CN1817263A - Souvenir containing DNA and production thereof - Google Patents
Souvenir containing DNA and production thereof Download PDFInfo
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- CN1817263A CN1817263A CN 200610009826 CN200610009826A CN1817263A CN 1817263 A CN1817263 A CN 1817263A CN 200610009826 CN200610009826 CN 200610009826 CN 200610009826 A CN200610009826 A CN 200610009826A CN 1817263 A CN1817263 A CN 1817263A
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- dna
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Abstract
A souvenir containing DNA is composed of a full-sealed container and the resin bead or glass bead in which the DNA is adsorbed. Its preparing process includes such steps as extracting DNA from a human body, adsorbing the DNA by resin bead or glass bead, loading the resin bead or glass bead in said container, sealing and vacuumizing.
Description
Technical field
The present invention relates to the manufacturing process of souvenir and this DNA souvenir of a kind of DNA of including.
Background technology
The Human Genome Project be with Manhattan atomic plan, Apollo Moon-landing Project and human sciences's history of claiming on Important Project.It is formally started October nineteen ninety by U.S. government, and the scientist of virtuous, day of back, English, method, medium 6 countries successively becomes a full member of, and 16 laboratories and 1100 bioscience men, computer elite and technical staff's participations are arranged.This is planned to draw out human genome in 2003 and " finishes figure ".Human genome is the summation that human cell's nuclear staining body carries the gene of all inhereditary materials.Chromosome is to be entwined by double-helical DNA (DNA), and DNA is the material of constitutivegene.6,000,000 cells are arranged in the human body, a nuclear is all arranged in each cell, chromosome promptly is positioned at nucleus.The chromosome of human body has 23 to (promptly 46), every pair all be half heredity from the own father, half heredity is from breeder mother.Contain and contain human genome in the nucleus, genetic fragment can marking protein, and cell is different and be in different developmental phases and caused the protein difference.In other words, all body cells of a people contain identical genome, but each cell is expressed different protein according to environment, and osteocyte produces bone development proteins associated matter, and muscle cell is that muscle is produced protein.Owing to contain the natural mutation of recessive gene and gene in the gene, even the people of present medical diagnosis health also might carry recessive gene pathogenic, that disable; Moreover even complete healthy people's gene also natural mutation may take place, this sudden change may be that what to cause a disease also may be non-pathogenic.Getting up so extract people's genome and preservation, is significant to everyone, and the Study on Genome that also is beneficial to man.
Disclose " ornaments of a kind of DNA of including and manufacturing process thereof " among the ZL02120544.2, there are the following problems for this technology: (1) experimental procedure is loaded down with trivial details; (2) contain albumen among the DNA, purity is not high; (3) DNA hardly as seen.
Summary of the invention
At prior art have that experimental procedure is loaded down with trivial details, contain that albumen, purity are not high among the DNA, DNA visible defects hardly, the invention provides souvenir and the manufacture method thereof of a kind of DNA of including.
The souvenir of the DNA of including of the present invention is a full hermetic container, and the glue pearl or the bead of adsorption of DNA is housed in the inner chamber of described full hermetic container.Its manufacture method is: a, extract DNA according to conventional method from human body; B, in dna solution, add 0.1～0.4ml lauryl sodium sulfate and 0.2～2ml glue pearl or bead, reversing mixing 5～6 minutes, 9000～14000r/min rotating speed is centrifugal 30～60 seconds under the room temperature, removes supernatant; Add ethanol and fully mix, 9000～14000r/min rotating speed is centrifugal 30～60 seconds under the room temperature, removes supernatant, adds ethanol again and fully mixes, and 9000～14000r/min rotating speed is centrifugal 1～5 minute under the room temperature, removes supernatant; At this moment DNA is adsorbed on glue pearl or the bead, then glue pearl or bead is suspended in the DNA storage liquid, and preserves standby in 4 ℃ environment; C, the glue pearl or the bead that will be adsorbed with DNA are put into container, and sealing is isolated with air.
The extracting method of genomic DNA is a lot, and extraction characteristics of the present invention are: 1. can the rapid extraction genomic DNA; 2. genomic DNA purity height; DNA is adsorbed on visible glass pellet or the glue pearl; 4. genomic DNA is stored in and is difficult for degraded in the storage liquid and 5. can makes and carry the genomic DNA material and dye multiple color.The present invention has that experimental procedure is terse, DNA purity height, DNA are adsorbed on the bead, knows that its position, carrier and storage liquid all can dye, and the advantage of long preservation at room temperature.
The specific embodiment
The specific embodiment one: the souvenir that includes DNA of present embodiment is a full hermetic container, and the glue pearl or the bead of adsorption of DNA is housed in the inner chamber of described full hermetic container.
