CN1903249A - Application of bodinerin for preparing medicine to treat viral influenza and AIDS - Google Patents
Application of bodinerin for preparing medicine to treat viral influenza and AIDS Download PDFInfo
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- CN1903249A CN1903249A CN 200610011095 CN200610011095A CN1903249A CN 1903249 A CN1903249 A CN 1903249A CN 200610011095 CN200610011095 CN 200610011095 CN 200610011095 A CN200610011095 A CN 200610011095A CN 1903249 A CN1903249 A CN 1903249A
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Abstract
An application of the east perilla in preparing the medicines for treating viral inflceenza A and B, and AIDS is disclosed.
Description
Technical field
The present invention relates to a kind of new purposes of medicinal plants East Perillae, be specifically related to the application of East Perillae in preparation treatment epidemic virus flu and HIV (human immunodeficiency virus) medicine, belong to the field of Chinese medicines.
Background technology
East Perillae Elsholtzia bodinieri Van. is a kind of labiate, and it is a perennial herb, and long 10～20 centimetres, rhizome is short, pitchy, the section yellow, fibrous root is many, stem four prismatics, 2～5 millimeters of diameters, surperficial green or purple are by hair, section yellow-white.The leaf multireel contracts, part leaf margin tool knuckle-tooth, and green or aubergine are dredged pubescence, and the back side is close by the gland point.The spike list is born in Zhi Ding, is partial to a side, the corolla aubergine, and close living white becomes mildewed.Gas delicate fragrance, acrid in the mouth, little hardship.Tender leaf can be used for tea.Former recording in " Chinese medicine voluminous dictionary " once reported to have and induced sweat, sending up the lucid YANG, and the effect of removing heat from the liver, but report treatment first type, Influenza B virus are not arranged, more there is not it can be used to prepare the report of the medicine for the treatment of anti-AIDS.
Summary of the invention
The object of the present invention is to provide the application of East Perillae in preparation treatment influenza A virus medicament.
Another object of the present invention provides the application of East Perillae in preparation treatment Influenza B virus medicine.
Another object of the present invention provides the application of East Perillae in preparation treatment AIDS-treating medicine.
East Perillae can be to be that feedstock production becomes medicament with independent East Perillae when the medicine of the above-mentioned treatment various diseases of preparation, also East Perillae can be prepared into compound preparation with other drug.
When generally East Perillae being prepared into the medicine with above-mentioned new purposes, East Perillae extract is prepared into medicament with routine extraction and preparation process individually or in the adding compound recipe.
Here said extraction to East Perillae can be the water extract with East Perillae, as water decoction, and the concentrated solution of decocting liquid, or the spray-dried powder that obtains behind the East Perillae water extract precipitate with ethanol; Also can be East Perillae to be extracted with ethanol or other organic solvents.
The former medicine of East Perillae directly can certainly be prepared into the preparation of folk prescription or compound recipe.
In order to understand essence of the present invention better, will its new purposes in pharmaceutical field be described with the pharmacological testing and the result of East Perillae below.
One, toxicity test
The result shows＜medicine of 5% concentration and the capsule sample of 10 times of human body consumptions do not have tangible toxic action to Embryo Gallus domesticus.Prove that this extract is safe in utilization.
Two, resisiting influenza virus test
Influenza virus selects for use another name for Sichuan Province to prevent-296 strains (H3N2), and cultural method adopts the cultivation in the chick embryo of influenza virus classics, and cultivation results adopts hemagglutination test to identify.The result shows that the resisiting influenza virus effect is fine.
Three, influenza first type virus activity screening
Method of testing: mdck cell is inoculated 96 well culture plates, puts 5%CO
2, cultivated 24 hours for 37 ℃.Mdck cell adds influenza first type virus 10 respectively
-3(60 times of TCID
50), 37 ℃ of absorption hypsokinesis in 2 hours venom of preventing or cure a disease, add different dilution factor medicines respectively.If virus control and cell contrast were cultivated 36 hours for 37 ℃, observed result, record CPE, calculation sample resisiting influenza virus half-inhibition concentration (IC
50).Positive control drug: virazole (RBV), the abundant pharmaceutcal corporation, Ltd of Zhejiang health (lot number 960501).
