CN1860887A - Pesticide of ricinine - Google Patents
Pesticide of ricinine Download PDFInfo
- Publication number
- CN1860887A CN1860887A CN 200610035170 CN200610035170A CN1860887A CN 1860887 A CN1860887 A CN 1860887A CN 200610035170 CN200610035170 CN 200610035170 CN 200610035170 A CN200610035170 A CN 200610035170A CN 1860887 A CN1860887 A CN 1860887A
- Authority
- CN
- China
- Prior art keywords
- pesticide
- ricinine
- age
- diamond
- back moth
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Catching Or Destruction (AREA)
Abstract
An insecticide for killing plutella xylostella and noctuid is prepared from ricinine, alpha-cypermethrin, tween-80, piperonyl butoxide and water through proportional mixing.
Description
Technical field
The present invention relates to field of biological pesticide, be specifically related to a kind of pesticide of ricinine.
Background technology
Secondly castor-oil plant is Euphorbiaceae Ricinus plant, and largest production state is Brazil, be China and Thailand, and present whole world castor-oil plant annual production is surplus in the of 90 ten thousand tons.The main component castor oil of castor-oil plant is widely used in chemical industry, weaving, light industry, medical department production coating, paint, soap, printing ink, dyeing auxiliaries, rate accelerating material, emulsifier etc.Contain multiple toxin in the castor bean seed, as ricin, recinine, allergen, hemagglutinin etc.The existence of these toxin has hindered the application of castor cake on feed industry that contains 35～40% protein, causes the waste of a large amount of protein.People have carried out a large amount of research to the mechanism of action and the application of these toxic components in the toxic component of trying every possible means to remove in the castor cake now, and ricin has been widely used for preparing antibody targeted antineoplastic (immunotoxin).
Recinine (Picinine) chemical name 3-cyanogen-4-methoxyl group-2-pyridone, molecular formula is C
8H
8N
2O
2, molecular weight 161.4.Recinine is white needles or column crystal, and 201.5 ℃ of fusing points are 170 ℃～180 ℃ distillations in 20 seconds.Be soluble in hot water and chloroform, certain solvability is also arranged, but solvability be very little in ether, benzinum and benzene in hot ethanol.It is a kind of alkaloid, and not with sour salify, its alkaline solution can make the potassium permanganate reduction, generates hydrogen cyanide simultaneously.Because of containing cyano group in the molecule, so toxicity is very big.Recinine is present in the cauline leaf and seed of castor-oil plant.
In existing agricultural chemicals, though biopesticide has been arranged, main still based on chemical pesticide, the residual and environmental pollution that the excessive use of chemical pesticide causes.If can be developed to the toxic component in the castor-oil plant biopesticide of high-efficiency low-toxicity, then both can remove the toxic component in the castor cake, improve the comprehensive utilization ratio of castor-oil plant, can prevent and treat pest again, bigger economic benefit and social benefit are arranged.
Summary of the invention
The object of the present invention is to provide a kind of pesticide of ricinine,, improve the comprehensive utilization ratio of castor-oil plant, and benefit agricultural production the toxic component recinine in the castor-oil plant is developed to the biopesticide of high-efficiency low-toxicity.
Pesticide of ricinine prescription of the present invention is as follows: (percetage by weight)
Recinine 0.5-2%
Efficient cis-, trans-cypermethrin 0.1-2%
Tween 80 0.02-0.20%
Butacide 0.09-0.2%
Water surplus
Each component is stirred evenly promptly by the proportioning mixing.
Beneficial effect of the present invention: pesticide of ricinine of the present invention is to the good disinsection effect of little noctuid of twill and diamond-back moth, and is lower as vegetation water toxicity, biological degradation easily.
Embodiment
The preparation of embodiment 1 pesticide of ricinine
Recinine 0.6%, efficient cis-, trans-cypermethrin 0.2%, Tween 80 0.15%, Butacide 0.2%, water are surplus, each component are mixed stirring evenly according to above-mentioned percentage by weight, are made into pesticide of ricinine.
