CN109134414A - A method of purple sweet potato anthocyanin is separated with glutaraldehyde modified magnetic chitosan nano particle - Google Patents
A method of purple sweet potato anthocyanin is separated with glutaraldehyde modified magnetic chitosan nano particle Download PDFInfo
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- CN109134414A CN109134414A CN201811009277.5A CN201811009277A CN109134414A CN 109134414 A CN109134414 A CN 109134414A CN 201811009277 A CN201811009277 A CN 201811009277A CN 109134414 A CN109134414 A CN 109134414A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/58—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4
- C07D311/60—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2
- C07D311/62—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2 with oxygen atoms directly attached in position 3, e.g. anthocyanidins
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
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- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
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- B01J20/28004—Sorbent size or size distribution, e.g. particle size
- B01J20/28007—Sorbent size or size distribution, e.g. particle size with size in the range 1-100 nanometers, e.g. nanosized particles, nanofibers, nanotubes, nanowires or the like
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- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
- B01J20/28002—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their physical properties
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Abstract
A method of purple sweet potato anthocyanin is separated with glutaraldehyde modified magnetic chitosan nano particle, the purple sweet potato juice rich in anthocyanidin is prepared by raw material of purple sweet potato, adsorbing separation is carried out to the anthocyanidin in purple sweet potato juice using the modified chitosan magnetic nano particle of glutaraldehyde as adsorption and separation material again, finally obtains purple sweet potato anthocyanin product by elution, vacuum concentration and freeze-drying.A kind of method with glutaraldehyde modified magnetic chitosan nano particle separation purple sweet potato anthocyanin of the invention, simple process is easy to operate, and easy to promote and utilize, low energy consumption for separation process, no hazardous waste products, and comprehensive resource utilization rate is high.A kind of method with glutaraldehyde modified magnetic chitosan nano particle separation purple sweet potato anthocyanin of the invention, prepared purple sweet potato anthocyanin purity is high, can be widely applied to health care product, in food service industry as antioxidant, natural pigment and nutrition fortifier etc..
Description
Technical field
The invention belongs to food processing technology field, it is related to the preparation method of purple sweet potato anthocyanin more particularly to a kind of uses penta
The method of dialdehyde chitosan magnetic nano particle separation purple sweet potato anthocyanin.
Background technique
Anthocyanidin (Anthocyanidin) belongs to as one of nature water-soluble natural pigment is widely present in
The compound of flavonoid class, the anthocyanidin in purple sweet potato is relatively stable, is also concerned in recent years.It has non-as natural pigment
Often good anti-aging effects；It can promote blood circulation；Eyesight, the fatigue appropriate for alleviating eyes can be enhanced；To human body
Cardio-pulmonary function also has certain promotion protective effect, can also prevent senile dementia etc..
Currently, in daily life human intake's anthocyanidin mainly by direct diet, and separation and Extraction anthocyanidin
The cost is relatively high and complex steps, how high efficiency, inexpensive extraction anthocyanidin become research emphasis.Magnetic Nano
Grain not only has that surface area itself is big after the surface modification of chitosan, magnetic good feature, and after modification
Agglomeration is effectively prevented, good biocompatibility is shown.The present invention uses chitosan magnetic surface area greatly, more
The characteristics of being easy to separate and recover recycling prepares the purple sweet potato juice rich in anthocyanidin by raw material of purple sweet potato, then with glutaraldehyde
Modified chitosan magnetic nano particle is that adsorption and separation material carries out adsorbing separation to the anthocyanidin in purple sweet potato juice, is finally passed through
Elution is concentrated in vacuo and is lyophilized and obtains purple sweet potato anthocyanin product.It is easy to operate with simple process, it is energy saving, it is no harmful useless
The features such as object discharge, comprehensive resource utilization rate is high, and product purity is high and easy to promote and utilize.It is prepared using the method for the present invention
Product, can be widely applied to health care product, in food service industry as antioxidant, natural pigment and nutrition fortifier etc..
Summary of the invention
Purple sweet potato anthocyanin is separated with glutaraldehyde modified magnetic chitosan nano particle the purpose of the present invention is to provide a kind of
Method, it is big according to chitosan magnetic surface area, be easier the characteristics of separating and recovering, with the modified chitosan magnetic of glutaraldehyde
Nano particle is that adsorption and separation material carries out adsorbing separation to the anthocyanidin in purple sweet potato juice.
