CN1896225A - Liquid-cultured flesh lucid ganoderma, and production of flesh lucid ganoderma nutrient liquid by culturing liquid - Google Patents
Liquid-cultured flesh lucid ganoderma, and production of flesh lucid ganoderma nutrient liquid by culturing liquid Download PDFInfo
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- CN1896225A CN1896225A CN 200610017640 CN200610017640A CN1896225A CN 1896225 A CN1896225 A CN 1896225A CN 200610017640 CN200610017640 CN 200610017640 CN 200610017640 A CN200610017640 A CN 200610017640A CN 1896225 A CN1896225 A CN 1896225A
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Abstract
Method for culturing glossy ganoderma by liquid and preparing glossy ganoderma alimentative liquid by culturing liquid is carried out by inoculating glossy ganoderma rope in culturing liquid to activate under aseptic condition, laying aside to culture for 68-108days, acquiring doubled and redoubled glossy ganoderma rope, removing impurities by alkali washing liquor, absorbing 90% supernatant of crude liquid and filling to obtain the final product. It is nutritious and makes the full use of glossy ganoderma.
Description
Technical field:
The present invention relates to a kind of liquid-cultured flesh lucid ganoderma and utilize the method for its inoculum preparation Ganoderma nutritive medium, belong to microbial cells and cultivate and nutritional health food field.
Background technology:
Ganoderma is a kind of fungus body under the virgin state in period in ancient times, is called as all vegeto-animal ancestors.Claim Ganoderma to be in [The Classic of Mountains and Rivers]: to look meat, envelope.Modern age, famous scholar Guo Pu called: " look meat shape such as beef liver, two orders are arranged, the endless of food sought demutation as usual also." Ming Dynasty physician LI Shi-Zhen puts down in writing in [Compendium of Materia Medica]: " and meat sesame shape such as meat invest Da Shi, all have end to end, and white person is as cutting a fat, yellow person such as Zijin, and food can be made light of one's life by commiting suicide not oldly for a long time, and the angle prolongs life." and claim that Ganoderma is that book on Chinese herbal medicine is top grade; but because Ganoderma is of uncertain whereabouts; very rare; thereby at the veil of being coverd with all mysteries among the people; people address respectfully Ganoderma and are " local tyrant " in history; look for wonder or keep someone at a respectful distance, or probably keep away it, cause originally with regard to the endangered circumstances of rare rare species.
The beginning of the eighties in last century, the Xian City, Shanxi Province has been found the trace of Ganoderma first, after the public exhibition of capital, caused showing great attention to of the Chinese government and foreign dignitary, the Chinese Academy of Sciences, the expert of scientific research departments such as Northwest University begins to utilize modern scientific method to the contained nucleic acid of Ganoderma, the glossy ganoderma polypeptide, polysaccharide, geotrichum candidum, pyrroles's beautiful jade ketone quinone, tens of kinds of rare nutritive substances such as selenium have carried out the detection and the analysis of science, now are identified as " super-huge rare sticking complex body ".The attention of government has caused that also domestic and international scientific circles have launched excavation to the using value of Ganoderma, although present different types of mushroom pharmacy and nutritious prod constantly appear on the market, but because the Ganoderma thalline is very in great shortage, artificial culture still belongs to blank, so really take the product development utilization as the leading factor, be still a difficult problem that needs to be resolved hurrily at present with Ganoderma.
Summary of the invention:
The purpose of this invention is to provide a kind of liquid-cultured flesh lucid ganoderma and utilize the method for its inoculum preparation Ganoderma nutritive medium, can gather in the crops the Ganoderma bacterial spawn of body at double in the short period of time with this method, for Ganoderma in scientific research, medicinal, the application of aspects such as nutritive health-care provides precious resources.Another object of the present invention provides Ganoderma nutrition oral administration stoste, fundamentally reduce the production cost of Ganoderma nutrition oral administration, Ganoderma thalline of the present invention is contriver's protista Ganoderma viable bacteria body that the county found in Qinghai Province is bright in 1993, and nutrient solution is that collection contriver cultivation in 20 years Ganoderma experience accumulates and perfect secret recipe is formed.