The preparation of embodiment 2 pesticide of ricinine
Recinine 2%, efficient cis-, trans-cypermethrin 1.8%, Tween 80 0.02%, Butacide 0.1%, water are surplus, each component are mixed stirring evenly according to above-mentioned percentage by weight, are made into pesticide of ricinine.
The insecticidal test of embodiment 3 pesticide of ricinine
Utilize the pesticide of ricinine of embodiment 1 preparation to do following insecticidal test.
Carry out preliminary experiment earlier, the concentration range according to the consequence devised reagent agent of preliminary experiment is made into required gradient concentration.Little noctuid of twill and diamond-back moth are respectively established 5 concentration, and establish the clear water contrast respectively, and each concentration is established 3 repetitions.Adopt the culture dish of diameter 9cm, filter paper on the pad in each culture dish is according to the about 15cm of the different placements of examination worm
2Fresh blade, wherein prodenia litura is a taro leaves for the examination blade, diamond-back moth is the heart of a cabbage leaf for the examination blade, inserts the examination worm then.Utilize the Potter spray tower to carry out two-sided quantitative spraying, handle soup 1ml for every, spray time 1min puts into constant incubator (25 ℃ of temperature, humidity 85%) after the spraying.Investigate, write down examination worm death condition respectively after handling 24h, 48h, 72h, 96h, and in time replenish fresh blade.
Table 1 pesticide of ricinine is to the indoor virulence regression equation of prodenia litura 2 instar larvaes
| Processing time | Regression equation (Y=a+bx) | LC 50(mg/L) | Correlation coefficient (R) | 95% confidence limit (mg/L) | LC 95(mg/L) |
| 96h | Y＝-1.9302+1.8261x | 6239.74 | 0.9740 | 4528.18～8598.24 | 49654.56 |
Table 2 pesticide of ricinine is to the indoor virulence regression equation of diamond-back moth 2 instar larvaes
| During processing | Regression equation | LC 50(mg/L) | Correlation coefficient | 95% confidence limit (mg/L) | LC 95(mg/L) |
| Between | (Y＝a+bx) | (R) | |||
| 96h | Y＝0.3790+1.0963x | 16401.19 | 0.9724 | 10043.15～26783.80 | 519070.42 |
Table 3 pesticide of ricinine is to the toxicity test result of prodenia litura 2 instar larvaes
| For examination concentration (ml/L) | Repeat | Total borer population | Handle back 96h | ||
| Dead borer population | Lethality % | Corrected mortality | |||
| 2500 | 1 | 10 | 2 | 20 | 21.82％ |
| 2 | 11 | 2 | 18.18 | ||
| 3 | 11 | 3 | 27.27 | ||
| On average | 21.82 | ||||
| 5000 | 1 | 10 | 4 | 40 | 36.67％ |
| 2 | 10 | 3 | 30 | ||
| 3 | 10 | 4 | 40 | ||
| On average | 36.67 | ||||
| 10000 | 1 | 10 | 8 | 80 | 75.93％ |
| 2 | 9 | 7 | 77.78 | ||
| 3 | 10 | 7 | 70 | ||
| On average | 75.93 | ||||
| 20000 | 1 | 10 | 8 | 80 | 83.33％ |
| 2 | 10 | 9 | 90 | ||
| 3 | 30 | 8 | 80 | ||
| On average | 83.33 | ||||
| 40000 | 1 | 11 | 10 | 90.91 | 90.60％ |
| 2 | 11 | 10 | 90.91 | ||
| 3 | 10 | 9 | 90 | ||
| On average | 90.60 | ||||
| CK (clear water) | 1 | 10 | 0 | 0 | |
| 2 | 10 | 0 | 0 | ||
| 3 | 10 | 0 | 0 | ||
| On average | |||||
Table 4 recinine is to the toxicity test result of diamond-back moth 2 instar larvaes
| For examination concentration (ml/L) | Repeat | Total borer population | Handle back 96h | ||
| Dead borer population | Lethality % | Corrected mortality | |||
| 2500 | 1 | 10 | 2 | 20 | 19.35％ |
| 2 | 11 | 3 | 27.27 | ||
| 3 | 11 | 2 | 18.18 | ||
| On average | 21.82 | ||||
| 5000 | 1 | 10 | 3 | 30 | 27.74％ |
| 2 | 10 | 3 | 30 | ||
| 3 | 10 | 3 | 30 | ||
| On average | 30 | ||||