The inner chamber of full hermetic container described in the present embodiment is the transparent body.Described full hermetic container can be the pendant of ornaments such as crystal, headwear, wrist-watch, brooch, ring, earrings or necklace, or neonate's souvenir or forefathers' board etc.
The specific embodiment two: present embodiment includes the souvenir of DNA: a, extracts DNA according to conventional method from human body according to following method manufacturing; B, in dna solution, add 0.1～0.4ml lauryl sodium sulfate and 0.2～2ml glue pearl or bead, reversing mixing 5～6 minutes, 9000～14000r/min rotating speed is centrifugal 30～60 seconds under the room temperature, removes supernatant; Add ethanol and fully mix, 9000～14000r/min rotating speed is centrifugal 30～60 seconds under the room temperature, removes supernatant, adds ethanol again and fully mixes, and 9000～14000r/min rotating speed is centrifugal 1～5 minute under the room temperature, removes supernatant; At this moment DNA is adsorbed on glue pearl or the bead, then glue pearl or bead is suspended in the DNA storage liquid, and preserves standby in 4 ℃ environment; C, the glue pearl or the bead that will be adsorbed with DNA are put into container, and sealing is isolated with air.
In the present embodiment, described DNA storage liquid is ethanol or organic hydrocarbon.Described DNA derives from blood of human body, hair or intraoral cast-off cells.The adsorbance of DNA is 1～100 μ g on described glue pearl or the bead.The concrete operations step of described c step is as follows: take a clean container, use the alcohol washing container, again with the washing of DNA storage liquid; Put into the glue pearl or the bead that are adsorbed with DNA, in container, add the DNA storage liquid then, sealing, isolated with air.
The specific embodiment three: present embodiment includes the souvenir of DNA according to following method manufacturing:
1, the venous blood 0.1～5ml that extracts human body makes blood sample, and is standby;
2, prepare and take following reagent:
A, 4～10% (w/v) ethylenediamine tetra-acetic acid diformazan salt;
B, TNM:10mM trishydroxymethylaminomethane-hydrochloric acid (pH=7.6), 10mM sodium chloride, 5mM magnesium chloride;
C, TE:10mM trishydroxymethylaminomethane-hydrochloric acid (pH=8.0), 1mM ethylenediamine tetra-acetic acid diformazan salt (pH=8.0);
D, 20mg/ml Proteinase K;
E, 0.2% (w/v) lauryl sodium sulfate;
F, 10% (w/v) lauryl sodium sulfate;
G, 1ml glue pearl or bead;
H, 70% (v/v) ethanol.
3, select equipment for use: water bath, ice machine, centrifuge;
4, technical process:
1. from blood, extract DNA:
Blood collection, 0.1～5ml whole blood is put into the test tube of 0.07ml 4% ethylenediamine tetra-acetic acid diformazan salt, get after shaking up and wherein partly put into centrifuge tube, add the TNM of 3 times of volumes, mix centrifugal, under 4 ℃ of temperature with the centrifugal 15min of 2500r/min rotating speed, remove supernatant, in this precipitation, add the TNM of 1 times of volume, precipitation is suspended, with the centrifugal 15min of 2500r/min rotating speed, remove supernatant under 4 ℃ of temperature; After this adding 10% lauryl sodium sulfate, 0.015～0.1ml, TE 0.1～0.5ml and Proteinase K 5～10ul, fully put into 55 ℃ of water-baths 0.5 hour behind the mixing, is limpid liquid until solution; In solution, add 0.2ml0.2% lauryl sodium sulfate and 1ml glue pearl or bead, reversing mixing 5～6 minutes, under the room temperature 12, centrifugal 30 seconds of 000r/min rotating speed is removed supernatant; Add 70% ethanol and fully mix, under the room temperature 12, centrifugal 30 seconds of 000r/min rotating speed is removed supernatant, adds 70% ethanol again and fully mixes, and under the room temperature 12, centrifugal 3 minutes of 000r/min rotating speed is removed supernatant; At this moment DNA promptly is adsorbed on glue pearl or the bead, glue pearl or bead is suspended in the storage liquid, and preserves standby in 4 ℃ environment.
2. the DNA that extracts is deposited in souvenir;
Take a clean container, the alcohol with 70% is washing container more than once, is no less than 3 times with the washing of DNA storage liquid again; Put into the glue pearl or the bead that are adsorbed with DNA, in container, add the DNA storage liquid then, sealing, isolated with air.