Solution of the present invention is as follows:
Under the aseptic condition Ganoderma thalline is inoculated in the nutrient solution and activates, open cultivation is 68-108 days under the state that leaves standstill, and gathers in the crops the Ganoderma viable bacteria body of body at double; Get the nutrient solution 200-500kg that obtains behind the Ganoderma viable bacteria body and be heated to 58 degree, last ion tower is removed impurity with the alkaline rinse wash-out, collects elutriant and precipitates, and filters to obtain 80% supernatant liquor through can, checks, and label is packed.Be Ganoderma nutrition oral administration stoste
1. it is formulated that the Ganoderma nutrient solution has following substances in percentage by weight: genseng 5-8%, Prinsepia utilis 10-15%, donkey-hide gelatin 5-15%, royal jelly 10-20%, red date 10-15%, peptone 5-10%, rhizoma cyperi 1-3%, Medicated Leaven 2-6%, Chinese yam 10-20%, Styrax 2-6%.
2. Ganoderma thalline live body is cultivated:
A. get genseng, Prinsepia utilis, red date, rhizoma cyperi, Chinese yam, Styrax is cleaned and to be drained away the water, and puts into pottery or stainless steel vessel and adds water and be warming up to 100 degree sterilizations and pull out after 30 minutes, and the earthenware porcelain receptacle of putting into the phreatic water that fills 200kg again soaked 48 hours.
B is warming up to 58 degree with above-mentioned soak solution, adds donkey-hide gelatin, and royal jelly after peptone, Medicated Leaven fully stir, was got supernatant liquor spontaneous fermentation 72 hours, was the Ganoderma bacterial culture fluid.
C puts into nutrient solution with the Ganoderma thalline, open cultivation is 68-108 days under the condition of 22--28 degree, the Ganoderma thalline activates during this period naturally, quick times of body increases, and the 1kg thalline can double to 2--5kg finished product Ganoderma, finished product Ganoderma shape such as meat shape, layering, the surface is the oyster white colloid, and middle level and lower floor are that dark meat is fibrous, have stronger tart flavour.
The d aseptic condition takes out finished product Ganoderma viable bacteria body down, nutrient solution supernatant liquor sucking-off with 90% is as Ganoderma nutrition oral administration stoste, remainder adds again uses a, puts into the Ganoderma thalline after the nutrient solution that b two methods obtain mixes again and continues to cultivate, and forms the round-robin training mode.
3. the preparation method of Ganoderma nutrition oral administration:
Take after Ganoderma nutrition oral administration stoste that the d method obtains grinds with colloidal mill.Be heated to 58 degree, last ion tower is removed impurity with the alkaline rinse wash-out, collects elutriant and precipitates 72 hours, obtains 80% supernatant liquor after the filtration, through can, and check, label, packing.Be the Ganoderma nutrition oral administration.
4. the dregs of a decoction and the W-Gum of gained in above-mentioned throw out and the Ganoderma nutrient solution are pressed 0.Example mixed in 2: 1: 1, added an amount of moistening granulation of 70% ethanol, in the hard capsule of packing into after the drying, was the Ganoderma nourishing capsule.
It is activeconstituents that nutrition oral administration of the present invention contains the extract for the treatment of significant quantity, and contains one or more pharmaceutically acceptable carriers.
Above-mentioned carrier is meant the medicine carrying agent of pharmaceutical field routine, comprises thinner, vehicle, weighting agent, wetting agent, absorption enhancer, absorption carrier.
Nutrition oral administration preferred weight ratio of the present invention is the 1-99% activeconstituents, preferably contains 10-90%, more preferably 20-80%, best preferred 68% nutrient solution of the present invention.
Every day of the present invention, consumption was: Ganoderma nutrition oral administration 50-100 milliliter, Ganoderma nourishing capsule 12-18g divides and takes for 2-3 time.
The present invention has following positively effect: utilize liquid-based to cultivate the Ganoderma thalline, can not only gather in the crops the Ganoderma viable bacteria body of body at double, realized the fast breeding of Ganoderma, make it the industrialization development condition and be tending towards ripe, can also from its nutrient solution, prepare Ganoderma nutrition oral administration and Ganoderma nourishing capsule, have adjusting and improve immune function of human body, antiviral, anti-infective, the gentle fatigue, physical strength reinforcing and hypotensive, blood fat of separating of anti-canceration, blood sugar, effect such as delay senility.Be fit to suitability for industrialized production and family and cultivate, the scientific research and the nutritional application that have improved Ganoderma are worth.
