CN1813060A - Plant cells and plants with increased tolerance to environmental stress - Google Patents

Abstract

The invention generally relates to plant cells and plants which are transformed and a process for preparing the plant cells and the plants. The plant cells and the plants contain genes which lead the tolerance and/or resistance which are increased for the environmental stress to be inactive or down-regulated compared with wild type cells which are not transformed. The invention also generally relates to the plant cells which are transformed and are provided with changed metabolic activity compared with corresponding wild type cells which are not transformed, a method for generating, screen-selecting and fostering the plant cells and the plants and a method for checking the stress in the plant cells and the plants, wherein the genes which are inactive or down-regulated change the metabolic activity and lead the tolerance and/or resistance which are increased for the environmental stress compared with the corresponding wild type cells which are not transformed.

Description

The vegetable cell and the plant that environment-stress are had the patience of raising

The method that generality of the present invention relates to plant transformed cell and plant and produces this vegetable cell or plant, described plant or vegetable cell contain and cause comparing environment-stress with unconverted wild-type cell and improve patience and/or the inactivation of resistance or the gene of downward modulation.

The present invention is also general to be related to the method for the plant transformed cell of comparing the metabolic activity with change with corresponding unconverted wild-type plant cell and this class vegetable cell of preparation, screening and cultivation or plant and detects the method for coercing in vegetable cell or the plant, wherein pass through the gene alteration metabolic activity of inactivation or downward modulation, and the change of metabolic activity causes comparing patience and/or the resistance that environment-stress improves with corresponding unconverted wild-type plant cell.

Particularly, the method that the present invention relates to plant transformed cell and plant and produce this vegetable cell or plant, described vegetable cell or plant contain (particularly by changing metabolic activity) and cause comparing environment-stress raising patience and/or the inactivation of resistance or the gene of downward modulation with unconverted wild-type cell.

Coercing as drought stress, salt stress, heat stress and cold abiotic environment of coercing is that (Boyer.1982.Science 218,443-448) for the main limiting factor of plant-growth and productivity.Show as important economy and political factor by these crop loss and crop yield losses of coercing the staple crops that cause such as rice, Zea mays (corn) and wheat, and cause the food shortage of many under-developed countries.

Plant is exposed in its life cycle under the condition of ambient water content minimizing usually.Most of plant has been evolved out and protected the strategy of self under low water or drying (arid) condition.Yet if the intensity of drought condition is too big, the time length is oversize, its influence to development of plants, growth and most of crop yields is great.Continue to be exposed to the bigger variation that arid causes plant metabolism.Great variety in these metabolism finally causes necrocytosis and therefore causes production loss.

Develop that anti-to coerce plant be possible solve or reconcile the strategy of subproblem (McKersie and Leshem, 1994, " Stress and Stress Coping in Cultivated Plants ", KluwerAcademic Publishers) at least.Yet development is slow relatively to the traditional plant breeding strategy of new plant that coercing of these kinds shows resistance (patience), and needs the plant of specific resistance and the plant hybridization of needs.Be used for the limited germ plasm resource of stress tolerance and concern that the uncompatibility of plant species intermolecular hybrid far away is the conventional major issue that runs into of cultivating.In addition, the n cell process that causes arid, cold-peace salt patience is complicated and relates to cell adapted mechanism and multiple and a large amount of pathways metabolism (McKersie and Leshem, " 1994. Stress and Stress Coping in CultivatedPlants ", Kluwer Academic Publishers).Multicomponent characteristic of stress tolerance not only makes the patience breeding unsuccessful to a great extent, but also has limited the ability of using biotechnological means genetic design stress-tolerance plant.

Arid, heat, cold-peace salt stress have the common factor very important for plant-growth, the i.e. availability of water.Plant is exposed in its whole life under the condition of ambient water content minimizing.Most of plant has been evolved out and has protected the strategy of self under these conditions.Yet if the intensity of drought condition is too big, the time length is oversize, its influence to development of plants, growth and most of crop yields is great.Because the high content of salt in some soil causes the absorbable moisture of cell and reduces, so that its influence is similar under drought condition is viewed.In addition, when being lower than freezing temperature, begin to form ice in the apoplast and from synplasm, capture moisture and cause vegetable cell loss moisture (McKersie and Leshem, 1994, " Stress and Stress Coping in CultivatedPlants ", Kluwer Academic Publishers).Usually, plant is similar to each molecular reaction mechanism of these stress conditions.

Present result of study shows that arid patience is complicated quantitative performance, and does not obtain real discriminating mark so far.High salt concentration or dehydration may cause the infringement of cell levels under drought stress, but injure accurately and imperfectly understand (Bray, 1997.Trends Plant Sci.2,48-54).The shortage that mechanism is understood makes that being difficult to design the transgenosis approach improves arid patience.Yet an important results of damage may be the generation that causes the active oxygen radical of cell injury, the modification of described damage such as peroxidatic reaction of lipid or protein and nucleic acid.The reaction of the chemical property of oxyradical and they and cell assembly (as cytolemma) has a detailed description (McKersie and Leshem, 1994, " Stress and Stress Coping in Cultivated Plants ", Kluwer AcademicPublishers).

A lot of high degree of controlled and oxygen activation site (McKersie and the Leshem of tight coupling to avoid intermediate product to discharge are arranged in the vegetable cell, 1994.Stress and Stress Coping inCultivated Plants, Kluwer Academic Publishers).In the abiotic stress environment, this control or coupling may be destroyed, and should effect " dysfunction " can leak active oxygen.The active oxygen of various ways that if these uncoupling incident durations are short and oxygen scavenging system can detoxify, then these uncoupling incidents are harmless.If oxygen production has surpassed plant toxicide ability, deleterious reaction of degeneration then takes place.At subcellsular level, the decomposition of film and proteinic gathering are classical symptoms.Therefore oxygen production and remove between balance be critical to the positive growth of keeping plant and metabolism and overall environment (abiotic) stress tolerance and/or resistance.

The accumulation that stops or reduce the oxyradical that responds to arid be the potential approach that changes patience (Allen, 1995.Plant Physio.107,1049-1054).The expression of crossing of antioxidase or ROS scavenger enzyme is a kind of possibility of introducing functional detoxification system.For example, the transgenic alfalfa plant of expressing the Mn superoxide-dismutase after lack of water is coerced, tend to have the injury of minimizing (McKersie etc., 1996.Plant Physio.111,1177-1181).Same transgenic plant have the biomass of raising to produce (McKersie etc., 1999.Plant Physiology, 119:839-847 in field test; McKersie etc., 1996.Plant Physiol.111,1177-1181).The transgenic plant of excessive generation penetrant such as N.F,USP MANNITOL, Polylevulosan, proline(Pro) or trimethyl-glycine also show the resistance of raising to the abiotic stress of some forms, and think that the synthetic penetrant is as ROS scavenging agent play a role (1993Science 259 such as Tarczynski., 508-510; Sheveleva waits 1997.Plant Physiol.115,1211-1219).

The objective of the invention is to identify new, the unique gene that plant stress patience can be provided by inactivation or downward modulation.

Another object of the present invention is to identify, prepare and cultivate new, unique anti-coercing or/and anti-vegetable cell or plant and the method for inducing and detect stress tolerance and/or resistance in plant or vegetable cell of coercing.Another object of the present invention is to identify the novel method that detects stress tolerance and/or resistance in plant or vegetable cell.

The present invention also aims to identify inactivation or down timing new unique gene of plant stress patience can be provided, described patience preferably realizes by changing metabolic activity.

The invention provides the transformed plant cells of comparing metabolic activity with corresponding unconverted wild-type plant cell with change, wherein pass through the gene alteration metabolic activity of inactivation or downward modulation, and the change of metabolic activity causes comparing environment-stress raising patience and/or resistance with corresponding unconverted wild-type plant cell.

Term used herein " metabolite " refers to intermediate material, preferably the low-molecular-weight material that occurs in cell or phytosynthesis metabolism and katabolism.

Term " metabolic activity of change " is meant with respect to respective volume contrast, reference or the wild-type of (as in biology, tissue, cell or cellular compartment), the variation of metabolite quantity, concentration or activity in the designated volume (effective concentration that refers to chemical reaction and other mass actions) (improve or reduce), it compares and changes (improve or reduce) with corresponding unconverted wild-type plant cell, can be by a kind of measurement the in the following method for example.

Term used herein " gene of inactivation or downward modulation " is meant the metabolic activity that transgenosis reduces or disappearance changes of the expression of nucleic acid that causes sequence chart 1a, 1b, 1c or 1d, causes thus comparing patience and/or the resistance that environment-stress improves with corresponding unconverted wild-type plant cell.

In transgenic plant cells of the present invention, the reduction of described expression of nucleic acid or disappearance cause the patience that compares the environment-stress raising with corresponding unconverted wild-type plant cell, and described patience preferably realizes by changing metabolic activity.Wherein environment-stress is selected from salinity, arid, temperature, metal, pharmaceutical chemicals, cause of disease and oxidative stress or its combination, preferred arid and/or temperature.

Term " expression " is meant gene fragment or the gene transcription and/or the expression of coding meaning.Usually the product that produces is mRNA or protein.Yet expression product also can comprise functional r NA such as antisense strand, nucleic acid, tRNAs, snRNAs, rRNAs, RNAi, siRNA, ribozyme etc.Expression can be whole, partial or instantaneous, for example is confined to some cell type, tissue, organ or period.

Except as otherwise noted, term " polynucleotide ", " nucleic acid " and " nucleic acid molecule " are used interchangeably in the context of the invention.Except as otherwise noted, term " peptide ", " polypeptide " and " protein " are used interchangeably in the context of the invention.Term " sequence " can relate to polynucleotide, nucleic acid, nucleic acid molecule, peptide, polypeptide and protein, depends on the linguistic context of using term " sequence ".Term used herein " gene ", " polynucleotide ", " nucleotide sequence ", " nucleotide sequence " or " nucleic acid molecule " use the Nucleotide that is meant random length polymer form herein, comprise Yeast Nucleic Acid or thymus nucleic acid.Term only relates to the primary structure of molecule.

Therefore, term used herein " gene ", " polynucleotide ", " nucleotide sequence ", " nucleotide sequence " or " nucleic acid molecule " comprise double-stranded and single stranded DNA and RNA.The modification that it also comprises known type, for example methylate, " adding cap ", substitute one or more naturally occurring Nucleotide with analogue.Preferred DNA of the present invention or RNA sequence contain the encoding sequence that coding this paper defines polypeptide.

" encoding sequence " is meant when placing the suitable adjustable sequence to control the nucleotide sequence that following time is transcribed into mRNA and/or is translated as polypeptide.The border of encoding sequence is by 5 ' terminal translation initiation codon and 3 ' terminal translation stop codon decision.Encoding sequence can include, but is not limited to mRNA, cDNA, recombinant nucleotide sequence or genomic dna, and wherein intron had better be present in certain environment.

Term " minimizing ", " reduction " or " disappearance " relate to the corresponding change of character in biological, biological part (as tissue, seed, root, leaf, flower etc.) or cell." change of character " is interpreted as proteinic contrast, reference or the wild-type with respect to respective volume or quantity, activity, expression level or the quantity of gene product or the variation of metabolite content in the designated volume or in the specific quantity protein.Preferably when minimizing, reduction or disappearance relate to the active minimizing of gene product, reduction or disappearance, overall activity reduces, reduces or lacks in the volume, no matter whether the specific activity of the quantity of gene product or gene product or both reduce, reduce or lack, or whether the gene of the quantity of nucleotide sequence, stability or translation efficiency or encoding gene product reduces, reduces or lack.

Term " minimizing ", " reduction " or " disappearance " comprise that described character only changes to some extent in the part of research object of the present invention, for example modify and be found in cellular compartment (as organoid), or in the plant part (as tissue, seed, root, leaf, flower etc.), if but detect whole research object (being complete cell or plant), then detect less than.Preferably " minimizing ", " reduction " or " disappearance " see cell, so term " active minimizing, reduction or disappearance " or " minimizing of metabolite content, reduction or disappearance " relate to wild-type cell and compare the minimizing in the cell, reduction or disappearance.In addition, term " minimizing ", " reduction " or " disappearance " comprise that described characteristic only uses variation in the biological different growing stages in the inventive method, for example reduce, reduce or only lack at seed growth or generation when blooming.In addition, term for example comprise since the RNAi that uses not stable integration in the biological gene group and therefore have only transcribing minimizings, reduce or lacking of temporal effect.

Therefore, term " minimizing ", " reduction " or " disappearance " are meant enzyme or other protein or regulate the specific activity of RNA and the quantity of compound or metabolite (polypeptide for example of the present invention, nucleic acid molecule or high-purity chemical thing or coding RNA or DNA) reduces, reduces or lacks in unit volumes.

Term " wild-type ", " contrast " or " reference " are used interchangeably and can are cell or a biological part (as organoid or tissue) or biology, the especially microorganism or the plant of modifying or handling without the method according to this invention.Correspondingly, will meet as much as possible with cell, biology or its are local as the cell of wild-type, contrast or reference or a biological part (as organoid or tissue) or biology (especially microorganism or plant), and any other character except that the inventive method result is consistent as far as possible with research object of the present invention.Therefore, it is consistent or consistent as far as possible that the processing of wild-type, contrast or reference is wanted, and promptly has only the conditioned disjunction character of the quality that does not influence test character just can be different.

Preferably, anyly relatively under conditions of similarity, carry out.Term " conditions of similarity " is meant all conditions between the experiment that will compare, for example cultivates or growth conditions, and experiment condition (for example damping fluid composition, temperature, substrate, pathogenic strains, concentration etc.) is consistent.

" reference ", " contrast " or " wild-type " are preferred not to be modified or handles and other any character object similar as far as possible to research object of the present invention according to the inventive method described herein, as organoid, cell, tissue, biology, especially plant or microorganism.With reference to, contrast or wild-type at genome, to transcribe group, protein group or metabolism prescription face similar as far as possible to research object of the present invention.Preferably, term " reference " " contrast " or " wild-type " organoid, cell, tissue or biological (especially plant or microorganism) relate to and organoid of the present invention, cell, tissue or biological (especially plant or microorganism) or a biological part almost consistent organoid in heredity, cell, tissue or biological (especially plant or microorganism), preferred 95%, more preferably 98%, even more preferably 99.00%, preferred especially especially 99.10%, 99.30%, 99.50%, 99.70%, 99.90%, 99.99%, 99.999% or higher identity.Most preferably except the gene product of giving reaction or active nucleic acid molecule or its coding or is introduced the method according to this invention correction, operation, exchange, consistent object in " reference ", " contrast " or " wild-type " are with the inventive method is used biology, cell or the organoid heredity, as organoid, cell, tissue or biology, unless gene product the method according to this invention of nucleic acid molecule and their codings changes to some extent.

Preferably, only having any different with object of the present invention aspect the cytoactive of the specific activity by for example nucleic acid molecule level of the present invention minimizing, reduction or disappearance or polypeptide of the present invention reduces, reduces or disappearance causes polypeptide of the present invention or RNA with reference to, contrast or wild-type, for example by or on the expression level of protein or RNA or activity, be meant its biological activity and/or its biochemistry or genetic cause.

Term " expression " is meant that genetic transcription is that structure RNA (rRNA, tRNA, miRNA) or messenger RNA(mRNA) (mRNA) and the latter are translated as protein subsequently.Experimentally can be by for example Northern, qRT PCR, transcribe and join together reaction (transcriptional run-on assays) or Western trace and other immunization experiments and detect and express.Result as expression decreased, reduction or disappearance is meant the result who reduces, reduces or lack as genetic transcription, relevant phenotypic characteristic occurs, for example the stress tolerance of enhanced or raising.

Therefore, preferred references object is the origin object of the inventive method.Preferably stdn for example and standardization total RNA, DNA or protein active or with reference to the expression amount of gene (as house-keeping genes such as ubiquitin, Actin muscle or ribosomal proteins) after relatively reference and research object of the present invention.

The modification of polypeptide of the present invention can directly or indirectly influence stress tolerance by a series of mechanism.For example, can reduce, reduce or lack the molecular amounts of polypeptide of the present invention or the expression quantity of specific activity or nucleic acid molecule of the present invention.Yet, also can be for example adjusting by modifying factor, or the stability that reduces or reduce the gene product of mRNA or nucleic acid molecule encoding of the present invention reduces, reduces or lacks naturally occurring expression of gene in biology.

This is applicable to minimizing, reduction or the disappearance of the combination that the expression of nucleic acid molecule of the present invention or its gene product and other activity enzyme of stress tolerance (as give) are handled equally similarly.

According to raising of the present invention, reduce or regulate composing type, for example because stable permanent transgene expression, or because the stable sudden change of the corresponding native gene of code book invention nucleic acid molecule, or because regulation and control of expressing or give the regulation and control of the gene behavior of expression of polypeptides of the present invention, also can be or instantaneous, for example because instantaneous conversion or for example transforming the double-stranded RNA nucleic acid molecule of the present invention that has under the inducible promoter control, antisense nucleic acid molecule, the induction type construct of ribozyme etc. also adds the instantaneous activation promotor in inductor (as tsiklomitsin or as mentioned below) back, or of short duration adding conditioning agent (as antagonist or inductor).

Compare with contrast, reference or wild-type, the minimizing of active amt, reduction or disappearance are preferably at least 10%, and preferably at least 30% or at least 60%, especially preferably at least 70%, 80%, 85%, 90% or more, especially especially preferably at least 95%, more preferably at least 99% or more.Most preferably active amt reduces, reduces or lacks 100%.

In this article, inactivation is meant that the enzyme of coded polypeptide or biological activity no longer can detect in biology or cell (for example plant or vegetable cell).Downward modulation for the present invention, (=reduce) be meant with the biological activity that is untreated and compare that the enzyme of coded polypeptide or biological activity are by part or minimizing substantially fully.This can realize by different cytobiology mechanism.In this article, activity can be in whole biology or (under the multicellular organism situation) in the particular of biology, reduce, with regard to plant, for example in the tissue of seed, leaf, this class of root or in other parts downward modulations.In this paper context, enzymic activity or biological activity are compared with contrast, reference or wild-type and are reduced by at least 10%, advantageously at least 20%, preferably at least 30%, especially preferably at least 40%, 50% or 60%, extremely preferably at least 70%, 80%, 85% or 90% or more, extremely preferably at least 95%, more preferably at least 99% or more.Most preferably active amt reduces, reduces or lacks 100%.

The present invention includes the multiple strategy of quantity (≈ expression), activity or the function of the protein that reduces nucleic acid encoding of the present invention or nucleotide sequence itself.The technician will appreciate that the method that can obtain a series of different proteinic quantity of influence, activity or functions in the mode of needs.

Term " biological activity " is meant the proteinic biological function of the present invention.Different with term " biological activity ", term " activity " is meant the raising of the compound output that produces by the inventive method.Term " biological activity " preferably refers at biology, tissue, polypeptide or proteinic enzyme function in cell or the cellular compartment, the transport vehicle function, the DNA packaging function, the heat shock protein(HSP) function, the recombinant protein function, the beta-galactosidase enzymes function, serine/threonine protein kitase CTR1 function, the lipase function, the enoyl CoA hydrolytic enzyme functional, UDP-glucose glucanotransferase function, the cell division protein function, flavonol synthase function, triacylglycerol lipases, MADS box protein matter function, the Rohapect MPE function, the pectin methyl esterase function, calcium transportation ATP enzyme function, protein kinase function, the lysophospholipase function, the conjugated protein function of Chlorophyll A-B, Ca ²⁺Transhipment ATP enzyme sample protein function, peroxidase function, disease-resistant RPP5 sample protein function or regulatory function.Suitable substrate is low-molecular weight compound and proteinic protein interaction mating partner." minimizing " of term biological function is meant for example under other the same terms (for example culture condition, plant age etc.), compare with the wild-type of handling without this method of the same race that belongs to together, (for example by one of following method of this paper) quantitatively reduces binding ability or the bonding strength of protein at least a substrate in biology, tissue, cell or cellular compartment.Reduce the modification that also can be regarded as substrate specificity, described substrate specificity can be by for example k _Cat/ K _mValue is represented.In this article, reduce to be at least 10%, favourable to be at least 20%, preferably at least 30%, especially preferably at least 40%, 50% or 60% than favourable function with the biophase that is untreated, extremely preferably at least 70%, 80%, 90% or 95%.Particularly advantageous embodiment is the inactivation of function.The binding partners of method (for example the passing through yeast two-hybrid system) identification of protein that can be familiar with by the technician.

Modifying (promptly reducing) can be caused by endogenous or extrinsic factor.For example, can cause active reduction in biology or its part by in plant culture, nutrition, plant soil or plant self, adding compound (as antagonist).

Under other uniform condition (for example culture condition, plant age etc.) relatively plant transformed cell and belonging to accordingly belong to together and unconverted wild-type of the same race.In the context of this article, have at least 10% with unconverted biophase comparison metabolic activity, advantageously at least 20%, preferably at least 30%, particularly at least 40%, 50% or 60%, especially especially preferred at least 70%, 80%, 90%, 95% or even 100% or more the variation be favourable.

Preferably, the variation of the metabolite concentration of transformed plant cells is the variation of comparing with corresponding unconverted wild-type.Preferably, the variation of metabolite concentration is measured via HPLC, and calculates by peak height or the peak area that target peak area in separately removes every kind of analyte (metabolite).Use single sample newly heavily to come standardized data.The value of gained produces so-called ratio divided by the mean value of analyzing gained under collating condition in the same sequence of the wild-type of growing, and it has represented the value that does not rely on analytical sequence.These ratios have shown the metabolite concentration behavior of comparing the conversion plant with the concentration in the wild-type control plant.

According to this method, the variation of comparing at least a meta-bolites concentration in the transformed plant cells with corresponding unconverted wild-type is at least 10%, advantageously at least 20%, preferably at least 40%, 60% or 80%, especially preferably at least 90%, 100% or 200%, extremely preferably at least 300%, 350%, 400%, 500%, 600%, 800%, 1000% or more.

Can be by all the statistical method determination data significancees known to those skilled in the art, preferably by the t-check, more preferably student t-checks.

In the preferred embodiment of the invention, the metabolite activity of change also refers to compare with corresponding unconverted wild-type plant cell, has increased the generation of described metabolite after metabolite that does not produce after the conversion or only producing after the conversion or conversion.

After more preferably calculating according to aforesaid method, compare with corresponding unconverted wild-type plant cell, the concentration of at least a metabolite reduces, and most preferably the concentration of at least a metabolite is zero, or the concentration of at least a metabolite improves.

The preferred metabolin of the present invention is 2; 3-dimethyl-5-phytyl chinol or 2-hydroxy-palmitic acid or 3; 4-dihydroxyphenylalanine (=DOPA) or 3-hydroxy-palmitic acid or 5-OxoPro or alanine or alpha-linolenic acid (c18:3 (c9; C12; Or alpha-tocopherol or aminoadipic acid or AHG or arginine or asparagus fern amino acid or β-apo-8'-carotenal or beta carotene or β-sitosterol or betatocopherol or (Δ-7-cis c15)); 10-）-Δ-15-- （c26:0） （20:1）γ-γ--3- （c24:0） （C18:2 （c9,c12）） （c16:0） （sinapine acid） （c18:0）udp-。

Can also change the metabolic activity relevant with one or more derivatives of one or more above-mentioned metabolites.

The preferred change and one or more relevant metabolic activities that are selected from above-mentioned metabolite.

In addition, can change and be selected from seminose, inositol, phosphoric acid salt, Aspartic Acid, Isoleucine, leucine, γ-An Jidingsuan, Glycerose (glycerinaldehyd), sucrose, Brassicasterin, Xie Ansuan, 5,8-dimethyl tocol, ubiquinone, palmitinic acid (c16:0), 2-hydroxy-palmitic acid or 2,3-dimethyl-5-phytyl chinol, β-Hu Luobusu, alpha-linolenic acid (c18:3 (c9, c12, c15)), the relevant metabolic activity of one or more metabolites in the Lyeopene.

In addition, can change and be selected from methyl furan galactoside, β-sitosterol, Δ-15-cis-nervonic acid (c24:1me), margaric acid (c17:0me), stearic acid (c18:0), methyl galactopyranoside, Gamma-Tocopherol, the linolic acid (metabolic activity that one or more metabolites in C18:2 (c9, c12)), hiragonic acid (c16:3me), shikimin, raffinose, L-glutamic acid, glutamine, udp-diphosphate glucose, proline(Pro), Threonine, isopentenylpyrophosphate, 5-oxyproline, forulic acid, the sinapinic acid are relevant.

In addition, can change and be selected from tryptophane, citrulline, Serine, L-Ala, R-Glyceric acid, arginine, 3-hydroxy-palmitic acid, putrescine, 3,4-dihydroxyphenylanaline (=DOPA), alpha-tocopherol, aminoadipic acid, anhydroglucose, β-A Piaohuluobusuquan, Δ-7-cis, 10-cis-hexadecadienoic acid (c16:2 acetone), cerinic acid (c26:0), cryptoxanthin, eicosenoic acid (20:1), the metabolic activity that one or more metabolites in fructose, the fumaric acid are relevant.

In addition, can change be selected from semi-lactosi, glyconic acid, glucose, glycerine, glycerol-3-phosphate, glycine, homoserine, isomaltose, lignoceric acid (c24:0), luteine, oxysuccinic acid, triacontanoic acid, methionine(Met), phenylalanine, pyruvic acid, ribonic acid, succsinic acid, tyrosine, zeaxanthin in the relevant metabolic activity of one or more metabolites.

In the present invention, inactivation or the downward modulation metabolic activity that cause with corresponding unconverted wild-type plant cell compare change of gene in vegetable cell.A kind of preferred wild-type plant cell is unconverted Arabidopis thaliana (Arabidopsis) vegetable cell.This sentences Arabidopis thaliana wild-type C24 is example (Nottingham Arabidopsis Stock Centre, UK; NASC Stock N906).

Other preferred wild-type plant cells are for being selected from corn, wheat, rye, oat, triticale, rice, barley, soybean, peanut, cotton, Semen Brassicae campestris, rice, barley, soybean, peanut, cotton, rape, rape, cassava, pepper, Sunflower Receptacle, flax, the Borrago officinalis, safflower, Semen Lini, Flower of Beltleaf Primrose, Semen Brassicae campestris, turnip rape, Flower of Aztec Marigold, plant of Solanaceae, potato, tobacco, eggplant, tomato, broad bean, pea, clover, coffee, cocoa, tea, Salix, oil palm, coconut, the unconverted vegetable cell of perennial herb and fodder crop etc.

Preferred wild-type plant cell is unconverted linum cell, preferred flax (Linum usitatissimum), more preferably kind is Brigitta, Golda, Gold Merchant, Helle, Juliel, Olpina, Livia, Marlin, Maedgold, Sporpion, Serenade, Linus, Taunus, Lifax or Liviola, unconverted Helianthus (Helianthus) vegetable cell, preferred Sunflower Receptacle (Helianthus annuus), preferred kind is Aurasol, Capela, Flavia, Flores, Jazzy, Palulo, Pegasol, PIR64A54, Rigasol, Sariuca, Sideral, Sunny, Alenka, Candisol or Floyd, or unconverted brassica plant cell, preferred colea (Brassica napus), more preferably kind is Dorothy, Evita, Heros, Hyola, Kimbar, Lambada, Licolly, Liconira, Licosmos, Lisonne, Mistral, Passat, Serator, Siapula, Sponsor, Star, Caviar, Hybrido, Baical, Olga, Lara, Doublol, Karola, Falcon, Spirit, Olymp, Zeus, Libero, Kyola, Licord, Lion, Liraj et, Lisbeth, Magnum, Maja, Mendel, Mica, Mohican, Olpop, Ontarion, Panthar, Prinoe, Pronio, Susanna, Talani, Titan, Transfer, Wiking, Woltan, Zeniah, Artus, Contact or Smart.

Change with the metabolic activity that obtains to cause environment-stress being improved patience and/or resistance owing to needn't introduce new gene, so the inactivation of gene is favourable with downward modulation.Only stoped the expression of native gene.

The inactivation of gene or reduce the direct or indirect stress tolerance that influences plant preferably influences the metabolic activity of transformed plant cells.Preferably they influence the activity of above-mentioned metabolite.

The change of preferred metabolic activity and the stress tolerance that obtains can obtain by one or more genes of the following nucleic acid sequence encoding of inactivation or downward modulation:

A) nucleic acid molecule of one of polypeptide shown in encoding sequence Fig. 1 a, 1b, 1c or the 1d;

B) contain the nucleic acid molecule of nucleic acid molecule shown at least a sequence chart 1a, 1b, 1c or the 1d;

C) nucleic acid molecule, its contained nucleotide sequence can derive from the described peptide sequence of sequence chart 1a, 1b, 1c or 1d owing to the genetic code degeneracy;

D) coding with (a) at least 50% identity is arranged and has the nucleic acid molecule of the bioactive polypeptide that sequence chart 1a, 1b, 1c or 1d protein showed to the amino acid sequence of polypeptide of the nucleic acid molecule encoding of (c);

E) coding separates and has the nucleic acid molecule of the bioactive polypeptide that sequence chart 1a, 1b, 1c or 1d protein showed to the monoclonal antibody of the polypeptide of a kind of nucleic acid molecule encoding of (d) by (a);

F) can be by the nucleic acid molecule that under stringent hybridization condition, obtains with probe or the suitable nucleic acid library of probe fragment screening, and the polypeptide of this nucleic acid molecule encoding has its minimizing or disappearance can cause the patience of environment-stress raising and/or the biological activity that protein showed of resistance, wherein said probe contains (a) sequence to one of (b) nucleic acid molecule, or described probe fragment has (a) 15nt at least to (c) institute characterisation of nucleic acids molecule, preferred 20nt, 30nt, 50nt, 100nt, 200nt or 500nt

Perhaps described nucleic acid contains and above-mentioned nucleic acid molecule complementary sequence.

According to the present invention, can particularly identify gene in the crop plants the target plant, then inactivation or reduce corresponding gene and obtain patience and/or resistance to the environment-stress raising with (preferably metabolic activity) by changing by the nucleic acid sequence encoding that is selected from sequence chart 1a, 1b, 1c or 1d sequence or its homologue.Therefore the present invention is not limited only to specified plant.

Also may compare metabolic activity that vegetable cell changes with non-stress conditions and detect environment-stress in vegetable cell or the plant by screening.This permission even when not having visible symptom, coerce level in the monitoring plant.Therefore can more early carry out counter-measure, for example the loss of crop be reduced to minimum by watering timely.

The present invention also comprises the vegetable cell of comparing the metabolic activity with change by screening under stress conditions with non-stress conditions, thereby screening has the vegetable cell or the plant of the patience and/or the resistance of raising to environment-stress.This allows not select the plant that environment-stress is had the patience and/or the resistance of raising by gene identification or visible symptom.

Compare with non-stress conditions to have the vegetable cell that changes metabolic activity and to select by screening under stress conditions and have the patience that environment is improved and/or the vegetable cell of resistance, also may cultivate according to the present invention and have the patience that environment is improved and/or the vegetable cell or the plant of resistance.Screening metabolic activity ratio such as screening-gene are faster and easier.

Screening is well-known to those skilled in the art, and generally refers to searching specific properties or proterties.This proterties of plant or vegetable cell is preferably metabolite concentration in the present invention, the concentration of preferred especially above-mentioned metabolite.Method for screening and equipment are that those skilled in the art are familiar with, and comprise GC (gas chromatography), LC (liquid chromatography (LC)), HPLC (efficient (pressure) liquid chromatography (LC)), MS (mass spectrum), NMR (nucleus magnetic resonance) spectrum, IR (infrared) spectrum, photometer method etc., and the combination of these methods.

Cultivating also is those skilled in the art's general knowledge.Be interpreted as directed plant or the vegetable cell of also stably being integrated into of specific properties or proterties.

Different incubation step is characterized with the artificial interference of clearly definition, and described interference is, instructs the pollination of parent plant system or selects suitable progeny plant as the strain of selecting to be used to hybridize.Use different cultural operations by required character decision.All technology all are well known to those skilled in the art, include, but is not limited to for example hybridize, inbreeding, back cross breeding, polyphyly breeding, multiple mixed friendship (variety blend), species hybridization, aneuploid technology etc.Hybridization technique also comprises by machinery, chemistry or biological chemistry means makes plant produce male or female sterile plants, and causes plant sterile.Male sterile plants and not homophyletic be that allogamy has been guaranteed male sterile and female Plant Genome of educating will as one man obtain the characteristic that parent plant is both sides between pollen.Can therefore be used for the improved plant strain of breeding system according to transgenic seed of the present invention and plant, the effect that it has improved ordinary method (as weedicide or pesticide treatments) maybe can make plant owing to the hereditary property of its modification is exempted method in described processing.In addition, can obtain the having improved stress tolerance novel crop of (preferred arid and temperature tolerance), described crop is because the heredity " equipment " of optimization produces the results product of comparing better quality with the product that can not tolerate corresponding unfavorable developmental condition.

But environment-stress salt provided by the invention, arid, temperature, metal, pharmaceutical chemicals, cause of disease and oxidative stress, or their combination, preferred arid and/or temperature.

Term used herein " environment-stress " refers to any inferior optimum growing condition, includes, but is not limited to and salinity, arid, temperature, metal, chemistry, cause of disease and oxidative stress or the relevant inferior optimum condition of its combination.In preferred embodiments, environment-stress can be salinity, arid, heat or low temperature or its combination, particularly low water content or low temperature.Wherein drought stress refers to any environment-stress that causes plant hydropenia or reduce the vegetation water supply, and wherein low temperature stress refers to freezing plant below 4 ℃ and freezing plant below 15 ℃, and wherein high temperature stress refers to for example be higher than 35 ℃ temperature.The scope of coercing with coerce reaction and depend on the different plants that the present invention uses, promptly as distinct between the plant of wheat and the plant as Arabidopis thaliana.Plant is the reduction of output and quality for the common reaction of environment-stress.Simultaneously also should understand specification sheets.

The present invention also provides and contains and one or more sequence chart 1a, 1b, the transformed plant cells of one or more nucleotide sequences of the sequence homology of 1c or 1d, wherein plant is selected from corn, wheat, rye, oat, triticale, rice, barley, soybean, peanut, cotton, Semen Brassicae campestris, rape, cassava, pepper, Sunflower Receptacle, flax, the Borrago officinalis, safflower, Semen Lini, Flower of Beltleaf Primrose, Semen Brassicae campestris, turnip rape, Flower of Aztec Marigold, plant of Solanaceae, potato, tobacco, eggplant, tomato, the broad bean kind, pea, clover, coffee, cocoa, tea, Salix, oil palm, coconut, perennial herb, fodder crop and Arabidopis thaliana.

The present invention also provides the transgenic plant cells of the gene of the sequence that is selected from sequence chart 1a, 1b, 1c or 1d that contains inactivation or downward modulation and/or its homologue, preferred colea, soybean (Glycinemax) or rice (Oryza sativa).

In addition can also be in the target plant, particularly identify gene in the crop plants, follow inactivation or reduce patience and/or the resistance of corresponding gene to obtain environment-stress is improved by the nucleic acid sequence encoding of the sequence that is selected from sequence chart 1a, 1b, 1c or 1d and/or its homologue.Therefore the present invention is not limited only to specified plant.

The present invention also provides and contains and sequence chart 1a, 1b, the transformed plant cells of one of 1c or 1d sequence homologous nucleotide sequence, wherein plant is selected from corn, wheat, rye, oat, triticale, paddy rice, barley, soybean, peanut, cotton, Semen Brassicae campestris, rape, cassava, pepper, Sunflower Receptacle, flax, the Borrago officinalis, safflower, Semen Lini, Flower of Beltleaf Primrose, Semen Brassicae campestris, turnip rape, Flower of Aztec Marigold, plant of Solanaceae, potato, tobacco, eggplant, tomato, the broad bean kind, pea, clover, coffee, cocoa, tea, Salix, oil palm, coconut, perennial herb, fodder crop and Arabidopis thaliana.

The present invention also provides the plant transformed cell, and its amplifying nucleic acid and sequence chart 1a, 1b, 1c or 1d sequence have at least about 30%, particularly at least about 50% homology.

According to the present invention, the plant transformed cell can be from unifacial leaf or dicotyledons.

Unifacial leaf or dicotyledons can be selected from corn, wheat, rye, oat, triticale, paddy rice, barley, soybean, peanut, cotton, Semen Brassicae campestris, rape, cassava, pepper, Sunflower Receptacle, flax, the Borrago officinalis, safflower, Semen Lini, Flower of Beltleaf Primrose, Semen Brassicae campestris, turnip rape, Flower of Aztec Marigold, plant of Solanaceae, potato, tobacco, eggplant, tomato, the broad bean kind, pea, clover, coffee, cocoa, tea, Salix, oil palm, coconut, perennial herb, fodder crop and Arabidopis thaliana.

The plant transformed cell can and can be preferably selected from dragon spruce, pine and fir from gymnosperm.

The present invention also provides the conversion plant from described vegetable cell, and described plant is unifacial leaf or dicotyledons.

Plant transformed can be selected from corn, wheat, rye, oat, triticale, paddy rice, barley, soybean, peanut, cotton, Semen Brassicae campestris, rape, cassava, pepper, Sunflower Receptacle, flax, Borrago officinalis, safflower, Semen Lini, Flower of Beltleaf Primrose, Semen Brassicae campestris, turnip rape, Flower of Aztec Marigold, plant of Solanaceae, potato, tobacco, eggplant, tomato, broad bean kind, pea, clover, coffee, cocoa, tea, Salix, oil palm, coconut, perennial herb, fodder crop and Arabidopis thaliana.

Generation is a gymnosperm from the conversion plant optimization of described vegetable cell, more preferably is selected from the plant of dragon spruce, pine and fir.

The present invention not only relate to plant and also relate to the agricultural-food that produced by arbitrary described plant transformed, plant part (as leaf, petal, flower pesticide, root, piece root, stem, bud, flower) or particularly as described in transform the seed that plant produces, described seed can be given after for inactivation or downward modulation and compare the gene of the patience that environment-stress improves or its homologue with wild-type plant is hereditary heterozygosis at least, is preferably to isozygoty.

The homologue of above-mentioned sequence can advantageously separate in yeast, fungi, virus, algae, bacterium and separate, for example from Acetobacter (subgen.Acetobacter) aceti; Have a liking for the ferrous thiobacillus of acid oxidase (Acidithiobacillus ferrooxidans); Acinetobacter (Acinetobacter sp.); Actinobacillus (Actinobacillus sp.); Aeromonas salmonicida (Aeromonas salmonicida); Agrobacterium tumefaciems (Agrobacterium tumefaciens); Super thermoduric bacteria (Aquifex aeolicus); Arcanobacterium (Arcanobacterium pyogenes); China aster aurea bacterium substance (Aster yellows phytoplasma); Bacillus (Bacillus sp.); Genus bifidobacterium (Bifidobacterium sp.); Borrelia burgdorferi (Borreliaburgdorferi); Extension brevibacterium (Brevibacterium linens); Brucella melitensis (Brucella melitensis); Buchnera sp.; Butyrivibrio fibrisolvens (Butyrivibrio fibrisolvens); Campylobacter jejuni (Campylobacterjejuni); Crescent handle bacillus (Caulobacter crescentus); Chlamydiaceae (Chlamydia sp.); Have a liking for the clothing body and belong to (Chlamydophila sp.); Mud is given birth to chlorine bacterium (Chlorobium limicola); Citric acid bacillus (Citrobacter) rodentium; Genus clostridium (Clostridium sp.); Comamonas testosteroni (Comamonastestosteroni); Corynebacterium (Corynebacterium sp.); Rickettsia burneti (Coxiella burnetii); The abnormal cocci of anti-radiation the (Deinococcusradiodurans); Plethora artiodactyl shape bacterium (Dichelobacter nodosus); Catfish tarda (Edwardsiella ictaluri); Enterobacter (Enterobacter sp.); Erysipelothrix rhusiopathiae (Erysipelothrix rhusiopathiae); Intestinal bacteria (Escherichia coli); Flavobacterium (Flavobacterium sp.); Soil draws hot Frances Salmonella (Francisella tularensis); Frankia sp.Cpl 1; Fusobacterium nucleatum (Fusobacterium nucleatum); Thermophilic bacteria (Geobacillusstearothermophilus); Gluconobacter oxydans (Gluconobacter oxydans); Hemophilus (Haemophilus sp.); Hp (Helicobacterpylori); Pneumobacillus (Klebsiella pneumoniae); Lactobacillus genus (Lactobacillus sp.); Lactococcus lactis (Lactococcus lactis); Listeria (Listeria sp.); Mannheimia haemolytica (Mannheimia haemolytic); Mesorhizobium loti; Methyl bacterium (Methylophaga thalassic) is bitten at the deep-sea; Verdigris micro-capsule cyanobacteria (Microcystis aeruginosa); The micro-capsule cyanobacteria belongs to (Microscilla sp.) PRE1; Moraxella (Moraxella sp.) TA144; Mycobacterium (Mycobacterium sp.); Mycoplasma (Mycoplasma sp.); Eisseria (Neisseria sp.); Nitrosomonas (Nitrosomonas sp.); Nostoc (Nostoc sp.) PCC 7120; Novosphingobiumaromaticivorans; Oenococcus Oeni (Oenococcus oeni); The general bacterium of lemon (Pantoeacitrea); Pasteurella multocida (Pasteurella multocida); Pediococcus pentosaceus (Pediococcus pentosaceus); Hole shape seat cyanobacteria (Phormidiumfoveolarum); Pytoplasma belongs to (Phytoplasma sp.); Bao Shi knitline algae (Plectonemaboryanum); The cud Prey of dwelling is irrigated Salmonella (Prevotella ruminicola); Propionibacterium (Propionibacterium sp.); Proteus vulgaris (Proteus vulgaris); Rhodopseudomonas (Pseudomonas sp.); Ralstonia sp.; Rhizobium (Rhizobiumsp.); Rhodococcus equi (Rhodococcus equi); Rhodothermus marinus; Rickettsia kind (Rickettsia sp.); Silent Salmonella (Riemerellaanatipestifer) in the pest of duck; Ruminococcus flavefaciens (Ruminococcus flavefaciens); Salmonella (Salmonella sp.); Selenomonas ruminantium (Selenomonasruminantium); Serratia entomophila (Serratia entomophila); Shigella (Shigella sp.); Rhizobium melioti (Sinorhizobium meliloti); Staphylococcus (Staphylococcus sp.); Streptococcus (Streptococcus sp.); Streptomyces (Streptomyces sp.); Poly-ball cyanobacteria belongs to (Synechococcus sp.); (Synechocystis sp) .PCC 6803; Thermotoga maritima (Thermotogamaritime); Treponema kind (Treponema sp.); Ureaplasma urealyticum (Ureaplasma urealyticum); Vibrio cholerae (Vibrio cholera); Vibrio parahemolyticus (Vibrio parahaemolyticus); Xyllela fastidiosa (Xylellafastidiosa); Yersinia's genus (Yersinia sp.); Zymomonas mobilis (Zymomonas mobils), preferred salmonella or intestinal bacteria or plant, preferably from yeast as from Pichia (Pichia), mycocandida (Candida), Chinese Sen Shi yeast belong (Hansenula), yeast (Saccharomyces) or plant that Torulopsis (Torulopsis) or Schizosaccharomyces (Schizosaccharomyces) etc. belong to, Arabidopis thaliana for example, corn, wheat, rye, oat, triticale, rice, barley, soybean, peanut, cotton, the Borrago officinalis, safflower, Semen Lini, Flower of Beltleaf Primrose, Semen Brassicae campestris, turnip rape, cassava, pepper, Sunflower Receptacle, Flower of Aztec Marigold, plant of Solanaceae (potato for example, tobacco, eggplant and tomato), broad bean, pea, clover, shrub plant (coffee for example, cocoa, tea), Salix, tree (oil palm for example, coconut), perennial herb (for example rye grass and fescue) and fodder crop (for example clover and trifolium), and from for example dragon spruce, pine tree or fir.More preferably can be from yeast saccharomyces cerevisiae, intestinal bacteria or plant the homologue of isolating above-mentioned sequence, preferred colea, soybean or rice.

" homologue " is defined as two nucleic acid or the protein that has similar or " homologous " Nucleotide or aminoacid sequence respectively in this article.Homologue comprises hereinafter allelic variant, lineal homologue, collateral line homologue, agonist or the antagonist of the SRP of definition.Term " homologue " comprises that in addition the degeneracy owing to genetic code is different from one of the nucleotide sequence shown in sequence chart 1a, 1b, 1c or the 1d sequence (and part), and thereby coding and sequence chart 1a, 1b, 1c or 1d sequence shown in the nucleic acid molecule of nucleotide sequence coded same SRP." naturally occurring " used herein SRP refers to the SRP aminoacid sequence in the occurring in nature existence.

Term " homology " refers to that each nucleic acid molecule or encoded protein matter are equivalent on function and/or structure.With the described nucleic acid molecule homology of preamble and for the nucleic acid molecule of the derivative of described nucleic acid molecule is the variant of described nucleic acid molecule that for example has the modification of identical biological function, particularly coding has the proteinic variant of identical or essentially identical biological function.They can be naturally occurring variants, as derive from the sequence of other plant mutation or species body, or mutant nucleotide sequence.These mutant can be naturally occurring or be obtained by induced-mutation technique.Allelic variant can be the variant of naturally occurring allelic variant and synthetic generation or genetic modification.For example, can be by testing combining or identifying structural equivalents of described polypeptide and antibody based on computer forecast.Structural equivalents has similar immunological characteristic, for example contains similar epi-position.

One of polypeptide shown in sequence chart 1a of the present invention, 1b, 1c or the 1d is by substituting, insert or disappearance deutero-functional equivalent and one of polypeptide shown in sequence chart 1a of the present invention, 1b, 1c or the 1d have at least 30%, 35%, 40%, 45% or 50%, preferred at least 55%, 60%, 65% or 70%, preferred at least 80%, preferred especially at least 85% or 90%, 91%, 92%, 93% or 94%, preferred at least 95%, 97%, 98% or 99% homology extremely, and by with the essentially identical characteristic differentiation of the polypeptide shown in sequence chart 1a, 1b, 1c or the 1d.

As sequence chart 1a of the present invention, 1b, nucleotide sequence shown in 1c or the 1d is by substituting, insert or lack one of polypeptide shown in deutero-functional equivalents and the SEQ ID NO:YYY of the present invention and have at least 30%, 35%, 40%, 45% or 50%, preferably at least 55%, 60%, 65% or 70%, preferably at least 80%, especially preferably at least 85% or 90%, 91%, 92%, 93% or 94%, extremely preferably at least 95%, 97%, 98% or 99% homology, and coding and sequence chart 1a, 1b, polypeptide shown in 1c or the 1d has the polypeptide of essentially identical characteristic.

Functional equivalents " essentially identical characteristic " at first is interpreted as functional equivalents and has activity mentioned above, for example, increase the amount of fine chemicals improving described functional equivalents when biology is as protein mass, activity or function in microorganism, plant or plant or animal tissues, plant or zooblast or its part.

" hybridization " refers to that these nucleic acid molecule hybridize under the conventional hybridization condition, preferably (Sambrook " Molecular Cloning for example under stringent condition; A Laboratory Manual ", second edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1989) or " Current Protocols in Molecular Biology ", John Wiley﹠amp; Sons, N.Y. (1989), the stringent condition described in the 6.3.1-6.3.6.) hybridization.

According to the present invention, the DNA of nucleic acid of the present invention and RNA molecule can be used as probe and use.In addition, can be used as the template of identifying the function homologue and carry out Northern trace mensuration and Southern trace mensuration.The Nouthern trace is provided by favourable other information that provide about the gene product of expressing: for example express the generation of graphic, procedure of processing (as montage and add cap) etc.Southern trace mensuration provides about the chromosomal localization of the gene of code book invention nucleic acid molecule and the extraneous information of tissue.

The preferred limiting examples of stringent hybridization condition is under about 45 ℃, 6 * sodium chloride/sodium citrate (=SSC) in hybridization, then at 50 to 60 ℃, for example in 0.2 * SSC, 0.1%SDS, carry out the one or many washing step under 50 ℃, 55 ℃ or 60 ℃.The technician knows these hybridization conditions as the function of nucleic acid type and change, and (for example when having organic solvent) is relevant with the temperature and the concentration of damping fluid.Temperature under " standard hybridization conditions " is as the function of nucleic acid type and change, for example 0.1 *, 0.5 *, 1 *, 2 *, 3 *, 4 * or the aqueous buffer solution of 5 * SSC concentration in (pH7.2) between 42 ℃ and 58 ℃, preferably between 45 ℃ and 50 ℃.When having organic solvent (for example 50% methane amide) in the aforementioned damping fluid, the temperature under the standard conditions is about 40 ℃, 42 ℃ or 45 ℃.The hybridization conditions of DNA:DNA heterozygote for example is preferably 0.1 * SSC and 20 ℃, 25 ℃, 30 ℃, 35 ℃, 40 ℃ or 45 ℃, between preferred 30 ℃ and 45 ℃.The hybridization conditions of DNA:RNA heterozygote for example is preferably 0.1 * SSC and 30 ℃, 35 ℃, 40 ℃, 45 ℃, 50 ℃ or 55 ℃, between preferred 45 ℃ and 55 ℃.The hybridization temperature that preamble is mentioned with for example when the no methane amide about 100bp of length (=base pair) and G+C content be that 50% nucleic acid is determined.By means of textbook or following textbook as the aforementioned, the technician understands how to determine required hybridization conditions: Sambrook etc., " Molecular Cloning ", Cold Spring Harbor Laboratory, 1989; Hames and Higgins compiles 1985, " Nucleic Acids Hybridization:A PracticalApproach ", IRL Press at Oxford University Press, Oxford; Brown compiles 1991, " Essential Molecular Biology:A Practical Approach ", IRL Press atOxford University Press, Oxford.

Other examples of one of these stringent hybridization conditions are at 65 ℃, hybridize among 4 * SSC, then wash one hour in 0.1 * SSC at 65 ℃.Representative stringent hybridization condition in addition be in 50% methane amide, 42 ℃ of following 4 * SSC.In addition, the condition of washing step can be selected in the condition and range that is limited by low stringency condition (about 2 * SSC, 50 ℃) and high stringent condition (about 0.2 * SSC, 50 ℃, preferred 65 ℃) (20 * SSC:0.3M Trisodium Citrate, 3M sodium-chlor, pH7.0).In addition, the temperature of washing step can be brought up to about 65 ℃ of high stringent condition from the room temperature (about 22 ℃) of low stringency condition.Two parameters of salt concn and temperature can change simultaneously, or in two parameters one keeps constant and has only another variation.Also can in hybridization, use denaturing agent, for example methane amide or SDS.When having 50% methane amide, preferably hybridize at 42 ℃.Correlative factor such as i) handle length, ii) the salt condition, iii) the washing composition condition, iv) competitor dna, v) temperature and vi) the selection of probe can make up one by one, so this paper can not mention all possibilities.

Therefore, in preferred embodiments, Northern trace and Rothi-Hybri-Quick damping fluid (Roth, Karlsruhe) were 68 ℃ of prehybridizations 2 hours.Spend the night with radiolabeled probe hybridization at 68 ℃.Then carry out washing step with 1 * SSC at 68 ℃.

Measure for the Southern trace, film and Rothi-Hybri-Quick damping fluid (Roth, Karlsruhe) were 68 ℃ of prehybridizations 2 hours.Cross liquid at 68 ℃ with radiolabeled probe hybridization.Then abandon hybridization buffer, use 2 * SSC, 0.1%SDS washing nozzle in short-term.Add new 2 * SSC, 0.1%SDS damping fluid after abandoning lavation buffer solution, and hatched 15 minutes at 68 ℃.This washing step carries out twice, then uses 1 * SSC, 0.1%SDS to carry out 10 minutes extra washing steps at 68 ℃.

DNA hybridization (Southern trace mensuration) and some other example of washing step are as follows:

(1) hybridization conditions can be selected from for example following condition:

a)4×SSC、65℃，

b)6×SSC、45℃，

C) the milt dna fragmentation of 6 * SSC, 100mg/ml sex change, 68 ℃,

D) salmon sperm DNA of 6 * SSC, 0.5%SDS, 100mg/ml sex change, 68 ℃,

E) the salmon sperm DNA fragment of 6 * SSC, 0.5%SDS, 100mg/ml sex change, 42 ℃,

F) 50% methane amide, 4 * SSC, 42 ℃,

G) 50% (volume/volume) methane amide, 0.1% bovine serum albumin, 0.1% glycan body, 0.1% polyvinylpyrrolidone, 50mM sodium phosphate buffer (pH6.5), 750mM sodium-chlor, 75mM Trisodium Citrate, 42 ℃,

H) 2 * or 4 * SSC, 50 ℃ (low stringency condition), or

I) 30 to 40% methane amides, 2 * or 4 * SSC, 42 ℃ (low stringency condition)

(2) wash conditions can be selected from for example following condition:

A) 0.015M sodium-chlor/0.0015M Trisodium Citrate/0.1%SDS, 50 ℃,

b)0.1×SSC、65℃，

c)0.1×SSC、0.5％SDS、68℃，

D) 0.1 * SSC, 0.5%SDS, 50% methane amide, 42 ℃,

e)0.2×SSC、0.1％SDS、42℃，

F) 2 * SSC, 65 ℃ (low stringency condition).

According to of the present invention, " conversion " is meant plant or its part that all produce and/or modify by working method, wherein

A) one or more genes, preferably by one or more sequence chart 1a, 1b, 1c or the described nucleotide sequence of 1d sequence or its homologue coding, or

B) with the functional genetic regulatory element that is connected of nucleotide sequence (for example promotor) of for example sequence chart 1a, 1b, 1c or 1d sequence or its homologue, or

C) (a) and (b)

Be present in be not it/their natural genotypic environment in or modify through genetic manipulation method.

Modification can by for example substitute, interpolation, disappearance, inversion or insert one or more Nucleotide.

In the present invention, operation is intended to comprise the whole variations in the vegetable cell, comprise induction type or non-induction type (spontaneous) mutagenesis, by traditional breeding or the directed or nondirectional genetic manipulation by modern genetic manipulation method, as interfering (dsRNAi) by double-stranded RNA, introduce antisense nucleic acid, ribozyme, antisense nucleic acid with the ribozyme combination, coding suppresses the nucleic acid of son altogether, the nucleic acid of coding dominance negative regulator matter, described gene of target or RNA or protein DNA or RNA or the protein bound factor, induce the viral nucleic acid and the expression system of RNA degraded, induce the system of described dna homolog reorganization, the sudden change of described gene or the combination of above content reduce genetic expression.

Other modification and working method are apparent by further describing.

" natural genotypic environment " is meant the natural dyeing position point in playing eozoan or is present in the genomic library.With regard to genomic library, the natural genotypic environment of nucleotide sequence preferably remains unchanged to small part.Environment and nucleotide sequence link to each other and have the 50bp sequence length at least in a side at least, preferred 500bp at least, especially preferred 1000bp at least, especially especially preferably 5000bp at least.

If plant or vegetable cell are to isozygoty in the heredity for a concrete proterties,, think that then it is " purebred " to such an extent as to when purebred plant self-pollination, do not observe this proterties of filial generation and freely separate significantly.

Term used herein " nucleic acid " and " nucleic acid molecule " are intended to comprise dna molecular (for example cDNA or genomic dna) and RNA molecule (for example mRNA) and use the DNA of nucleotide analog deposits yields and the analogue of RNA.This term comprises the non-translated sequence that is positioned at coding region 3 ' and 5 ' end equally: gene coding region 5 ' end upstream sequence at least about 1000 Nucleotide and 3 ' end downstream sequence at least about 200 Nucleotide.Nucleic acid molecule can be strand or two strands, but is preferably double-stranded DNA.

" isolating " nucleic acid molecule be with the natural origin that is present in this nucleic acid in other nucleic acid molecule isolated nucleic acid molecule basically.The amount that this means other nucleic acid molecule preferably less than 2% of weight, is more preferably less than 1% of weight less than 5% of purpose nucleic acid weight, most preferably less than 0.5% of weight." isolating " nucleic acid does not preferably contain the nucleic acid biological naturally occurring sequence in genomic dna amplifying nucleic acid both sides (as being positioned at nucleic acid 5 ' and the 3 ' sequence of holding) of originating.For example, it is naturally occurring less than about 5kb, 4kb, 3kb, 2kb, 1kb, 0.5kb or 0.1kb nucleotide sequence that the nucleic acid molecule that separated coding is coerced related protein in different embodiments can contain the genomic dna amplifying nucleic acid molecule both sides of nucleic acid institute derived cell.In addition, " isolating " nucleic acid molecule such as cDNA molecule can not contain other its natural bonded intracellular organic matter, or the substratum when producing by recombinant technology, or precursor or other chemical reagent during chemosynthesis.

Can use standard molecular biological technique and sequence information provided herein separate nucleic acid molecule of the present invention (for example coding when inactivation or down timing gene or its a part of nucleic acid molecule to the patience and/or the resistance of environment-stress are provided in plant).For example, can use all or part of cDNA that from the Arabidopis thaliana library, separates the arabidopsis gene coding of nucleotide sequence shown in sequence chart 1a, 1b, 1c or the 1d.In addition, all or part of nucleic acid molecule that comprises sequence chart 1a, 1b, 1c or 1d sequence can separate based on the polymerase chain reaction of the Oligonucleolide primers of these sequences Design by use.For example can be from vegetable cell separating mRNA (for example by Chirgwin etc., the guanidine thiocyanate extracting method of 1979Biochemistry 18:5294-5299) and can use the ThermoScript II (for example can be available from the Moloney MLV ThermoScript II of Gibco/BRL, Bethesda, MD or can be available from Seikagaku America, Inc., St.Petersburg, the AMV ThermoScript II of FL) preparation cDNA.Can be designed for the synthetic Oligonucleolide primers of polymerase chain reaction based on the nucleotide sequence that shows among sequence chart 1a, 1b, 1c or the 1d.Can use as the cDNA of template or genomic dna and suitable Oligonucleolide primers according to the Standard PC R amplification technique nucleic acid molecule of the present invention that increases.Kuo Zeng nucleic acid molecule can be cloned into appropriate carriers like this, and describes its feature by dna sequence analysis.In addition, can be by the corresponding oligonucleotide of standard synthetic technology (for example using automatic dna synthesizer) preparation genes encoding nucleotide sequence.

In preferred embodiments, isolated nucleic acid molecule of the present invention contains the one section nucleotide sequence (i.e. " coding region ") of code displaying SRP among sequence chart 1a, 1b, 1c or the 1d, and 5 ' non-translated sequence and 3 ' non-translated sequence.

In addition, nucleic acid molecule of the present invention can only contain the part in one section sequence encoding district shown in sequence chart 1a, 1b, 1c or the 1d, for example can be used as the fragment of probe or primer use or the fragment of encoding gene biologically-active moiety.

The gene of genes encoding nucleic acid molecule encoding of the present invention or protein portion are preferably and are gene as herein described or proteinic biologically-active moiety.The gene of the described genes encoding of term used herein or proteinic " biologically-active moiety " are intended to comprise in the plant and participate in gene or the part of protein (preferably realizing by changing metabolic activity), for example zone/motif that stress tolerance and/or resistance are replied.For whether the inactivation of the gene of genes encoding described in the plant identification or protein or its biologically-active moiety or downward modulation cause the stress tolerance (preferably realizing by changing metabolic activity) that improves, can change proteinic plant and coerce analysis containing by above-mentioned screening method for example.The encoding part (as passing through in-vitro recombination expression) of part, expressing gene, protein or polypeptide that more particularly, can be by one of the nucleotide sequence shown in separation sequence Fig. 1 a, 1b, 1c or the 1d or its homologue sequence and the activity of assessment gene, protein or peptide coding part prepare the gene of genes encoding as described in the coding or the nucleic acid fragment of proteinic biologically-active moiety.

In addition, can compare the conservative region of identifying in the multiple biology, therefrom can draw conservative region and degenerated primer by carrying out protein sequence with the employed polypeptide of the inventive method (peptide sequence particularly of the present invention).The conservative region of polypeptide of the present invention has been pointed out in comparison shown in the figure.Conservative region is the zone of the amino acid changes little of a specific position in several homologues of different sources.

Common proteinic part is contained in the present invention, and comprise the aminoacid sequence deutero-aminoacid sequence of one of sequence chart 1a, 1b, 1c or 1d sequence encoded protein matter, or with the proteinic aminoacid sequence of this protein homology, described homologous sequence is compared with full length protein or with the full length protein of protein homology and is comprised less amino acid, and shows proteinic some enzymic activity at least.The preferred part according to the present invention (for example length be 5,10,15,20,30,35,36,37,38,39,40,50,100 or the more polypeptide or the protein of amino acids) comprise the zone/motif that has at least some these protein actives.In addition, can lack the biologically-active moiety of proteinic other parts and assess one or more activity as herein described by the recombinant technology preparation.Preferred protein biologically-active moiety comprises selected one or zone/motif or its part of several biologically actives.

Except protein fragments as herein described, the present invention includes the homologue or the analogue of naturally occurring protein and protein coding nucleic acid in the plant.

" homologue " is defined as two nucleic acid or the protein that has similar or " homologous " Nucleotide or aminoacid sequence respectively in this article.Homologue comprises proteinic allelic variant, lineal homologue, collateral line homologue, agonist or the antagonist of hereinafter definition.Term " homologue " comprises that in addition the degeneracy owing to genetic code is different from and one of the nucleotide sequence shown in sequence chart 1a, 1b, 1c or the 1d (and part), and thereby the nucleic acid molecule of the same protein of encoding amino acid sequence coding." naturally occurring " used herein refers to the aminoacid sequence in the occurring in nature existence.

Except fragment of the present invention described herein and fusion polypeptide, the present invention includes the protein coding nucleic acid of the present invention in naturally occurring proteinic homologue and analogue and the plant." homologue " is defined as two nucleic acid or the polypeptide that has similar or essentially identical Nucleotide or aminoacid sequence respectively in this article.Homologue comprises allelic variant, lineal homologue, collateral line homologue, agonist and the antagonist as the SRP that hereinafter defines.The degeneracy that term " homologue " comprises in addition owing to genetic code is different from the nucleotide sequence (and part) that sequence chart 1a, 1b, 1c or 1d show, and the nucleic acid molecule of the nucleotide sequence coded identical SRP that therefore shows with sequence chart 1a, 1b, 1c or 1d." naturally occurring " used herein protein refers to the aminoacid sequence in the occurring in nature existence.Naturally occurring its minimizing or disappearance cause that the protein that environment-stress is improved patience preferably contains and are selected from amino acid sequence of polypeptide shown in sequence chart 1a, 1b, 1c or the 1d.

Its minimizing or disappearance cause that the proteinic agonist to environment-stress raising patience can keep the identical substantially biological activity of described protein or its part.Described proteinic antagonist can suppress its minimizing or disappearance causes or the multinomial activity that environment-stress is improved the proteinic natural existence form of patience.For example, antagonist can combine with the upstream that comprises described proteinic cell membrane component metabolism cascade or downstream member are competitive, or with the protein bound of the present invention of mediation compound transmembrane transport, thereby stop the generation of transhipment.

Natural allelic variant and the analogue of protein cDNA of the present invention, the corresponding nucleic molecule of lineal homologue and collateral line homologue can use described proteinic cDNAs or its part as hybridization probe, is separating for the identity of yeast saccharomyces cerevisiae described herein, intestinal bacteria, colea, soybean or rice protein nucleic acid based on them under stringent hybridization condition according to the standard hybridization technique.In another embodiment, can cause the proteinic homologue that environment-stress is improved patience by its minimizing of activity identification or the disappearance of protein agonist as described in screening described protein mutant (as the truncated mutant) combinatorial library or antagonist.In one embodiment, its minimizing or disappearance cause the combinatorial mutagenesis generation of the variegated library of the protein variant of environment-stress raising patience being passed through nucleic acid level, and by variegated gene library coding.The generation in the variegated library of SRP variant can thereby gene order makes the degeneracy collection of potential SRP sequence can be used as single expression of polypeptides by for example synthetic oligonucleotide mixture enzyme being connected into, or causes the bigger fusion polypeptide expression (for example phage display) that environment-stress is improved the protein sequence collection of patience as containing its minimizing or disappearance in addition.Can use several different methods to produce the library of potential protein homologue from degenerate oligonucleotide sequence.Can in automatic dna synthesizer, carry out the chemosynthesis of degeneracy gene order, then the synthetic gene be connected into suitable expression vector.The degeneracy collection permission of use gene provides the full sequence of the protein sequence collection potential of the present invention of coding needs in a mixture.The method of synthetic degenerate oligonucleotide is conventionally known to one of skill in the art.Consult for example Narang, S.A., 1983, Tetrahedron 39:3; Itakura etc., 1984, Annu.Rev.Biochem.53:323; Itakura etc., 1984, Science 198:1056; Ike etc., 1983, Nucleic Acid Res.11:477.

In addition, protein coding region fragment of the present invention library can be used to produce protein fragments variegated group with screening and then select described proteinic homologue.In one embodiment, can by under the condition that only each molecule is produced an otch with double-stranded PCR fragment, denatured double stranded dna, the renaturation DNA of nuclease treatment S RP encoding sequence form double-stranded DNA (can comprise from the product with different incisions have justice/antisense to), by the S1 nuclease handle from the pairing that forms again, remove strand partly, connect the fragment library that obtains into that expression vector produces the segmental library of encoding sequence.By this method can obtain encoding segmental between two parties expression library of N-end, C-end and all size of SRP.

Some technology that are used to screen the gene product of the combinatorial library that point mutation or brachymemma produce and have the gene product of selected characteristics from the DNA library screening are well known in the art.These technology are suitable for the gene library of rapid screening by the combinatorial mutagenesis generation of SRP homologue.The broad range of techniques that is suitable for high throughput analysis of screening that big gene library uses generally comprises to be cloned gene library into reproducible expression vector, the vector library that obtains is transformed into suitable cell, and is convenient to separate encode in required active detection and expresses combination gene under the condition of carrier of the product gene that detects.Improve new technology---the overall mutagenesis that circulates (the Reaursive ensemble mutagenesis of function mutation body frequency in the library, REM) can be used in combination to identify SRP homologue (Arkin and Yourvan with identifying SRP screening assay method, 1992, PNAS 89:7811-7815; Delgrave etc., 1993, PolypeptideEngineering 6 (3): 327-331).In another embodiment, can use method well known in the art exploitation based on the assay method of cell to analyze variegated SRP library.The present invention provides the method for identifying new SRP in addition, comprises that (a) obtains to respond to SRP described herein or its segmental specific antibody; (b) the SRP material of inferring with antibody screening, wherein the specific combination of antibody and material represents to exist the new SRP of potential; And (c) and known SRP comparative analysis bonded material, to identify its novelty.

Pointed as preamble, the present invention includes its reduction or disappearance and cause protein and the homologue thereof that environment-stress is improved patience.For determining the sequence identity percentage ratio of two aminoacid sequences (for example one of sequence chart 1a, 1b, 1c or 1d sequence and its mutant forms), purpose with the best comparison sequence is compared (for example for the comparison of the best of a polypeptide or nucleic acid, can in another peptide sequence, introduce breach).Follow the amino-acid residue of more corresponding amino acid position.When the position in the sequence (for example one of sequence of sequence chart 1a, 1b, 1c or 1d) by with another sequence (for example being selected from the mutant forms of the sequence of sequence chart 1a, 1b, 1c or 1d polypeptide) corresponding position on identical amino-acid residue when occupying, two molecules are consistent on this position.Can between two nucleotide sequences, carry out the comparison of same-type.

The sequence identity percentage ratio of two sequences is functions (being number/total number of positions * 100 of sequence identity percentage ratio=consistent position) of the number of the consistent position of sharing between sequence.The isolating amino acid homology thing that the present invention includes preferably with the amino acid complete sequence shown in sequence chart 1a, 1b, 1c or the 1d have at least about 50-60%, preferably at least about 60-70%, more preferably at least about 70-75%, 75-80%, 80-85%, 85-90% or 90-95%, and most preferably at least about 96%, 97%, 98%, 99% or higher identity.In another embodiment, the isolating amino acid homology thing that the present invention includes and the complete amino acid sequence of the nucleic acid sequence encoding shown in sequence chart 1a, 1b, 1c or the 1d have at least about 50-60%, preferably at least about 60-70%, more preferably at least about 70-75%, 75-80%, 80-85%, 85-90% or 90-95%, and most preferably at least about 96%, 97%, 98%, 99% or higher identity.In other embodiments, SRP amino acid homology thing has among sequence chart 1a, 1b, 1c or the 1d and to surpass at least 15 continuous amino acid residues, more preferably at least 25 continuous amino acid residues, the sequence identity of at least 35 continuous amino acid residues most preferably.

In another embodiment preferred, isolating nucleic acid homologue of the present invention contain with the nucleotide sequence shown in sequence chart 1a, 1b, 1c or the 1d or its part that contains at least 20,30,40,50,60 continuous nucleotides have at least about 50-60%, preferably at least about 60-70%, more preferably at least about 70-75%, 75-80%, 80-85%, 85-90% or 90-95%, and most preferably at least about 96%, 97%, 98%, 99% or the nucleotide sequence of higher identity.The length of nucleotide sequence comparative optimization is at least 75 Nucleotide, more preferably at least 100 Nucleotide and the complete length of coding region most preferably.

Preferred in addition isolating nucleic acid homologue coding of the present invention has at least 85% identity with the aminoacid sequence of sequence chart 1a, 1b, 1c or 1d, and brings into play SRP or its part of environment-stress reaction regulon function in plant.In a more preferred embodiment, crossing of plant amplifying nucleic acid homologue expressed the patience of raising plant to environment-stress.

For the present invention, use Vector NTI 6.0 (PC) software package (Bethesda, MD 20814 for InforMax, 7600Wisconsin Ave.) to measure the sequence identity percentage ratio between two nucleic acid or the peptide sequence.It is 15 that the breach that uses when measuring the identity percentage ratio of two nucleic acid is opened point penalty, and it is 6.66 that breach extends point penalty.Identify that it is 10 that breach that two polypeptide identity percentage ratios use is opened point penalty, it is 0.1 that breach extends point penalty.Every other parameter setting is its default setting.For multiple ratio to (Clustal W algorithm), it is 10 that breach is opened point penalty, it is 0.05 of 0.05 blosum62 matrix that breach extends point penalty.Should be appreciated that with regard to determining sequence identity, during with dna sequence dna and the comparison of RNA sequence, thymidylic acid is equal to uridylate.

On the other hand, the invention provides the isolating nucleic acid that contains with the polynucleotide of the polynucleotide hybridize under stringent condition of sequence chart 1a, 1b, 1c or 1d.More specifically, isolated nucleic acid molecule length of the present invention is at least 15 Nucleotide, and under stringent condition with contain the making nucleic acid molecular hybridization of sequence chart 1a, 1b, 1c or 1d nucleotide sequence.In another embodiment, length of nucleic acid molecule is at least 30,50,100,250 or polynucleotide more.The homologue of preferred isolating nucleic acid of the present invention contains the nucleotide sequence with the nucleotide sequence hybridize under stringent condition of sequence chart 1a, 1b, 1c or 1d, and in plant as stress tolerance regulon performance function.In other embodiment preferred, crossing of isolating nucleic acid homologue expressed the patience of raising plant to environment-stress in the plant.

For the hybridization of DNA to southern blotting technique, term used herein " stringent condition " refers to spend the night in 60 ℃ of hybridization in the salmon sperm DNA of 10 * Denharts solution, 6 * SSC, 0.5%SDS and 100 μ g/ml sex change in one embodiment.At 62 ℃ with 3 * SSC/0.1%SDS, then with 1 * SSC/0.1%SDS, use 0.1 * SSC/0.1%SDS to wash trace in each 30 minutes at last." high stringent condition " used herein refers to spend the night in 65 ℃ of hybridization in the salmon sperm DNA of 10 * Denharts solution, 6 * SSC, 0.5%SDS and 100 μ g/ml sex change simultaneously.At 65 ℃ with 3 * SSC/0.1%SDS, then with 1 * SSC/0.1%SDS, use 0.1 * SSC/0.1%SDS to wash trace in each 30 minutes at last.The method of nucleic acid hybridization is described in Meinkoth and Wahl, 1984, volume such as Anal.Biochem.138:267-284, Ausubel, 1995, " Current Protocols inMolecular Biology ", the 2nd chapter, Greene Publishing and Wiley-Interscience, New York and Tijssen, 1993, " Laboratory Techniques in Biochemistry andMolecular Biology:Hybridization with Nucleic Acid Probes ", the 1st one, the 2nd chapter, Elsevier, New York.Preferably, the isolated nucleic acid molecule of the present invention of hybridizing under strict or high stringent condition with sequence chart 1a, 1b, 1c or 1d sequence is corresponding to naturally occurring nucleic acid molecule.The nucleic acid molecule of " natural existence " used herein refers to have RNA or the dna molecular that there is the nucleotide sequence of (natural polypeptides of for example encoding) in occurring in nature.In one embodiment, the naturally occurring yeast saccharomyces cerevisiae of nucleic acid encoding, intestinal bacteria, colea, soybean or rice SRP.

Use preceding method and additive method well known by persons skilled in the art, those of ordinary skills can separate the homologue of the SRP that contains the aminoacid sequence shown in sequence chart 1a, 1b, 1c or the 1d.A subclass of these homologues is an allelic variant.Term used herein " allelic variant " refers to contain the nucleotide sequence of the polymorphism that causes that the SRP aminoacid sequence changes, and this polymorphism exists in natural population (for example plant species or mutation).These natural equipotentials change the variation that causes 1-5% in the SRP nucleic acid usually.Allelic variant can be identified by in a large amount of different plants the purpose nucleotide sequence being checked order, can be convenient to use hybridization probe and identify SRP gene locus identical in those plants.The invention is intended to comprise this arbitrary and whole class nucleic acid variations, and as natural allelic variation and do not change the result of SRP functionally active and amino acid polymorphism or variation among the SRP that produces.

Can be by in the nucleotide sequence of sequence chart 1a, 1b, 1c or 1d, introducing replacement, interpolation or the disappearance of one or more Nucleotide respectively, and thereby in encoded polypeptides, introduce one or more amino acid and replace, add or disappearance and produce the isolated nucleic acid molecule that coding and sequence chart 1a, 1b, 1c or 1d peptide sequence have the SRP of sequence identity.Can pass through standard technique and (in the sequence of sequence chart 1a, 1b, 1c or 1d, introduce sudden change as directed mutagenesis and PCR mediated mutagenesis.Preferably, carrying out conservative amino acid at the non-essential amino acid residue place of one or more predictions replaces." conservative amino acid replacement " is the replacement with the amino-acid residue substituted amino acid residue with similar side chain.

Preferably knock out sudden change in necessary site.

This area has defined the amino-acid residue family with similar side chain.These families comprise amino acid with basic side chain (Methionin for example, arginine, Histidine), amino acid (Aspartic Acid for example with acid side-chain, L-glutamic acid), amino acid (glycine for example with uncharged polar side chain, l-asparagine, glutamine, Serine, Threonine, tyrosine, halfcystine), amino acid (L-Ala for example with non-polar sidechain, Xie Ansuan, leucine, Isoleucine, proline(Pro), phenylalanine, methionine(Met), tryptophane), amino acid (Threonine for example with β branched building block, Xie Ansuan, Isoleucine) and have the amino acid (tyrosine for example of aromatic side chain, phenylalanine, tryptophane, Histidine).Therefore, the non-essential amino acid residue of predicting among the SRP is preferably by the aminoacid replacement from same side chain family.In addition, in another embodiment, can be as in all or part of SRP encoding sequence, introducing sudden change at random by saturation mutagenesis, the SRP activity as herein described that can screen the gained mutant keeps the active mutant of SRP to identify.After one of sequence of sequence chart 1a, 1b, 1c or 1d carried out mutagenesis, can recombinant expressed encoded polypeptides and can be as described herein stress tolerance by analyzing the plant of expressing this polypeptide to identify the activity of polypeptide.

Except encode aforementioned its reduction or disappearance cause environment-stress is improved the proteinic nucleic acid molecule of patience, another aspect of the present invention is relevant with its antisense nucleic acid molecule.Antisense polynucleotides is considered to by with the target polynucleotide specific combination and disturb the transcribing of target polynucleotide, montage, transhipment, translation and/or stability to suppress the genetic expression of target polynucleotide.Method with the mRNA of antisense polynucleotides targeting staining body DNA, primary rna transcript or processing has been described in the prior art.Preferred target region comprises other sequences in splice site, translation initiation codon, translation stop codon and the open reading-frame (ORF).

Term of the present invention " antisense " refers to contain to be enough to and the mRNA complementation of all or part of, transcript or the processing originally of the gene nucleic acid with the polynucleotide that disturb native gene and express." complementation " polynucleotide can form base pairing according to standard Watson-Crick principle of complementarity.Particularly, the pairing of purine and pyrimidine bases forms combination, guanine match (G:C) with cytosine(Cyt) and VITAMIN B4 and thymus pyrimidine (in DNA) (A:T) or uridylic (in RNA) (A:U) match.Should be appreciated that, as long as two polynucleotide respectively contain at least one and the basic complementary of another polynucleotide zone, even their complete each other complementations also can be hybridized mutually.Term " antisense nucleic acid " comprises single stranded RNA and can transcribe the double-stranded DNA that produces sense-rna and express box." activity " antisense nucleic acid be can with the antisense rna molecule of the mRNA selective cross of transcript or coded polypeptide originally, the polypeptide of described polypeptide and sequence chart 1a, 1b, 1c or 1d has at least 80% sequence identity.

Antisense nucleic acid can be complementary or only complementary with its part with complete S RP coding strand.In one embodiment, " coding region " antisense of the coding strand of the nucleotide sequence of antisense nucleic acid molecule and coding SRP.Term " coding region " refers to contain the nucleotide sequence district of the codon of translating into amino-acid residue.In another embodiment, " non-coding region " antisense of the nucleotide sequence coded chain of antisense nucleic acid molecule and coding SRP.Term " non-coding region " refers to that the both sides, coding region do not translate into amino acid whose 5 ' and 3 ' sequence (promptly being also referred to as 5 ' and 3 ' non-translational region).Antisense nucleic acid molecule can with the complete coding region complementation of SRP mRNA, but more preferably only with the oligonucleotide of a part of antisense of SRP mRNA coding region or non-coding region.For example, antisense oligonucleotide can with PKSRP mRNA antisense initiator site around regional complementarity.The length of antisense oligonucleotide can be for example about 5,10,15,20,25,30,35,40,45 or 50 Nucleotide.Usually, antisense molecule of the present invention contains the RNA that at least 14 continuous nucleotides of one of nucleic acid with sequence chart 1a, 1b, 1c or 1d have 60-100% sequence identity.Preferred sequence identity is at least 70%, more preferably 75%, 80%, 85%, 90%, 95%, 98% and most preferably 99%.

Can use methods known in the art, utilize chemosynthesis and enzyme ligation to make up antisense nucleic acid of the present invention.For example, can use the synthetic antisense nucleic acid (for example antisense oligonucleotide) of Nucleotide chemistry of naturally occurring Nucleotide or various modifications, the Nucleotide that designs described modification is for the biologically stable that improves molecule or improves antisense and the physical stability of the duplex that forms between phosphorothioate odn is arranged, the Nucleotide that for example can use thiophosphoric acid derivative and acridine to replace.The Nucleotide example that can be used to produce the modification of antisense nucleic acid comprises 5 FU 5 fluorouracil, 5-bromouracil, the 5-chlorouracil, 5-iodouracil, xanthoglobulin, xanthine, the 4-acetylcytosine, 5-(carboxyl hydroxymethyl) uridylic, 5-carboxyl methylamino methyl-2-sulphur uridine, 5-carboxyl methylamino 6-Methyl Uracil, two hydrogen uridylics, β-D-galactosyl queosine, Trophicardyl, the N6-isopentennyladenine, the 1-methyl guanine, the 1-methyl inosine, 2, the 2-dimethylguanine, the 2-methyladenine, the 2-methyl guanine, the 3-methylcystein, 5-methylcytosine, the N6-VITAMIN B4, the 7-methyl guanine, 5-methylamino 6-Methyl Uracil, 5-methoxyl group amino methyl-2-thiouracil, β-D-mannose group queosine, 5 '-methoxyl group carboxyl 6-Methyl Uracil, the 5-methoxyuracil, 2-methylthio group-N6-isopentennyladenine, uridylic-the 5-ethoxyacetic acid (v), wybutoxosine, pseudouracil, queosine, 2-sulfo-cytosine(Cyt), 5-methyl-2-deracil, the 2-deracil, the 4-deracil, methyl uracil, uridylic-5-oxy acetic acid methyl ester, uridylic-the 5-fluoroacetic acid (v), 5-methyl-2-deracil, 3-(3-amino-3-N-2-carboxyl propyl group) uridylic, (acp3) w and 2,6-diaminopurine.In addition, can use with antisense orientation (promptly will be the antisense orientation of purpose target nucleic acid, specific descriptions are hereinafter arranged) the nucleic acid subclone to wherein expression vector deposits yields in next life antisense nucleic acid from inserting the RNA that transcribed nucleic acid produces.

In yet another embodiment, antisense nucleic acid molecule of the present invention is α-end group isomery nucleic acid molecule.α-end group isomery nucleic acid molecule and complementary RNA form special double-stranded heterozygote, and be wherein opposite with conventional β-unit, and two chains are (Gautier C etc., 1987, Nucleic Acids Res 15:6625-6641) parallel to each other.Antisense nucleic acid molecule also can comprise 2 '-O-methyl ribonucleotides (Inoue etc., 1987, Nucleic Acids Res 15:6131-6148) or chimeric RNA-DNA analogue (Inoue etc., 1987, FEBS Lett 215:327-330).

Antisense nucleic acid molecule of the present invention generally is applied to cell or original position and produces, so that it is with cell mRNA and/or the genomic dna hybridization of coding SRP or combine, and for example transcribes and/or translate the inhibition polypeptide expression by inhibition.Can form stable duplex by conventional Nucleotide is complementary, perhaps for example for DNA duplex bonded antisense nucleic acid molecule, hybridize by the special interaction in double-helical major groove.Antisense molecule can be modified so that it combines (for example by being connected on the antisense nucleic acid molecule with cell surface receptor or antigen bonded polypeptide or antibody) with the acceptor or the antigen-specific of selected cell surface expression.Antisense nucleic acid molecule also can use carrier as herein described to be delivered to cell.For making antisense molecule reach enough intracellular concentrations, preferably antisense nucleic acid molecule is placed the vector construction body under the control of strong protokaryon, virus or eucaryon (comprising plant) promotor.

As the replacement of antisense polynucleotides, can use ribozyme, have adopted polynucleotide or double-stranded RNA (dsRNA) to reduce the SRP polypeptide expression." ribozyme " is meant the catalytic enzyme based on RNA with nuclease, and it can cut the single-chain nucleic acid (as mRNA) that has complementary district with it.Ribozyme (for example Haselhoff and Gerlach, 1988, the hammerhead ribozyme of describing among the Nature 334:585-591) can be used for catalytic cutting SRP mRNA transcript to suppress the translation of SRP mRNA.Can as the basis of (being sequence chart 1a, 1b, 1c or 1d) disclosed herein SRP cDNA nucleotide sequence on, or design has specific ribozyme to the SRP encoding gene on according to the basis of the isolating heterologous sequence of method of this paper instruction.For example, can make up the derivative of thermophilas (Tetrahymena) L-19IVS RNA, wherein the nucleotide sequence of avtive spot with will the SRP coding mRNA of cutting in the nucleotide sequence complementation.Consult for example U.S.Patent No.4 of Cech etc., 987,071 and 5,116,742. in addition, and SRP mRNA can be used for selecting the catalytic RNA with special nuclease from the RNA library of molecules.Consult for example Bartel, D. and Szostak, J.W., 1993, Science 261:1411-1418. in preferred embodiments, ribozyme contains at least 7,8,9,10,12,14,16,18 or 20 Nucleotide that have 100% complementarity with the part of target RNA, the more preferably part of 7 or 8 Nucleotide.The method that produces ribozyme is conventionally known to one of skill in the art.Consult for example U.S.Patent No.6,025,167,5,773,260 and 5,496,698.

Term used herein " dsRNA " refers to contain the heterozygote of two RNA chains.The structure of dsRNA term used herein " dsRNA " refers to contain the RNA heterozygote of two RNA chains.The structure of dsRNA can be linear or annular.In preferred embodiments, dsRNA is specific to encoding sequence Fig. 1 a, 1b, 1c or 1d polypeptide or has the polynucleotide of the polypeptide of at least 70% sequence identity with sequence chart 1a, 1b, 1c or 1d polypeptide.The RNA of hybridization can be basic or complete complementary." complementary substantially " refers to when the RNA of two hybridization uses the blast program optimum matching as described above hybridization portion at least 95% complementation.Preferred dsRNA length is at least 100 base pairs.Usually, the RNA of hybridization should be same length, not outstanding 5 ' or 3 ' end and breach.Yet, also can use in the method for the present invention to have 5 ' or the 3 ' dsRNA that gives prominence to that grows to 100 Nucleotide.

DsRNA can contain ribonucleotide or ribonucleoside acid-like substance (as 2 '-O-methylribose base residue) or its combination.Consult for example U.S.Patent No.4,130,641 and 4,024,222.U.S.patent 4,283, described dsRNA multinuclear sugar hypoxanthylic acid in 393: multinuclear sugar cytidylic acid.It is well known in the art producing and using the method for dsRNA.A kind of method comprises in vivo or transcribe two complementary dna chains in the reaction mixture outside individual.Consult for example U.S.Patent No.5,795,715.In one embodiment, can be by the standard conversion method directly with dsRNA introduced plant or vegetable cell.In addition, can in vegetable cell, express dsRNA by transcribing two complementary RNA.

The additive method that suppresses the native gene expression is well known in the art, as forming triple helical (Moser etc., 1987, Science 238:645-650 and Cooney etc., 1988, Science 241:456-459) and suppress (Napoli etc. altogether, 1990, The Plant Cell 2:279-289).Local and total length eDNA has been used for the common inhibition of native gene.Consult for example U.S.Patent No.4,801,340,5,034,323,5,231,020 and 5,283,184; Van der Kroll etc., 1990, The Plant Cell 2:291-299; Smith etc., 1990, Mol.Gen.Genetics 224:477-481 and Napoli etc., 1990, The Plant Cell 2:279-289.

There are adopted polynucleotide can block transcribing of corresponding target genes for having justice to suppress, think to introduce.There are adopted polynucleotide and target plant gene or RNA to have at least 65% sequence identity.Preferred identity percentage ratio is at least 80%, 90%, 95% or higher.The adopted polynucleotide of introducing that have need be corresponding to the total length of target gene or transcript.There are adopted polynucleotide preferably to have at least 65% sequence identity of at least 100 continuous nucleotides with one of nucleic acid of sequence chart 1a, 1b, 1c or 1d.Intron and/or exon and non-translational region can be contained in the evaluation zone.The adopted polynucleotide of introducing that have can instantaneous existence in vegetable cell, or stable integration advances plant chromosome or exosome replicon.

In addition, scope intention of the present invention comprises the nucleic acid molecule (as SRP analogue, lineal homologue and collateral line homologue) from the coding SRP of identical or other species.Term used herein " analogue " refers to have same or similar function, but two nucleic acid of in irrelevant species, evolving respectively.Term used herein " lineal homologue " refers to derive from different plant species, forms two nucleic acid of coming of evolving from the common ancestral gene by species.Usually, the protein of lineal homologue coding with same or similar function.Simultaneously term used herein " collateral line homologue " refers to two nucleic acid of being correlated with by duplicating in the genome.The collateral line homologue has different functions usually, but these functions may be correlated with (Tatusov, 1997Science such as R.L. 278 (5338): 631-637).The naturally occurring analogue of related protein, lineal homologue and the collateral line homologue of coercing can be different from the naturally occurring related protein of coercing by posttranslational modification, difference by aminoacid sequence or the two.Posttranslational modification comprises polypeptide in vivo with external chemically derived, for example acetylize, carboxylation, phosphorylation or glycosylation, these modifications can occur in the synthetic or course of processing of polypeptide or with isolating modifying enzyme with aftertreatment.Particularly, lineal homologue of the present invention and naturally occurring coerce the related protein aminoacid sequence all or part of show 80-85% at least, more preferably 90%, 91%, 92%, 93%, 94%, most preferably 95%, 96%, 97%, 98% or even 99% identity or homology, and show the function similar to coercing related protein.

These homologues, analogue, lineal homologue and collateral line homologue are commonly called homologue or homologous in this application.

The homologue of the sequence that provides among sequence chart 1a, 1b, 1c or the 1d will also be understood that in addition to referring to for example homologue, analogue, lineal homologue and collateral line homologue, it has at least 30% homology (=identity) on the deutero-amino acid levels, preferred at least 50%, 60%, 70% or 80% homology, preferred at least 85% homology, especially preferred at least 90%, 91%, 92%, 93%, 94% homology, 95%, 96%, 97%, 98% or 99% homology most preferably especially.Homology (=identity) is calculated on complete amino acid scope.The program of using is PileUp (J.Mol.Evolution., 25 (1987), 351-360, Higgins etc., CABIOS is 51989:151-153) and as GCG software package [Genetics Computer Group, 575Science Drive, Madison, Wisconsin, USA 53711 (1991)] Gap and the BestFit program of a part [be respectively Needleman and Wunsch (J.Mol.Biol.48; 443-453 (1970) and Smith and Waterman (Ady.Appl.Math.2; 482-489 (1981)).Use following parameter: the breach weight: 8, length weight: 2, on total sequence length, calculate aforementioned sequence homology percentage ratio with BestFit or Gap program (preferred Gap).

In addition, the invention provides the method that transforms plant that produces, the inactivation or the downward modulation that wherein transform gene in the plant cause patience and/or the resistance that compares the environment-stress raising with corresponding unconverted wild-type plant, described raising preferably realizes by changing metabolic activity, this method comprises a) by inactivation or reduces one or more gene-transformed plant cells, described gene is preferably by one or more sequence chart 1a that are selected from, 1b, the nucleic acid encoding of nucleic acid and/or its homologue shown in 1c or the 1d, and b) compare the conversion plant that environment-stress has raising patience and/or resistance from vegetable cell generation and corresponding wild-type plant.

The present invention also comprises induce the method that compares the patience that environment-stress improves with corresponding unconverted wild-type plant by changing metabolic activity in described vegetable cell or described plant, preferably by by inactivation or the downward modulation metabolic activity by the aforementioned metabolite of one or more gene alterations of one or more nucleic acid encodings of the nucleic acid that is selected from sequence chart 1a, 1b, 1c or 1d or its homologue.

Preferred nucleic acid and described sequence (seeing above) have at least about 30%, particularly at least 50% homology.Homologous sequence can also derive from and be selected from corn, wheat, rye, oat, triticale, paddy rice, barley, soybean, peanut, cotton, Semen Brassicae campestris, rape, cassava, pepper, Sunflower Receptacle, flax, the Borrago officinalis, safflower, Semen Lini, Flower of Beltleaf Primrose, Semen Brassicae campestris, turnip rape, Flower of Aztec Marigold, plant of Solanaceae, potato, tobacco, eggplant, tomato, broad bean, pea, clover, coffee, cocoa, tea, Salix, oil palm, coconut, perennial herb, fodder crop and Arabidopis thaliana, swede type rape, the plant of soybean and rice.

Can or reduce described gene by all the method inactivations known to those skilled in the art, preferably interfere (dsRNAi), introducing antisense nucleic acid, ribozyme, antisense nucleic acid, coding nucleic acid, the described gene of nucleic acid, target or RNA or the protein DNA or the protein bound factor of coding dominance negative regulator matter, the viral nucleic acid and the expression system of inducing the RNA degraded, the system of inducing described dna homolog reorganization, the sudden change of described gene or the combination minimizing genetic expression of aforesaid method of inhibition altogether with the ribozyme combination by double-stranded RNA.

Nucleotide sequence of the present invention or its homologue are the isolated nucleic acid sequences of coded polypeptide.Inactivation or reduced these nucleic acid or their encoded polypeptides and their biology and enzymic activity causes resistance and/or patience that environment-stress is improved thus in the method for the invention, and described raising preferably realizes by changing metabolic activity.

In this article, inactivation is meant that in biology or cell (as in plant or vegetable cell) no longer can detect the enzyme or the biological activity of encoded polypeptides.Downward modulation for the present invention, (=reduce) be meant with the biological activity that is untreated and compare, the enzyme of encoded polypeptides or biologically-active moiety ground or reduction substantially fully.This can realize by different cytobiology mechanism.In this article, activity can be in whole biology, or for multicellular organism in the part of biology, for example reducing in the tissue (as seed, leaf, root or other parts) for plant.In this article, at least reduce by 10% with the biophase that is untreated than enzymic activity or biological activity, advantageously at least 20%, preferably at least 30%, especially preferably at least 40%, 50% or 60%, extremely preferably at least 70%, 80%, 90% or 95%, 99% or even 100%.Particularly advantageous embodiment is inactivation nucleic acid or its encoded polypeptides.

The present invention includes the strategy of amount (=expression), activity or the function of multiple reduction nucleic acid encoding protein matter of the present invention.One skilled in the art will realize that and to use a series of diverse ways to influence proteinic quantity, activity or function in the mode of needs.

Preferably realize the reduction of activity or function by the expression of gene that reduces the coding endogenous protein.

Can use following method to reduce protein mass, activity or function:

A) introduce double-stranded RNA nucleotide sequence (dsRNA) or expression cassette or, and guarantee the expression of expression cassette more than an expression cassette;

B) introduce anti sense nucleotide sequence or expression cassette, and guarantee the latter's expression; Comprise method at the anti sense nucleotide sequence of gene (being genomic dna sequence) or genetic transcription thing (being the RNA sequence); Also comprise α-end group isomery nucleotide sequence;

C) introduce with the anti sense nucleotide sequence of ribozyme combination or guarantee the expression cassette that the former expresses;

D) introduce and to be used to induce the phosphorothioate odn sequence being arranged or guaranteeing the former expression expression cassette of common inhibition;

E) introduce the nucleotide sequence or the expression cassette of coding dominance negative regulator matter, and guarantee the latter's expression;

F) introduce at gene, RNA or protein DNA, RNA or the protein bound factor or expression cassette, and guarantee the latter's expression;

G) cause the nucleic acid sequence of RNA degraded or guarantee the expression cassette that the former expresses;

H) introduce the construct of inducing the native gene homologous recombination, for example be used to produce the construct that knocks out mutant;

I) introduce sudden change to produce afunction (for example producing terminator codon, reading frame shift etc.) to native gene.

In these methods each can cause the reduction of expression, activity or function for the object of the invention.Also can be used in combination.Other method is conventionally known to one of skill in the art, and can comprise the transhipment that hinders or stop proteinic processing, protein or its mRNA, the enzyme that the inhibition rrna adheres to, suppresses RNA montage, induced degradation RNA and/or extension and the termination that inhibition is translated.

Term " protein mass " refers to the quantity of polypeptide in biology, tissue, cell or the cellular compartment." reduction " of term protein amount refers to and compare the quantitative reduction of protein quantity in biology, tissue, cell or the cellular compartment without belonging to together with wild-type of the same race of present method (a kind of method as mentioned below) processing when other conditions (for example culture condition, plant age etc.) is identical.In this article, with the biophase ratio that is untreated, at least 10%, advantageously at least 20%, preferably at least 30%, especially preferably at least 40%, 50% or 60%, extremely preferred at least 70%, 80%, 90% or 95%, 99% or even 100% reduction be favourable.Particularly advantageous embodiment is inactivation nucleic acid or its encoded polypeptides.

Term " activity " preferably refers to the activity of polypeptide in biology, tissue, cell or the cellular compartment.Term active " reduction " refers to and compares without belonging to together with wild-type of the same race of present method (a kind of method as mentioned below) processing when identical (for example culture condition, plant age etc.) in other conditions, the overall active quantitative reduction of protein in biology, tissue, cell or the cellular compartment.In this paper context, with the biophase ratio that is untreated, at least 10%, advantageously at least 20%, preferably at least 30%, especially preferably at least 40%, 50% or 60%, extremely preferred at least 70%, 80%, 90% or 95%, 99% or even 100% activity to reduce be favourable.Particularly advantageous embodiment is inactivation nucleic acid or its encoded polypeptides.

Term " function " preferably refers to the enzyme or the regulatory function of peptide in biology, tissue, cell or the cellular compartment.Particularly advantageous embodiment is a functionally inactive.Suitable substrate is low-molecular weight compound and proteinic protein interaction mating partner." reduction " of term function refers to and compares without belonging to together with wild-type of the same race of present method (a kind of method as mentioned below) processing when identical (for example culture condition, plant age etc.) in other conditions that protein is to the quantitative reduction of at least a substrate binding ability or bonding strength in biology, tissue, cell or the cellular compartment.Reduce the modification that also can be regarded as substrate specificity, described substrate specificity can be by for example k _Cat/ K _mValue is represented.In this article, reduce to be at least 10%, favourable to be at least 20%, preferably at least 30%, especially preferably at least 40%, 50% or 60% than favourable function with the biophase that is untreated, extremely preferably at least 70%, 80%, 90% or 95%.Particularly advantageous embodiment is the inactivation of function.The binding partners of method (for example the passing through yeast two-hybrid system) identification of protein that can be familiar with by the technician.

Be the general introduction of indivedual preferred method below:

A) introduce double-stranded RNA nucleotide sequence (dsRNA)

Method (double-stranded RNA interference by the double-stranded RNA regulatory gene; ((2000) Plant Mol Biol43:401-415 such as Matzke MA for example dsRNAi) existingly in animal and plant biological body is repeatedly described; (1998) Nature 391:806-811 such as Fire A; WO 99/32619; WO 99/53050; WO 00/68374; WO 00/44914; WO 00/44895; WO 00/49035; WO 00/63364).In the above reference of referring to, its process and method have been described clearly.Efficient gene suppresses also to show the result of bio-transformation (for example, as) under the transient expression situation or behind the transient expression (2000) Plant J 200024:895-903 such as () Schweizer P.The dsRNAi method is based on the complementary strand of while quiding gene transcript and the efficient inhibition that corresponding chain causes described genetic expression thereof.The phenotype that is produced with knock out mutant closely similar (1998) Proc Natl Acad Sci USA 95:13959-64 such as () Waterhouse PM accordingly.

(Gens Dev., 1999,13 (24): 3191-3197) efficient that shows the RNAi method is the function that duplex length, 3 ' distal process go out length and these sequence in outstanding to Tuschl etc.Guard between different plant species based on the principle that work and the supposition of Tuschl etc. are emphasized, then can give those skilled in the art following guidance:

For the result who obtains, generally should avoid 5 ' and the 3 ' non-translational region of the nucleotide sequence that uses and the zone of close initiator codon, because the adjusting protein binding site is rich in these zones, RNAi sequence and these interactions of regulating between the protein may cause unwanted interaction;

In plant, 5 ' and 3 ' non-translational region of the nucleotide sequence that uses and the result that can obtain near the zone of initiator codon (preferably at upstream from start codon 50 to 100nt), so should not avoid;

The preferred zone of selecting employed mRNA AUG initiator codon downstream 50 to 100nt (=Nucleotide or base);

Only the dsRNA (=double-stranded RNA) sequence from exon can be used for present method, because do not have effect from the sequence of intron;

G/C content in this zone should be preferably in about 50% greater than 30% and less than 70%;

The possible secondary structure of said target mrna is lower to the effect importance of RNAi method.

Confirmed that the dsRNAi method is especially effectively also favourable to the nucleotide sequence and/or the expression of its homologue that reduce odd number sequence among the SEQ ID No.1-89.Special described in WO 99/32619, the dsRNAi method obviously is better than traditional antisense method.

Therefore the present invention also relates to when introducing in the biology, when favourable is in the introduced plant (or its deutero-cell, tissue, organ or seed), cause that sequence chart 1a, 1b, 1c or 1d nucleotide sequence and/or its homologue express the double stranded rna molecule (dsRNA molecule) that reduces.At the double stranded rna molecule of the protein expression of the nucleic acid sequence encoding that is used for reducing one of sequence chart 1a, 1b, 1c or 1d sequence or its homologue,

I) one of two RNA chains are with basic identical to the small part nucleotide sequence, and

Ii) another RNA chain and complementary strand is basic identical to the small part nucleotide sequence.

Term " basic identical " refers to compare with target sequence, and the dsRNA sequence also can have insertion, disappearance and indivedual point mutation, but still causes effective reduction of expression.Preferably, inhibition dsRNA " sense strand " and nucleotide sequence of the present invention reach at least 75% to (or between complementary strand of " antisense strand " and nucleotide sequence) between the small part sections as the homology of above definition, preferably at least 80%, extremely preferably at least 90%, most preferably 100%.The length of part sections reaches at least 10 bases, preferably at least 25 bases, especially preferably at least 50 bases, extremely preferably at least 100 bases, most preferably at least 200 bases or at least 300 bases.In addition, " essentially identical " dsRNA also may be defined as can with the nucleotide sequence of part storage protein genetic transcription thing hybridization (for example at 400mMNaCl, 40mM PIPES pH 6.4,1mM EDTA carried out 12 to 16 hours in 50 ℃ or 70 ℃).

DsRNA can be made up of one or more polymeric nucleotide chain.In addition, can exist the two the modification of sugar-phosphate backbone and nucleosides.For example, the phosphodiester bond of natural RNA can their modes of comprising at least one nitrogen or sulfur heteroatom be modified.The mode that base can be for example limits the activity of adenosine deaminase is modified.These and further be modified at the method that hereinafter is used for stabilized antisense rna and describe to some extent.

DsRNA can prepare by enzyme, or produces by chemosynthesis wholly or in part.

Can be by for example using the long dsRNA of coli rnase enzyme III (RNaseIII) part digestion the effective effective short dsRNA that the grows to 30bp (Yang of mediate rna interference of generation, D., Deng (2002) Proc.Nati.Acad.Sci.USA 99,9942.).

Double-stranded dsRNA structure can form from single, automatic complementary RNA start of chain or from two complementary RNA start of chains.When the situation of single, automatic complementary strand, there are justice and antisense sequences to connect and form for example hairpin structure by catenation sequence (joint).Preferably, catenation sequence can be intron, and described intron is sheared behind synthetic dsRNA.The nucleotide sequence of coding dsRNA can comprise additional element for example transcription termination signal or polyadenylation signal.If two chains of dsRNA make up in cell or plant, this can take place in many ways, for example:

A) with carrier transformant that comprises two kinds of expression cassettes or plant (favourable is plant);

B) with two carrier cotransfection cells or biology (favourable is plant), of described two carriers comprises the expression cassette that has sense strand and another comprises the expression cassette that has antisense strand;

C) hybridization two biologies (favourable is plant), each in the described biology transforms with carrier, and of described carrier comprises the expression cassette that has sense strand and another comprises the expression cassette that has antisense strand;

D) with after comprising the carrier transformant or biology of the expression cassette that has " antisense " chain, use the carrier excess revolutions cell or the biology (favourable is plant) that comprise the expression cassette that has " justice " chain;

E) introduce the construct comprise two promotors, described promotor causes that the sequence that needs transcribes from both direction; And/or

F) with through design virus transfection cell or biology (favourable be plant), described virus can produce the dsRNA molecule that needs.

The formation of RNA mixture can originate in extracellular or the cell.

If dsRNA is synthetic outside target cell or biology, it can be by injection, microinjection, electroporation, high-velocity particles, introduce biology or biomass cells by laser beam or by compound (DEAE-dextran, calcium phosphate, liposome) mediation, perhaps also can feed the intestinal bacteria of bacterium as (through transforming to express double-stranded RNA i) to animal under the situation of animal.

As shown in WO 99/53050, dsRNA also can be by comprising hairpin structure via " joint " connection " justice " and " antisense " chain (for example intron).Preferred self complementary dsRNA structure is because they only need expression construct and the complementary strand of mol ratio such as always comprise.

As shown in WO 99/53050, dsRNA also can be by comprising hairpin structure via " joint " connection " justice " and " antisense " chain (for example intron).Preferred self complementary dsRNA structure is because they only need expression construct and the complementary strand of mol ratio such as always comprise.

Use method hereinafter described, the expression cassette of preferably will encode dsRNA " antisense " or " justice is arranged " chain or dsRNA self complementary strand is inserted in the carrier and stable (for example using selective marker) is inserted in the Plant Genome, to guarantee the continuous expression of dsRNA.

Can dsRNA be introduced with the amount that makes each at least one copy of cell.Relatively large (for example, each cell at least 5,10,100,500 or 1000 copies) can cause more effective reduction.

As already described, for the effective reduction that causes expression, between the odd number sequencing nucleic acid sequence of dsRNA and SEQ ID No.XXX or the genetic transcription thing of its homologue and nonessential 100% sequence identity.Therefore, advantageously this method can be born the ordering bias that exists as the result of genetic mutation, polymorphism or evolutionary divergence.Therefore, for example produce the dsRNA of one of the sequence of sequence chart 1a, 1b, 1c or 1d in a kind of organism, can for example suppress to express accordingly in another organism.

Sequence homology owing to height between sequence chart 1a, the 1b, 1c or the 1d sequence that derive from multiple biology (for example plant), be protein in other plant for example, may be high conservative, think that therefore being expressed in the other plant species of dsRNA that derives from open sequence shown in sequence chart 1a, 1b, 1c or the 1d or its homologue also may have favourable effect.

DsRNA can be in vivo or is external synthetic., the dna sequence dna of coding dsRNA can be imported in the expression cassette, described expression cassette is under at least one Gene Handling element (for example promotor, enhanser, silencer, donor splicing site or acceptor, polyadenylation signal) control for this reason.Suitable useful construct is described hereinafter to some extent.Polyadenylation is optional, also must be useful on the element of initial translation.

DsRNA can be synthetic with chemistry or enzyme process.For this reason, available cell RNA polysaccharase or phage rna polymerase (for example T3, T7 or SP6RNA polysaccharase).Existing describe (WO 97/32016, US 5,593,874, US 5,698,425, US 5,712,135, US 5,789,214, the US 5,804,693) of appropriate method that is used for the RNA vivoexpression.External by chemistry or enzyme process synthetic dsRNA before importing to cell, tissue or organism, can for example extract, the combination of precipitation, electrophoresis, chromatography or these methods, thereby separate wholly or in part in the reaction mixture.DsRNA can directly import in the cell or by the extracellular and use (for example importing to the intercellular substance).

Yet plant optimization carries out stable conversion with the expression construct that causes the dsRNA expression.Appropriate means is described hereinafter to some extent.

B) introduce anti sense nucleotide sequence

Can be extensive use of to by " antisense " thus technology stops the accumulation of mRNA to suppress the method for specified protein, existing a large amount of description of these methods comprises method (Sheehy etc. (1988) the Proc Natl Acad Sci USA 85:8805-8809 that is used for plant; US 4,801, and 340; (1990) FEBS Lett 268 (2) such as Mol JN: 427-430).The cell mRNA and/or the genomic dna hybridization of the target protein that antisense nucleic acid molecule and coding desire suppress or combine, described hybridization or in conjunction with having suppressed transcribing and/or translating of target protein.Formation by stablizing duplex in traditional way, or hybridize being combined into of described antisense nucleic acid molecule and genomic dna duplex with combining of genomic dna duplex by antisense nucleic acid molecule for genomic dna by special interaction at the major groove of DNA spiral.

Use the Watson-Crick basepairing rule, can derive the anti sense nucleotide sequence that is suitable for reducing this protein active from the nucleotide sequence (for example nucleotide sequence shown in sequence chart 1a, 1b, 1c or the 1d, or its homologue, analogue, collateral line homologue, lineal homologue) of coded protein.Anti sense nucleotide sequence can with described proteinic all transcript mRNA complementations, it can be limited to the coding region or can only be made of the part complementation of the coding of described oligonucleotide and mRNA or non-coding sequence an oligonucleotide.Therefore, oligonucleotide can be for example be used for the initial nucleic acid district complementation of described protein translation with comprising.Anti sense nucleotide sequence length can be favourable be for example 5,10,15,20,25,30,35,40,45 or 50 Nucleotide, but also can be longer and comprise at least 100,200,500,1000,2000 or 5000 Nucleotide.The known method of anti sense nucleotide sequence available techniques personnel is carried out recombinant expressed or is synthesized by chemistry or enzyme process.About chemosynthesis, available natural or modified Nucleotide.Modified Nucleotide can strengthen the stable physical stability that also can strengthen anti sense nucleotide sequence and the duplex of adopted target sequence formation is arranged of biological chemistry of anti sense nucleotide sequence.Available Nucleotide is, thiophosphoric acid derivative and for example through acridine substituted nucleotide, as 5 FU 5 fluorouracil, 5-bromouracil, the 5-chlorouracil, 5-iodouracil, xanthoglobulin, xanthine, the 4-acetylcytosine, 5-(carboxyl hydroxymethyl) uridylic, 5-carboxyl methylamino methyl-2-sulphur uridine, 5-carboxyl methylamino 6-Methyl Uracil, two hydrogen uridylics, β-D-galactosyl queosine, Trophicardyl, the N6-isopentennyladenine, the 1-methyl guanine, the 1-methyl inosine, 2,2-monomethyl guanine, the 2-methyladenine, the 2-methyl guanine, the 3-methylcystein, 5-methylcytosine, the N6-VITAMIN B4, the 7-methyl guanine, 5-methylamino 6-Methyl Uracil, 5-methoxyl group amino methyl-2-thiouracil, β-D-mannose group queosine, 5-methoxyl group carboxyl 6-Methyl Uracil, the 5-methoxyuracil, 2-methylthio group-N6-isopentennyladenine, uridylic-5-ethoxyacetic acid, pseudouracil, queosine, 2-sulfo-cytosine(Cyt), 5-methyl-2-deracil, the 2-deracil, the 4-deracil, methyl uracil, uridylic-5-oxy acetic acid methyl ester, uridylic-5-fluoroacetic acid, 5-methyl-2-deracil, 3-(3-amino-3-N-2-carboxyl propyl group) uridylic and 2,6-diaminopurine.

In another embodiment preferred, expression by sequence chart 1a, 1b, 1c or 1d or one of its homologue, analogue, collateral line homologue and lineal homologue sequence encoded protein matter can suppress by such nucleotide sequence, so that reduce gene transcription, described nucleotide sequence and generegulation district (for example promotor and/or enhanser) complementation and with wherein dna double spiralization triple-helix structure.These class methods have (Helene C (1991) the Anticancer Drug Res 6 (6): 569-84 that describes; (1992) Ann NY Acad Sci 660:27-36 such as Helene C; Maher LJ (1992) Bioassays 14 (12): 807-815).

In another embodiment, antisense nucleic acid molecule can be α-end group isomery nucleic acid.This kind α-end group isomery nucleic acid molecule and complementary RNA form special double-stranded crossbred, and be opposite with conventional β-nucleic acid, two chains wherein ((1987) Nucleic Acids Res 15:6625-6641 such as Gautier C) parallel to each other.Antisense nucleic acid molecule also can further comprise 2 '-O-methyl ribonucleotides (Inoue etc. (1987) Nucleic Acids Res 15:6131-6148) or chimeric RNA-DNA analogue (Inoue etc. (1987) FEBS Lett 215:327-330).

Antisense nucleic acid molecule of the present invention introduces cell usually or original position produces, they have the cell mRNA and/or the genomic dna hybridization of the bioactive polypeptide of protein of the present invention with coding or combine thus, thereby the expression of arrestin matter is for example by suppressing to transcribe and/or to translate and to cause aforesaid compound X to improve active.

Antisense molecule of the present invention also comprises such nucleic acid molecule, and it contains the nucleotide sequence with the regulatory region (for example its promotor and/or enhanser) complementary (for example forming the triple-helix structure that stops genetic transcription in the target cell) of the natural nucleotide sequence that has a polypeptide (for example peptide sequence of identifying shown in the sequence list or according to methods described herein) of code book invention.Generally see Helene C (1991) Anticancer Drug Res 6 (6): 569-84; (1992) Ann NY Acad Sci660:27-36 such as Helene C; Maher LJ (1992) Bioassays 14 (12): 807-815.

C) introduce the anti sense nucleotide sequence that makes up with ribozyme

Antisense strategy described above can make up with the ribozyme method valuably.Catalytic RNA molecule or ribozyme can be suitable for any target RNA and cut phosphodiester backbone, the following functional inactivation target DNA (Tanner NK (1999) FEMS Microbiol Rev 23 (3): 257-275) at specific site.Therefore ribozyme self is not modified, but can further cut target RNA molecule in a similar fashion, so it has obtained the characteristic of enzyme.Ribozyme sequence is incorporated into to be given these sense-rna enzyme samples RNA cutting characteristic and has therefore strengthened their efficient in inactivation target RNA in the sense-rna.The preparation of suitable ribozyme antisense rna molecule and application are described in for example (1988) Nature 334:585-591 such as Haseloff.

By this way, ribozyme (hammerhead ribozyme for example; Haselhoff and Gerlach (1988) Nature 334:585-591) mRNA and the prevention that can be used for the enzyme of catalytic cutting desire inhibition translated.The ribozyme technology can strengthen the efficient of antisense strategy.The method that expression is used for reducing some proteinic ribozyme is described at (EP 0 291 533, EP 0 321 201, EP 0 360 257).Ribozyme expression in vegetable cell is also described ((1992) EMBO J 11 (4): 1525-1530 such as Steinecke P to some extent; (1996) Mol Gen Genet.250 (3) such as de Feyter R: 329-338).Suitable target sequence and ribozyme can for example pass through " Steinecke P; " Ribozymes; Methods inCell Biology 50 "; volumes such as Galbraith; Academic press, Inc (1995), 449-460 page or leaf " description, calculate the secondary structure of ribozyme rna and target RNA, and by their interaction ((1992) Plant Mol Biol.18 (2): 353-361 such as Bayley CC; (1994) Mol Gen Genet.242 (6) such as Lloyd AM and Davis RW: 653-657) determine.For example, can make up thermophilas L-19IVS RNA derivative, described thermophilas L-19IVS RNA derivative has the complementation district of the protein mRNA that desires to suppress.(also referring to US 4,987,071 and US 5,116,742).Alternatively, the also available screening method of this type of ribozyme is identified (Bartel D and Szostak JW (1993) Science 261:1411-1418) from various ribozymes library.

D) introduce (justice is arranged) nucleotide sequence that is used to induce common inhibition

The expression of nucleotide sequence sense orientation can cause the common inhibition of corresponding homology native gene.Have the expression that adopted RNA is arranged of homology can reduce or eliminate in fact the expression of native gene with native gene, this mode of action is similar to antisense method described below: Jorgensen etc. (1996) Plant MolBiol 31 (5): 957-973; Goring etc. (1991) Proc Natl Acad Sci USA 88:1770-1774; Smith etc. (1990) Mol Gen Genet 224:447-481; Napoli etc. (1990) Plant Cell 2:279-289; (1990) Plant Cell 2:291-99 such as Van der Krol.In this article, the construct of importing can be fully or the part representative homologous genes desiring to reduce only.This kind technical description that is applied to plant is in (1990) The Plant Cell 2:279-289 such as for example Napoli and US 5,034,323.

E) introduce coding dominant negative regulation nucleic acid sequences to proteins

Also can effectively reduce proteinic function or activity by expressing described proteinic dominant negative regulation variant.Those skilled in the art are familiar with reducing protein function or active method (Lagna G and Hemmati-Brivanlou A (1998) Cur-rent Topics in Developmental Biology 36:75-98 by the method for its dominant negative regulation form of coexpression; Perlmutter RM and Alberola-lla J (1996) Current Opinion in Immunology 8 (2): 285-90; Sheppard D (1994) American Journal of Respiratory Cell﹠amp; MolecularBiology11 (1): 1-6; Herskowitz I (1987) Nature 329 (6136): 219-22).

Can produce the dominant negative regulation variant by for example in one of sequence chart 1a, 1b, 1c or 1d sequence or its homologue encoded protein matter, changing amino acid.This change can be decided by for example computer assisted comparison (" comparison ").Preferably on the nucleotide sequence level, produce the sudden change of dominant negative regulation variant.The vitro mutagenesis that can mediate by the PCR that for example uses suitable Oligonucleolide primers suddenlys change accordingly, introduces the sudden change that needs by described PCR.For this reason, the method for using those skilled in the art to be familiar with.For example, " LA PCR vitro mutagenesis test kit " (Takara Shuzo Kyoto) can be used for this purpose.As well known to those skilled in the art by deletion change functional structure territory (for example TF) or other can in conjunction with but not the activated sets of signals assign to reduce protein active.

F) introduce at gene, RNA or protein DNA binding factor or the protein bound factor

Also can reduce one of the sequence of sequence chart 1a of the present invention, 1b, 1c or 1d or the expression of gene of its homologue coding with specific DNA binding factor (for example using the factor of zinc finger transcription factor type).The genome sequence of these factors and endogenous target gene (sequence of preferred regulatory region) is annealed, and causes the inhibition of native gene.The expression that can reduce native gene that the application of this kind method makes without the reorganization operation.These class methods of preparation correlation factor are described in (2001) J BiolChem 276 (31): 29466-78 such as Dreier B; (2000) J Mol Biol 303 (4): 489-502 such as Dreier B; (2000) Proc Natl Acad Sci USA 97 (4): 1495-1500 such as Beerli RR; (2000) J Biol Chem 275 (42): 32617-32627 such as Beerli RR; Segal DJ and Barbas CF 3rd (2000) Curr Opin Chem Biol 4 (1): 34-39; Kang JS and Kim JS (2000) J Biol Chem 275 (12): 8742-8748; (1998) Proc Natl Acad SciUSA 95 (25): 14628-14633 such as Beerli RR; (1997) Proc Natl Acad Sci USA94 (8): 3616-3620 such as Kim JS; Klug A (1999) J Mol Biol 293 (2) 215-218; (1998) Adv Drug Deliv Rev 30 (1-3): 23-31 such as Tsai SY; (2000) Proc Natl Acad Sci USA 97 (8): 3930-3935 such as Mapp AK; (1997) IntJ Biochem Cell Biol 29 (12) 1371-1387 such as Sharrocks AD; (2000) J Biol Chem275 (43): 33850-33860 such as Zhang L.The example that this technology is used in plant is at WO 01/52620, (Proc.Natl.Acad.Sci.USA such as OrdizMI, Vol.99, Issue 20,13290-13295,2002) or Guar etc., (Issue 20 for Proc.Natl.Acad.Sci.USA, Vol.99,13296-13301,2002) describe to some extent in.

This type of factor can be selected with any part of gene.Preferably, this fragment is positioned at promoter region.Yet,, also can be positioned at the zone of coding exon or intron for gene inhibition.From Genbank, obtain relevant fragment or start from the non-existent cDNA of gene among the Genbank by database search for the technician, obtain by screen corresponding genomic clone from genomic library.

Can also at first in the target crop, identify sequence, find promotor then and use the above-mentioned factor to reduce expression by one of sequence chart 1a, 1b, 1c or 1d sequence or its homologue coding.

The technician is familiar with required for this reason method.

In addition, the factor of transfered cell also himself can suppress those of target protein.The protein bound factor can be, for example fit (Famulok M and Mayer G (1999) Curr TopMicrobiol Immunol 243:123-36) or antigen or antibody fragment or single-chain antibody.Obtain that the mode of these factors is existing to be described and be that the technician is known.For example, utilize tenuigenin scFv antibody in genetically modified tobacco plant, to regulate plant pigments A activity of proteins ((1992) Biotechnology (N Y) 10 (7): 790-794 such as Owen M; (1997) Curr Opin Biotechnol 8 (4): 411-416 such as Franken E; Whitelam (1996) Trend Plant Sci1:286-272).

Genetic expression also can suppress (Dervan PB and B ü rli RW (1999) Current Opinion in Chemical Biology3:688-693 by suitable lower molecular weight synthetic compound (for example polyamide-based); (2000) Gene Expr 9 (1-2) such as Gottesfeld JM: 77-91).These oligomers are by 3-(dimethylamino) propylamine, N-methyl-3-hydroxyl pyrroles, N-Methylimidazole and the N-methylpyrrole is formed and with their sequence-specific combinations and suppress to be positioned at any part that mode that gene order wherein expresses can be suitable for double-stranded DNA in major groove.Appropriate means has been described in (2001) Bioorg Med Chem.9 (8): 2093-103 such as Bremer RE; (2001) Chem Biol.8 (6): 583-92 such as Ansari AZ; Gottesfeld JM etc. (2001) J Mol Biol.309 (3): (2001) Org Lett 3 (8) 1201-3 such as 615-29:Wurtz NR; (2001) Bioorg Med Chem 9 (3): 653-7 such as Wang CC; Urbach AR and Dervan PB (2001) Proc Natl Acad Sci USA 98 (8): 4343-8; (2000) J Biol Chem.275 (32): 24246-54 such as Chiang SY.

G) cause nucleic acid sequence and the expression construct that RNA degrades

Also can be by means of virus expression systems (amplicon), (Angell, SM etc. (1999) Plant is (3) J.20: 357-362) by inducing the special RNA of biology (being preferably plant) degraded effectively to produce inactivation or downward modulation.By means of virus vector, will import in the plant with the transcript homologous nucleotide sequence of desiring to suppress by this type systematic that is also referred to as " VIGS " (gene silencing of virus induction).May stop by the defense mechanism of plant virus resistance transcribing then.Suitable technology and method are described in (2001) Plant J 25 (2): 237-45 such as Ratcliff F; Fagard M and Vaucheret H (2000) Plant Mol Biol 43 (2-3): 285-93; (1998) Proc Natl Acad Sci USA 95 (22): 13079-84 such as Anandalakshmi R; Ruiz MT (1998) Plant Cell10 (6): 937-46.

H) importing is used to induce the construct of the homologous recombination (for example be used for producing and knock out mutant) of native gene

For generation has the active homologous recombination organism of reduction, use for example to comprise to the nucleic acid construct of small part native gene, described native gene is modified by lacking, add or substitute at least one Nucleotide with functional reduction or complete invalid mode.Modify the regulatory element (for example promotor) that also can influence gene and remain unchanged, but do not express (transcribe and/or translate) or express reduction down to encoding sequence.

About the homologous recombination of routine, 5 ' and 3 ' the distolateral wing in modified district is other nucleotide sequence that length is enough to be used in recombinating.In principle, length is at a hundreds of base (Thomas KR and Capecchi MR (1987) Cell 51:503 to the scope of several thousand bases; Strepp etc. (1998) Proc Natl Acad Sci USA 95 (8): 4368-4373).For homologous recombination, transform host organisms (for example plant) with the method for hereinafter describing with recombinant precursor, and select the successfully clone of reorganization with for example microbiotic or herbicide resistance.Use the cotransformation technology, can be then by hybridizing favourable resistance of removing again to microbiotic or herbicide.The example of efficient homologous recombination system is at Nat.Biotechnol.2002Oct in the plant; 20 (10): 1030-4, Terada R etc.: open among the Efficient genetargeting by homologous recombination in rice.

Homologous recombination is rare relatively event in higher eucaryote (particularly plant).Random integration accounts for main aspect in host genome.Removal through the random integration sequence and therefore the possibility of the correct homologous recombination cell clone of enrichment be as US 6,110, that describes in 736 uses the sequence specific recombination system, can lack non-special integration sequence by described homologous recombination system, this helps to select the incident successfully integrated by homologous recombination.Can use multiple sequence specific recombination system, the example that can mention is the Gin recombinase of Cre/lox system, zymic FLP/FRT system, the phage Mu of phage P1, from the R/RS system of Pin recombinase and the plasmid pSR1 of intestinal bacteria (E.coli).Preferably phage P1 Cre/lox and yeast FLP/FRT system.FLP/FRT and cre/lox recombinase system are used (Odell etc. (1990) MolGen Genet 223:369-378) in botanical system.

I) be used to produce the sudden change (for example producing termination codon, reading frame shift or the like) that function is lost to the native gene introducing

Be used for reducing active other proper method for nonsense mutation is imported to native gene, for example by the RNA/DNA oligonucleotide is imported in the plant (Zhu etc. (2000) Nat Biotechnol18 (5): 555-558) and for example by means of T-DNA mutagenesis (Koncz etc. (1992) Plant MolBiol 20 (5): 963-976) and ENU (N-ethyl-N-nitrosourea) mutagenesis or homologous recombination (HohnB and Puchta (1999) H Proc Natl Acad Sci USA 96:8321-8323) produce and knock out mutant.Point mutation also can be passed through DNA RNA hybrid (Cole-Strauss etc. (1999) NuclAcids Res 27 (5): 1323-1330; Kmiec (1999) Gene Therapy AmericanScientist 87 (3): 240-247) produce, described DNA RNA hybrid also is known as chimeric prosthesis (chimeraplasty).The mutational site can be selectively targeted or select at random.

As B) to I) described in nucleotide sequence express in cell or biology by cell or biological conversion/transfection, perhaps for example as A) disclosed, in currently known methods introducing cell or organism.

Being used for reducing active other proper methods is at the nucleic acid of vegetable cell introducing with one or more nucleic acid sequence encoding gene interactions of the sequence that is selected from sequence chart 1a, 1b, 1c or 1d and/or its homologue.But the nucleic acid of the autoactivation of introducing and the interaction of gene cause the inactivation of described gene at last by disappearance, inversion or insertion (promptly by reading frame shift or destruction).

Particularly, the invention provides the method that produces plant transformed with the nucleic acid of encoding gene, wherein the inactivation of gene or downward modulation described in this plant cause comparing the patience that environment-stress improves with corresponding unconverted wild-type plant cell, the raising of patience preferably realizes that by changing metabolic activity described method comprises by the nucleotide sequence of mutant nucleotide sequence Fig. 1 a, 1b, 1c or 1d or its homologue comes inactivation or downward modulation.

Binary vector (as pBinAR) can be used for this type of Plant Transformation (Hofgen and Willmitzer, 1990Plant Science 66:221-230).Other suitable binary vectors for for example pBIN19, pBI101, pGPTV or pPZP (Hajukiewicz, P etc., 1994, Plant Mol.Biol., 25:989-994).The summary of binary vector and characteristic thereof is seen Hellens etc., 2000, and Trends in plantscience, 5:446-451.

Can be by T-DNA makes up binary plasmid by having justice or antisense orientation to connect into cDNA.Plant promoter activates transcribing of cDNA at cDNA 5 '.The polyadenylation sequence is positioned at cDNA 3 '.Can realize tissue specific expression by using tissue-specific promoter listed above.Also can use any other promoter element.For the constitutive expression in the whole plants, can use the CaMV35S promotor.Can use signal peptide with expressed protein lead cellular compartment, for example plastid, plastosome or endoplasmic reticulum (Kermode, 1996Crit.Rev.Plant Sci.4 (15): 285-423).Be cloned into cDNA 5 ' in the signal peptide frame, to realize the Subcellular Localization of fusion rotein.In addition, can use the promotor that reacts on abiotic stress, as the sub-RD29A of arabidopsis thaliana promoter.The promotor that those of skill in the art will recognize that use should effectively be connected with nucleic acid, thereby promotor causes transcribing of nucleic acid, thereby causes the mRNA of composite coding polypeptide.In addition, the RNA that is used to influence same or other genetic expressions can be a sense-rna.

The alternative method of transfection comprises by electroporation or agriculture bacillus mediated transgenosis to be transferred to dna direct in the spending of growth.Can use the agrobacterium tumefaciens bacterial strain for example GV3101 (pMP90) (Koncz and Schell, 1986Mol.Gen.Genet.204:383-396) or LBA4404 (Ooms etc., Plasmid, 1982,7:15-29; Hoekema etc., Nature, 1983,303:179-180) implement agriculture bacillus mediated Plant Transformation.Can transform (Deblaere etc., 1994Nucl.Acids.Res.13:4777-4788 by standard conversion and regeneration techniques; Gelvin and Schilperoort, " PlantMolecular Biology Manual " second edition, Dordrecht:Kluwer Academic Publ., 1995.-in Sect., Ringbuc Zentrale Signatur:BT11-P ISBN 0-7923-2731-4; Glick, B R and Thompson, J E, " Methods in Plant Molecular Biology andBiotechnology ", Boca Raton:CRC Press, 1993.-360S., ISBN 0-8493-5164-2).For example can be converted and transform Semen Brassicae campestris (Moloney etc., 1989Plant Cell Reports 8:238-242 by cotyledon or plumular axis; De Block etc., 1989 Plant Physio.91:694-701).Binary vector and the agrobacterium strains that is used to transform depended in the selection of antibiotic use of Agrobacterium and plant.The selection of Semen Brassicae campestris uses kantlex as selectable plant mark usually.The agriculture bacillus mediated transgenosis to flax can be used for example Mlynarova etc., and the described technology of 1994 PlantCell Report 13:282-285 is carried out.In addition, the conversion of soybean can be used for example European Patent No.0424047, U.S.Patent No.5,322,783, EuropeanPatent No.0397687, U.S.Patent No.5,376,543 or U.S.Patent No.5,169,770 described technology are carried out.The conversion of corn can realize (consulting for example Freeling and Walbot " The maizehandbook ", Springer Verlag:New York (1993) ISBN 3-540-97826-7) by the DNA picked-up of particle bombardment, polyoxyethylene glycol mediation or by the silicon carbide technology.The specific examples that corn transforms is found in the U.S. patent No. 5,990,387, and the specific examples that wheat transforms is found in PCT Application No.WO 93/07256.

The useful method of determining gene transcription level (can be used for translating into the pointer of the mRNA amount of gene product) is to carry out Northern trace (reference example such as Ausubel etc., 1988 " CurrentProtocols in Molecular Biology ", Wiley:New York).This information to small part has illustrated the gene transcription degree.Can be by Several Methods known in the art (for example Bormann, E.R. etc. are described in the 1992Mol.Microbiol.6:317-326) total cell RNA of preparation from cell, tissue or organ.For proteinic existence or the relative quantity of assessment, can use standard technique (as the Western trace) from this mRNA translation.These technology are (consulting for example Ausubel etc., 1988 " Current Protocols in Molecular Biology ", Wiley:New York) well known to those of ordinary skill in the art.Also can use Real Time PCR.

The present invention in addition can also with contain the isolating recombinant expression vector combination of coercing the related protein coding nucleic acid, wherein carrier or coerce the related protein coding nucleic acid respectively the expression in host cell cause comparing patience and/or the resistance that environment-stress improves with the wild-type host cell, described change preferably realizes by changing metabolic activity.Term used herein " carrier " refers to transport the nucleic acid molecule of other coupled nucleic acid molecule.One type carrier is " plasmid ", refers to connect therein the into circular double-stranded DNA ring of extra dna fragmentation.The carrier of another type is a virus vector, wherein extra dna fragmentation can be connected in the viral genome.Some carrier can be in the host cell of its introducing self-replicating (bacteria carrier and the episome Mammals carrier that for example have the bacterium replication origin).Other carriers (for example non-add body Mammals carrier) are integrated into the host cell gene group after introducing host cell, thereby can duplicate with host genome.In addition, some carrier can instruct and its expression of gene that effectively is connected.These carriers are referred to herein as " expression vector ".Generally speaking, the expression vector that uses in the recombinant DNA technology often is the plasmid form.Because plasmid is the carrier format that the most generally uses, therefore " plasmid " and " carrier " can exchange use in this manual.Yet the present invention is intended to the other forms of expression vector that comprises that these provide identical function, as virus vector (for example duplicating the deficient retrovirus).

Also comprise the expression of plants box that contains nucleic acid construct in the scope of the present invention, described nucleic acid construct can or be reduced by the sequence that is selected from sequence chart 1a, 1b, 1c or 1d and/or its homologue and/or the coded gene of its part by aforesaid method inactivation when expressing, thereby cause the patience and/or the resistance that improve, described raising preferably realizes by changing metabolic activity.

The expression of plants box preferably contains and can drive genetic expression and make each sequence can carry out the adjusting sequence of its function Transcription Termination of polyadenylation signal (for example by) by effective connection in vegetable cell.Preferred polyadenylation signal is for deriving from agrobacterium tumefaciens T-DNA, for example being known as is the gene 3 (Gielen etc. of Ti-plasmid pTiACH5 octopine synthase, 1984, EMBO is J.3:835) or its functional equivalent, but every other the active terminator of function is arranged in plant also all is suitable.

The expression of plant gene must be connected with suitable promotor in good time, that the specific mode of cell or tissue is expressed with making gene.Preferred promotor (the Benfey etc. that drive constitutive expression, 1989EMBO is J.8:2195-2202) as promotor from plant virus, as 35S CaMV (Franck etc., 1980 Cell 21:285-294), 19S CaMV (consulting U.S.Patent No.5352605 and PCT Application No.WO 8402913) or plant promoter, as U.S.Patent No.4, the promotor of the ribulose diphosphate carboxylation-oxygenase small subunit described in 962,028.

Other favourable adjusting sequences comprise for example plant promoter, as CaMV/35S (Franck etc., Cell 21 (1980) 285-294), PRP1 (Ward etc., Plant.Mol.Biol.22 (1993)), SSU, OCS, lib4, usp, STLS1, B33, LEB4, no or ubiquitin, rapeseed protein or phaseolin promoter.Useful in addition plant promoter is potato kytoplasm FBPase promotor or ST-LS1 promotor (Stockhaus etc., EMBO J.8,1989,2445), the noden specificity promoter of describing among soybean phosphorus ribose tetra-sodium transaminase promotor (gene bank accession number U87999) or the EP-A-0249676.Other particularly advantageous promotors are for being used for the seed specific promoters of monocotyledons or dicotyledons, be described in US 5,608,152 (the rapeseed protein promotors in Semen Brassicae campestris source), WO 98/45461 (phaseolin promoter in Arabidopis thaliana source), US 5,504,200 (phaseolin promoters in Kidney bean source), WO 91/13980 (the Bce4 promotor in rape source) and Baeumlein etc., Plant J., 2,2,1992:233-239 (the LEB4 promotor in pulse family source).Described promotor can be used for dicotyledons.Below be used for monocotyledonous promotor and be the Ipt-2 in for example barley source or the barley protein promoter in Ipt-1 promotor (WO 95/15389 and WO95/23230) or barley source.Other useful promotors are described in WO99/16890.

In principle can with aforementioned novel method in mention those equally use all natural promoters and regulate sequences and carry out inactivation or downward modulation, to reduce the level that target protein produces.

Gene construct can also contain and is useful on other genes that insert biological and for example relevant with stress resistance (promptly near some inactivation gene or at the gene of its site integration inactivation), can introduce and the relevant beneficial gene of proteinic generation that can actively improve stress tolerance or resistance.Therefore in host living beings, insert and express regulatory gene be possible and favourable, regulatory gene for elicitor for example, prevent son or get involved the gene of the enzyme of regulating, or one or more in the full gene of biosynthetic pathway by its enzymic activity.These genes can be allos or homologous on the source.The gene that inserts can have the promotor of himself or place under the control of same promotor with nucleotide sequence or its homologue of sequence chart 1a, 1b, 1c or 1d.

In order to express other genes of existence, 3 ' and/or 5 ' the extra end that contains that gene construct is favourable is regulated sequence, expresses to strengthen.Depend on selected host living beings and gene, for optimum expression selects to regulate sequence.

These are regulated sequence and are intended to realize specific gene expression and protein expression as the aforementioned.Depend on host living beings, this can refer to that for example gene is only expressed or crossed and express after inducing, or expresses and/or cross expression immediately.

Preferably regulate sequence or factor pair introducing expression of gene in addition and have favorable influence, and thereby improve and express.Can transcribe signal (as promotor and/or enhanser) by force at the favourable enhancing regulatory element of transcriptional level by using by this way.Yet, also can strengthen translation in addition by the stability that for example improves mRNA.

Other preferred sequence of using in the gene expression in plants box (can be consulted Kermode as the guiding gene product to the necessary target sequence of its suitable cellular compartment, 1996 Crit.Rev.Plant Sci.15 (4): the summary of 285-423 and the reference of quoting thereof), other compartments in described compartment such as vacuole, nucleus, all types of plastid (as amyloplast, chloroplast(id), chromoplast), born of the same parents' external space, plastosome, endoplasmic reticulum, oil body, peroxysome and the vegetable cell.

Table 1: the example of tissue specificity and stress induced promoter in the plant

Express	Reference
		Cor78-is cold, arid, salt, ABA, wound-induced	Ishitani etc., Plant Cell 9:1935-1949 (1997). Yamaguchi-Shinozaki and Shinozaki, Plant Cell 6:251-264 (1994)
Rci2A-is cold, dehydration is induced	Plant Physiol 115:569-576 (1997) such as Capel
		Rd22-arid, salt	Yamaguchi-Shinozaki and Shinozaki, Mol Gen Genet 238:17-25 (1993)
Cor15A-is cold, dehydration, ABA	Baker etc., Plant Mol.Biol.24:701-713 (1994)
		The GH3-growth hormone induction	Liu etc., Plant Cell 6:645-657 (1994)
The ARSK1-root, salt is induced	Hwan and Goodman, Plant J 8:37-43 (1995).
		The PtxA-root, salt is induced	GenBank accession number X67427
The SbHRGP3-Gent is different	Ahn etc., Plant Cell 8:1477-1490 (1998)
		KST1-guard cell's specificity	Plesch etc., Plant Journal.28 (4): 455-64, (2001)
KAT1-guard cell's specificity	Plesch etc., Gene 249:83-89 (2000) Nakamura etc., Plant Physiol.109:371-374 (1995)
		Induced by Salicylic Acid	PCT Application No.WO 95/19443
Tsiklomitsin is induced	Plant such as Gatz are (1992) J.2:397-404
		Alcohol induced	PCT Application No.WO 93/21334
The PRP1 of pathogen-inducible	Ward etc., 1993 Plant.Mol.Biol.22:361-366
		Thermoinducible hsp80	U.S.Patent No.5187267
Cold inductive αDian Fenmei	PCT Application No.WO 96/12814
		The pinII of wound-induced	European Patent No.375091
RD29A-salt is induced	Yamaguchi-Shinozalei etc., (1993) Mol.Gen. Genet.236:331-340
		Plastid specificity virus RNA polymerase	Application No.WO 95/16783 and WO 97/06250

The for example super promotor of other selecting and labelling systems such as AHAS mark or other promotors (literary composition sees before), ubiquitin promoter (Callis etc., J.Biol.Chem., 1990,265:12486-12493; US5,510,474; US 6,020, and 190; Kawalleck etc., Plant.Molecular Biology, 1993,21:673-684) or 10S promotor (GenBank accession number M59930 and X16673) also can similarly be used for of the present invention group merge into conventionally known to one of skill in the art.

Particularly, the invention describes use obtains stress tolerance and/or resistance (i.e. arid, salt and/or cold patience and/or resistance) by the metabolic activity of inactivation or down-regulated gene change plant.This paper has illustrated the strategy of Arabidopis thaliana, but its application is not limited only to these plants.Therefore the invention provides and contain one or more nucleic acid or (the coercing the related protein coding) gene of its homologue and transgenic plant of stress tolerance and/or resistance that are selected from sequence chart 1a, 1b, 1c or 1d, described patience and/or resistance preferably realize by changing metabolic activity, wherein (environment) coerced and is arid, high salt or high temperature or low temperature, but its application is not limited to these adverse environments.For example can obtain protection to other unfavourable condition (as heat, atmospheric pollution, heavy metal and chemical toxicant).In preferred embodiments, environment-stress is an arid.

Plant that growth is modified under stress conditions and screening and analyze growth characteristics and/or metabolic activity, with genetic modification in the assessment plant to the influence of stress tolerance and/or resistance.These analytical technologies are well known to those skilled in the art.They comprise screening (Rompp Lexikon Biotechnologie, Stuttgart/New York:Georg Thieme Verlag 1992, " screening " 701 pages) dry weight, weight in wet base, protein synthesis, carbohydrate is synthetic, lipid is synthetic, water-use ratio, common plant and/or crop yield, bloom, breed, set seeds, root growth, respiration rate, photosynthetic rate etc.(" Applications of HPLC in Biochemistry in:Laboratory Techniques inBiochemistry and Molecular Biology ", the 17th volume; Rehm etc., 1993 " Biotechnology ", the 3rd volume, III chapter: " Product recovery and purification ", 469-714 page or leaf, VCH:Weinheim; Belter, P.A. etc., 1988, " Bioseparations:downstream processing for biotechnology ", John Wiley and Sons; Kennedy, J.F. and Cabral, J.M.S., 1992, " Recovery processes for biologicalmaterials ", John Wiley and Sons; Shaeiwitz, J.A. and Henry, J.D., 1988, " Biochemical separations, in:Ulmann ' s Encyclopedia of IndustrialChemistry ", B3 volume, Chapter 11,1-27 page or leaf, VCH:Weinheim; And Dechow, F.J., 1989, " Separation and purification techniques in biotechnology ", NoyesPublications).

Also can use method of the present invention, the metabolic activity of comparing change with non-stress conditions by the screening vegetable cell detects the environment-stress in vegetable cell or the plant, and described screening allows to select resistance or patience plant or vegetable cell also providing the detection of coercing in plant or the vegetable cell before visible symptom and havoc.

Method of the present invention also allows to cultivate environment-stress is improved patience and/or the vegetable cell of resistance or the method for plant, and described method comprises that screening compares the metabolic activity that vegetable cell changes under the stress conditions and select and environment-stress is had those that improve patience and/or resistance further repeat with non-stress conditions.

Also may obtain having to one or several operations of coercing the associated protein plasmagene of the present invention change active and remote effect plant coerce reaction and/or stress tolerance coerce the associated protein plasmagene.For example, metabolic normal biochemical process causes the multiple generation that can actively influence the product (for example hydrogen peroxide and other reactive oxygen specieses) of same metabolic process.For example, known peroxynitrite can nitrated tyrosine side chain, thereby makes some enzyme deactivation (Groves, J.T., 1999, the Curr.Opin.Chem.Biol.3 (2): 226-235) that contains tyrosine at avtive spot.Coerce the inactivation of related protein or high stress tolerance and/or the resistance that downward modulation may cause cell by optimizing one or more the present invention.

What in addition, sequence disclosed herein or its fragment can be used for producing multiple biology (as bacterium, mammalian cell, yeast cell and vegetable cell) genome knocks out sudden change (Girke, T., 1998, The Plant Journal 15:39-48).Can assess the ability that knocks out the multiple stress conditions of cell tolerance of generation or capacity then, it is to the reaction of multiple stress conditions and the phenotype and/or the genotypic influence of sudden change.The method of other gene inactivations is consulted U.S.Patent No.6,004,804 " Non-ChimericMutational Vectors " and Puttaraju etc., 1999, Spliceosome-mediated RNAtrans-splicing as a tool for gene therapy, Nature Biotechnology 17:246-252.

Quoted multiple publication in the application's full text.The reference of quoting in the disclosure of all these publications and these publications is introduced among the application as a reference in full with it, with the situation in field under more detailed description the present invention.

The present invention is not limited to concrete nucleic acid, polypeptide, cell type, host cell, conditioned disjunction method of indication etc., but can change to some extent, and those skilled in the art know a large amount of modifications and change.Will also be understood that term used herein only is used to describe specific embodiments, and unconfined intention.

Should also be appreciated that preamble relates to the preferred embodiment of the invention, can produce numerous variations and change without departing from the present invention.The present invention further specifies by following examples, and described embodiment should not be interpreted as restrictive by any way.On the contrary, should be clearly understood, after the description of reading this paper, can under the situation of the scope that does not break away from spirit of the present invention and/or claims, point out multiple other embodiments of those skilled in the art, change and its equivalents.

Embodiment

Coerce the anti-arabidopsis thaliana of coercing that the genes involved acquisition has the metabolic activity of change by inactivation or downward modulation

The conversion of Arabidopis thaliana

Preparing carriers

(contain and be useful on the kanamycin gene Hajukiewicz that bacterium is selected, P. etc., 1994 based on the pPZP binary vector main chain of modifying, Plant Mol.Biol. is 25:989-994) with by mas2 ' 1 ' and mas271f promotor (Velten etc., 1984, EMBO J.3,2723-2730; Mengiste, Amedeo and Paszkowski, 1997, Plant J., 12,945-948) the selective marker bar gene of Qu Donging (De Block etc., 1987, J.6 EMBO 2513-2518) has made up binary vector.Complete carrier (Fig. 2) and plasmid have been shown in the annex.

Other binary vectors that can be used for inserting mutagenesis are pBIN19, pBI101, pBinAR or pGPTV.The summary of binary vector and characteristic thereof is seen Hellens etc., 2000, Trends in plantScience, 5:446-451 and GuerineauF., Munineaux P., 1993, Planttransformation and expression vectors in plant molecular biology, LABFAX Series, (Croy R.R.D. volume) 121-127 page or leaf Bios ScientificPublishers, Oxford.

Agrobacterium-mediated Transformation

Use heat shock or electroporation scheme that plasmid is transformed into agrobacterium tumefaciens (GV3101pMP90; Koncz and Schell, 1986, Mol.Gen.Genet.204:383-396).The bacterium colony that transforms is cultivated on the YEP substratum and is selected 2 days by microbiotic separately at 28 ℃.Carry out Plant Transformation with these Agrobacterium cultures.

According to standard conditions (Bechtold, N., Ellis, J., Pelletier, G.1993.In plantaAgrobacterinm mediated gene transfer by in filtration of Arabidopsisthaliana plants.R.Acad.Sci.Paris.316:1194-1199; Bent, A.F., Clough, J.C., 1998; Floral dip:a simplified method forAgrobacterium-mediated transformation of Arabidopsis thaliana, PLANT is J.16:735-743) cultivate and the environmental Arabidopis thaliana of conversion C24.

Use its resistant gene separately to select plant transformed (F1).For BASTA ^_Resistance, seedling is with 0.02% BASTA ^_Spray four times with 2 to 3 days intervals, plant transformed is set seeds.50-100 young plant (F2) is carried out mark once more select, for BASTA ^_Resistance is at the BASTA of continuous 4 days of seedling phase sprinkling 0.1% ^_The plant of selecting the monoclonal antibody site to separate (the resistance seedling is about 3: 1 than responsive seedling) is further analyzed.Three strain resistance seedlings (F2) in these strains system are set seeds and by making its seed (F3) contain selective reagents (BASTA ^_, 15mg/L grass ammonium phosphine (ammonium glufosinate), Pestanal, Riedelde Haen, Seelze, Germany) nutrient agar on external sprouting test its homozygosity.Demonstration is to think to isozygoty near the F2 strain of 100% resistance filial generation (F3), and is used for functional selection.

The measurement of stress resistance

The transgenic arabidopsis plant is cultivated respectively in the basin of containing 4: 1 in the York culturing room (YorkIndustriek lte GmbH, Mannheim, the Germany) soil of (volume/volume) and quartz sand mixture.With the seed sowed in the dark 4 ℃ preserve 3 days to induce sprouting.The type culture condition is: the photoperiod that illumination in 16 hours and 8 hours are dark, 20 ℃, 60% relative humidity and 150uE photon flux density.The seed of sowing is kept 3 days to induce sprouting in 4 ℃ in the dark.Apply arid to plant watering during ages until about 3 weeks every day by stopping to water.Simultaneously, reduce relative humidity to 20% with 10% increment every other day.After stopping to water about 12 days, most of plants demonstrate visible injury symptom (as wilting and leaf becomes brown), and the patience plant is accredited as the green of the full and color of visible for health.Plant and adjacent plant made comparisons in subsequent three days the symptom of arid damage is marked.。

Three experiments have then been carried out.In first experiment, tested the body one by one of each transformation plant.

In second experiment, be that the strain system of patience or resistance (promptly more of a specified duration than wild-type contrast existence) confirms screening to scoring in first experiment with same experimental arrangement.In this experiment as preceding cultivation and handled each patience or maximum 5 strain plants of resistant strain system.

In preceding two experiments, relatively weigh patience or resistance with adjacent wild-type plant.

In the 3rd experiment (table 2), as preceding cultivation with handled at least 15 of each patience strain of determining system (promptly in second experiment scoring for patience or resistance), be generally 20-25 repetition.In the 3rd experiment, be determined at the average and maximum arid survival fate in the visible dead back of wild-type contrast.Use Mini-PAM (Heinz Walz GmbH, Effeltrich, Germany) to coerce in addition and measure (table 3) with non-chlorophyll fluorescence of coercing plant.

In the 3rd experiment, after arid 12 days, the contrast in the test (unconverted Arabidopis thaliana) and most of transformation plant demonstrate the extreme symptom of as seen coercing, and comprise necrosis and necrocytosis.Some transform plant, and it has kept viability with remaining green demonstration by its full outward appearance.

The chlorophyll fluorescence of (in the non-darkness adaption plant) photosynthesis output is measured and has been confirmed that 14 days drought stress has suppressed photosynthesis fully in control plant, and in most cases transformation plant can be kept photosynthetic function (table 3) for more time.

The analysis of the stress tolerance strain system that selects

Since preliminary election single insertion site and the resistance marker that isozygotys strain system, monogenic destruction in the T-DNA integration process (or sudden change) expectation can cause the stress resistance phenotype.Select the strain system of the consistent phenotype of performance to carry out analysis of molecules.

Use standard program (Qiagen, Hilden, the column spinner or the AmershamBiosciences of Germany, Freiburg, the Nucleon Phytopure test kit of Germany) to derive from purified genomic dna the leaf texture that these strains are from about 100mg.Use the inserting side of two kinds of different methods amplification T-DNA.According to Spertini D, Baliveau C. and Bellemare G., 1999, Biotechniques, 27, the adaptor PCR method of 308-314 uses T-DNA Auele Specific Primer LB1 (5 '-TGA CGC CAT TTC GCC TTT TCA-3 ' SEQ ID XXX) to carry out the first round, LB2 (5 '-CAG AAA TGG ATA AAT AGC CTT GCT TCC-3 ' XXX) or RB4-2 (5 '-AGC TGG CGT AAT AGC GAA GAG-3 ' XXX) carries out second to take turns.Carried out in addition TAIL-PCR (Liu Y-G, Mitsukawa N, OosumiT and Whittier RF, 1995, Plant J.8,457-463). in this case as the T-DNA Auele Specific Primer be respectively applied to a left side or right margin be the LB1 (5 '-TGACGC CAT TTC GCC TTT TCA-3 ' SEQ ID XXX) of first round PCR or RB1-2 (5 '-CAACTT AAT CGC CTT GCA GCA CA-3 ' XXX), second LB2 that takes turns (5 '-CAGAAA TGG ATA AAT AGC CTT GCT TCC-3 ' SEQ ID XXX) or RB4-2 (5 '-AGC TGG CGT AAT AGC GAA GAG-3 ' SEQ ID XXX) and last LB3 that takes turns (5 '-CCA ATA CAT TAC ACT AGC ATC TG-3 ' SEQ IDXXX) or RB5 (5 '-AAT GCT AGA GCA GCT TGA-3 ' SEQ ID XXX).

On sepharose, identify correct PCR product and use post and standard program purifying (Qiagen, Hilden, Germany).The PCR product checks order with the other T-DNA Auele Specific Primer that is positioned at the corresponding border of primer of using near amplification.In the sequencing reaction for the PCR product that contains the left margin sequence use primer LBseq (5 '-CAA TAC ATT ACA CTAGCA TCTG-3 '; SEQ ID XXX), the sequence that contains right border sequence use primer RBseq (5 '-AGA GGC CCG CAC CGATCG-3; SEQ ID XXX).Use the blast algorithm arabidopsis gene group sequence that the sequence that obtains and Genbank provide is compared (Altschul etc., 1990.J Mol Biol, 215:403-410).

Table 4 has provided the details of the PCR product that is used for identified gene group site.The expectation size (base pair) of the PCR product that what indicate is the exploitation reading frame through note that identifies in the arabidopsis gene group, obtain, realize amplification the T-DNA border (LB: left margin, RB: right margin), in the method (explanation sees above) of the PCR product that obtains indicating, adaptor PCR separately Restriction Enzyme and the degenerated primer among the TAIL PCR.Common other known primers use degenerated primer ADP3 that provide near table 4 (5 '-WGTGNAGWANCANAGA-3 '; SEQ ID XXX), ADP6 (5 '-AGWGNAGWANCANAGA-3 '; SEQ ID XXX) and ADP8 (5 '-NTGCGASWGANWAGAA-3 '; SEC ID XXX).

The primer that uses the T-DNA Auele Specific Primer in each case and release from genes identified group site contrasts the insertion site of PCR to confirm to identify.Use the amplification proof of the expection size PCR product of these two kinds of primers to destroy the site that identifies in the insertion strain system by the T-DNA integration.

The metabolic analysis of transgenic plant

Use following experimental implementation to identify that the described metabolism in the transgenic plant changes:

A) cultivation of plant and processing

Plant is in cultivating for three weeks (literary composition sees before) with potting soil under the standard conditions in climatic chamber.Gather in the crops the first eight day to part plant stop to water (8 days handle).Gathered in the crops preceding four days, to another group plant stop to water (4 days handle).The plant of " control treatment " is normally watered in that whole growth is interim.The plant that adjacent cultivation is analyzed in same analytical sequence is to avoid environmental influence.

B) sampling of sample and storage

Sampling is carried out in climatic chamber.Cut green portion with scissors, weigh rapidly and put into extraction tube (extraction thimble) immediately with the liquid nitrogen precooling.Extract tube and pallet before extraction-80 ℃ of storages.

C) freeze-drying

Plant is at solvent contact or remove moisture by freeze-drying and forbade in the past melting or reach＞-40 ℃ temperature.

Specimen holder and extraction tube are put in the freeze dryer of precooling (40 ℃).The starting temperature of main dry phase is-35 ℃, and pressure is 0.120mbar.For drying process, parameter changes according to the pressure and temperature program, and final (after 12 hours) temperature is+30 ℃, and pressure is 0.001-0.004mbar.Close after vacuum pump and the refrigerator, system passes to exsiccant air or argon gas.

D) extract

The extraction tube that will have vegetable material after the freeze-drying is immediately transferred in the 5mL extraction chamber of ASE (the accelerated solvent extractor ASE200 that has solvent controller and automatic ASE software (DIONEX)).

With about 10mL methanol (80/20, volume/volume), extract polar material with T=70 ℃, p=140bar, 5 minutes leaving standstill in 1 minute of heating phase.With about 10mL methanol (40/60, volume/volume), extract lipid material with T=70 ℃, p=140bar, 5 minutes leaving standstill in 1 minute of heating phase.Collect two kinds of extracts at an extraction flask (centrifuge tube, 50mL have the film that screw socket covered and be used for ASE (DIONEX)).

Following internal standard substance is added in the extract: LC-standard substance, L-methionine(Met)-d3, Boc-Ala-Gly-Gly-Gly-OH, L-tryptophane-d5, arginine ¹³C ₆ ¹⁵N ₄, ubiquinone 1,2,4 and ribitol, L-glycine-2,2-d2, L-L-Ala-2,3,3,3-d4, α methyl glucopyranoside, Nonadecanoic acid methylester, undecanoic acid methyl esters, tridecanoic acid, pentadecylic acid, montanic acid.In the mixture that produces, add 8mL water.Abandon the solid residue of plant and extract tube.

Extract was separated with acceleration at the centrifugal 5-10 of 1400g minute.Respectively take out from colourless methanol upper strata (polarity) that 1mL is used for GC and LC analyzes mutually.Abandon remaining upper strata phase.Take out from bottle-green organic lower floor that 0.5mL is used for GC and LC analyzes mutually respectively.Use the infrared vacuum-evaporator of IR-Dancer (Hettich) all sample parts of evaporation under 40 ℃ of top temperatures and peak pressure 10mbar.

E) LC/MS and LC/MS/MS analyze

In lipid and polarity residue, add HPLC moving phase (weight adjustment volume per sample) respectively, and use gradient elution to carry out HPLC and analyze.

F) be used for deriving of lipid phase that GC/MS analyzes

The mixture that adds 140 μ l chloroforms, 38 μ l hydrochloric acid (37% the HCl aqueous solution), 320 μ l methyl alcohol and 20 μ l toluene in residue is to decompose methyl alcohol.Carefully shut sampling receptacle, 100 ℃ of reactions 2 hours.Follow evaporating solns and finish-drying throw out.

By in sealed tube, making the methoxy amination of carbonyl group in 1.5 hours 60 ℃ of reactions with 100 μ l methoxamine hydrochlorides (5mg/mL is in pyrimidine).Add the linear odd-numbered fatty acid mixture of 20 μ l so that time standard to be provided.At last, with 100 μ l N-methyl-N-(TMS)-2,2,2-trifluoroacetamide (MSTFA) was derived in 60 ℃ of reactions in 30 minutes in sealed tube.The final volume that is used for the GC injection is 220 μ l.

G) be used for deriving of polar phase that GC/MS analyzes

By in sealed tube, making the methoxy amination of carbonyl group in 1.5 hours 60 ℃ of reactions with 50 μ l methoxamine hydrochlorides (5mg/mL is in pyrimidine).Add the linear odd-numbered fatty acid mixture of 10 μ l so that time standard to be provided.At last, with 50 μ l N-methyl-N-(TMS)-2,2,2-trifluoroacetamide (MSTFA) was derived in 60 ℃ of reactions in 30 minutes in sealed tube.The final volume that is used for the GC injection is 110 μ l.

H) analysis of different plant samples.

Sample is measured with 20 sequences.Each sequence comprises 5 strain wild-types and 5 strain transgenic plant and 4 days or 8 days arid 5 strain wild-types and the 5 strain transgenic plant of handling under the collating condition.

With the peak height of every kind of analyte (metabolite) or peak area divided by the peak area of internal standard substance separately.With the fresh weight of sample separately with data normalization.With the mean value of the value that obtains divided by the wild-type plant of under collating condition, cultivating and in same sequence, analyzing, obtain so-called X multiple or ratio (seeing Table 7-14), its representative does not rely on the value of analytical sequence.These compare the state of metabolite concentration in the target plant than value representation and the concentration in the wild-type control plant.

Shown the plant metabolites results of screening that has transformed gene F19K19.13 in the table 5.

Table 2-5:

Table 2: to 3 age in week plant apply survival time of the Arabidopis thaliana that transforms behind the drought stress.Every other day visible is weighed arid patience.Survival time is the mean value that contrasts survival whole plants more of a specified duration than wild-type.Maximum duration is the single maximum duration that transforms plant more of a specified duration than wild-type contrast survival.

SEQ ID No.	Gene	Test plants	The average survival fate of After WT	Fate alive lives forever most
						Contrast	-	0	0
					1	At1g61800	24	0.83	3
3	At1g61810	24	0.83	3
					5	At5g65610	24	0.46	2
7	At5g65600	24	0.46	2
					9	At2g22530	24	2.08	6
11	At2g22540	24	2.08	6
					13	At3g57590	24	1.04	5
15	At3g62170	24	1.88	7

17	At3g62180	24	1.88	7
					19	At1g10120	24	1.21	4
21	At1g10130	24	1.21	4
					23	At1g07710	11	2.72	6
25	At1g07420	11	2.72	6
					27	At2g26890	45	2.33	6
29	At2g35050	45	2.33	6
					31	At5g44860	45	2.33	6
33	At5g44870	45	2.33	6
					35	At1g73490	24	0.88	5
37	At1g73480	24	0.88	5
					39	At5g22400	100	1.6	4
41	At5g22430	100	1.6	4
					43	At5g67210	100	1.6	4
45	At5g67220	100	1.6	4
					47	At1g15820	11	1.82	4
49	At1g15825	11	1.82	4
					51	At5g02470	19	0.95	3
53	At5g02480	19	0.95	3
					55	At3g49120	25	0.6	3
57	T2J13_50	25	0.6	3
					59	At2g25970	25	0.32	1
61	dI4495c	25	0.96	2
					63	dI4500c	25	0.96	2
65	At3g11170	25	0.76	2
					67	AT1G77310	25	0.76	2
69	AT1G77320	25	0.76	2
					71	At2g20210	25	1.08	2
73	At2g20200	25	1.08	2
					75	AT5g47370	25	1.08	2
77	At4g33200	25	0.88	3
					79	At5g45340	25	0.88	3
81	AT5G45810	25	0.72	2
					83	AT5G45820	25	0.72	2

85	At2g02370	24	2.46	7
					87	AT5G39460	24	1.38	5
89	MUL8.15	24	1.38	5
					91	F19K19.13 (At1g16540)	24	1.8	4
93	MLP3.2 (At3g07575)	49	4.1	6
					95	F13K23.5 (At1g12800)	18	1.7	4
97	MYJ24.7 (At5g23080)	24	1.0	4
					99	MBB18.23 (At5g38680)	24	1.1	3
101	At2g28470	15	1.2	4
					103	F9F8.1 (At3g11210)	27	1.0	3
105	F17C15_150 (At5g03730)	24	1.3	5
					107	At2g42690	24	0.9	4
109	At4g31810	48	8.3	12
					111	At4g31820	48	8.3	12

Table 3: to 3 age in week plant apply in the Arabidopis thaliana that transforms behind the drought stress photosynthesis output of measuring by chlorophyll fluorescence.Photosynthesis output (Y) is measured and be reported as to laggard in the ranks having a rest stopping to water.Value is the mean value of the plant selected at random of 5 strains.These values and MC24 strain system compare as a reference.

SEQ ID No.	Gene	The photosynthesis output of watering back 6 days at last	MC2 4 references	The photosynthesis output of watering back 10 days at last	MC2 4 references	The photosynthesis output of watering back 14 days at last	MC2 4 references
1	At1g61800	751	623	401	100
								3	At1g61810	751	623	401	100

5	At5g65610	770	757	552	610
								7	At5g65600	770	757	552	610
9	At2g22530	762	757	775	610	134	16
								11	At2g22540	762	757	775	610	134	16
13	At3g57590	770	757	757	610
								15	At3g62170	766	757	734	610	152	16
17	At3g62180	766	757	734	610	152	16
								23	At1g07710	779	757	752	610	305	16
25	At1g07420	779	757	752	610	305	16
								27	At2g26890	779	765	730	413	292	54
29	At2g35050	764	765	730	413	292	54
								31	At5g44860	764	765	730	413	292	54
33	At5g44870	764	765	730	413	292	54
								35	At1g73490	764	314
37	At1g73480	757	314
								47	At1g15820	757	757	536	610	148	16
49	At1g15825	745	757	536	610	148	16
								51	At5g02470	745	783	290	210
53	At5g02480	775	783	290	210
								55	At3g49120	775	783	265	210
57	T2J13_50	787	783	265	210
								59	At2g25970	787	783	723	210
61	dI4495c	784	783	597	210
								63	dI4500c	784	783	597	210
65	At3g11170	784	783	666	210
								67	AT1G77310	763	783	666	210
69	AT1G77320	763	783	666	210
								71	At2g20210	763	783	731	210
73	At2g20200	762	783	731	210
								75	AT5g47370	762	783	731	210
77	At4g33200	762	783	409	210
								79	At5g45340	792	783	409	210
81	AT5G45810	792	783	585	210
								83	AT5G45820	766	783	585	210
85	At2g02370	766	750	734	576	186	31
								87	AT5G39460	723	750	735	576	143	31
89	MUL8.15	740	750	735	576	143	31
								91	F19K19.13	772	757	792	610

93	MLP3.2	786	794	774	413	696	54
								95	F13K23.5	734	623	574	100
97	MYJ24.7	775	757	699	610
								99	MBB18.23	767	757	748	610
101	At2g28470	747	623	305	100
								103	F9F8.1	731	765	798	794	676	413
105	F17C15_150	759	757	639	610	148	107
								107	At2g42690	736	623	206	100
109	At4g31810	779	794	776	413	740	54
								111	At4g31820	779	794	776	413	740	54

Table 4: the PCR product full edition that is used to identify the genomic locus of downward modulation

	Gene	PCR product length	Sequence length	Border	Method	Restriction Enzyme or deg. primer
1	At1g61800	700bp	584bp	LB	Joint	MunI
							1	At1g61800	700bp	588bp	LB	Joint	MunI
3	At1g61810	700bp	588bp	LB	Joint	MunI
							5	At5g65610	1500bp	603bp	LB	Joint	Psp1406I/Bsp119I
5	At5g65610	1000bp	697bp	RB	Joint	BglII
							5	At5g65610	1000bp	175bp	RB	Joint	BglII
7	At5g65600	1000bp	175bp	RB	Joint	BglII
							9	At2g22530	550bp	465bp	LB	Joint	Psp1406I/Bsp119I
9	At2g22530	550bp	444bp	LB	Joint	Psp1406I/Bsp119I
							11	At2g22540	550bp	444bp	LB	Joint	Psp1406I/Bsp119I
13	At3g57590	950bp	842bp	LB	Joint	SpeI
							13	At3g57590	900bp	863bp	LB	Joint	SpeI
15	At3g62170	700bp	450bp	LB	Joint	Psp1406I/Bsp119I
							15	At3g62170	700bp	419bp	LB	Joint	Psp1406I/Bsp119I
18	At3g62180	700bp	450bp	LB	Joint	Psp1406I/Bsp119I
							19	At1g10120	700bp	161bp	LB	Joint	Psp1406I/Bsp119I
19	At1g10120	700bp	161bp	LB	Joint	Psp1406I/Bsp119I
							19	At1g10120	1400bp	718bp	LB	Joint	SpeI
21	At1g10130	1400bp	718bp	LB	Joint	SpeI
							23	At1g07710	1300bp	628bp	LB	Joint	MunI
23	At1g07710	1300bp	678bp	LB	Joint	MunI
							25	At1g07420	350bp	251bp	RB	Joint	SpeI

25	At1g07420	350bp	252bp	RB	Joint	SpeI
							27	At2g26890	700bp	615bp	LB	Joint	Psp1406I/Bsp119I
27	At2g26890	1200bp	637bp	LB	Joint	MunI
							29	At2g35050	500bp	445bp	RB	Joint	Psp1406I/Bsp119I
31	At5g44860	400bp	299bp	RB	Joint	BglII
							31	At5g44860	400bp	297bp	RB	Joint	BglII
33	At5g44870	400bp	297bp	RB	Joint	BglII
							35	At1g73490	330bp	244bp	LB	Joint	SpeI
35	At1g73490	650bp	494bp	RB	Joint	MunI
							37	At1g73480	650bp	494bp	RB	Joint	MunI
39	At5g22400	850bp	378bp	LB	Joint	BglII
							41	At5g22430	850bp	378bp	LB	Joint	BglII
43	At5g67210	900bp	471bp	RB	TAIL	ADP3
							43	At5g67210	850bp	471bp	RB	TAIL	ADP6
45	At5g67220	850bp	471bp	RB	TAIL	ADP6
							47	At1g15820	600bp	456bp	LB	Joint	BglII
47	At1g15820	600bp	284bp	LB	Joint	BglII
							49	At1g15825	600bp	456bp	LB	Joint	BglII
51	At5g02470	2000bp	170bp	RB	Joint	BglII
							53	At5g02480	2000bp	170bp	RB	Joint	BglII
55	At3g49120	950bp	720bp	LB	Joint	MunI
							57	T2J13_50	950bp	720bp	LB	Joint	MunI
59	At2g25970	1100bp	684bp	LB	Joint	SpeI
							61	d14495c	580bp	455bp	LB	Joint	Psp1406I/Bsp 119I
63	d14500c	580bp	455bp	LB	Joint	Psp1406I/Bsp 119I
							65	At3g11170	300bp	207bp	LB	Joint	BglII
67	AT1G7731 0	900bp	401bp	LB	Tail	ADP8
							69	AT1G7732 0	900bp	401bp	LB	Tail	ADP8
71	At2g20210	460bp	358bp	RB	Joint	MunI
							73	At2g20200	460bp	358bp	RB	Joint	MunI
75	AT5g47370	800bp	607bp	LB	Joint	MunI
							77	At4g33200	800bp	566bp	LB	Joint	BglII
79	At5g45340	1200bp	558bp	RB	Joint	BglII
							81	AT5G4581 0	800bp	647bp	LB	Joint	BglII
83	AT5G4582	800bp	647bp	LB	Joint	BglII

	0
							85	At2g02370	1300bp	548bp	LB	Joint	Psp 1406I/Bsp 119I
87	AT5G3946 0	700bp	548bp	LB	Joint	BglII
							89	MUL8.15	700bp	548bp	LB	Joint	BglII
91	F19K19.13	400bp	254bp	LB	Joint	BglII
							91	F19K19.13	400bp	256bp	LB	Joint	BglII
91	F19K19.13	700bp	625bp	RB	Joint	SpeI
							91	F19K19.13	700bp	479bp	RB	Joint	SpeI
93	MLP3.2	300bp	212bp	LB	TAIL	ADP3
							93	MLP3.2	550bp	419bp	RB	Joint	BglII
93	MLP3.2	500bp	362bp	RB	Joint	MunI
							93	MLP3.2	450bp	361bp	RB	Joint	MunI
95	F13K23.5	1600bp	585bp	LB	Joint	MunI
							97	MYJ24.7	550bp	432bp	LB	Joint	BglII
99	MBB18.23	1000bp	458bp	LB	Joint	MunI
							99	MBB18.23	1000bp	723bp	LB	Joint	MunI
99	MBB18.23	550bp	442bp	LB	Joint	Psp1406I/Bsp119I
							99	MBB18.23	600bp	450bp	LB	Joint	Psp1406I/Bsp119I
101	At2g28470	1100	652	LB	Joint	Psp1406/Bsp119
							103	F9F8.1	1000bp	518bp	LB	Joint	SpeI
103	F9F8.1	1000bp	550bp	LB	Joint	SpeI
							105	F17C15_15 0	850bp	607bp	LB	Joint	BglII
105	F17C15_15 0	1100bp	402bp	LB	Joint	MunI
							107	At2g42690	500	444	LB	TAIL	ADP8
109	At4g31810	1100bp	590bp	LB	TAIL	ADP8
							109	At4g31810	650bp	579bp	RB	TAIL	ADP8
111	At4g31820	650bp	579bp	RB	TAIL	ADP8

Table 5: metabolic activity screening full edition

Wild-type F19K19.13

Metabolite	Contrast	4 days	8 days	Contrast	4 days	8 days
							2,3-dimethyl-5-leaf chloro chinol	1,00	0.50	0.54			1.92
2-hydroxyl Palmiticacid	1.00	1.11	1.25	1.33	1.59	1.93
							3,4-dihydroxyphenylalanine (=DOPA)	1.00	1.83	3.37

3-hydroxyl Palmiticacid	1.00
							The 5-oxyproline	1.00	1.56	1.81	2.42	2.89
L-Ala	1.00	0.64	0.64	1.45	1.25
							Alpha-linolenic acid (c18:3 (c9, c12, c15))	1.00	1.24	1.40	1.27	1.65	1.85
Alpha-tocopherol	1.00	1.05	1.14
							Aminoadipic acid	1.00	1.73	1.65
Anhydroglucose	1.00	1.02	1.16	1.42
							Arginine	1.00	0.39	0.33		0.85
Aspartic acid	1.00	2.72	2.96	2.94	4.27	10.08
							β-A Piaohuluobusuquan	1.00	1.21	1.22
β-Hu Luobusu	1.00	1.25	1.26			1.91
							β-sitosterol	1.00	1.39	1.51			1.82
5,8-dimethyl tocol	1.00	0.54	0.60			3.13
							Palmiticacid	1.00	1.07	1.25
(cis of Δ-7-), 10-cis) hexadecadienoic acid (c16:2me)	1.00	1.01	1.04
							Hiragonic acid (c16:3)	1.00	1.19	1.29		1.46	1.57
Margaric acid (c17:0)	1.00	1.19	1.28	1.24		1.79
							Δ-15-cis nervonic acid	1.00	1.16	1.34	1.13	1.71	1.81
Brassicasterin	1.00	1.32	1.65	1.75	2.46	3.21
							Cerinic acid (c26:0)	1.00	0.74	1.00	0.38
Citrulline	1.00	0.51	0.33	1.88	1.70
							Cryptoxanthin	1.00	1.00	0.90
Eicosenoic acid (20:1)	1.00	0.81	0.81
							Forulic acid	1.00	1.37	1.47	1.66	2.62
Fructose	1.00	14.10	19.78
							Fumaric acid	1.00	5.19	9.33
Semi-lactosi	1.00	1.29	1.42
							γ-An Jidingsuan	1.00	1.16	1.32	1.62	2.21	5.00
Gamma-Tocopherol	1.00	0.54	0.60			1.69
							Gluconic acid	1.00	2.24	3.08
Glucose	1.00	14.97	20.73
							Glutamine	1.00	0.98	1.17	2.88	4.90
L-glutamic acid	1.00	2.01	2.69	2.49	6.81

R-Glyceric acid	1.00	1.87	2.04	0.85	1.21
							Glycerose	1.00	1.11	1.12	1.56	2.59	4.80
Glycerine	1.00	1.22	1.29
							Glycerol-3-phosphate	1.00	1.86	2.41	1.90
Glycine	1.00	0.25	0.26
							Homoserine	1.00	0.61	0.69
Inositol	1.00	4.19	6.32	2.02	5.03	21.68
							Isoleucine	1.00	1.28	1.66	1.44	1.96	8.02
Isomaltose	1.00	2.63	3.02
							Isopentenylpyrophosphate	1.00	1.80	2.62	1.79	3.48
Leucine	1.00	1.34	1.74	1.59	2.33	6.58
							Lignoceric acid (c24:0)	1.00	0.91	1.02
Linolic acid (c18:2 (c9, c12))	1.00	1.19	1.38		1.41	1.66
							Luteine	1.00	1.26	1.33
Oxysuccinic acid					1.68	1.84
							Seminose	1.00	2.91	3.46	1.72
Triacontanoic acid	1.00	16.40	17.80	2.56		79.91
							Methionine(Met)	1.00	1.26	1.23
The methyl furan galactoside	1.00	1.13	1.12	1.64
							The methyl galactopyranoside	1.00	1.24	1.49		1.68	1.84
Ornithine	1.00	1.25	1.36		1.37	1.70
							Palmiticacid (c16:0)	1.00	1.16	1.38	1.23	1.61	1.98
Phenylalanine	1.00	0.92	1.10
							Phosphoric acid	1.00	2.15	2.73	2.37	7.31	11.38
Proline(Pro)	1.00	1.23	4.77	2.23	4.26
							Putrescine	1.00		0.39
Pyruvic acid	1.00	1.30	1.21
							Raffinose	1.00	17.04	74.78		7.06
Ribonic acid	1.00	2.32	3.43
							Serine	1.00	0.98	1.13	1.47	1.68
Shikimin	1.00	1.11	1.07	0.85	0.79	0.71
							Sinapinic acid	1.00	2.74	3.44	1.30	1.87
Stearic acid (c18:0)	1.00	1.18	1.35	1.16	1.36	1.79
							Succsinic acid	1.00	2.98	3.49	2.01

Sucrose	1.00	1.42	1.70	1.46	2.60	3.87
							Threonine	1.00	1.26	1.68	3.28	4.06
Tryptophane	1.00	1.13	1.89		1.86
							Tyrosine	1.00	1.56	2.01	1.46
Ubiquinone	1.00	1.02	1.20			3.13
							Udp-glucose	1.00	1.53	1.94	2.02	4.85
Xie Ansuan	1.00	0.98	1.18	1.51	1.75	3.18
							Zeaxanthin	1.00	1.27	1.34
2,3-dimethyl-5-leaf chloro chinol	1.00	0.50	0.54			1.92

Sequence table

<210>1

<211>1167

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(1167)

<400>1

atg ctt tct tca atc aaa cca tct tcg tct tcc ttc tcc acc gct atc 48

Met Leu Ser Ser Ile Lys Pro Ser Ser Ser Ser Phe Ser Thr Ala Ile

1 5 10 15

tcc ggc agc gta agg cgg tca att cct aca gag ctc aag ttt tct cct 96

Ser Gly Ser Val Arg Arg Ser Ile Pro Thr Lys Leu Lys Phe Ser Pro

20 25 30

tta ctc atc atc aaa aac tgc cat aac caa agc ttt aac gct aat gtt 144

Leu Leu Ile Ile Lys Asn Cys His Asn Gln Ser Phe Asn Ala Asn Val

35 40 45

gtc tcc cat caa aag cct cta cac att tca tct gct tcg aat ttc aag 192

Val Ser His Gln Lys Pro Leu His Ile Ser Ser Ala Ser Asn Phe Lys

50 55 60

cgt gag gtc aaa gtc gaa gct tac gag gcc gat cgt tcc cgt cca ctg 240

Arg Glu Val Lys Val Glu Ala Tyr Glu Ala Asp Arg Ser Arg Pro Leu

65 70 75 80

gac atc aac atc gag ctt ccc gat gaa caa tcc gcg cag aaa ctg aaa 288

Asp Ile Asn Ile Glu Leu Pro Asp Glu Gln Ser Ala Gln Lys Leu Lys

85 90 95

atc gga atc tac ttc gca act tgg tgg gca ctt aac gtt gtc ttc aac 336

Ile Gly Ile Tyr Phe Ala Thr Trp Trp Ala Leu Asn Val Val Phe Asn

100 105 110

atc tac aac aag aaa gtc ctc aat gct ttt cct tac ccg tgg ctt act 384

Ile Tyr Asn Lys Lys Val Leu Asn Ala Phe Pro Tyr Pro Trp Leu Thr

115 120 125

tcg acg ttg tct ctc gct tgt ggt tct ttg atg atg ctt gtc tct tgg 432

Ser Thr Leu Ser Leu Ala Cys Gly Ser Leu Met Met Leu Val Ser Trp

130 135 140

gct act aga atc gca gat gct cct aaa act gat ctc gag ttc tgg aaa 480

Ala Thr Arg Ile Ala Asp Ala Pro Lys Thr Asp Leu Glu Phe Trp Lys

145 150 155 160

act ctg ttc ccg gtc gct gta gca cac acg ata gga cac gtt gca gca 528

Thr Leu Phe Pro Val Ala Val Ala His Thr Ile Gly His Val Ala Ala

165 170 175

aca gtg agt atg tca aaa gta gca gtt tcc ttc aca cac atc att aaa 576

Thr Val Ser Met Ser Lys Val Ala Val Ser Phe Thr His Ile Ile Lys

180 185 190

agt ggt gaa cca gct ttc agt gtc tta gtc tca aga ttc ttc atg gga 624

Ser Gly Glu Pro Ala Phe Ser Val Leu Val Ser Arg Phe Phe Met Gly

195 200 205

gag act ttc cct ctt cct gtc tat ctc tct ctc tta cca atc atc gga 672

Glu Thr Phe Pro Leu Pro Val Tyr Leu Ser Leu Leu Pro Ile Ile Gly

210 215 220

ggc tgc gct ctc gcg gcc atc acc gag ctt aac ttc aac atc act ggg 720

Gly Cys Ala Leu Ala Ala Ile Thr Glu Leu Asn Phe Asn Ile Thr Gly

225 230 235 240

ttt atg ggg gca atg ata tcg aat ttg gca ttt gtg ttc cgg aat atc 768

Phe Met Gly Ala Met Ile Ser Asn Leu Ala Phe Val Phe Arg Asn Ile

245 250 255

ttt tcg aag aaa ggg atg aaa ggg aag tca gtg agc gga atg aac tac 816

Phe Ser Lys Lys Gly Met Lys Gly Lys Ser Val Ser Gly Met Asn Tyr

260 265 270

tac gct tgc tta tcg atg atg tct ctt gtg atc ctc act cca ttt tct 864

Tyr Ala Cys Leu Ser Met Met Ser Leu Val Ile Leu Thr Pro Phe Ser

275 280 285

att gcc gtg gaa ggt cct caa atg tgg gct gct ggt tgg caa aat gcg 912

Ile Ala Val Glu Gly Pro Gln Met Trp Ala Ala Gly Trp Gln Asn Ala

290 295 300

gtt tct caa gtc gga cca aac ttt gtc tgg tgg gta gtg gca caa agt 960

Val Ser Gln Val Gly Pro Asn Phe Val Trp Trp Val Val Ala Gln Ser

305 310 315 320

gtg ttt tac cat ttg tac aat cag gtc tca tac atg tca tta gac cag 1008

Val Phe Tyr His Leu Tyr Asn Gln Val Ser Tyr Met Ser Leu Asp Gln

325 330 335

att tcg ccg tta act ttc agt atc ggt aat acg atg aag cgg att tcc 1056

Ile Ser Pro Leu Thr Phe Ser Ile Gly Asn Thr Met Lys Arg Ile Ser

340 345 350

gtt att gtt gca tcg atc atc att ttc cat acc ccg att caa ccg gtt 1104

Val Ile Val Ala Ser Ile Ile Ile Phe His Thr Pro Ile Gln Pro Val

355 360 365

aat gcc ctc ggt gct gcc att gcg att ttt gga act ttc ctc tac tca 1152

Asn Ala Leu Gly Ala Ala Ile Ala Ile Phe Gly Thr Phe Leu Tyr Ser

370 375 380

cag gcg aag cag tga 1167

Gln Ala Lys Gln

385

<210>2

<211>388

<212>PRT

＜213〉Arabidopis thaliana

<400>2

Met Leu Ser Ser Ile Lys Pro Ser Ser Ser Ser Phe Ser Thr Ala Ile

1 5 10 15

Ser Gly Ser Val Arg Arg Ser Ile Pro Thr Lys Leu Lys Phe Ser Pro

20 25 30

Leu Leu Ile Ile Lys Asn Cys His Asn Gln Ser Phe Asn Ala Asn Val

35 40 45

Val Ser His Gln Lys Pro Leu His Ile Ser Ser Ala Ser Asn Phe Lys

50 55 60

Arg Glu Val Lys Val Glu Ala Tyr Glu Ala Asp Arg Ser Arg Pro Leu

65 70 75 80

Asp Ile Asn Ile Glu Leu Pro Asp Glu Gln Ser Ala Gln Lys Leu Lys

85 90 95

Ile Gly Ile Tyr Phe Ala Thr Trp Trp Ala Leu Asn Val Val Phe Asn

100 105 110

Ile Tyr Asn Lys Lys Val Leu Asn Ala Phe Pro Tyr Pro Trp Leu Thr

115 120 125

Ser Thr Leu Ser Leu Ala Cys Gly Ser Leu Met Met Leu Val Ser Trp

130 135 140

Ala Thr Arg Ile Ala Asp Ala Pro Lys Thr Asp Leu Glu Phe Trp Lys

145 150 155 160

Thr Leu Phe Pro Val Ala Val Ala His Thr Ile Gly His Val Ala Ala

165 170 175

Thr Val Ser Met Ser Lys Val Ala Val Ser Phe Thr His Ile Ile Lys

180 185 190

Ser Gly Glu Pro Ala Phe Ser Val Leu Val Ser Arg Phe Phe Met Gly

195 200 205

Glu Thr Phe Pro Leu Pro Val Tyr Leu Ser Leu Leu Pro Ile Ile Gly

210 215 220

Gly Cys Ala Leu Ala Ala Ile Thr Glu Leu Asn Phe Asn Ile Thr Gly

225 230 235 240

Phe Met Gly Ala Met Ile Ser Asn Leu Ala Phe Val Phe Arg Asn Ile

245 250 255

Phe Ser Lys Lys Gly Met Lys Gly Lys Ser Val Ser Gly Met Asn Tyr

260 265 270

Tyr Ala Cys Leu Ser Met Met Ser Leu Val Ile Leu Thr Pro Phe Ser

275 280 285

Ile Ala Val Glu Gly Pro Gln Met Trp Ala Ala Gly Trp Gln Asn Ala

290 295 300

Val Ser Gln Val Gly Pro Asn Phe Val Trp Trp Val Val Ala Gln Ser

305 310 315 320

Val Phe Tyr His Leu Tyr Asn Gln Val Ser Tyr Met Ser Leu Asp Gln

325 330 335

Ile Ser Pro Leu Thr Phe Ser Ile Gly Asn Thr Met Lys Arg Ile Ser

340 345 350

Val Ile Val Ala Ser Ile Ile Ile Phe His Thr Pro Ile Gln Pro Val

355 360 365

Asn Ala Leu Gly Ala Ala Ile Ala Ile Phe Gly Thr Phe Leu Tyr Ser

370 375 380

Gln Ala Lys Gln

385

<210>3

<211>1563

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(1563)

<400>3

atg aaa aat tta acc agt ttc gta att gtt atc tta cta caa agc tta 48

Met Lys Asn Leu Thr Ser Phe Val Ile Val Ile Leu Leu Gln Ser Leu

1 5 10 15

ttg ttt cat gta tat ggt cgt cat caa agt tct tcg aag aac att tta 96

Leu Phe His Val Tyr Gly Arg His Gln Ser Ser Ser Lys Asn Ile Leu

20 25 30

gta gat tca tct cca ttt cct tct gat ttc ctc ttt ggt aca gct tct 144

Val Asp Ser Ser Pro Phe Pro Ser Asp Phe Leu Phe Gly Thr Ala Ser

35 40 45

tcc gct tac cag tat gaa ggt gcg ttc ttg act gat gga aaa agt ttg 192

Ser Ala Tyr Gln Tyr Glu Gly Ala Phe Leu Thr Asp Gly Lys Ser Leu

50 55 60

aac aat tgg gat gtc ttt acg cat aaa aat cct ggg aaa ata ctt gac 240

Asn Asn Trp Asp Val Phe Thr His Lys Asn Pro Gly Lys Ile Leu Asp

65 70 75 80

aag aac aat gca gat aga gct gtg gac caa tat aat cga ttt ttg gag 288

Lys Asn Asn Ala Asp Arg Ala Val Asp Gln Tyr Asn Arg Phe Leu Glu

85 90 95

gac atc caa tta atg tct ttt ctt gga gtg aac agt tat agg ttt tca 336

Asp Ile Gln Leu Met Ser Phe Leu Gly Val Asn Ser Tyr Arg Phe Ser

100 105 110

att tct tgg tgt aga atc tta cct aga gga aga ttt gga gag att aat 384

Ile Ser Trp Cys Arg Ile Leu Pro Arg Gly Arg Phe Gly Glu Ile Asn

115 120 125

tat ttg ggg ata aag tat tac aac ata ttt atc gat gct ctc att agt 432

Tyr Leu Gly Ile Lys Tyr Tyr Asn Ile Phe Ile Asp Ala Leu Ile Ser

130 135 140

aga ggg att aaa cca ttt gtg acg ttg aac cat gtc gac tat cct caa 480

Arg Gly Ile Lys Pro Phe Val Thr Leu Asn His Val Asp Tyr Pro Gln

145 150 155 160

gaa ctc gag gac cgg ttt caa agt tgg tta aac ccc gag atg cag aag 528

Glu Leu Glu Asp Arg Phe Gln Ser Trp Leu Asn Pro Glu Met Gln Lys

165 170 175

gaa ttt ggt tat tta gcc gat ata tgt ttc aag cat ttt gga aac cga 576

Glu Phe Gly Tyr Leu Ala Asp Ile Cys Phe Lys His Phe Gly Asn Arg

180 185 190

gtt aaa tac tgg acc acg ctt aac gaa cca aat caa caa tta atc tta 624

Val Lys Tyr Trp Thr Thr Leu Asn Glu Pro Asn Gln Gln Leu Ile Leu

195 200 205

ggc tat cta aca ggt aaa ttt cca ccc tct cgt tgc tcc tcg cca tac 672

Gly Tyr Leu Thr Gly Lys Phe Pro Pro Ser Arg Cys Ser Ser Pro Tyr

210 215 220

ggg aac tgt agc cag ggg aat tca gaa acc gaa cct ttc ata gcc gca 720

Gly Asn Cys Ser Gln Gly Asn Ser Glu Thr Glu Pro Phe Ile Ala Ala

225 230 235 240

cat aac atg atc ctt gca cac gca aaa gcg gtt aac ata tac aag acc 768

His Asn Met Ile Leu Ala His Ala Lys Ala Val Asn Ile Tyr Lys Thr

245 250 255

aaa tat cag aaa gaa caa aag gga agc att ggc att gtg gta caa aca 816

Lys Tyr Gln Lys Glu Gln Lys Gly Ser Ile Gly Ile Val Val Gln Thr

260 265 270

tca tgg ttt gaa ccc att agt gat tcc aat gcg gat aaa gaa gct gct 864

Ser Trp Phe Glu Pro Ile Ser Asp Ser Asn Ala Asp Lys Glu Ala Ala

275 280 285

gag aga gct caa tca ttt tac tct aat tgg att cta gat ccc gtt ata 912

Glu Arg Ala Gln Ser Phe Tyr Ser Asn Trp Ile Leu Asp Pro Val Ile

290 295 300

tat ggg aaa tat cca aaa gaa atg gta gat att ctt gga cca gcc ttg 960

Tyr Gly Lys Tyr Pro Lys Glu Met Val Asp Ile Leu Gly Pro Ala Leu

305 310 315 320

cca caa ttt tcg agc aat gaa gtg aag aac tta gag aag tcg agg gca 1008

Pro Gln Phe Ser Ser Asn Glu Val Lys Asn Leu Glu Lys Ser Arg Ala

325 330 335

gat ttc gtt ggt att aat cac tat aca agt tac ttc att caa gat tgt 1056

Asp Phe Val Gly Ile Asn His Tyr Thr Ser Tyr Phe Ile Gln Asp Cys

340 345 350

ttg acc tct gct tgt aat act gga cat gga gct ttt aag gcc gaa gga 1104

Leu Thr Ser Ala Cys Asn Thr Gly His Gly Ala Phe Lys Ala Glu Gly

355 360 365

tat gct ctc aag tta gac cgg aaa ggc aat gtt act ata gga gaa ctt 1152

Tyr Ala Leu Lys Leu Asp Arg Lys Gly Asn Val Thr Ile Gly Glu Leu

370 375 380

acc gat gta aat tgg cag cat att gat ccc aca gga ttc cac aag atg 1200

Thr Asp Val Asn Trp Gln His Ile Asp Pro Thr Gly Phe His Lys Met

385 390 395 400

ctg aat tat tta aaa gat agg tac cca aac atg cca atg ttc ata act 1248

Leu Asn Tyr Leu Lys Asp Arg Tyr Pro Asn Met Pro Met Phe Ile Thr

405 410 415

gaa aac ggt ttt gga gac ttg caa aaa cct gaa aca acg gat aaa gaa 1296

Glu Asn Gly Phe Gly Asp Leu Gln Lys Pro Glu Thr Thr Asp Lys Glu

420 425 430

ctt cta aat gat aca aaa agg atc caa tac atg agt gga tac tta gaa 1344

Leu Leu Asn Asp Thr Lys Arg Ile Gln Tyr Met Ser Gly Tyr Leu Glu

435 440 445

gct tta caa gca gca atg agg gat gga gca aat gtg aag ggt tat ttt 1392

Ala Leu Gln Ala Ala Met Arg Asp Gly Ala Asn Val Lys Gly Tyr Phe

450 455 460

gtg tgg tca cta tta gac aat ttt gag tgg ttg ttc gga tac aag gtt 1440

Val Trp Ser Leu Leu Asp Asn Phe Glu Trp Leu Phe Gly Tyr Lys Val

465 470 475 480

cgg ttt ggt cta ttc cat gtg gat cta aca act ctt aaa agg tca ccg 1488

Arg Phe Gly Leu Phe His Val Asp Leu Thr Thr Leu Lys Arg Ser Pro

485 490 495

aaa caa tca gct tca tgg tac aaa aat tat atc gaa gaa cac gtg aat 1536

Lys Gln Ser Ala Ser Trp Tyr Lys Asn Tyr Ile Glu Glu His Val Asn

500 505 510

cga aga gat atc gta gat aat tat taa 1563

Arg Arg Asp Ile Val Asp Asn Tyr

515 520

<210>4

<211>520

<212>PRT

＜213〉Arabidopis thaliana

<400>4

Met Lys Asn Leu Thr Ser Phe Val Ile Val Ile Leu Leu Gln Ser Leu

1 5 10 15

Leu Phe His Val Tyr Gly Arg His Gln Ser Ser Ser Lys Asn Ile Leu

20 25 30

Val Asp Ser Ser Pro Phe Pro Ser Asp Phe Leu Phe Gly Thr Ala Ser

35 40 45

Ser Ala Tyr Gln Tyr Glu Gly Ala Phe Leu Thr Asp Gly Lys Ser Leu

50 55 60

Asn Asn Trp Asp Val Phe Thr His Lys Asn Pro Gly Lys Ile Leu Asp

65 70 75 80

Lys Asn Asn Ala Asp Arg Ala Val Asp Gln Tyr Asn Arg Phe Leu Glu

85 90 95

Asp Ile Gln Leu Met Ser Phe Leu Gly Val Asn Ser Tyr Arg Phe Ser

100 105 110

Ile Ser Trp Cys Arg Ile Leu Pro Arg Gly Arg Phe Gly Glu Ile Asn

115 120 125

Tyr Leu Gly Ile Lys Tyr Tyr Asn Ile Phe Ile Asp Ala Leu Ile Ser

130 135 140

Arg Gly Ile Lys Pro Phe Val Thr Leu Asn His Val Asp Tyr Pro Gln

145 150 155 160

Glu Leu Glu Asp Arg Phe Gln Ser Trp Leu Asn Pro Glu Met Gln Lys

165 170 175

Glu Phe Gly Tyr Leu Ala Asp Ile Cys Phe Lys His Phe Gly Asn Arg

180 185 190

Val Lys Tyr Trp Thr Thr Leu Asn Glu Pro Asn Gln Gln Leu Ile Leu

195 200 205

Gly Tyr Leu Thr Gly Lys Phe Pro Pro Ser Arg Cys Ser Ser Pro Tyr

210 215 220

Gly Asn Cys Ser Gln Gly Asn Ser Glu Thr Glu Pro Phe Ile Ala Ala

225 230 235 240

His Asn Met Ile Leu Ala His Ala Lys Ala Val Asn Ile Tyr Lys Thr

245 250 255

Lys Tyr Gln Lys Glu Gln Lys Gly Ser Ile Gly Ile Val Val Gln Thr

260 265 270

Ser Trp Phe Glu Pro Ile Ser Asp Ser Asn Ala Asp Lys Glu Ala Ala

275 280 285

Glu Arg Ala Gln Ser Phe Tyr Ser Asn Trp Ile Leu Asp Pro Val Ile

290 295 300

Tyr Gly Lys Tyr Pro Lys Glu Met Val Asp Ile Leu Gly Pro Ala Leu

305 310 315 320

Pro Gln Phe Ser Ser Asn Glu Val Lys Asn Leu Glu Lys Ser Arg Ala

325 330 335

Asp Phe Val Gly Ile Asn His Tyr Thr Ser Tyr Phe Ile Gln Asp Cys

340 345 350

Leu Thr Ser Ala Cys Asn Thr Gly His Gly Ala Phe Lys Ala Glu Gly

355 360 365

Tyr Ala Leu Lys Leu Asp Arg Lys Gly Asn Val Thr Ile Gly Glu Leu

370 375 380

Thr Asp Val Asn Trp Gln His Ile Asp Pro Thr Gly Phe His Lys Met

385 390 395 400

Leu Asn Tyr Leu Lys Asp Arg Tyr Pro Asn Met Pro Met Phe Ile Thr

405 410 415

Glu Asn Gly Phe Gly Asp Leu Gln Lys Pro Glu Thr Thr Asp Lys Glu

420 425 430

Leu Leu Asn Asp Thr Lys Arg Ile Gln Tyr Met Ser Gly Tyr Leu Glu

435 440 445

Ala Leu Gln Ala Ala Met Arg Asp Gly Ala Asn Val Lys Gly Tyr Phe

450 455 460

Val Trp Ser Leu Leu Asp Asn Phe Glu Trp Leu Phe Gly Tyr Lys Val

465 470 475 480

Arg Phe Gly Leu Phe His Val Asp Leu Thr Thr Leu Lys Arg Ser Pro

485 490 495

Lys Gln Ser Ala Ser Trp Tyr Lys Asn Tyr Ile Glu Glu His Val Asn

500 505 510

Arg Arg Asp Ile Val Asp Asn Tyr

515 520

<210>5

<211>753

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(753)

<400>5

atg gcg aca gag aga gag acg att gag agg aga gat caa agg att ctc 48

Met Ala Thr Glu Arg Glu Thr Ile Glu Arg Arg Asp Gln Arg Ile Leu

1 5 10 15

gtt tcc att gac gac gat ggt gga gga aga aag caa tca tca aag aca 96

Val Ser Ile Asp Asp Asp Gly Gly Gly Arg Lys Gln Ser Ser Lys Thr

20 25 30

aag aag ctt ctc aga tta cga atg aag aag acg gag aat ttc aga cga 144

Lys Lys Leu Leu Arg Leu Arg Met Lys Lys Thr Glu Asn Phe Arg Arg

35 40 45

cga cga cgc aag gtg ggc tca cgg tgg tgg gct ttt ttt tat ggg ccc 192

Arg Arg Arg Lys Val Gly Ser Arg Trp Trp Ala Phe Phe Tyr Gly Pro

50 55 60

ctt ctt tgc tac caa atc caa cca gaa aaa gtt tta tgc ttg aag ctc 240

Leu Leu Cys Tyr Gln Ile Gln Pro Glu Lys Val Leu Cys Leu Lys Leu

65 70 75 80

tcg tcg gtc gct aga gct gaa ttt gat ttg agg gag atg gaa aga gac 288

Ser Ser Val Ala Arg Ala Glu Phe Asp Leu Arg Glu Met Glu Arg Asp

85 90 95

aag gtt aat gat ttt gtg gtt aat ggc aag att aac aag tta att aag 336

Lys Val Asn Asp Phe Val Val Asn Gly Lys Ile Asn Lys Leu Ile Lys

100 105 110

tta tta tca aat aga gga tcc cat ata gta act tta cga gag cat aac 384

Leu Leu Ser Asn Arg Gly Ser His Ile Val Thr Leu Arg Glu His Asn

115 120 125

gta gac gaa cca aca cta acc aca aaa ctt agg ggt gca tac aaa aat 432

Val Asp Glu Pro Thr Leu Thr Thr Lys Leu Arg Gly Ala Tyr Lys Asn

130 135 140

aaa att gaa aaa aca gag cat aga tca gtg atc cgg act ctg ttt tgg 480

Lys Ile Glu Lys Thr Glu His Arg Ser Val Ile Arg Thr Leu Phe Trp

145 150 155 160

aag atc tct aaa aag ctt agg atc atc atc ggc atc gcg cag ata gaa 528

Lys Ile Ser Lys Lys Leu Arg Ile Ile Ile Gly Ile Ala Gln Ile Glu

165 170 175

acc atc atc gaa att ctg cga ata gct cac agc atc gta gtg aaa cgt 576

Thr Ile Ile Glu Ile Leu Arg Ile Ala His Ser Ile Val Val Lys Arg

180 185 190

ctt gtc ttg ttg ttg tgg tta ctg att cga gcg tac tct gtt tct tct 624

Leu Val Leu Leu Leu Trp Leu Leu Ile Arg Ala Tyr Ser Val Ser Ser

195 200 205

gta tgg atg aga caa gat aac ggc gaa ctc aag cat cga ccg gtg gag 672

Val Trp Met Arg Gln Asp Asn Gly Glu Leu Lys His Arg Pro Val Glu

210 215 220

tac gat tgt cta cgg ttg tcg gcg aga act cta tcc att tct tgc aaa 720

Tyr Asp Cys Leu Arg Leu Ser Ala Arg Thr Leu Ser Ile Ser Cys Lys

225 230 235 240

aat aaa aga gag att gtt tta ttg att gag tga 753

Asn Lys Arg Glu Ile Val Leu Leu Ile Glu

245 250

<210>6

<211>250

<212>PRT

＜213〉Arabidopis thaliana

<400>6

Met Ala Thr Glu Arg Glu Thr Ile Glu Arg Arg Asp Gln Arg Ile Leu

1 5 10 15

Val Ser Ile Asp Asp Asp Gly Gly Gly Arg Lys Gln Ser Ser Lys Thr

20 25 30

Lys Lys Leu Leu Arg Leu Arg Met Lys Lys Thr Glu Asn Phe Arg Arg

35 40 45

Arg Arg Arg Lys Val Gly Ser Arg Trp Trp Ala Phe Phe Tyr Gly Pro

50 55 60

Leu Leu Cys Tyr Gln Ile Gln Pro Glu Lys Val Leu Cys Leu Lys Leu

65 70 75 80

Ser Ser Val Ala Arg Ala Glu Phe Asp Leu Arg Glu Met Glu Arg Asp

85 90 95

Lys Val Asn Asp Phe Val Val Asn Gly Lys Ile Asn Lys Leu Ile Lys

100 105 110

Leu Leu Ser Asn Arg Gly Ser His Ile Val Thr Leu Arg Glu His Asn

115 120 125

Val Asp Glu Pro Thr Leu Thr Thr Lys Leu Arg Gly Ala Tyr Lys Asn

130 135 140

Lys Ile Glu Lys Thr Glu His Arg Ser Val Ile Arg Thr Leu Phe Trp

145 150 155 160

Lys Ile Ser Lys Lys Leu Arg Ile Ile Ile Gly Ile Ala Gln Ile Glu

165 170 175

Thr Ile Ile Glu Ile Leu Arg Ile Ala His Ser Ile Val Val Lys Arg

180 185 190

Leu Val Leu Leu Leu Trp Leu Leu Ile Arg Ala Tyr Ser Val Ser Ser

195 200 205

Val Trp Met Arg Gln Asp Asn Gly Glu Leu Lys His Arg Pro Val Glu

210 215 220

Tyr Asp Cys Leu Arg Leu Ser Ala Arg Thr Leu Ser Ile Ser Cys Lys

225 230 235 240

Asn Lys Arg Glu Ile Val Leu Leu Ile Glu

245 250

<210>7

<211>2028

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(2028)

<400>7

atg ctt tat ttc att ttc tgt caa aat ctt tct tct tcc tcc tct atg 48

Met Leu Tyr Phe Ile Phe Cys Gln Asn Leu Ser Ser Ser Ser Ser Met

1 5 10 15

tct aat tca atc ttg ttc ctt tcc ctt ttc ctc ttc ctc cct ttt gtt 96

Ser Asn Ser Ile Leu Phe Leu Ser Leu Phe Leu Phe Leu Pro Phe Val

20 25 30

gtt gat tca ctt tac ttc aac ttt aca agt ttt cga caa ggt gat ccc 144

Val Asp Ser Leu Tyr Phe Asn Phe Thr Ser Phe Arg Gln Gly Asp Pro

35 40 45

ggg gac ata ttt tac cac ggg gat gca act ccc gat gaa gat gga aca 192

Gly Asp Ile Phe Tyr His Gly Asp Ala Thr Pro Asp Glu Asp Gly Thr

50 55 60

gtg aac ttc aac aac gct gaa caa aca tct cag gtt ggt tgg att act 240

Val Asn Phe Asn Asn Ala Glu Gln Thr Ser Gln Val Gly Trp Ile Thr

65 70 75 80

tat tca aag aag gtg cct ata tgg agt cat aaa act ggt aag gct tca 288

Tyr Ser Lys Lys Val Pro Ile Trp Ser His Lys Thr Gly Lys Ala Ser

85 90 95

gat ttc agc acc agt ttc tcc ttc aaa atc gat gct cgt aat ctt tca 336

Asp Phe Ser Thr Ser Phe Ser Phe Lys Ile Asp Ala Arg Asn Leu Ser

100 105 110

gca gat ggt cat gga atc tgt ttc ttt ctt gct cct atg gga gct caa 384

Ala Asp Gly His Gly Ile Cys Phe Phe Leu Ala Pro Met Gly Ala Gln

115 120 125

cta cct gca tac tca gtt ggt ggt ttc ttg aat ttg ttc act cgg aag 432

Leu Pro Ala Tyr Ser Val Gly Gly Phe Leu Asn Leu Phe Thr Arg Lys

130 135 140

aat aat tac tca tct tcg ttt ccg cta gtt cat gtt gaa ttt gac acg 480

Asn Asn Tyr Ser Ser Ser Phe Pro Leu Val His Val Glu Phe Asp Thr

145 150 155 160

ttt aac aat cca gga tgg gat cct aat gat gtt gga tct cat gtt ggg 528

Phe Asn Asn Pro Gly Trp Asp Pro Asn Asp Val Gly Ser His Val Gly

165 170 175

atc aat aac aac tct ctt gtt tct tcc aac tac act tct tgg aat gca 576

Ile Asn Asn Asn Ser Leu Val Ser Ser Asn Tyr Thr Ser Trp Asn Ala

180 185 190

agc tct cac agc caa gat att tgt cat gca aag atc tct tat gat tcc 624

Ser Ser His Ser Gln Asp Ile Cys His Ala Lys Ile Ser Tyr Asp Ser

195 200 205

gtg acc aag aat tta agt gtg act tgg gct tat gag cta aca gca acc 672

Val Thr Lys Asn Leu Ser Val Thr Trp Ala Tyr Glu Leu Thr Ala Thr

210 215 220

tct gat cct aag gag agt tca agc ctt tct tac atc atc gac ctc gct 720

Ser Asp Pro Lys Glu Ser Ser Ser Leu Ser Tyr Ile Ile Asp Leu Ala

225 230 235 240

aag gtt ctg cca tca gat gtt atg ttc ggg ttt ata gct gcc gct ggg 768

Lys Val Leu Pro Ser Asp Val Met Phe Gly Phe Ile Ala Ala Ala Gly

245 250 255

aca aac aca gag gaa cat aga ctt tta tca tgg gag ctc agt tca agt 816

Thr Asn Thr Glu Glu His Arg Leu Leu Ser Trp Glu Leu Ser Ser Ser

260 265 270

ttg gac agc gac aaa gcc gat agc agg ata gga cta gta att ggc att 864

Leu Asp Ser Asp Lys Ala Asp Ser Arg Ile Gly Leu Val Ile Gly Ile

275 280 285

tcg gct tcc ggg ttt gtt ttt ctg acg ttt atg gtc atc aca acc gtg 912

Ser Ala Ser Gly Phe Val Phe Leu Thr Phe Met Val Ile Thr Thr Val

290 295 300

gtg gtt tgg tca cgg aag caa aga aag aag aaa gaa aga gat ata gaa 960

Val Val Trp Ser Arg Lys Gln Arg Lys Lys Lys Glu Arg Asp Ile Glu

305 310 315 320

aac atg ata tcg ata aac aaa gac ctt gaa agg gaa gca gga cca aga 1008

Asn Met Ile Ser Ile Asn Lys Asp Leu Glu Arg Glu Ala Gly Pro Arg

325 330 335

aag ttt tct tat aag gat ctt gtg tca gca acc aac aga ttc tca agc 1056

Lys Phe Ser Tyr Lys Asp Leu Val Ser Ala Thr Asn Arg Phe Ser Ser

340 345 350

cat aga aag cta ggt gaa gga gga ttt gga gca gtt tat gaa gga aac 1104

His Arg Lys Leu Gly Glu Gly Gly Phe Gly Ala Val Tyr Glu Gly Asn

355 360 365

ttg aaa gaa atc aat aca atg gtt gcg gtg aag aaa cta tcc ggt gat 1152

Leu Lys Glu Ile Asn Thr Met Val Ala Val Lys Lys Leu Ser Gly Asp

370 375 380

tca agg cag gga aag aac gag ttt tta aac gaa gtt aag atc atc agc 1200

Ser Arg Gln Gly Lys Asn Glu Phe Leu Asn Glu Val Lys Ile Ile Ser

385 390 395 400

aaa ctg aga cat cga aac ctc gtg cag ctc atc ggt tgg tgt aat gag 1248

Lys Leu Arg His Arg Asn Leu Val Gln Leu Ile Gly Trp Cys Asn Glu

405 410 415

aaa aat gag ttc ttg ctg ata tat gag tta gtt cca aat ggt agc ttg 1296

Lys Asn Glu Phe Leu Leu Ile Tyr Glu Leu Val Pro Asn Gly Ser Leu

420 425 430

aac tct cac ctc ttt ggg aaa aga ccc aat tta ctt tct tgg gac ata 1344

Asn Ser His Leu Phe Gly Lys Arg Pro Asn Leu Leu Ser Trp Asp Ile

435 440 445

agg tac aag ata ggt ctt ggt tta gcc tct gct ctg ctt tat ctt cat 1392

Arg Tyr Lys Ile Gly Leu Gly Leu Ala Ser Ala Leu Leu Tyr Leu His

450 455 460

gaa gaa tgg gat caa tgt gta ctg cac aga gac atc aag gca agt aat 1440

Glu Glu Trp Asp Gln Cys Val Leu His Arg Asp Ile Lys Ala Ser Asn

465 470 475 480

ata atg ctt gac agt gaa ttc aat gta aag tta ggt gat ttc ggg ttg 1488

Ile Met Leu Asp Ser Glu Phe Asn Val Lys Leu Gly Asp Phe Gly Leu

485 490 495

gct cgg cta atg aac cat gaa ctt ggt tcc cat aca acg ggg ttg gct 1536

Ala Arg Leu Met Asn His Glu Leu Gly Ser His Thr Thr Gly Leu Ala

500 505 510

gga act ttt ggg tac atg gca cct gaa tat gta atg aaa gga agt gcg 1584

Gly Thr Phe Gly Tyr Met Ala Pro Glu Tyr Val Met Lys Gly Ser Ala

515 520 525

agt aaa gaa tct gat ata tat agc ttt gga att gtt tta cta gag att 1632

Ser Lys Glu Ser Asp Ile Tyr Ser Phe Gly Ile Val Leu Leu Glu Ile

530 535 540

gtc aca ggg aga aaa tcg ctg gaa cgt aca caa gaa gat aac agt gat 1680

Val Thr Gly Arg Lys Ser Leu Glu Arg Thr Gln Glu Asp Asn Ser Asp

545 550 555 560

aca gag agt gat gat gag aag agt cta gtg gag aaa gtt tgg gag ctt 1728

Thr Glu Ser Asp Asp Glu Lys Ser Leu Val Glu Lys Val Trp Glu Leu

565 570 575

tat gga aaa caa gaa ctg atc act tca tgt gtt gat gac aaa ttg ggt 1776

Tyr Gly Lys Gln Glu Leu Ile Thr Ser Cys Val Asp Asp Lys Leu Gly

580 585 590

gag gat ttc gac aag aaa gaa gct gaa tgt ctt ctg gtt tta ggg tta 1824

Glu Asp Phe Asp Lys Lys Glu Ala Glu Cys Leu Leu Val Leu Gly Leu

595 600 605

tgg tgt gct cat cct gat aaa aac tcg aga cct tcg ata aaa caa ggg 1872

Trp Cys Ala His Pro Asp Lys Asn Ser Arg Pro Ser Ile Lys Gln Gly

610 615 620

atc caa gtc atg aat ttt gag tca cca ttg cct gat ctt cca ttg aag 1920

Ile Gln Val Met Asn Phe Glu Ser Pro Leu Pro Asp Leu Pro Leu Lys

625 630 635 640

agg cct gtt gct atg tat tac atc tcg act act act tct tct tct tct 1968

Arg Pro Val Ala Met Tyr Tyr Ile Ser Thr Thr Thr Ser Ser Ser Ser

645 650 655

ccc tca gtt aac tca aat gga gtt tct gta acg ttc tca ggc atc gag 2016

Pro Ser Val Asn Ser Asn Gly Val Ser Val Thr Phe Ser Gly Ile Glu

660 665 670

tat ggt cgt taa 2028

Tyr Gly Arg

675

<210>8

<211>675

<212>PRT

＜213〉Arabidopis thaliana

<400>8

Met Leu Tyr Phe Ile Phe Cys Gln Asn Leu Ser Ser Ser Ser Ser Met

1 5 10 15

Ser Asn Ser Ile Leu Phe Leu Ser Leu Phe Leu Phe Leu Pro Phe Val

20 25 30

Val Asp Ser Leu Tyr Phe Asn Phe Thr Ser Phe Arg Gln Gly Asp Pro

35 40 45

Gly Asp Ile Phe Tyr His Gly Asp Ala Thr Pro Asp Glu Asp Gly Thr

50 55 60

Val Asn Phe Asn Asn Ala Glu Gln Thr Ser Gln Val Gly Trp Ile Thr

65 70 75 80

Tyr Ser Lys Lys Val Pro Ile Trp Ser His Lys Thr Gly Lys Ala Ser

85 90 95

Asp Phe Ser Thr Ser Phe Ser Phe Lys Ile Asp Ala Arg Asn Leu Ser

100 105 110

Ala Asp Gly His Gly Ile Cys Phe Phe Leu Ala Pro Met Gly Ala Gln

115 120 125

Leu Pro Ala Tyr Ser Val Gly Gly Phe Leu Asn Leu Phe Thr Arg Lys

130 135 140

Asn Asn Tyr Ser Ser Ser Phe Pro Leu Val His Val Glu Phe Asp Thr

145 150 155 160

Phe Asn Asn Pro Gly Trp Asp Pro Asn Asp Val Gly Ser His Val Gly

165 170 175

Ile Asn Asn Asn Ser Leu Val Ser Ser Asn Tyr Thr Ser Trp Asn Ala

180 185 190

Ser Ser His Ser Gln Asp Ile Cys His Ala Lys Ile Ser Tyr Asp Ser

195 200 205

Val Thr Lys Asn Leu Ser Val Thr Trp Ala Tyr Glu Leu Thr Ala Thr

210 215 220

Ser Asp Pro Lys Glu Ser Ser Ser Leu Ser Tyr Ile Ile Asp Leu Ala

225 230 235 240

Lys Val Leu Pro Ser Asp Val Met Phe Gly Phe Ile Ala Ala Ala Gly

245 250 255

Thr Asn Thr Glu Glu His Arg Leu Leu Ser Trp Glu Leu Ser Ser Ser

260 265 270

Leu Asp Ser Asp Lys Ala Asp Ser Arg Ile Gly Leu Val Ile Gly Ile

275 280 285

Ser Ala Ser Gly Phe Val Phe Leu Thr Phe Met Val Ile Thr Thr Val

290 295 300

Val Val Trp Ser Arg Lys Gln Arg Lys Lys Lys Glu Arg Asp Ile Glu

305 310 315 320

Asn Met Ile Ser Ile Asn Lys Asp Leu Glu Arg Glu Ala Gly Pro Arg

325 330 335

Lys Phe Ser Tyr Lys Asp Leu Val Ser Ala Thr Asn Arg Phe Ser Ser

340 345 350

His Arg Lys Leu Gly Glu Gly Gly Phe Gly Ala Val Tyr Glu Gly Asn

355 360 365

Leu Lys Glu Ile Asn Thr Met Val Ala Val Lys Lys Leu Ser Gly Asp

370 375 380

Ser Arg Gln Gly Lys Asn Glu Phe Leu Asn Glu Val Lys Ile Ile Ser

385 390 395 400

Lys Leu Arg His Arg Asn Leu Val Gln Leu Ile Gly Trp Cys Asn Glu

405 410 415

Lys Asn Glu Phe Leu Leu Ile Tyr Glu Leu Val Pro Asn Gly Ser Leu

420 425 430

Asn Ser His Leu Phe Gly Lys Arg Pro Asn Leu Leu Ser Trp Asp Ile

435 440 445

Arg Tyr Lys Ile Gly Leu Gly Leu Ala Ser Ala Leu Leu Tyr Leu His

450 455 460

Glu Glu Trp Asp Gln Cys Val Leu His Arg Asp Ile Lys Ala Ser Asn

465 470 475 480

Ile Met Leu Asp Ser Glu Phe Asn Val Lys Leu Gly Asp Phe Gly Leu

485 490 495

Ala Arg Leu Met Asn His Glu Leu Gly Ser His Thr Thr Gly Leu Ala

500 505 510

Gly Thr Phe Gly Tyr Met Ala Pro Glu Tyr Val Met Lys Gly Ser Ala

515 520 525

Ser Lys Glu Ser Asp Ile Tyr Ser Phe Gly Ile Val Leu Leu Glu Ile

530 535 540

Val Thr Gly Arg Lys Ser Leu Glu Arg Thr Gln Glu Asp Asn Ser Asp

545 550 555 560

Thr Glu Ser Asp Asp Glu Lys Ser Leu Val Glu Lys Val Trp Glu Leu

565 570 575

Tyr Gly Lys Gln Glu Leu Ile Thr Ser Cys Val Asp Asp Lys Leu Gly

580 585 590

Glu Asp Phe Asp Lys Lys Glu Ala Glu Cys Leu Leu Val Leu Gly Leu

595 600 605

Trp Cys Ala His Pro Asp Lys Asn Ser Arg Pro Ser Ile Lys Gln Gly

610 615 620

Ile Gln Val Met Asn Phe Glu Ser Pro Leu Pro Asp Leu Pro Leu Lys

625 630 635 640

Arg Pro Val Ala Met Tyr Tyr Ile Ser Thr Thr Thr Ser Ser Ser Ser

645 650 655

Pro Ser Val Asn Ser Asn Gly Val Ser Val Thr Phe Ser Gly Ile Glu

660 665 670

Tyr Gly Arg

675

<210>9

<211>2694

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(2694)

<400>9

atg acg acg gcg gcg atg act tgt acg aga ctg acg ata ttt acg gtg 48

Met Thr Thr Ala Ala Met Thr Cys Thr Arg Leu Thr Ile Phe Thr Val

1 5 10 15

gcc gga ata tta ctt cag att att ggc ctc tcc att ttc gta ttc ggt 96

Ala Gly Ile Leu Leu Gln Ile Ile Gly Leu Ser Ile Phe Val Phe Gly

20 25 30

ttc ttc ccc gtc aag ccg act ctc tcc ggc gtc agt ggc tcg gag agc 144

Phe Phe Pro Val Lys Pro Thr Leu Ser Gly Val Ser Gly Ser Glu Ser

35 40 45

tat cga gat cca ttc tgt gat tct tct ctg att tcg aac gaa tcg gag 192

Tyr Arg Asp Pro Phe Cys Asp Ser Ser Leu Ile Ser Asn Glu Ser Glu

50 55 60

ctt cat cat cca gag aaa ctg aga ttg ctg tac cag gtt att gat ggg 240

Leu His His Pro Glu Lys Leu Arg Leu Leu Tyr Gln Val Ile Asp Gly

65 70 75 80

cta cct gcg gag ttc gtt ctt ggg aaa gat ggt aaa cct cca gag aag 288

Leu Pro Ala Glu Phe Val Leu Gly Lys Asp Gly Lys Pro Pro Glu Lys

85 90 95

gtt ttg aaa gag tct atg cct tat act cag tca ctg tta gct aat gga 336

Val Leu Lys Glu Ser Met Pro Tyr Thr Gln Ser Leu Leu Ala Asn Gly

100 105 110

gat gct att ggt tac cat gct aaa gca gct cct cca act gtt acc atg 384

Asp Ala Ile Gly Tyr His Ala Lys Ala Ala Pro Pro Thr Val Thr Met

115 120 125

ccg agg tta aag gca atg gtt tct ggg gca att ggt ggt ttc ttg gat 432

Pro Arg Leu Lys Ala Met Val Ser Gly Ala Ile Gly Gly Phe Leu Asp

130 135 140

gtt gct ttt aat ttt aac aca caa gct ctc tta gat gat aat att ctt 480

Val Ala Phe Asn Phe Asn Thr Gln Ala Leu Leu Asp Asp Asn Ile Leu

145 150 155 160

ggt cag ttt ttc agg att ggt tgg aaa atg gtg atg ctc ggt gat gag 528

Gly Gln Phe Phe Arg Ile Gly Trp Lys Met Val Met Leu Gly Asp Glu

165 170 175

acg tgg ctc aag tta ttt cca ggg cta ttt atg aga cat gac ggt gtt 576

Thr Trp Leu Lys Leu Phe Pro Gly Leu Phe Met Arg His Asp Gly Val

180 185 190

agc agt ttc ttt gtc aaa gat aca gta cag gta gac aga aat gtt tct 624

Ser Ser Phe Phe Val Lys Asp Thr Val Gln Val Asp Arg Asn Val Ser

195 200 205

cga cac ttg ccc gat gag cta aac agt gat gat tgg aat ctc ttg atc 672

Arg His Leu Pro Asp Glu Leu Asn Ser Asp Asp Trp Asn Leu Leu Ile

210 215 220

ctt cat tac ctt ggt tta gat cac gtt gga cat act ggc ggc cgt aac 720

Leu His Tyr Leu Gly Leu Asp His Val Gly His Thr Gly Gly Arg Asn

225 230 235 240

agc ccc ttg atg cct gca aaa ctt aaa gaa atg gat gat ata gtt aga 768

Ser Pro Leu Met Pro Ala Lys Leu Lys Glu Met Asp Asp Ile Val Arg

245 250 255

aca atg cat tta aga gcc atg atg gat cgc agc cat gat caa gga cag 816

Thr Met His Leu Arg Ala Met Met Asp Arg Ser His Asp Gln Gly Gln

260 265 270

act ctt ctg ata ata gtc agt gat cat ggc atg act gag aat gga aat 864

Thr Leu Leu Ile Ile Val Ser Asp His Gly Met Thr Glu Asn Gly Asn

275 280 285

cac gga ggg tcg tca tat gaa gaa act gac tcc tta atg ctc ttt att 912

His Gly Gly Ser Ser Tyr Glu Glu Thr Asp Ser Leu Met Leu Phe Ile

290 295 300

ggc ttg aat agc aat att tct gac tat gct tca gct acc aat aat gta 960

Gly Leu Asn Ser Asn Ile Ser Asp Tyr Ala Ser Ala Thr Asn Asn Val

305 310 315 320

gct ttc cag gta gat ttg gcg cca act tta gct ctt cta ttt ggt gtg 1008

Ala Phe Gln Val Asp Leu Ala Pro Thr Leu Ala Leu Leu Phe Gly Val

325 330 335

ccg atc cca aag aac aat gtt gga gtc ctt gtc cca gga aca ctc tgt 1056

Pro Ile Pro Lys Asn Asn Val Gly Val Leu Val Pro Gly Thr Leu Cys

340 345 350

tct tta aga gat ttt gag caa cta cgg gca cta gaa ctg aat tca tgg 1104

Ser Leu Arg Asp Phe Glu Gln Leu Arg Ala Leu Glu Leu Asn Ser Trp

355 360 365

cag tta ctc aga ctt atg cta gca cag ata caa agt tct tcg ttt cca 1152

Gln Leu Leu Arg Leu Met Leu Ala Gln Ile Gln Ser Ser Ser Phe Pro

370 375 380

cgc gtc tcc tgc aat tgc ttc ctt gat gga act tgt gag ggt ctt gat 1200

Arg Val Ser Cys Asn Cys Phe Leu Asp Gly Thr Cys Glu Gly Leu Asp

385 390 395 400

ttg gat att agt gag tgt tct ggg gat aaa gag aaa caa ctt att tgc 1248

Leu Asp Ile Ser Glu Cys Ser Gly Asp Lys Glu Lys Gln Leu Ile Cys

405 410 415

ttg ttt agg aat gct gct gcc ctc cat ggc att tgg aag tcc aag aaa 1296

Leu Phe Arg Asn Ala Ala Ala Leu His Gly Ile Trp Lys Ser Lys Lys

420 425 430

tca aca gag tcg tct agt acc atg gaa gat ttc agc aga gct tta gat 1344

Ser Thr Glu Ser Ser Ser Thr Met Glu Asp Phe Ser Arg Ala Leu Asp

435 440 445

gca tat aac acc ttc ttg aaa act gca agt gag tgg tta gca agc aaa 1392

Ala Tyr Asn Thr Phe Leu Lys Thr Ala Ser Glu Trp Leu Ala Ser Lys

450 455 460

acc acg gag aaa cct gtt ctc tta ctc ggt ctt gga gtg agt gcc atg 1440

Thr Thr Glu Lys Pro Val Leu Leu Leu Gly Leu Gly Val Ser Ala Met

465 470 475 480

ctt ctg tca tgc ttc atc tgt ggt act gtc ttt ctg tcc tta ttc aaa 1488

Leu Leu Ser Cys Phe Ile Cys Gly Thr Val Phe Leu Ser Leu Phe Lys

485 490 495

gag gtt tac cat gag ccc aag gat agg gtc tgc agt ttg agt aat ttg 1536

Glu Val Tyr His Glu Pro Lys Asp Arg Val Cys Ser Leu Ser Asn Leu

500 505 510

ttg aat tta gaa gag gtg ttc att ttt gct ctt ctt ttg ata ctt gtt 1584

Leu Asn Leu Glu Glu Val Phe Ile Phe Ala Leu Leu Leu Ile Leu Val

515 520 525

ata agc atg gga tct agt tca atg gtg gaa gaa gag cat tat ata tgg 1632

Ile Ser Met Gly Ser Ser Ser Met Val Glu Glu Glu His Tyr Ile Trp

530 535 540

cat ttc atg gta tca aca ttc cat ctt ctg tta ctc ttc aag aca gcg 1680

His Phe Met Val Ser Thr Phe His Leu Leu Leu Leu Phe Lys Thr Ala

545 550 555 560

aag tca ttc aaa att tcc aag ggg atg aac att ctc aga gac ttt aaa 1728

Lys Ser Phe Lys Ile Ser Lys Gly Met Asn Ile Leu Arg Asp Phe Lys

565 570 575

ttc ggt tct atc ttc tcg ctt ctt att tct ggt aga cta ctg aga ggt 1776

Phe Gly Ser Ile Phe Ser Leu Leu Ile Ser Gly Arg Leu Leu Arg Gly

580 585 590

tgg cat caa ggg ggc gtg aac tgg act tac ctt cct gat att tct aag 1824

Trp His Gln Gly Gly Val Asn Trp Thr Tyr Leu Pro Asp Ile Ser Lys

595 600 605

tgg cta gtg caa ggt ggc agt ggt tat gtg aaa tgg att cag cta atc 1872

Trp Leu Val Gln Gly Gly Ser Gly Tyr Val Lys Trp Ile Gln Leu Ile

610 615 620

tca ata atc cta gtt att ggt cta gga cta tat act ctc ttt cga aca 1920

Ser Ile Ile Leu Val Ile Gly Leu Gly Leu Tyr Thr Leu Phe Arg Thr

625 630 635 640

gga tcg aat aga aaa ggt gtt cgc atc cta gca ttt ggt ttc tca act 1968

Gly Ser Asn Arg Lys Gly Val Arg Ile Leu Ala Phe Gly Phe Ser Thr

645 650 655

tgt ggg ttc cta gtc ttg ctg cat gta ggg aga tat cag gat gaa ctg 2016

Cys Gly Phe Leu Val Leu Leu His Val Gly Arg Tyr Gln Asp Glu Leu

660 665 670

tca acc ggt ttt gga gcc act gtc aca gta aaa gtt att tat tat ctt 2064

Ser Thr Gly Phe Gly Ala Thr Val Thr Val Lys Val Ile Tyr Tyr Leu

675 680 685

cta tct ata tct tcc att gga gct tct ttg gtt ttg cca tgg tct gcg 2112

Leu Ser Ile Ser Ser Ile Gly Ala Ser Leu Val Leu Pro Trp Ser Ala

690 695 700

cta aac aaa gac aag tcc ttt cta gct gaa gta ggt gat tgc ttg tat 2160

Leu Asn Lys Asp Lys Ser Phe Leu Ala Glu Val Gly Asp Cys Leu Tyr

705 710 715 720

ttg att ggc tct gcg tac ata ctt tgc tgg tgt ctt cta cag caa ctt 2208

Leu Ile Gly Ser Ala Tyr Ile Leu Cys Trp Cys Leu Leu Gln Gln Leu

725 730 735

ctc caa caa cca atc aac tcg ggc ccc ata ctt ttg ctg ctc ctc caa 2256

Leu Gln Gln Pro Ile Asn Ser Gly Pro Ile Leu Leu Leu Leu Leu Gln

740 745 750

atc tta gca att tta tgt ctt tct tct agt gat ttt caa gtt aat gaa 2304

Ile Leu Ala Ile Leu Cys Leu Ser Ser Ser Asp Phe Gln Val Asn Glu

755 760 765

tgg gtc gag att act gca ctc tat tac atg gga atg gca ggt cac ttt 2352

Trp Val Glu Ile Thr Ala Leu Tyr Tyr Met Gly Met Ala Gly His Phe

770 775 780

gct ctt gga aac agc aac act tta gca aca atc gat gtt gct ggt gct 2400

Ala Leu Gly Asn Ser Asn Thr Leu Ala Thr Ile Asp Val Ala Gly Ala

785 790 795 800

ttt atc gga atc tct agt cat tct aca ata ctc tct ggg atc ttg atg 2448

Phe Ile Gly Ile Ser Ser His Ser Thr Ile Leu Ser Gly Ile Leu Met

805 810 815

ttc atg atc acc tat gca tct ccc atg ctg ttc ctt cta tcc ctc gtc 2496

Phe Met Ile Thr Tyr Ala Ser Pro Met Leu Phe Leu Leu Ser Leu Val

820 825 830

atg tac att ggt gca aat ctt aga aac cac tca aac tca aca ata tca 2544

Met Tyr Ile Gly Ala Asn Leu Arg Asn His Ser Asn Ser Thr Ile Ser

835 840 845

act cac cgg gaa acg act aaa gct cgg gtt ccc ttg cct tgt tcc gct 2592

Thr His Arg Glu Thr Thr Lys Ala Arg Val Pro Leu Pro Cys Ser Ala

850 855 860

ctg cat caa ctc aat act ctt gac cgc ata tac tgt ggt ctt act act 2640

Leu His Gln Leu Asn Thr Leu Asp Arg Ile Tyr Cys Gly Leu Thr Thr

865 870 875 880

cat gag aaa tca cct ctt tgt atg gag cgt ctt ctc tcc caa gtg agt 2688

His Glu Lys Ser Pro Leu Cys Met Glu Arg Leu Leu Ser Gln Val Ser

885 890 895

ctt tga 2694

Leu

<210>10

<211>897

<212>PRT

＜213〉Arabidopis thaliana

<400>10

Met Thr Thr Ala Ala Met Thr Cys Thr Arg Leu Thr Ile Phe Thr Val

1 5 10 15

Ala Gly Ile Leu Leu Gln Ile Ile Gly Leu Ser Ile Phe Val Phe Gly

20 25 30

Phe Phe Pro Val Lys Pro Thr Leu Ser Gly Val Ser Gly Ser Glu Ser

35 40 45

Tyr Arg Asp Pro Phe Cys Asp Ser Ser Leu Ile Ser Asn Glu Ser Glu

50 55 60

Leu His His Pro Glu Lys Leu Arg Leu Leu Tyr Gln Val Ile Asp Gly

65 70 75 80

Leu Pro Ala Glu Phe Val Leu Gly Lys Asp Gly Lys Pro Pro Glu Lys

85 90 95

Val Leu Lys Glu Ser Met Pro Tyr Thr Gln Ser Leu Leu Ala Asn Gly

100 105 110

Asp Ala Ile Gly Tyr His Ala Lys Ala Ala Pro Pro Thr Val Thr Met

115 120 125

Pro Arg Leu Lys Ala Met Val Ser Gly Ala Ile Gly Gly Phe Leu Asp

130 135 140

Val Ala Phe Asn Phe Asn Thr Gln Ala Leu Leu Asp Asp Asn Ile Leu

145 150 155 160

Gly Gln Phe Phe Arg Ile Gly Trp Lys Met Val Met Leu Gly Asp Glu

165 170 175

Thr Trp Leu Lys Leu Phe Pro Gly Leu Phe Met Arg His Asp Gly Val

180 185 190

Ser Ser Phe Phe Val Lys Asp Thr Val Gln Val Asp Arg Asn Val Ser

195 200 205

Arg His Leu Pro Asp Glu Leu Asn Ser Asp Asp Trp Asn Leu Leu Ile

210 215 220

Leu His Tyr Leu Gly Leu Asp His Val Gly His Thr Gly Gly Arg Asn

225 230 235 240

Ser Pro Leu Met Pro Ala Lys Leu Lys Glu Met Asp Asp Ile Val Arg

245 250 255

Thr Met His Leu Arg Ala Met Met Asp Arg Ser His Asp Gln Gly Gln

260 265 270

Thr Leu Leu Ile Ile Val Ser Asp His Gly Met Thr Glu Asn Gly Asn

275 280 285

His Gly Gly Ser Ser Tyr Glu Glu Thr Asp Ser Leu Met Leu Phe Ile

290 295 300

Gly Leu Asn Ser Asn Ile Ser Asp Tyr Ala Ser Ala Thr Asn Asn Val

305 310 315 320

Ala Phe Gln Val Asp Leu Ala Pro Thr Leu Ala Leu Leu Phe Gly Val

325 330 335

Pro Ile Pro Lys Asn Asn Val Gly Val Leu Val Pro Gly Thr Leu Cys

340 345 350

Ser Leu Arg Asp Phe Glu Gln Leu Arg Ala Leu Glu Leu Asn Ser Trp

355 360 365

Gln Leu Leu Arg Leu Met Leu Ala Gln Ile Gln Ser Ser Ser Phe Pro

370 375 380

Arg Val Ser Cys Asn Cys Phe Leu Asp Gly Thr Cys Glu Gly Leu Asp

385 390 395 400

Leu Asp Ile Ser Glu Cys Ser Gly Asp Lys Glu Lys Gln Leu Ile Cys

405 410 415

Leu Phe Arg Asn Ala Ala Ala Leu His Gly Ile Trp Lys Ser Lys Lys

420 425 430

Ser Thr Glu Ser Ser Ser Thr Met Glu Asp Phe Ser Arg Ala Leu Asp

435 440 445

Ala Tyr Asn Thr Phe Leu Lys Thr Ala Ser Glu Trp Leu Ala Ser Lys

450 455 460

Thr Thr Glu Lys Pro Val Leu Leu Leu Gly Leu Gly Val Ser Ala Met

465 470 475 480

Leu Leu Ser Cys Phe Ile Cys Gly Thr Val Phe Leu Ser Leu Phe Lys

485 490 495

Glu Val Tyr His Glu Pro Lys Asp Arg Val Cys Ser Leu Ser Asn Leu

500 505 5l0

Leu Asn Leu Glu Glu Val Phe Ile Phe Ala Leu Leu Leu Ile Leu Val

515 520 525

Ile Ser Met Gly Ser Ser Ser Met Val Glu Glu Glu His Tyr Ile Trp

530 535 540

His Phe Met Val Ser Thr Phe His Leu Leu Leu Leu Phe Lys Thr Ala

545 550 555 560

Lys Ser Phe Lys Ile Ser Lys Gly Met Asn Ile Leu Arg Asp Phe Lys

565 570 575

Phe Gly Ser Ile Phe Ser Leu Leu Ile Ser Gly Arg Leu Leu Arg Gly

580 585 590

Trp His Gln Gly Gly Val Asn Trp Thr Tyr Leu Pro Asp Ile Ser Lys

595 600 605

Trp Leu Val Gln Gly Gly Ser Gly Tyr Val Lys Trp Ile Gln Leu Ile

610 615 620

Ser Ile Ile Leu Val Ile Gly Leu Gly Leu Tyr Thr Leu Phe Arg Thr

625 630 635 640

Gly Ser Asn Arg Lys Gly Val Arg Ile Leu Ala Phe Gly Phe Ser Thr

645 650 655

Cys Gly Phe Leu Val Leu Leu His Val Gly Arg Tyr Gln Asp Glu Leu

660 665 670

Ser Thr Gly Phe Gly Ala Thr Val Thr Val Lys Val Ile Tyr Tyr Leu

675 680 685

Leu Ser Ile Ser Ser Ile Gly Ala Ser Leu Val Leu Pro Trp Ser Ala

690 695 700

Leu Asn Lys Asp Lys Ser Phe Leu Ala Glu Val Gly Asp Cys Leu Tyr

705 710 715 720

Leu Ile Gly Ser Ala Tyr Ile Leu Cys Trp Cys Leu Leu Gln Gln Leu

725 730 735

Leu Gln Gln Pro Ile Asn Ser Gly Pro Ile Leu Leu Leu Leu Leu Gln

740 745 750

Ile Leu Ala Ile Leu Cys Leu Ser Ser Ser Asp Phe Gln Val Asn Glu

755 760 765

Trp Val Glu Ile Thr Ala Leu Tyr Tyr Met Gly Met Ala Gly His Phe

770 775 780

Ala Leu Gly Asn Ser Asn Thr Leu Ala Thr Ile Asp Val Ala Gly Ala

785 790 795 800

Phe Ile Gly Ile Ser Ser His Ser Thr Ile Leu Ser Gly Ile Leu Met

805 810 815

Phe Met Ile Thr Tyr Ala Ser Pro Met Leu Phe Leu Leu Ser Leu Val

820 825 830

Met Tyr Ile Gly Ala Asn Leu Arg Asn His Ser Asn Ser Thr Ile Ser

835 840 845

Thr His Arg Glu Thr Thr Lys Ala Arg Val Pro Leu Pro Cys Ser Ala

850 855 860

Leu His Gln Leu Asn Thr Leu Asp Arg Ile Tyr Cys Gly Leu Thr Thr

865 870 875 880

His Glu Lys Ser Pro Leu Cys Met Glu Arg Leu Leu Ser Gln Val Ser

885 890 895

Leu

<210>11

<211>633

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(633)

<400>11

atg gcg aga gaa aag att cag atc agg aag atc gac aac gca acg gcg 48

Met Ala Arg Glu Lys Ile Gln Ile Arg Lys Ile Asp Asn Ala Thr Ala

1 5 10 15

aga caa gtg acg ttt tcg aaa cga aga aga ggg ctt ttc aag aaa gct 96

Arg Gln Val Thr Phe Ser Lys Arg Arg Arg Gly Leu Phe Lys Lys Ala

20 25 30

gaa gaa ctc tcc gtt ctc tgc gac gcc gat gtc gct ctc atc atc ttc 144

Glu Glu Leu Ser Val Leu Cys Asp Ala Asp Val Ala Leu Ile Ile Phe

35 40 45

tct tcc acc gga aaa ctg ttc gag ttc tgt agc tcc agc atg aag gaa 192

Ser Ser Thr Gly Lys Leu Phe Glu Phe Cys Ser Ser Ser Met Lys Glu

50 55 60

gtc cta gag agg cat aac ttg cag tca aag aac ttg gag aag ctt gat 240

Val Leu Glu Arg His Asn Leu Gln Ser Lys Asn Leu Glu Lys Leu Asp

65 70 75 80

cag cca tct ctt gag tta cag ctg gtt gag aac agt gat cac gcc cga 288

Gln Pro Ser Leu Glu Leu Gln Leu Val Glu Asn Ser Asp His Ala Arg

85 90 95

atg agt aaa gaa att gcg gac aag agc cac cga cta agg caa atg aga 336

Met Ser Lys Glu Ile Ala Asp Lys Ser His Arg Leu Arg Gln Met Arg

100 105 110

gga gag gaa ctt caa gga ctt gac att gaa gag ctt cag cag cta gag 384

Gly Glu Glu Leu Gln Gly Leu Asp Ile Glu Glu Leu Gln Gln Leu Glu

115 120 125

aag gcc ctt gaa act ggt ttg acg cgt gtg att gaa aca aag agt gac 432

Lys Ala Leu Glu Thr Gly Leu Thr Arg Val Ile Glu Thr Lys Ser Asp

130 135 140

aag att atg agt gag atc agc gaa ctt cag aaa aag gga atg caa ttg 480

Lys Ile Met Ser Glu Ile Ser Glu Leu Gln Lys Lys Gly Met Gln Leu

145 150 155 160

atg gat gag aac aag cgg ttg agg cag caa gta tgt gtc tta ccc tct 528

Met Asp Glu Asn Lys Arg Leu Arg Gln Gln Val Cys Val Leu Pro Ser

165 170 175

ctg ttg ata aca aat ccc ttt ctt ttg tct acc att aac gta cac acc 576

Leu Leu Ile Thr Asn Pro Phe Leu Leu Ser Thr Ile Asn Val His Thr

180 185 190

cct aaa ttt aat ccc cag ttg tct aca aca cat atg ttt gat cat act 624

Pro Lys Phe Asn Pro Gln Leu Ser Thr Thr His Met Phe Asp His Thr

195 200 205

gtg aga taa 633

Val Arg

210

<210>12

<211>210

<212>PRT

＜213〉Arabidopis thaliana

<400>12

Met Ala Arg Glu Lys Ile Gln Ile Arg Lys Ile Asp Asn Ala Thr Ala

1 5 10 15

Arg Gln Val Thr Phe Ser Lys Arg Arg Arg Gly Leu Phe Lys Lys Ala

20 25 30

Glu Glu Leu Ser Val Leu Cys Asp Ala Asp Val Ala Leu Ile Ile Phe

35 40 45

Ser Ser Thr Gly Lys Leu Phe Glu Phe Cys Ser Ser Ser Met Lys Glu

50 55 60

Val Leu Glu Arg His Asn Leu Gln Ser Lys Asn Leu Glu Lys Leu Asp

65 70 75 80

Gln Pro Ser Leu Glu Leu Gln Leu Val Glu Asn Ser Asp His Ala Arg

85 90 95

Met Ser Lys Glu Ile Ala Asp Lys Ser His Arg Leu Arg Gln Met Arg

100 105 110

Gly Glu Glu Leu Gln Gly Leu Asp Ile Glu Glu Leu Gln Gln Leu Glu

115 120 125

Lys Ala Leu Glu Thr Gly Leu Thr Arg Val Ile Glu Thr Lys Ser Asp

130 135 140

Lys Ile Met Ser Glu Ile Ser Glu Leu Gln Lys Lys Gly Met Gln Leu

145 150 155 160

Met Asp Glu Asn Lys Arg Leu Arg Gln Gln Val Cys Val Leu Pro Ser

165 170 175

Leu Leu Ile Thr Asn Pro Phe Leu Leu Ser Thr Ile Asn Val His Thr

180 185 190

Pro Lys Phe Asn Pro Gln Leu Ser Thr Thr His Met Phe Asp His Thr

195 200 205

Val Arg

210

<210>13

<211>1215

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(1215)

<400>13

atg gaa cca atc cca aat gat ctc att ctc gag atc ttc tcg aga ttg 48

Met Glu Pro Ile Pro Asn Asp Leu Ile Leu Glu Ile Phe Ser Arg Leu

1 5 10 15

ccg gcg aag tca gtc att gga ttt cgt acc ttg tcg aag cac tgg gcg 96

Pro Ala Lys Ser Val Ile Gly Phe Arg Thr Leu Ser Lys His Trp Ala

20 25 30

tct att ctt cgc agt cca gtt ttc acc gag ctc ttc ctg acc aga tcc 144

Ser Ile Leu Arg Ser Pro Val Phe Thr Glu Leu Phe Leu Thr Arg Ser

35 40 45

tcg aat cgt cca cgt ctg ctg ttt gct gcc gaa cga aat ggt gag tgg 192

Ser Asn Arg Pro Arg Leu Leu Phe Ala Ala Glu Arg Asn Gly Glu Trp

50 55 60

ctc ttc ttc tcg tcg ccg cag cca cag aat cga tat gag aag tct tct 240

Leu Phe Phe Ser Ser Pro Gln Pro Gln Asn Arg Tyr Glu Lys Ser Ser

65 70 75 80

cat ttg gat tat cat aca aaa ttc tca gga gac gta agc cga ttc att 288

His Leu Asp Tyr His Thr Lys Phe Ser Gly Asp Val Ser Arg Phe Ile

85 90 95

tgt agc tat gtc tca ggt ttg ctc tgt ttc cct gat ttg tgg ctc tca 336

Cys Ser Tyr Val Ser Gly Leu Leu Cys Phe Pro Asp Leu Trp Leu Ser

100 105 110

aaa gat gca tcg cct gtg att tgt aac cct acc aca gga atg tat gag 384

Lys Asp Ala Ser Pro Val Ile Cys Asn Pro Thr Thr Gly Met Tyr Glu

115 120 125

agc tta cct gat ctg atg agg tac aaa aat gct cga ggc ttt cta ggg 432

Ser Leu Pro Asp Leu Met Arg Tyr Lys Asn Ala Arg Gly Phe Leu Gly

130 135 140

ttt gat ccc att ggt aag caa ttc aag gta ttg tct gag gct tat cca 480

Phe Asp Pro Ile Gly Lys Gln Phe Lys Val Leu Ser Glu Ala Tyr Pro

145 150 155 160

ttt tct gat caa agg gaa cat cat gag att ctg aca tta gga act gag 528

Phe Ser Asp Gln Arg Glu His His Glu Ile Leu Thr Leu Gly Thr Glu

165 170 175

gaa ctg agc tgg agg agt aat atc att agt tgt cct gca tat gat cgt 576

Glu Leu Ser Trp Arg Ser Asn Ile Ile Ser Cys Pro Ala Tyr Asp Arg

180 185 190

tct ttg agt gaa gga ata tgt atc aat gga gtt ttg tat tac tta gct 624

Ser Leu Ser Glu Gly Ile Cys Ile Asn Gly Val Leu Tyr Tyr Leu Ala

195 200 205

cag act ctt ggt gta ccg tct tgt gtg atc att tgc ttt gat gta agg 672

Gln Thr Leu Gly Val Pro Ser Cys Val Ile Ile Cys Phe Asp Val Arg

210 215 220

tct gag gaa ttt aag tat ttt gac gca gga tgc ttt aat gat caa ctt 720

Ser Glu Glu Phe Lys Tyr Phe Asp Ala Gly Cys Phe Asn Asp Gln Leu

225 230 235 240

gat gat act agt ggg ttg att ttg gta aac tat gag ggt aaa tta agt 768

Asp Asp Thr Ser Gly Leu Ile Leu Val Asn Tyr Glu Gly Lys Leu Ser

245 250 255

ggg att aat tgg aag tat ggt caa gct ggt gaa agg cgc acc gtt gag 816

Gly Ile Asn Trp Lys Tyr Gly Gln Ala Gly Glu Arg Arg Thr Val Glu

260 265 270

ttg cgt atg tgg gtt cta gag gat gcc gag aag cat gaa tgg gtt aaa 864

Leu Arg Met Trp Val Leu Glu Asp Ala Glu Lys His Glu Trp Val Lys

275 280 285

tat gtg tac act ctg ccg gag aat gaa gtc ctt gat tcc tgc gac ttt 912

Tyr Val Tyr Thr Leu Pro Glu Asn Glu Val Leu Asp Ser Cys Asp Phe

290 295 300

tca gtt gct gga gtg act act aga ggt gat att gtt ttg tgt atg aaa 960

Ser Val Ala Gly Val Thr Thr Arg Gly Asp Ile Val Leu Cys Met Lys

305 310 315 320

tat aca tgt aaa ccg ttt tat gtg ttc tac ttc aat ccc gag agg aac 1008

Tyr Thr Cys Lys Pro Phe Tyr Val Phe Tyr Phe Asn Pro Glu Arg Asn

325 330 335

act ctc cag agc gtt gaa atc caa gat ttt gga gct aac ctt gaa gcg 1056

Thr Leu Gln Ser Val Glu Ile Gln Asp Phe Gly Ala Asn Leu Glu Ala

340 345 350

gtt gag aat tgt ggt aga gtt tat gct ttt gtt aac cat gta gag gat 1104

Val Glu Asn Cys Gly Arg Val Tyr Ala Phe Val Asn His Val Glu Asp

355 360 365

ctt cgg gtt aac aaa ggg aag caa ctc aag tca agc atc tct caa gtc 1152

Leu Arg Val Asn Lys Gly Lys Gln Leu Lys Ser Ser Ile Ser Gln Val

370 375 380

aag cat ctc tgc tca tgc tgt aac aaa gta tcg cag cct gat tat cat 1200

Lys His Leu Cys Ser Cys Cys Asn Lys Val Ser Gln Pro Asp Tyr His

385 390 395 400

tac caa aag gct taa 1215

Tyr Gln Lys Ala

<210>14

<211>404

<212>PRT

＜213〉Arabidopis thaliana

<400>14

Met Glu Pro Ile Pro Asn Asp Leu Ile Leu Glu Ile Phe Ser Arg Leu

1 5 10 15

Pro Ala Lys Ser Val Ile Gly Phe Arg Thr Leu Ser Lys His Trp Ala

20 25 30

Ser Ile Leu Arg Ser Pro Val Phe Thr Glu Leu Phe Leu Thr Arg Ser

35 40 45

Ser Asn Arg Pro Arg Leu Leu Phe Ala Ala Glu Arg Asn Gly Glu Trp

50 55 60

Leu Phe Phe Ser Ser Pro Gln Pro Gln Asn Arg Tyr Glu Lys Ser Ser

65 70 75 80

His Leu Asp Tyr His Thr Lys Phe Ser Gly Asp Val Ser Arg Phe Ile

85 90 95

Cys Ser Tyr Val Ser Gly Leu Leu Cys Phe Pro Asp Leu Trp Leu Ser

100 105 110

Lys Asp Ala Ser Pro Val Ile Cys Asn Pro Thr Thr Gly Met Tyr Glu

115 120 125

Ser Leu Pro Asp Leu Met Arg Tyr Lys Asn Ala Arg Gly Phe Leu Gly

130 135 140

Phe Asp Pro Ile Gly Lys Gln Phe Lys Val Leu Ser Glu Ala Tyr Pro

145 150 155 160

Phe Ser Asp Gln Arg Glu His His Glu Ile Leu Thr Leu Gly Thr Glu

165 170 175

Glu Leu Ser Trp Arg Ser Asn Ile Ile Ser Cys Pro Ala Tyr Asp Arg

180 185 190

Ser Leu Ser Glu Gly Ile Cys Ile Asn Gly Val Leu Tyr Tyr Leu Ala

195 200 205

Gln Thr Leu Gly Val Pro Ser Cys Val Ile Ile Cys Phe Asp Val Arg

210 215 220

Ser Glu Glu Phe Lys Tyr Phe Asp Ala Gly Cys Phe Asn Asp Gln Leu

225 230 235 240

Asp Asp Thr Ser Gly Leu Ile Leu Val Asn Tyr Glu Gly Lys Leu Ser

245 250 255

Gly Ile Asn Trp Lys Tyr Gly Gln Ala Gly Glu Arg Arg Thr Val Glu

260 265 270

Leu Arg Met Trp Val Leu Glu Asp Ala Glu Lys His Glu Trp Val Lys

275 280 285

Tyr Val Tyr Thr Leu Pro Glu Asn Glu Val Leu Asp Ser Cys Asp Phe

290 295 300

Ser Val Ala Gly Val Thr Thr Arg Gly Asp Ile Val Leu Cys Met Lys

305 310 315 320

Tyr Thr Cys Lys Pro Phe Tyr Val Phe Tyr Phe Asn Pro Glu Arg Asn

325 330 335

Thr Leu Gln Ser Val Glu Ile Gln Asp Phe Gly Ala Asn Leu Glu Ala

340 345 350

Val Glu Asn Cys Gly Arg Val Tyr Ala Phe Val Asn His Val Glu Asp

355 360 365

Leu Arg Val Asn Lys Gly Lys Gln Leu Lys Ser Ser Ile Ser Gln Val

370 375 380

Lys His Leu Cys Ser Cys Cys Asn Lys Val Ser Gln Pro Asp Tyr His

385 390 395 400

Tyr Gln Lys Ala

<210>15

<211>1767

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(1767)

<400>15

atg gca gta gga aag gtt gtg gta tct gtg gca tca ctc ctc cta gtg 48

Met Ala Val Gly Lys Val Val Val Ser Val Ala Ser Leu Leu Leu Val

1 5 10 15

gtg ggt gtt gcc ata gga gtt atc acg ttc gtt aat aaa gga ggc ggc 96

Val Gly Val Ala Ile Gly Val Ile Thr Phe Val Asn Lys Gly Gly Gly

20 25 30

gcc aat ggc gac agt aat ggt ccc ata aac tct cat cag aaa gcg gtt 144

Ala Asn Gly Asp Ser Asn Gly Pro Ile Asn Ser His Gln Lys Ala Val

35 40 45

cag aca att tgc cag tca acc aca gac caa ggt tcg tgc gcg aaa aca 192

Gln Thr Ile Cys Gln Ser Thr Thr Asp Gln Gly Ser Cys Ala Lys Thr

50 55 60

ctc gac cca gtc aaa agt gat gat cca agc aag ctt gtc aaa gcc ttc 240

Leu Asp Pro Val Lys Ser Asp Asp Pro Ser Lys Leu Val Lys Ala Phe

65 70 75 80

ctg atg gct act aaa gac gcg atc aca aaa tcc tca aac ttc acg gct 288

Leu Met Ala Thr Lys Asp Ala Ile Thr Lys Ser Ser Asn Phe Thr Ala

85 90 95

tcg acc gaa gga ggt atg ggt act aac atg aac gcg acc agc aaa gcc 336

Ser Thr Glu Gly Gly Met Gly Thr Asn Met Asn Ala Thr Ser Lys Ala

100 105 110

gtt ctt gat tac tgc aag aga gta ttg atg tac gct ctt gag gat ctt 384

Val Leu Asp Tyr Cys Lys Arg Val Leu Met Tyr Ala Leu Glu Asp Leu

115 120 125

gag acc att gtt gaa gag atg ggt gag gat ctt cag cag agt gga act 432

Glu Thr Ile Val Glu Glu Met Gly Glu Asp Leu Gln Gln Ser Gly Thr

130 135 140

aag ctt gac cag ctc aaa caa tgg tta act gga gtt ttc aac tac caa 480

Lys Leu Asp Gln Leu Lys Gln Trp Leu Thr Gly Val Phe Asn Tyr Gln

145 150 155 160

acc gat tgt ctt gac gac atc gag gaa gtt gag cta aag aag atc atg 528

Thr Asp Cys Leu Asp Asp Ile Glu Glu Val Glu Leu Lys Lys Ile Met

165 170 175

ggt gaa gga atc tct aac tcc aag gtt ttg acc agt aac gct atc gat 576

Gly Glu Gly Ile Ser Asn Ser Lys Val Leu Thr Ser Asn Ala Ile Asp

180 185 190

atc ttc cat tcc gtt gtt acc gcg atg gcc caa atg ggt gtc aaa gtc 624

Ile Phe His Ser Val Val Thr Ala Met Ala Gln Met Gly Val Lys Val

195 200 205

gac gat atg aag aac ata acc atg gga gcc gga gcc ggt gga gct gca 672

Asp Asp Met Lys Asn Ile Thr Met Gly Ala Gly Ala Gly Gly Ala Ala

210 215 220

cgt cgc ctt ctt gaa gac aac gac tct aag gga ctc ccc aaa tgg ttt 720

Arg Arg Leu Leu Glu Asp Asn Asp Ser Lys Gly Leu Pro Lys Trp Phe

225 230 235 240

tct ggt aaa gac agg aag ctt atg gct aag gcc gga cgt ggc gct cca 768

Ser Gly Lys Asp Arg Lys Leu Met Ala Lys Ala Gly Arg Gly Ala Pro

245 250 255

gct ggc ggt gat gat ggt atc ggt gaa ggc ggt ggt ggt ggc ggt aag 816

Ala Gly Gly Asp Asp Gly Ile Gly Glu Gly Gly Gly Gly Gly Gly Lys

260 265 270

atc aag gcg act cat gta gtg gct aag gat gga agt gga cag ttt aag 864

Ile Lys Ala Thr His Val Val Ala Lys Asp Gly Ser Gly Gln Phe Lys

275 280 285

acc att tct gag gcg gtt atg gct tgc ccg gat aaa aac cct gga agg 912

Thr Ile Ser Glu Ala Val Met Ala Cys Pro Asp Lys Asn Pro Gly Arg

290 295 300

tgc att atc cat atc aag gct ggt atc tac aat gaa caa gtc aga atc 960

Cys Ile Ile His Ile Lys Ala Gly Ile Tyr Asn Glu Gln Val Arg Ile

305 310 315 320

cct aag aag aag aac aac att ttc atg ttc ggt gat ggt gct aca caa 1008

Pro Lys Lys Lys Asn Asn Ile Phe Met Phe Gly Asp Gly Ala Thr Gln

325 330 335

acc atc att acc ttt gac aga agt gtt aaa ctt agc cca gga acc act 1056

Thr Ile Ile Thr Phe Asp Arg Ser Val Lys Leu Ser Pro Gly Thr Thr

340 345 350

act tca ctc agt ggc acc gtt cag gtc gaa tct gag gga ttc atg gcg 1104

Thr Ser Leu Ser Gly Thr Val Gln Val Glu Ser Glu Gly Phe Met Ala

355 360 365

aaa tgg att ggg ttt aag aac act gct ggt cca ttg gga cac caa gcg 1152

Lys Trp Ile Gly Phe Lys Asn Thr Ala Gly Pro Leu Gly His Gln Ala

370 375 380

gtc gca ctc cgt gtg aac gga gac cgt gcg gtc ata ttc aac tgt aga 1200

Val Ala Leu Arg Val Asn Gly Asp Arg Ala Val Ile Phe Ash Cys Arg

385 390 395 400

ttc gac ggt tac caa gac aca ctc tac gtc aac aac gga cgt caa ttc 1248

Phe Asp Gly Tyr Gln Asp Thr Leu Tyr Val Asn Asn Gly Arg Gln Phe

405 410 415

tac agg aac att gtt gta tcc ggt aca gtg gat ttc atc ttc gga aaa 1296

Tyr Arg Asn Ile Val Val Ser Gly Thr Val Asp Phe Ile Phe Gly Lys

420 425 430

tcc gca act gtg atc caa aac tct ctc atc ctc gtc cga aag gga agc 1344

Ser Ala Thr Val Ile Gln Asn Ser Leu Ile Leu Val Arg Lys Gly Ser

435 440 445

ccc gga caa tcc aac tac gtt acg gct gac ggt aat gaa aag ggt gca 1392

Pro Gly Gln Ser Asn Tyr Val Thr Ala Asp Gly Asn Glu Lys Gly Ala

450 455 460

gcg atg aag atc ggt atc gtc ctc cat aac tgc cgt atc ata ccg gac 1440

Ala Met Lys Ile Gly Ile Val Leu His Asn Cys Arg Ile Ile Pro Asp

465 470 475 480

aag gag ctc gaa gct gac aag cta acc atc aaa tcg tac cta gga agg 1488

Lys Glu Leu Glu Ala Asp Lys Leu Thr Ile Lys Ser Tyr Leu Gly Arg

485 490 495

ccg tgg aag aag ttt gcc acg act gtg att att gga act gag att ggt 1536

Pro Trp Lys Lys Phe Ala Thr Thr Val Ile Ile Gly Thr Glu Ile Gly

500 505 510

gat ttg att aaa cca gaa gga tgg acc gaa tgg caa gga gaa caa aac 1584

Asp Leu Ile Lys Pro Glu Gly Trp Thr Glu Trp Gln Gly Glu Gln Asn

515 520 525

cac aag act gct aaa tac att gag ttc aat aac cgt gga ccg gga gct 1632

His Lys Thr Ala Lys Tyr Ile Glu Phe Asn Asn Arg Gly Pro Gly Ala

530 535 540

gcc act act cag agg cct cct tgg gtt aag gtg gct aag tct gcg gct 1680

Ala Thr Thr Gln Arg Pro Pro Trp Val Lys Val Ala Lys Ser Ala Ala

545 550 555 560

gag gtt gaa act tac acc gtg gct aac tgg gtt ggt cca gct aac tgg 1728

Glu Val Glu Thr Tyr Thr Val Ala Asn Trp Val Gly Pro Ala Asn Trp

565 570 575

atc caa gaa gcc aac gtg ccc gtc caa cta gga ttg taa 1767

Ile Gln Glu Ala Asn Val Pro Val Gln Leu Gly Leu

580 585

<210>16

<211>588

<212>PRT

＜213〉Arabidopis thaliana

<400>16

Met Ala Val Gly Lys Val Val Val Ser Val Ala Ser Leu Leu Leu Val

1 5 10 15

Val Gly Val Ala Ile Gly Val Ile Thr Phe Val Asn Lys Gly Gly Gly

20 25 30

Ala Asn Gly Asp Ser Asn Gly Pro Ile Asn Ser His Gln Lys Ala Val

35 40 45

Gln Thr Ile Cys Gln Ser Thr Thr Asp Gln Gly Ser Cys Ala Lys Thr

50 55 60

Leu Asp Pro Val Lys Ser Asp Asp Pro Ser Lys Leu Val Lys Ala Phe

65 70 75 80

Leu Met Ala Thr Lys Asp Ala Ile Thr Lys Ser Ser Asn Phe Thr Ala

85 90 95

Ser Thr Glu Gly Gly Met Gly Thr Asn Met Asn Ala Thr Ser Lys Ala

100 105 110

Val Leu Asp Tyr Cys Lys Arg Val Leu Met Tyr Ala Leu Glu Asp Leu

115 120 125

Glu Thr Ile Val Glu Glu Met Gly Glu Asp Leu Gln Gln Ser Gly Thr

130 135 140

Lys Leu Asp Gln Leu Lys Gln Trp Leu Thr Gly Val Phe Asn Tyr Gln

145 150 155 160

Thr Asp Cys Leu Asp Asp Ile Glu Glu Val Glu Leu Lys Lys Ile Met

165 170 175

Gly Glu Gly Ile Ser Asn Ser Lys Val Leu Thr Ser Asn Ala Ile Asp

180 185 190

Ile Phe His Ser Val Val Thr Ala Met Ala Gln Met Gly Val Lys Val

195 200 205

Asp Asp Met Lys Asn Ile Thr Met Gly Ala Gly Ala Gly Gly Ala Ala

210 215 220

Arg Arg Leu Leu Glu Asp Asn Asp Ser Lys Gly Leu Pro Lys Trp Phe

225 230 235 240

Ser Gly Lys Asp Arg Lys Leu Met Ala Lys Ala Gly Arg Gly Ala Pro

245 250 255

Ala Gly Gly Asp Asp Gly Ile Gly Glu Gly Gly Gly Gly Gly Gly Lys

260 265 270

Ile Lys Ala Thr His Val Val Ala Lys Asp Gly Ser Gly Gln Phe Lys

275 280 285

Thr Ile Ser Glu Ala Val Met Ala Cys Pro Asp Lys Asn Pro Gly Arg

290 295 300

Cys Ile Ile His Ile Lys Ala Gly Ile Tyr Asn Glu Gln Val Arg Ile

305 310 315 320

Pro Lys Lys Lys Asn Asn Ile Phe Met Phe Gly Asp Gly Ala Thr Gln

325 330 335

Thr Ile Ile Thr Phe Asp Arg Ser Val Lys Leu Ser Pro Gly Thr Thr

340 345 350

Thr Ser Leu Ser Gly Thr Val Gln Val Glu Ser Glu Gly Phe Met Ala

355 360 365

Lys Trp Ile Gly Phe Lys Asn Thr Ala Gly Pro Leu Gly His Gln Ala

370 375 380

Val Ala Leu Arg Val Asn Gly Asp Arg Ala Val Ile Phe Asn Cys Arg

385 390 395 400

Phe Asp Gly Tyr Gln Asp Thr Leu Tyr Val Asn Asn Gly Arg Gln Phe

405 410 415

Tyr Arg Asn Ile Val Val Ser Gly Thr Val Asp Phe Ile Phe Gly Lys

420 425 430

Ser Ala Thr Val Ile Gln Asn Ser Leu Ile Leu Val Arg Lys Gly Ser

435 440 445

Pro Gly Gln Ser Asn Tyr Val Thr Ala Asp Gly Asn Glu Lys Gly Ala

450 455 460

Ala Met Lys Ile Gly Ile Val Leu His Asn Cys Arg Ile Ile Pro Asp

465 470 475 480

Lys Glu Leu Glu Ala Asp Lys Leu Thr Ile Lys Ser Tyr Leu Gly Arg

485 490 495

Pro Trp Lys Lys Phe Ala Thr Thr Val Ile Ile Gly Thr Glu Ile Gly

500 505 510

Asp Leu Ile Lys Pro Glu Gly Trp Thr Glu Trp Gln Gly Glu Gln Asn

515 520 525

His Lys Thr Ala Lys Tyr Ile Glu Phe Asn Asn Arg Gly Pro Gly Ala

530 535 540

Ala Thr Thr Gln Arg Pro Pro Trp Val Lys Val Ala Lys Ser Ala Ala

545 550 555 560

Glu Val Glu Thr Tyr Thr Val Ala Asn Trp Val Gly Pro Ala Asn Trp

565 570 575

Ile Gln Glu Ala Asn Val Pro Val Gln Leu Gly Leu

580 585

<210>17

<211>648

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(648)

<400>17

atg aac act cca atc aaa ctc ttc ttt ctc ttc ata ttc gtt gcc gca 48

Met Asn Thr Pro Ile Lys Leu Phe Phe Leu Phe Ile Phe Val Ala Ala

1 5 10 15

acc gca acg gct aaa cca gtc aaa cga acg aac tac ctt ttc aca ccg 96

Thr Ala Thr Ala Lys Pro Val Lys Arg Thr Asn Tyr Leu Phe Thr Pro

20 25 30

cac gcc aaa gca gtc gcg gga atc tgc acc gtt atc cca acg gac aca 144

His Ala Lys Ala Val Ala Gly Ile Cys Thr Val Ile Pro Thr Asp Thr

35 40 45

agt cta tgt tgc aaa acc ctt aaa cac gtt cct acc aat gac ccc atc 192

Ser Leu Cys Cys Lys Thr Leu Lys His Val Pro Thr Asn Asp Pro Ile

50 55 60

gaa cta atc cga gca tta gtg gct gcc gct gaa aca tcc gtc aaa caa 240

Glu Leu Ile Arg Ala Leu Val Ala Ala Ala Glu Thr Ser Val Lys Gln

65 70 75 80

agt gtg act ttc ctc tct gga atc aaa cca aaa cac atg tca gac gca 288

Ser Val Thr Phe Leu Ser Gly Ile Lys Pro Lys His Met Ser Asp Ala

85 90 95

acc gca acc gca gtg gtc aac agc tgc gag aaa aac ttg aat tac gcg 336

Thr Ala Thr Ala Val Val Asn Ser Cys Glu Lys Asn Leu Asn Tyr Ala

100 105 110

ttg gaa gat ttc gcc gat ttt tgg aaa gct acg ggg aaa gat gta acg 384

Leu Glu Asp Phe Ala Asp Phe Trp Lys Ala Thr Gly Lys Asp Val Thr

115 120 125

acg ttg gct cat aat tat ttt acg tgt aaa aaa gag tta atg tca atc 432

Thr Leu Ala His Asn Tyr Phe Thr Cys Lys Lys Glu Leu Met Ser Ile

130 135 140

atg ggg tat cat tcg act tgt ttg gac gat att gaa gac aag att ttg 480

Met Gly Tyr His Ser Thr Cys Leu Asp Asp Ile Glu Asp Lys Ile Leu

145 150 155 160

ttg aag gaa gtg ggg att ggg att gga gtt ggg aag aat cta aca agt 528

Leu Lys Glu Val Gly Ile Gly Ile Gly Val Gly Lys Asn Leu Thr Ser

165 170 175

gat tct ttt gat gtg ttt aat aac ttg aat act att ttc aag act ttt 576

Asp Ser Phe Asp Val Phe Asn Asn Leu Asn Thr Ile Phe Lys Thr Phe

180 185 190

ggt att aag gtg aag ctt aac gag gaa gac act tcg ccc cga ccg cca 624

Gly Ile Lys Val Lys Leu Asn Glu Glu Asp Thr Ser Pro Arg Pro Pro

195 200 205

ccc ttg tct gat tat tat tac tga 648

Pro Leu Ser Asp Tyr Tyr Tyr

210 215

<210>18

<211>215

<212>PRT

＜213〉Arabidopis thaliana

<400>18

Met Asn Thr Pro Ile Lys Leu Phe Phe Leu Phe Ile Phe Val Ala Ala

1 5 10 15

Thr Ala Thr Ala Lys Pro Val Lys Arg Thr Asn Tyr Leu Phe Thr Pro

20 25 30

His Ala Lys Ala Val Ala Gly Ile Cys Thr Val Ile Pro Thr Asp Thr

35 40 45

Ser Leu Cys Cys Lys Thr Leu Lys His Val Pro Thr Asn Asp Pro Ile

50 55 60

Glu Leu Ile Arg Ala Leu Val Ala Ala Ala Glu Thr Ser Val Lys Gln

65 70 75 80

Ser Val Thr Phe Leu Ser Gly Ile Lys Pro Lys His Met Ser Asp Ala

85 90 95

Thr Ala Thr Ala Val Val Asn Ser Cys Glu Lys Asn Leu Asn Tyr Ala

100 105 110

Leu Glu Asp Phe Ala Asp Phe Trp Lys Ala Thr Gly Lys Asp Val Thr

115 120 125

Thr Leu Ala His Asn Tyr Phe Thr Cys Lys Lys Glu Leu Met Ser Ile

130 135 140

Met Gly Tyr His Ser Thr Cys Leu Asp Asp Ile Glu Asp Lys Ile Leu

145 150 155 160

Leu Lys Glu Val Gly Ile Gly Ile Gly Val Gly Lys Asn Leu Thr Ser

165 170 175

Asp Ser Phe Asp Val Phe Asn Asn Leu Asn Thr Ile Phe Lys Thr Phe

180 185 190

Gly Ile Lys Val Lys Leu Asn Glu Glu Asp Thr Ser Pro Arg Pro Pro

195 200 205

Pro Leu Ser Asp Tyr Tyr Tyr

210 215

<210>19

<211>408

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(408)

<400>19

atg aga ttg ctt caa gaa ctt gtt ccc gga tgc aac aag atc acc gga 48

Met Arg Leu Leu Gln Glu Leu Val Pro Gly Cys Asn Lys Ile Thr Gly

1 5 10 15

aaa gcg gtt atg ctc gat gaa ata atc aac tat gtt cag tca ttg caa 96

Lys Ala Val Met Leu Asp Glu Ile Ile Asn Tyr Val Gln Ser Leu Gln

20 25 30

cag caa gtt gag ttt ttg tct atg aaa ctt gcg acg gtg aat cca gag 144

Gln Gln Val Glu Phe Leu Ser Met Lys Leu Ala Thr Val Asn Pro Glu

35 40 45

atc aat att gat ata gac agg att ctc gcc aaa gat ctt ctg cag tca 192

Ile Asn Ile Asp Ile Asp Arg Ile Leu Ala Lys Asp Leu Leu Gln Ser

50 55 60

aga gac aga aac act cct aca ctc ggg ctg aat cct ttt gcc ggt ttt 240

Arg Asp Arg Asn Thr Pro Thr Leu Gly Leu Asn Pro Phe Ala Gly Phe

65 70 75 80

caa ggg aac ata cca aac ctt tct gcc acc aca aat cca caa tac aac 288

Gln Gly Asn Ile Pro Asn Leu Ser Ala Thr Thr Asn Pro Gln Tyr Asn

85 90 95

cca cta cct cag aca aca cta gag agt gaa cta caa aac ctt tac cag 336

Pro Leu Pro Gln Thr Thr Leu Glu Ser Glu Leu Gln Asn Leu Tyr Gln

100 105 110

atg gga ttc gtc tca aat cca tcg act atg tcc agt ttc tca cct aat 384

Met Gly Phe Val Ser Asn Pro Ser Thr Met Ser Ser Phe Ser Pro Asn

115 120 125

ggt cga ttg aaa cct gag ctc tag 408

Gly Arg Leu Lys Pro Glu Leu

130 135

<210>20

<211>135

<212>PRT

＜213〉Arabidopis thaliana

<400>20

Met Arg Leu Leu Gln Glu Leu Val Pro Gly Cys Asn Lys Ile Thr Gly

1 5 10 15

Lys Ala Val Met Leu Asp Glu Ile Ile Asn Tyr Val Gln Ser Leu Gln

20 25 30

Gln Gln Val Glu Phe Leu Ser Met Lys Leu Ala Thr Val Asn Pro Glu

35 40 45

Ile Asn Ile Asp Ile Asp Arg Ile Leu Ala Lys Asp Leu Leu Gln Ser

50 55 60

Arg Asp Arg Asn Thr Pro Thr Leu Gly Leu Asn Pro Phe Ala Gly Phe

65 70 75 80

Gln Gly Asn Ile Pro Asn Leu Ser Ala Thr Thr Asn Pro Gln Tyr Asn

85 90 95

Pro Leu Pro Gln Thr Thr Leu Glu Ser Glu Leu Gln Asn Leu Tyr Gln

100 105 110

Met Gly Phe Val Ser Asn Pro Ser Thr Met Ser Ser Phe Ser Pro Asn

115 120 125

Gly Arg Leu Lys Pro Glu Leu

130 135

<210>21

<211>2979

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(2979)

<400>21

atg gaa gac gcc tac gcc aga tct gtc tca gag gtg ctt gat ttc ttt 48

Met Glu Asp Ala Tyr Ala Arg Ser Val Ser Glu Val Leu Asp Phe Phe

1 5 10 15

ggg gta gac cca aca aag ggt ctt tct gat tct cag gtt gtt cat cat 96

Gly Val Asp Pro Thr Lys Gly Leu Ser Asp Ser Gln Val Val His His

20 25 30

tcc agg ctt tat ggc agg aat gga acg cca ttc tgg aaa ctg gtt ctg 144

Ser Arg Leu Tyr Gly Arg Asn Gly Thr Pro Phe Trp Lys Leu Val Leu

35 40 45

aaa cag ttt gat gat tta ctt gtc aag ata ttg att gtg gct gca att 192

Lys Gln Phe Asp Asp Leu Leu Val Lys Ile Leu Ile Val Ala Ala Ile

50 55 60

gtt tct ttc gta ttg gct ttg gct aat gga gag act ggt tta aca gca 240

Val Ser Phe Val Leu Ala Leu Ala Asn Gly Glu Thr Gly Leu Thr Ala

65 70 75 80

ttt ctg gag cct ttt gtc att ctg ctg ata ttg gct gca aat gcg gca 288

Phe Leu Glu Pro Phe Val Ile Leu Leu Ile Leu Ala Ala Asn Ala Ala

85 90 95

gtg ggg gtg atc acg gag act aat gct gag aag gct ctt gag gag cta 336

Val Gly Val Ile Thr Glu Thr Asn Ala Glu Lys Ala Leu Glu Glu Leu

100 105 110

cgt gcc tac caa gca aat ata gct aca gtg ttg cga aat ggg tgc ttc 384

Arg Ala Tyr Gln Ala Asn Ile Ala Thr Val Leu Arg Asn Gly Cys Phe

115 120 125

tct atc cta cca gca aca gag ctg gtt cca ggc gac att gtt gaa gtt 432

Ser Ile Leu Pro Ala Thr Glu Leu Val Pro Gly Asp Ile Val Glu Val

130 135 140

act gtg gga tgt aag att cca gct gac ctg agg atg att gag atg tct 480

Thr Val Gly Cys Lys Ile Pro Ala Asp Leu Arg Met Ile Glu Met Ser

145 150 155 160

agc aat acg ttt cga gtt gat caa gcc att cta act ggt gaa agc tgt 528

Ser Asn Thr Phe Arg Val Asp Gln Ala Ile Leu Thr Gly Glu Ser Cys

165 170 175

tcc gtg gaa aaa gat gtt gac tgt act tta aca aca aat gct gtc tac 576

Ser Val Glu Lys Asp Val Asp Cys Thr Leu Thr Thr Asn Ala Val Tyr

180 185 190

caa gac aag aaa aat att tta ttt tcg gga act gat gtg gtc gcg ggt 624

Gln Asp Lys Lys Asn Ile Leu Phe Ser Gly Thr Asp Val Val Ala Gly

195 200 205

agg gga agg gct gtt gtc att gga gtt ggt tca aac acc gca atg ggt 672

Arg Gly Arg Ala Val Val Ile Gly Val Gly Ser Asn Thr Ala Met Gly

210 215 220

agc ata cac gat tct atg ttg cag aca gat gat gag gca act cca ttg 720

Ser Ile His Asp Ser Met Leu Gln Thr Asp Asp Glu Ala Thr Pro Leu

225 230 235 240

aaa aag aag ctg gac gag ttt ggc agc ttt ttg gct aag gta att gcg 768

Lys Lys Lys Leu Asp Glu Phe Gly Ser Phe Leu Ala Lys Val Ile Ala

245 250 255

ggt att tgt gta ctt gtg tgg gtt gtc aac att ggt cac ttc agt gac 816

Gly Ile Cys Val Leu Val Trp Val Val Asn Ile Gly His Phe Ser Asp

260 265 270

cct tct cat ggt gga ttt ttt aaa ggc gca att cac tat ttt aag att 864

Pro Ser His Gly Gly Phe Phe Lys Gly Ala Ile His Tyr Phe Lys Ile

275 280 285

gca gtt gcc ctt gct gtt gca gct att cct gaa gga ctt cct gct gtc 912

Ala Val Ala Leu Ala Val Ala Ala Ile Pro Glu Gly Leu Pro Ala Val

290 295 300

gtg aca acg tgt tta gct ctt gga aca aag aaa atg gct cgt ttg aat 960

Val Thr Thr Cys Leu Ala Leu Gly Thr Lys Lys Met Ala Arg Leu Asn

305 310 315 320

gct att gta cgg tca tta cca tct gtc gag acg ctt ggg tgc act act 1008

Ala Ile Val Arg Ser Leu Pro Ser Val Glu Thr Leu Gly Cys Thr Thr

325 330 335

gta att tgc agt gac aag act gga aca ttg aca acc aat atg atg tcg 1056

Val Ile Cys Ser Asp Lys Thr Gly Thr Leu Thr Thr Asn Met Met Ser

340 345 350

gtg tct aag ata tgt gta gtc caa tct gca gag cat ggt cct atg att 1104

Val Ser Lys Ile Cys Val Val Gln Ser Ala Glu His Gly Pro Met Ile

355 360 365

aat gaa ttc act gtt agt ggg aca act tat gca cca gaa ggt acc gtc 1152

Asn Glu Phe Thr Val Ser Gly Thr Thr Tyr Ala Pro Glu Gly Thr Val

370 375 380

ttt gac agc aat ggg atg cag ctt gac tta cct gct cag tca cct tgc 1200

Phe Asp Ser Asn Gly Met Gln Leu Asp Leu Pro Ala Gln Ser Pro Cys

385 390 395 400

ctt cat cat tta gca atg tgt tca tca ctc tgc aat gac tcc atc ttg 1248

Leu His His Leu Ala Met Cys Ser Ser Leu Cys Asn Asp Ser Ile Leu

405 410 415

caa tac aat cca gat aag gat tct tat gaa aaa att gga gag tca act 1296

Gln Tyr Asn Pro Asp Lys Asp Ser Tyr Glu Lys Ile Gly Glu Ser Thr

420 425 430

gaa gtt gct ctt cga gtt ctt gca gaa aag gtt ggg ctc cct ggt ttt 1344

Glu Val Ala Leu Arg Val Leu Ala Glu Lys Val Gly Leu Pro Gly Phe

435 440 445

gat tca atg cct tct gct cta aac atg ttg agc aag cat gaa cgt gca 1392

Asp Ser Met Pro Ser Ala Leu Asn Met Leu Ser Lys His Glu Arg Ala

450 455 460

tca tat tgc aac cat tat tgg gaa aac caa ttc aaa aag gtt tat gtt 1440

Ser Tyr Cys Asn His Tyr Trp Glu Asn Gln Phe Lys Lys Val Tyr Val

465 470 475 480

ttg gag ttt act cgt gac cga aaa atg atg agc gtc cta tgt agc cat 1488

Leu Glu Phe Thr Arg Asp Arg Lys Met Met Ser Val Leu Cys Ser His

485 490 495

aag caa atg gat gtt atg ttc tca aag ggt gct cca gag agt ata ata 1536

Lys Gln Met Asp Val Met Phe Ser Lys Gly Ala Pro Glu Ser Ile Ile

500 505 510

gct agg tgt aat aaa att ctc tgc aac ggt gat ggt tct gtt gtt cct 1584

Ala Arg Cys Asn Lys Ile Leu Cys Asn Gly Asp Gly Ser Val Val Pro

515 520 525

cta act gct gct ggc cgt gca gag ctt gag tcg agg ttt tac agt ttt 1632

Leu Thr Ala Ala Gly Arg Ala Glu Leu Glu Ser Arg Phe Tyr Ser Phe

530 535 540

ggc gat gaa aca ttg aga tgc tta gca tta gca ttt aag acc gtg ccc 1680

Gly Asp Glu Thr Leu Arg Cys Leu Ala Leu Ala Phe Lys Thr Val Pro

545 550 555 560

cac ggt caa caa act att tcc tat gat aat gag aac gac ctg acg ttt 1728

His Gly Gln Gln Thr Ile Ser Tyr Asp Asn Glu Asn Asp Leu Thr Phe

565 570 575

att ggg ttg gtg gga atg ctt gat cca cca aga gaa gaa gtg aga gat 1776

Ile Gly Leu Val Gly Met Leu Asp Pro Pro Arg Glu Glu Val Arg Asp

580 585 590

gct atg ctt gcg tgt atg act gct ggg ata cgt gtt ata gtt gtt act 1824

Ala Met Leu Ala Cys Met Thr Ala Gly Ile Arg Val Ile Val Val Thr

595 600 605

ggg gat aac aag tcc aca gca gag tca cta tgt aga aaa ata ggg gct 1872

Gly Asp Asn Lys Ser Thr Ala Glu Ser Leu Cys Arg Lys Ile Gly Ala

610 615 620

ttt gac aat ctg gta gac ttt tct ggt atg tcc tac acc gct tct gaa 1920

Phe Asp Asn Leu Val Asp Phe Ser Gly Met Ser Tyr Thr Ala Ser Glu

625 630 635 640

ttt gaa cgg ctt cca gca gtg cag caa act cta gca ttg cga cgg atg 1968

Phe Glu Arg Leu Pro Ala Val Gln Gln Thr Leu Ala Leu Arg Arg Met

645 650 655

aca ctt ttt tcc agg gtt gaa cct tcc cac aaa agg atg ctt gtt gaa 2016

Thr Leu Phe Ser Arg Val Glu Pro Ser His Lys Arg Met Leu Val Glu

660 665 670

gcc cta cag aaa caa aac gaa gtg gtg gca atg act ggt gat ggc gtt 2064

Ala Leu Gln Lys Gln Asn Glu Val Val Ala Met Thr Gly Asp Gly Val

675 680 685

aat gat gcc cct gca ttg aag aaa gct gac att ggg att gcc atg ggt 2112

Asn Asp Ala Pro Ala Leu Lys Lys Ala Asp Ile Gly Ile Ala Met Gly

690 695 700

tct gga aca gct gta gca aag agt gct tca gat atg gtt ttg gct gat 2160

Ser Gly Thr Ala Val Ala Lys Ser Ala Ser Asp Met Val Leu Ala Asp

705 710 715 720

gat aat ttt gct tca ata gtt gcg gct gtt gca gaa gga agg gct ata 2208

Asp Asn Phe Ala Ser Ile Val Ala Ala Val Ala Glu Gly Arg Ala Ile

725 730 735

tat aat aac aca aag caa ttc att aga tac atg att tct tca aat ata 2256

Tyr Asn Asn Thr Lys Gln Phe Ile Arg Tyr Met Ile Ser Ser Asn Ile

740 745 750

ggg gaa gtg gtc tgt ata ttt gtt gca gct gta ctg gga atc cct gat 2304

Gly Glu Val Val Cys Ile Phe Val Ala Ala Val Leu Gly Ile Pro Asp

755 760 765

acc ttg gca cct gtt caa ctt ctg tgg gtc aat ttg gta aca gat gga 2352

Thr Leu Ala Pro Val Gln Leu Leu Trp Val Asn Leu Val Thr Asp Gly

770 775 780

ttg cct gcc act gcc att ggc ttt aat aaa caa gat tcc gat gtt atg 2400

Leu Pro Ala Thr Ala Ile Gly Phe Asn Lys Gln Asp Ser Asp Val Met

785 790 795 800

aag gca aaa ccc cga aag gtt ggt gaa gca gtg gtc act ggg tgg tta 2448

Lys Ala Lys Pro Arg Lys Val Gly Glu Ala Val Val Thr Gly Trp Leu

805 810 815

ttc ttc cgc tat ttg gtt atc gga gtt tat gtc ggc ctg gcc act gtt 2496

Phe Phe Arg Tyr Leu Val Ile Gly Val Tyr Val Gly Leu Ala Thr Val

820 825 830

gct ggc ttt ata tgg tgg ttt gtt tac tct gat ggt ggt cct aaa ctt 2544

Ala Gly Phe Ile Trp Trp Phe Val Tyr Ser Asp Gly Gly Pro Lys Leu

835 840 845

act tac agt gaa ctg atg aac ttt gaa act tgc gca ctt aga gag aca 2592

Thr Tyr Ser Glu Leu Met Asn Phe Glu Thr Cys Ala Leu Arg Glu Thr

850 855 860

act tat ccc tgc agc ata ttt gag gat cgg cac cca tct act gtg gct 2640

Thr Tyr Pro Cys Ser Ile Phe Glu Asp Arg His Pro Ser Thr Val Ala

865 870 875 880

atg aca gta ctt gtt gtt gtc gag atg ttt aat gct cta aat aac ctc 2688

Met Thr Val Leu Val Val Val Glu Met Phe Asn Ala Leu Asn Asn Leu

885 890 895

agc gaa aat caa tcc ctt ctg gtt ata acc cca agg agt aac tta tgg 2736

Ser Glu Asn Gln Ser Leu Leu Val Ile Thr Pro Arg Ser Asn Leu Trp

900 905 910

ctt gtt ggt tca att atc ctg acg atg ctt ctg cac gtg cta ata tta 2784

Leu Val Gly Ser Ile Ile Leu Thr Met Leu Leu His Val Leu Ile Leu

915 920 925

tat gtt cat cca ctg gca gtc tta ttt tct gtc acg cca tta tcc tgg 2832

Tyr Val His Pro Leu Ala Val Leu Phe Ser Val Thr Pro Leu Ser Trp

930 935 940

gcc gag tgg act gct gtt ctg tat ctt tcg ttt cca gtt atc atc atc 2880

Ala Glu Trp Thr Ala Val Leu Tyr Leu Ser Phe Pro Val Ile Ile Ile

945 950 955 960

gat gag ctt ctg aag ttc ctc tct aga aat aca ggc atg aga ttc agg 2928

Asp Glu Leu Leu Lys Phe Leu Ser Arg Asn Thr Gly Met Arg Phe Arg

965 970 975

ttc aga ttg agg aag gct gat tta ctc ccc aag gac cgg cgt gac aag 2976

Phe Arg Leu Arg Lys Ala Asp Leu Leu Pro Lys Asp Arg Arg Asp Lys

980 985 990

tag 2979

<210>22

<211>992

<212>PRT

＜213〉Arabidopis thaliana

<400>22

Met Glu Asp Ala Tyr Ala Arg Ser Val Ser Glu Val Leu Asp Phe Phe

1 5 10 15

Gly Val Asp Pro Thr Lys Gly Leu Ser Asp Ser Gln Val Val His His

20 25 30

Ser Arg Leu Tyr Gly Arg Asn Gly Thr Pro Phe Trp Lys Leu Val Leu

35 40 45

Lys Gln Phe Asp Asp Leu Leu Val Lys Ile Leu Ile Val Ala Ala Ile

50 55 60

Val Ser Phe Val Leu Ala Leu Ala Asn Gly Glu Thr Gly Leu Thr Ala

65 70 75 80

Phe Leu Glu Pro Phe Val Ile Leu Leu Ile Leu Ala Ala Asn Ala Ala

85 90 95

Val Gly Val Ile Thr Glu Thr Asn Ala Glu Lys Ala Leu Glu Glu Leu

100 105 110

Arg Ala Tyr Gln Ala Asn Ile Ala Thr Val Leu Arg Asn Gly Cys Phe

115 120 125

Ser Ile Leu Pro Ala Thr Glu Leu Val Pro Gly Asp Ile Val Glu Val

130 135 140

Thr Val Gly Cys Lys Ile Pro Ala Asp Leu Arg Met Ile Glu Met Ser

145 150 155 160

Ser Asn Thr Phe Arg Val Asp Gln Ala Ile Leu Thr Gly Glu Ser Cys

165 170 175

Ser Val Glu Lys Asp Val Asp Cys Thr Leu Thr Thr Asn Ala Val Tyr

180 185 190

Gln Asp Lys Lys Asn Ile Leu Phe Ser Gly Thr Asp Val Val Ala Gly

195 200 205

Arg Gly Arg Ala Val Val Ile Gly Val Gly Ser Asn Thr Ala Met Gly

210 215 220

Ser Ile His Asp Ser Met Leu Gln Thr Asp Asp Glu Ala Thr Pro Leu

225 230 235 240

Lys Lys Lys Leu Asp Glu Phe Gly Ser Phe Leu Ala Lys Val Ile Ala

245 250 255

Gly Ile Cys Val Leu Val Trp Val Val Asn Ile Gly His Phe Ser Asp

260 265 270

Pro Ser His Gly Gly Phe Phe Lys Gly Ala Ile His Tyr Phe Lys Ile

275 280 285

Ala Val Ala Leu Ala Val Ala Ala Ile Pro Glu Gly Leu Pro Ala Val

290 295 300

Val Thr Thr Cys Leu Ala Leu Gly Thr Lys Lys Met Ala Arg Leu Asn

305 310 315 320

Ala Ile Val Arg Ser Leu Pro Ser Val Glu Thr Leu Gly Cys Thr Thr

325 330 335

Val Ile Cys Ser Asp Lys Thr Gly Thr Leu Thr Thr Asn Met Met Ser

340 345 350

Val Ser Lys Ile Cys Val Val Gln Ser Ala Glu His Gly Pro Met Ile

355 360 365

Asn Glu Phe Thr Val Ser Gly Thr Thr Tyr Ala Pro Glu Gly Thr Val

370 375 380

Phe Asp Ser Asn Gly Met Gln Leu Asp Leu Pro Ala Gln Ser Pro Cys

385 390 395 400

Leu His His Leu Ala Met Cys Ser Ser Leu Cys Asn Asp Ser Ile Leu

405 410 415

Gln Tyr Asn Pro Asp Lys Asp Ser Tyr Glu Lys Ile Gly Glu Ser Thr

420 425 430

Glu Val Ala Leu Arg Val Leu Ala Glu Lys Val Gly Leu Pro Gly Phe

435 440 445

Asp Ser Met Pro Ser Ala Leu Asn Met Leu Ser Lys His Glu Arg Ala

450 455 460

Ser Tyr Cys Asn His Tyr Trp Glu Asn Gln Phe Lys Lys Val Tyr Val

465 470 475 480

Leu Glu Phe Thr Arg Asp Arg Lys Met Met Ser Val Leu Cys Ser His

485 490 495

Lys Gln Met Asp Val Met Phe Ser Lys Gly Ala Pro Glu Ser Ile Ile

500 505 510

Ala Arg Cys Asn Lys Ile Leu Cys Asn Gly Asp Gly Ser Val Val Pro

515 520 525

Leu Thr Ala Ala Gly Arg Ala Glu Leu Glu Ser Arg Phe Tyr Ser Phe

530 535 540

Gly Asp Glu Thr Leu Arg Cys Leu Ala Leu Ala Phe Lys Thr Val Pro

545 550 555 560

His Gly Gln Gln Thr Ile Ser Tyr Asp Asn Glu Asn Asp Leu Thr Phe

565 570 575

Ile Gly Leu Val Gly Met Leu Asp Pro Pro Arg Glu Glu Val Arg Asp

580 585 590

Ala Met Leu Ala Cys Met Thr Ala Gly Ile Arg Val Ile Val Val Thr

595 600 605

Gly Asp Asn Lys Ser Thr Ala Glu Ser Leu Cys Arg Lys Ile Gly Ala

610 615 620

Phe Asp Asn Leu Val Asp Phe Ser Gly Met Ser Tyr Thr Ala Ser Glu

625 630 635 640

Phe Glu Arg Leu Pro Ala Val Gln Gln Thr Leu Ala Leu Arg Arg Met

645 650 655

Thr Leu Phe Ser Arg Val Glu Pro Ser His Lys Arg Met Leu Val Glu

660 665 670

Ala Leu Gln Lys Gln Asn Glu Val Val Ala Met Thr Gly Asp Gly Val

675 680 685

Asn Asp Ala Pro Ala Leu Lys Lys Ala Asp Ile Gly Ile Ala Met Gly

690 695 700

Ser Gly Thr Ala Val Ala Lys Ser Ala Ser Asp Met Val Leu Ala Asp

705 710 715 720

Asp Asn Phe Ala Ser Ile Val Ala Ala Val Ala Glu Gly Arg Ala Ile

725 730 735

Tyr Asn Asn Thr Lys Gln Phe Ile Arg Tyr Met Ile Ser Ser Asn Ile

740 745 750

Gly Glu Val Val Cys Ile Phe Val Ala Ala Val Leu Gly Ile Pro Asp

755 760 765

Thr Leu Ala Pro Val Gln Leu Leu Trp Val Asn Leu Val Thr Asp Gly

770 775 780

Leu Pro Ala Thr Ala Ile Gly Phe Asn Lys Gln Asp Ser Asp Val Met

785 790 795 800

Lys Ala Lys Pro Arg Lys Val Gly Glu Ala Val Val Thr Gly Trp Leu

805 810 815

Phe Phe Arg Tyr Leu Val Ile Gly Val Tyr Val Gly Leu Ala Thr Val

820 825 830

Ala Gly Phe Ile Trp Trp Phe Val Tyr Ser Asp Gly Gly Pro Lys Leu

835 840 845

Thr Tyr Ser Glu Leu Met Asn Phe Glu Thr Cys Ala Leu Arg Glu Thr

850 855 860

Thr Tyr Pro Cys Ser Ile Phe Glu Asp Arg His Pro Ser Thr Val Ala

865 870 875 880

Met Thr Val Leu Val Val Val Glu Met Phe Asn Ala Leu Asn Asn Leu

885 890 895

Ser Glu Asn Gln Ser Leu Leu Val Ile Thr Pro Arg Ser Asn Leu Trp

900 905 910

Leu Val Gly Ser Ile Ile Leu Thr Met Leu Leu His Val Leu Ile Leu

915 920 925

Tyr Val His Pro Leu Ala Val Leu Phe Ser Val Thr Pro Leu Ser Trp

930 935 940

Ala Glu Trp Thr Ala Val Leu Tyr Leu Ser Phe Pro Val Ile Ile Ile

945 950 955 960

Asp Glu Leu Leu Lys Phe Leu Ser Arg Asn Thr Gly Met Arg Phe Arg

965 970 975

Phe Arg Leu Arg Lys Ala Asp Leu Leu Pro Lys Asp Arg Arg Asp Lys

980 985 990

<210>23

<211>1632

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(1632)

<400>23

atg gaa ggg gaa gaa gac act gtg gcg ggt tct agc ata cca aag aag 48

Met Glu Gly Glu Glu Asp Thr Val Ala Gly Ser Ser Ile Pro Lys Lys

1 5 10 15

aaa atg atg aaa cag ctg aca gga aaa cgc gac gac act ctg ctt cat 96

Lys Met Met Lys Gln Leu Thr Gly Lys Arg Asp Asp Thr Leu Leu His

20 25 30

tca gca gtg aga cac gga aac aaa gac aga gtt gtt gag att ctt acg 144

Ser Ala Val Arg His Gly Asn Lys Asp Arg Val Val Glu Ile Leu Thr

35 40 45

aaa acc aga gag tct gag ttg aat cag ctg ttg ggg aaa cag aac cag 192

Lys Thr Arg Glu Ser Glu Leu Asn Gln Leu Leu Gly Lys Gln Asn Gln

50 55 60

tca ggc gaa acc gca ctc tat gtt gca gca gag tat ggt gat gta gag 240

Ser Gly Glu Thr Ala Leu Tyr Val Ala Ala Glu Tyr Gly Asp Val Glu

65 70 75 80

att gtc aag gag atg atc aac tgc tat gat ctt gct ctc gtt gag atc 288

Ile Val Lys Glu Met Ile Asn Cys Tyr Asp Leu Ala Leu Val Glu Ile

85 90 95

aaa gca agg aac gga ttt gat gct ttc cac att gct gca aag caa gga 336

Lys Ala Arg Asn Gly Phe Asp Ala Phe His Ile Ala Ala Lys Gln Gly

100 105 110

gat ctc gat gtg ttg aag gtt tta gca gag gct cat tcg gag tta gcg 384

Asp Leu Asp Val Leu Lys Val Leu Ala Glu Ala His Ser Glu Leu Ala

115 120 125

atg acg gtg gat cta tca aac act acg gca ctg cac aca gcg gca aca 432

Met Thr Val Asp Leu Ser Asn Thr Thr Ala Leu His Thr Ala Ala Thr

130 135 140

caa gga cac act gaa gtg gta aac ttt ctt ttg gaa ctg gga agc agc 480

Gln Gly His Thr Glu Val Val Asn Phe Leu Leu Glu Leu Gly Ser Ser

145 150 155 160

ctt gct gga att gcc aag agc aat ggt aag acg gcc ctg cac tct gca 528

Leu Ala Gly Ile Ala Lys Ser Asn Gly Lys Thr Ala Leu His Ser Ala

165 170 175

tca agg aac ggg cat gtc aaa gtc att aag gct ctc ttg gca tcc gaa 576

Ser Arg Asn Gly His Val Lys Val Ile Lys Ala Leu Leu Ala Ser Glu

180 185 190

cct gcg atc gca ata agg atg gac aag aag ggc caa aca gcc ctt cac 624

Pro Ala Ile Ala Ile Arg Met Asp Lys Lys Gly Gln Thr Ala Leu His

195 200 205

atg gcg gtt aaa gga aca aat gtt gag gtc gtg gag gaa ctt atc aaa 672

Met Ala Val Lys Gly Thr Asn Val Glu Val Val Glu Glu Leu Ile Lys

210 215 220

gca gat agg tct tct atc aat ata gcc gac aca aag gga aac aca gcg 720

Ala Asp Arg Ser Ser Ile Asn Ile Ala Asp Thr Lys Gly Asn Thr Ala

225 230 235 240

ttg cac att gca gcc cga aaa ggc aga tct cag att gtc aag ttg cta 768

Leu His Ile Ala Ala Arg Lys Gly Arg Ser Gln Ile Val Lys Leu Leu

245 250 255

tta gcc aac aac atg aca gac aca aaa gct gtt aac cga tca ggc gaa 816

Leu Ala Asn Asn Met Thr Asp Thr Lys Ala Val Asn Arg Ser Gly Glu

260 265 270

acc gca ctt gac aca gca gag aaa att gga aat cca gaa gtg gct ctt 864

Thr Ala Leu Asp Thr Ala Glu Lys Ile Gly Asn Pro Glu Val Ala Leu

275 280 285

att tta cag aaa cat ggt gtt ccc agc gcc aag acc att aag cca tcc 912

Ile Leu Gln Lys His Gly Val Pro Ser Ala Lys Thr Ile Lys Pro Ser

290 295 300

ggg cct aac ccc gct cgg gaa ctg aaa caa acc gta agc gat atc aag 960

Gly Pro Asn Pro Ala Arg Glu Leu Lys Gln Thr Val Ser Asp Ile Lys

305 310 315 320

cat gag gtt cac aat cag ctt gag cac aca cgc ctg acc aga aaa cgt 1008

His Glu Val His Asn Gln Leu Glu His Thr Arg Leu Thr Arg Lys Arg

325 330 335

gtt caa gga atc gcc aaa cag ctt aac aaa atg cac act gaa ggt ctt 1056

Val Gln Gly Ile Ala Lys Gln Leu Asn Lys Met His Thr Glu Gly Leu

340 345 350

aac aat gca atc aac tcg act act gtt gta gct gtt ctt att gcc acg 1104

Asn Asn Ala Ile Asn Ser Thr Thr Val Val Ala Val Leu Ile Ala Thr

355 360 365

gtc gct ttt gca gca att ttc act gtc ccg ggg cag tat gta gaa gac 1152

Val Ala Phe Ala Ala Ile Phe Thr Val Pro Gly Gln Tyr Val Glu Asp

370 375 380

aca agt aaa att cca gat ggg cat tcc ctc ggg gag gcg aat att gca 1200

Thr Ser Lys Ile Pro Asp Gly His Ser Leu Gly Glu Ala Asn Ile Ala

385 390 395 400

tcg acg act ccg ttc ata att ttc ttc atc ttt gat tcg atc gca ctc 1248

Ser Thr Thr Pro Phe Ile Ile Phe Phe Ile Phe Asp Ser Ile Ala Leu

405 410 415

ttc atc tcc tta gcg gtc gtg gtg gtt cag aca tca gtg gtg gta ata 1296

Phe Ile Ser Leu Ala Val Val Val Val Gln Thr Ser Val Val Val Ile

420 425 430

gag agc aag gcc aag aaa cag atg atg gct gtg ata aac aaa ctc atg 1344

Glu Ser Lys Ala Lys Lys Gln Met Met Ala Val Ile Asn Lys Leu Met

435 440 445

tgg ctt gcc tgt gtt ctc atc tct gtt gcc ttt ttg gct ttg tcg ttt 1392

Trp Leu Ala Cys Val Leu Ile Ser Val Ala Phe Leu Ala Leu Ser Phe

450 455 460

gtt gtt gtt ggt gaa gaa gag aag tgg cta gcc att tgg gtg act gct 1440

Val Val Val Gly Glu Glu Glu Lys Trp Leu Ala Ile Trp Val Thr Ala

465 470 475 480

atc ggg gca act ata atg att acg acg tta ggg acg atg tgc tac tgg 1488

Ile Gly Ala Thr Ile Met Ile Thr Thr Leu Gly Thr Met Cys Tyr Trp

485 490 495

ata ata cag cac aag atc gaa gct gcc aat tta aga aac att aga aga 1536

Ile Ile Gln His Lys Ile Glu Ala Ala Asn Leu Arg Asn Ile Arg Arg

500 505 510

tcc tcc atc aac agt ata tct gga tcc tgg ggg att ccc cag ctt acg 1584

Ser Ser Ile Asn Ser Ile Ser Gly Ser Trp Gly Ile Pro Gln Leu Thr

515 520 525

gat tct gat att ctc cag aac gag tgt aag aaa atg tat gca atc tga 1632

Asp Ser Asp Ile Leu Gln Asn Glu Cys Lys Lys Met Tyr Ala Ile

530 535 540

<210>24

<211>543

<212>PRT

＜213〉Arabidopis thaliana

<400>24

Met Glu Gly Glu Glu Asp Thr Val Ala Gly Ser Ser Ile Pro Lys Lys

1 5 10 15

Lys Met Met Lys Gln Leu Thr Gly Lys Arg Asp Asp Thr Leu Leu His

20 25 30

Ser Ala Val Arg His Gly Asn Lys Asp Arg Val Val Glu Ile Leu Thr

35 40 45

Lys Thr Arg Glu Ser Glu Leu Asn Gln Leu Leu Gly Lys Gln Asn Gln

50 55 60

Ser Gly Glu Thr Ala Leu Tyr Val Ala Ala Glu Tyr Gly Asp Val Glu

65 70 75 80

Ile Val Lys Glu Met Ile Asn Cys Tyr Asp Leu Ala Leu Val Glu Ile

85 90 95

Lys Ala Arg Asn Gly Phe Asp Ala Phe His Ile Ala Ala Lys Gln Gly

100 105 110

Asp Leu Asp Val Leu Lys Val Leu Ala Glu Ala His Ser Glu Leu Ala

115 120 125

Met Thr Val Asp Leu Ser Asn Thr Thr Ala Leu His Thr Ala Ala Thr

130 135 140

Gln Gly His Thr Glu Val Val Asn Phe Leu Leu Glu Leu Gly Ser Ser

145 150 155 160

Leu Ala Gly Ile Ala Lys Ser Asn Gly Lys Thr Ala Leu His Ser Ala

165 170 175

Ser Arg Asn Gly His Val Lys Val Ile Lys Ala Leu Leu Ala Ser Glu

180 185 190

Pro Ala Ile Ala Ile Arg Met Asp Lys Lys Gly Gln Thr Ala Leu His

195 200 205

Met Ala Val Lys Gly Thr Asn Val Glu Val Val Glu Glu Leu Ile Lys

210 215 220

Ala Asp Arg Ser Ser Ile Asn Ile Ala Asp Thr Lys Gly Asn Thr Ala

225 230 235 240

Leu His Ile Ala Ala Arg Lys Gly Arg Ser Gln Ile Val Lys Leu Leu

245 250 255

Leu Ala Asn Asn Met Thr Asp Thr Lys Ala Val Asn Arg Ser Gly Glu

260 265 270

Thr Ala Leu Asp Thr Ala Glu Lys Ile Gly Asn Pro Glu Val Ala Leu

275 280 285

Ile Leu Gln Lys His Gly Val Pro Ser Ala Lys Thr Ile Lys Pro Ser

290 295 300

Gly Pro Asn Pro Ala Arg Glu Leu Lys Gln Thr Val Ser Asp Ile Lys

305 310 315 320

His Glu Val His Asn Gln Leu Glu His Thr Arg Leu Thr Arg Lys Arg

325 330 335

Val Gln Gly Ile Ala Lys Gln Leu Asn Lys Met His Thr Glu Gly Leu

340 345 350

Asn Asn Ala Ile Asn Ser Thr Thr Val Val Ala Val Leu Ile Ala Thr

355 360 365

Val Ala Phe Ala Ala Ile Phe Thr Val Pro Gly Gln Tyr Val Glu Asp

370 375 380

Thr Ser Lys Ile Pro Asp Gly His Ser Leu Gly Glu Ala Asn Ile Ala

385 390 395 400

Ser Thr Thr Pro Phe Ile Ile Phe Phe Ile Phe Asp Ser Ile Ala Leu

405 410 415

Phe Ile Ser Leu Ala Val Val Val Val Gln Thr Ser Val Val Val Ile

420 425 430

Glu Ser Lys Ala Lys Lys Gln Met Met Ala Val Ile Asn Lys Leu Met

435 440 445

Trp Leu Ala Cys Val Leu Ile Ser Val Ala Phe Leu Ala Leu Ser Phe

450 455 460

Val Val Val Gly Glu Glu Glu Lys Trp Leu Ala Ile Trp Val Thr Ala

465 470 475 480

Ile Gly Ala Thr Ile Met Ile Thr Thr Leu Gly Thr Met Cys Tyr Trp

485 490 495

Ile Ile Gln His Lys Ile Glu Ala Ala Asn Leu Arg Asn Ile Arg Arg

500 505 510

Ser Ser Ile Asn Ser Ile Ser Gly Ser Trp Gly Ile Pro Gln Leu Thr

515 520 525

Asp Ser Asp Ile Leu Gln Asn Glu Cys Lys Lys Met Tyr Ala Ile

530 535 540

<210>25

<211>801

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(801)

<400>25

atg gct tcc ttc gtg gaa tct ggt tgg cag tac ctt gtg aca cat ttt 48

Met Ala Ser Phe Val Glu Ser Gly Trp Gln Tyr Leu Val Thr His Phe

1 5 10 15

agc gac ttt caa ctg gca tgt att ggg agt ttt ctc ctc cat gaa agc 96

Ser Asp Phe Gln Leu Ala Cys Ile Gly Ser Phe Leu Leu His Glu Ser

20 25 30

gtg ttt ttc tta tct gga ctc cct ttc att ttt ctt gaa agg caa ggc 144

Val Phe Phe Leu Ser Gly Leu Pro Phe Ile Phe Leu Glu Arg Gln Gly

35 40 45

ttt ctc agc aag tac aaa att cag aca aaa aat aac aca cct gca gcc 192

Phe Leu Ser Lys Tyr Lys Ile Gln Thr Lys Asn Asn Thr Pro Ala Ala

50 55 60

caa gga aaa tgt att act cgc ctg ttg ctt tat cat ttc tcc gta aac 240

Gln Gly Lys Cys Ile Thr Arg Leu Leu Leu Tyr His Phe Ser Val Asn

65 70 75 80

ttg ccc ctg atg ttg gcc tcc tac cct gtc ttc cga gcc atg gga atg 288

Leu Pro Leu Met Leu Ala Ser Tyr Pro Val Phe Arg Ala Met Gly Met

85 90 95

cga agc agt ttt cct ctg ccg tcc tgg aaa gaa gtg tct gcc cag ata 336

Arg Ser Ser Phe Pro Leu Pro Ser Trp Lys Glu Val Ser Ala Gln Ile

100 105 110

tta ttc tac ttt atc att gag gat ttt gtc ttc tat tgg ggt cat cgg 384

Leu Phe Tyr Phe Ile Ile Glu Asp Phe Val Phe Tyr Trp Gly His Arg

115 120 125

atc ttg cat tca aaa tgg ctg tac aag aac gtg cat agt gtg cat cat 432

Ile Leu His Ser Lys Trp Leu Tyr Lys Asn Val His Ser Val His His

130 135 140

gaa tat gcc aca cca ttt ggt ttg aca tca gaa tat gct cac ccc gct 480

Glu Tyr Ala Thr Pro Phe Gly Leu Thr Ser Glu Tyr Ala His Pro Ala

145 150 155 160

gag att cta ttt ctg ggt ttt gct acc ata gtc ggt cca gct ctt act 528

Glu Ile Leu Phe Leu Gly Phe Ala Thr Ile Val Gly Pro Ala Leu Thr

165 170 175

ggc cct cac cta att act ctc tgg tta tgg atg gtg ttg aga gtg ctg 576

Gly Pro His Leu Ile Thr Leu Trp Leu Trp Met Val Leu Arg Val Leu

180 185 190

gag aca gtt gag gca cat tgt ggt tat cat ttc cca tgg agc ctc tca 624

Glu Thr Val Glu Ala His Cys Gly Tyr His Phe Pro Trp Ser Leu Ser

195 200 205

aat ttt ctt cct ctg tat gga ggt gct gac ttc cat gac tac cat cac 672

Asn Phe Leu Pro Leu Tyr Gly Gly Ala Asp Phe His Asp Tyr His His

210 215 220

cga ctg cta tac aca aag tcc gga aac tac tct tca act ttt gtg tat 720

Arg Leu Leu Tyr Thr Lys Ser Gly Asn Tyr Ser Ser Thr Phe Val Tyr

225 230 235 240

atg gac tgg atc ttt ggt act gac aag ggg tac aga aga ctg aag acc 768

Met Asp Trp Ile Phe Gly Thr Asp Lys Gly Tyr Arg Arg Leu Lys Thr

245 250 255

ctt aaa gaa aac ggt gac atg aaa caa acg tga 801

Leu Lys Glu Asn Gly Asp Met Lys Gln Thr

260 265

<210>26

<211>266

<212>PRT

＜213〉Arabidopis thaliana

<400>26

Met Ala Ser Phe Val Glu Ser Gly Trp Gln Tyr Leu Val Thr His Phe

1 5 10 15

Ser Asp Phe Gln Leu Ala Cys Ile Gly Ser Phe Leu Leu His Glu Ser

20 25 30

Val Phe Phe Leu Ser Gly Leu Pro Phe Ile Phe Leu Glu Arg Gln Gly

35 40 45

Phe Leu Ser Lys Tyr Lys Ile Gln Thr Lys Asn Asn Thr Pro Ala Ala

50 55 60

Gln Gly Lys Cys Ile Thr Arg Leu Leu Leu Tyr His Phe Ser Val Asn

65 70 75 80

Leu Pro Leu Met Leu Ala Ser Tyr Pro Val Phe Arg Ala Met Gly Met

85 90 95

Arg Ser Ser Phe Pro Leu Pro Ser Trp Lys Glu Val Ser Ala Gln Ile

100 105 110

Leu Phe Tyr Phe Ile Ile Glu Asp Phe Val Phe Tyr Trp Gly His Arg

115 120 125

Ile Leu His Ser Lys Trp Leu Tyr Lys Asn Val His Ser Val His His

130 135 140

Glu Tyr Ala Thr Pro Phe Gly Leu Thr Ser Glu Tyr Ala His Pro Ala

145 150 155 160

Glu Ile Leu Phe Leu Gly Phe Ala Thr Ile Val Gly Pro Ala Leu Thr

165 170 175

Gly Pro His Leu Ile Thr Leu Trp Leu Trp Met Val Leu Arg Val Leu

180 185 190

Glu Thr Val Glu Ala His Cys Gly Tyr His Phe Pro Trp Ser Leu Ser

195 200 205

Asn Phe Leu Pro Leu Tyr Gly Gly Ala Asp Phe His Asp Tyr His His

210 215 220

Arg Leu Leu Tyr Thr Lys Ser Gly Asn Tyr Ser Ser Thr Phe Val Tyr

225 230 235 240

Met Asp Trp Ile Phe Gly Thr Asp Lys Gly Tyr Arg Arg Leu Lys Thr

245 250 255

Leu Lys Glu Asn Gly Asp Met Lys Gln Thr

260 265

<210>27

<211>7608

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(7608)

<400>27

atg gat tcc gtc tct aga ggt gcc gtt gct tca aca acc ggc ggt gct 48

Met Asp Ser Val Ser Arg Gly Ala Val Ala Ser Thr Thr Gly Gly Ala

1 5 10 15

gtg gaa gag ccg gag tat cta gct agg tat ctt gtt gtt aaa cat tca 96

Val Glu Glu Pro Glu Tyr Leu Ala Arg Tyr Leu Val Val Lys His Ser

20 25 30

tgg aga ggt cgt tat aag agg atc ctt tgt att tcg agc ggc gga att 144

Trp Arg Gly Arg Tyr Lys Arg Ile Leu Cys Ile Ser Ser Gly Gly Ile

35 40 45

gtt acg ctt gat cct aat act ctt gct gtt act aat tct tat gat act 192

Val Thr Leu Asp Pro Asn Thr Leu Ala Val Thr Asn Ser Tyr Asp Thr

50 55 60

gga agt aat ttt gat ggt gct tca cct ctg gtt gga aga gat gag aac 240

Gly Ser Asn Phe Asp Gly Ala Ser Pro Leu Val Gly Arg Asp Glu Asn

65 70 75 80

acg gag agt gtt ggt ggt gag ttt act gtc aat gtt aga acg gat ggg 288

Thr Glu Ser Val Gly Gly Glu Phe Thr Val Asn Val Arg Thr Asp Gly

85 90 95

aaa ggg aaa ttt aag gct atg aag ttc tct tct agg tgc aga gcg agt 336

Lys Gly Lys Phe Lys Ala Met Lys Phe Ser Ser Arg Cys Arg Ala Ser

100 105 110

att ttg acc gag ttg tat cgg ctt aga tgg aat caa att aga cct gtg 384

Ile Leu Thr Glu Leu Tyr Arg Leu Arg Trp Asn Gln Ile Arg Pro Val

115 120 125

gct gag ttt cag gtg cta cat ctt agg aga cgg aac gca gaa tgg gtt 432

Ala Glu Phe Gln Val Leu His Leu Arg Arg Arg Asn Ala Glu Trp Val

130 135 140

cct tat aaa ttg aag atc acc ttt gtc ggt ctg gag ctt gtc gac tca 480

Pro Tyr Lys Leu Lys Ile Thr Phe Val Gly Leu Glu Leu Val Asp Ser

145 150 155 160

aaa tct ggt aat tca cgc tgg att ttg gat ttc aga gac atg ggt tcc 528

Lys Ser Gly Asn Ser Arg Trp Ile Leu Asp Phe Arg Asp Met Gly Ser

165 170 175

cca gca atc att ctt ctc tct gat gca tac cgg aca aaa tct gcg gac 576

Pro Ala Ile Ile Leu Leu Ser Asp Ala Tyr Arg Thr Lys Ser Ala Asp

180 185 190

tct gct ggg ttt gtt ctg tgt ccc atg tat ggg aga aag tca aaa gct 624

Ser Ala Gly Phe Val Leu Cys Pro Met Tyr Gly Arg Lys Ser Lys Ala

195 200 205

ttt aga gct gca ccc ggg aca aca aat tcc tcc att gtc gca agt ttg 672

Phe Arg Ala Ala Pro Gly Thr Thr Asn Ser Ser Ile Val Ala Ser Leu

210 215 220

gct aag act gca aag tcc atg gtt ggg gta ttc ttg tca gtc gat gat 720

Ala Lys Thr Ala Lys Ser Met Val Gly Val Phe Leu Ser Val Asp Asp

225 230 235 240

tca caa ttg ctg aca gta tca gag tat atg aca cga agg gct aaa gaa 768

Ser Gln Leu Leu Thr Val Ser Glu Tyr Met Thr Arg Arg Ala Lys Glu

245 250 255

gca gtt gga gct gaa gaa act cct aat ggg tgg tgg tct gtt act aga 816

Ala Val Gly Ala Glu Glu Thr Pro Asn Gly Trp Trp Ser Val Thr Arg

260 265 270

tta aga tct gct gct cat gga act ctg aac atg cct gga cta agc tta 864

Leu Arg Ser Ala Ala His Gly Thr Leu Asn Met Pro Gly Leu Ser Leu

275 280 285

gca att ggc ccc aaa gga gga ctt ggt gag cat ggg gat gct gta gcc 912

Ala Ile Gly Pro Lys Gly Gly Leu Gly Glu His Gly Asp Ala Val Ala

290 295 300

ctt cag ctt att ctt act aag gcc tcc ctt gtt gag aga cga ata gat 960

Leu Gln Leu Ile Leu Thr Lys Ala Ser Leu Val Glu Arg Arg Ile Asp

305 310 315 320

aac tat gaa gtt gtt atc gtt cgt cct cta tct tca gta agt tca ctt 1008

Asn Tyr Glu Val Val Ile Val Arg Pro Leu Ser Ser Val Ser Ser Leu

325 330 335

gtc cgg ttc gct gag gaa ccc caa atg ttt gct atc gaa ttc agt gat 1056

Val Arg Phe Ala Glu Glu Pro Gln Met Phe Ala Ile Glu Phe Ser Asp

340 345 350

gga tgt cca gtt ctt gga cac tgc ccg ata cca gta tta cca agg ctt 1104

Gly Cys Pro Val Leu Gly His Cys Pro Ile Pro Val Leu Pro Arg Leu

355 360 365

act atg cct ggt cat cgc att gat cca cct tgt gga agg gtt agt ttg 1152

Thr Met Pro Gly His Arg Ile Asp Pro Pro Cys Gly Arg Val Ser Leu

370 375 380

atc tct gga cca caa cat ctt gtt gct gat ttg gaa act tgc tcc cta 1200

Ile Ser Gly Pro Gln His Leu Val Ala Asp Leu Glu Thr Cys Ser Leu

385 390 395 400

cat ctg aaa cat tta gct gct gct gca aaa gat gca gtt gcc gaa ggt 1248

His Leu Lys His Leu Ala Ala Ala Ala Lys Asp Ala Val Ala Glu Gly

405 410 415

ggt tct gtt cct ggt tgt agg gct aga tta tgg cgc aga ata agg gag 1296

Gly Ser Val Pro Gly Cys Arg Ala Arg Leu Trp Arg Arg Ile Arg Glu

420 425 430

ttc aat gct tgt atc ccg tat aca ggt gtg ccc gct aat agt gaa gtc 1344

Phe Asn Ala Cys Ile Pro Tyr Thr Gly Val Pro Ala Asn Ser Glu Val

435 440 445

cct gag gtg act ttg atg gca tta att aca atg cta cca tca act cca 1392

Pro Glu Val Thr Leu Met Ala Leu Ile Thr Met Leu Pro Ser Thr Pro

450 455 460

aat ctc cct gta gac gcc cct cct ttg cca cct cct tca ccc aaa gca 1440

Asn Leu Pro Val Asp Ala Pro Pro Leu Pro Pro Pro Ser Pro Lys Ala

465 470 475 480

gca gca act gtc att ggc ttt gtt aca tgt ttg cgt agg tta ttg tca 1488

Ala Ala Thr Val Ile Gly Phe Val Thr Cys Leu Arg Arg Leu Leu Ser

485 490 495

tcc agg agt gca gca tcc cat ata atg tca ttc cct gct gct gtt aac 1536

Ser Arg Ser Ala Ala Ser His Ile Met Ser Phe Pro Ala Ala Val Asn

500 505 510

agg ata atg ggt tta ctt agg aac ggt tct gaa ggt gta gct gct gaa 1584

Arg Ile Met Gly Leu Leu Arg Asn Gly Ser Glu Gly Val Ala Ala Glu

515 520 525

gct gcg ggg ctt att gcg tcc ctc ata ggc ggt tgg tca gca gat ctg 1632

Ala Ala Gly Leu Ile Ala Ser Leu Ile Gly Gly Trp Ser Ala Asp Leu

530 535 540

agc act gca cca gat tcc aga gga gaa aaa cat gca act atc atg cat 1680

Ser Thr Ala Pro Asp Ser Arg Gly Glu Lys His Ala Thr Ile Met His

545 550 555 560

acc aag tct gtt ttg ttt gct caa cag ggt tat gtt act att ctg gtc 1728

Thr Lys Ser Val Leu Phe Ala Gln Gln Gly Tyr Val Thr Ile Leu Val

565 570 575

aat cga ttg aaa ccc atg tca gtc tca cct ctg ttt tcc atg gcg att 1776

Asn Arg Leu Lys Pro Met Ser Val Ser Pro Leu Phe Ser Met Ala Ile

580 585 590

gtt gaa gtc ttt gag gct atg gtt tgt gat cea cac gga gag act acc 1824

Val Glu Val Phe Glu Ala Met Val Cys Asp Pro His Gly Glu Thr Thr

595 600 605

caa tac act gtt ttt gta gaa ttg tta cga cag ata gct gcc cta cga 1872

Gln Tyr Thr Val Phe Val Glu Leu Leu Arg Gln Ile Ala Ala Leu Arg

610 615 620

cgt cgt tta ttt gca ctc ttt gca cat cct gca gag agt gtt agg gaa 1920

Arg Arg Leu Phe Ala Leu Phe Ala His Pro Ala Glu Ser Val Arg Glu

625 630 635 640

acc att gct gtt atc atg cgt aca ata gct gaa gaa gat gca att gct 1968

Thr Ile Ala Val Ile Met Arg Thr Ile Ala Glu Glu Asp Ala Ile Ala

645 650 655

gca gag tca atg cgt gat gct gct ttg cgc gat ggt gct ttg ttg aga 2016

Ala Glu Ser Met Arg Asp Ala Ala Leu Arg Asp Gly Ala Leu Leu Arg

660 665 670

cat tta ttg aat gca ttt tcc ctt cct gcc agt gag cgg cgc gag gta 2064

His Leu Leu Asn Ala Phe Ser Leu Pro Ala Ser Glu Arg Arg Glu Val

675 680 685

agt agg cag ctt gtg gca ctc tgg gca gat tct tac caa cca gct ttg 2112

Ser Arg Gln Leu Val Ala Leu Trp Ala Asp Ser Tyr Gln Pro Ala Leu

690 695 700

gat cta ctg tct cga gtt ctg cct cct ggg ctt gtt gca tat ttg cat 2160

Asp Leu Leu Ser Arg Val Leu Pro Pro Gly Leu Val Ala Tyr Leu His

705 710 715 720

aca cgt ccc gat gat gtt gtc gat gat aca gat caa gaa ggt tct tca 2208

Thr Arg Pro Asp Asp Val Val Asp Asp Thr Asp Gln Glu Gly Ser Ser

725 730 735

aca aat agg cgg cag aaa aga tta ctt cag cag aga aga ggt cgc ata 2256

Thr Asn Arg Arg Gln Lys Arg Leu Leu Gln Gln Arg Arg Gly Arg Ile

740 745 750

gct aag gga atg ggt gct caa gat att cct ctt ccc cct ggt aat aat 2304

Ala Lys Gly Met Gly Ala Gln Asp Ile Pro Leu Pro Pro Gly Asn Asn

755 760 765

gtt gag gct ggc gat gca gca aaa cat atg agt gca aat gct agt gta 2352

Val Glu Ala Gly Asp Ala Ala Lys His Met Ser Ala Asn Ala Ser Val

770 775 780

ccc gat aac ttt caa agg cgg gca gca gat tct tcc tct gaa gct tcc 2400

Pro Asp Asn Phe Gln Arg Arg Ala Ala Asp Ser Ser Ser Glu Ala Ser

785 790 795 800

aat cct cag gct tct gct ttt cca ggt gtt gac agt act att gca ggg 2448

Asn Pro Gln Ala Ser Ala Phe Pro Gly Val Asp Ser Thr Ile Ala Gly

805 810 815

gtt tca caa aat ggc tat cca gca ttt gct tca gtc acc aca aat gca 2496

Val Ser Gln Asn Gly Tyr Pro Ala Phe Ala Ser Val Thr Thr Asn Ala

820 825 830

aat ggg cat gag caa cct gag act aat gca tcc gat gtg gtt ggt tct 2544

Asn Gly His Glu Gln Pro Glu Thr Asn Ala Ser Asp Val Val Gly Ser

835 840 845

gac cca aac ttg tat ggc atc cag aat tca gtg ctt cca gca cct gct 2592

Asp Pro Asn Leu Tyr Gly Ile Gln Asn Ser Val Leu Pro Ala Pro Ala

850 855 860

caa gtt att gta gaa agt aca gct gta gga tcc gga aag cta ctt cta 2640

Gln Val Ile Val Glu Ser Thr Ala Val Gly Ser Gly Lys Leu Leu Leu

865 870 875 880

aat tgg cgt gag ttt tgg cga gcc ttt ggc ctt gat cat aat cgt gca 2688

Asn Trp Arg Glu Phe Trp Arg Ala Phe Gly Leu Asp His Asn Arg Ala

885 890 895

gat ctc atc tgg aat gag cgt aca agg caa gaa tta ata gaa gct ttg 2736

Asp Leu Ile Trp Asn Glu Arg Thr Arg Gln Glu Leu Ile Glu Ala Leu

900 905 910

aag gct gaa gtc cac aac cta gat gtc gag aaa gag cgc aca gaa gat 2784

Lys Ala Glu Val His Asn Leu Asp Val Glu Lys Glu Arg Thr Glu Asp

915 920 925

att tcc cct ggt gat gtc gag gcc aca act ggc cag gag att atc cca 2832

Ile Ser Pro Gly Asp Val Glu Ala Thr Thr Gly Gln Glu Ile Ile Pro

930 935 940

cgt ata tct tgg aac tat tct gaa ttc tct gtc agt tat cgt agc tta 2880

Arg Ile Ser Trp Asn Tyr Ser Glu Phe Ser Val Ser Tyr Arg Ser Leu

945 950 955 960

tca aaa gaa gtt tgt gtg ggc cag tat tac cta cgc tta ttg ctt gaa 2928

Ser Lys Glu Val Cys Val Gly Gln Tyr Tyr Leu Arg Leu Leu Leu Glu

965 970 975

agt ggc aac gct ggc aag gca caa gat ttc cct ctc cgt gat cca gtt 2976

Ser Gly Asn Ala Gly Lys Ala Gln Asp Phe Pro Leu Arg Asp Pro Val

980 985 990

gct ttt ttc agg gca ctc tat cat cgt ttc cag tgt gat gct gat atg 3024

Ala Phe Phe Arg Ala Leu Tyr His Arg Phe Gln Cys Asp Ala Asp Met

995 1000 1005

ggg ctt act att gat ggt gct gtt cca gat gaa ttg ggt tca tca 3069

Gly Leu Thr Ile Asp Gly Ala Val Pro Asp Glu Leu Gly Ser Ser

1010 1015 1020

ggc gac tgg tgt gat atg agt agg ctt gat ggt ttt ggt gga ggg 3114

Gly Asp Trp Cys Asp Met Ser Arg Leu Asp Gly Phe Gly Gly Gly

1025 1030 1035

gga gga gct tct gtt agg gag ctt tgt gca aga gca atg gcg att 3159

Gly Gly Ala Ser Val Arg Glu Leu Cys Ala Arg Ala Met Ala Ile

1040 1045 1050

gtc tat gag caa cac tac aac aca ata ggt cct ttt gaa ggc act 3204

Val Tyr Glu Gln His Tyr Asn Thr Ile Gly Pro Phe Glu Gly Thr

1055 1060 1065

gca cat att aca gca ctg att gat agg acg aat gat aga gct ttg 3249

Ala His Ile Thr Ala Leu Ile Asp Arg Thr Asn Asp Arg Ala Leu

1070 1075 1080

agg cat cgc cta cta ctt ctc cta aag gcc cta gtt aag gtc ttg 3294

Arg His Arg Leu Leu Leu Leu Leu Lys Ala Leu Val Lys Val Leu

1085 1090 1095

tta aac gtc gaa ggt tgt gtt gtg gtt ggt ggt tgt gtc cta gct 3339

Leu Asn Val Glu Gly Cys Val Val Val Gly Gly Cys Val Leu Ala

1100 1105 1110

gta gat ctg ctg act gtt gtt cat gaa aac tcg gag agg act cct 3384

Val Asp Leu Leu Thr Val Val His Glu Asn Ser Glu Arg Thr Pro

1115 1120 1125

att cca tta cag tcc aat tta att gct gct act gca ttt atg gaa 3429

Ile Pro Leu Gln Ser Asn Leu Ile Ala Ala Thr Ala Phe Met Glu

1130 1135 1140

cca cct aag gaa tgg atg tac ata gac aaa ggt ggt gca gaa gtg 3474

Pro Pro Lys Glu Trp Met Tyr Ile Asp Lys Gly Gly Ala Glu Val

1145 1150 1155

gga cct gta gag aag gac gtc atc aga agt tta tgg tcc aaa aag 3519

Gly Pro Val Glu Lys Asp Val Ile Arg Ser Leu Trp Ser Lys Lys

1160 1165 1170

gat att gac tgg acg aca aag tgt cgg gct tta gga atg tca gac 3564

Asp Ile Asp Trp Thr Thr Lys Cys Arg Ala Leu Gly Met Ser Asp

1175 1180 1185

tgg aag aaa ttg cgt gat atc cgt gaa ctt aga tgg gca gta gct 3609

Trp Lys Lys Leu Arg Asp Ile Arg Glu Leu Arg Trp Ala Val Ala

1190 1195 1200

gtt cga gtt cca gtc ctc aca cct agt cag gta ggg gat gct gca 3654

Val Arg Val Pro Val Leu Thr Pro Ser Gln Val Gly Asp Ala Ala

1205 1210 1215

ttg tcc ata tta cat agc atg gtt tcg gca cat tca gat ttg gat 3699

Leu Ser Ile Leu His Ser Met Val Ser Ala His Ser Asp Leu Asp

1220 1225 1230

gac gct gga gag att gta act cca aca cca aga gta aaa cgt atc 3744

Asp Ala Gly Glu Ile Val Thr Pro Thr Pro Arg Val Lys Arg Ile

1235 1240 1245

ttg tct agt aca cgt tgt ctt cct cac att gct cag gct ttg cta 3789

Leu Ser Ser Thr Arg Cys Leu Pro His Ile Ala Gln Ala Leu Leu

1250 1255 1260

tct ggc gaa cca gtt att gtg gag gct ggt gct gct ctc ttg aaa 3834

Ser Gly Glu Pro Val Ile Val Glu Ala Gly Ala Ala Leu Leu Lys

1265 1270 1275

gac gtt gtt acc aga aac tct aag gca atg atc cga ctg tac agt 3879

Asp Val Val Thr Arg Asn Ser Lys Ala Met Ile Arg Leu Tyr Ser

1280 1285 1290

aca ggg gcc ttt tac ttt gcc ctt gct tac cct gga tct aat ctt 3924

Thr Gly Ala Phe Tyr Phe Ala Leu Ala Tyr Pro Gly Ser Asn Leu

1295 1300 1305

tac tca atc gca caa ctc ttc tcg gtc acc cat gtc cat caa gct 3969

Tyr Ser Ile Ala Gln Leu Phe Ser Val Thr His Val His Gln Ala

1310 1315 1320

ttc cat ggt ggg gaa gaa gct act gtt tcc tcc tct ctg ccc ctg 4014

Phe His Gly Gly Glu Glu Ala Thr Val Ser Ser Ser Leu Pro Leu

132 51330 1335

gct aaa cga agc gta ttg ggt ggt ctt ctc cca gag tcc tta cta 4059

Ala Lys Arg Ser Val Leu Gly Gly Leu Leu Pro Glu Ser Leu Leu

1340 1345 1350

tat gta tta gag cgc agt gga cca gct gcg ttt gca gct ggc atg 4104

Tyr Val Leu Glu Arg Ser Gly Pro Ala Ala Phe Ala Ala Gly Met

1355 1360 1365

gtt tct gat tcc gat acg ccg gag att ata tgg aca cat aaa atg 4149

Val Ser Asp Ser Asp Thr Pro Glu Ile Ile Trp Thr His Lys Met

1370 1375 1380

cga gca gaa aat ctt ata tgt cag gtt ttg cag cat ctt ggt gat 4194

Arg Ala Glu Asn Leu Ile Cys Gln Val Leu Gln His Leu Gly Asp

1385 1390 1395

tat cct cag aaa ttg tca cag cac tgc cat tct ctc tat gat tat 4239

Tyr Pro Gln Lys Leu Ser Gln His Cys His Ser Leu Tyr Asp Tyr

1400 1405 1410

gct ccc atg cca cct gtt acg tat cca gaa ctt aga gat gag atg 4284

Ala Pro Met Pro Pro Val Thr Tyr Pro Glu Leu Arg Asp Glu Met

1415 1420 1425

tgg tgt cac cgt tat tat ctc aga aat tta tgt gat gag att caa 4329

Trp Cys His Arg Tyr Tyr Leu Arg Asn Leu Cys Asp Glu Ile Gln

1430 1435 1440

ttt cct aat tgg ccg att gtt gaa cat gtt gag ttc tta caa tca 4374

Phe Pro Asn Trp Pro Ile Val Glu His Val Glu Phe Leu Gln Ser

1445 1450 1455

tta ctt gtg atg tgg cgt gaa gag ttg act agg aaa ccc atg gat 4419

Leu Leu Val Met Trp Arg Glu Glu Leu Thr Arg Lys Pro Met Asp

1460 1465 1470

ctt tct gaa gga gaa gct tgc aaa att cta gaa ata tcc ctg aac 4464

Leu Ser Glu Gly Glu Ala Cys Lys Ile Leu Glu Ile Ser Leu Asn

1475 1480 1485

aat gta tca agt gat gac cta aac cgg act gct tca gtt gag ttg 4509

Asn Val Ser Ser Asp Asp Leu Asn Arg Thr Ala Ser Val Glu Leu

1490 1495 1500

aat gag gaa ata tct aat ata tcc aaa caa att caa aac ctt gat 4554

Asn Glu Glu Ile Ser Asn Ile Ser Lys Gln Ile Gln Asn Leu Asp

1505 1510 1515

gaa gag aaa cta aag cgc cag tat agg aag ctt gca atg agg tac 4599

Glu Glu Lys Leu Lys Arg Gln Tyr Arg Lys Leu Ala Met Arg Tyr

1520 1525 1530

cat cct gac aag aat cca gaa gga aga gaa aag ttc ctg gct gtt 4644

His Pro Asp Lys Asn Pro Glu Gly Arg Glu Lys Phe Leu Ala Val

1535 1540 1545

caa aaa gct tat gaa tgc cta cag gca aca atg caa gga ttg caa 4689

Gln Lys Ala Tyr Glu Cys Leu Gln Ala Thr Met Gln Gly Leu Gln

1550 1555 1560

ggt cct eag ccg tgg agg ttg ctg ctt tta ctg aaa gcg cag tgc 4734

Gly Pro Gln Pro Trp Arg Leu Leu Leu Leu Leu Lys Ala Gln Cys

1565 1570 1575

atc tta tat cgc cgt tat gga cat gtg tta cga ccg ttc aaa tat 4779

Ile Leu Tyr Arg Arg Tyr Gly His Val Leu Arg Pro Phe Lys Tyr

1580 1585 1590

gct ggc tat ccg atg tta ctt gat gca gtt aca gtg gac aag gat 4824

Ala Gly Tyr Pro Met Leu Leu Asp Ala Val Thr Val Asp Lys Asp

1595 1600 1605

gac aac aac ttt cta tct aat gat aga tcc cct ctt ctt gtt gca 4869

Asp Asn Asn Phe Leu Ser Asn Asp Arg Ser Pro Leu Leu Val Ala

1610 1615 1620

gca tct gag ctt gtt tcg tta acc tgt gct gcc tcg tca ttg aat 4914

Ala Ser Glu Leu Val Ser Leu Thr Cys Ala Ala Ser Ser Leu Asn

1625 1630 1635

ggt gaa gaa tta gtg aga gat ggt ggt gtg cag ctt cta tca act 4959

Gly Glu Glu Leu Val Arg Asp Gly Gly Val Gln Leu Leu Ser Thr

1640 1645 1650

ctt ctt tcc cgc tgc atg tgt gtg gtt cag cca aca act tca caa 5004

Leu Leu Ser Arg Cys Met Cys Val Val Gln Pro Thr Thr Ser Gln

1655 1660 1665

cac gaa cca gct gcg atc att gtc aca aat gta atg cgt aca ctt 5049

His Glu Pro Ala Ala Ile Ile Val Thr Asn Val Met Arg Thr Leu

1670 1675 1680

tcg gta ata agt cag ttt gag agt gcg agg gct gga ttt cta gag 5094

Ser Val Ile Ser Gln Phe Glu Ser Ala Arg Ala Gly Phe Leu Glu

1685 1690 1695

tta ccc agt ctg att gaa gac att gtg cac tgt acg gaa tta gaa 5139

Leu Pro Ser Leu Ile Glu Asp Ile Val His Cys Thr Glu Leu Glu

1700 1705 1710

cgt gtg cct gca gcc gtt gat gct gct ctc cag tcc att gcc aag 5184

Arg Val Pro Ala Ala Val Asp Ala Ala Leu Gln Ser Ile Ala Lys

1715 1720 1725

gtt tct gtc ttc ccc gaa ctt cag cat ggt ctg cta aag gct ggt 5229

Val Ser Val Phe Pro Glu Leu Gln His Gly Leu Leu Lys Ala Gly

1730 1735 1740

gcc tta tgg tat att ctc cca tta tta cta cag tat gac tca act 5274

Ala Leu Trp Tyr Ile Leu Pro Leu Leu Leu Gln Tyr Asp Ser Thr

1745 1750 1755

gct gag gaa tct aat tct gtc gag tct cat ggg gtt gga gtt agc 5319

Ala Glu Glu Ser Asn Ser Val Glu Ser His Gly Val Gly Val Ser

1760 1765 1770

att caa att gcc aag aat gag cat gcc tta caa gca tca caa gcc 5364

Ile Gln Ile Ala Lys Asn Glu His Ala Leu Gln Ala Ser Gln Ala

1775 1780 1785

cta tca agg ctt act ggg ctg tgt gca gat gag agt ttg aca cct 5409

Leu Ser Arg Leu Thr Gly Leu Cys Ala Asp Glu Ser Leu Thr Pro

1790 1795 1800

tac aat gct act gcg gct gat gtt ctc aaa gca tta ctg acg cca 5454

Tyr Asn Ala Thr Ala Ala Asp Val Leu Lys Ala Leu Leu Thr Pro

1805 1810 1815

aaa ctt gct agt ttg ttg aaa gat gaa gtt gcc aag gat ttg tta 5499

Lys Leu Ala Ser Leu Leu Lys Asp Glu Val Ala Lys Asp Leu Leu

1820 1825 1830

tcc aaa ctg aac aca aat ttg gag aca cca gag att atc tgg aac 5544

Ser Lys Leu Asn Thr Asn Leu Glu Thr Pro Glu Ile Ile Trp Asn

1835 1840 1845

tct gca act cga tca gag ctt tta aat ttt gtg gat gaa caa cgc 5589

Ser Ala Thr Arg Ser Glu Leu Leu Asn Phe Val Asp Glu Gln Arg

1850 1855 1860

gcc tgc cag tgc cct gat ggt tca tat gat ctg aaa aat gct caa 5634

Ala Cys Gln Cys Pro Asp Gly Ser Tyr Asp Leu Lys Asn Ala Gln

1865 1870 1875

tct ttt tcg tat gac gca ctg tca aaa gag gtc ttt gtt ggc aat 5679

Ser Phe Ser Tyr Asp Ala Leu Ser Lys Glu Val Phe Val Gly Asn

1880 1885 1890

gtt tac ttg aag gtc tat aat gat caa ccc gac tca gag atc agt 5724

Val Tyr Leu Lys Val Tyr Asn Asp Gln Pro Asp Ser Glu Ile Ser

1895 1900 1905

gaa cca gaa tca ttc tgc aat gcc cta atc gac ttt ata tca tca 5769

Glu Pro Glu Ser Phe Cys Asn Ala Leu Ile Asp Phe Ile Ser Ser

1910 1915 1920

tta gtg cat act gag ttg ccc tct gtt tcc gag gac caa aat ttg 5814

Leu Val His Thr Glu Leu Pro Ser Val Ser Glu Asp Gln Asn Leu

1925 1930 1935

atc gaa gac aga aac tca tct aat gat act cca gag ctt caa agt 5859

Ile Glu Asp Arg Asn Ser Ser Asn Asp Thr Pro Glu Leu Gln Ser

1940 1945 1950

agc gtc gca gaa ccg tcg ttg att gaa gaa cat tcc gat cat cag 5904

Ser Val Ala Glu Pro Ser Leu Ile Glu Glu His Ser Asp His Gln

1955 1960 1965

cca tca tct gag ggg atg aag aac gaa gaa tgt ttt ctg att gat 5949

Pro Ser Ser Glu Gly Met Lys Asn Glu Glu Cys Phe Leu Ile Asp

1970 1975 1980

cac ctc caa tta gga ttg act gct ctt cag aac ttg ctt aca aag 5994

His Leu Gln Leu Gly Leu Thr Ala Leu Gln Asn Leu Leu Thr Lys

1985 1990 1995

tat cca gat ctg gct tca gtg ttt tcg tct aag gag aga ttg tta 6039

Tyr Pro Asp Leu Ala Ser Val Phe Ser Ser Lys Glu Arg Leu Leu

2000 2005 2010

cct ctc ttt gaa tgt ttt tct gtg gcc att gca tca aaa aca gat 6084

Pro Leu Phe Glu Cys Phe Ser Val Ala Ile Ala Ser Lys Thr Asp

2015 2020 2025

att cca aaa ctc tgc ctc aat gtc ctc tct cgg tta aca gct tat 6129

Ile Pro Lys Leu Cys Leu Asn Val Leu Ser Arg Leu Thr Ala Tyr

2030 2035 2040

gct cct tgc ttg gag acg atg gta tct gat gga tct agt ctt ctt 6174

Ala Pro Cys Leu Glu Thr Met Val Ser Asp Gly Ser Ser Leu Leu

2045 2050 2055

ctc ctc tta caa atg ctt cat tct gca cct tct ttt cgc gag ggt 6219

Leu Leu Leu Gln Met Leu His Ser Ala Pro Ser Phe Arg Glu Gly

2060 2065 2070

gct ctc cat gtt ctt tat gct ttg gca agc aca cca gaa ctt gct 6264

Ala Leu His Val Leu Tyr Ala Leu Ala Ser Thr Pro Glu Leu Ala

2075 2080 2085

tgg gct gct gca aaa cat gaa gaa att ccc ttg cag caa aga gct 6309

Trp Ala Ala Ala Lys His Glu Glu Ile Pro Leu Gln Gln Arg Ala

2090 2095 2100

gca gcg gct tct ttg ttg ggg aag ctc gtc gca caa cca atg cat 6354

Ala Ala Ala Ser Leu Leu Gly Lys Leu Val Ala Gln Pro Met His

2105 2110 2115

ggg cct aga gtt gct atc aca ctt gtg aga ttc ctt cct gac ggt 6399

Gly Pro Arg Val Ala Ile Thr Leu Val Arg Phe Leu Pro Asp Gly

2120 2125 2130

ctt gta tct ata att cgt gat gga cct ggg gag gct gtt gtc cat 6444

Leu Val Ser Ile Ile Arg Asp Gly Pro Gly Glu Ala Val Val His

2135 2140 2145

gca ctt gag cgg acc act gag act cca gaa ctt gtg tgg aca cca 6489

Ala Leu Glu Arg Thr Thr Glu Thr Pro Glu Leu Val Trp Thr Pro

2150 2155 2160

gca atg gca gca tct tta tcc gca cag att gca acc atg gca tca 6534

Ala Met Ala Ala Ser Leu Ser Ala Gln Ile Ala Thr Met Ala Ser

2165 2170 2175

gat att tat cgt gaa caa cag aag ggt tct gtt att gaa tgg gat 6579

Asp Ile Tyr Arg Glu Gln Gln Lys Gly Ser Val Ile Glu Trp Asp

2180 2185 2190

gta cca gag cag tca gct ggt caa caa gaa atg aga gac gag cca 6624

Val Pro Glu Gln Ser Ala Gly Gln Gln Glu Met Arg Asp Glu Pro

2195 2200 2205

cag gtt ggt gga atc tat gtc agg cgt ttc tta aaa gat cca aaa 6669

Gln Val Gly Gly Ile Tyr Val Arg Arg Phe Leu Lys Asp Pro Lys

2210 2215 2220

ttt cct ctg aga aat cca aaa cga ttc ttg gaa gga ctg ctg gat 6714

Phe Pro Leu Arg Asn Pro Lys Arg Phe Leu Glu Gly Leu Leu Asp

2225 2230 2235

cag tat ttg tca gca atg gcc gca aca cat tac gaa caa cat cct 6759

Gln Tyr Leu Ser Ala Met Ala Ala Thr His Tyr Glu Gln His Pro

2240 2245 2250

gtt gac cct gag ctc cct ctc ctt ctc tct gct gca ttg gtt tct 6804

Val Asp Pro Glu Leu Pro Leu Leu Leu Ser Ala Ala Leu Val Ser

2255 2260 2265

ttg ttg cgt gtg cat cct gca ctt gca gat cac att gga cat ctt 6849

Leu Leu Arg Val His Pro Ala Leu Ala Asp His Ile Gly His Leu

2270 2275 2280

ggg tat gtc cca aaa ctt gtc gct gct gtg gca tat gag ggg agg 6894

Gly Tyr Val Pro Lys Leu Val Ala Ala Val Ala Tyr Glu Gly Arg

2285 2290 2295

cgg gaa aca atg tct tct ggc gaa gtg aag gct gaa gaa att ggc 6939

Arg Glu Thr Met Ser Ser Gly Glu Val Lys Ala Glu Glu Ile Gly

2300 2305 2310

tct gat gga gtg aat gag tct act gat ccc tca agt cta cct ggg 6984

Ser Asp Gly Val Asn Glu Ser Thr Asp Pro Ser Ser Leu Pro Gly

2315 2320 2325

caa acc cct caa gaa cgt gtg cgc ctt agt tgt tta cgt gtg ctt 7029

Gln Thr Pro Gln Glu Arg Val Arg Leu Ser Cys Leu Arg Val Leu

2330 2335 2340

cat caa ctt gca gct agt acc aca tgt gct gaa gca atg gct gca 7074

His Gln Leu Ala Ala Ser Thr Thr Cys Ala Glu Ala Met Ala Ala

2345 2350 2355

act agt gct gga aat gca cag gtg gtt cca ctt ctc atg aaa gca 7119

Thr Ser Ala Gly Asn Ala Gln Val Val Pro Leu Leu Met Lys Ala

2360 2365 2370

ata gga tgg ctt ggt gga agc att tta gca ctc gag aca ctt aag 7164

Ile Gly Trp Leu Gly Gly Ser Ile Leu Ala Leu Glu Thr Leu Lys

2375 2380 2385

cgt gtt gtt gtt gct gga aat cgg gcc aga gat gcg ctt gtt gcg 7209

Arg Val Val Val Ala Gly Asn Arg Ala Arg Asp Ala Leu Val Ala

2390 2395 2400

cag ggt cta aag gtt ggt ctc att gag gtt ctt ctt ggg ctg ctt 7254

Gln Gly Leu Lys Val Gly Leu Ile Glu Val Leu Leu Gly Leu Leu

2405 2410 2415

gac tgg agg acg ggg ggt agg tat ggg ctc agt tct cac atg aaa 7299

Asp Trp Arg Thr Gly Gly Arg Tyr Gly Leu Ser Ser His Met Lys

2420 2425 2430

tgg aat gaa tcg gaa gca tca atc ggg cgg gta ctt gca gtt gag 7344

Trp Asn Glu Ser Glu Ala Ser Ile Gly Arg Val Leu Ala Val Glu

2435 2440 2445

gtt agt gtt gaa ttt gtt agc gag atg ttt gtt atg tgt gtt aca 7389

Val Ser Val Glu Phe Val Ser Glu Met Phe Val Met Cys Val Thr

2450 2455 2460

cat gta ttg cat ggt ttt gca aca gaa gga gca cat tgc tca aaa 7434

His Val Leu His Gly Phe Ala Thr Glu Gly Ala His Cys Ser Lys

2465 2470 2475

gtg cgt gag ata ctt gac gcg tca gaa gtg tgg agt gca tat aaa 7479

Val Arg Glu Ile Leu Asp Ala Ser Glu Val Trp Ser Ala Tyr Lys

2480 2485 2490

gac caa aag cat gac ttg ttc ctg cca tca aac aca caa tca gcg 7524

Asp Gln Lys His Asp Leu Phe Leu Pro Ser Asn Thr Gln Ser Ala

2495 2500 2505

gca ggg gtg gct ggc ttt att gag aac tca tcc aac agt ctc act 7569

Ala Gly Val Ala Gly Phe Ile Glu Asn Ser Ser Asn Ser Leu Thr

2510 2515 2520

tac gct ctt acc gct cct cct ccg cct tcg cat cct tga 7608

Tyr Ala Leu Thr Ala Pro Pro Pro Pro Ser His Pro

2525 2530 2535

<210>28

<211>2535

<212>PRT

＜213〉Arabidopis thaliana

<400>28

Met Asp Ser Val Ser Arg Gly Ala Val Ala Ser Thr Thr Gly Gly Ala

1 5 10 15

Val Glu Glu Pro Glu Tyr Leu Ala Arg Tyr Leu Val Val Lys His Ser

20 25 30

Trp Arg Gly Arg Tyr Lys Arg Ile Leu Cys Ile Ser Ser Gly Gly Ile

35 40 45

Val Thr Leu Asp Pro Asn Thr Leu Ala Val Thr Asn Ser Tyr Asp Thr

50 55 60

Gly Ser Asn Phe Asp Gly Ala Ser Pro Leu Val Gly Arg Asp Glu Asn

65 70 75 80

Thr Glu Ser Val Gly Gly Glu Phe Thr Val Asn Val Arg Thr Asp Gly

85 90 95

Lys Gly Lys Phe Lys Ala Met Lys Phe Ser Ser Arg Cys Arg Ala Ser

100 105 110

Ile Leu Thr Glu Leu Tyr Arg Leu Arg Trp Asn Gln Ile Arg Pro Val

115 120 125

Ala Glu Phe Gln Val Leu His Leu Arg Arg Arg Asn Ala Glu Trp Val

130 135 140

Pro Tyr Lys Leu Lys Ile Thr Phe Val Gly Leu Glu Leu Val Asp Ser

145 150 155 160

Lys Ser Gly Asn Ser Arg Trp Ile Leu Asp Phe Arg Asp Met Gly Ser

165 170 175

Pro Ala Ile Ile Leu Leu Ser Asp Ala Tyr Arg Thr Lys Ser Ala Asp

180 185 190

Ser Ala Gly Phe Val Leu Cys Pro Met Tyr Gly Arg Lys Ser Lys Ala

195 200 205

Phe Arg Ala Ala Pro Gly Thr Thr Asn Ser Ser Ile Val Ala Ser Leu

210 215 220

Ala Lys Thr Ala Lys Ser Met Val Gly Val Phe Leu Ser Val Asp Asp

225 230 235 240

Ser Gln Leu Leu Thr Val Ser Glu Tyr Met Thr Arg Arg Ala Lys Glu

245 250 255

Ala Val Gly Ala Glu Glu Thr Pro Asn Gly Trp Trp Ser Val Thr Arg

260 265 270

Leu Arg Ser Ala Ala His Gly Thr Leu Asn Met Pro Gly Leu Ser Leu

275 280 285

Ala Ile Gly Pro Lys Gly Gly Leu Gly Glu His Gly Asp Ala Val Ala

290 295 300

Leu Gln Leu Ile Leu Thr Lys Ala Ser Leu Val Glu Arg Arg Ile Asp

305 310 315 320

Asn Tyr Glu Val Val Ile Val Arg Pro Leu Ser Ser Val Ser Ser Leu

325 330 335

Val Arg Phe Ala Glu Glu Pro Gln Met Phe Ala Ile Glu Phe Ser Asp

340 345 350

Gly Cys Pro Val Leu Gly His Cys Pro Ile Pro Val Leu Pro Arg Leu

355 360 365

Thr Met Pro Gly His Arg Ile Asp Pro Pro Cys Gly Arg Val Ser Leu

370 375 380

Ile Ser Gly Pro Gln His Leu Val Ala Asp Leu Glu Thr Cys Ser Leu

385 390 395 400

His Leu Lys His Leu Ala Ala Ala Ala Lys Asp Ala Val Ala Glu Gly

405 410 415

Gly Ser Val Pro Gly Cys Arg Ala Arg Leu Trp Arg Arg Ile Arg Glu

420 425 430

Phe Asn Ala Cys Ile Pro Tyr Thr Gly Val Pro Ala Asn Ser Glu Val

435 440 445

Pro Glu Val Thr Leu Met Ala Leu Ile Thr Met Leu Pro Ser Thr Pro

450 455 460

Asn Leu Pro Val Asp Ala Pro Pro Leu Pro Pro Pro Ser Pro Lys Ala

465 470 475 480

Ala Ala Thr Val Ile Gly Phe Val Thr Cys Leu Arg Arg Leu Leu Ser

485 490 495

Ser Arg Ser Ala Ala Ser His Ile Met Ser Phe Pro Ala Ala Val Asn

500 505 510

Arg Ile Met Gly Leu Leu Arg Asn Gly Ser Glu Gly Val Ala Ala Glu

515 520 525

Ala Ala Gly Leu Ile Ala Ser Leu Ile Gly Gly Trp Ser Ala Asp Leu

530 535 540

Ser Thr Ala Pro Asp Ser Arg Gly Glu Lys His Ala Thr Ile Met His

545 550 555 560

Thr Lys Ser Val Leu Phe Ala Gln Gln Gly Tyr Val Thr Ile Leu Val

565 570 575

Asn Arg Leu Lys Pro Met Ser Val Ser Pro Leu Phe Ser Met Ala Ile

580 585 590

Val Glu Val Phe Glu Ala Met Val Cys Asp Pro His Gly Glu Thr Thr

595 600 605

Gln Tyr Thr Val Phe Val Glu Leu Leu Arg Gln Ile Ala Ala Leu Arg

610 615 620

Arg Arg Leu Phe Ala Leu Phe Ala His Pro Ala Glu Ser Val Arg Glu

625 630 635 640

Thr Ile Ala Val Ile Met Arg Thr Ile Ala Glu Glu Asp Ala Ile Ala

645 650 655

Ala Glu Ser Met Arg Asp Ala Ala Leu Arg Asp Gly Ala Leu Leu Arg

660 665 670

His Leu Leu Asn Ala Phe Ser Leu Pro Ala Ser Glu Arg Arg Glu Val

675 680 685

Ser Arg Gln Leu Val Ala Leu Trp Ala Asp Ser Tyr Gln Pro Ala Leu

690 695 700

Asp Leu Leu Ser Arg Val Leu Pro Pro Gly Leu Val Ala Tyr Leu His

705 710 715 720

Thr Arg Pro Asp Asp Val Val Asp Asp Thr Asp Gln Glu Gly Ser Ser

725 730 735

Thr Asn Arg Arg Gln Lys Arg Leu Leu Gln Gln Arg Arg Gly Arg Ile

740 745 750

Ala Lys Gly Met Gly Ala Gln Asp Ile Pro Leu Pro Pro Gly Asn Asn

755 760 765

Val Glu Ala Gly Asp Ala Ala Lys His Met Ser Ala Asn Ala Ser Val

770 775 780

Pro Asp Asn Phe Gln Arg Arg Ala Ala Asp Ser Ser Ser Glu Ala Ser

785 790 795 800

Asn Pro Gln Ala Ser Ala Phe Pro Gly Val Asp Ser Thr Ile Ala Gly

805 810 815

Val Ser Gln Asn Gly Tyr Pro Ala Phe Ala Ser Val Thr Thr Asn Ala

820 825 830

Asn Gly His Glu Gln Pro Glu Thr Asn Ala Ser Asp Val Val Gly Ser

835 840 845

Asp Pro Asn Leu Tyr Gly Ile Gln Asn Ser Val Leu Pro Ala Pro Ala

850 855 860

Gln Val Ile Val Glu Ser Thr Ala Val Gly Ser Gly Lys Leu Leu Leu

865 870 875 880

Asn Trp Arg Glu Phe Trp Arg Ala Phe Gly Leu Asp His Asn Arg Ala

885 890 895

Asp Leu Ile Trp Asn Glu Arg Thr Arg Gln Glu Leu Ile Glu Ala Leu

900 905 910

Lys Ala Glu Val His Asn Leu Asp Val Glu Lys Glu Arg Thr Glu Asp

915 920 925

Ile Ser Pro Gly Asp Val Glu Ala Thr Thr Gly Gln Glu Ile Ile Pro

930 935 940

Arg Ile Ser Trp Asn Tyr Ser Glu Phe Ser Val Ser Tyr Arg Ser Leu

945 950 955 960

Ser Lys Glu Val Cys Val Gly Gln Tyr Tyr Leu Arg Leu Leu Leu Glu

965 970 975

Ser Gly Asn Ala Gly Lys Ala Gln Asp Phe Pro Leu Arg Asp Pro Val

980 985 990

Ala Phe Phe Arg Ala Leu Tyr His Arg Phe Gln Cys Asp Ala Asp Met

995 1000 1005

Gly Leu Thr Ile Asp Gly Ala Val Pro Asp Glu Leu Gly Ser Ser

1010 1015 1020

Gly Asp Trp Cys Asp Met Ser Arg Leu Asp Gly Phe Gly Gly Gly

1025 1030 1035

Gly Gly Ala Ser Val Arg Glu Leu Cys Ala Arg Ala Met Ala Ile

1040 1045 1050

Val Tyr Glu Gln His Tyr Asn Thr Ile Gly Pro Phe Glu Gly Thr

1055 1060 1065

Ala His Ile Thr Ala Leu Ile Asp Arg Thr Asn Asp Arg Ala Leu

1070 1075 1080

Arg His Arg Leu Leu Leu Leu Leu Lys Ala Leu Val Lys Val Leu

1085 1090 1095

Leu Asn Val Glu Gly Cys Val Val Val Gly Gly Cys Val Leu Ala

1100 1105 1110

Val Asp Leu Leu Thr Val Val His Glu Asn Ser Glu Arg Thr Pro

1115 1120 1125

Ile Pro Leu Gln Ser Asn Leu Ile Ala Ala Thr Ala Phe Met Glu

1130 1135 1140

Pro Pro Lys Glu Trp Met Tyr Ile Asp Lys Gly Gly Ala Glu Val

1145 1150 1155

Gly Pro Val Glu Lys Asp Val Ile Arg Ser Leu Trp Ser Lys Lys

1160 1165 1170

Asp Ile Asp Trp Thr Thr Lys Cys Arg Ala Leu Gly Met Ser Asp

1175 1180 1185

Trp Lys Lys Leu Arg Asp Ile Arg Glu Leu Arg Trp Ala Val Ala

1190 1195 1200

Val Arg Val Pro Val Leu Thr Pro Ser Gln Val Gly Asp Ala Ala

1205 1210 1215

Leu Ser Ile Leu His Ser Met Val Ser Ala His Ser Asp Leu Asp

1220 1225 1230

Asp Ala Gly Glu Ile Val Thr Pro Thr Pro Arg Val Lys Arg Ile

1235 1240 1245

Leu Ser Ser Thr Arg Cys Leu Pro His Ile Ala Gln Ala Leu Leu

1250 1255 1260

Ser Gly Glu Pro Val Ile Val Glu Ala Gly Ala Ala Leu Leu Lys

1265 1270 1275

Asp Val Val Thr Arg Asn Ser Lys Ala Met Ile Arg Leu Tyr Ser

1280 1285 1290

Thr Gly Ala Phe Tyr Phe Ala Leu Ala Tyr Pro Gly Ser Asn Leu

1295 1300 1305

Tyr Ser Ile Ala Gln Leu Phe Ser Val Thr His Val His Gln Ala

1310 1315 1320

Phe His Gly Gly Glu Glu Ala Thr Val Ser Ser Ser Leu Pro Leu

1325 1330 1335

Ala Lys Arg Ser Val Leu Gly Gly Leu Leu Pro Glu Ser Leu Leu

1340 1345 1350

Tyr Val Leu Glu Arg Ser Gly Pro Ala Ala Phe Ala Ala Gly Met

1355 1360 1365

Val Ser Asp Ser Asp Thr Pro Glu Ile Ile Trp Thr His Lys Met

1370 1375 1380

Arg Ala Glu Asn Leu Ile Cys Gln Val Leu Gln His Leu Gly Asp

1385 1390 1395

Tyr Pro Gln Lys Leu Ser Gln His Cys His Ser Leu Tyr Asp Tyr

1400 1405 1410

Ala Pro Met Pro Pro Val Thr Tyr Pro Glu Leu Arg Asp Glu Met

1415 1420 1425

Trp Cys His Arg Tyr Tyr Leu Arg Asn Leu Cys Asp Glu Ile Gln

1430 1435 1440

Phe Pro Asn Trp Pro Ile Val Glu His Val Glu Phe Leu Gln Ser

1445 1450 1455

Leu Leu Val Met Trp Arg Glu Glu Leu Thr Arg Lys Pro Met Asp

1460 1465 1470

Leu Ser Glu Gly Glu Ala Cys Lys Ile Leu Glu Ile Ser Leu Asn

1475 1480 1485

Asn Val Ser Ser Asp Asp Leu Asn Arg Thr Ala Ser Val Glu Leu

1490 1495 1500

Asn Glu Glu Ile Ser Asn Ile Ser Lys Gln Ile Gln Asn Leu Asp

1505 1510 1515

Glu Glu Lys Leu Lys Arg Gln Tyr Arg Lys Leu Ala Met Arg Tyr

1520 1525 1530

His Pro Asp Lys Asn Pro Glu Gly Arg Glu Lys Phe Leu Ala Val

1535 1540 1545

Gln Lys Ala Tyr Glu Cys Leu Gln Ala Thr Met Gln Gly Leu Gln

1550 1555 1560

Gly Pro Gln Pro Trp Arg Leu Leu Leu Leu Leu Lys Ala Gln Cys

1565 1570 1575

Ile Leu Tyr Arg Arg Tyr Gly His Val Leu Arg Pro Phe Lys Tyr

1580 1585 1590

Ala Gly Tyr Pro Met Leu Leu Asp Ala Val Thr Val Asp Lys Asp

1595 1600 1605

Asp Asn Asn Phe Leu Ser Asn Asp Arg Ser Pro Leu Leu Val Ala

1610 1615 1620

Ala Ser Glu Leu Val Ser Leu Thr Cys Ala Ala Ser Ser Leu Asn

1625 1630 1635

Gly Glu Glu Leu Val Arg Asp Gly Gly Val Gln Leu Leu Ser Thr

1640 1645 1650

Leu Leu Ser Arg Cys Met Cys Val Val Gln Pro Thr Thr Ser Gln

1655 1660 1665

His Glu Pro Ala Ala Ile Ile Val Thr Asn Val Met Arg Thr Leu

1670 1675 1680

Ser Val Ile Ser Gln Phe Glu Ser Ala Arg Ala Gly Phe Leu Glu

1685 1690 1695

Leu Pro Ser Leu Ile Glu Asp Ile Val His Cys Thr Glu Leu Glu

1700 1705 1710

Arg Val Pro Ala Ala Val Asp Ala Ala Leu Gln Ser Ile Ala Lys

1715 1720 1725

Val Ser Val Phe Pro Glu Leu Gln His Gly Leu Leu Lys Ala Gly

1730 1735 1740

Ala Leu Trp Tyr Ile Leu Pro Leu Leu Leu Gln Tyr Asp Ser Thr

1745 1750 1755

Ala Glu Glu Ser Asn Ser Val Glu Ser His Gly Val Gly Val Ser

1760 1765 1770

Ile Gln Ile Ala Lys Asn Glu His Ala Leu Gln Ala Ser Gln Ala

1775 1780 1785

Leu Ser Arg Leu Thr Gly Leu Cys Ala Asp Glu Ser Leu Thr Pro

1790 1795 1800

Tyr Asn Ala Thr Ala Ala Asp Val Leu Lys Ala Leu Leu Thr Pro

1805 1810 1815

Lys Leu Ala Ser Leu Leu Lys Asp Glu Val Ala Lys Asp Leu Leu

1820 1825 1830

Ser Lys Leu Asn Thr Asn Leu Glu Thr Pro Glu Ile Ile Trp Asn

1835 1840 1845

Ser Ala Thr Arg Ser Glu Leu Leu Asn Phe Val Asp Glu Gln Arg

1850 1855 1860

Ala Cys Gln Cys Pro Asp Gly Ser Tyr Asp Leu Lys Asn Ala Gln

1865 1870 1875

Ser Phe Ser Tyr Asp Ala Leu Ser Lys Glu Val Phe Val Gly Asn

1880 1885 1890

Val Tyr Leu Lys Val Tyr Asn Asp Gln Pro Asp Ser Glu Ile Ser

1895 1900 1905

Glu Pro Glu Ser Phe Cys Asn Ala Leu Ile Asp Phe Ile Ser Ser

1910 1915 1920

Leu Val His Thr Glu Leu Pro Ser Val Ser Glu Asp Gln Asn Leu

1925 1930 1935

Ile Glu Asp Arg Asn Ser Ser Asn Asp Thr Pro Glu Leu Gln Ser

1940 1945 1950

Ser Val Ala Glu Pro Ser Leu Ile Glu Glu His Ser Asp His Gln

1955 1960 1965

Pro Ser Ser Glu Gly Met Lys Asn Glu Glu Cys Phe Leu Ile Asp

1970 1975 1980

His Leu Gln Leu Gly Leu Thr Ala Leu Gln Asn Leu Leu Thr Lys

1985 1990 1995

Tyr Pro Asp Leu Ala Ser Val Phe Ser Ser Lys Glu Arg Leu Leu

2000 2005 2010

Pro Leu Phe Glu Cys Phe Ser Val Ala Ile Ala Ser Lys Thr Asp

2015 2020 2025

Ile Pro Lys Leu Cys Leu Asn Val Leu Ser Arg Leu Thr Ala Tyr

2030 2035 2040

Ala Pro Cys Leu Glu Thr Met Val Ser Asp Gly Ser Ser Leu Leu

2045 2050 2055

Leu Leu Leu Gln Met Leu His Ser Ala Pro Ser Phe Arg Glu Gly

2060 2065 2070

Ala Leu His Val Leu Tyr Ala Leu Ala Ser Thr Pro Glu Leu Ala

2075 2080 2085

Trp Ala Ala Ala Lys His Glu Glu Ile Pro Leu Gln Gln Arg Ala

2090 2095 2100

Ala Ala Ala Ser Leu Leu Gly Lys Leu Val Ala Gln Pro Met His

2105 2110 2115

Gly Pro Arg Val Ala Ile Thr Leu Val Arg Phe Leu Pro Asp Gly

2120 2125 2130

Leu Val Ser Ile Ile Arg Asp Gly Pro Gly Glu Ala Val Val His

2135 2140 2145

Ala Leu Glu Arg Thr Thr Glu Thr Pro Glu Leu Val Trp Thr Pro

2150 2155 2160

Ala Met Ala Ala Ser Leu Ser Ala Gln Ile Ala Thr Met Ala Ser

2165 2170 2175

Asp Ile Tyr Arg Glu Gln Gln Lys Gly Ser Val Ile Glu Trp Asp

2180 2185 2190

Val Pro Glu Gln Ser Ala Gly Gln Gln Glu Met Arg Asp Glu Pro

2195 2200 2205

Gln Val Gly Gly Ile Tyr Val Arg Arg Phe Leu Lys Asp Pro Lys

2210 2215 2220

Phe Pro Leu Arg Asn Pro Lys Arg Phe Leu Glu Gly Leu Leu Asp

2225 2230 2235

Gln Tyr Leu Ser Ala Met Ala Ala Thr His Tyr Glu Gln His Pro

2240 2245 2250

Val Asp Pro Glu Leu Pro Leu Leu Leu Ser Ala Ala Leu Val Ser

2255 2260 2265

Leu Leu Arg Val His Pro Ala Leu Ala Asp His Ile Gly His Leu

2270 2275 2280

Gly Tyr Val Pro Lys Leu Val Ala Ala Val Ala Tyr Glu Gly Arg

2285 2290 2295

Arg Glu Thr Met Ser Ser Gly Glu Val Lys Ala Glu Glu Ile Gly

2300 2305 2310

Ser Asp Gly Val Asn Glu Ser Thr Asp Pro Ser Ser Leu Pro Gly

2315 2320 2325

Gln Thr Pro Gln Glu Arg Val Arg Leu Ser Cys Leu Arg Val Leu

2330 2335 2340

His Gln Leu Ala Ala Ser Thr Thr Cys Ala Glu Ala Met Ala Ala

2345 2350 2355

Thr Ser Ala Gly Asn Ala Gln Val Val Pro Leu Leu Met Lys Ala

2360 2365 2370

Ile Gly Trp Leu Gly Gly Ser Ile Leu Ala Leu Glu Thr Leu Lys

2375 2380 2385

Arg Val Val Val Ala Gly Asn Arg Ala Arg Asp Ala Leu Val Ala

2390 2395 2400

Gln Gly Leu Lys Val Gly Leu Ile Glu Val Leu Leu Gly Leu Leu

2405 2410 2415

Asp Trp Arg Thr Gly Gly Arg Tyr Gly Leu Ser Set His Met Lys

2420 2425 2430

Trp Asn Glu Ser Glu Ala Ser Ile Gly Arg Val Leu Ala Val Glu

2435 2440 2445

Val Ser Val Glu Phe Val Ser Glu Met Phe Val Met Cys Val Thr

2450 2455 2460

His Val Leu His Gly Phe Ala Thr Glu Gly Ala His Cys Ser Lys

2465 2470 2475

Val Arg Glu Ile Leu Asp Ala Ser Glu Val Trp Ser Ala Tyr Lys

2480 2485 2490

Asp Gln Lys His Asp Leu Phe Leu Pro Ser Asn Thr Gln Ser Ala

2495 2500 2505

Ala Gly Val Ala Gly Phe Ile Glu Asn Ser Ser Asn Ser Leu Thr

2510 2515 2520

Tyr Ala Leu Thr Ala Pro Pro Pro Pro Ser His Pro

2525 2530 2535

<210>29

<211>3774

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(3774)