| 10000 | 1 | 10 | 4 | 40 | 41.51％ |
| 2 | 10 | 5 | 50 | ||
| 3 | 10 | 4 | 40 | ||
| On average | 43.33 | ||||
| 20000 | 1 | 10 | 5 | 80 | 50.17％ |
| 2 | 10 | 5 | 50 | ||
| 3 | 9 | 5 | 50 | ||
| On average | 51.85 | ||||
| 40000 | 1 | 11 | 8 | 72.73 | 68.72％ |
| 2 | 11 | 7 | 63.64 | ||
| 3 | 11 | 8 | 72.73 | ||
| On average | 69.7 | ||||
| CK (clear water) | 1 | 11 | 1 | 9.09 | |
| 2 | 11 | 0 | 0 | ||
| 3 | 10 | 0 | 0 | ||
| On average | 3.03 |
In the Toxicity Determination process of pesticide of ricinine to prodenia litura 2 instar larvaes and diamond-back moth, every 24h investigation once tries the death condition of worm, and the lethality of each concentration 24h, 48h, 72h is all smaller after finding to handle, and reaches than high mortality behind the processing 96h.By table 3, the data of table 4 show that pesticide of ricinine all has certain cytotoxicity to prodenia litura and diamond-back moth, and from regression equation, correlation coefficient reaches 0.9470 and 0.9427 respectively, shows good relationship.
Embodiment 4 field insecticidal tests (this is operated in Fanyu, Guangzhou institute of agricultural sciences and finishes)
Utilize the pesticide of ricinine of embodiment 1 preparation to do following insecticidal test.
1, dispenser object: the diamond-back moth on the broccoli
2, handle: 25 times of pesticide of ricinine dilutions; 50 times of pesticide of ricinine dilutions.
3, test method:
Three repetitions are established in this test.The sub-district randomized arrangement, each handles the fix a point insect population number of diamond-back moth above the investigation of 10 strain broccoli of sub-district, investigates 1-2 age, 3-4 age, three worm attitudes of pupa respectively.Investigate 5 sprays in insect density afternoon medicine morning October 18, spray value is to be sprayed onto blade to drip and be standard, respectively at the insect density of October 21,24 days, three investigation on November 4 diamond-back moth, adopts the worm rate methods analyst control efficiency that goes down behind the spray medicine.
4, result of the test:
By table 6 as seen, 25 times and 50 times two processing of pesticide of ricinine all have obvious control efficiency to 1-2 diamond-back moth in age, the spray medicine after 3 days 1-2 diamond-back moth in age just begun to go down, reached the peak of going down on the 6th day, and 25 times of processing to handle preventive effects than 50 times good; 25 times of processing of pesticide of ricinine also have certain preventive effect to 3-4 diamond-back moth in age, and 50 times of processing are not obvious to 3-4 diamond-back moth in age preventive effect; Pesticide of ricinine does not almost have effect to the pupa of diamond-back moth.CK handles undesirable to the diamond-back moth control efficiency.Three processing all are that the 6th day preventive effect is best behind the fountain medicine, and preventive effect significantly reduced in the 17 day.
In addition we find that also 25 times of pesticide of ricinine and 50 times of two processing are fine to the prodenia litura control efficiency in 1-2 age during the process of the investigation.
5, significance of difference analysis:
Because October, the preventive effect of three processing on the 24th all reached peak, so analyze the significance of difference of three processing with total worm attitude insect population rate that goes down on October 24.25 times of pesticide of ricinine are remarkable with contrast difference; 50 times of pesticide of ricinine are not remarkable with contrast difference.