The specific embodiment four: present embodiment includes the souvenir of DNA according to following method manufacturing:
1, get 1～10 hair, standby;
2, prepare and take following reagent:
A, lysate S:100ug/ml Proteinase K, 100mM trishydroxymethylaminomethane-hydrochloric acid, 0.2～2% (w/v) lauryl sodium sulfate, 5mM ethylenediamine tetra-acetic acid diformazan salt, 4mM dithiothreitol (DTT), 2mM sodium chloride;
B, 0.2% (w/v) lauryl sodium sulfate;
C, 1ml glue pearl or bead;
D, 70% ethanol;
3, select equipment for use: water bath, ice machine, centrifuge;
4, technical process:
1. from hair, extract DNA:
Get 1～10 hair, cut hair near-end 3mm and put into centrifuge tube, add 0.5ml lysate S, put into 55 ℃ of water-baths 5～10 hours; In solution, add 0.2ml 0.2% lauryl sodium sulfate and 1ml glue pearl or bead, reversing mixing 5～6 minutes, under the room temperature 12, centrifugal 30 seconds of 000r/min rotating speed is removed supernatant; Add 70% ethanol and fully mix, under the room temperature 12, centrifugal 30 seconds of 000r/min rotating speed is removed supernatant, adds 70% ethanol again and fully mixes, and under the room temperature 12, centrifugal 3 minutes of 000r/min rotating speed is removed supernatant; At this moment DNA promptly is adsorbed on glue pearl or the bead, glue pearl or bead is suspended in the storage liquid, and preserves standby in 4 ℃ environment.
2. the DNA that extracts is deposited in souvenir;
Take a clean container, the alcohol with 70% is washing container more than once, is no less than 3 times with the washing of DNA storage liquid again; Put into the glue pearl or the bead that are adsorbed with DNA, in container, add the DNA storage liquid then, sealing, isolated with air.
The specific embodiment five: present embodiment includes the souvenir of DNA according to following method manufacturing:
1, wipe 1～40 time in the inboard, oral cavity with medical cotton stick, make the cotton swab air dry, in the clean container of packing into, standby;
2, prepare and take following reagent:
A, physiological saline: 0.9% (w/v) sodium chloride;
B, 10% (w/v) lauryl sodium sulfate;
C, TE:10mM trishydroxymethylaminomethane-hydrochloric acid (pH=8.0), 1mM ethylenediamine tetra-acetic acid diformazan salt (pH=8.0);
D, 20mg/ml Proteinase K;
E, 0.2% (w/v) lauryl sodium sulfate;
F, 1ml glue pearl or bead;
G, 70% ethanol;
3, select equipment for use: water bath, ice machine, centrifuge;
4, technical process:
1. from the oral cavity, extract DNA:
With medical cotton stick in the oral cavity inboard the wiping 1～40 time, cotton swab is put into 5～10ml physiological saline, room temperature 1～5 minute is taken out cotton swab and is made it be with moisture less, under 4 ℃ of temperature with the rotating speed of 9000r/min centrifugal 5 minutes, removes supernatant; After this adding 10% lauryl sodium sulfate, 0.015～0.1ml, TE 0.1～0.5ml and Proteinase K 5～10ul, fully put into 55 ℃ of water-baths 0.5 hour behind the mixing, is limpid liquid until solution; In solution, add 0.2ml 0.2% lauryl sodium sulfate and 1ml glue pearl or bead, reversing mixing 5～6 minutes, under the room temperature 12, centrifugal 30 seconds of 000r/min rotating speed is removed supernatant; Add 70% ethanol and fully mix, under the room temperature 12, centrifugal 30 seconds of 000r/min rotating speed is removed supernatant, adds 70% ethanol again and fully mixes, and under the room temperature 12, centrifugal 3 minutes of 000r/min rotating speed is removed supernatant; At this moment DNA promptly is adsorbed on glue pearl or the bead, glue pearl or bead is suspended in the storage liquid, and preserves standby in 4 ℃ environment.
2. the DNA that extracts is deposited in souvenir:
Take a clean container, the washing container more than once of the alcohol with 70% is no less than 3 times with the washing of DNA storage liquid again; Put into the glue pearl or the bead that are adsorbed with DNA, in container, add the DNA storage liquid then, sealing, isolated with air.
Claims (9)
1, a kind of souvenir that includes DNA, described souvenir is a full hermetic container, it is characterized in that being equipped with in the inner chamber of full hermetic container the glue pearl or the bead of adsorption of DNA.
2, a kind of souvenir that includes DNA according to claim 1, the inner chamber that it is characterized in that described full hermetic container is the transparent body.
3, a kind of souvenir that includes DNA according to claim 1 is characterized in that described full hermetic container is pendant, neonate's souvenir or forefathers' board.
4, a kind of souvenir that includes DNA according to claim 3 is characterized in that the pendant of described pendant for crystal, headwear, wrist-watch, brooch, ring, earrings or necklace ornaments.
