CN1896250A - Production of lycopene by liquid deep-submerged fermentation - Google Patents
Production of lycopene by liquid deep-submerged fermentation Download PDFInfo
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- CN1896250A CN1896250A CN 200610019370 CN200610019370A CN1896250A CN 1896250 A CN1896250 A CN 1896250A CN 200610019370 CN200610019370 CN 200610019370 CN 200610019370 A CN200610019370 A CN 200610019370A CN 1896250 A CN1896250 A CN 1896250A
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Abstract
Production of lycopene by liquid deep fermentation is carried out by culturing strain, physically chemical inducing, forming high-yield strain, culturing seed, fermentation culturing, adding into precursor and antioxidant, adding into inhibitor, filtering, vacuum drying, crushing, extracting by acetone, separating by silica gel column, concentrating and forming object product. It is safe, convenient and cheap and has more output.
Description
Technical field
The present invention relates to a kind of fermentative Production lycopene method of utilizing, more particularly it is a kind of liquid submerged fermentation method production lycopene method.Lyeopene is mainly used in foodstuff additive and health products trade.
Background technology
The Lyeopene that is contained in the tomato is a natural pigment, to human body safety, and because it has many physiological activities to human body, food, medicine, makeup, etc. application prospects in the field, so the extraction production of Lyeopene and application receive great concern.
Existing production lycopene method generally is to extract to produce from tomato, tomato skin, tomato-sauce, tomato puree or watermelon.
Main method is as follows:
1, solvent method, general step is as follows: the pre-treatment of a. raw material: raw materials pretreatment is extremely important for the extraction of Lyeopene, general pretreatment technology is that raw material is after water treatment, with most of Impurity removal, drying, the alcohol with 95% is handled, sometimes also can add antioxidants such as BHT, extract again.B. extract choice of Solvent: normal hexane, hexanaphthene, sherwood oil, acetone, ethyl acetate are generally determining of extraction agent c. optimum extraction process commonly used: by the design of orthogonal experiment, determine best extraction conditions, as the optimum extraction agent, extract temperature, extraction time, the pH value, material ratio, the consumption of extraction agent and the ratio of double solvents etc.
Extracting the crude product that obtains through solvent method and be called Lyeopene, is a kind of oil-soluble mixture, wherein except that containing Lyeopene, also contains some amount grease and lipid acid, vitamin-E, lipoid compositions such as sterol, phosphatide.The shortcoming of this method is apparent in view, and promptly the content of Lyeopene is lower in the oleo-resinous, how the Lyeopene of low levels is handled the high purity Lyeopene that obtains according with the demands of the market, and needing further, experiment solves.Dissolvent residual need remove dissolvent residual than higher in the oleo-resinous simultaneously, just can obtain colory Lyeopene.
Pertinent literature: Xinjiang Shengminghong Science and Technology Investment and Development Co., Lt, tomato-sauce is produced crystalline lycopene and/or lycopene method, number of patent application: 00128226.3
2, enzyme process, step is as follows: fresh tomato → remove the peel → smash to pieces → enzyme-added → constant temperature preservation → organic solvent lixiviate → extracting solution → filtration → concentrate → thick product → purified product.
Generally select cellulase, polygalacturonase for use, adopt orthogonal experiment, determine the consumption and the ratio of the optimum process condition such as the enzyme of single enzyme and prozyme, action time, extraction time etc.
This method has shortened extraction time with respect to traditional solvent, but it is higher simultaneously also to exist cost, the effect instability of enzyme, and shortcoming such as yield rate is low is difficult for carrying out the deficiency of suitability for industrialized production.
Pertinent literature: Puruifa Natural Medicine Modern Purificatino and Separation Inst. Co., Ltd, Beij " from tomato-sauce, extracting the high purity lycopene method ", the patent No.: 02145914.2 with biological enzyme
3, supercritical CO
2Extraction process, step is as follows: raw material → making beating → squeeze and filter → vacuum lyophilization → pulverize → sieve → weigh → adorn extraction tank, suitable working parameter → static state, the dynamic extraction → step-down of sealing → control separate → by separator column acquisition Lyeopene → purity testing
General by experiment of single factor, orthogonal experiment and confirmatory experiment determine that optimum process condition is as entrainment agent, raw material particle size, extracting pressure, extraction temperature, extraction time, solvent flow rate etc.
