CN1907138B - Sandy land grapefruit juice extract, extraction process and application thereof - Google Patents
Sandy land grapefruit juice extract, extraction process and application thereof Download PDFInfo
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- CN1907138B CN1907138B CN2006100370150A CN200610037015A CN1907138B CN 1907138 B CN1907138 B CN 1907138B CN 2006100370150 A CN2006100370150 A CN 2006100370150A CN 200610037015 A CN200610037015 A CN 200610037015A CN 1907138 B CN1907138 B CN 1907138B
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Abstract
The invention discloses a sand grapefruit juice extract and extracting method, which comprises the following parts: 5-methoxy furocoumarin, 5- methoxy-8-isoamylene oxy-furocoumarin, bergamot, 6', 7'-epoxy furocoumarin, 6', and 7'-dihydroxy furocoumarin, 2', and 3'-epoxy decursin dipolymer, 6', 7'-epoxy furocoumarin dipolymer, 6', 7'-dihydroxy furocoumarin dipolymer.
Description
Technical field
The present invention relates to a kind of sandy land grapefruit juice extract, the invention still further relates to the extracting method of this sandy land grapefruit juice extract simultaneously and using.
Background technology
Modern biology and study of pharmacy result show, edible grapefruit juice can reduce cholesterol in human body content, prevention high fat of blood and hypertensive generation, its main active factors is the furocoumarin analog derivative, particularly a kind ofly be called 6 ', 7 '-DHB (6 ', 7 '-dihydroxybergamottin, 6 ', 7 '-dihydroxy bergamot element) furocoumarin and analog thereof, can promote the absorption level of prohormone and other material, suppress the activity of Cytochrome P450 drug metabolic enzyme CYP3A4, can strengthen the oral bioavailability rate of some drugs clinically.Grapefruit juice also has key enzyme---the effect of regulating in the cholesterol metabolic of LCA (LCAT) relative activity simultaneously, plays the effect of reducing blood lipid.
Present 6 ', 7 '-DHB and analog thereof be rich in the citrous pulp of Rutaceae, and its extracting method has organic solvent method, supercritical carbon dioxide extraction method and adsorption chromatography.China's shatian pomelo aboundresources, therefrom extraction has norcholesterol and hypolipidemic activity composition, for the shatian pomelo resource of developing mountain areas, improves shatian pomelo resource added value, has economic benefit and social benefit widely.
Summary of the invention
The object of the present invention is to provide a kind of sandy land grapefruit juice extract.
Another object of the present invention is to provide the extracting method of sandy land grapefruit juice extract.
The present invention also aims to provide the application of shatian pomelo extract in preparation norcholesterol and blood fat reducing healthcare food.
The invention provides a kind of sandy land grapefruit juice extract, it is characterized in that containing mass percent content and be 0.8% relative molecular mass and be 216,300,338,354,372,574 furocoumarin derivative, its main component is 5-methoxyl group furocoumarin, 5-methoxyl group-8-iso-amylene oxygen base furocoumarin, bergamot element, 6`, 7`-epoxy bergamot element, 6`, 7`-dihydroxy bergamot element, 2`, 3`-epoxy peucedanin dimer; It prepares as follows: (1) fresh MeiZhou,GuangDong third village shatian pomelo peeling, get shatian pomelo shaddock meat, squeeze juice on juice extractor; (2) 5000 leave the heart separates grapefruit juice, removes solid residue, obtains the grapefruit juice crude extract; (3) ethyl acetate of getting 5 times of clear liquid volumes extracts the gained clear liquid, repeat 3 times, combining extraction liquid, decompression concentrates, the sandy land grapefruit juice extract concentrate.
The extracting method of above-mentioned sandy land grapefruit juice extract may further comprise the steps:
(1) fresh MeiZhou,GuangDong third village shatian pomelo peeling is got shatian pomelo shaddock meat, squeeze juice on juice extractor;
(2) 5000 leave the heart separates grapefruit juice, removes solid residue, obtains the grapefruit juice crude extract;
(3) ethyl acetate of getting 5 times of clear liquid volumes extracts the gained clear liquid, repeat 3 times, combining extraction liquid, decompression concentrates, the sandy land grapefruit juice extract concentrate.
A kind of sandy land grapefruit juice extract provided by the invention, it is characterized in that containing mass percent content and be 0.5% relative molecular mass and be 338,354,372,708 furocoumarin derivative, its main component is bergamot element, 6`, 7`-epoxy bergamot element, 6`, 7`-dihydroxy bergamot element, 6`, the plain dimer of 7`-epoxy bergamot; It prepares as follows: (1) fresh MeiZhou,GuangDong crosses the shatian pomelo peeling in vain, gets shatian pomelo shaddock meat, squeeze juice on juice extractor; (2) 5000 leave the heart separates grapefruit juice, removes solid residue, obtains the grapefruit juice crude extract; (3) n-butanol of getting 2 times of clear liquid volumes extracts the gained clear liquid, repeat 3 times, combining extraction liquid, decompression concentrates, the sandy land grapefruit juice extract concentrate.