Test result:
Table 1 sample influenza first type virus activity measurement result
| Sample number into spectrum | TC 50(μg/ml) | To influenza first type | |
| IC 50(μg/ml) | SI | ||
| The East Perillae sample | ＞1000 | 231.12 | 4.33 |
| RBV | ＞1000 | 2.92 | ＞342.46 |
Annotate: TC
50: the poisonous concentration of medicine half; IC
50: medicine is to viral half-inhibition concentration; SI: selection index, SI=TC
50/ IC
50
Conclusion: sample East Perillae sample has influenza first type virus activity.IC
50For: 231.12 μ g/ml
Four, influenza B virus screening active ingredients
Method of testing: mdck cell is inoculated 96 well culture plates, puts 5%CO
2, cultivated 24 hours for 37 ℃.Mdck cell adds influenza B virus 10 respectively
-2.3(10 times of TCID
50), 37 ℃ of absorption hypsokinesis in 2 hours venom of preventing or cure a disease adds different dilution factor medicines respectively.If virus control and cell contrast were cultivated 36 hours for 37 ℃, observed result, record CPE, calculation sample resisiting influenza virus half-inhibition concentration (IC
50).
Positive control drug: virazole (RBV), the abundant pharmaceutcal corporation, Ltd of Zhejiang health (lot number 960501).
Test result:
Table 2 sample influenza B virus determination of activity result
| Sample number into spectrum | TC 50(μg/ml) | IC 50(μg/ml) | SI |
| The East Perillae sample | 1000 | 333.33 | 3.0 |
| RBV | ＞1000 | 3.8 | ＞263.16 |
Conclusion: sample East Perillae sample has influenza B virus activity.IC
50Be 333.33, SI is 3.0
Five, external anti-HIV-1 protease (HIV-1PR) screening active ingredients
Method of testing: add behind the diluted sample and contain in the reaction buffer of fluorescent labeling substrate, and add genetic engineering target enzyme, under optimum reaction condition, hatch, measure fluorescent value with FLUO star Galaxy luminoscope.Positive control drug: indinavir (indinavir), Glaxo Ltd. provides.
Experimental result:
Table 3 sample anti-HIV-1 protease activity determination result
| Sample number into spectrum | 250 (μg/ml) | 50 (μg/ml) | 10 (μg/ml) | 2.0 (μg/ml) | 0.40 (μg/ml) | IC50 (μg/ml) |
| The East Perillae sample | 89.38 | 34.29 | 4.45 | 5.48 | 2.48 | 45.88 |
| 10nM | ||||||
| indinavir | 87.2 |
Conclusion: sample East Perillae sample has the activity of inhibition to HIV-1PR.
Six, the external anti-HIV-1 reverse transcriptase of sample (HIV-1RT) screening active ingredients
Method of testing: add behind the diluted sample and contain among the reaction Buffer of Biotin-dUTP and genetic engineering target enzyme, under optimum reaction condition, hatch,, measure OD with the horseradish peroxidase system colour developing of the plain labelling of affinity
450Value.
Positive control drug: foscarnet sodium (PFA), produce by Changzhou San Chang
Test result:
Table 4 sample anti-HIV-1 reverse transcriptase activity measurement result
| Sample number into spectrum | Initial concentration | IC50 |
| The East Perillae sample | 167μg/ml | 4.7μg/ml |
| PFA | 4μg/ml | 0.165μg/ml |
Conclusion: sample East Perillae sample has the activity of inhibition to the HIV-1RT enzyme, to the IC of HIV-1RT enzymatic activity inhibition
50Be 4.7 μ g/ml.
Seven, the external anti-HIV-1 intergrase of sample (HIV-1IN) screening active ingredients
Method of testing: during adding donor substrate bag is pulled by 96 holes behind the diluted sample, and adding contains among the reaction Buffer of genetic engineering target enzyme and biotin-target substrate, under optimum reaction condition, hatch,, measure 405nm absorption value OD value with biotin labeled alkaline phosphoric acid enzyme system colour developing.