This method have the traditional extraction method incomparable superiority, its great advantage is to cause the consumption of chemical solvents and residual, do not pollute, the extraction conditions gentleness, thereby avoided active substance that oxidative degradation at high temperature takes place, but because this method is very high to the experimental installation requirement, complicated operation, cost is higher, so be difficult to be widely used in suitability for industrialized production.
4, saponification method, step is as follows: fresh tomato → fragmentation → centrifugal → alkaline saponification → washing → ethanol dehydration → drying → pulverizing → sherwood oil lixiviate → vat liquor vacuum rotation concentrates → Lyeopene saponification extract.
By experiment of single factor and orthogonal experiment, determined that the saponified optimum process condition is as saponifying agent, saponification temperature, saponification time etc.Studies show that saponification can effectively remove most of glycerin fatty acid ester and various free fatty acids in the tomato, discharge Lyeopene wherein, can obviously improve the degree of crystallinity and the purity of Lyeopene, but the shortcoming of saponification method also clearly, cost increases, process complications also exists the problem that dissolvent residual is difficult for removal simultaneously.
5, the microwave extraction, step is as follows: fresh tomato → smash to pieces → microwave → organic solvent lixiviate → extracting solution → filtration → filtrate → concentrate → thick product → purified product.
By investigation, determine that optimum process condition is as microwave power, extraction agent, extraction time, pH, liquid-solid ratio etc. to each factor.
The microwave lixiviate is a kind of new extractive technique, has that power consumption is low, the operating time is short, solvent consumption is few, selectivity is high, target components yield advantages of higher, but equally also exists significant disadvantages, and promptly the equipment requirements height is difficult for being applied to industrialization, poor stability etc.
Summary of the invention
The objective of the invention is to overcome above-mentioned the deficiencies in the prior art part, and a kind of liquid submerged fermentation method production lycopene method is provided.The inventive method is suitable for industrial fermentation production and production cost is low, easy-to-operate, productive rate height.
The objective of the invention is to reach by following measure: liquid submerged fermentation method production lycopene method is characterized in that it comprises the steps:
A), the trispore Bruce mould bacterial strain that will be numbered 14059 (+), 14060 (-) receives the activation medium activation, activation medium is a slant medium;
B), carry out ultraviolet mutagenesis or nitrosoguanidine mutagenesis when a) cultivating spore germination;
C), with b) obtain the strain excellent of high yield by screening, be inoculated in activation medium;
D), under aseptic condition, from (+) (-) slant medium difference picking two ring spores access seed culture mediums of cultivating 6d; At 27 ℃, under the condition of 150rpm, cultivate 28h;
E), with d) a positive and negative bacterium of growth mixes under aseptic condition at 1: 10, inserts fermention medium with 10% inoculum size again, liquid amount 45ml/250ml, pH 6.5; At 28 ℃, under the condition of 250rpm, cultivate 120h;
F), with e) during fermentation culture 48h, add precursor β-ionone, vazadrine and succinimide, three's addition is respectively 1ml/L, 1g/L, 5g/L; Add the antioxidant 2,6 ditertiary butyl p cresol simultaneously, addition is 1g/L;
G), cultivate finish after, the bacterium liquid after the fermentation is adopted 4-6 layer filtered through gauze, obtain the scarlet thalline; Again with the vacuum-drying of gained thalline, 45 ℃ of temperature, 0.1Mpa, vacuum-drying 24h, the weighing dry cell weight, it is standby that thalline is ground into 60-80 purpose bacterium powder;
H), repeatedly extract as extraction agent, solid-liquid ratio is 1: 3, and extraction temperature is 30-40 ℃, and the extraction time is 1.5-3h, and pH is 5.5-8.0, adds 0.5% antioxidant 2,6 ditertiary butyl p cresol in the extraction process with acetone;
After the gradation extraction, collect extraction liquid, by column chromatography separating lycopene and other pigments, sorbent material is a 100-200 order chromatographic silica gel, and eluent is an acetone, and Lyeopene and acetone ratio are 1: 10, and eluting temperature is a room temperature, and flow velocity is 3.5ml/min;
I), gained elutriant heating, concentrate, promptly get target product 6% Lyeopene.