For achieving the above object, technical scheme is as follows:
A method of purple sweet potato anthocyanin is separated with glutaraldehyde modified magnetic chitosan nano particle, comprising the following steps:
S1: purple sweet potato juice and the glutaraldehyde modified magnetic chitosan nano particle that pH is 3~5 are prepared respectively；
S2: glutaraldehyde modified magnetic chitosan nano particle is placed in the purple sweet potato juice that pH is 3~5, under the conditions of earthquake
Carry out saturation absorption；
S3: in the glutaraldehyde modified magnetic chitosan nano for isolating saturation absorption purple sweet potato anthocyanin outside plus under magnetic field condition
Particle；
S4: by isolate saturation absorption purple sweet potato anthocyanin glutaraldehyde modified magnetic chitosan nano particle be placed in containing
It is ultrasonically treated in 55~65 DEG C of organic solvent of hydrochloric acid, obtains purple sweet potato anthocyanin eluent；
S5: purple sweet potato anthocyanin eluent is concentrated in vacuo, is freeze-dried to obtain purple sweet potato anthocyanin freeze-dried powder.
As currently preferred technical solution, in the step S1 preparing pH be 3~5 purple sweet potato juice comprising steps of
A1 it) will be crushed after the cleaning of light violet potato, water and Thermostable α-Amylase, enzymatic hydrolysis 1.5 be added in broken purple sweet potato
Purple sweet potato slurry is obtained after~2 hours；
A2) squeezing purple sweet potato starches to obtain purple sweet potato juice；
A3 carbohydrase is added after) adjusting the pH of purple sweet potato juice, carries out carbohydrase under conditions of hydrolysis temperature is 50-55 DEG C
Solution；
A4 it) adjusts after saccharification digests and pectase is added after the pH of purple sweet potato juice, carry out enzyme under conditions of hydrolysis temperature is 30
Solution；
A5) sequentially add bentonite, active carbon is stood after stirring, filtering, using hydrochloric acid deploy pH be 3~5 purple sweet potato
Juice；
As currently preferred technical solution, glutaraldehyde modified magnetic chitosan nano is prepared in the step S1
Grain comprising steps of
B1) by FeCl3·6H2O and FeCl2·4H2O be added nitrogen protection water in, stirring, adjust pH to 10~11,80
After DEG C water-bath, in separation product outside plus under magnetic field condition, and Fe is obtained to neutrality with the abundant washed product of distilled water3O4Magnetic core is molten
Liquid；
B2 the acetic acid solution for) preparing chitosan-containing, by Fe3O4Magnetic core solution, acetic acid solution, hexamethylene and n-hexyl alcohol mixing
Lotion is obtained afterwards, and Triton X-100 is slowly added into gained lotion to lotion from black becomes white, continuously adds Triton
X-100 gradually becomes black to lotion, and NaOH, water-bath is added, and dehydrated alcohol washs respectively with water, obtains chitosan magnetic nanometer
Grain solution；
B3) by the chitosan magnetic nanoparticles solution constant volume of acquisition in Fe3O4Concentration shakes activation, water in glutaraldehyde
It washes, obtains glutaraldehyde modified magnetic chitosan nano particle.
As currently preferred technical solution, the step b1) it specifically includes: it is added into the water of nitrogen protection
FeCl3·6H2O and FeCl2·4H2O, and make F3+With Fe2+Molar ratio is 2:1, stirs 5~10 minutes, makes FeCl3·6H2O and
FeCl2·4H2O is completely dissolved in water, be added dropwise into the aqueous solution after stabilization concentrated ammonia liquor to pH be 10~11, later 80
Products therefrom is separated outside plus under magnetic field condition, and is washed with distilled water to neutrality by water-bath 30min in DEG C, obtains Fe3O4Magnetic core
Solution.
As currently preferred technical solution, the Fe3O4Magnetic core solution, acetic acid solution, hexamethylene and n-hexyl alcohol body
Product is than being 4:4:11:6.
As currently preferred technical solution, the step b3) it specifically includes: by the chitosan magnetic nanometer of acquisition
Grain constant volume is in 10mg/mL Fe3O4Concentration, in 0.5mol/L glutaraldehyde environment after 150rpm revolving speed concussion activation 60min, washing
2-3 times, obtain glutaraldehyde modified magnetic chitosan nano particle.