Insect population is counted statistical form before and after table 5 dispenser: (unit: head)
| The spray medicine phase | (10.18 before the spray medicine) | (10.21 spray medicine after 3 days) | ||||||
| 1-2 age | 3-4 age | Pupa | Total worm attitude | 1-2 age | 3-4 age | Pupa | Total worm attitude | |
| 25 times | 248 | 243 | 36 | 527 | 64 | 298 | 20 | 382 |
| 50 times | 147 | 90 | 12 | 249 | 52 | 233 | 9 | 294 |
| CK (clear water) | 96 | 79 | 11 | 186 | 40 | 250 | 21 | 311 |
Insect population is counted statistical form before and after table 6 dispenser: (unit: head)
| The spray medicine phase | (10.24 spray medicine after 6 days) | (11.4 spray medicine after 17 days) | ||||||
| 1-2 age | 3-4 age | Pupa | Total mouthful attitude | 1-2 age | 3-4 age | Pupa | Total worm attitude | |
| 25 times | 37 | 101 | 56 | 194 | 152 | 237 | 36 | 425 |
| 50 times | 35 | 99 | 63 | 197 | 68 | 193 | 32 | 293 |
| CK (clear water) | 12 | 98 | 63 | 173 | 131 | 208 | 50 | 389 |
Insect population after table 7 dispenser rate that goes down: (unit: %)
| Date | 10.21 | 10.24 | 11.4 | |||||||||
| Handle | 1-2 age | 3-4 age | Pupa | Total worm attitude | 1-2 age | 3-4 age | Pupa | Total worm attitude | 1-2 age | 3-4 age | Pupa | Total worm attitude |
| 25 times | 74.19 | -22.63 | 44.44 | 27.51 | 85.08 | 58.44 | -55.56 | 63.19 | 38.71 | 2.47 | 0 | 19.35 |
| 50 times | 64.63 | -158.89 | 25.00 | -18.07 | 76.19 | -10.00 | -425.00 | 20.88 | 53.74 | 1.10 | -166.67 | -17.67 |
| CK | 58.33 | -216.46 | -90.91 | -67.20 | 87.50 | -24.05 | -472.33 | 6.99 | -36.46 | -163.29 | -354.55 | -10.91 |
Table 8 is proofreaied and correct the insect population rate that goes down: (unit: %)
| Date | 10.21 | 10.24 | 11.4 | |||||||||
| Handle | 1-2 age | 3-4 age | Pupa | Total worm attitude | 1-2 age | 3-4 age | Pupa | Total worm attitude | 1-2 age | 3-4 age | Pupa | Total worm attitude |
| 25 times | 38.06 | 61.25 | 70.90 | 56.64 | -19.69 | 66.50 | 72.82 | 60.42 | 55.09 | 62.96 | 78.00 | 27.28 |
| 50 times | 15.12 | 18.19 | 60.71 | 29.38 | -90.48 | 11.33 | 8.34 | 14.93 | 66.10 | 62.44 | 41.33 | -6.10 |
Table 9 total worm attitude insect population on October 24 rate difference significance analysis that goes down
| Handle | Total worm attitude insect population rate (%) that goes down | LSD |
| 25 times | 63.19 | Significantly |
| 50 times | 20.88 | Not remarkable |
| CK | 6.99 |
Claims (1)
1. pesticide of ricinine is characterized in that being made up of following component and percetage by weight:
Recinine 0.5-2%
Efficient cis-, trans-cypermethrin 0.1-2%
Tween 80 0.02-0.20%
Butacide 0.2-0.09%
Water surplus
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610035170 CN1860887A (en) | 2006-04-25 | 2006-04-25 | Pesticide of ricinine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610035170 CN1860887A (en) | 2006-04-25 | 2006-04-25 | Pesticide of ricinine |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1860887A true CN1860887A (en) | 2006-11-15 |
Family
ID=37388495
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200610035170 Pending CN1860887A (en) | 2006-04-25 | 2006-04-25 | Pesticide of ricinine |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1860887A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107347877A (en) * | 2016-05-09 | 2017-11-17 | 中国科学院大学 | A kind of block copolymer containing PEG-PCL Tetranychus cinnabarinus inhibitor and preparation method thereof |
| CN113367165A (en) * | 2018-03-02 | 2021-09-10 | 中国农业科学院棉花研究所 | Method for extracting castor-containing insecticidal active substance and combined application of castor-containing insecticidal active substance and pyrethroid insecticide |