In technique scheme, described activation culture based component is: potato extractive juice 1L, glucose 20g/L, KH
2PO
43g/L, MgSO
47H
2O 1.5g/L, agar 15g/L, VB
1Trace.
In technique scheme, described ultraviolet mutagenesis method is: preparation bacteria suspension, the centrifugal substratum of going out, prepare bacteria suspension with physiological saline, add the granulated glass sphere concussion and disperse, filter with aseptic filter paper or absorbent cotton, make formation unicellular, degree of scatter reaches 90%-95%, and bacteria suspension concentration is about 10
6-10
7Ml
-1Carry out uviolizing again, get the above bacteria suspension 5ml for preparing in diameter 9cm plate, ultraviolet lamp is opened preheating 20min, to stablize light wave; The plate that fills bacteria suspension is put on the magnetic stirring apparatus, opens the ware lid, expose under the UV-light and shine 5-10min, irradiation makes cell evenly absorb ultraviolet light wave while stirring; Postradiation thalline is changed in the sterile test tube, and immerse 1-2h in the frozen water; The bacteria suspension that irradiation finishes joins in the substratum that is suitable for gain mutant propagation, cultivates 1.5-2h under optimal temperature, and adds vazadrine etc. in proliferated culture medium; After cultivate to finish the back, therefrom get a certain amount of culture, make different extent of dilution, be coated with ware, and compare ware, after the cultivation, choose bacterium colony, screen waiting with the bacteria suspension that non-irradiated with ultraviolet radiation is crossed.
In technique scheme, described nitrosoguanidine mutagenesis: through centrifugal, washing, make suspension with damping fluid, concentration is about 10
6-10
7Ml
-1Preparation nitrosoguanidine mother liquor (1-methyl-3-nitro-1-nitrosoguanidine) adds solubility promoter methane amide or acetone 10-50 μ l/ml, adds buffered soln then, and its ratio is 9: 1, and the nitrosoguanidine mother liquid concentration is made into 10mg/ml, and concentration of treatment is 1-3mg/ml; Above bacteria suspension and nitroso guanidine solution are contained in one in vitro, put 28 ℃ and handle about 90-120min; Be diluted to more than 50 times or carry out centrifuge washing at low temperatures with cold physiological saline, remove soup, adding sterilized water, to make precipitation suspend and make extent of dilution be 10
3-10
6Ml
-1, be located away from plate; Nitrosoguanidine, agar and thalline are mixed and made into flat board, and nitrosoguanidine concentration is 10-50 μ g/ml; Or nutrient agar made flat board, and nitrosoguanidine and thalline to be mixed smear flat board then, this moment, nitrosoguanidine concentration was 10-20 μ g/ml.
In technique scheme, described seed culture based component is: glucose 10g/L, W-Gum 30g/L, corn steep liquor 60g/L, potassium primary phosphate 0.5g/L, sal epsom 0.25g/L, VB
10.5mg/L.
In technique scheme, described fermentation culture based component is: W-Gum 40g/L, corn steep liquor 20g/L, soybean cake powder 20g/L, Oleum Gossypii semen 50ml/L, potassium primary phosphate 1g/L, sal epsom 0.25g/L, VB0.5mg/L.
In technique scheme, at h) in, described extraction temperature is 35 ℃, the extraction time is 2h, pH is 6.5.
