CN1888046A - Process of producing live microbial composition EM prepn - Google Patents
Process of producing live microbial composition EM prepn Download PDFInfo
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- CN1888046A CN1888046A CN 200610019753 CN200610019753A CN1888046A CN 1888046 A CN1888046 A CN 1888046A CN 200610019753 CN200610019753 CN 200610019753 CN 200610019753 A CN200610019753 A CN 200610019753A CN 1888046 A CN1888046 A CN 1888046A
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Abstract
The live microbial composition EM preparation is produced with rice washing water and through regulating pH value, treating with neutral proteinase, adding certain amount of one of waste molasses, red sugar and starch saccarifying slurry, phosphate, table salt, sulfate and yeast paste, mixing homogeneously, heating at 100 deg.c for 5 min, cooling, inoculating live EM bacterial liquid in 10-20 %, and fermentation to culture. The present invention has simple production process suitable for industrial production and low cost, and can produce high quality live microbial composition EM preparation with high live bacteria content for use in farm production, livestock breeding, food processing, environment protection and other fields.
Description
Technical field
The present invention relates to a kind of production method of live microbial composition EM preparation.
Background technology
EM is the english abbreviation of effective microorganism (efective microorganisms), is present more common a kind of probiotics.Mainly belonging to 80 kinds of microorganisms by photosynthetic bacterium, milk-acid bacteria, actinomycetes, fermented type thread fungus etc. 10 and form, is the special flora that a kind of anaerobism and oxytropism have both at the same time.It at first is that Japanese Ryukyu university studies successful a kind of NEW TYPE OF COMPOSITE microorganism live bacteria preparation than good according to people such as professors husband.EM does not contain any chemical objectionable impurities, has no side effect, and noresidue and secondary pollution do not produce antiradiation drug.Since coming out the eighties in 20th century, promote the use of in more than 60 countries and regions such as Japan, Brazil, the U.S., Thailand, all show good effect at aspects such as promoting plant-growth, enhancing livestock and poultry resistance against diseases, the immunizing power that strengthens the breed object, removal ight soil stench, improve the ecological environment and improve the quality of products.EM introduces China the beginning of the nineties, promote through these years experimental study, has obtained significant effect in fields such as non-polluted planting industry, aquaculture and environment protection, and the Sustainable development of agricultural has been produced huge pushing effect.
After EM introduced China, through relevant scientific and technical personnel's continuous exploration, research and development, its culture technique was grasped substantially, and culture medium prescription presents variation.A kind of preparation method of nutrient solution of the EM of being applicable to viable bacteria production is disclosed as Chinese patent application " preparation method of the nutrient solution that a kind of EM of being applicable to viable bacteria produces " (application number 02145041.2), this method is that raw material is made juice with the potato, adding relatively large sucrose, glucose, honey, sodium bicarbonate then mixes and stirs evenly, make after the nutrient solution, add the EM viable bacteria by 1: 1 volume ratio, be greater than or less than the cultivation of room temperature bottom fermentation.This culture medium prescription, the raw material that is adopted is more expensive, the cost height, nutrition is owed comprehensively comparatively speaking, does not add as micro-sulphur, phosphorus, potassium, magnesium etc., will certainly have influence on the growth of EM viable bacteria like this, thereby have influence on the quality of product.
Summary of the invention
The technical problem to be solved in the present invention provides that a kind of manufacturing process is simple, culture medium cost is low, the production method of the comprehensive EM live bacteria agent of nutritive substance.
The present invention solves the problems of the technologies described above with following technical scheme:
After the rice washing water pH value transferred to neutrality, (as follows) was that the ratio of 0.001%-0.01% adds neutral protease by weight percentage, is heated to 40～50 ℃, be incubated 1 hour, every stirring in 10 minutes once; To add waste molasses or brown sugar (Saccharum Sinensis Roxb.) or plant amylum saccharification slurries, phosphoric acid salt, magnesium salts, salt, yeast extract paste, mixing and stirring by a certain percentage through pretreated rice washing water, be heated to 100 ℃, 5 minutes; To be cooled to below 40 ℃, add EM strain liquid mixing secondary fermentation in 10～20% ratio then and promptly get live bacteria agent.
Contain abundant carbohydrate, protein, vitamin B group, phosphorus, potassium and various trace element in the rice washing water, be of high nutritive value, after the EM viable bacteria fermentation transforms, nutritive value and result of use further improve, and all show better effect at aspects such as promoting plant-growth, enhancing livestock and poultry resistance against diseases, the immunizing power that strengthens the breed object, removal ight soil stench, improve the ecological environment and improve the quality of products.And use method of the present invention to produce the EM live bacteria agent, simple and reliable process, with low cost, suitable suitability for industrialized production.
