CN1815214A - Biological sensor for multiparameter integrated piological-chemical detection - Google Patents
Biological sensor for multiparameter integrated piological-chemical detection Download PDFInfo
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- CN1815214A CN1815214A CN 200510031238 CN200510031238A CN1815214A CN 1815214 A CN1815214 A CN 1815214A CN 200510031238 CN200510031238 CN 200510031238 CN 200510031238 A CN200510031238 A CN 200510031238A CN 1815214 A CN1815214 A CN 1815214A
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- piological
- cholesterol
- uric acid
- electrochemical reaction
- chemical detection
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Abstract
Present invention belongs to no radiation detective instrument which uses organism enzyme technology foundation, a biosensor adopting electrochemical method testing glucose, uric acid and cholesterol parametric in blood. It features adopting screen print technology printing silver, carbon syrup and insulating paint to substrate material layer, finally over printing self-adhesive to form three electrochemical reaction pools. It can combine relevant detection instrument time-sharing and stepped taking the measurements blood sugar, uric acid and cholesterol three physiological parameters, to satisfy medical clinical detective variety requirement and domestic use.
Description
Technical field
The invention belongs in the medical domain and need not radiate the instrument that detects, it is based on biological enzyme technology, the biology sensor that adopts electrochemical method that the test substance in the blood is tested specifically is the biology sensor of glucose, uric acid and cholesterol three parameters in the test blood.
Background technology
At present, the whole world has 100,012,000 diabetics, and developed countries onset diabetes rate has reached 2%-10%, domesticly should the disease population size have also surpassed 4,000 ten thousand people, accounts for about 4% of total population.Diabetes have become human the 3rd dead killer.At present onset diabetes rate every year is with 10% speed increase, but recall rate has only 20%, that is to say, has 80% patient to get diabetes but do not know, and these people overwhelming majority are early stage patients, because of not knowing that the state of an illness very easily increases the weight of.The diabetes patient wishes the blood sugar of testing oneself oneself at home very much every day, and in time adjusts dosage by the result that tests oneself.The detection major part of diabetes is to carry out analyzing and testing by blood to obtain physiological parameter and diagnose.
Uric acid (Uric acid) is the final metabolic product of purine
[105]All can cause the rising (high lithemia disease) of blood plasma uric acid concentration or reduce (low olic acid disease) the acatharsia of uric acid if purine metabolic disturbance, energetic supersession reach kidney unusually.At present medical circle is thought, testing uric acid is the best biochemical marker of gout (Gout) due to the diagnosis purine metabolic disturbance, thus in the blood uric acid be determined at significant clinically.
Disclose according to the Ministry of Public Health, the hypertensive patient of China surpasses 6,000 ten thousand, every studies show that, and increasing of cholesterol is the cardiac trigger disease, miocardial infarction, the main arch-criminal of angiocardiopathy.Hospital's tradition method for determination of cholesterol is based on colorimetric and method for saponification at present.This method was both time-consuming, and price is high again, and needed specialized equipment and professional's operation, was difficult for popularizing and personal user's use.The cholesterol test equipment be non-mainstream product at present, but because of heart disease and cardiovascular patient increase day by day, its market demand day by day enlarges, and especially is fit to the portable testing set of family's use.
Learn by above-mentioned, blood sugar, uric acid and cholesterol are three the particularly important parameters by the blood testing health, and the present used strip of various instruments both domestic and external, all be to detect single parameter or two parameters, the strip of the multiple parameters that has has just covered blood sugar, cholesterol, the strip that does not have three parameter sets to become one.Adopt the electrochemical method test paper bar of enzyme process at present, be easy to use, measure advantage highly sensitive, that the experimental result acquisition is fast, so obtained to use widely in hospital because it has.But the test strips major part of succeeding in developing at present is based on single parameter, and test result is difficult to satisfy clinical requirement.
Summary of the invention
The present invention is in order to overcome the limitation that existing test condition can only the one-parameter test, satisfy the multiple requirement of clinical medicine and the use of convenient family, and provide a kind of can be with the biology sensor of glucose, uric acid and cholesterol integration testing, it can in conjunction with the timesharing of relevant detection instrument, the substep measure blood sugar, uric acid and three physiological parameters of cholesterol.
Biology sensor of the present invention as shown in Figure 1.Adopt silk-screen printing technique that silver slurry, carbon slurry and insullac are printed onto on the same substrate material layer 1: printing silver layer 6 on the substrate material layer 1 earlier; After treating the silver layer drying, cover stamps carbon-coating 7 thereon; After treating that then carbon-coating is done, chromatography last layer insullac 8 thereon again; At last more thereon chromatography adhesive sticker 9 form three electrochemical reaction cells 2,3,4.
Substrate material layer 1 is selected various macromolecular materials for use, as Polyvinylchloride, polypropylene or polycarbonate etc.;
Be solidified with corresponding biological enzyme membrane in the reaction chamber of glucose electrochemical reaction cell 2, electrochemical reaction of uric acid pond 3, cholesterol electrochemical reaction cell 4, as being solidified with glucose oxidase film in the glucose response chamber, be solidified with the urate oxidase film in the uric acid reaction chamber, the lentochol reaction chamber is solidified with the cholesterol oxidase film;
Covering rete 10 is transparent hydrophilic material, and thickness is 0.15mm, selects polyvinyl alcohol material or other hydrophilic material for use.