The extracting method of above-mentioned sandy land grapefruit juice extract may further comprise the steps:
(1) fresh MeiZhou,GuangDong crosses the shatian pomelo peeling in vain, gets shatian pomelo shaddock meat, squeeze juice on juice extractor;
(2) 5000 leave the heart separates grapefruit juice, removes solid residue, obtains the grapefruit juice crude extract;
(3) n-butanol of getting 2 times of clear liquid volumes extracts the gained clear liquid, repeat 3 times, combining extraction liquid, decompression concentrates, the sandy land grapefruit juice extract concentrate.
A kind of sandy land grapefruit juice extract provided by the invention, it is characterized in that containing mass percent content and be 0.7% relative molecular mass and be 338,354,372,708,726 furocoumarin derivative, its main component is bergamot element, 6`, 7`-epoxy bergamot element, 6`, 7`-dihydroxy bergamot element, 6`, plain dimer of 7`-epoxy bergamot and 6`, the plain dimer of 7`-dihydroxy bergamot; It prepares as follows: (1) fresh MeiZhou,GuangDong crosses the shatian pomelo peeling in vain, gets shatian pomelo shaddock meat, squeeze juice on juice extractor; (2) 5000 leave the heart separates grapefruit juice, removes solid residue, obtains the grapefruit juice crude extract; (3) ether of getting 5 times of clear liquid volumes extracts the gained clear liquid, repeat 3 times, combining extraction liquid, the water-bath heating concentrates, the sandy land grapefruit juice extract concentrate.
The extracting method of above-mentioned sandy land grapefruit juice extract may further comprise the steps:
(1) fresh MeiZhou,GuangDong crosses the shatian pomelo peeling in vain, gets shatian pomelo shaddock meat, squeeze juice on juice extractor;
(2) 5000 leave the heart separates grapefruit juice, removes solid residue, obtains the grapefruit juice crude extract;
(3) ether of getting 5 times of clear liquid volumes extracts the gained clear liquid, repeat 3 times, combining extraction liquid, the water-bath heating concentrates, the sandy land grapefruit juice extract concentrate.
Sandy land grapefruit juice extract provided by the invention; can be mixed with health food or beverage with one or more pharmaceutically acceptable carriers (auxiliary material) with norcholesterol and effect for reducing blood fat; wherein said norcholesterol and effect for reducing blood fat; be meant and have the serum total cholesterol of reduction content, triglyceride content, the effects such as relative activity that improve LCA.
Sandy land grapefruit juice extract provided by the present invention and extracting method thereof have following characteristics: 1. raw material is easy to obtain, and is cheap; 2. agents useful for same is common agents, and can re-use after recovery to reduce production costs; 3. operating condition is simpler, is easy to realize suitability for industrialized production; 4. the residue after extracting also can change into high-quality feed, has significant commercial advantage; 5. can be developed into the health food with norcholesterol and blood fat reducing function, have low price, drug effect is remarkable, and characteristics such as instant are significant aspect supplemental treatment and prevention high cholesterol and high fat of blood.
The specific embodiment
To be easier to understand the present invention with reference to the following example, and provide embodiment and be in order to illustrate the present invention, rather than in order to limit the scope of the invention.
Embodiment 1
(1) fresh MeiZhou,GuangDong third village shatian pomelo peeling is got shatian pomelo shaddock meat 1kg, squeeze juice on juice extractor;
(2) 5000 leave the heart separates grapefruit juice, removes solid residue, obtains the grapefruit juice crude extract;
(3) ethyl acetate of getting 5 times of clear liquid volumes extracts the gained clear liquid, repeat 3 times, combining extraction liquid, decompression concentrates, sandy land grapefruit juice extract concentrate 5g.
Analyze above-mentioned sandy land grapefruit juice extract, wherein contain mass percent content and be 0.8% relative molecular mass and be 216,300,338,354,372,574 furocoumarin derivative, its main component is 5-methoxyl group furocoumarin, 5-methoxyl group-8-iso-amylene oxygen base furocoumarin, bergamot element, 6`, 7`-epoxy bergamot element, 6`, 7`-dihydroxy bergamot element, 2`, 3`-epoxy peucedanin dimer.
Embodiment 2
(1) fresh MeiZhou,GuangDong crosses the shatian pomelo peeling in vain, gets shatian pomelo shaddock meat 1kg, squeeze juice on juice extractor;
(2) 5000 leave the heart separates grapefruit juice, removes solid residue, obtains the grapefruit juice crude extract;
(3) n-butanol of getting 2 times of clear liquid volumes extracts the gained clear liquid, repeat 3 times, combining extraction liquid, decompression concentrates, sandy land grapefruit juice extract concentrate 4g.
