CN1920451A - Refrigerator for liquified nitrogen biology - Google Patents
Refrigerator for liquified nitrogen biology Download PDFInfo
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- CN1920451A CN1920451A CN 200610051072 CN200610051072A CN1920451A CN 1920451 A CN1920451 A CN 1920451A CN 200610051072 CN200610051072 CN 200610051072 CN 200610051072 A CN200610051072 A CN 200610051072A CN 1920451 A CN1920451 A CN 1920451A
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- refrigerator
- temperature
- liquid nitrogen
- storeroom
- cover plate
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Abstract
The invention relates to a refrigerator of liquid nitrogen biology, which is formed by thermal-insulated frame (1), stainless steel liquid nitrogen container (7), liquid level instructor alarm (12), thermal-insulated cover plate (6), storage bank (13), stainless steel ventilate tube (5), fan (4), storage room, temperature sensor, and temperature controller (26); the refrigerator at -70--196Deg. C can store cell, organism block, and nucleic acid, with simple structure, lower cost, low power consumption and simple maintenance.
Description
Technical field
The present invention relates to a kind of liquid nitrogen refrigerator, relate in particular to biology, the medical laboratory is used under the low temperature, in the laboratory, be used to store cell under the low temperature about-70 ℃ or more, organize piece, essential equipment such as nucleic acid.
Background technology
Low temperature refrigerator (below 70 ℃) or liquid nitrogen biological container are to be used to store cell in the laboratory, organize piece, essential equipment such as nucleic acid, at present refrigerator be lower than below-25 ℃ domestic refrigerator seldom,-70 ℃ of biologic tests are more rare with refrigerator for being lower than, generally have only the liquid nitrogen of employing to reach this temperature as refrigerant type, the refrigerator that is used for the heater of biological refrigerated centrifuge or industrial employing is comparatively complicated, price is also expensive, aspect patent, be that CN200420017227.9 title " small-sized low-temperature biological frigorimeter " is introduced to being used for the small-sized low-temperature biological frigorimeter of the supper-fast cooling preservation biological sample of vitrifying as application number, comprise minipump, have the sealing shroud and the lid thereof of aspirating hole, small-sized Dewar container for liquefied nitrogen, heat exchanger, diffuser, temperature sensor, vacuum meter, device for digit-displaying, automatic controller etc.Adopt the pumped down method to make liquid-nitrogen boiling heat transfer and cold excessively, air is pressed again.Automatic controller is controlled vavuum pump and is compressed switch again, and overall process shows that automatically operating temperature is adjustable between subzero 196 ℃ to subzero 209 ℃.The vitrifying cryogenic freezing that is used for biological sample can reduce the protectant concentration of frozen liq, alleviates refrigeration toxicity and damage, improves the quality of refrigeration biological sample.Because to low-temperature biological experiment demand, the laboratory is to the liquid nitrogen biological container of its use, the freezing experiment device constantly improves, for the CN03267979.3 name is called " the cryovial fixator that is used for the liquid nitrogen frozen sample " utility model a kind of cryovial fixator that is used for the liquid nitrogen frozen sample is disclosed as application number, comprise wide-mouth stainless steel drum and suspension strop, described drum bottom is provided with more than one through hole, the drum top is provided with the elasticity square that is formed by the elastic component interconnection, this square is fixed in the edge on drum top, and the drum upper portion side wall is provided with at least four rope fastening holes that are symmetrical distribution; Described suspension strop lies in the rope fastening hole.It is introduced that this utility model can guarantee in liquid nitrogen steam refrigerating process, between batch and batch interior sample room freezing conditions consistent, the rate of temperature fall unanimity of freezing sample.Similarly patent also has much, but can not satisfy the requirement of experiment and production process.
At present, because this class low temperature refrigerator manufacturing requirements height, cost height, major part is an external product, power consumption is big in the use, noise is big, it is few to make some small-sized experiments and quantity, this class low temperature refrigerator purchasing price height, operating cost is also high, the inventor in practice process in order to overcome the shortcoming of using this class low temperature refrigerator, studied a kind of refrigerator for liquified nitrogen biology, can satisfy above biologic test requirement, price is also relatively cheap, by inventor's effort, finished this invention.