Liquid submerged fermentation method production lycopene method of the present invention has following advantage:
1. the present invention is that trispore Bruce mould liquid submerged fermentation method is produced Lyeopene, extracts Lyeopene in the starting material such as farm crop and compares with traditional, have with low cost, the output height, simple to operate, be easy to advantages such as industrialization;
2. the present invention had done physical chemistry mutagenesis to starting strain before this, had obtained the strain excellent of high yield, had improved the productive rate of Lyeopene further, for the suitability for industrialized production of product lays a solid foundation;
3. (three's addition is respectively 1ml/L for β-ionone, vazadrine and succinimide to add the prerequisite thing in the fermentation of the present invention, 1g/L, 5g/L) and antioxidant (BHT, 1g/L), improve the output of target product greatly, and effectively prevented the degraded of Lyeopene;
4. the blocker of the present invention's employing is the tobacco waste (derivative that contains the 3-pyridyl of 0.5%-12% in the tobacco waste, wherein nicotine accounts for 95%), traditional blocker toxicity is big, the cost height, and the blocking-up rate is unsatisfactory, and the tobacco waste wide material sources, with low cost, it is convenient, safe to use, and the material that can effectively increase thalline inside passes oxygen, has improved the productive rate of Lyeopene;
5. acetone extract-silicagel column separation purification method of adopting of the present invention, solved low (traditional method for extracting rate＜85% of traditional solvent extraction yield effectively, and present method extraction yield 〉=95%), (traditional method adopts sherwood oil or cyclohexane extraction to dissolvent residual more, dissolvent residual is difficult for removing, present method adopts acetone as extraction agent, the extraction yield height, volatility is little, self property is stable, be easy to reclaim, heating back no solvent residue, dissolving pigment ability is strong) and other pigment interferential problems (silicagel column is separating lycopene and other carotene effectively), and convenient and easy, cost is lower;
6. the measuring method with the Sudan I replacement Pure Lycopene of the present invention's employing is simple and convenient, the accuracy rate height, and rate of recovery height, good reproducibility, and effectively avoided the standard substance difficult problem of degraded fast, greatly reduce cost;
7. the target product of gained of the present invention is 6% Lyeopene, compares with existing like product on the market, and it is higher to have a content of lycopene, advantage such as foreign matter content is few, and production cost is low.
Description of drawings
Fig. 1 is the process flow sheet of liquid submerged fermentation method production lycopene method of the present invention.
Embodiment
Describe the performance of liquid submerged fermentation method production lycopene method of the present invention in detail below in conjunction with accompanying drawing:
Embodiment 1
The technical process of liquid submerged fermentation method production lycopene method of the present invention is:
1., (introduce from ATCC, numbering is respectively 14059 (+), 14060 (-) receive the activation medium activation, and activation medium is a slant medium: potato extractive juice 1L, glucose 20g/L, KH with the trispore Bruce mould bacterial strain of CCTCC
2PO
43g/L, MgSO
4H
2O 1.5g/L, agar 15g/L, VB
1Trace;
2., cultivate and carry out physical chemistry mutagenesis when most spores have just been sprouted.
The ultraviolet mutagenesis method is: prepare bacteria suspension, the centrifugal substratum of going out prepares bacteria suspension with physiological saline, adds the granulated glass sphere concussion and disperses, and filters with aseptic filter paper or absorbent cotton, makes formation unicellular, and degree of scatter reaches 90%-95%, and bacteria suspension concentration is about 10
6-10
7Ml
-1Carry out uviolizing again, get the above bacteria suspension 5ml for preparing in diameter 9cm plate, ultraviolet lamp is opened preheating 20min, to stablize light wave; The plate that fills bacteria suspension is put on the magnetic stirring apparatus, opens the ware lid, expose under the UV-light and shine 5-10min, irradiation makes cell evenly absorb ultraviolet light wave while stirring; Postradiation thalline is changed in the sterile test tube, and immerse 1-2h in the frozen water; The bacteria suspension that irradiation finishes joins in the substratum that is suitable for gain mutant propagation, cultivates 1.5-2h under optimal temperature, and adds vazadrine etc. in proliferated culture medium; After cultivate to finish the back, therefrom get a certain amount of culture, make different extent of dilution, be coated with ware, and compare ware, after the cultivation, choose bacterium colony, screen waiting with the bacteria suspension that non-irradiated with ultraviolet radiation is crossed.