Analyze above-mentioned sandy land grapefruit juice extract, wherein contain mass percent content and be 0.5% relative molecular mass and be 338,354,372,708 furocoumarin derivative, its main component is bergamot element, 6`, 7`-epoxy bergamot element, 6`, 7`-dihydroxy bergamot element, 6`, the plain dimer of 7`-epoxy bergamot.
Embodiment 3
(1) fresh MeiZhou,GuangDong crosses the shatian pomelo peeling in vain, gets shatian pomelo shaddock meat 1kg, squeeze juice on juice extractor;
(2) 5000 leave the heart separates grapefruit juice, removes solid residue, obtains the grapefruit juice crude extract;
(3) ether of getting 5 times of clear liquid volumes extracts the gained clear liquid, repeat 3 times, combining extraction liquid, the water-bath heating concentrates, sandy land grapefruit juice extract concentrate 4.6g.
Analyze above-mentioned sandy land grapefruit juice extract, wherein contain mass percent content and be 0.7% relative molecular mass and be 338,354,372,708,726 furocoumarin derivative, its main component is bergamot element, 6`, 7`-epoxy bergamot element, 6`, 7`-dihydroxy bergamot element, 6`, plain dimer of 7`-epoxy bergamot and 6`, the plain dimer of 7`-dihydroxy bergamot.
Claims (9)
1. sandy land grapefruit juice extract, it is characterized in that containing mass percent content and be 0.8% relative molecular mass and be 216,300,338,354,372,574 furocoumarin derivative, its main component is 5-methoxyl group furocoumarin, 5-methoxyl group-8-iso-amylene oxygen base furocoumarin, bergamot element, 6`, 7`-epoxy bergamot element, 6`, 7`-dihydroxy bergamot element, 2`, 3`-epoxy peucedanin dimer; It prepares as follows: (1) fresh MeiZhou,GuangDong third village shatian pomelo peeling, get shatian pomelo shaddock meat, squeeze juice on juice extractor; (2) 5000 leave the heart separates grapefruit juice, removes solid residue, obtains the grapefruit juice crude extract; (3) ethyl acetate of getting 5 times of clear liquid volumes extracts the gained clear liquid, repeat 3 times, combining extraction liquid, decompression concentrates, the sandy land grapefruit juice extract concentrate.
2. sandy land grapefruit juice extract, it is characterized in that containing mass percent content and be 0.5% relative molecular mass and be 338,354,372,708 furocoumarin derivative, its main component is bergamot element, 6`, 7`-epoxy bergamot element, 6`, 7`-dihydroxy bergamot element, 6`, the plain dimer of 7`-epoxy bergamot; It prepares as follows: (1) fresh MeiZhou,GuangDong crosses the shatian pomelo peeling in vain, gets shatian pomelo shaddock meat, squeeze juice on juice extractor; (2) 5000 leave the heart separates grapefruit juice, removes solid residue, obtains the grapefruit juice crude extract; (3) n-butanol of getting 2 times of clear liquid volumes extracts the gained clear liquid, repeat 3 times, combining extraction liquid, decompression concentrates, the sandy land grapefruit juice extract concentrate.
3. sandy land grapefruit juice extract, it is characterized in that containing mass percent content and be 0.7% relative molecular mass and be 338,354,372,708,726 furocoumarin derivative, its main component is bergamot element, 6`, 7`-epoxy bergamot element, 6`, 7`-dihydroxy bergamot element, 6`, plain dimer of 7`-epoxy bergamot and 6`, the plain dimer of 7`-dihydroxy bergamot; It prepares as follows: (1) fresh MeiZhou,GuangDong crosses the shatian pomelo peeling in vain, gets shatian pomelo shaddock meat, squeeze juice on juice extractor; (2) 5000 leave the heart separates grapefruit juice, removes solid residue, obtains the grapefruit juice crude extract; (3) ether of getting 5 times of clear liquid volumes extracts the gained clear liquid, repeat 3 times, combining extraction liquid, the water-bath heating concentrates, the sandy land grapefruit juice extract concentrate.
4. the extracting method of the described sandy land grapefruit juice extract of claim 1 is characterized in that may further comprise the steps:
(1) fresh MeiZhou,GuangDong third village shatian pomelo peeling is got shatian pomelo shaddock meat, squeeze juice on juice extractor;
(2) 5000 leave the heart separates grapefruit juice, removes solid residue, obtains the grapefruit juice crude extract;
(3) ethyl acetate of getting 5 times of clear liquid volumes extracts the gained clear liquid, repeat 3 times, combining extraction liquid, decompression concentrates, the sandy land grapefruit juice extract concentrate.