Summary of the invention
Refrigerator for liquified nitrogen biology of the present invention, it is mainly to be made up of the insulation shell 1 similar to general refrigerator, molten stainless steel nitrogen cascade 7, liquid nitrogen container fluid level indicator alarm 12, heat insulation cover plate 6, storage tank 13, stainless steel breather pipe 5, fan 4, storeroom, temperature sensor 9,10, temperature controller panel 26, and the refrigerator for liquified nitrogen biology of this structure is used as the essential equipment that stores cell, organizes piece, nucleic acid etc. in the laboratory under-70 ℃～-196 ℃ temperature.
Above-mentioned refrigerator for liquified nitrogen biology, its shell 1 and door 10 have insulation, heat-blocking action, and inside mainly is divided into upper and lower two parts, that is:
1. lower part has a molten stainless steel nitrogen cascade 7, be used to store liquid nitrogen 8, there is a liquid nitrogen to add hole 15 before the container, the outside clogs with Dewar bottle stopper 14 at ordinary times, the effect liquid nitrogen is added to and work as gas nitrogen usefulness can from then on overflow when too much the time, device has the fluid level indicator alarm 12 of liquid nitrogen in the liquid nitrogen container 7, the liquid nitrogen container top covers with heat insulation cover plate 6, between liquid nitrogen 8 and the heat insulation cover plate 6 certain space is arranged, two ends, the heat insulation cover plate left and right sides respectively have 2～3 holes can allow stainless steel breather pipe 5 pass near the edge, have made the hole 19 of a plurality of rules on the heat insulation cover plate, and quantity equals storage tank 13 numbers, being used to insert storage tank 13 also can seal, storage tank 13 adopts stainless steel to make, and the little case of door 24 is arranged, and multilayer insulating panel 22 can be arranged, the end of each dividing plate, is porose, the height of storage tank is after storage tank is inserted the respective aperture of heat insulation cover plate 6, and each can be immersed in the liquid nitrogen 8 fully every sample reserve 23 in the case, puts into or when taking out sample 23, storage tank is upwards mentioned, open chamber door and get final product;
2. the top of refrigerator be heat insulation cover plate 6 with top, 2～3 layers of storeroom are topmost arranged, the distance of the following identity distance insulation cover plate 6 of the storeroom in below is its height of space, should be greater than the height of storage tank 13 so that mention or insert storage tank 13;
Stainless steel breather pipe 5 is a closed conduit, a left side arises from 6 times liquid nitrogen 8 of heat insulation cover plate, fan 4 is housed in the ascending tube 5, along the refrigerator left side wall vertically upward, inserts storeroom and be the snakelike corresponding storeroom inner top that walks in, pass storeroom to the right, along the refrigerator right side wall vertically downward, terminate under the right side respective aperture of heat insulation cover plate, go up 26 controls of temperature control panel panel through moving into one's husband's household upon marriage, set the working time of fan 4 and can adjust the storage indoor temperature, allow it reduce to setting value.
Refrigerator for liquified nitrogen biology of the present invention, can keep storing indoor temperature is that-30 ℃ of-90 ℃ of left and right sides are adjustable, and the temperature in the bottom storage tank 13 are-196 ℃, satisfy the requirement of biological sample storage temperature, integrate the advantage of low temperature refrigerator and liquid nitrogen biological container.
Refrigerator for liquified nitrogen biology of the present invention simple in structure, it is not high to make the technical conditions that require, and cost is low, during operation, power consumption is little, noise is low, safeguards that simply maintenance cost is low, easy to use, as long as add liquid nitrogen as required or regularly, just can constantly move, satisfy the needs of various biological sample storages.Because it is not high to make the technical conditions that require, cost is low, and operation expense is low, this product was both practical, and price can be decided properly, so, the appearance of this product can promote the more extensive engineering ground of biological study work to carry out, and the benefit of product energy-saving and environmental protection simultaneously is also very outstanding.
Description of drawings
Fig. 1 is the schematic diagram of refrigerator for liquified nitrogen biology front view.
Fig. 2 looks the schematic diagram of cutaway view for a refrigerator for liquified nitrogen biology left side.
Fig. 3 looks the schematic diagram of cutaway view for the refrigerator for liquified nitrogen biology right side.
Fig. 4 is the schematic diagram at refrigerator for liquified nitrogen biology storeroom top.
Fig. 5 is the schematic diagram of refrigerator for liquified nitrogen biology vertical view cutaway drawing.