3., obtain the strain excellent of high yield, be inoculated in activation medium, medium component is constant by screening;
4., under aseptic condition, from (+) (-) slant medium difference picking two ring spores access seed culture of cultivating 6d.At 27 ℃, under the condition of 150rpm, cultivate 28h.Seed culture medium is: glucose 10g/L, W-Gum 30g/L, corn steep liquor 60g/L, potassium primary phosphate 0.5g/L, sal epsom 0.25g/L, VB
10.5mg/L;
5., eugonic positive and negative bacterium is mixed under aseptic condition at 1: 10, insert fermention medium with 10% inoculum size again, liquid amount 45ml/250ml, pH 6.5.At 28 ℃, under the condition of 250rpm, cultivate 120h; Fermention medium: W-Gum 40g/L, corn steep liquor 20g/L, soybean cake powder 20g/L, Oleum Gossypii semen 50ml/L, potassium primary phosphate 1g/L, sal epsom 0.25g/L, VB, 0.5mg/L.
6., when fermentation culture 48h, add precursor β-ionone, vazadrine and succinimide, three's addition is respectively 1ml/L, 1g/L, 5g/L.
7., simultaneously add antioxidant BHT, addition is 1g/L.
8., cultivate finish after, the bacterium liquid after the fermentation is adopted 4-6 layer filtered through gauze, obtain the scarlet thalline; Again with the vacuum-drying of gained thalline (45 ℃, under the 0.1Mp vacuum), vacuum-drying 24h, the weighing dry cell weight, it is standby that thalline is ground into 60-80 purpose bacterium powder.
9., repeatedly extract as extraction agent, solid-liquid ratio is 1: 3, and extraction temperature is 30 ℃, and the extraction time is 1.5h, and pH is 5.5 with acetone.Add 0.5% antioxidant BHT (2,6 ditertiary butyl p cresol) in the extraction process, extraction yield can reach 96%.
After the gradation extraction, collect extraction liquid, by column chromatography separating lycopene and other pigments, concrete parameter is as follows: sorbent material is a 100-200 order chromatographic silica gel, and eluent is an acetone, and Lyeopene and acetone ratio are 1: 10, eluting temperature is a room temperature, and flow velocity is 3.5ml/min.
10., gained elutriant heating, concentrate, promptly get target product 6% Lyeopene.
Embodiment 2
Other step is with embodiment 1.
2., cultivate and carry out physical chemistry mutagenesis when most spores have just been sprouted.
The nitrosoguanidine mutagenesis method is: through centrifugal, washing, make suspension with damping fluid, concentration is about 10
6-10
7Ml
-1Preparation nitrosoguanidine mother liquor (1-methyl-3-nitro-1-nitrosoguanidine) adds solubility promoter methane amide or acetone 10-50 μ l/ml, adds buffered soln then, and its ratio is 9: 1, and the nitrosoguanidine mother liquid concentration is made into 10mg/ml, and concentration of treatment is 1-3mg/ml; Above bacteria suspension and nitroso guanidine solution are contained in one in vitro, put 28 ℃ and handle about 90-120min; Be diluted to more than 50 times or carry out centrifuge washing at low temperatures with cold physiological saline, remove soup, adding sterilized water, to make precipitation suspend and make extent of dilution be 10
3-10
6Ml
-1, be located away from plate; Nitrosoguanidine, agar and thalline are mixed and made into flat board, and nitrosoguanidine concentration is 10-50 μ g/ml; Or nutrient agar made flat board, and nitrosoguanidine and thalline to be mixed smear flat board then, this moment, nitrosoguanidine concentration was 10-20 μ g/ml.
