CN1826527B - 基于酰基载体蛋白的蛋白标记方法 - Google Patents

基于酰基载体蛋白的蛋白标记方法 Download PDF

Info

Publication number
CN1826527B
CN1826527B CN2004800209859A CN200480020985A CN1826527B CN 1826527 B CN1826527 B CN 1826527B CN 2004800209859 A CN2004800209859 A CN 2004800209859A CN 200480020985 A CN200480020985 A CN 200480020985A CN 1826527 B CN1826527 B CN 1826527B
Authority
CN
China
Prior art keywords
protein
acp
fusion
label
albumen
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN2004800209859A
Other languages
English (en)
Chinese (zh)
Other versions
CN1826527A (zh
Inventor
K·约翰森
N·乔治
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ecole Polytechnique Federale de Lausanne EPFL
Original Assignee
Ecole Polytechnique Federale de Lausanne EPFL
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ecole Polytechnique Federale de Lausanne EPFL filed Critical Ecole Polytechnique Federale de Lausanne EPFL
Publication of CN1826527A publication Critical patent/CN1826527A/zh
Application granted granted Critical
Publication of CN1826527B publication Critical patent/CN1826527B/zh
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C07K2319/43—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a FLAG-tag

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
CN2004800209859A 2003-05-23 2004-05-19 基于酰基载体蛋白的蛋白标记方法 Expired - Fee Related CN1826527B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP03405364.5 2003-05-23
EP03405364 2003-05-23
PCT/IB2004/001733 WO2004104588A1 (en) 2003-05-23 2004-05-19 Methods for protein labeling based on acyl carrier protein

Publications (2)

Publication Number Publication Date
CN1826527A CN1826527A (zh) 2006-08-30
CN1826527B true CN1826527B (zh) 2010-11-24

Family

ID=33462272

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2004800209859A Expired - Fee Related CN1826527B (zh) 2003-05-23 2004-05-19 基于酰基载体蛋白的蛋白标记方法

Country Status (10)

Country Link
US (2) US7666612B2 (https=)
EP (1) EP1627226A1 (https=)
JP (1) JP2007509608A (https=)
KR (1) KR20060015612A (https=)
CN (1) CN1826527B (https=)
AU (1) AU2004242249A1 (https=)
CA (1) CA2526579A1 (https=)
NZ (1) NZ543653A (https=)
WO (1) WO2004104588A1 (https=)
ZA (1) ZA200509454B (https=)