Embodiment:
Embodiment 1
Get genseng 5-8%, Prinsepia utilis 10-15%, red date 10-15%, rhizoma cyperi 1-3%, Chinese yam 10-20%, Styrax 2-6%, clean and drain away the water, putting into pottery or stainless steel vessel adds the water sterilization that heats up and pulls out after 30 minutes to put into and fill 200kg phreatic water and soak and be warming up to 38 degree in 48 hours again, add donkey-hide gelatin 5-15%, royal jelly 10-20%, peptone 5-10%, Medicated Leaven 2-6% fully stirs, draw supernatant liquor 190kg spontaneous fermentation 72 hours then, aseptic condition is transferred incarnation glossy ganoderma thalline, leave standstill open cultivation 68-108 days at room temperature 22 degree-28 degree, gather in the crops body Ganoderma viable bacteria body at double.
Embodiment 2
Get the nutrient solution 180kg that embodiment 1 cultivates behind the Ganoderma thalline and grind with colloidal mill, last ion tower is removed impurity with alkaline rinse (ammoniacal liquor of 0.5N) wash-out, and the collection elutriant precipitates 72 hours, gets the supernatant liquor can and gets the Ganoderma nutrition oral administration.
Embodiment 3
Get the dregs of a decoction of embodiment 2 throw outs and embodiment 1 gained and W-Gum and evenly add the moistening granulation of an amount of 70% ethanol, make the Ganoderma nourishing capsule in the hard capsule of packing into after the drying by 0.2: 1: 1 mixed.
Practical example 1
Ganoderma bacterial culture fluid: genseng 200g, Prinsepia utilis 500g, red date 800g, rhizoma cyperi 100g, Chinese yam 1000g, Styrax 100g cleans and to drain away the water, and puts into pottery or stainless steel vessel and adds the water sterilization that heats up and pull out after 30 minutes to put into and fill 200kg phreatic water and soak and be warming up to 38 degree in 48 hours again, add donkey-hide gelatin 500g, royal jelly 800g, peptone 600g, Medicated Leaven 200g fully stirs, draw supernatant liquor 190kg spontaneous fermentation 72 hours then, aseptic condition is transferred incarnation glossy ganoderma thalline, leaves standstill open cultivation 68-108 days at room temperature 22 degree-28 degree, gathers in the crops the Ganoderma viable bacteria body of body at double.
Practical example 2
Ganoderma nutrition oral administration: get Ganoderma nutrient solution (practical example 1 gained) 190g, grind with colloidal mill, last ion tower is removed impurity with alkaline rinse (ammoniacal liquor of 0.5N) wash-out, collected the elutriant precipitation 72 hours, draw supernatant liquor 180kg can, every bottle of nt wt net weight is 20 milliliters or 100-500 milliliter, promptly gets the Ganoderma nutrition oral administration, and sundry item should meet the requirement of 2000 editions relevant nutrition oral administrations of Pharmacopoeia of People's Republic of China.
Practical example 3
Ganoderma capsule: get the dregs of a decoction of Ganoderma nutrition oral administration throw out (practical example 2 gained) and practical example 1 gained and the evenly moistening granulation of an amount of 70% ethanol of adding of mixed that W-Gum is pressed 0.2: 1: 1, divide in the hard capsule of packing into after dry and make the Ganoderma nourishing capsule, the heavy 0.3g of each capsule.Sundry item should meet 2000 editions relevant capsular requirements of Pharmacopoeia of People's Republic of China.
Specific examples of the present invention can change according to the plus-minus of composition and many similar prescriptions and technical process, as long as same or similar with prescription formulation in this document, no matter adopts which kind of technical process processing, all belongs to the protection domain of patent of the present invention.
Claims (5)
1. a liquid-cultured flesh lucid ganoderma and utilize the method for its inoculum preparation Ganoderma nutritive medium, be to utilize modern biotechnology and conventional trophology principle that the Ganoderma bacterial spawn is seeded in the bio-culture solution under the aseptic condition, it is characterized in that: leave standstill cultivation, naturally activate, times body increases, results 2-5kg finished product Ganoderma.
2. a liquid-cultured flesh lucid ganoderma and utilize the method for its inoculum preparation Ganoderma nutritive medium, it is characterized in that: concentrate and to collect the nutrient solution supernatant liquor 200-500kg that cultivates behind the Ganoderma thalline, be heated to the last ion tower of 58 degree and remove impurity with the alkaline rinse wash-out, the collection supernatant liquor refilters the 80-90% can to original volume, obtains the Ganoderma nutrition oral administration.
3. the Ganoderma nutrition oral administration according to claim 2 gained meets pharmaceutically acceptable carrier.
4. according to claim 2, the Ganoderma nutrition oral administration of 3 gained, preferred weight ratio are the activeconstituents of 1-99% nutrient solution, preferably contain 10-90%, more preferably 20-80%, and the best is preferably 68% nutrient solution of the present invention.