Fig. 6 is the schematic diagram of refrigerator for liquified nitrogen biology storage tank zoomed-in view.
Among above Fig. 1 26 is temperature control panel, 14 Dewar bottle stoppers.
Among above Fig. 21 is the shell of the insulation of refrigerator for liquified nitrogen biology, the 2nd, and storeroom A, the 3rd, storeroom B, the 4th, fan, the 5th, breather pipe, the 6th, heat insulation cover plate, the 7th, the molten stainless steel nitrogen cascade, the 8th, liquid nitrogen, the 9th, thermoreceptor A, the 10th, refrigerator doors, the 11st, thermoreceptor B, the 12nd, liquid nitrogen container liquid level indication alarm, the 13rd, storage tank, the 25th, the insulation layer in the outcase of refrigerator.
Among above Fig. 31 is the shell of the insulation of refrigerator for liquified nitrogen biology, the 2nd, and storeroom A, the 3rd, storeroom B, the 5th, breather pipe, the 6th, heat insulation cover plate, the 7th, molten stainless steel nitrogen cascade, the 8th, liquid nitrogen, the 9th, thermoreceptor A, the 10th, refrigerator doors, the 13rd, storage tank, the 14th, Dewar bottle stopper, the 15th, liquid nitrogen adds the hole, and the 25th, the insulation layer in the outcase of refrigerator.
Among above Fig. 4 16 is the snakelike heat exchange tubes at storeroom top, and each storeroom top has.
Among above Fig. 5 17 is breather pipe outlet A, the 18th, and breather pipe outlet B, the 19th, storage tank patchhole, the 20th, breather pipe inlet A, the 21st, breather pipe inlet B.
Among above Fig. 6 22 is the porose dividing plates of storage tank, the 23rd, and reserve, the 24th, storing chamber door.
Being installed in for temperature control panel 26 in the schematic diagram of refrigerator for liquified nitrogen biology front view at Fig. 1 can be by setting the conditions such as running temperature of control refrigerator on the door of refrigerator for liquified nitrogen biology on panel, this is a comparatively mature technique, and the present invention does not list its line map and structure.
Look shell 1 and door 10 inside of the schematic diagram of cutaway view at the insulation of refrigerator for a refrigerator for liquified nitrogen biology left side at Fig. 2, be divided into two parts by heat insulation cover plate 6, two storeroom 2A and storeroom 3B are arranged at this figure top, snakelike heat exchange tube 16 is arranged respectively at each storeroom top, wherein stainless steel breather pipe 5 (ascender) passes 6, in every pipe of breather pipe 5 (ascender), adjustment fan 4 is installed, its switch and running time set temperature by the temperature control panel of installing on the door 26 and control, heat insulation cover plate 6 down is a liquid nitrogen container liquid level indication alarm 12 with the lower part again, molten stainless steel nitrogen cascade 7, liquid nitrogen 8, storage tank 13;
The refrigerator of two storeroom 2A, 3B of herein drawing is made example, if relatively large refrigerator for liquified nitrogen biology can be increased to storeroom 3, the up-downgoing passage, pipe is corresponding is increased to 3.
Look in the schematic diagram of cutaway view for the refrigerator for liquified nitrogen biology right side at Fig. 3, that sees after analysing and observe is similar with Fig. 2, two storeroom 2A and storeroom 3B are arranged at this figure top, snakelike heat exchange tube 16 is arranged respectively at each storeroom top, pass 6 from up gas by stainless steel breather pipe 5 (descender), or breather pipe 5 (descender) they be passed in 20 on the heat insulation cover plate 6,21 enter heat insulation cover plate below 6, this part is the space between the liquid nitrogen 8 of molten stainless steel nitrogen cascade 7, the adjustment of storeroom 2A and storeroom 3B is arrived the temperature of setting, liquid nitrogen container liquid level indication alarm 12, how much set liquid nitrogen liquid level alarm height according to storings in the storage tank 13, if liquid nitrogen 8 can not biological specimen that submergence is stored up, can not reach the preservation temperature, liquid nitrogen container liquid level indication alarm 12 display alarm signal on control panel 26, require administrative staff by opening Dewar bottle stopper 14, add hole 15 from liquid nitrogen and add liquid nitrogen, liquid level up to liquid nitrogen 8 reaches home, the heat preserving and insulating material of insulation layer 25 its filling in the outcase of refrigerator guarantees that inner heat exchange with environment is reduced to very low level, satisfies the needs of normal operation.
At Fig. 4 is in the schematic diagram at refrigerator for liquified nitrogen biology storeroom top, the nitrogen of expression low temperature enters the serpentine heat exchanger 16 of storeroom 2 or 3 from the stainless steel up mouth of pipe 5 arrows of ventilating, descending from stainless steel ventilation down pipe 5 arrow exits, through heat exchange, with the adjustment of storeroom 2A, 3B to design temperature.
At Fig. 5 is in the schematic diagram of refrigerator for liquified nitrogen biology vertical view cutaway drawing, near the signal of position cutaway view heat insulation cover plate 6, express stainless steel ventilation 5, should install and insert the through hole of being opened on the heat insulation cover plate 6 17,18,20,21 and sealing prevent that gas from leaking, on heat insulation cover plate 6, also opened the hole 19 that to place storage tank 13 a plurality of holes have been arranged, in addition, can see on the dividing plate 24 of storage tank 13 through hole is arranged, be in order to guarantee storage tank 13 internal temperature unanimities, 6 of storage tank 13 and heat insulation cover plates are used washer sealing, storage tank 13, take out or place behind the biological specimen container that stores up 23, all require to reinstall storage tank hole 19 the airtight separation that makes heat insulation cover plate 6 keep two parts up and down of refrigerator inside.
Fig. 6 is the schematic diagram of refrigerator for liquified nitrogen biology storage tank 13 zoomed-in views, on the storage tank top cover except the heat preserving and insulating material the same with heat insulation cover plate 6 is installed, storage tank top cover handle (omit in the accompanying drawings and do not draw) also is installed, the wicket of opening 24 is placed on the container 23 of biological sample reserve on the dividing plate 22 of through hole, close wicket, put down the hole 19 of the placement storage tank that storage tank 13 is opened from heat insulation cover plate 6, be immersed in the liquid nitrogen 8, the temperature of biological sample container 23 is rapidly ℃ consistent with the temperature-196 of liquid nitrogen.
The specific embodiment
Embodiment 1: will have the frozen pipe 23 that needs frozen fibroblast suspension and place general refrigerator to preserve 30 minutes for 4 ℃, go to then and place 24 hours (design temperature is for-70 ℃) among the refrigerator for liquified nitrogen biology storeroom 2A, put into storage tank 13 again, immerse in the liquid nitrogen 8, rapidly ℃ consistent with the temperature-196 of liquid nitrogen, promptly finished fibroblastic frozen.
Embodiment 2: the frozen pipe 23 for the treatment of frozen keratinocyte suspension will be housed place general refrigerator, preserved 30 minutes down at 4 ℃, then, frozen pipe 23 is gone in the refrigerator for liquified nitrogen biology storeroom, design temperature on refrigerator control panel 26-30 ℃, frozen pipe frozen 2 hours at refrigerator storeroom, then, design temperature is changed into-60 ℃, frozen 4 hours, subsequently design temperature is changed into-90 ℃, frozen 18 hours, take out, put into storage tank 13 again, close storing chamber door 24, storage tank is inserted in the respective aperture 19 of heat insulation cover plate 6, immerse in the liquid nitrogen 8, frozen pipe 23 rapidly and temperature-196 ℃ the temperature of liquid nitrogen, refrigerator doors 10 is closed, promptly finished the frozen of keratinocyte.
Embodiment 3: when liquid nitrogen container liquid level indication alarm 12 is reported to the police, by liquid nitrogen being transported to refrigerator for liquified nitrogen biology with Dewar bottle from suppliers, the refrigerator for liquified nitrogen biology liquid nitrogen being added the Dewar bottle stopper 14 in hole 15 opens, lay a funnel, liquid nitrogen in the Dewar bottle is added in molten stainless steel nitrogen cascade 7 by funnel, be added to scheduled volume until liquid nitrogen 8.
