medium agglomeration, mycelia color is in light
Yellow, fermented and cultured terminate.By culture in 121-123 DEG C, 0.12MPa, sterilize 20min, is steamed using freeze drier low pressure
Dry dry, dries pulverizing, the culture preparation containing AFG1 finish.
With the content of AFG1 in high performance liquid chromatography (GB/T 5009.23-2006) detection fermentation medium.Using reality
The solid fermentation culture medium of 6 composite mineral salt of example and embodiment 10 is applied, fermented and cultured 12 days, the concentration of AFG1 was reachable in air-drying sample
To 28956ppb.
Using the solid fermentation culture medium of embodiment 9,11,12, fermentation process is carried out under above-mentioned the same terms, air-dries training
Supporting AFG1 concentration in base is respectively 25781ppb, 23541ppb and 21234ppb.
It is compound in the solid fermentation culture medium of alternate embodiment 9 respectively using the composite mineral salt formula of embodiment 5,7,8
Mineral salt formula, fermentation process under the same conditions, in air-drying sample the concentration of AFG1 be respectively 23671ppb, 25553ppb and
22738ppb。
It is multiple in the solid fermentation culture medium of alternate embodiment 10 respectively using the composite mineral salt formula of embodiment 5,7,8
Close mineral salt formula, fermentation process under the same conditions, in air-drying sample the concentration of AFG1 be respectively 25381ppb, 21089ppb and
25337ppb。
It is multiple in the solid fermentation culture medium of alternate embodiment 11 respectively using the composite mineral salt formula of embodiment 5,7,8
Close mineral salt formula, fermentation process under the same conditions, in air-drying sample the concentration of AFG1 be respectively 21609ppb, 25855ppb and
21708ppb。
It is multiple in the solid fermentation culture medium of alternate embodiment 12 respectively using the composite mineral salt formula of embodiment 5,7,8
Close mineral salt formula, fermentation process under the same conditions, in air-drying sample the concentration of AFG1 be respectively 19691ppb, 25358ppb and
28031ppb。
Conclusion: the production poison concentration of the prior art is that 344 ± 11.5ppb AFG1 of the present invention is air-dried in the solid of toxin producing medium
Up to 28956ppb, the production poison concentration fermentation titer of 344 ± 11.5ppb AFG1 compared with prior art has aobvious concentration in object
The progress of work.This method fermentation efficiency is high, and producing cost is low, has filled up the blank of the production field of China AFG1, and can be very big
Reduce the research cost in the toxicological study field of AFG1.
Above-mentioned embodiment is only a preferred solution of the present invention, not the present invention is made in any form
Limitation, there are also other variations and modifications on the premise of not exceeding the technical scheme recorded in the claims.
Claims (5)
1. a kind of AFG1 of aspergillus parasiticus produces malicious fermentation process, it is characterised in that: this method comprises the following steps:
A, it is proliferated using the resurrection that enriched medium carries out aspergillus parasiticus spore, the enriched medium each component by weight hundred
Dividing than meter includes: 10-25% potato, 4-10% glucose, 1-3% agar, 1-3% yeast extract, 0.5% composite mineral salt, 0.2-
0.5g/L chloramphenicol, surplus are water；
B, solid fermentation culture, the solid fermentation culture medium each component percentage by weight are carried out using solid fermentation culture medium
It include: 41-70% hominy grits than meter, 24-45% peanut meal, 4-10% sucrose, 0.4-1% composite mineral salt, 1-3% yeast extract presses
After ratio has been prepared, adjusting solid fermentation moisture content in medium is 10-20%；
The component of composite mineral salt described in the enriched medium and solid fermentation culture medium is by percentage to the quality are as follows:
40-60% sodium nitrate, 5-15% magnesium sulfate, 5-15% potassium chloride, 15-30% dipotassium hydrogen phosphate, 3-8% zinc sulfate, 3-5% manganese sulfate,
Above-mentioned each mineral salt raw material is uniformly mixed, mixes, obtain again after crushed 80 mesh sub-sieves by five aqueous ferrous sulfate of 0.2-0.8%
To composite mineral salt；
The aspergillus parasiticus is the aspergillus parasiticus bacterium that China General Microbiological culture presevation administrative center (CGMCC) is provided
(Aspergillus parasiticus) CGMCC NO.:3.0124.
2. the AFG1 of aspergillus parasiticus according to claim 1 produces malicious fermentation process, it is characterised in that: the Zengjing Granule
Base and solid fermentation culture medium are placed in 2L conical flask after being 5.5-7.0 with hydrochloric acid or sodium hydroxide adjusting pH value, cover cotton
Plug, carries out sterilizing 20min under conditions of 121-123 DEG C, 0.12MPa.
3. the AFG1 of aspergillus parasiticus according to claim 2 produces malicious fermentation process, it is characterised in that:
Chloramphenicol ingredient in the enriched medium adds after enriched medium is cooled to 40 DEG C or less after sterilizing
Enter to mix.
4. the AFG1 of aspergillus parasiticus according to claim 1 produces malicious fermentation process, it is characterised in that: the solid fermentation
Through crushing, it is quasi- that granularity reaches 20-40 target for hominy grits described in culture medium and peanut meal.