9., repeatedly extract as extraction agent, solid-liquid ratio is 1: 3, and extraction temperature is 40 ℃, and the extraction time is 3h, and pH is 8.0 with acetone.Add 0.5% antioxidant BHT (2,6 ditertiary butyl p cresol) in the extraction process, extraction yield can reach 96%.
Embodiment 3
Other step is with embodiment 1.
9., repeatedly extract as extraction agent, solid-liquid ratio is 1: 3, and extraction temperature is that temperature is 35 ℃, and the time is 2h, and pH is 6.5 with acetone.Add 0.5% antioxidant BHT (2,6 ditertiary butyl p cresol) in the extraction process, extraction yield can reach 96%.
Need to prove that for these professional those skilled in the art under the situation that does not change the principle of the invention, can also make suitable change and distortion to the present invention, this belongs to protection scope of the present invention equally.
Claims (7)
1, liquid submerged fermentation method production lycopene method is characterized in that it comprises the steps:
A), trispore Bruce mould bacterial strain that CCTCC is numbered 14059 (+), 14060 (-) receives the activation medium activation, activation medium is a slant medium;
B), carry out ultraviolet mutagenesis or nitrosoguanidine mutagenesis when a) cultivating spore germination;
C), with b) obtain the strain excellent of high yield by screening, be inoculated in activation medium;
D), under aseptic condition, from (+) (-) slant medium difference picking two ring spores access seed culture mediums of oneself cultivation 6d; At 27 ℃, under the condition of 150rpm, cultivate 28h;
E), with d) a positive and negative bacterium of growth mixes under aseptic condition at 1: 10, again with 10% insert a fermention medium by kind of an amount, liquid amount 45ml/250ml, pH 6.5; At 28 ℃, under the condition of 250rpm, cultivate 120h;
F), with e) during fermentation culture 48h, add precursor β-ionone, vazadrine and succinimide, three's addition is respectively 1ml/L, 1g/L, 5g/L; Add the antioxidant 2,6 ditertiary butyl p cresol simultaneously, addition is 1g/L;
G), cultivate finish after, the bacterium liquid after the fermentation is adopted 4-6 layer filtered through gauze, obtain the scarlet thalline; Again with the vacuum-drying of gained thalline, 45 ℃ of temperature, 0.1Mpa, vacuum-drying 24h, the weighing dry cell weight, it is standby that thalline is ground into 60-80 purpose bacterium powder;
H), repeatedly extract as extraction agent, solid-liquid ratio is 1: 3, and extraction temperature is 30-40 ℃, and the extraction time is 1.5-3h, and pH is 5.5-8.0, adds 0.5% antioxidant 2,6 ditertiary butyl p cresol in the extraction process with acetone;
After the gradation extraction, collect extraction liquid, by column chromatography separating lycopene and other pigments, sorbent material is a 100-200 order chromatographic silica gel, and eluent is an acetone, and lycopene oleo-resinous and acetone ratio are 1: 10, eluting temperature is a room temperature, and flow velocity is 3.5ml/min;
I), gained elutriant heating, concentrate, promptly get target product 6% lycopene oleo-resinous.
2, liquid submerged fermentation method production lycopene method according to claim 1 is characterized in that described activation culture based component is: potato extractive juice 1L, glucose 20g/L, KH
2PO
43g/L, MgSO
47H
2O 1.5g/L, agar 15g/L, VB
1Trace.
3, liquid submerged fermentation method production lycopene method according to claim 1, it is characterized in that described ultraviolet mutagenesis method is: the preparation bacteria suspension, the centrifugal substratum of going out, prepare bacteria suspension with physiological saline, add the granulated glass sphere concussion and disperse, filter, make formation unicellular with aseptic filter paper or absorbent cotton, degree of scatter reaches 90%-95%, and bacteria suspension concentration is about 10
6-10
7Ml
-1Carry out uviolizing again, get the above bacteria suspension 5ml for preparing in diameter 9cm plate, ultraviolet lamp is opened preheating 20min, to stablize light wave; The plate that fills bacteria suspension is put on the magnetic stirring apparatus, opens the ware lid, expose under the UV-light and shine 5-10min, irradiation makes cell evenly absorb ultraviolet light wave while stirring; Postradiation thalline is changed in the sterile test tube, and immerse 1-2h in the frozen water immediately; The bacteria suspension that irradiation finishes joins in the substratum that is suitable for gain mutant propagation, cultivates 1.5-2h under optimal temperature, and adds vazadrine etc. in proliferated culture medium; After cultivate to finish the back, therefrom get a certain amount of culture, make different extent of dilution, be coated with ware, and compare ware, after the cultivation, choose bacterium colony, screen waiting with the bacteria suspension that non-irradiated with ultraviolet radiation is crossed.