5, the described manufacture method that includes the souvenir of DNA of a kind of claim 1 is characterized in that described manufacture method carries out according to following step: a, extract DNA according to conventional method from human body; B, in dna solution, add 0.1～0.4ml lauryl sodium sulfate and 0.2～2ml glue pearl or bead, reversing mixing 5～6 minutes, 9000～14000r/min rotating speed is centrifugal 30～60 seconds under the room temperature, removes supernatant; Add ethanol and fully mix, 9000～14000r/min rotating speed is centrifugal 30～60 seconds under the room temperature, removes supernatant, adds ethanol again and fully mixes, and 9000～14000r/min rotating speed is centrifugal 1～5 minute under the room temperature, removes supernatant; At this moment DNA is adsorbed on glue pearl or the bead, then glue pearl or bead is suspended in the DNA storage liquid, and preserves standby in 4 ℃ environment; C, the glue pearl or the bead that will be adsorbed with DNA are put into container, and sealing is isolated with air.
6, a kind of manufacture method that includes the souvenir of DNA according to claim 5 is characterized in that described DNA derives from cast-off cells in blood of human body, hair or the oral cavity.
7, a kind of manufacture method that includes the souvenir of DNA according to claim 5, the concrete operations step that it is characterized in that described c step is as follows: take a clean container, with alcohol washing container more than once, be no less than 3 times with the washing of DNA storage liquid again; Put into the glue pearl or the bead that are adsorbed with DNA, in container, add the DNA storage liquid then, sealing, isolated with air.
8,, it is characterized in that described DNA storage liquid is ethanol or organic alkene according to claim 5 or 7 described a kind of manufacture methods that include the souvenir of DNA.
9, a kind of manufacture method that includes the souvenir of DNA according to claim 5, the adsorbance that it is characterized in that DNA on described glue pearl or the bead is 1～100 μ g.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610009826 CN1817263A (en) | 2006-03-17 | 2006-03-17 | Souvenir containing DNA and production thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610009826 CN1817263A (en) | 2006-03-17 | 2006-03-17 | Souvenir containing DNA and production thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1817263A true CN1817263A (en) | 2006-08-16 |
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ID=36917619
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200610009826 Pending CN1817263A (en) | 2006-03-17 | 2006-03-17 | Souvenir containing DNA and production thereof |
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| CN (1) | CN1817263A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102511980A (en) * | 2011-12-31 | 2012-06-27 | 福建师范大学 | Preparation method for preparing mementoes storing genetic information of human bodies in direct coating manner |
| CN103009899A (en) * | 2011-09-26 | 2013-04-03 | 南京金斯瑞生物科技有限公司 | Genetic material-carried souvenir and preparation method thereof |
| CN103720143A (en) * | 2013-11-28 | 2014-04-16 | 肖义军 | Pearl containing human body cell and manufacturing method of pearl |
| AT520772A2 (en) * | 2017-12-22 | 2019-07-15 | Innotech Holding Gmbh | A method for treating a porous jewel and apparatus for preparing a liquid solution for treating a porous jewel |
| CN110074517A (en) * | 2019-04-28 | 2019-08-02 | 白城师范学院 | A kind of phytochrome and the key combined chain of genome and preparation method thereof |
| FR3096823A1 (en) * | 2019-05-28 | 2020-12-04 | Benjamin Duclos | Method of personalizing an item for identification purposes, and corresponding personalized item |
-
2006
- 2006-03-17 CN CN 200610009826 patent/CN1817263A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103009899A (en) * | 2011-09-26 | 2013-04-03 | 南京金斯瑞生物科技有限公司 | Genetic material-carried souvenir and preparation method thereof |
| CN102511980A (en) * | 2011-12-31 | 2012-06-27 | 福建师范大学 | Preparation method for preparing mementoes storing genetic information of human bodies in direct coating manner |
| CN103720143A (en) * | 2013-11-28 | 2014-04-16 | 肖义军 | Pearl containing human body cell and manufacturing method of pearl |
| AT520772A2 (en) * | 2017-12-22 | 2019-07-15 | Innotech Holding Gmbh | A method for treating a porous jewel and apparatus for preparing a liquid solution for treating a porous jewel |
| AT520772A3 (en) * | 2017-12-22 | 2020-02-15 | Innotech Holding Gmbh | Device for producing a liquid solution for treating a porous piece of jewelry |
| AT520772B1 (en) * | 2017-12-22 | 2020-02-15 | Innotech Holding Gmbh | Device for producing a liquid solution for treating a porous piece of jewelry |
| CN110074517A (en) * | 2019-04-28 | 2019-08-02 | 白城师范学院 | A kind of phytochrome and the key combined chain of genome and preparation method thereof |
| FR3096823A1 (en) * | 2019-05-28 | 2020-12-04 | Benjamin Duclos | Method of personalizing an item for identification purposes, and corresponding personalized item |
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