Embodiment
With the major ingredient of rice washing water (also claiming washing water of rice) as bacteria culture fluid, utilize in the rice washing water unique nutrition and microorganism species efficiently, add feed supplements such as small amounts of inorganic salt, waste molasses, can produce high-quality EM living bacterial liquid, not only product performance are superior, can also reduce the plant effuent quantity discharged, reduce environmental pollution, reach the purpose that turns waste into wealth.
Method technological process of the present invention is as follows:
1, the pre-treatment of rice washing water:
Rice washing water can be used (Na)
2CO
3Solution transfers to about PH7.0, adds neutral protease in the 0.001%-0.01% ratio then, is heated to 40～50 ℃, is incubated 1 hour, stirs once every 10 minutes.
2, culture medium preparation and fermentation:
The pretreated rice washing water of learning from else's experience adds waste molasses or plant amylum saccharification slurries or brown sugar (Saccharum Sinensis Roxb.), phosphoric acid salt, magnesium salts, salt, yeast extract paste, mixing and stirring by a certain percentage, is heated to 100 ℃, 5 minutes.To be cooled to below 40 ℃, the ratio in 10～20% adds the EM strain liquid and mixes, and places plastic seal container or acidproof fermentor tank, and fermentation promptly gets the live bacteria agent product.Above-mentioned culture medium prescription can be: add useless sugared close 1-3% or mashing slurries 8-10% or brown sugar (Saccharum Sinensis Roxb.) 1-2% in the rice washing water, NaCl 0.1-0.3%, KH
2PO
40.1-0.3%, MgSO
40.01-0.03%, yeast extract 0.1-0.3%.
Above-mentioned leavening temperature is between 20～37 ℃, and the length of fermentation time is decided according to the temperature of fermentation.
Can also adopt with the rice washing water is that main raw material is made various types of rice washing water nutrient solutions.
Embodiment 1
Get rice: the slubbing rice washing water 1000g of wash water=1: 1 (weight ratio), use Na
2CO
3After solution transfers to PH7.0, add neutral protease 0.1g (0.01%), stir, be heated to 40 ℃ of insulations and handled 1 hour, middle every stirring in 10 minutes 1 time.Add NaCl 1g (0.1%) then, waste molasses 20g (2%), KH
2PO
41g (0.1%), MgSO
40.2g (0.02%), yeast extract 1g (0.1%), mixing and stirring was boiled 5 minutes, and is to be cooled to 35 ℃, inoculation EM viable bacteria 150g (15%), mixing is put in the plastics pot and is sealed, and 24～28 ℃ of leavening temperatures, fermentation time are 96 hours.PH is 3.5, and bacterial count reaches 3.6 * 10
9Individual/ml, smell acid is with fragrant and sweet.
Embodiment 2
Get rice: the slubbing rice washing water 500kg of wash water=1: 2 (weight ratio) adds an amount of Na
2CO
3After solution transfers to PH7.0, add neutral protease 50g (0.01%), stir, heat 45 ℃ of insulation enzymes and handled 1 hour, middle every stirring in 10 minutes 1 time.Add NaCl 1kg (0.2%) then, waste molasses 10kg (2%), KH
2PO
41kg (0.2%), MgSO
40.075kg (0.015%), yeast extract 1.0kg (0.2%), mixing and stirring, heat 100 ℃ 5 minutes, to be cooled to 35～40 ℃, the inoculation EM viable bacteria 50kg (10%), mix, leavening temperature is 33 ℃, fermentation time is 85 hours.PH is 3.5, and bacterial count reaches 3.7 * 10
9Individual/ml, smell acid is with fragrant and sweet.
Embodiment 3
Get rice: the slubbing rice washing water 500kg of wash water=1: 2.5 (weight ratio) adds an amount of Na
2CO
3After solution transfers to PH7.0, add neutral protease 20g (0.004%), stir, heat 45 ℃ of insulation enzymes and handled 1 hour, middle every stirring in 10 minutes 1 time.Add NaCl 1.5kg (0.3%) then, mashing slurries 45kg (9%), KH
2PO
41.5kg MgSO (0.3%),
40.075kg (0.015%), yeast extract 1kg (0.2%), mixing and stirring, heat 100 ℃ 5 minutes, to be cooled to 35～40 ℃, the inoculation EM viable bacteria 50kg (10%), mix, leavening temperature is 35 ℃, fermentation time is 85 hours.PH is 3.5, and bacterial count reaches 3.7 * 10
9Individual/ml, smell acid is with fragrant and sweet.
Embodiment 4
Get rice: the slubbing rice washing water 500kg of wash water=1: 2.8 (weight ratio) adds an amount of Na
2CO
3After solution transfers to PH7.0, add neutral protease 5g (0.001%), stir, heat 45 ℃ of insulation enzymes and handled 1 hour, middle every stirring in 10 minutes 1 time.Add NaCl 0.5kg (0.1%) then, brown sugar (Saccharum Sinensis Roxb.) 5kg (1%), KH
2PO
40.5kg MgSO (0.1%),
40.15kg (0.03%), yeast extract 1.5kg (0.3%), mixing and stirring, heat 100 ℃ 5 minutes, to be cooled to 35～40 ℃, the inoculation EM viable bacteria 100kg (20%), mix, leavening temperature is 36 ℃, fermentation time is 72 hours.PH is 3.4, and bacterial count reaches 4.6 * 10
9Individual/ml, smell acid is with fragrant and sweet.