5. the extracting method of the described sandy land grapefruit juice extract of claim 2 is characterized in that may further comprise the steps:
(1) fresh MeiZhou,GuangDong crosses the shatian pomelo peeling in vain, gets shatian pomelo shaddock meat, squeeze juice on juice extractor;
(2) 5000 leave the heart separates grapefruit juice, removes solid residue, obtains the grapefruit juice crude extract;
(3) n-butanol of getting 2 times of clear liquid volumes extracts the gained clear liquid, repeat 3 times, combining extraction liquid, decompression concentrates, the sandy land grapefruit juice extract concentrate.
6. the extracting method of the described sandy land grapefruit juice extract of claim 3 is characterized in that may further comprise the steps:
(1) fresh MeiZhou,GuangDong crosses the shatian pomelo peeling in vain, gets shatian pomelo shaddock meat, squeeze juice on juice extractor;
(2) 5000 leave the heart separates grapefruit juice, removes solid residue, obtains the grapefruit juice crude extract;
(3) ether of getting 5 times of clear liquid volumes extracts the gained clear liquid, repeat 3 times, combining extraction liquid, the water-bath heating concentrates, the sandy land grapefruit juice extract concentrate.
7. the described sandy land grapefruit juice extract of claim 1 has the health food of norcholesterol and effect for reducing blood fat, the application in the beverage in preparation.
8. the described sandy land grapefruit juice extract of claim 2 has the health food of norcholesterol and effect for reducing blood fat, the application in the beverage in preparation.
9. the described sandy land grapefruit juice extract of claim 3 has the health food of norcholesterol and effect for reducing blood fat, the application in the beverage in preparation.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2006100370150A CN1907138B (en) | 2006-08-11 | 2006-08-11 | Sandy land grapefruit juice extract, extraction process and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2006100370150A CN1907138B (en) | 2006-08-11 | 2006-08-11 | Sandy land grapefruit juice extract, extraction process and application thereof |
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| CN1907138A CN1907138A (en) | 2007-02-07 |
| CN1907138B true CN1907138B (en) | 2011-01-12 |
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| CN2006100370150A Expired - Fee Related CN1907138B (en) | 2006-08-11 | 2006-08-11 | Sandy land grapefruit juice extract, extraction process and application thereof |
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| CN107383040B (en) * | 2017-07-05 | 2019-06-07 | 浙江大学 | A method of isolating and purifying bergamot element from early fragrant shaddock oil vacuole layer |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6160006A (en) * | 1996-10-18 | 2000-12-12 | Wayne State University | 6',7'-dihydroxybergamottin, a cytochrome P450 inhibitor in grapefruit |
| CN1528773A (en) * | 2003-09-29 | 2004-09-15 | 中山大学 | Method for extracting shaddock ped aglycone from shaddock |
| CN1726041A (en) * | 2002-12-16 | 2006-01-25 | 贝林格尔·英格海姆国际有限公司 | Use of a combination containing a non-nucleoside reverse transcriptase inhibitor (NNRTI) with an inhibitor of cytochrome p450, such as protease inhibitors |
-
2006
- 2006-08-11 CN CN2006100370150A patent/CN1907138B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6160006A (en) * | 1996-10-18 | 2000-12-12 | Wayne State University | 6',7'-dihydroxybergamottin, a cytochrome P450 inhibitor in grapefruit |
| CN1726041A (en) * | 2002-12-16 | 2006-01-25 | 贝林格尔·英格海姆国际有限公司 | Use of a combination containing a non-nucleoside reverse transcriptase inhibitor (NNRTI) with an inhibitor of cytochrome p450, such as protease inhibitors |
| CN1528773A (en) * | 2003-09-29 | 2004-09-15 | 中山大学 | Method for extracting shaddock ped aglycone from shaddock |
Non-Patent Citations (6)
| Title |
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| T IR ILL IN IB.Grapefruit: the last decadeacquisit ions.[J ]. Fitoterapia71 增111.2000,71(增111),S29- S37. |
| T IR ILL IN IB.Grapefruit: the last decadeacquisit ions.[J ]. Fitoterapia71 增111.2000,71(增111),S29-S37. * |
| 李雪宁等.葡萄柚汁与药物的相互作用.中国临床药理学杂志15 5.1999,15(5),289-293，具体参见摘要、第389页右栏最后一段，第2.2.6节. |
| 李雪宁等.葡萄柚汁与药物的相互作用.中国临床药理学杂志15 5.1999,15(5),289-293,具体参见摘要、第389页右栏最后一段,第2.2.6节. * |
| 睢维国等.柚子提取物对小鼠肝微粒体代谢活性的影响.消化外科4 5.2005,4(5),364-367，具体参见第1.3.2节. |
| 睢维国等.柚子提取物对小鼠肝微粒体代谢活性的影响.消化外科4 5.2005,4(5),364-367,具体参见第1.3.2节. * |
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