Advantage of the present invention demonstrates fully:
It on same substrate material layer 1, is separated from each other glucose, uric acid and the design of cholesterol enzyme membrane, and a shared common port does not disturb during electrochemical reaction mutually; Each electrochemical reaction in the reaction tank is separate, and the constant potential timesharing applies step by step, has avoided the galvanochemistry in the test process to disturb each other; It can be measured blood sugar, uric acid and three physiological parameters of cholesterol in conjunction with the timesharing of relevant detection instrument, substep, satisfies the multiple requirement of clinical medicine detection and the use of convenient family.
Description of drawings
Fig. 1 is the one-piece construction synoptic diagram of biology sensor of the present invention.
Fig. 2 is the synoptic diagram of biology sensor conductive silver layer 6, and conductive silver layer 6 has 6, and per two is a pair of combination, transmits the electronics that produces in the electrochemical reaction jointly, for testing circuit provides effective detection electric current.
Fig. 3 is the synoptic diagram of biology sensor carbon-coating 7, and carbon-coating 7 provides corresponding reaction media for electrochemical reaction.
Fig. 4 is the synoptic diagram of biology sensor insulation course 8, and insulation course 8 provides corresponding protection for the operate as normal of test, produces to prevent that short circuit phenomenon from producing.
Fig. 5 is the synoptic diagram of biology sensor non-drying glue layer 9, and non-drying glue layer 9 is the critical pieces that form reaction chamber, pastes epiphragma 10 by it after putting biological enzyme membrane on the carbon-coating, for condition (formation siphonage) has been created in the automatic suction of blood.
Fig. 6 is the synoptic diagram of the covering rete 10 of biology sensor, and covering rete 10 is transparent hydrophilic material, and thickness is 0.15mm, selects polyvinyl alcohol material or other hydrophilic material for use.
Embodiment
Blood by suction passage 5 automatic inspirations arrives glucose electrochemical reaction cell 2, electrochemical reaction of uric acid pond 3,4 three different reaction tanks of cholesterol electrochemical reaction cell respectively, and here corresponding electrochemical reaction will take place for they; In conjunction with the timesharing of relevant detection instrument, substep blood sugar, uric acid and three physiological parameters of cholesterol are measured.
Claims (4)
1, be used for the biology sensor of multiparameter integrated piological-chemical detection, it is characterized in that:
Adopt silk-screen printing technique that silver slurry, carbon slurry and insullac are printed onto on the same substrate material layer 1: on substrate material layer (1), to print earlier silver layer (6); After treating the silver layer drying, cover stamps carbon-coating (7) thereon; After treating that then carbon-coating is done, chromatography last layer insullac (8) thereon again; At last more thereon chromatography adhesive sticker (9) form three electrochemical reaction cells (2), (3), (4).
2, the biology sensor that is used for multiparameter integrated piological-chemical detection according to claim 1 is characterized in that: substrate material layer (1) is selected various macromolecular materials for use, as Polyvinylchloride, polypropylene or polycarbonate.
3, the biology sensor that is used for multiparameter integrated piological-chemical detection according to claim 1, it is characterized in that: be solidified with corresponding biological enzyme membrane in the reaction chamber of glucose electrochemical reaction cell (2), electrochemical reaction of uric acid pond (3) cholesterol electrochemical reaction cell (4), as being solidified with glucose oxidase film in the glucose response chamber, be solidified with the urate oxidase film in the uric acid reaction chamber, the lentochol reaction chamber is solidified with the cholesterol oxidase film.
4, the biology sensor that is used for multiparameter integrated piological-chemical detection according to claim 1 is characterized in that: covering rete (10) is transparent hydrophilic material, and thickness is 0.15mm, selects polyvinyl alcohol material or other hydrophilic material for use.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510031238 CN1815214A (en) | 2005-02-02 | 2005-02-02 | Biological sensor for multiparameter integrated piological-chemical detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510031238 CN1815214A (en) | 2005-02-02 | 2005-02-02 | Biological sensor for multiparameter integrated piological-chemical detection |
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| Publication Number | Publication Date |
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| CN1815214A true CN1815214A (en) | 2006-08-09 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 200510031238 Pending CN1815214A (en) | 2005-02-02 | 2005-02-02 | Biological sensor for multiparameter integrated piological-chemical detection |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102033087A (en) * | 2009-10-02 | 2011-04-27 | 生命扫描苏格兰有限公司 | Multi-analyte test strip with inline working electrodes and shared opposing counter/reference electrode |
| CN102661984A (en) * | 2012-04-25 | 2012-09-12 | 北方工业大学 | Control circuit based on life extension of multichannel electrochemical sensor |
| DE102017208461A1 (en) | 2017-05-18 | 2018-11-22 | Diabetes.Online Ag | Multianalytmessung |
-
2005
- 2005-02-02 CN CN 200510031238 patent/CN1815214A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102033087A (en) * | 2009-10-02 | 2011-04-27 | 生命扫描苏格兰有限公司 | Multi-analyte test strip with inline working electrodes and shared opposing counter/reference electrode |
| CN102661984A (en) * | 2012-04-25 | 2012-09-12 | 北方工业大学 | Control circuit based on life extension of multichannel electrochemical sensor |
| DE102017208461A1 (en) | 2017-05-18 | 2018-11-22 | Diabetes.Online Ag | Multianalytmessung |
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