Families Citing this family (89)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1720332A (zh) * 2002-10-03 2006-01-11 洛桑生态综合技术联合公司 O6－烷基鸟嘌呤－dna烷基转移酶的底物
CA2553261C (en) 2004-01-16 2014-03-18 Stefan Barth Immunokinases
EP2351839A3 (en) * 2004-07-02 2011-10-05 AVI BioPharma, Inc. Antisense antibacterial method and compound
US8067571B2 (en) 2005-07-13 2011-11-29 Avi Biopharma, Inc. Antibacterial antisense oligonucleotide and method
EP1800695A1 (en) 2005-12-21 2007-06-27 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Immuno-RNA-constructs
CA2693155A1 (en) * 2007-07-25 2009-01-29 Fraunhofer Gesellschaft Zur Forderung Der Angewandten Forschung E.V. Self coupling recombinant antibody fusion proteins
US20110014216A1 (en) * 2007-10-03 2011-01-20 Covalys Biosciences Ag Drug Transfer Based on Coenzyme A and Acyl Carrier Protein
ATE522810T1 (de) * 2007-10-29 2011-09-15 New England Biolabs Inc Verfahren und zusammensetzungen zum nachweis und zur anreicherung kleiner ziel-rnas
EP2093564A1 (en) 2008-02-25 2009-08-26 Technische Universität Dresden Medizinische Fakultät Carl Gustav Carus Method for distinguishing secretory granules of different ages
WO2009092808A1 (en) * 2008-01-24 2009-07-30 National University Of Ireland, Maynooth Method for detecting phosphopantetheinyl transferase activity
US9085798B2 (en) 2009-04-30 2015-07-21 Prognosys Biosciences, Inc. Nucleic acid constructs and methods of use
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
WO2011127099A1 (en) 2010-04-05 2011-10-13 Prognosys Biosciences, Inc. Spatially encoded biological assays
KR20200133284A (ko) 2010-05-28 2020-11-26 사렙타 쎄러퓨틱스, 인코퍼레이티드 변형된 서브유니트간 결합 및/또는 말단 그룹을 갖는 올리고뉴클레오타이드 유사체
JP2013534525A (ja) * 2010-06-23 2013-09-05 ヴィヴォスクリプト，インコーポレイテッド 心臓疾患の治療のために遺伝子改変を伴わずに細胞を再プログラミングするための組成物および方法
US10017763B2 (en) 2010-09-03 2018-07-10 Sarepta Therapeutics, Inc. dsRNA molecules comprising oligonucleotide analogs having modified intersubunit linkages and/or terminal groups
EP2694709B1 (en) 2011-04-08 2016-09-14 Prognosys Biosciences, Inc. Peptide constructs and assay systems
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
HK1201514A1 (en) 2011-11-18 2015-09-04 Sarepta Therapeutics, Inc. Functionally-modified oligonucleotides and subunits thereof
EP2782916B1 (en) 2011-11-21 2018-02-28 Promega Corporation Carboxy x rhodamine analogs
US9267117B2 (en) 2012-03-15 2016-02-23 New England Biolabs, Inc. Mapping cytosine modifications
CN104411831B (zh) 2012-03-20 2020-08-11 萨勒普塔医疗公司 寡核苷酸类似物的硼酸缀合物
EP2855520B1 (en) 2012-06-04 2018-09-26 Novartis AG Site-specific labeling methods and molecules produced thereby
WO2014043561A1 (en) * 2012-09-14 2014-03-20 The Regents Of The University Of California Reversible chemoenzymatic labeling of native and fusion carrier protein motifs
EP4592400A3 (en) 2012-10-17 2025-10-29 10x Genomics Sweden AB Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
EP3736573A1 (en) 2013-03-15 2020-11-11 Prognosys Biosciences, Inc. Methods for detecting peptide/mhc/tcr binding
WO2014152298A1 (en) 2013-03-15 2014-09-25 Promega Corporation New silicon and germanium dyes for use in genetic identity
US9708658B2 (en) 2013-03-19 2017-07-18 New England Biolabs, Inc. Enrichment of target sequences
CA2916662C (en) 2013-06-25 2022-03-08 Prognosys Biosciences, Inc. Methods and systems for determining spatial patterns of biological targets in a sample
US10288608B2 (en) 2013-11-08 2019-05-14 Prognosys Biosciences, Inc. Polynucleotide conjugates and methods for analyte detection
WO2016093838A1 (en) 2014-12-11 2016-06-16 New England Biolabs, Inc. Enrichment of target sequences
CN104531725B (zh) * 2014-12-23 2017-11-03 华南农业大学 一种可被4’磷酸泛酰巯基乙胺修饰的序列及其用于固定蛋白质的方法
EP3901282B1 (en) 2015-04-10 2023-06-28 Spatial Transcriptomics AB Spatially distinguished, multiplex nucleic acid analysis of biological specimens
US11020417B2 (en) 2015-06-04 2021-06-01 Sarepta Therapeutics, Inc Methods and compounds for treatment of lymphocyte-related diseases and conditions