As currently preferred technical solution, the step S2 is specifically included: by glutaraldehyde modified magnetic chitosan nano
Rice grain is placed in the purple sweet potato juice that pH is 3~5, and 150rpm shakes the saturation absorption of completion in 1 hour.
As currently preferred technical solution, the step S4 is specifically included: by the saturation isolated absorption purple sweet potato flower
The glutaraldehyde modified magnetic chitosan nano particle of green element is placed in 55~65 DEG C of the methanol solution or ethanol solution containing hydrochloric acid
In, wherein concentration of hydrochloric acid is 0.1mol/L, handles 30~45min in the case where power is the ultrasound environments of 150~250W, obtains purple sweet potato flower
Green element eluent.
As currently preferred technical solution, the methanol solution or ethanol solution are the first that volume ratio is 85~95%
The ethanol solution that alcoholic solution or volume ratio are 65~75%.
As currently preferred technical solution, the step S5 is specifically included: purple sweet potato anthocyanin eluent is pumped into very
In empty enrichment facility, it is concentrated in vacuo under conditions of vacuum degree is -0.6~-0.9Mpa, temperature is 75~85 DEG C, until
In concentrate without methanol or ethyl alcohol until；Purple sweet potato anthocyanin concentrate and evaporation liquid are collected, the purple sweet potato anthocyanin of collection is concentrated
Liquid pre-freeze 5~6 hours in the environment of -18 DEG C freeze under conditions of temperature is -50 DEG C, vacuum degree is 25~50Pa later
It is 24~30 hours dry, obtain the purple sweet potato anthocyanin freeze-dried powder that purity is 95~98%.
Compared with prior art, beneficial effects of the present invention: of the invention is a kind of with glutaraldehyde modified magnetic chitosan nano
The method that rice grain separates purple sweet potato anthocyanin, simple process is easy to operate, easy to promote and utilize；Low, the no nocuousness of separation process energy consumption
Waste discharge, comprehensive resource utilization rate are high；Of the invention is a kind of with glutaraldehyde modified magnetic chitosan nano particle separation purple sweet potato
The method of anthocyanidin, prepared purple sweet potato anthocyanin purity is high, can be widely applied to health care product, in food service industry as anti-oxidant
Agent, natural pigment and nutrition fortifier etc..
Specific embodiment:
Embodiment 1
1. the preparation of purple sweet potato juice is concentrated:
Cleaned using fresh purple sweet potato as raw material, crushing and beating, liquefaction, juicing, secondary juice are saccharified, clarify, canned standby
With.Specific operating procedure are as follows:
(1) purple sweet potato after cleaning is crushed, the temperature for adding 1~2 times of its quality thereto after purple sweet potato is broken is
80~95 DEG C of water, and simultaneously according to 3~5 × 104Thermostable α-Amylase is added in the additive amount of U/kg, is 95 DEG C in temperature
Under the conditions of digest 1.5~2 hours；
(2) by after enzymatic hydrolysis purple sweet potato slurry be sent into juice extractor carry out first time squeezing, and will squeeze resulting potato slag according to
Water: slag=2:1 ratio squeeze for the second time after 45~50 DEG C of water resuspension extraction 30min is added, what merging was squeezed twice
It is filtered after purple sweet potato juice；
(3) purple sweet potato juice pH to 5.0, addition 4~4.5 × 10 are adjusted using citric acid5The carbohydrase of U/kg, in hydrolysis temperature
Saccharification enzymatic hydrolysis 2 hours under conditions of being 50-55 DEG C；
(4) pH is adjusted to 4.0 using citric acid by the purple sweet potato juice after saccharification, addition 1.5~2 × 105The pectin of U/kg
Enzyme digests 1 hour under conditions of hydrolysis temperature is 30；
(5) after digesting, bentonite is added according to 500~800mg/L, then adds 300~400mg/L activity
Charcoal is stood after stirring, filtering, collect filtrate, using hydrochloric acid deploy pH be 3-5 purple sweet potato juice.