5. the AFG1 of aspergillus parasiticus according to claim 1 produces malicious fermentation process, it is characterised in that: composite mineral salt it is excellent
Match ratio are as follows: 40-53.5% sodium nitrate, 11.5-15% magnesium sulfate, 11-14.5% potassium chloride, 17-22% dipotassium hydrogen phosphate, 3-6% sulphur
Sour zinc, 3-4% manganese sulfate, 0.5% 5 aqueous ferrous sulfate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711014844.1A CN107586799B (en) | 2014-12-04 | 2014-12-04 | A kind of malicious fermentation process of AFG1 production of aspergillus parasiticus |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410727872.8A CN104498557B (en) | 2014-12-04 | 2014-12-04 | The toxin producing medium of AFG1 a kind of and the AFG1 of aspergillus parasiticus produce malicious fermentation process |
| CN201711014844.1A CN107586799B (en) | 2014-12-04 | 2014-12-04 | A kind of malicious fermentation process of AFG1 production of aspergillus parasiticus |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410727872.8A Division CN104498557B (en) | 2014-12-04 | 2014-12-04 | The toxin producing medium of AFG1 a kind of and the AFG1 of aspergillus parasiticus produce malicious fermentation process |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107586799A CN107586799A (en) | 2018-01-16 |
| CN107586799B true CN107586799B (en) | 2019-04-26 |
Family
ID=52940007
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711014844.1A Active CN107586799B (en) | 2014-12-04 | 2014-12-04 | A kind of malicious fermentation process of AFG1 production of aspergillus parasiticus |
| CN201410727872.8A Expired - Fee Related CN104498557B (en) | 2014-12-04 | 2014-12-04 | The toxin producing medium of AFG1 a kind of and the AFG1 of aspergillus parasiticus produce malicious fermentation process |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410727872.8A Expired - Fee Related CN104498557B (en) | 2014-12-04 | 2014-12-04 | The toxin producing medium of AFG1 a kind of and the AFG1 of aspergillus parasiticus produce malicious fermentation process |
Country Status (1)
| Country | Link |
|---|---|
| CN (2) | CN107586799B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112877219A (en) * | 2021-01-29 | 2021-06-01 | 江西科技师范大学 | High-concentration cholesterol culture medium and preparation method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101603011A (en) * | 2009-06-18 | 2009-12-16 | 北京师范大学 | The method and the special strain therefore thereof that prepare the two glucosides of epipodophyllotoxin |
| CN102851334A (en) * | 2012-07-03 | 2013-01-02 | 浙江大学 | Fermentation medium and fermentation method of aflatoxin B1 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2387410B1 (en) * | 2009-01-14 | 2016-08-17 | Alltech, Inc. | Clay interlaced yeast compositions and methods of utilizing the same |
-
2014
- 2014-12-04 CN CN201711014844.1A patent/CN107586799B/en active Active
- 2014-12-04 CN CN201410727872.8A patent/CN104498557B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101603011A (en) * | 2009-06-18 | 2009-12-16 | 北京师范大学 | The method and the special strain therefore thereof that prepare the two glucosides of epipodophyllotoxin |
| CN102851334A (en) * | 2012-07-03 | 2013-01-02 | 浙江大学 | Fermentation medium and fermentation method of aflatoxin B1 |
Non-Patent Citations (1)
| Title |
|---|
| Toxigenic Potential of Aspergillus Species Occurring on Maize Kernels from Two Agro-Ecological Zones in Kenya;Sheila Okoth et al;《Toxins》;20121025;全文 |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104498557B (en) | 2018-01-05 |
| CN104498557A (en) | 2015-04-08 |
| CN107586799A (en) | 2018-01-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Li et al. | Spirulina industry in China: Present status and future prospects | |
| CN102060578B (en) | Method for producing microecological foliar fertilizer by utilizing probiotics and microalgae | |
| CN103396956B (en) | A kind of saccharomyces cerevisiae and its screening and culturing method and the method for bean pulp fermentation | |
| CN110342991A (en) | From the organic fertilizer of fermentation cordyceps sinensis | |
| CN101480226A (en) | Fungal fermentate feed addictive as well as preparation method and use thereof | |
| CN102178035A (en) | Method for fermenting and decomposing gossypol in cottonseed meal by composite strains | |
| CN101508961A (en) | Method of preparing yeast rich in copper | |
| CN108901615A (en) | Utilize the method for trollflower pharmacological property culture medium culture cordyceps mycelium | |
| CN107114758A (en) | A kind of ferment cordyceps sinensis D-mannitol powder and preparation method thereof | |
| CN106551250A (en) | A kind of preparation method of millet red koji | |
| CN103299824B (en) | Method for preparing phellinus igniarius sclertium from fresh, tender and sweet corn | |
| CN109288014A (en) | A kind of method of gingko coronoid process dissipate capsule bacterium liquid state fermentation and its product and the application of preparation | |
| CN105925493B (en) | One plant of bamboo parasitic fungus and its application in fermenting and producing hypocrellin | |
| CN101999673B (en) | Process for extracting dietary fibers from peony leaves by fermentation method | |
| CN108371239A (en) | A kind of feed addictive and its application for promoting animal growth | |
| CN107586799B (en) | A kind of malicious fermentation process of AFG1 production of aspergillus parasiticus | |
| CN104762232B (en) | A kind of microbial bacterial agent for chicken manure fermenting | |
| CN102851334B (en) | Fermentation medium and fermentation method of aflatoxin B1 | |
| CN107557402B (en) | A kind of malicious fermentation process of AFG2 production of aspergillus parasiticus | |
| CN104429583A (en) | Edible fungus liquid strain culturing method | |
| CN101653189A (en) | Natural plant small molecular group substance concentrate and production method thereof | |
| CN105586267B (en) | Produce the ganoderma lucidum mutagenic strain of ganoderma lucidum mycelium | |
| CN109422595A (en) | A kind of composite microbe fertilizer and its preparation method and application | |
| CN104694400B (en) | A kind of method that solid state fermentation prepares High color values monascus product | |
| CN103087887A (en) | Purple sweet potato health care wine and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |