CN1814744A - Cell filter - Google Patents
Cell filter Download PDFInfo
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- CN1814744A CN1814744A CN 200510122884 CN200510122884A CN1814744A CN 1814744 A CN1814744 A CN 1814744A CN 200510122884 CN200510122884 CN 200510122884 CN 200510122884 A CN200510122884 A CN 200510122884A CN 1814744 A CN1814744 A CN 1814744A
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Abstract
This invention discloses a method for manufacturing microliquid cell filters for filtering or separating different microliquid cells in blood, which applies a finger cross arranged method for liquid channels and sets an input cavity and an ouput cavity for the liquid in the matrix, in which, an inlet is set on the input cavity and an outlet is set on the output cavity and an isolating slot is set between them and connects them, thus more interface areas are got between the input and output channels and suitable for forming more slot structures for blocking, ordinarily, said method applies the microprocess technology to manufacture components of an organelle or dies of the components, the filter can be made of compatible materials of soft spring material and biology to be implanted into the human body working for a long time.
Description
Technical field
The present invention relates to a kind of device that carries out cellular segregation, relate in particular to a kind of being used for according to the cellifugal cell filter of volume integral.
Background technology
Particulate state formed elements main in chemical analysis or the biological sample is a cell.From the biological fluid of complicated component, separate the single or micro-special cell of trace, become a kind of biomedical engineering technology that becomes more and more important.This technology can detect cancer patient be discharged in the blood cancerous tissue or the trace cancer cells, be convenient to the doctor the monitoring state of an illness development and carry out the early diagnosis of cancer.It is clinical in the body blood filtration to adopt this technology to realize, promptly directly be separated to the high reactivity sample in the stage of respectively falling ill on one's body from cancer patient, this is for research cancer pharmacology, the genetic expression of cancer cells and the research of new treatment provide a kind of new fast, not damaged, high efficiency method.The removing of the quick cancer cells in the organ transfer operation, from maternal peripheral blood liquid, carry out fetal cell extraction being used for the gene examination of fetus, or the like these fields all need rapidly and efficiently a kind ofly, the technology that Wicresoft hinders is carried out the separation of cell.
The most frequently used cell extraction technology is a flow cytometer at present, and this technology is based on the size of cell, and charge characteristic and immunological characteristic carry out the counting of cell, the classification and catch, be a kind of poor efficiency but itself have, expensive, be difficult for universal shortcoming, be not suitable in clinical use.Also have some cell separation technologies to be based on the method that the antibody technique for fixing separates and catch cell in addition, though that immune response has is very strong special
Summary of the invention
The present invention can provide a kind of high-level efficiency, easily processing, the cell filter of low integrated cost.
The present invention adopts following technical scheme:
A kind of being used for according to the cellifugal cell filter of volume integral, comprise matrix, in matrix, be provided with fluid inflow chamber and fluid exhaust chamber, on the fluid inflow chamber, be provided with fluid intake, be provided with fluid outlet at the fluid exhaust chamber, be provided with at fluid inflow chamber and fluid exhaust chamber and intercept seam and fluid inflow chamber and stitch by obstruct with the fluid exhaust chamber and be communicated with.
Compared with prior art, the utlity model has following advantage:
The present invention is that a kind of making is simple, design ingeniously, uses cell filter flexibly, is used for fluid environment, can will be similar to this tangible particulate component of biomass cells, blocks with deformability and separates according to its volume is big or small.According to the order of magnitude of cell to be separated, adopt the obstructs seam of different sizes, and the described cell filter of technique scheme is carried out multistage combination by the size order that intercepts seam, block when can realize multiple composition and separate.Because cell filter provided by the present invention is made relatively easy, manufactured materials is various, can be applied to blocking in the application of acellular class equally and go, and it is more little to block particle volume, and advantage is obvious more.Concrete advantage is as follows:
1. owing to the congenital reason in this cell filter design, input terminus and output terminal, comprise that fluid intake path and fluid outlet path will inevitably form one and be lower than the sagging fluid pool that blocks the operating water plane, this fluid can hold wherein blocking the particulate component that gets off just, reach the effect of avoiding the particulate matter plugged slots, block efficient thereby improve, improve the fluid throughput.
2. the method that making processes adopted of cell filter proposed by the invention makes and makes complex three-dimensional steric hindrance structure originally, is transformed into by two kinds of simple two-dimensional two dimensional structures encapsulation to form, and method for packing is decided according to used work material.
3. common complete processing, micro fabrication particularly, relating to two elements that figure is all arranged when superposeing, often need complicated alignment device, but cell filter proposed by the invention is when two machine component assemblings, requirement to alignment precision reduces greatly, does not in most cases need Special Equipment manually to carry out.
Make small (micron order) block structure the time because resilient material is easy to take place deformation, make microstructure that very big deformation take place.The cell filter that this patent proposed is using resilient material to process, and when assembling and application, does not have obvious external force and acts on microstructure, so the possibility of microstructure generation deformation reduces greatly.
5. when blocking the particle of different sizes and deformation characteristics,, promptly change the degree of depth and the width of formation slit groove as long as change the wherein structure of a block element.This change is relatively easy for complete processing.
6. the particle that is blocked is more little, and the advantage of this cell filter is obvious.
7. owing to can adopt soft elasticity, the macromolecular material of height bio-compatibility, cost of manufacture is lower, so this cell filter can be applied in some patient's the extraction at the somatocyte sample, the long term operation that it can be implanted of microminiaturized characteristics is as the removing of metastasis of cancer cell.
8. this cell filter is made simply, is easy to carry out batch, makes cheaply.
9. the up-stream module that can be used as micro-analysis system is carried out sample pretreatment, its cost, and efficient and integrated cost are better than general design.
Description of drawings
Fig. 1 is a D-D sectional view of the present invention.
Fig. 2 is a C-C sectional view of the present invention.
Fig. 3 is an A-A sectional view of the present invention.
Fig. 4 is a B-B sectional view of the present invention.
Fig. 5 is the flow process chart of making method embodiment of the present invention.
Fig. 6 is the flow process chart of another embodiment of making method of the present invention.
Fig. 7 is the flow process chart of an embodiment again of making method of the present invention.
Embodiment
A kind of being used for according to the cellifugal cell filter of volume integral, comprise matrix 4, in matrix 4, be provided with fluid inflow chamber 1 and fluid exhaust chamber 2, on fluid inflow chamber 1, be provided with fluid intake 11, be provided with fluid outlet 21 at fluid exhaust chamber 2, be provided with at fluid inflow chamber 1 and fluid exhaust chamber 2 and intercept seam 3 and fluid inflow chamber 1 and stitch 3 with fluid exhaust chamber 2 by obstruct and be communicated with, in the present embodiment, matrix 4 comprises base plate 41 and cover plate 42, fluid inflow chamber 1 and fluid exhaust chamber 2 are located on the base plate 41, intercept seam 3 and be located on the cover plate 42, base plate 41 planes that are provided with fluid inflow chamber 1 and fluid exhaust chamber 2 be provided with intercept stitch 3 cover plate 42 planes mutually sealing-in be connected.The present invention can single only use, also can the in parallel or use that is cascaded of a plurality of stacks.Promptly can be processed into plane mode originally and use, also can bend to other shape and use.
The present invention can adopt photoetching making, generally can adopt common soft lithographic technology, ordinary silicon wet corrosion technique, the deep technology of plasma dry method etc.Its main technique flow process comprises:
Soft lithographic technology:
1. at first according to service requirements design and the used mask of making photoetching; 2. on silicon chip that clean is in advance crossed or sheet glass, be coated with one deck thick photoresist, after technology is finished, form the masterplate of fluidic inflow and outflow passage; 3. cross in clean in advance and be coated with the thin photoresist material of last layer on silicon chip or the sheet glass, other thickness wants according to institute that overanxious cell volume is big or small decides the slit that filteration is finished in formation after technology is finished; The substrate (silicon or glass) that 4. will scribble photoresist material carries out photoetching process, comprises exposure, develop, and the heat baking, processing such as sclerosis finally obtain processing the mould of the cover plate up and down of cell filter.The assembly of the cell filter that utilizes resulting mould to cast to obtain macromolecular material obtains practical device through assembling.
Technical process is seen Appendix.
The ordinary silicon wet corrosion technique:
1. at first according to service requirements design and the used mask of making photoetching; 2. on silicon chip that clean is in advance crossed or sheet glass, plate one deck silicon nitride or other corrosion resistant film; 3. coat photoresist material being coated with on the substrate of film, utilize the mask of making in advance to expose, after the development treatment, remove the film removal that comes out, expose the silicon or the glass surface that need etching with corresponding corrosive fluid; 4. carry out etching with silicon or glass erosion liquid, finally obtain to carry out the masterplate of cell filter assembled components or parts in order to casting or impression cell filter parts.
Technical process is seen Appendix.
The deep technology of plasma dry method
Because contain darker groove in the cell filter, so generally adopt the dry etch process of ICP high aspect ratio in dry etching, main flow process comprises: 1. carry out mask design and making as requested; 2. substrate (silicon, glass, macromolecular material etc.) pre-treatment; 3. substrate surface resist coating, exposure, developing to need the part of etching to expose; 4. in ICP etching machine, carry out etching.Finally obtain assembling the masterplate of the parts of cell filter or assembling parts so that cast or impress the parts that obtain assembling cell filter.
Claims (2)
1, a kind of being used for according to the cellifugal cell filter of volume integral, it is characterized in that comprising matrix (4), in matrix (4), be provided with fluid inflow chamber (1) and fluid exhaust chamber (2), or (1) is as exhaust chamber, (2) as inflow chamber, on fluid inflow chamber (1), be provided with fluid intake (11), be provided with fluid outlet (21) at fluid exhaust chamber (2), be provided with at fluid inflow chamber (1) and fluid exhaust chamber (2) and intercept seam (3) and fluid inflow chamber (1) and stitch (3) with fluid exhaust chamber (2) by obstruct and be communicated with.
2, cell filter according to claim 1, it is characterized in that matrix (4) comprises base plate (41) and cover plate (42), fluid inflow chamber (1) and fluid exhaust chamber (2) are located on the base plate (41), intercept seam (3) and be located on the cover plate (42), base plate (41) plane that is provided with fluid inflow chamber (1) and fluid exhaust chamber (2) be provided with cover plate (42) plane that obstruct stitches (3) mutually sealing-in be connected.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510122884 CN1814744A (en) | 2005-12-06 | 2005-12-06 | Cell filter |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510122884 CN1814744A (en) | 2005-12-06 | 2005-12-06 | Cell filter |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1814744A true CN1814744A (en) | 2006-08-09 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200510122884 Pending CN1814744A (en) | 2005-12-06 | 2005-12-06 | Cell filter |
Country Status (1)
| Country | Link |
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| CN (1) | CN1814744A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104968408A (en) * | 2013-02-05 | 2015-10-07 | 普凯尔德诊断技术有限公司 | Filter arrangement and method for using the same |
-
2005
- 2005-12-06 CN CN 200510122884 patent/CN1814744A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104968408A (en) * | 2013-02-05 | 2015-10-07 | 普凯尔德诊断技术有限公司 | Filter arrangement and method for using the same |
| CN104968408B (en) * | 2013-02-05 | 2019-09-17 | 普凯尔德诊断技术有限公司 | Filter device and the method for using the filter device |
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