5. a liquid-cultured flesh lucid ganoderma and utilize the method for its inoculum preparation Ganoderma nutritive medium, it is characterized in that: the Ganoderma nutrient solution is made of following substances in percentage by weight: genseng 5-8%, Prinsepia utilis 10-15%, donkey-hide gelatin 5-15%, royal jelly 10-20%, red date 10-15%, peptone 5-10%, rhizoma cyperi 1-3%, Medicated Leaven 2-6%, Chinese yam 10-20%, Styrax 2-6%, phreatic water 200kg.
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| CN 200610017640 CN1896225A (en) | 2006-04-13 | 2006-04-13 | Liquid-cultured flesh lucid ganoderma, and production of flesh lucid ganoderma nutrient liquid by culturing liquid |
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| CN 200610017640 CN1896225A (en) | 2006-04-13 | 2006-04-13 | Liquid-cultured flesh lucid ganoderma, and production of flesh lucid ganoderma nutrient liquid by culturing liquid |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101117620B (en) * | 2007-07-18 | 2010-07-07 | 王朝江 | Method for acquiring tangible Taisui bacterial strain |
| CN101624570B (en) * | 2008-07-07 | 2011-04-13 | 刘志军 | Method for cultivating cultured ganoderma lucidum with Chinese medicaments and method for preparing cultured ganoderma lucidum health-care product |
| CN102113448A (en) * | 2010-12-08 | 2011-07-06 | 马兰英 | Rapid cultivation method for high-quality meat lucid ganoderma |
| CN102987489A (en) * | 2012-12-14 | 2013-03-27 | 邓江莲 | Chinese yam juice drink and preparation method thereof |
| CN105685965A (en) * | 2016-01-18 | 2016-06-22 | 杨发国 | Production technology of Ganoderma lucidum blood-purifying toxicity-removing stomach-invigorating cancer-resisting body-building health care liquid |
| CN106472108A (en) * | 2016-10-09 | 2017-03-08 | 湖北九公堂生物科技开发有限责任公司 | A kind of new method of the raw material cultivation meat glossy ganoderma with integration of drinking and medicinal herbs |
| CN108713447A (en) * | 2018-06-01 | 2018-10-30 | 韩秀花 | A kind of high altitude localities local tyrant meat Mythic Fungus cultivation method and its health products preparation method |
| CN111304118A (en) * | 2020-02-19 | 2020-06-19 | 白山太岁生物科技有限公司 | Culture solution for myxomycete and method for culturing myxomycete |
| CN113116943A (en) * | 2021-04-27 | 2021-07-16 | 江苏春江生物科技有限公司 | Preparation method and device for treating blood sugar by fermenting Chinese herbal medicines for the aged |
-
2006
- 2006-04-13 CN CN 200610017640 patent/CN1896225A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101117620B (en) * | 2007-07-18 | 2010-07-07 | 王朝江 | Method for acquiring tangible Taisui bacterial strain |
| CN101624570B (en) * | 2008-07-07 | 2011-04-13 | 刘志军 | Method for cultivating cultured ganoderma lucidum with Chinese medicaments and method for preparing cultured ganoderma lucidum health-care product |
| CN102113448A (en) * | 2010-12-08 | 2011-07-06 | 马兰英 | Rapid cultivation method for high-quality meat lucid ganoderma |
| CN102987489A (en) * | 2012-12-14 | 2013-03-27 | 邓江莲 | Chinese yam juice drink and preparation method thereof |
| CN105685965A (en) * | 2016-01-18 | 2016-06-22 | 杨发国 | Production technology of Ganoderma lucidum blood-purifying toxicity-removing stomach-invigorating cancer-resisting body-building health care liquid |
| CN106472108A (en) * | 2016-10-09 | 2017-03-08 | 湖北九公堂生物科技开发有限责任公司 | A kind of new method of the raw material cultivation meat glossy ganoderma with integration of drinking and medicinal herbs |
| CN108713447A (en) * | 2018-06-01 | 2018-10-30 | 韩秀花 | A kind of high altitude localities local tyrant meat Mythic Fungus cultivation method and its health products preparation method |
| CN111304118A (en) * | 2020-02-19 | 2020-06-19 | 白山太岁生物科技有限公司 | Culture solution for myxomycete and method for culturing myxomycete |
| CN113116943A (en) * | 2021-04-27 | 2021-07-16 | 江苏春江生物科技有限公司 | Preparation method and device for treating blood sugar by fermenting Chinese herbal medicines for the aged |
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Open date: 20070117 |