2. preparing glutaraldehyde modified magnetic chitosan nano particle
(1) Fe is prepared3O4Magnetic core
A certain amount of FeCl is added into the water of nitrogen protection3·6H2O and FeCl2·4H2O(F3+With Fe2+Molar ratio is 2:
1), be substantially dissolved in it in water within mechanical stirring 5~10 minutes, to stablizing solution be added dropwise concentrated ammonia liquor to pH value be 10~
In 11,80 DEG C after water-bath 30min, product separation, distilled water in the case where adding magnetic field condition outside are sufficiently washed to neutrality, obtain Fe3O4Magnetic core
Solution for standby.
(2) chitosan magnetic nano particle is prepared
Using the Triton X-100 reverse phase water-in-oil microemulsion system of two-step method, 2% acetic acid of 1% chitosan concentration is prepared
Solution, and by magnetic core solution, acetic acid solution, hexamethylene and n-hexyl alcohol according to volume ratio be 4:4:11:6 ratio in 1800rpm
It is mixed under revolving speed, being then slowly added into Triton X-100 to lotion from black becomes white, then gradually becomes bright black
Until.It is eventually adding the 5mol/L NaOH of total volume about 4%, and in 60 DEG C after water-bath 120min, dehydrated alcohol and washing are each
It is spare that chitosan magnetic nanoparticles solution is obtained after 2~3 times.
(3) glutaraldehyde modified magnetic chitosan nano particle is prepared
By the chitosan magnetic nano particle constant volume of acquisition in 10mg/mL Fe3O4Concentration, in 0.5mol/L glutaraldehyde environment
After middle 150rpm revolving speed concussion activation 60min, it is standby that the modified chitosan magnetic nano particle of glutaraldehyde is obtained after washing 2-3 times
With.
3. adsorbing purple sweet potato anthocyanin
It takes and is placed in the purple sweet potato juice that pH is 3-5 in right amount by glutaraldehyde activated chitosan magnetic nano particle,
Completion in 1 hour is saturated adsorbing separation under the conditions of 150rpm concussion, after being saturated absorption, in the case where adding magnetic field condition outside to saturation
The chitosan magnetic nano particle of absorption purple sweet potato anthocyanin separate spare.
4. eluting purple sweet potato anthocyanin
The chitosan magnetic nano particle of saturation absorption purple sweet potato anthocyanin is placed in 65 DEG C of 90% methanol solution, methanol
Solution contains the hydrochloric acid of 0.1mol/L, handles 30min in the case where power is the ultrasound environments of 200W, chitosan magnetic can be completed and receive
Purple sweet potato anthocyanin elutes in rice grain, the purple sweet potato anthocyanin eluent after collecting externally-applied magnetic field separation chitosan magnetic nano particle
Spare, eluting rate reaches 80.5%.
5. preparing purple sweet potato anthocyanin freeze-dried powder
Purple sweet potato anthocyanin eluent is pumped into vacuum concentrating apparatus, is -0.6~-0.9Mpa, temperature 75 in vacuum degree
Vacuum concentration is completed at~85 DEG C, until in concentrate without methanol or ethyl alcohol, collects purple sweet potato anthocyanin concentrate and evaporation
Liquid.Then by the purple sweet potato anthocyanin concentrate of collection in the environment of -18 DEG C pre-freeze 5~6 hours, temperature be -50 DEG C, vacuum
Degree is freeze-dried 24~30 hours under conditions of being 25~50Pa, obtains the purple sweet potato anthocyanin freeze-dried powder that purity is 96.8%.
Embodiment 2
This embodiment differs from embodiment 1 in that the elution of purple sweet potato anthocyanin and the system of purple sweet potato anthocyanin freeze-dried powder
It is standby, specific as follows:
4. eluting purple sweet potato anthocyanin
The chitosan magnetic nano particle of saturation absorption purple sweet potato anthocyanin is placed in 55 DEG C of 70% methanol solution, methanol
Containing the hydrochloric acid of 0.1mol/L in solution, 45min is handled in the case where power is the ultrasound environments of 200W, chitosan magnetic can be completed
Purple sweet potato anthocyanin elutes on nano particle, the purple sweet potato anthocyanin elution after collecting externally-applied magnetic field separation chitosan magnetic nano particle
Liquid is spare, and eluting rate reaches 67.5%.
5. preparing purple sweet potato anthocyanin freeze-dried powder
Purple sweet potato anthocyanin eluent is pumped into vacuum concentrating apparatus, is -0.6~-0.9Mpa, temperature 75 in vacuum degree
Vacuum concentration is completed at~85 DEG C, until in concentrate without methanol or ethyl alcohol, collects purple sweet potato anthocyanin concentrate and evaporation
Liquid.By the purple sweet potato anthocyanin concentrate of collection in the environment of -18 DEG C pre-freeze 5~6 hours, temperature is -50 DEG C, vacuum degree is
It is freeze-dried 24~30 hours under conditions of 25~50Pa, obtains the purple sweet potato anthocyanin freeze-dried powder that purity is 95.7%.
Claims (10)
1. a kind of method with glutaraldehyde modified magnetic chitosan nano particle separation purple sweet potato anthocyanin, which is characterized in that including
Following steps:
S1: purple sweet potato juice and the glutaraldehyde modified magnetic chitosan nano particle that pH is 3~5 are prepared respectively；
S2: glutaraldehyde modified magnetic chitosan nano particle is placed in the purple sweet potato juice that pH is 3~5, is carried out under the conditions of earthquake
Saturation absorption；
S3: in the glutaraldehyde modified magnetic chitosan nano for isolating saturation absorption purple sweet potato anthocyanin outside plus under magnetic field condition
Grain；
S4: the glutaraldehyde modified magnetic chitosan nano particle for the saturation absorption purple sweet potato anthocyanin isolated is placed in containing hydrochloric acid
55~65 DEG C of organic solvent in be ultrasonically treated, obtain purple sweet potato anthocyanin eluent；
S5: purple sweet potato anthocyanin eluent is concentrated in vacuo, is freeze-dried to obtain purple sweet potato anthocyanin freeze-dried powder.
2. a kind of side with glutaraldehyde modified magnetic chitosan nano particle separation purple sweet potato anthocyanin according to claim 1
Method, which is characterized in that in the step S1 preparing pH be 3~5 purple sweet potato juice comprising steps of
A1 it) will be crushed after the cleaning of light violet potato, water and Thermostable α-Amylase be added in broken purple sweet potato, enzymatic hydrolysis 1.5~2 is small
When after purple sweet potato slurry；
A2) squeezing purple sweet potato starches to obtain purple sweet potato juice；
A3 carbohydrase is added after) adjusting the pH of purple sweet potato juice, carries out saccharification enzymatic hydrolysis under conditions of hydrolysis temperature is 50-55 DEG C；
A4 it) adjusts after saccharification digests and pectase is added after the pH of purple sweet potato juice, digested under conditions of hydrolysis temperature is 30；
A5) sequentially add bentonite, active carbon is stood after stirring, filtering, using hydrochloric acid deploy pH be 3~5 purple sweet potato juice.
3. a kind of side with glutaraldehyde modified magnetic chitosan nano particle separation purple sweet potato anthocyanin according to claim 1
Method, which is characterized in that in the step S1 prepare glutaraldehyde modified magnetic chitosan nano particle comprising steps of
B1) by FeCl3·6H2O and FeCl2·4H2O is added in the water of nitrogen protection, and stirring adjusts pH to 10~11,80 DEG C of water
After bath, the separation product in the case where adding magnetic field condition outside, and Fe is obtained to neutrality with the abundant washed product of distilled water3O4Magnetic core solution；
B2 the acetic acid solution for) preparing chitosan-containing, by Fe3O4After magnetic core solution, acetic acid solution, hexamethylene and n-hexyl alcohol mixing
Lotion, Triton X-100 is slowly added into gained lotion to lotion from black becomes white, continuously adds Triton X-
100 gradually become black to lotion, and NaOH, water-bath is added, and dehydrated alcohol washs respectively with water, obtain chitosan magnetic nano particle
Solution；
B3) by the chitosan magnetic nanoparticles solution constant volume of acquisition in Fe3O4Concentration shakes activation in glutaraldehyde, washes,
Obtain glutaraldehyde modified magnetic chitosan nano particle.