4, liquid submerged fermentation method production lycopene method according to claim 1 is characterized in that described nitrosoguanidine mutagenesis: through centrifugal, washing, make suspension with damping fluid, concentration is about 10
6-10
7Ml
-1Preparation nitrosoguanidine mother liquor adds solubility promoter methane amide or acetone 10-50 μ l/ml, adds buffered soln then, and its ratio is 9: 1, and the nitrosoguanidine mother liquid concentration is made into 10mg/ml, and concentration of treatment is 1-3mg/ml; Above bacteria suspension and nitroso guanidine solution are contained in one in vitro, put 28 ℃ and handle about 90-120min; Be diluted to more than 50 times or carry out centrifuge washing at low temperatures with cold physiological saline, remove soup, adding sterilized water, to make precipitation suspend and make extent of dilution be 10
3-10
6Ml
-1, be located away from plate; Nitrosoguanidine, agar and thalline are mixed and made into flat board, and nitrosoguanidine concentration is 10-50 μ g/ml; Or nutrient agar made flat board, and nitrosoguanidine and thalline to be mixed smear flat board then, this moment, nitrosoguanidine concentration was 10-20 μ g/ml.
5, liquid submerged fermentation method production lycopene method according to claim 1 is characterized in that described seed culture based component is: glucose 10g/L, W-Gum 30g/L, corn steep liquor 60g/L, potassium primary phosphate 0.5g/L, sal epsom 0.25g/L, VB
10.5mg/L.
6, liquid submerged fermentation method production lycopene method according to claim 1, it is characterized in that described fermentation culture based component is: W-Gum 40g/L, corn steep liquor 20g/L, soybean cake powder 20g/L, Oleum Gossypii semen 50ml/L, potassium primary phosphate 1g/L, sal epsom 0.25g/L, VB0.5mg/L.
7, liquid submerged fermentation method production lycopene method according to claim 1 is characterized in that at h) in, described extraction temperature is 35 ℃, the extraction time is 2h, pH is 6.5.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610019370 CN1896250A (en) | 2006-06-15 | 2006-06-15 | Production of lycopene by liquid deep-submerged fermentation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610019370 CN1896250A (en) | 2006-06-15 | 2006-06-15 | Production of lycopene by liquid deep-submerged fermentation |
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| Publication Number | Publication Date |
|---|---|
| CN1896250A true CN1896250A (en) | 2007-01-17 |
Family
ID=37608896
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101987809A (en) * | 2009-08-05 | 2011-03-23 | 甘肃省格瑞斯生物科技有限公司 | Production technology for extracting purified lycopene from tomato waste residue |
| CN110283854A (en) * | 2019-08-08 | 2019-09-27 | 内蒙古金达威药业有限公司 | A kind of fermentation medium and its application and the method for preparing lycopene using Blakeslea trispora fermentation |
-
2006
- 2006-06-15 CN CN 200610019370 patent/CN1896250A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101987809A (en) * | 2009-08-05 | 2011-03-23 | 甘肃省格瑞斯生物科技有限公司 | Production technology for extracting purified lycopene from tomato waste residue |
| CN110283854A (en) * | 2019-08-08 | 2019-09-27 | 内蒙古金达威药业有限公司 | A kind of fermentation medium and its application and the method for preparing lycopene using Blakeslea trispora fermentation |
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