CN1873001A - Method for procducing great cattles through transgene cloning ferritin gene of human milk - Google Patents
Method for procducing great cattles through transgene cloning ferritin gene of human milk Download PDFInfo
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- CN1873001A CN1873001A CN 200610076240 CN200610076240A CN1873001A CN 1873001 A CN1873001 A CN 1873001A CN 200610076240 CN200610076240 CN 200610076240 CN 200610076240 A CN200610076240 A CN 200610076240A CN 1873001 A CN1873001 A CN 1873001A
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- human lactoferrin
- lactoferrin
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Images
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Transgenic host | hLF sequence | Promoter | rhLF Expressing level | Published year & author |
Fungi(Aspergillus nidulans) Fungi(Aspergillus awamori) Yeast (Saccharomyces cerevisiae) Baby-hamster kidney cells Tobacco cells (Nicotiana tabacum L.) Tobacco Plant Potato plant | 2.3kb cDNA 2.1kb cDNA Full length cDNA 2.3kb full length cDNA Full length cDNA 2.1kb full length cDNA 2.3kb full length cDNA | 300bp A.nidulans alcA promoter 1.1kb glucoamylase gene promoter Yeast chelatin promoter MT-1 promoter CaMV 35s promoter DP35ScaMV promoter 1.MasP2 promoter 2.CaMV 35s | 5μg/ml(total) 1.5μg/ml(secreted) (ELISA analysis)1 2,000μg/ml (secreted) (ELISA analysis) 20-30μg/ml(total) 1.5-2.0μg/ml (secreted)2 20μg/ml(secreted) (Determined by the A280 of the pooled column fractions) 0.6-2.5%of the total cellular protein (1)0.1% of total extracted leaf proteins (2)0.3% of total extracted leaf proteins3 (ELISA) (1)0.01% of total soluble protein (2)0.1% of total | 1992,Pauline P. Ward,et al 1995,Pauline P. Ward,et al 1993,Qianwa Liang,et al 1991,Stowell K.M.,et al 1994,Amitava Mitra,et al 1998,Valérie Salmon,et al. 2000,Daniel K.X. et al |
Mice Mice Mice Mice Mice Cattle | Full length cDNA Genomic sequence 1.hLF cDNA 2.hLF genomic sequence 2.5kb hLF cDNA 27kb genomic sequence Genomic sequence | promoter 7.7kb αs1-casein promoter 6.2kb αs1-casein gene promoter αs1-casein gene promoter 2kb bovine β-casein gene promoter 10kb bovine β-casein gene promoter 6.2kb αs1-casein gene promoter | soluble protein4 (ELISA analysis) 0.1-36μg/ml (RIA analysis) >1,000μg/ml 400-1,300μg/ml 300-3,800μg/ml (RIA analysis)6 1-200μg/ml&7 (ELISA analysis) 60-6,600μg/ml8 (ELISA analysis) 300-2,800μg/ml9 (ELISA analysis) | 1994,Gerard J.Platenburg,et al 1993,De Wit, I.C.M,et al 1997,Jan H. Nuijens,et al. 1994,1997,Sun Jung Kim et al 1999,Sun Jung Kim,et al 2002,Patrick H.C.Van Berkel |
Composition | Final concentration (mM) | Add-on g/100ml |
NaCl KCl NaHCO 3 Na Pyruvate Phenol Red | 114.7 3.1 26.2 20.4 1μl/ml | 0.67 0.023 0.22 0.002 100μl |
The primer name | Primer sequence | Product length (bp) | Annealing temperature (℃) |
hLF5’PF | TGCTTTGTTTGTATTGAGGGTC | 906 | 60 |
hLF5’PR | CCAGGAACAAACTTACGGAG | ||
hLFPF | AGGACACAGACAGCAGACAC | 503 | 60 |
hLFPF | CAGCCCTCTTCTTCCTCTTC | ||
hLF3’PF | TTCCTTCCACCACTGTTGAG | 633 | 60 |
hLF3’PR | CAAATACCTCTGCCGCTGTT |
The primer name | Primer sequence | Product length (bp) | Annealing temperature (℃) |
hLF5’PF | TGCTTTGTTTGTATTGAGGGTC | 906 | 60 |
hLF5’PR | CCAGGAACAAACTTACGGAG | ||
hLFPF | AGGACACAGACAGCAGACAC | 503 | 60 |
hLFPF | CAGCCCTCTTCTTCCTCTTC |
hLF3’PF | TTCCTTCCACCACTGTTGAG | 633 | 60 |
hLF3’PR | CAAATACCTCTGCCGCTGTT |
Claims (10)
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CN 200510066116 CN1687425A (en) | 2005-04-21 | 2005-04-21 | Bioreactor of animal mammary gland for producing recombined human milk ferritin-method of transgene cloning great cattle for human milk ferritin |
CN200510066116.6 | 2005-04-21 | ||
CNB2006100762405A CN100469876C (en) | 2005-04-21 | 2006-04-21 | Method for procducing great cattles through transgene cloning ferritin gene of human milk |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102102126A (en) * | 2010-12-07 | 2011-06-22 | 华中农业大学 | Quick, simple and convenient detection method for transgenic cows with human lactoferrin gene |
WO2014005466A1 (en) * | 2012-07-02 | 2014-01-09 | 北京济福霖生物技术有限公司 | Product for mitigating iron deficiency anemia and method for preparing same |
CN113278625A (en) * | 2021-03-12 | 2021-08-20 | 新疆农垦科学院 | Gene sequence for cultivating specific IGF1 transgenic sheep and identification primer and identification method thereof |
-
2006
- 2006-04-21 CN CNB2006100762405A patent/CN100469876C/en active Active
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102102126A (en) * | 2010-12-07 | 2011-06-22 | 华中农业大学 | Quick, simple and convenient detection method for transgenic cows with human lactoferrin gene |
CN102102126B (en) * | 2010-12-07 | 2013-02-13 | 华中农业大学 | Quick, simple and convenient detection method for transgenic cows with human lactoferrin gene |
WO2014005466A1 (en) * | 2012-07-02 | 2014-01-09 | 北京济福霖生物技术有限公司 | Product for mitigating iron deficiency anemia and method for preparing same |
CN113278625A (en) * | 2021-03-12 | 2021-08-20 | 新疆农垦科学院 | Gene sequence for cultivating specific IGF1 transgenic sheep and identification primer and identification method thereof |
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