CN1837460A - Method for quickly extracting bacteria cellulose from mother liquor - Google Patents
Method for quickly extracting bacteria cellulose from mother liquor Download PDFInfo
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- CN1837460A CN1837460A CN 200610026001 CN200610026001A CN1837460A CN 1837460 A CN1837460 A CN 1837460A CN 200610026001 CN200610026001 CN 200610026001 CN 200610026001 A CN200610026001 A CN 200610026001A CN 1837460 A CN1837460 A CN 1837460A
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- bacteria cellulose
- acid solution
- cellulose
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Abstract
This invention discloses a process for the separation and purification of bacteria cellulose from mother liquid, which comprises the following steps: putting wet bacteria cellulose film into distilled water, formulating 1-9% alkali liquor and 1-10% acid solution by concentration,separetly holding temperature to 20-60 degrees with digital display thermostatic and magnetic stirrer; putting wet bacteria cellulose film into alkali liquor formulated for swelling for 3-10min, then taking it out and putting it into acid solution formulated, steeping it for 4-8min with magnetic stirrer whose revolution rate is 100- 700rpm; taking the wet bacteria cellulose film and rinsing it for 2-3 times, then conserving it in fridge. The invention comprises the following good effects: (1) using not only alkali liquor, but also acid solution; (2) the temperature isn't high and the reaction time isn't long in purification process; (3) the solution used is nonvolatile, so the process is nontoxic; (4) the solution can be used repeatedly, so the extraction cycle is shortened.
Description
Technical field
The present invention relates to a kind of method that the bacteria cellulose that directly takes out is purified fast from liquid fermentation medium, relate in particular to and a kind ofly respectively cellulose wet-coating is carried out the processing of short time, obtain that outward appearance is pure white and translucent, a kind of method of bacterial cellulose wet-coating that better purity is arranged with alkali lye and inorganic acid solution.
Background technology
Bacteria cellulose is the fibrid element by microorganisms, and acetic acid bacteria produces bacteria cellulose, helps the vital movement of himself, and in liquid environment, the formation of cellulose surface liquid film can help the acetic acid bacteria thalline to maintain the sufficient liquid level growth of oxygen.The biosynthesis of bacteria cellulose can be divided into polymerization, secretion, assembling and crystallization four big processes, and this four big process is highly coupling, and closely related with the specific site on the cell membrane.People such as Ross find that the cellulose that A.xylinum produces not is a part that forms cell membrane, but are secreted into outside the thalline by the cell membrane micropore, and the secretion process of bacteria cellulose follows its biosynthesis to carry out simultaneously.Growth along with thalline, the bacteria cellulose of about 12 to 70 molecules is secreted into the nutrient solution from the micropore of the at interval about 10nm of cell surface simultaneously, interconnect by hydrogen bond at these cellulosic molecules of cell surface, form cellulosic fibril, these fibrils not only can with cell embedding wherein and also the eyed structure of bacteria cellulose fibril also be convenient to the filling of other thalline or non-cellulose secretion, these impurity are all influential to the tensile strength of cellulose membrane, Young's modulus, degree of crystallinity, permeability etc.The analysis of pure bacteria cellulose X-diffraction has shown that bacteria cellulose has the crystal structure of height rule.Thereby obtain highly purified bacterial cellulose wet-coating the application that bacteria cellulose from now on does technological layer is again had positive role.
According to bibliographical information, the method for extracting bacteria cellulose at present is to boil in 100 ℃ in alkali lye mostly, or earlier centrifugally boils in alkali lye again, and the time of these operations mostly needs just can obtain comparatively pure bacteria cellulose more than one hour.Yet because the long-time immersion of alkali lye and boil unavoidably and can the surface texture of bacteria cellulose be damaged, thereby bacteria cellulose film is produced a series of harmful effects.
Summary of the invention
Technical problem to be solved by this invention provides a kind of bacteria cellulose method for quickly extracting from mother liquor, to remedy the deficiencies in the prior art or defective, meets the demands of production and living.
In order to solve the problems of the technologies described above, the technical solution adopted in the present invention is a kind of bacteria cellulose method for quickly extracting from mother liquor, comprises the steps:
(1) bacterial cellulose wet-coating is placed distilled water, compound concentration is 1～9% aqueous slkali and 1～10% acid solution, respectively constant temperature to 20～60 ℃ under the effect of digital display constant temperature blender with magnetic force;
(2) bacterial cellulose wet-coating is placed the alkali lye of being joined, swelling 3～10min takes out, and puts into the acid solution of being prepared, and at rotating speed is to soak 4～8min under the effect of 100～700rpm magnetic stirring apparatus;
(3) take out cellulose wet-coating flushing 2～3 times, put in the refrigerator and preserve.
As optimized technical scheme: the bacterial classification of described product bacteria cellulose comprises acetobacter xylinum (Acetobacter xylinum), produce acetobacter (A.acetigenum), acetify bacillus (Acetobacteraceti), Pasteur's acetobacter (Acetobacterpasteurianus), glucose bacillus (Gluconobaterspp), Agrobacterium (Agrobaterium tumefaciens), rhizobium (Rhizobium trifolii), sarcine (Sarcina ventriculi), Pseudomonas cepacia (Seudomonas cepacia), Pseudomonas cocovenenans (P.cocovenenans), in the campylobacter jejuni (Campylobater jejuni) one or more;
Described aqueous slkali comprises NaOH solution, KOH solution, ammonia spirit, NaHCO
3In the solution one or more; Described acid solution comprises H
2SO
4Solution, HCl solution, CH
3In the COOH solution one or more; Described aqueous slkali and acid solution are reusable.
Principle of the present invention is bacterial cellulose wet-coating first swelling under the effect of higher temperature alkali lye, zymotic fluid, protein, fat, thalline and the bacterium secretion that wraps up in the netted structure of cellulose or have can overflow or is loosening, then use in the inorganic acid and alkali lye that the wet film surface is excessive, under the effect of centrifugal stirring, make impurity break away from film body and float on liquid level.
The invention has the beneficial effects as follows:
(1) solution is not to use alkali lye merely, also has acid solution, and the alkali lye that mid-term, carried on the cellulose membrane surface can neutralize purifying.
(2) solution temperature is not high in the purification process, the reaction time is not long, can not cause big harm to cellulosic structure.
(3) owing to use solution non-volatility, avirulence, thereby can not work the mischief to environment and operating personnel.
(4) solution after the operation is reusable again through simple adjusting with the PH meter, has shortened extracting cycle.
The specific embodiment
Below in conjunction with specific embodiment the present invention is further elaborated.
Embodiment 1
The bacterial cellulose wet-coating that acetobacter xylinum is produced places distilled water, joins concentration and be 5% NaOH solution and 9% H
2SO
4Solution, constant temperature to 40 ℃ under the effect of digital display constant temperature blender with magnetic force places NaOH solution with bacterial cellulose wet-coating respectively, takes out behind the swelling 5min, and alkali lye can keep repeatedly and uses; Then bacteria cellulose being put into temperature is that 40 ℃, concentration are 9% H
2SO
4In the solution, open the speed governing button rotating speed is transferred to 500rpm work 5min, cellulose its inner loosening impurity in the process of rotation enters solution under action of centrifugal force; Take out cellulose wet-coating and wash 2 times as for preserving in the refrigerator, acid solution can be adjusted repeated use a little.
Embodiment 2
The bacterial cellulose wet-coating that the glucose bacillus is produced places distilled water, joins concentration and be 1% NaOH solution and 1% H
2SO
4Solution, constant temperature to 60 ℃ under the effect of digital display constant temperature blender with magnetic force places NaOH solution with bacterial cellulose wet-coating respectively, takes out behind the swelling 3min, and alkali lye can keep repeatedly and uses; Then bacteria cellulose being put into temperature is that 45 ℃, concentration are 8% H
2SO
4In the solution, open the speed governing button rotating speed is transferred to 700rpm work 4min, cellulose its inner loosening impurity in the process of rotation enters solution under action of centrifugal force; Take out cellulose wet-coating and wash 3 times as for preserving in the refrigerator, acid solution can be adjusted repeated use a little.
Embodiment 3
The bacterial cellulose wet-coating that rhizobium are produced places distilled water, joins concentration and be 9% NaOH solution and 10% H
2SO
4Solution, constant temperature to 20 ℃ under the effect of digital display constant temperature blender with magnetic force places NaOH solution with bacterial cellulose wet-coating respectively, takes out behind the swelling 10min, and alkali lye can keep repeatedly and uses; Then bacteria cellulose being put into temperature is that 40 ℃, concentration are 9% H
2SO
4In the solution, open the speed governing button rotating speed is transferred to 100rpm work 8min, cellulose its inner loosening impurity in the process of rotation enters solution under action of centrifugal force; Take out cellulose wet-coating and wash 2 times as for preserving in the refrigerator, acid solution can be adjusted repeated use a little.
Through chemical analysis, the purity of bacteria cellulose reaches 99.1%; The alkali lye and the acid solution that reclaim are adjusted back repetition aforesaid operations a little, find that the bacteria cellulose purity after new the purification is 97.5%, still have higher purity, promptly the repeated use of alkali lye and inorganic acid is very little to the influence of bacteria cellulose purification process.
Claims (5)
1, a kind of bacteria cellulose method for quickly extracting from mother liquor is characterized in that comprising the steps:
(1) bacterial cellulose wet-coating is placed distilled water, compound concentration is 1～9% aqueous slkali and 1～10% acid solution, respectively constant temperature to 20～60 ℃ under the effect of digital display constant temperature blender with magnetic force;
(2) bacterial cellulose wet-coating is placed the alkali lye of being joined, swelling 3～10min takes out, and puts into the acid solution of being prepared, and at rotating speed is to soak 4～8min under the effect of 100～700rpm magnetic stirring apparatus;
(3) take out cellulose wet-coating flushing 2～3 times, put in the refrigerator and preserve.
2, method of purification according to claim 1 is characterized in that: the bacterial classification of described product bacteria cellulose comprises acetobacter xylinum, produces acetobacter, acetify bacillus, Pasteur's acetobacter, glucose bacillus, Agrobacterium, rhizobium, sarcine, Pseudomonas cepacia, Pseudomonas cocovenenans, campylobacter jejuni one or more.
3, method of purification according to claim 1 is characterized in that: described aqueous slkali comprises NaOH solution, KOH solution, ammonia spirit, NaHCO
3Solution one or more.
4, method of purification according to claim 1 is characterized in that: described acid solution comprises H
2SO
4Solution, HCl solution, CH
3In the COOH solution one or more.
5, method of purification according to claim 1 is characterized in that: described aqueous slkali and acid solution are reusable.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100260019A CN100386478C (en) | 2006-04-25 | 2006-04-25 | Method for quickly extracting bacteria cellulose from mother liquor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100260019A CN100386478C (en) | 2006-04-25 | 2006-04-25 | Method for quickly extracting bacteria cellulose from mother liquor |
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| Publication Number | Publication Date |
|---|---|
| CN1837460A true CN1837460A (en) | 2006-09-27 |
| CN100386478C CN100386478C (en) | 2008-05-07 |
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ID=37014960
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2006100260019A Expired - Fee Related CN100386478C (en) | 2006-04-25 | 2006-04-25 | Method for quickly extracting bacteria cellulose from mother liquor |
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| CN (1) | CN100386478C (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102178639A (en) * | 2011-04-29 | 2011-09-14 | 钟春燕 | Preparation method of spot-lightening wet mask |
| CN102516576A (en) * | 2011-12-09 | 2012-06-27 | 海南椰国食品有限公司 | Method for purifying biological cellulose membrane product |
| CN105926050A (en) * | 2016-05-23 | 2016-09-07 | 东华大学 | Macroscopic fibers based on directional arrangement of bacterial cellulose nanofibers and preparation method of macroscopic fibers |
| CN108532343A (en) * | 2018-04-20 | 2018-09-14 | 北京理工大学珠海学院 | A kind of method for purifying and separating of bacteria cellulose |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07268128A (en) * | 1994-03-31 | 1995-10-17 | Fujitsuko Kk | Dried material of cellulose derived from microorganism and its production |
| JP4061661B2 (en) * | 1996-05-24 | 2008-03-19 | 味の素株式会社 | Method for treating bacterial cellulose concentrate |
| DE10022751C2 (en) * | 2000-03-10 | 2002-04-18 | Fzmb Forschungszentrum Fuer Me | Process for the production of specific moldings or layers from bacterial cellulose |
| US20050037082A1 (en) * | 2003-08-13 | 2005-02-17 | Wan-Kei Wan | Poly(vinyl alcohol)-bacterial cellulose nanocomposite |
| CN1330254C (en) * | 2005-04-30 | 2007-08-08 | 海南大学 | Production method of bacterial cellulose gel for dish |
-
2006
- 2006-04-25 CN CNB2006100260019A patent/CN100386478C/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102178639A (en) * | 2011-04-29 | 2011-09-14 | 钟春燕 | Preparation method of spot-lightening wet mask |
| CN102516576A (en) * | 2011-12-09 | 2012-06-27 | 海南椰国食品有限公司 | Method for purifying biological cellulose membrane product |
| CN102516576B (en) * | 2011-12-09 | 2013-12-04 | 海南椰国食品有限公司 | Method for purifying biological cellulose membrane product |
| CN105926050A (en) * | 2016-05-23 | 2016-09-07 | 东华大学 | Macroscopic fibers based on directional arrangement of bacterial cellulose nanofibers and preparation method of macroscopic fibers |
| CN105926050B (en) * | 2016-05-23 | 2018-07-17 | 东华大学 | The macroscopic fibres and preparation method thereof aligned based on bacteria cellulose nanofiber |
| CN108532343A (en) * | 2018-04-20 | 2018-09-14 | 北京理工大学珠海学院 | A kind of method for purifying and separating of bacteria cellulose |
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|---|---|
| CN100386478C (en) | 2008-05-07 |
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Effective date of registration: 20090410 Address after: No. 2999, Renmin North Road, Xincheng District, Shanghai, Songjiang: 201620 Co-patentee after: Shanghai Ronggang Industrial and Trading Ltd. Patentee after: Donghua University Address before: No. 2999, Renmin North Road, Xincheng District, Shanghai, Songjiang: 201620 Patentee before: Donghua University |
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Owner name: SHANGHAI YIFANG AGRICULTURAL TECHNOLOGY CO., LTD. Free format text: FORMER OWNER: SHANGHAI RONGGANG INDUSTRIAL AND TRADING CO., LTD. |
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Effective date of registration: 20110616 Address after: 201620 No. 2999 North Renmin Road, Xincheng District, Shanghai, Songjiang Co-patentee after: ShangHai YiFang Rural Technology Holdings Co.,Ltd. Patentee after: Donghua University Address before: 201620 No. 2999 North Renmin Road, Xincheng District, Shanghai, Songjiang Co-patentee before: Shanghai Ronggang Industrial and Trading Ltd. Patentee before: Donghua University |
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