CN108101977A (zh) * 2018-01-17 2018-06-01 天津市湖滨盘古基因科学发展有限公司 一种人的acp蛋白突变蛋白及其应用
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
WO2020123316A2 (en) 2018-12-10 2020-06-18 10X Genomics, Inc. Methods for determining a location of a biological analyte in a biological sample
US12529094B2 (en) 2018-12-10 2026-01-20 10X Genomics, Inc. Imaging system hardware
CN109485655B (zh) * 2018-12-20 2021-06-25 河北百灵威超精细材料有限公司 一种生物素马来酰亚胺的制备方法
JP7627910B2 (ja) * 2018-12-21 2025-02-07 フエー・イー・ベー・フエー・ゼツト・ウエー サイトカイン及び足場タンパク質を含む融合タンパク質
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
CN111748089B (zh) * 2019-03-28 2023-07-18 成都先导药物开发股份有限公司 一种生物素标记化合物以及确定化合物结合靶标蛋白的方法
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
WO2021092433A2 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Enhancing specificity of analyte binding
EP4081656A1 (en) 2019-12-23 2022-11-02 10X Genomics, Inc. Compositions and methods for using fixed biological samples in partition-based assays
CN115135984B (zh) 2019-12-23 2025-12-23 10X基因组学有限公司 可逆固定试剂及其使用方法
ES2982420T3 (es) 2019-12-23 2024-10-16 10X Genomics Inc Métodos para el análisis espacial mediante el uso de la ligazón con plantilla de ARN
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
WO2021158925A1 (en) 2020-02-07 2021-08-12 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
WO2021183921A1 (en) 2020-03-12 2021-09-16 New England Biolabs, Inc. A rapid diagnostic test for lamp
EP4139485B1 (en) 2020-04-22 2023-09-06 10X Genomics, Inc. Methods for spatial analysis using targeted rna depletion
EP4153776B1 (en) 2020-05-22 2025-03-05 10X Genomics, Inc. Spatial analysis to detect sequence variants
EP4414459B1 (en) 2020-05-22 2025-09-03 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
EP4421186B1 (en) 2020-06-08 2025-08-13 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
EP4165207B1 (en) 2020-06-10 2024-09-25 10X Genomics, Inc. Methods for determining a location of an analyte in a biological sample
ES2994976T3 (en) 2020-06-25 2025-02-05 10X Genomics Inc Spatial analysis of dna methylation
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
EP4110945A2 (en) 2020-08-21 2023-01-04 New England Biolabs, Inc. A rapid diagnostic test for lamp
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
EP4729631A2 (en) 2020-12-21 2026-04-22 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
EP4305196B1 (en) 2021-04-14 2025-04-02 10X Genomics, Inc. Methods of measuring mislocalization of an analyte
EP4320271B1 (en) 2021-05-06 2025-03-19 10X Genomics, Inc. Methods for increasing resolution of spatial analysis
WO2022256503A1 (en) 2021-06-03 2022-12-08 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
WO2023034489A1 (en) 2021-09-01 2023-03-09 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
WO2023086880A1 (en) 2021-11-10 2023-05-19 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
WO2023102118A2 (en) 2021-12-01 2023-06-08 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
WO2023197015A1 (en) 2022-04-08 2023-10-12 New England Biolabs, Inc. Compositions and analysis of dephosphorylated oligoribonucleotides
EP4482979B1 (en) 2022-11-09 2025-10-01 10X Genomics, Inc. Methods, compositions, and kits for determining the location of multiple analytes in a biological sample
WO2025129193A1 (en) 2023-12-15 2025-06-19 New England Biolabs, Inc. Glycosyltransferases and related methods
GB202400129D0 (en) 2024-01-04 2024-02-21 Cambridge Entpr Ltd Nucleic acid barcodes
CN117903960B (zh) * 2024-03-15 2024-06-04 东北林业大学 一种产大麻二酚酸的重组酿酒酵母菌株及其构建方法与应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN87106119A (zh) * 1986-07-31 1988-04-27 加利福尼亚基因公司 酰基载体蛋白-dna序列及合成
US5087563A (en) * 1990-03-09 1992-02-11 The United States Of America, As Represented By The Secretary Of Agriculture Acyl carrier protein-I/protein-A gene fusion, products and methods
US20020132308A1 (en) * 2000-08-24 2002-09-19 Mpep @ Page 300-M Novel constructs and their use in metabolic pathway engineering