Positive control drug: S-y, the organic institute in Shanghai provides.
Test result:
Table 5 sample anti-HIV-1 intergrase determination of activity result
| Sample number into spectrum | Initial concentration (μ g/ml) | IC 50 (μg/ml) |
| The East Perillae sample | 300 | 6.25 |
| S-y | 0.182 |
Conclusion: sample East Perillae sample has the activity of inhibition, IC to HIV-1 IN enzyme
50Be 6.25 μ g/ml
Eight, bacteriostatic test
Detection method adopts the steel ring method.The result shows has obvious inhibitory action to relevant antibacterials with upper respiratory tract such as Diplococcus pneumoniae, Jia Xingrongxuexinglianqiujun, beta hemolytic streptococcuss.
The invention has the advantages that:
1, the invention provides the extract that the plant East Perillae can be used as medicine components, this extract can use separately, also can mix use with the other plant extract.
2, the present invention has excavated new medical application to the known plants East Perillae, has opened up a new application.
3, East Perillae safety non-toxic of the present invention, pharmacological action is strong, is indicating well prospect in medicine.
4, the medicine that is mixed with of East Perillae of the present invention can be multiple dosage form, as: oral capsule, tablet, powder, pill, granule, oral liquid, soft capsule, unguentum, tincture, powder, injection etc.
The specific embodiment
Embodiment 1:
Get East Perillae 1000 grams, add 5000 ml waters and decoct three times, 3 hours for the first time, 2 hours for the second time, 2 hours for the third time, filter, filtrate concentrates, the dry East Perillae water extract extractum that obtains, and adds general pharmaceutic adjuvant micropowder silica gel, microcrystalline Cellulose, makes tablet according to common technology.Be used for the treatment of first type influenza.
Embodiment 2:
Get East Perillae 300 grams, Herba Senecionis Scandentis 300 grams, the Radix Astragali 400 grams, Rhizoma Et Radix Notopterygii 350 grams, extract three times with 5000 milliliter of 50～70% ethanol hot reflux under 80 ℃ of temperature conditions, filter, decompression filtrate recycling ethanol, concentrate extract extractum, add general pharmaceutic adjuvant Icing Sugar, make granule according to common technology.Be used for the treatment of B-mode influenza.
Embodiment 3:
Get East Perillae 1000 gram, extract three times with 3500 milliliters of ethanol soaking at room temperature, filter, 77 ℃ are reclaimed ethanol, concentrated extract extractum, and last macroporous resin column is got alcohol and is washed part, and concentrate drying must be made with extra care thing; Add general pharmaceutic adjuvant micropowder silica gel, make capsule according to common technology.Be used for the treatment of acquired immune deficiency syndrome (AIDS).
Claims (3)
1. the application of East Perillae in preparation treatment influenza A virus medicament.
2. the application of East Perillae in preparation treatment Influenza B virus medicine.
3. the application of East Perillae in preparation treatment AIDS-treating medicine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610011095 CN1903249A (en) | 2006-08-02 | 2006-08-02 | Application of bodinerin for preparing medicine to treat viral influenza and AIDS |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610011095 CN1903249A (en) | 2006-08-02 | 2006-08-02 | Application of bodinerin for preparing medicine to treat viral influenza and AIDS |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1903249A true CN1903249A (en) | 2007-01-31 |
Family
ID=37672709
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200610011095 Pending CN1903249A (en) | 2006-08-02 | 2006-08-02 | Application of bodinerin for preparing medicine to treat viral influenza and AIDS |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1903249A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102302617A (en) * | 2011-09-07 | 2012-01-04 | 楚雄老拨云堂药业有限公司 | Traditional Chinese medicinal preparation for invigorating stomach, relaxing bowel and reducing blood fat and preparation method thereof |
-
2006
- 2006-08-02 CN CN 200610011095 patent/CN1903249A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102302617A (en) * | 2011-09-07 | 2012-01-04 | 楚雄老拨云堂药业有限公司 | Traditional Chinese medicinal preparation for invigorating stomach, relaxing bowel and reducing blood fat and preparation method thereof |
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