Claims (1)
1. the production method of a live microbial composition EM preparation, after it is characterized in that the rice washing water pH value transferred to neutrality, be that the ratio of 0.001%-0.01% adds neutral protease by weight percentage, be heated to 40～50 ℃, be incubated 1 hour, stirred once every 10 minutes; To add waste molasses or brown sugar (Saccharum Sinensis Roxb.) or plant amylum saccharification slurries, phosphoric acid salt, magnesium salts, salt, yeast extract paste, mixing and stirring by a certain percentage through pretreated rice washing water, be heated to 100 ℃, 5 minutes; To be cooled to below 40 ℃, the ratio in 10～20% adds EM strain liquid mixing secondary fermentation and promptly gets live bacteria agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100197532A CN100424165C (en) | 2006-07-27 | 2006-07-27 | Process of producing live microbial composition EM prepn |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100197532A CN100424165C (en) | 2006-07-27 | 2006-07-27 | Process of producing live microbial composition EM prepn |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1888046A true CN1888046A (en) | 2007-01-03 |
| CN100424165C CN100424165C (en) | 2008-10-08 |
Family
ID=37577331
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|---|---|---|---|
| CNB2006100197532A Active CN100424165C (en) | 2006-07-27 | 2006-07-27 | Process of producing live microbial composition EM prepn |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101591631B (en) * | 2009-06-08 | 2011-04-13 | 江西省天意生物技术开发有限公司 | EM original dew and production method thereof |
| CN102911903A (en) * | 2012-10-29 | 2013-02-06 | 丘佩珍 | Cultivation of lactic acid bacteria and method of keeping nuisance-free rabbits with lactic acid bacteria |
| CN103255074A (en) * | 2012-02-15 | 2013-08-21 | 何寒 | Simple and rapid propagation method of EM |
| CN103255059A (en) * | 2012-02-15 | 2013-08-21 | 何寒 | Preparation method of high purity EM protospecies |
| CN104531802A (en) * | 2014-12-11 | 2015-04-22 | 无锡市善源生物科技有限公司 | Method for preparing edible bacterial cellulose by using rice immersing water and product of method |
| CN112553010A (en) * | 2020-12-17 | 2021-03-26 | 聂绪明 | EM (effective microorganisms) bacterium laundry detergent and preparation method thereof |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5246848A (en) * | 1991-05-20 | 1993-09-21 | Olin Corporation | Process for providing enhanced yield and simplified isolation of hydrophobic enzymes from culture media |
| JP2002080288A (en) * | 2000-06-30 | 2002-03-19 | Asake Seiko Kk | Treatment method of garbage |
| CN1498961A (en) * | 2002-11-05 | 2004-05-26 | 张德怀 | Method for preparing culture fluid suitable to production of EM live bacterium |
-
2006
- 2006-07-27 CN CNB2006100197532A patent/CN100424165C/en active Active
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101591631B (en) * | 2009-06-08 | 2011-04-13 | 江西省天意生物技术开发有限公司 | EM original dew and production method thereof |
| CN103255074A (en) * | 2012-02-15 | 2013-08-21 | 何寒 | Simple and rapid propagation method of EM |
| CN103255059A (en) * | 2012-02-15 | 2013-08-21 | 何寒 | Preparation method of high purity EM protospecies |
| CN102911903A (en) * | 2012-10-29 | 2013-02-06 | 丘佩珍 | Cultivation of lactic acid bacteria and method of keeping nuisance-free rabbits with lactic acid bacteria |
| CN104531802A (en) * | 2014-12-11 | 2015-04-22 | 无锡市善源生物科技有限公司 | Method for preparing edible bacterial cellulose by using rice immersing water and product of method |
| CN112553010A (en) * | 2020-12-17 | 2021-03-26 | 聂绪明 | EM (effective microorganisms) bacterium laundry detergent and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100424165C (en) | 2008-10-08 |
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Effective date of registration: 20170714 Address after: 518052 Guangdong city of Shenzhen province Qianhai Shenzhen Hong Kong cooperation zone before Bay Road No. 1 building 201 room A (located in Shenzhen Qianhai business secretary Co. Ltd.) Patentee after: Zhongjian Biotechnology (Shenzhen) Co., Ltd. Address before: Nanning City People's Road West 530012 No. 6 the Guangxi Zhuang Autonomous Region second kindergarten King turn red Co-patentee before: Zhang Xuerong Patentee before: Zhou Malin |
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