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2232230C (en) 1995-10-13 2005-06-28 Ralph H. Lambalot Phosphopantetheinyl transferases and uses thereof
DE60212642T2 (de) * 2001-04-10 2007-06-14 ECOLE POLYTECHNIQUE FéDéRALE DE LAUSANNE Verfahren zur verwendung von o6-alkylguanin-dna-alkyltransferasen

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN87106119A (zh) * 1986-07-31 1988-04-27 加利福尼亚基因公司 酰基载体蛋白-dna序列及合成
US5087563A (en) * 1990-03-09 1992-02-11 The United States Of America, As Represented By The Secretary Of Agriculture Acyl carrier protein-I/protein-A gene fusion, products and methods
US20020132308A1 (en) * 2000-08-24 2002-09-19 Mpep @ Page 300-M Novel constructs and their use in metabolic pathway engineering

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Mohammad Reza Mofid, Robert Finking et al.Recognition of Hybridyl Carrier Proteins/Acyl Carrier Proteinsin Nonribosomal Peptide Synthetase Modules by the4`-Phophopantetheinyl Transferases AcpS and Sfp.The Journal of Biological Chemistry277 19.2002,277(19),17023-17031.
Mohammad Reza Mofid, Robert Finking et al.Recognition of Hybridyl Carrier Proteins/Acyl Carrier Proteinsin Nonribosomal Peptide Synthetase Modules by the4`-Phophopantetheinyl Transferases AcpS and Sfp.The Journal of Biological Chemistry277 19.2002,277(19),17023-17031. *
T. Ritsema, A.M.Gehring, A.R.Stuitje et al.Functional analysis of an interspecies chimera of acylcarrierproteins indicates a specialized domain forproteinrecognition.Mol Gen Genet257.1998,257641-648. *
T.Ritsema A.M.Gehring

Also Published As

Publication number Publication date
US8476031B2 (en) 2013-07-02
US20100173384A1 (en) 2010-07-08
CN1826527A (zh) 2006-08-30
JP2007509608A (ja) 2007-04-19
KR20060015612A (ko) 2006-02-17
EP1627226A1 (en) 2006-02-22
ZA200509454B (en) 2007-05-30
AU2004242249A1 (en) 2004-12-02
US20070082336A1 (en) 2007-04-12
CA2526579A1 (en) 2004-12-02
NZ543653A (en) 2008-10-31
US7666612B2 (en) 2010-02-23
WO2004104588A1 (en) 2004-12-02

Similar Documents

Publication Publication Date Title
CN1826527B (zh) 基于酰基载体蛋白的蛋白标记方法
AU2002251257B2 (en) Methods of Using O6-alkylguanine-DNA Alkyltransferases
AU2002251257A1 (en) Methods of Using O6-alkylguanine-DNA Alkyltransferases
WO2019241438A2 (en) Multipartite luciferase
Kaddoum et al. One-step split GFP staining for sensitive protein detection and localization in mammalian cells
JP2004532028A5 (https=)
Huang et al. The cysteine‐free single mutant C32S of APEX2 is a highly expressed and active fusion tag for proximity labeling applications
Robson et al. A large conformational change couples the ATP binding site of SecA to the SecY protein channel
KR20210093890A (ko) 유비퀴틴 결찰효소 작용제의 스크리닝 방법
Kindermann et al. Synthesis and characterization of bifunctional probes for the specific labeling of fusion proteins
CA2503228A1 (en) Ip3 protein binding assay
Agalarov et al. Ribosomal protein S1 induces a conformational change of the 30S ribosomal subunit
Lai et al. Environment‐sensitive Fluorescent Turn‐on Chemical Probe for the Specific Detection of O‐Methylguanine‐DNA Methyltransferase (MGMT) in Living Cells
Huynh Subunit assembly of the sodium, potassium-ATPase
HK1094908A (en) Methods for protein labeling based on acyl carrier protein

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
REG Reference to a national code

Ref country code: HK

Ref legal event code: DE

Ref document number: 1094908

Country of ref document: HK

C14 Grant of patent or utility model
GR01 Patent grant
REG Reference to a national code

Ref country code: HK

Ref legal event code: WD

Ref document number: 1094908

Country of ref document: HK

CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20101124

Termination date: 20170519

CF01 Termination of patent right due to non-payment of annual fee