4. a kind of side with glutaraldehyde modified magnetic chitosan nano particle separation purple sweet potato anthocyanin according to claim 3
Method, which is characterized in that the step b1) it specifically includes: FeCl is added into the water of nitrogen protection3·6H2O and FeCl2·
4H2O, and make F3+With Fe2+Molar ratio is 2:1, stirs 5~10 minutes, makes FeCl3·6H2O and FeCl2·4H2O is completely dissolved in
In water, be added dropwise into the aqueous solution after stabilization concentrated ammonia liquor to pH be 10~11, water-bath 30min in 80 DEG C later, by gained
Product is separated outside plus under magnetic field condition, and is washed with distilled water to neutrality, obtains Fe3O4Magnetic core solution.
5. a kind of side with glutaraldehyde modified magnetic chitosan nano particle separation purple sweet potato anthocyanin according to claim 3
Method, which is characterized in that the Fe3O4Magnetic core solution, acetic acid solution, hexamethylene and n-hexyl alcohol volume ratio be 4:4:11:6.
6. a kind of side with glutaraldehyde modified magnetic chitosan nano particle separation purple sweet potato anthocyanin according to claim 3
Method, which is characterized in that the step b3) it specifically includes: by the chitosan magnetic nano particle constant volume of acquisition in 10mg/mL
Fe3O4Concentration washes 2-3 times in 0.5mol/L glutaraldehyde environment after 150rpm revolving speed concussion activation 60min, obtains glutaraldehyde and change
Property chitosan magnetic nano particle.
7. a kind of side with glutaraldehyde modified magnetic chitosan nano particle separation purple sweet potato anthocyanin according to claim 1
Method, which is characterized in that the step S2 is specifically included: it is 3~5 that glutaraldehyde modified magnetic chitosan nano particle, which is placed in pH,
In purple sweet potato juice, 150rpm shakes the saturation absorption of completion in 1 hour.
8. a kind of side with glutaraldehyde modified magnetic chitosan nano particle separation purple sweet potato anthocyanin according to claim 1
Method, which is characterized in that the step S4 is specifically included: by the glutaraldehyde modified magnetic for the saturation absorption purple sweet potato anthocyanin isolated
Chitosan nano particle is placed in 55~65 DEG C of the methanol solution or ethanol solution containing hydrochloric acid, and wherein concentration of hydrochloric acid is
0.1mol/L handles 30~45min in the case where power is the ultrasound environments of 150~250W, obtains purple sweet potato anthocyanin eluent.
9. a kind of side with glutaraldehyde modified magnetic chitosan nano particle separation purple sweet potato anthocyanin according to claim 7
Method, which is characterized in that the methanol solution or ethanol solution are the methanol solution that volume ratio is 85~95% or volume ratio is 65
~75% ethanol solution.
10. a kind of glutaraldehyde modified magnetic chitosan nano particle separation purple sweet potato anthocyanin according to claim 1
Method, which is characterized in that the step S5 is specifically included: purple sweet potato anthocyanin eluent being pumped into vacuum concentrating apparatus, true
Reciprocal of duty cycle is -0.6~-0.9Mpa, temperature is concentrated in vacuo under conditions of being 75~85 DEG C, until without methanol or second in concentrate
Until alcohol；Purple sweet potato anthocyanin concentrate and evaporation liquid are collected, the purple sweet potato anthocyanin concentrate of collection is pre- in the environment of -18 DEG C
Freeze 5~6 hours, is freeze-dried 24~30 hours, obtains pure under conditions of temperature is -50 DEG C, vacuum degree is 25~50Pa later
The purple sweet potato anthocyanin freeze-dried powder that degree is 95~98%.
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| CN109999767A (en) * | 2019-04-17 | 2019-07-12 | 江苏师范大学 | A kind of method of glutaraldehyde modification of chitosan resin adsorption separation purple sweet potato anthocyanin |
| WO2020224327A1 (en) * | 2019-12-07 | 2020-11-12 | 江苏省农业科学院 | Method of using magnetic nano-vector material to purify plant anthocyanin |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109999767A (en) * | 2019-04-17 | 2019-07-12 | 江苏师范大学 | A kind of method of glutaraldehyde modification of chitosan resin adsorption separation purple sweet potato anthocyanin |
| WO2020224327A1 (en) * | 2019-12-07 | 2020-11-12 | 江苏省农业科学院 | Method of using magnetic nano-vector material to purify plant anthocyanin |
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| CN109134414B (en) | 2020-07-28 |
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