-
2006
- 2006-04-25 CN CN 200610035170 patent/CN1860887A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107347877A (en) * | 2016-05-09 | 2017-11-17 | 中国科学院大学 | A kind of block copolymer containing PEG-PCL Tetranychus cinnabarinus inhibitor and preparation method thereof |
| CN107347877B (en) * | 2016-05-09 | 2020-12-01 | 中国科学院大学 | Tetranychus cinnabarinus inhibitor containing block copolymer PEG-PCL and preparation method thereof |
| CN113367165A (en) * | 2018-03-02 | 2021-09-10 | 中国农业科学院棉花研究所 | Method for extracting castor-containing insecticidal active substance and combined application of castor-containing insecticidal active substance and pyrethroid insecticide |
| CN113498794A (en) * | 2018-03-02 | 2021-10-15 | 中国农业科学院棉花研究所 | Method for extracting castor-containing insecticidal active substance and combined application of castor-containing insecticidal active substance and carbamate insecticide |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| van den Brink et al. | Effects of chronic low concentrations of the pesticides chlorpyrifos and atrazine in indoor freshwater microcosms | |
| CN102273440A (en) | Ultralow volume liquid containing clothianidin | |
| CN112930115B (en) | Liquid preparation containing biphenyl compounds and application thereof | |
| CN101066055A (en) | Pesticide composition containing emamectin benzoate | |
| CN108782546A (en) | A kind of bucket mixes agricultural chemicals synergist and preparation method thereof | |
| CN102217601A (en) | Highly efficient and environmentally protective pesticidal composition | |
| CN102246799A (en) | Insecticidal composition containing spinetoram | |
| CN1860887A (en) | Pesticide of ricinine | |
| CN114287450A (en) | Pesticide composition for preventing and treating sugarcane thrips | |
| CN102047915B (en) | Insecticidal composition containing thiacloprid amide | |
| CN103315006A (en) | Insecticidal composition containing natural celastrus angulatus and natural vitex negundo and application thereof | |
| Van Straalen et al. | Effects of benzo (a) pyrene on food assimilation and growth efficiency in Porcellio scaber(Isopoda). | |
| CN102224825A (en) | Pesticide composition containing sulfoxaflor and nereistoxin compound | |
| CN103858920A (en) | Spinetoram-containing insecticidal composition | |
| Prudic et al. | Isolation, identification, and quantification of potential defensive compounds in the Viceroy butterfly and its larval host–plant, Carolina willow | |
| CN102165954A (en) | Efficient environment-friendly pesticide composition containing biopesticide | |
| CN101411341A (en) | Pokeberry extract and novel use thereof | |
| CN102657213A (en) | Ultra-low volume liquor containing metaflumizone and esbiothrin pesticides | |
| CN102228028A (en) | Pesticide composition containing sulfur-fluorine oxime ether and part of amide pesticides | |
| CN1751572A (en) | Composition of miticide | |
| CN106561718A (en) | Method for green and organic prevention and control of pieris rapae in facility vegetable greenhouse | |
| Achonduh et al. | Susceptibility status of Anopheles gambiae sl (Diptera: Culicidae) from cabbage growing areas associated with pyrethroid and organophosphate use in Accra, Ghana | |
| CN102246759A (en) | Pesticide composition containing flonicamid and spinosad | |
| CN118120756B (en) | Composition for preventing and controlling rice diseases and insect pests | |
| CN1316890C (en) | Composite agro-chemicals of hexaflumuron and pyridaphenthion |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |