CN1884561A - Process for preparing isomaltitol - Google Patents
Process for preparing isomaltitol Download PDFInfo
- Publication number
- CN1884561A CN1884561A CN 200510035439 CN200510035439A CN1884561A CN 1884561 A CN1884561 A CN 1884561A CN 200510035439 CN200510035439 CN 200510035439 CN 200510035439 A CN200510035439 A CN 200510035439A CN 1884561 A CN1884561 A CN 1884561A
- Authority
- CN
- China
- Prior art keywords
- yeast
- preparation
- palatinose
- carrier
- immobilization
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
This is the preparation method of isomalttulose. The steps includes: (1) the raw material is aqueous sucrose solutions, make it through the stablized alpha-glucosyltransferase reaction column or can to produce the transformation liquid which contains isomaltose and other sugers that transformed from enzyme; (2) make the prepared transformation liquid through de-sugers reactor with yeast immobilization in it, to get rid of the sugers other than isomaltose in transformation liquid; (3) then remove the residua in the acentric transformation liquid and get the clear liquid that contains isomaltose, then catalytic hydrogenation to produce isomalttulose. The content of isomaltose in the transformation liquid can reach more than 98%, then after catalytic hydrogenation, the obtained isomalttulose can increase the utilization rate of sucrose. Besides, it can also save energy and increase productivity.
Description
Technical field
The present invention relates to a kind of is the method for feedstock production hydroxyl isomaltulose with the sucrose solution.
Background technology
Hydroxyl isomaltulose (Palatinit) claim Parakin alditol again, is that the existing technology of feedstock production hydroxyl isomaltulose is as follows with the sucrose solution:
1, with the sucrose solution is raw material, make the conversion fluid of other the assorted sugar (as sucrose, glucose etc.) of sucrose solution by having the immobilized cell reaction column of α-Pu Taotang based transferase, make the Palatinose that forms by enzymatic conversion that contains about 85% and about 15%;
2, with prepared conversion fluid through concentrate, crystallization, isolate Palatinose crystallization and molasses (wherein still contain in the molasses Palatinose about 24% fail crystallization separate out);
3, the Palatinose crystallization that obtains is dissolved as Palatinose solution, again with this Palatinose solution by the reactor of catalyzer is housed, the hydrogenation pressurization, catalysis generates hydroxyl isomaltulose (this step is disclosed among the CN1317491A).
This technology is owing to still have Palatinose about 24% to fail crystallization to separate out in the molasses that produce, can't the hydrogenation utilization, can only sell as the low price byproduct, and make and produce hydroxyl isomaltulose and need consume more sucrose material, benefit is low.
Summary of the invention
The objective of the invention is to propose a kind ofly can improve the sucrose material utilization ratio, thereby improve the novel method of the preparation hydroxyl isomaltulose of productivity effect.
The preparation method of the hydroxyl isomaltulose that the present invention proposes, its technological process is as follows:
(1) is raw material with the sucrose solution, makes sucrose solution pass through immobilized α-Pu Taotang based transferase reaction column or jar, make and contain Palatinose and other the assorted sugared conversion fluids that forms by enzymatic conversion;
(2) prepared conversion fluid is fed the impurity elimination sugar retort that fixed yeast wherein is housed, to remove the assorted sugar beyond the Palatinose in the conversion fluid, described fixed yeast is formed by the yeast immobilization on carrier that can ferment and assimilate sucrose, glucose, trehalose and melizitose in the Saccharomycetaceae; Through test, cereuisiae fermentum in the Saccharomycetaceae (Saccharomyces cerevisiae), saccharomyces uvarum (Saccharomyces uvarum), unusual debaryomyces hansenii (Hansenula anomala), iron oxide red plum contract yeast (Mefschnikowia pulcherrima), red winter spore yeast (Rhodosporidium toruloides) and rhodotorula glutinis (Rhodotorola glutinis) fermentation and assimilation sucrose, glucose, trehalose and melizitose are better, can adopt in these yeast one or more to be blended in immobilization on the carrier and to be prepared into described fixed yeast;
(3) again with the centrifugal clear liquid that obtains containing Palatinose behind the residue of removing of the conversion fluid after the impurity elimination sugar, again this clear liquid shortening is made hydroxyl isomaltulose.
In preparation method of the present invention, described fixed yeast is to add sodium alginate in the yeast emulsion, the mass concentration that makes sodium alginate in the emulsion is 10～60g/L, carrier is immersed wherein, after the suspension carrier drips unnecessary emulsion, at massfraction is immobilization 1～2 hour in 0.1～1.0% calcium chloride solution, cleans to form.And the carrier of described fixed yeast can be strip or blocky nylon wire fabric or synthetic fabrics or other porous upholders, preferably adopt strip or blocky nylon wire fabric or synthetic fabrics, these strips or blocky nylon wire fabric or synthetic fabrics can fixedly hang on after carrying out the yeast immobilization among the impurity elimination sugar retort.
Described fixed yeast can adopt following concrete preparation technology:
A, barms is inserted in the fermentor tank of the bent juice of 5～12%Bx rice (or wort) fill sterilization, 28～32 ℃ of aerated culture 4～24 hours are cultivated when reaching 0.5～1.5 hundred million cells/ml, put jar;
B, fermented liquid are through the centrifugal yeast emulsion (by weight, containing stem cell 8～15%) that gets of yeast whizzer;
C, the yeast emulsion is mixed the mass concentration that makes sodium alginate in the emulsion with the sodium alginate underflow be 10～60g/L;
D, weave cotton cloth bar or cloth of nylon wire immersed in the above-mentioned underflow, hang on the support, drip and go unnecessary underflow;
E, weave cotton cloth bar or piece of the above-mentioned nylon wire that is soaked with underflow is hung in the immobilization jar, with massfraction is that the immersion of 0.1～1.0% calcium chloride solution made sodium alginate solidify in 1～2 hour, discharge calcium chloride solution then, inject the sterilization clear water and clean, standby 4～10 ℃ of refrigerations.Weave cotton cloth bar or piece of the fixed yeast nylon wire that immobilization is good hangs in the impurity elimination sugar retort during use.
In the novel preparation method of hydroxyl isomaltulose of the present invention, owing to have with the technological process of sucrose inversion liquid by impurity elimination sugar retort, conversion fluid is by utilizing particular fixed yeast Decompositions of Palatinose other assorted sugar (comprising trehalose, sucrose, glucose etc.) in addition in the impurity elimination sugar retort, Palatinose content in the conversion fluid is brought up to more than 98%, again this conversion fluid shortening is generated hydroxyl isomaltulose, improved the utilization ratio of sucrose material greatly; In addition, novel method saved in the existing technological process need with sucrose inversion liquid concentrate, crystallization, centrifugation molasses, produce the crystallization Palatinose, with operations such as crystallization Palatinose dissolvings, save energy is enhanced productivity significantly again.
Use novel method of the present invention, preparation sucrose material that hydroxyl isomaltulose consumed and existing technology relatively can be saved more than 20%.
Embodiment
Embodiment one
The technological process of preparation hydroxyl isomaltulose is as follows:
(1) will sterilize sucrose solution by immobilized α-Pu Taotang based transferase retort (immobilized alpha-glucosyl transferring enzyme enzyme pearl is housed in the retort), reaction makes the conversion fluid of other assorted sugar (as sucrose, glucose etc.) of the Palatinose that is formed by enzymatic conversion that contains about 85% and about 15%;
(2) conversion fluid with gained feeds the impurity elimination sugar retort that immobilization cereuisiae fermentum (Saccharomycescerevisiae) is housed under 25～32 ℃ of temperature, slowly stirred 30～60 minutes 28～32 ℃ of ventilations, do not show when bubble produces that to having assorted sugar is exhausted, obtain the conversion fluid of impurity elimination sugar;
(3) with the centrifugal clear liquid that removes to obtain containing behind the residue Palatinose of the conversion fluid after the impurity elimination sugar, this clear liquid contains Palatinose can reach 98%, again this clear liquid shortening is made hydroxyl isomaltulose.
Embodiment two
(1), contains 50 gram immobilization α-Pu Taotang based transferase enzyme pearls as the immobilized cell reaction column with 1000 milliliters of triangular flasks; 500 milliliters of the sucrose solutions of adding 50% (W/V) sterilization; 28～32 ℃ on bottle swingging machine, shake the bottle 1～2 hour; Remove by filter immobilization alpha-glucosyl transferring enzyme enzyme pearl and make the conversion fluid that contains the Palatinose that forms by enzymatic conversion and other assorted sugar;
(2) conversion fluid is put into the weave cotton cloth triangular flask (as impurity elimination sugar retort) of bar of the nylon wire that are equipped with 50 gram immobilization saccharomyces uvarums (Saccharomyces uvarum), slowly shook 30～60 minutes in 28～32 ℃, when not having bubble and produce, remove by filter the fixed yeast nylon wire piece of weaving cotton cloth, impurity elimination sugar conversion fluid;
(3) with the centrifugal clear liquid that removes to obtain containing behind the residue Palatinose of above-mentioned impurity elimination sugar conversion fluid, this clear liquid contains the Palatinose about 98%, again this clear liquid shortening is made hydroxyl isomaltulose.
Embodiment three
The step of the technological process of preparation hydroxyl isomaltulose is basic identical with embodiment one, and difference is in the sugared retort of the impurity elimination in second step unusual debaryomyces hansenii of immobilization (Hansenula anomala) to be housed.
Embodiment four
The step of the technological process of preparation hydroxyl isomaltulose is basic identical with embodiment one, and difference is in the sugared retort of the impurity elimination in second step immobilization iron oxide red plum contract yeast (Mefschnikowia pulcherrima) to be housed.
Embodiment five
The step of the technological process of preparation hydroxyl isomaltulose is basic identical with embodiment one, and difference is in the sugared retort of the impurity elimination in second step spore yeast of red winter of immobilization (Rhodosporidium toruloides) to be housed.
Embodiment six
The step of the technological process of preparation hydroxyl isomaltulose is basic identical with embodiment one, and difference is in the sugared retort of the impurity elimination in second step immobilization rhodotorula glutinis (Rhodotorola glutinis) to be housed.
Claims (4)
1, a kind of preparation method of hydroxyl isomaltulose, its technological process is as follows:
(1) is raw material with the sucrose solution, makes sucrose solution by having the immobilized cell reaction column of α-Pu Taotang based transferase, make the conversion fluid that contains the Palatinose that forms by enzymatic conversion and other assorted sugar;
(2) prepared conversion fluid is fed the impurity elimination sugar retort that fixed yeast wherein is housed, to remove the assorted sugar beyond the Palatinose in the conversion fluid, said fixed yeast is formed by the yeast immobilization on carrier that can ferment and assimilate sucrose, glucose, trehalose and melizitose in the Saccharomycetaceae;
(3) again with the centrifugal clear liquid that obtains containing Palatinose behind the residue of removing of the conversion fluid after the impurity elimination sugar, again this clear liquid shortening is made hydroxyl isomaltulose.
2, the preparation method of hydroxyl isomaltulose according to claim 1 is characterized in that described fixed yeast adopts in cereuisiae fermentum, saccharomyces uvarum, unusual debaryomyces hansenii, iron oxide red plum contract yeast, red winter spore yeast or the rhodotorula glutinis one or more to be blended in that immobilization forms on the carrier.
3, the preparation method of hydroxyl isomaltulose according to claim 1, it is characterized in that described fixed yeast is to add sodium alginate in the yeast emulsion, the mass concentration that makes sodium alginate in the emulsion is 10～60g/L, carrier is immersed wherein, after the suspension carrier drips unnecessary emulsion, at massfraction is immobilization 1～2 hour in 0.1～1.0% calcium chloride solution, cleans to form.
4, according to the preparation method of claim 1,2 or 3 described hydroxyl isomaltuloses, the carrier that it is characterized in that described fixed yeast is strip or blocky nylon wire fabric or synthetic fabrics.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100354399A CN100396780C (en) | 2005-06-22 | 2005-06-22 | Process for preparing isomaltitol |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100354399A CN100396780C (en) | 2005-06-22 | 2005-06-22 | Process for preparing isomaltitol |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1884561A true CN1884561A (en) | 2006-12-27 |
| CN100396780C CN100396780C (en) | 2008-06-25 |
Family
ID=37582867
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2005100354399A Active CN100396780C (en) | 2005-06-22 | 2005-06-22 | Process for preparing isomaltitol |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100396780C (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101200750B (en) * | 2007-11-29 | 2010-09-29 | 南京工业大学 | Rhubarb horsetails Erwinia sp. and its application in preparation of isomaltulose |
| CN101451156B (en) * | 2007-12-07 | 2011-11-30 | 山东福田药业有限公司 | Method for improving purity of xylose solution |
| CN102656176A (en) * | 2009-12-23 | 2012-09-05 | 赢创德固赛有限公司 | Sweetener and method for the production thereof |
| CN103549364A (en) * | 2013-11-01 | 2014-02-05 | 广西科学院生物研究所 | Functional food sweetening agent and preparation method thereof |
| CN105969824A (en) * | 2016-07-25 | 2016-09-28 | 山东百龙创园生物科技有限公司 | Preparation method of palatinitol |
| CN107712245A (en) * | 2017-11-22 | 2018-02-23 | 东莞亿智食品有限公司 | A kind of low sugar health care candy and preparation method thereof |
| CN111107753A (en) * | 2017-08-02 | 2020-05-05 | 赢创运营有限公司 | Isomaltulose-based sweeteners |
| CN112888697A (en) * | 2018-09-11 | 2021-06-01 | 祖德楚克尔股公司 | Improved method for producing sweetener |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0648992B2 (en) * | 1986-11-19 | 1994-06-29 | 三井製糖株式会社 | Separation method of sugar solution |
| CN1030530C (en) * | 1989-02-03 | 1995-12-20 | 广东省微生物研究所 | Preparation of parakin sugar by solidified alpha-glucosyl group transferase |
| CN1137127C (en) * | 2000-04-11 | 2004-02-04 | 广东省微生物研究所 | Process for preparing isomaltoketoseol from isomaltoketose |
-
2005
- 2005-06-22 CN CNB2005100354399A patent/CN100396780C/en active Active
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101200750B (en) * | 2007-11-29 | 2010-09-29 | 南京工业大学 | Rhubarb horsetails Erwinia sp. and its application in preparation of isomaltulose |
| CN101451156B (en) * | 2007-12-07 | 2011-11-30 | 山东福田药业有限公司 | Method for improving purity of xylose solution |
| CN102656176A (en) * | 2009-12-23 | 2012-09-05 | 赢创德固赛有限公司 | Sweetener and method for the production thereof |
| CN107048328A (en) * | 2009-12-23 | 2017-08-18 | 赢创德固赛有限公司 | Sweetener and preparation method thereof |
| CN107048328B (en) * | 2009-12-23 | 2021-07-09 | 赢创运营有限公司 | Sweetener and method for producing the same |
| CN103549364A (en) * | 2013-11-01 | 2014-02-05 | 广西科学院生物研究所 | Functional food sweetening agent and preparation method thereof |
| CN105969824A (en) * | 2016-07-25 | 2016-09-28 | 山东百龙创园生物科技有限公司 | Preparation method of palatinitol |
| CN111107753A (en) * | 2017-08-02 | 2020-05-05 | 赢创运营有限公司 | Isomaltulose-based sweeteners |
| CN107712245A (en) * | 2017-11-22 | 2018-02-23 | 东莞亿智食品有限公司 | A kind of low sugar health care candy and preparation method thereof |
| CN112888697A (en) * | 2018-09-11 | 2021-06-01 | 祖德楚克尔股公司 | Improved method for producing sweetener |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100396780C (en) | 2008-06-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1884561A (en) | Process for preparing isomaltitol | |
| CN102286454B (en) | Method for preparing isomaltulose by polyvinyl alcohol immobilized sucrose isomerase producing bacteria | |
| CN101255446B (en) | Method for continuous fermentation of glucose xylose by coupling immobilized yeast cell and pervaporation membrane | |
| DE102011100772A1 (en) | Process for the preparation of isomaltulose from plant juices | |
| CN106337065A (en) | Technological method for producing sodium gluconate by enzyme method | |
| Rafiqul et al. | A perspective bioproduction of xylitol by enzyme technology and future prospects. | |
| Zheng et al. | Scaling up of ethanol production from sugar molasses using yeast immobilized with alginate-based MCM-41 mesoporous zeolite composite carrier | |
| Gadow et al. | Cellulosic hydrogen production and microbial community characterization in hyper-thermophilic continuous bioreactor | |
| CN101921810B (en) | Method for preparing xylitol and L-arabinose mixed crystal from xylose mother liquid | |
| JP3594227B2 (en) | Production of fermented products | |
| US20120040423A1 (en) | Fermentation | |
| CN101165189A (en) | Meso one-step method ethanol production method for potato raw starch | |
| US20090130256A1 (en) | Decomposition of fermentation-inhibiting substances from a fluid | |
| CN1219782C (en) | Cane sugar material high-yield joint production process of crystalline fructose and manna sugar | |
| CN101906442B (en) | Method for producing alcohol by using industrial waste sweet water | |
| CN106318994B (en) | Method for preparing galactose derived from seaweed using agar hydrolase | |
| CN101343643B (en) | High production method for glycerol with multi-step zymotechnics | |
| CN102199641A (en) | Method for extracting sugar from enzymic hydrolysate of jerusalem artichoke residues | |
| CN1086202C (en) | Technological method for producing alcohol by high-effective fermentation with waste molasses used as raw material | |
| Enebo | On the formation of reducing sugars in thermophilic cellulose fermentation | |
| JPH0198500A (en) | Method for refining sucrose solution | |
| CN101525637A (en) | Method for cleanly producing potato ethanol with low viscosity | |
| CN107164416B (en) | Method for producing succinic acid by fermenting actinobacillus succinogenes fixed by polypropylene non-woven fabric | |
| SU492541A1 (en) | The method of preparation of yeast for champagne | |
| UTAMI et al. | Production and immobilization pectinase from Bacillus sp. 2P11 using alginate beads |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: LIFECOME BIOCHEMISTRY CO., LTD. Free format text: FORMER OWNER: WANDAI BIOTECHNOLOGY (SHENZHEN) CO., LTD. Effective date: 20130326 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 518057 SHENZHEN, GUANGDONG PROVINCE TO: 353400 NANPING, FUJIAN PROVINCE |
|
| TR01 | Transfer of patent right |
Effective date of registration: 20130326 Address after: Nanping City, Fujian province Nanpu 353400 Pucheng County eco industrial park No. 19 Patentee after: Lifecome Biochemistry Co., Ltd. Address before: 518057 R2-A521 room, South District, Shenzhen hi tech Industrial Zone, Guangdong, China Patentee before: Million generation Biotechnology (Shenzhen) Co., Ltd. |