Claims (1)
- Stainless steel breather pipe (5) is a closed conduit, a left side arises under the heat insulation cover plate (6) on the liquid nitrogen (8), fan (4) is housed in the ascending tube (5), along the refrigerator left side wall vertically upward, inserts storeroom and be the snakelike corresponding storeroom inner top that walks in, pass storeroom to the right, along the refrigerator right side wall vertically downward, terminate under the right side respective aperture of heat insulation cover plate, go up temperature control panel panel (26) control through moving into one's husband's household upon marriage, set the working time of fan (4), can adjust the storage indoor temperature and reduce to setting value.3, a kind of refrigerator for liquified nitrogen biology according to claim 1, it is characterized in that can keeping storing indoor temperature and be-30 ℃～-90 ℃ adjustable, and the interior temperature of bottom storage tank (13) is-196 ℃, satisfy the requirement of biological sample storage temperature, integrate the advantage of low temperature refrigerator and liquid nitrogen biological container.Stainless steel breather pipe (5) is a closed conduit, a left side arises under the heat insulation cover plate (6) on the liquid nitrogen (8), fan (4) is housed in the ascending tube (5), along the refrigerator left side wall vertically upward, inserts storeroom and be the snakelike corresponding storeroom inner top that walks in, pass storeroom to the right, along the refrigerator right side wall vertically downward, terminate under the right side respective aperture of heat insulation cover plate, go up temperature control panel panel (26) control through moving into one's husband's household upon marriage, set the working time of fan (4), can adjust the storage indoor temperature and reduce to setting value.3, a kind of refrigerator for liquified nitrogen biology according to claim 1, it is characterized in that can keeping storing indoor temperature and be-30 ℃～-90 ℃ adjustable, and the interior temperature of bottom storage tank (13) is-196 ℃, satisfy the requirement of biological sample storage temperature, integrate the advantage of low temperature refrigerator and liquid nitrogen biological container.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200610051072A CN100595499C (en) | 2006-05-25 | 2006-05-25 | Refrigerator for liquified nitrogen biology |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200610051072A CN100595499C (en) | 2006-05-25 | 2006-05-25 | Refrigerator for liquified nitrogen biology |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1920451A true CN1920451A (en) | 2007-02-28 |
| CN100595499C CN100595499C (en) | 2010-03-24 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200610051072A Expired - Fee Related CN100595499C (en) | 2006-05-25 | 2006-05-25 | Refrigerator for liquified nitrogen biology |
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| Country | Link |
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| CN (1) | CN100595499C (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102046774B (en) * | 2007-11-09 | 2013-06-19 | 普莱克斯技术有限公司 | Method and system for controlled rate freezing of biological material |
| CN104949431A (en) * | 2015-06-01 | 2015-09-30 | 中国农业大学 | Refrigeration device |
| CN106477175A (en) * | 2016-11-24 | 2017-03-08 | 徐伟强 | Gas phase liquified nitrogen biology holding vessel |
| CN106829202A (en) * | 2017-01-13 | 2017-06-13 | 蔡晨宇 | The method of constant low temperature storage system and removal Dewar fog |
| CN107228518A (en) * | 2017-06-04 | 2017-10-03 | 王玉杰 | Stem cell injecta transporting preservation device |
| CN111595079A (en) * | 2020-05-15 | 2020-08-28 | Tcl家用电器（合肥）有限公司 | Fixing device and refrigerator |
-
2006
- 2006-05-25 CN CN200610051072A patent/CN100595499C/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102046774B (en) * | 2007-11-09 | 2013-06-19 | 普莱克斯技术有限公司 | Method and system for controlled rate freezing of biological material |
| CN104949431A (en) * | 2015-06-01 | 2015-09-30 | 中国农业大学 | Refrigeration device |
| CN106477175A (en) * | 2016-11-24 | 2017-03-08 | 徐伟强 | Gas phase liquified nitrogen biology holding vessel |
| CN106829202A (en) * | 2017-01-13 | 2017-06-13 | 蔡晨宇 | The method of constant low temperature storage system and removal Dewar fog |
| CN106829202B (en) * | 2017-01-13 | 2019-05-31 | 北京华晨世纪生物技术有限公司 | Constant low temperature storage system and the method for removing Dewar fog |
| CN107228518A (en) * | 2017-06-04 | 2017-10-03 | 王玉杰 | Stem cell injecta transporting preservation device |
| CN111595079A (en) * | 2020-05-15 | 2020-08-28 | Tcl家用电器（合肥）有限公司 | Fixing device and refrigerator |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100595499C (en) | 2010-03-24 |
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Granted publication date: 20100324 Termination date: 20110525 |