<400>29

atg gat caa gca aaa ggt tat gaa cat gtt cgg tat act gcc cct gac 48

Met Asp Gln Ala Lys Gly Tyr Glu His Val Arg Tyr Thr Ala Pro Asp

1 5 10 15

cct aga gat gag gga ctt ggc tcc att aat caa agg ttt tcc cac gac 96

Pro Arg Asp Glu Gly Leu Gly Ser Ile Asn Gln Arg Phe Ser His Asp

20 25 30

tct tca act aat gtt aac act tat gta cga cct cca gat tat ggt gtt 144

Ser Ser Thr Asn Val Asn Thr Tyr Val Arg Pro Pro Asp Tyr Gly Val

35 40 45

tca acc cct gct cgg cca gtg cta aac tac tca ata cag acc ggt gaa 192

Ser Thr Pro Ala Arg Pro Val Leu Asn Tyr Ser Ile Gln Thr Gly Glu

50 55 60

gaa ttt gct ttt gag ttt atg aga gat agg gtt att atg aaa ccg cag 240

Glu Phe Ala Phe Glu Phe Met Arg Asp Arg Val Ile Met Lys Pro Gln

65 70 75 80

ttc atc cca aat gtg tat ggt gag cac agt ggt atg cct gtt tct gtt 288

Phe Ile Pro Asn Val Tyr Gly Glu His Ser Gly Met Pro Val Ser Val

85 90 95

aac tta agt gct ctg gga atg gtt cat cca atg tca gag agt ggc cct 336

Asn Leu Ser Ala Leu Gly Met Val His Pro Met Ser Glu Ser Gly Pro

100 105 110

aac gct aca gtg ctt aac ata gaa gaa aaa cgt cag agc ttt gag cac 384

Asn Ala Thr Val Leu Asn Ile Glu Glu Lys Arg Gln Ser Phe Glu His

115 120 125

gag agg aaa ccc cct tct aga att gaa gat aag acc tat cat gaa ctg 432

Glu Arg Lys Pro Pro Ser Arg Ile Glu Asp Lys Thr Tyr His Glu Leu

130 135 140

gtc cag tca gcc cca gtt atc tct tcg aaa aat gat act ggt caa agg 480

Val Gln Ser Ala Pro Val Ile Ser Ser Lys Asn Asp Thr Gly Gln Arg

145 150 155 160

cgt cat agt ttg gtt tct tct aga gct tct gat agc tct ttg aac cgt 528

Arg His Ser Leu Val Ser Ser Arg Ala Ser Asp Ser Ser Leu Asn Arg

165 170 175

gcg aag ttc ttg tgt agt ttt ggt ggt aaa gtt ata ccc cgc ccc aga 576

Ala Lys Phe Leu Cys Ser Phe Gly Gly Lys Val Ile Pro Arg Pro Arg

180 185 190

gat cag aaa ctt agg tat gta ggt ggt gaa acg cgt atc ata cgg att 624

Asp Gln Lys Leu Arg Tyr Val Gly Gly Glu Thr Arg Ile Ile Arg Ile

195 200 205

agc aag act att tct ttc caa gaa ctc atg cat aaa atg aaa gaa ata 672

Ser Lys Thr Ile Ser Phe Gln Glu Leu Met His Lys Met Lys Glu Ile

210 215 220

ttt cct gaa gca cgc acc ata aaa tat cag ctg cca gga gag gat ctt 720

Phe Pro Glu Ala Arg Thr Ile Lys Tyr Gln Leu Pro Gly Glu Asp Leu

225 230 235 240

gat gcc cta gtc tct gta tct tct gac gag gat tta caa aac atg atg 768

Asp Ala Leu Val Ser Val Ser Ser Asp Glu Asp Leu Gln Asn Met Met

245 250 255

gaa gaa tgt atc gtg ttt ggt aat gga gga tct gag aag ccc agg atg 816

Glu Glu Cys Ile Val Phe Gly Asn Gly Gly Ser Glu Lys Pro Arg Met

260 265 270

ttc ttg ttt tca agc agt gat ata gag gag gct cag ttt gtt atg gaa 864

Phe Leu Phe Ser Ser Ser Asp Ile Glu Glu Ala Gln Phe Val Met Glu

275 280 285

cat gca gag ggt gat tct gag gtt cag tat gtt gtt gct gtc aat ggg 912

His Ala Glu Gly Asp Ser Glu Val Gln Tyr Val Val Ala Val Asn Gly

290 295 300

atg gat cta agt tca cgg aga agt tcc ctt gga tta agt cct ccc ggg 960

Met Asp Leu Ser Ser Arg Arg Ser Ser Leu Gly Leu Ser Pro Pro Gly

305 310 315 320

aac aat ttg gat gaa cta ctt cat ggg aat ttt gat agg aag atc gat 1008

Asn Asn Leu Asp Glu Leu Leu His Gly Asn Phe Asp Arg Lys Ile Asp

325 330 335

cgg gct gct aca gaa cca gca gtg gct tcg ctt act ccc tta gca ggt 1056

Arg Ala Ala Thr Glu Pro Ala Val Ala Ser Leu Thr Pro Leu Ala Gly

340 345 350

aat gaa tct tta cca gcg agc caa act tct caa cct gta aca gga ttt 1104

Asn Glu Ser Leu Pro Ala Ser Gln Thr Ser Gln Pro Val Thr Gly Phe

355 360 365

tct act gga aat gag cca ttt tca cag cct tat cta gga caa caa ttg 1152

Ser Thr Gly Asn Glu Pro Phe Ser Gln Pro Tyr Leu Gly Gln Gln Leu

370 375 380

cag ttc ccc gga ctt ggt aac cac caa att tac acg tca ggt cac atg 1200

Gln Phe Pro Gly Leu Gly Asn His Gln Ile Tyr Thr Ser Gly His Met

385 390 395 400

gca agc ata ggc tat ata gat gag aag agg tct gct cct tta cat gtt 1248

Ala Ser Ile Gly Tyr Ile Asp Glu Lys Arg Ser Ala Pro Leu His Val

405 410 415

caa cca caa cct cat tat atc ccg tat tct gtg aat cct gaa aca cct 1296

Gln Pro Gln Pro His Tyr Ile Pro Tyr Ser Val Asn Pro Glu Thr Pro

420 425 430

ctt gaa agc ctg gtg ccc cac tat cca caa aaa cct gag caa gga ttt 1344

Leu Glu Ser Leu Val Pro His Tyr Pro Gln Lys Pro Glu Gln Gly Phe

435 440 445

ttg cgt gag gag cag atc ttt cat gta caa gat cca gaa act tca tca 1392

Leu Arg Glu Glu Gln Ile Phe His Val Gln Asp Pro Glu Thr Ser Ser

450 455 460

aaa gag gcc aaa atg aga aga gat gac tca ttt cag aag gta aat gat 1440

Lys Glu Ala Lys Met Arg Arg Asp Asp Ser Phe Gln Lys Val Asn Asp

465 470 475 480

cat cct ata tct act gtc gag agc aat ctt tca gca aag gag cca aag 1488

His Pro Ile Ser Thr Val Glu Ser Asn Leu Ser Ala Lys Glu Pro Lys

485 490 495

atg agg aga gaa tcc tca acc cca agg gtc aat gag tat cct gtt tct 1536

Met Arg Arg Glu Ser Ser Thr Pro Arg Val Asn Glu Tyr Pro Val Ser

500 505 510

tct atg cct agt gat tta ata gtc cca gat gac ctc ccg aag gaa gaa 1584

Ser Met Pro Ser Asp Leu Ile Val Pro Asp Asp Leu Pro Lys Glu Glu

515 520 525

gct cca att gtc aca caa aca tct agt tca aca cca gat cca agt tct 1632

Ala Pro Ile Val Thr Gln Thr Ser Ser Ser Thr Pro Asp Pro Ser Ser

530 535 540

tca act ctc tca gag aaa agt ctt agg aaa tcc gag gac cat gtt gag 1680

Ser Thr Leu Ser Glu Lys Ser Leu Arg Lys Ser Glu Asp His Val Glu

545 550 555 560

aac aat ctg tca gca aag gag cca aag atg aga aaa gaa cac tcc acc 1728

Asn Asn Leu Ser Ala Lys Glu Pro Lys Met Arg Lys Glu His Ser Thr

565 570 575

aca agg gtc aat gaa tat tcc gtt tcc tct gta tct agt gat tct atg 1776

Thr Arg Val Asn Glu Tyr Ser Val Ser Ser Val Ser Ser Asp Ser Met

580 585 590

gtc cca gat caa gcc ctc aag gaa gaa gct cct att tcc atg aag ata 1824

Val Pro Asp Gln Ala Leu Lys Glu Glu Ala Pro Ile Ser Met Lys Ile

595 600 605

tcc aat tca aca cca gat cca aaa tcc ttg gtt tat cca gaa aaa agt 1872

Ser Asn Ser Thr Pro Asp Pro Lys Ser Leu Val Tyr Pro Glu Lys Ser

610 615 620

ctt aga aca tcc cag gag aaa acg ggt gcc ttc gat aca aca aat gaa 1920

Leu Arg Thr Ser Gln Glu Lys Thr Gly Ala Phe Asp Thr Thr Asn Glu

625 630 635 640

ggc atg aaa aag aat cag gac aat caa ttt tgt ctg ctt gga gga ttc 1968

Gly Met Lys Lys Asn Gln Asp Asn Gln Phe Cys Leu Leu Gly Gly Phe

645 650 655

tca gta tct gga cat ggt act tca aat aat agt tca tct aat gtg agc 2016

Ser Val Ser Gly His Gly Thr Ser Asn Asn Ser Ser Ser Asn Val Ser

660 665 670

aat ttc gac cag cct gtg act cag caa aga gtc ttt cat tct gag cga 2064

Asn Phe Asp Gln Pro Val Thr Gln Gln Arg Val Phe His Ser Glu Arg

675 680 685

act gta cga gat cca aca gaa act aac cgt ttg tct aaa tct gat gat 2112

Thr Val Arg Asp Pro Thr Glu Thr Asn Arg Leu Ser Lys Ser Asp Asp

690 695 700

tcc ctt gct tct caa ttt gta atg gct caa aca aca tca gat gct ttc 2160

Ser Leu Ala Ser Gln Phe Val Met Ala Gln Thr Thr Ser Asp Ala Phe

705 710 715 720

ctg cct atc agc gaa tca tct gaa act tct cat gaa gca aat atg gag 2208

Leu Pro Ile Ser Glu Ser Ser Glu Thr Ser His Glu Ala Asn Met Glu

725 730 735

tcc cag aat gtt cat cct act gcg cca gta ata cca gct cct gat agc 2256

Ser Gln Asn Val His Pro Thr Ala Pro Val Ile Pro Ala Pro Asp Ser

740 745 750

atc tgg aca gcc gag ggt agt atg tca cag tct gaa aaa aaa aac gtg 2304

Ile Trp Thr Ala Glu Gly Ser Met Ser Gln Ser Glu Lys Lys Asn Val

755 760 765

gaa act aac acc ccg gag cat gta agt cag aca gag act tca gca aag 2352

Glu Thr Asn Thr Pro Glu His Val Ser Gln Thr Glu Thr Ser Ala Lys

770 775 780

gct gtt cca caa gga cac aat gag aag ggg gat ata gtt gtt gat ata 2400

Ala Val Pro Gln Gly His Asn Glu Lys Gly Asp Ile Val Val Asp Ile

785 790 795 800

aat gat agg ttt cct cgt gag ttt ctt gct gat ata tta aaa acg aaa 2448

Asn Asp Arg Phe Pro Arg Glu Phe Leu Ala Asp Ile Leu Lys Thr Lys

805 810 815

gag tct ctg aac ttc cct gga tta ggg cca ttg cat gcc gat gga gct 2496

Glu Ser Leu Asn Phe Pro Gly Leu Gly Pro Leu His Ala Asp Gly Ala

820 825 830

ggt gtg agt tta aat att cag aat aat gac cct aaa act tgg tcg tat 2544

Gly Val Ser Leu Asn Ile Gln Asn Asn Asp Pro Lys Thr Trp Ser Tyr

835 840 845

ttt cga aat ttg gcg cag gat gag ttt gag agg aag gat cta tcc ctt 2592

Phe Arg Asn Leu Ala Gln Asp Glu Phe Glu Arg Lys Asp Leu Ser Leu

850 855 860

atg gat cag gac cac cct gga ttt ccc act tcc atg act aac acc aac 2640

Met Asp Gln Asp His Pro Gly Phe Pro Thr Ser Met Thr Asn Thr Asn

865 870 875 880

gga gtt cct att gat tat agc tac cca cca ttg cag tct gag aaa gtt 2688

Gly Val Pro Ile Asp Tyr Ser Tyr Pro Pro Leu Gln Ser Glu Lys Val

885 890 895

gcc tca agt cag ata cat cca caa atc cac ttt gat gga aat atc aag 2736

Ala Ser Ser Gln Ile His Pro Gln Ile His Phe Asp Gly Asn Ile Lys

900 905 910

cca gat gtg tct acc att acc ata cct gat ttg aac aca gta gac aca 2784

Pro Asp Val Ser Thr Ile Thr Ile Pro Asp Leu Asn Thr Val Asp Thr

915 920 925

caa gaa gat tac agt cag tca caa atc aaa ggt gct gaa agc acg gat 2832

Gln Glu Asp Tyr Ser Gln Ser Gln Ile Lys Gly Ala Glu Ser Thr Asp

930 935 940

gca act ctg aat gct gga gtt cct ctt att gac ttt atg gct gcg gat 2880

Ala Thr Leu Asn Ala Gly Val Pro Leu Ile Asp Phe Met Ala Ala Asp

945 950 955 960

agt ggc atg agg tct ctg cag gtc att aaa aat gac gac ttg gaa gaa 2928

Ser Gly Met Arg Ser Leu Gln Val Ile Lys Asn Asp Asp Leu Glu Glu

965 970 975

ctg aag gaa tta ggt tct ggt act ttt gga act gtt tat cac gga aaa 2976

Leu Lys Glu Leu Gly Ser Gly Thr Phe Gly Thr Val Tyr His Gly Lys

980 985 990

tgg agg ggt aca gat gtt gct atc aag cga ata aaa agg agc tgt ttt 3024

Trp Arg Gly Thr Asp Val Ala Ile Lys Arg Ile Lys Arg Ser Cys Phe

995 1000 1005

att ggt cgt tca tct gaa caa gag aga ttg acc tcg gag ttc tgg 3069

Ile Gly Arg Ser Ser Glu Gln Glu Arg Leu Thr Ser Glu Phe Trp

1010 1015 1020

cat gaa gca gaa att ctt tca aag ctt cat cat cca aat gtt atg 3114

His Glu Ala Glu Ile Leu Ser Lys Leu His His Pro Asn Val Met

1025 1030 1035

gca ttt tac ggc gta gtg aaa gat gga cca gga gga act tta gct 3159

Ala Phe Tyr Gly Val Val Lys Asp Gly Pro Gly Gly Thr Leu Ala

1040 1045 1050

aca gtg aca gag tac atg gtc aat gga tcg ctc agg cat gtt ctg 3204

Thr Val Thr Glu Tyr Met Val Asn Gly Ser Leu Arg His Val Leu

1055 1060 1065

ctc agc aac agg cac ctt gat cga cgt aag cga ctt atc att gca 3249

Leu Ser Asn Arg His Leu Asp Arg Arg Lys Arg Leu Ile Ile Ala

1070 1075 1080

atg gac gca gct ttt ggg atg gaa tat ttg cac tca aag agc ata 3294

Met Asp Ala Ala Phe Gly Met Glu Tyr Leu His Ser Lys Ser Ile

1085 1090 1095

gtg cat ttc gat ttg aag tgt gat aac ttg ctt gtc aac tta aag 3339

Val His Phe Asp Leu Lys Cys Asp Asn Leu Leu Val Asn Leu Lys

1100 1105 1110

gat ccc gcc cgt ccc ata tgc aag gtt ggt gat ttt ggt ctg tca 3384

Asp Pro Ala Arg Pro Ile Cys Lys Val Gly Asp Phe Gly Leu Ser

1115 1120 1125

aag ata aaa aga aac act ttg gtc act ggc ggt gta agg gga acc 3429

Lys Ile Lys Arg Asn Thr Leu Val Thr Gly Gly Val Arg Gly Thr

1130 1135 1140

ctc cct tgg atg gct ccc gag cta ctt agt gga agc agc agc aaa 3474

Leu Pro Trp Met Ala Pro Glu Leu Leu Ser Gly Ser Ser Ser Lys

1145 1150 1155

gtt tct gaa aag gtt gat gtg ttc tct ttc gga att gtc tta tgg 3519

Val Ser Glu Lys Val Asp Val Phe Ser Phe Gly Ile Val Leu Trp

1160 1165 1170

gaa att ctt acc ggt gag gaa ccc tac gcc aat atg cat tat ggg 3564

Glu Ile Leu Thr Gly Glu Glu Pro Tyr Ala Asn Met His Tyr Gly

1175 1180 1185

gca ata atc gga ggc ata gtg aac aat aca ttg aga cca acc gtg 3609

Ala Ile Ile Gly Gly Ile Val Asn Asn Thr Leu Arg Pro Thr Val

1190 1195 1200

cca aac tac tgt gac ccg gag tgg aga atg ctg atg gag cag tgt 3654

Pro Asn Tyr Cys Asp Pro Glu Trp Arg Met Leu Met Glu Gln Cys

1205 1210 1215

tgg gct cct gac cca ttt gtt cga cct gcg ttc ccg gaa ata gcc 3699

Trp Ala Pro Asp Pro Phe Val Arg Pro Ala Phe Pro Glu Ile Ala

1220 1225 1230

aga cgt ctc cgc acc atg tcc tcc tct gcg gtc cac aca aaa cca 3744

Arg Arg Leu Arg Thr Met Ser Ser Ser Ala Val His Thr Lys Pro

1235 1240 1245

cac gct gtc aac cac caa atc cac aag taa 3774

His Ala Val Asn His Gln Ile His Lys

1250 1255

<210>30

<211>1257

<212>PRT

＜213〉Arabidopis thaliana

<400>30

Met Asp Gln Ala Lys Gly Tyr Glu His Val Arg Tyr Thr Ala Pro Asp

1 5 10 15

Pro Arg Asp Glu Gly Leu Gly Ser Ile Asn Gln Arg Phe Ser His Asp

20 25 30

Ser Ser Thr Asn Val Asn Thr Tyr Val Arg Pro Pro Asp Tyr Gly Val

35 40 45

Ser Thr Pro Ala Arg Pro Val Leu Asn Tyr Ser Ile Gln Thr Gly Glu

50 55 60

Glu Phe Ala Phe Glu Phe Met Arg Asp Arg Val Ile Met Lys Pro Gln

65 70 75 80

Phe Ile Pro Asn Val Tyr Gly Glu His Ser Gly Met Pro Val Ser Val

85 90 95

Asn Leu Ser Ala Leu Gly Met Val His Pro Met Ser Glu Ser Gly Pro

100 105 110

Asn Ala Thr Val Leu Asn Ile Glu Glu Lys Arg Gln Ser Phe Glu His

115 120 125

Glu Arg Lys Pro Pro Ser Arg Ile Glu Asp Lys Thr Tyr His Glu Leu

130 135 140

Val Gln Ser Ala Pro Val Ile Ser Ser Lys Asn Asp Thr Gly Gln Arg

145 150 155 160

Arg His Ser Leu Val Ser Ser Arg Ala Ser Asp Ser Ser Leu Asn Arg

165 170 175

Ala Lys Phe Leu Cys Ser Phe Gly Gly Lys Val Ile Pro Arg Pro Arg

180 185 190

Asp Gln Lys Leu Arg Tyr Val Gly Gly Glu Thr Arg Ile Ile Arg Ile

195 200 205

Ser Lys Thr Ile Ser Phe Gln Glu Leu Met His Lys Met Lys Glu Ile

210 215 220

Phe Pro Glu Ala Arg Thr Ile Lys Tyr Gln Leu Pro Gly Glu Asp Leu

225 230 235 240

Asp Ala Leu Val Ser Val Ser Ser Asp Glu Asp Leu Gln Asn Met Met

245 250 255

Glu Glu Cys Ile Val Phe Gly Asn Gly Gly Ser Glu Lys Pro Arg Met

260 265 270

Phe Leu Phe Ser Ser Ser Asp Ile Glu Glu Ala Gln Phe Val Met Glu

275 280 285

His Ala Glu Gly Asp Ser Glu Val Gln Tyr Val Val Ala Val Asn Gly

290 295 300

Met Asp Leu Ser Ser Arg Arg Ser Ser Leu Gly Leu Ser Pro Pro Gly

305 310 315 320

Asn Asn Leu Asp Glu Leu Leu His Gly Asn Phe Asp Arg Lys Ile Asp

325 330 335

Arg Ala Ala Thr Glu Pro Ala Val Ala Ser Leu Thr Pro Leu Ala Gly

340 345 350

Asn Glu Ser Leu Pro Ala Ser Gln Thr Ser Gln Pro Val Thr Gly Phe

355 360 365

Ser Thr Gly Asn Glu Pro Phe Ser Gln Pro Tyr Leu Gly Gln Gln Leu

370 375 380

Gln Phe Pro Gly Leu Gly Asn His Gln Ile Tyr Thr Ser Gly His Met

385 390 395 400

Ala Ser Ile Gly Tyr Ile Asp Glu Lys Arg Ser Ala Pro Leu His Val

405 410 415

Gln Pro Gln Pro His Tyr Ile Pro Tyr Ser Val Asn Pro Glu Thr Pro

420 425 430

Leu Glu Ser Leu Val Pro His Tyr Pro Gln Lys Pro Glu Gln Gly Phe

435 440 445

Leu Arg Glu Glu Gln Ile Phe His Val Gln Asp Pro Glu Thr Ser Ser

450 455 460

Lys Glu Ala Lys Met Arg Arg Asp Asp Ser Phe Gln Lys Val Asn Asp

465 470 475 480

His Pro Ile Ser Thr Val Glu Ser Asn Leu Ser Ala Lys Glu Pro Lys

485 490 495

Met Arg Arg Glu Ser Ser Thr Pro Arg Val Asn Glu Tyr Pro Val Ser

500 505 510

Ser Met Pro Ser Asp Leu Ile Val Pro Asp Asp Leu Pro Lys Glu Glu

515 520 525

Ala Pro Ile Val Thr Gln Thr Ser Ser Ser Thr Pro Asp Pro Ser Ser

530 535 540

Ser Thr Leu Ser Glu Lys Ser Leu Arg Lys Ser Glu Asp His Val Glu

545 550 555 560

Asn Asn Leu Ser Ala Lys Glu Pro Lys Met Arg Lys Glu His Ser Thr

565 570 575

Thr Arg Val Asn Glu Tyr Ser Val Ser Ser Val Ser Ser Asp Ser Met

580 585 590

Val Pro Asp Gln Ala Leu Lys Glu Glu Ala Pro Ile Ser Met Lys Ile

595 600 605

Ser Asn Ser Thr Pro Asp Pro Lys Ser Leu Val Tyr Pro Glu Lys Ser

610 615 620

Leu Arg Thr Ser Gln Glu Lys Thr Gly Ala Phe Asp Thr Thr Asn Glu

625 630 635 640

Gly Met Lys Lys Asn Gln Asp Asn Gln Phe Cys Leu Leu Gly Gly Phe

645 650 655

Ser Val Ser Gly His Gly Thr Ser Asn Asn Ser Ser Ser Asn Val Ser

660 665 670

Asn Phe Asp Gln Pro Val Thr Gln Gln Arg Val Phe His Ser Glu Arg

675 680 685

Thr Val Arg Asp Pro Thr Glu Thr Asn Arg Leu Ser Lys Ser Asp Asp

690 695 700

Ser Leu Ala Ser Gln Phe Val Met Ala Gln Thr Thr Ser Asp Ala Phe

705 710 715 720

Leu Pro Ile Ser Glu Ser Ser Glu Thr Ser His Glu Ala Asn Met Glu

725 730 735

Ser Gln Asn Val His Pro Thr Ala Pro Val Ile Pro Ala Pro Asp Ser

740 745 750

Ile Trp Thr Ala Glu Gly Ser Met Ser Gln Ser Glu Lys Lys Asn Val

755 760 765

Glu Thr Asn Thr Pro Glu His Val Ser Gln Thr Glu Thr Ser Ala Lys

770 775 780

Ala Val Pro Gln Gly His Asn Glu Lys Gly Asp Ile Val Val Asp Ile

785 790 795 800

Asn Asp Arg Phe Pro Arg Glu Phe Leu Ala Asp Ile Leu Lys Thr Lys

805 810 815

Glu Ser Leu Asn Phe Pro Gly Leu Gly Pro Leu His Ala Asp Gly Ala

820 825 830

Gly Val Ser Leu Asn Ile Gln Asn Asn Asp Pro Lys Thr Trp Ser Tyr

835 840 845

Phe Arg Asn Leu Ala Gln Asp Glu Phe Glu Arg Lys Asp Leu Ser Leu

850 855 860

Met Asp Gln Asp His Pro Gly Phe Pro Thr Ser Met Thr Asn Thr Asn

865 870 875 880

Gly Val Pro Ile Asp Tyr Ser Tyr Pro Pro Leu Gln Ser Glu Lys Val

885 890 895

Ala Ser Ser Gln Ile His Pro Gln Ile His Phe Asp Gly Asn Ile Lys

900 905 910

Pro Asp Val Ser Thr Ile Thr Ile Pro Asp Leu Asn Thr Val Asp Thr

915 920 925

Gln Glu Asp Tyr Ser Gln Ser Gln Ile Lys Gly Ala Glu Ser Thr Asp

930 935 940

Ala Thr Leu Asn Ala Gly Val Pro Leu Ile Asp Phe Met Ala Ala Asp

945 950 955 960

Ser Gly Met Arg Ser Leu Gln Val Ile Lys Asn Asp Asp Leu Glu Glu

965 970 975

Leu Lys Glu Leu Gly Ser Gly Thr Phe Gly Thr Val Tyr His Gly Lys

980 985 990

Trp Arg Gly Thr Asp Val Ala Ile Lys Arg Ile Lys Arg Ser Cys Phe

995 1000 1005

Ile Gly Arg Ser Ser Glu Gln Glu Arg Leu Thr Ser Glu Phe Trp

1010 1015 1020

His Glu Ala Glu Ile Leu Ser Lys Leu His His Pro Asn Val Met

1025 1030 1035

Ala Phe Tyr Gly Val Val Lys Asp Gly Pro Gly Gly Thr Leu Ala

1040 1045 1050

Thr Val Thr Glu Tyr Met Val Asn Gly Ser Leu Arg His Val Leu

1055 1060 1065

Leu Ser Asn Arg His Leu Asp Arg Arg Lys Arg Leu Ile Ile Ala

1070 1075 1080

Met Asp Ala Ala Phe Gly Met Glu Tyr Leu His Ser Lys Ser Ile

1085 1090 1095

Val His Phe Asp Leu Lys Cys Asp Asn Leu Leu Val Asn Leu Lys

1100 1105 1110

Asp Pro Ala Arg Pro Ile Cys Lys Val Gly Asp Phe Gly Leu Ser

1115 1120 1125

Lys Ile Lys Arg Asn Thr Leu Val Thr Gly Gly Val Arg Gly Thr

1130 1135 1140

Leu Pro Trp Met Ala Pro Glu Leu Leu Ser Gly Ser Ser Ser Lys

1145 1150 1155

Val Ser Glu Lys Val Asp Val Phe Ser Phe Gly Ile Val Leu Trp

1160 1165 1170

Glu Ile Leu Thr Gly Glu Glu Pro Tyr Ala Asn Met His Tyr Gly

1175 1180 1185

Ala Ile Ile Gly Gly Ile Val Asn Asn Thr Leu Arg Pro Thr Val

1190 1195 1200

Pro Asn Tyr Cys Asp Pro Glu Trp Arg Met Leu Met Glu Gln Cys

1205 1210 1215

Trp Ala Pro Asp Pro Phe Val Arg Pro Ala Phe Pro Glu Ile Ala

1220 1225 1230

Arg Arg Leu Arg Thr Met Ser Ser Ser Ala Val His Thr Lys Pro

1235 1240 1245

His Ala Val Asn His Gln Ile His Lys

1250 1255

<210>31

<211>966

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(966)

<400>31

atg gat ctc gct gca gaa gag ctt caa ttt ctc aac att caa ggc ata 48

Met Asp Leu Ala Ala Glu Glu Leu Gln Phe Leu Asn Ile Gln Gly Ile

1 5 10 15

tta cgt gaa tcc aca aca atc ccc aaa ttt tcc cca aaa acc ttc tac 96

Leu Arg Glu Ser Thr Thr Ile Pro Lys Phe Ser Pro Lys Thr Phe Tyr

20 25 30

ctc ata acc cta acc cta att ttc cct ctc tcc ttc gca atc ctc gct 144

Leu Ile Thr Leu Thr Leu Ile Phe Pro Leu Ser Phe Ala Ile Leu Ala

35 40 45

cat tcc ctc ttt acc caa cca atc tta gct cag cta gat gca act cct 192

His Ser Leu Phe Thr Gln Pro Ile Leu Ala Gln Leu Asp Ala Thr Pro

50 55 60

cct tca gat caa tct aaa acc aat cac gaa tgg acc ctt ctt ctc att 240

Pro Ser Asp Gln Ser Lys Thr Asn His Glu Trp Thr Leu Leu Leu Ile

65 70 75 80

tat caa ttc atc tac gtc atc ttc ctc ttc gcc ttc tct ctt ctc tca 288

Tyr Gln Phe Ile Tyr Val Ile Phe Leu Phe Ala Phe Ser Leu Leu Ser

85 90 95

aca gcc gcc gtc gtc ttc acc gtc gct tcg ctt tac acg gga aag cca 336

Thr Ala Ala Val Val Phe Thr Val Ala Ser Leu Tyr Thr Gly Lys Pro

100 105 110

gtt tct ttc tct tca aca atg tca gcg att cct ctg gtt ttg aag cgt 384

Val Ser Phe Ser Ser Thr Met Ser Ala Ile Pro Leu Val Leu Lys Arg

115 120 125

ttg ttc att acc ttc ctt tgg gtt tct tta atg atg ctt gtg tac aac 432

Leu Phe Ile Thr Phe Leu Trp Val Ser Leu Met Met Leu Val Tyr Asn

130 135 140

tct gtc ttc ttg ttg ttt ctc gtt gtt ttg atc gta gct atc gat tta 480

Ser Val Phe Leu Leu Phe Leu Val Val Leu Ile Val Ala Ile Asp Leu

145 150 155 160

caa agc gtg att ctc gct gtc ttc tcg atg gtt gtg att ttc gtt ctg 528

Gln Ser Val Ile Leu Ala Val Phe Ser Met Val Val Ile Phe Val Leu

165 170 175

ttt ttg ggt gtt cat gtt tac atg act gct tgg tgg cat tta gct agt 576

Phe Leu Gly Val His Val Tyr Met Thr Ala Trp Trp His Leu Ala Ser

180 185 190

gta gtc tct gtt ctt gaa ccg att tac ggt atc gcc gcg atg aag aag 624

Val Val Ser Val Leu Glu Pro Ile Tyr Gly Ile Ala Ala Met Lys Lys

195 200 205

agt tac gag ttg ctt aat gga aga act aat atg gcg tgt tcg atg gtt 672

Ser Tyr Glu Leu Leu Asn Gly Arg Thr Asn Met Ala Cys Ser Met Val

210 215 220

ttt atg tat ctt gct ctt tgt gga atc act gct ggt gtt ttc ggt ggt 720

Phe Met Tyr Leu Ala Leu Cys Gly Ile Thr Ala Gly Val Phe Gly Gly

225 230 235 240

gtt gtg gtt cat gga ggt gat gat ttc ggg ttg ttt acg aag att gtt 768

Val Val Val His Gly Gly Asp Asp Phe Gly Leu Phe Thr Lys Ile Val

245 250 255

gtt ggt ggt ttc ttg gtt ggg att cta gtg att gtg aat cta gtt ggg 816

Val Gly Gly Phe Leu Val Gly Ile Leu Val Ile Val Asn Leu Val Gly

260 265 270

ctg ctt gtg cag agt gtg ttt tac tat gtt tgt aag agt ttt cat cat 864

Leu Leu Val Gln Ser Val Phe Tyr Tyr Val Cys Lys Ser Phe His His

275 280 285

cag ccg att gat aaa tct gcg ttg cac gat cat ctt ggt gga tat ctt 912

Gln Pro Ile Asp Lys Ser Ala Leu His Asp His Leu Gly Gly Tyr Leu

290 295 300

ggt gac tat gtg ccg tta aag agc agt att cag atg gag aac ttt gat 960

Gly Asp Tyr Val Pro Leu Lys Ser Ser Ile Gln Met Glu Asn Phe Asp

305 310 315 320

att taa 966

Ile

<210>32

<211>321

<212>PRT

＜213〉Arabidopis thaliana

<400>32

Met Asp Leu Ala Ala Glu Glu Leu Gln Phe Leu Asn Ile Gln Gly Ile

1 5 10 15

Leu Arg Glu Ser Thr Thr Ile Pro Lys Phe Ser Pro Lys Thr Phe Tyr

20 25 30

Leu Ile Thr Leu Thr Leu Ile Phe Pro Leu Ser Phe Ala Ile Leu Ala

35 40 45

His Ser Leu Phe Thr Gln Pro Ile Leu Ala Gln Leu Asp Ala Thr Pro

50 55 60

Pro Ser Asp Gln Ser Lys Thr Asn His Glu Trp Thr Leu Leu Leu Ile

65 70 75 80

Tyr Gln Phe Ile Tyr Val Ile Phe Leu Phe Ala Phe Ser Leu Leu Ser

85 90 95

Thr Ala Ala Val Val Phe Thr Val Ala Ser Leu Tyr Thr Gly Lys Pro

100 105 110

Val Ser Phe Ser Ser Thr Met Ser Ala Ile Pro Leu Val Leu Lys Arg

115 120 125

Leu Phe Ile Thr Phe Leu Trp Val Ser Leu Met Met Leu Val Tyr Asn

130 135 140

Ser Val Phe Leu Leu Phe Leu Val Val Leu Ile Val Ala Ile Asp Leu

145 150 155 160

Gln Ser Val Ile Leu Ala Val Phe Ser Met Val Val Ile Phe Val Leu

165 170 175

Phe Leu Gly Val His Val Tyr Met Thr Ala Trp Trp His Leu Ala Ser

180 185 190

Val Val Ser Val Leu Glu Pro Ile Tyr Gly Ile Ala Ala Met Lys Lys

195 200 205

Ser Tyr Glu Leu Leu Asn Gly Arg Thr Asn Met Ala Cys Ser Met Val

210 215 220

Phe Met Tyr Leu Ala Leu Cys Gly Ile Thr Ala Gly Val Phe Gly Gly

225 230 235 240

Val Val Val His Gly Gly Asp Asp Phe Gly Leu Phe Thr Lys Ile Val

245 250 255

Val Gly Gly Phe Leu Val Gly Ile Leu Val Ile Val Asn Leu Val Gly

260 265 270

Leu Leu Val Gln Ser Val Phe Tyr Tyr Val Cys Lys Ser Phe His His

275 280 285

Gln Pro Ile Asp Lys Ser Ala Leu His Asp His Leu Gly Gly Tyr Leu

290 295 300

Gly Asp Tyr Val Pro Leu Lys Ser Ser Ile Gln Met Glu Asn Phe Asp

305 310 315 320

Ile

<210>33

<211>3513

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(3513)

<400>33

atg gca gca tct tcc gaa ata ctc ccg gag tcg tgg caa gtg ttc atc 48

Met Ala Ala Ser Ser Glu Ile Leu Pro Glu Ser Trp Gln Val Phe Ile

1 5 10 15

aat ttc cga gga gca gat ttg cgc aac ggt ttc atc agc cat ctg gcg 96

Asn Phe Arg Gly Ala Asp Leu Arg Asn Gly Phe Ile Ser His Leu Ala

20 25 30

gga gct ttg acc tca gct gga atc aca tac tac atc gac acg gaa gaa 144

Gly Ala Leu Thr Ser Ala Gly Ile Thr Tyr Tyr Ile Asp Thr Glu Glu

35 40 45

gtc ccg agc gaa gat ctc act gtc ctt ttc aag agg ata gag gaa tcg 192

Val Pro Ser Glu Asp Leu Thr Val Leu Phe Lys Arg Ile Glu Glu Ser

50 55 60

gaa atc gca ctg tcc atc ttc tcg agc aat tat gct gag tca aaa tgg 240

Glu Ile Ala Leu Ser Ile Phe Ser Ser Asn Tyr Ala Glu Ser Lys Trp

65 70 75 80

tgt ttg gac gag ctc gtg aag atc atg gaa caa gta aag aaa gga aag 288

Cys Leu Asp Glu Leu Val Lys Ile Met Glu Gln Val Lys Lys Gly Lys

85 90 95

ctc aga atc atg ccc gtc ttc ttc aac gtg aag cca gag gag gtg aga 336

Leu Arg Ile Met Pro Val Phe Phe Asn Val Lys Pro Glu Glu Val Arg

100 105 110

gag cag aac gga gag ttc gga ctt aag ctt tac gga gaa ggt aaa agc 384

Glu Gln Asn Gly Glu Phe Gly Leu Lys Leu Tyr Gly Glu Gly Lys Ser

115 120 125

aaa ega ccc aac ata cct aat tgg gag aac gct ttg cgg tct gtc cca 432

Lys Arg Pro Asn Ile Pro Asn Trp Glu Asn Ala Leu Arg Ser Val Pro

130 135 140

agc aag ata ggc ttg aat ttg gcg aat ttt aga aac gag aag gaa ctc 480

Ser Lys Ile Gly Leu Asn Leu Ala Asn Phe Arg Asn Glu Lys Glu Leu

145 150 155 160

ctt gac aag atc att gac tcc atc aaa aaa gta ctt gcc cga att aca 528

Leu Asp Lys Ile Ile Asp Ser Ile Lys Lys Val Leu Ala Arg Ile Thr

165 170 175

cga gca agc aga gta gca gaa tct cta aac ggg atc tca aaa gac tca 576

Arg Ala Ser Arg Val Ala Glu Ser Leu Asn Gly Ile Ser Lys Asp Ser

180 185 190

gag gca aag aat gta gac aca ttt tcg cca aac tcc agt gat ttt cca 624

Glu Ala Lys Asn Val Asp Thr Phe Ser Pro Asn Ser Ser Asp Phe Pro

195 200 205

tct act tcc att gac gac gac ctc agt atc aac tcg cct cag tac caa 672

Ser Thr Ser Ile Asp Asp Asp Leu Ser Ile Asn Ser Pro Gln Tyr Gln

210 215 220

gcc aca att ccc ccc gca agc agg gaa ggt gaa cgt ctc aac acg atc 720

Ala Thr Ile Pro Pro Ala Ser Arg Glu Gly Glu Arg Leu Asn Thr Ile

225 230 235 240

tct act gta agt tca act ggt agt att gaa cat cct cca ccc aac tac 768

Ser Thr Val Ser Ser Thr Gly Ser Ile Glu His Pro Pro Pro Asn Tyr

245 250 255

gga ata gaa cca cgc ctt aag gag atg gaa gaa aag tta gat ttt gat 816

Gly Ile Glu Pro Arg Leu Lys Glu Met Glu Glu Lys Leu Asp Phe Asp

260 265 270

agc ctc gaa act aaa act gtt gga att gtt ggg atg cct ggg att ggt 864

Ser Leu Glu Thr Lys Thr Val Gly Ile Val Gly Met Pro Gly Ile Gly

275 280 285

aaa acc act ctt gca gaa acg ttg tat aga aag tgg gaa cac aag ttt 912

Lys Thr Thr Leu Ala Glu Thr Leu Tyr Arg Lys Trp Glu His Lys Phe

290 295 300

gag agg agt atg ttt ttc cca gat gcc agt aag atg gcg aat gaa cac 960

Glu Arg Ser Met Phe Phe Pro Asp Ala Ser Lys Met Ala Asn Glu His

305 310 315 320

gga atg tgt tgg ctg cag aag aga tta ttg gaa gag ctg ttg aag gat 1008

Gly Met Cys Trp Leu Gln Lys Arg Leu Leu Glu Glu Leu Leu Lys Asp

325 330 335

act aat ctc aac ata gga tat aca acg aat gaa cat gag ttt tgt aag 1056

Thr Asn Leu Asn Ile Gly Tyr Thr Thr Asn Glu His Glu Phe Cys Lys

340 345 350

gat gtt ctt ctc cta aag aaa gtt ttt ctt gtc ata gat aat gtt agt 1104

Asp Val Leu Leu Leu Lys Lys Val Phe Leu Val Ile Asp Asn Val Ser

355 360 365

agc gag gaa cag atc gaa act ctt ttt ggt aaa tgg aat tgg att aaa 1152

Ser Glu Glu Gln Ile Glu Thr Leu Phe Gly Lys Trp Asn Trp Ile Lys

370 375 380

aat gga agc aag att gtt att acg tca agt gat gag tca atg ctc aag 1200

Asn Gly Ser Lys Ile Val Ile Thr Ser Ser Asp Glu Ser Met Leu Lys

385 390 395 400

ggt ttc gtt aaa gat act tat gta gtc cca agt ttg aac agc aga gac 1248

Gly Phe Val Lys Asp Thr Tyr Val Val Pro Ser Leu Asn Ser Arg Asp

405 410 415

agt cta ctg tgg ttt act aat cat gca ttt ggt ttg gat gat gcc cag 1296

Ser Leu Leu Trp Phe Thr Asn His Ala Phe Gly Leu Asp Asp Ala Gln

420 425 430

gga aac ttg gta aag ttg tcc aaa cac ttt ctg aat tat gcc aaa ggc 1344

Gly Asn Leu Val Lys Leu Ser Lys His Phe Leu Asn Tyr Ala Lys Gly

435 440 445

aac cca cta gcc ctc gga gct ttt ggt gta gaa ctt tgt ggg aaa gac 1392

Asn Pro Leu Ala Leu Gly Ala Phe Gly Val Glu Leu Cys Gly Lys Asp

450 455 460

aag gct gat tgg gaa aag aga ata aaa aca ttg aca cta att tcc aat 1440

Lys Ala Asp Trp Glu Lys Arg Ile Lys Thr Leu Thr Leu Ile Ser Asn

465 470 475 480

aag atg atc caa gat gtc ttg aga aga agg tat gat gaa ctc aca gag 1488

Lys Met Ile Gln Asp Val Leu Arg Arg Arg Tyr Asp Glu Leu Thr Glu

485 490 495

agg cag aaa gat att ttt ctt gac gtc gca tgt ttc ttc aaa tca gag 1536

Arg Gln Lys Asp Ile Phe Leu Asp Val Ala Cys Phe Phe Lys Ser Glu

500 505 510

aat gaa agt tat gta cga cac gtg gtg aat tca tgt gat tct gag tct 1584

Asn Glu Ser Tyr Val Arg His Val Val Asn Ser Cys Asp Ser Glu Ser

515 520 525

act aag agt tgg gat gaa ata aca gat ctc aaa gga aag ttt ctt gtc 1632

Thr Lys Ser Trp Asp Glu Ile Thr Asp Leu Lys Gly Lys Phe Leu Val

530 535 540

aat att tct ggt ggt cga gtt gag atg cat gat ata cta tgc aca ttc 1680

Asn Ile Ser Gly Gly Arg Val Glu Met His Asp Ile Leu Cys Thr Phe

545 550 555 560

gcc aag gaa ctt gct tca caa gca ttg act gaa gat aca agg gtt cat 1728

Ala Lys Glu Leu Ala Ser Gln Ala Leu Thr Glu Asp Thr Arg Val His

565 570 575

ctc agg ctg tgg aac tat caa gat atc atg tgg ttt ctc aac aat gaa 1776

Leu Arg Leu Trp Asn Tyr Gln Asp Ile Met Trp Phe Leu Asn Asn Glu

580 585 590

ttg gaa atg gaa aat gtc aga ggt att ttc tta gac atg tct aaa gtt 1824

Leu Glu Met Glu Asn Val Arg Gly Ile Phe Leu Asp Met Ser Lys Val

595 600 605

ccg gag gaa atg aca ttt gat ggt aac atc ttt agc aat atg tgc aat 1872

Pro Glu Glu Met Thr Phe Asp Gly Asn Ile Phe Ser Asn Met Cys Asn

610 615 620

ctt cga tat ctc aaa ata tac agt tct gtt tgc cat aag gaa ggc gaa 1920

Leu Arg Tyr Leu Lys Ile Tyr Ser Ser Val Cys His Lys Glu Gly Glu

625 630 635 640

ggt atc ttc aaa ttt gac aca gtt agg gaa att cag tta cca tta gac 1968

Gly Ile Phe Lys Phe Asp Thr Val Arg Glu Ile Gln Leu Pro Leu Asp

645 650 655

aag gta cgc tat ctc cac tgg atg aaa tat cca tgg gag aaa ctt cca 2016

Lys Val Arg Tyr Leu His Trp Met Lys Tyr Pro Trp Glu Lys Leu Pro

660 665 670

tca gac ttc aac ccg gag aat ctc gtt gat ctt gaa ctg cct tat agc 2064

Ser Asp Phe Asn Pro Glu Asn Leu Val Asp Leu Glu Leu Pro Tyr Ser

675 680 685

tcc att aag aaa gtt tgg gag ggt gtt aag gat acc ccg ata cta aag 2112

Ser Ile Lys Lys Val Trp Glu Gly Val Lys Asp Thr Pro Ile Leu Lys

690 695 700

tgg gcc aat cta agc tat tca agt aag ttg act aac ctt tta ggg ttg 2160

Trp Ala Asn Leu Ser Tyr Ser Ser Lys Leu Thr Asn Leu Leu Gly Leu

705 710 715 720

tca aat gct aaa aat ctt gaa aga ttg aat ctt gaa ggt tgc aca agt 2208

Ser Asn Ala Lys Asn Leu Glu Arg Leu Asn Leu Glu Gly Cys Thr Ser

725 730 735

ttg ctt aaa ctg ccc caa gag atg gag aac atg aaa agt ctt gtc ttc 2256

Leu Leu Lys Leu Pro Gln Glu Met Glu Asn Met Lys Ser Leu Val Phe

740 745 750

ctg aac atg aga cgt tgc act agt ctc aca tgt ctt caa agt att aaa 2304

Leu Asn Met Arg Arg Cys Thr Ser Leu Thr Cys Leu Gln Ser Ile Lys

755 760 765

gtg agc tct ctg aaa att ctc ata ctc agt gac tgc tca aaa ctt gag 2352

Val Ser Ser Leu Lys Ile Leu Ile Leu Ser Asp Cys Ser Lys Leu Glu

770 775 780

gaa ttt gag gtg att tcg gaa aat ctg gaa gaa tta tat tta gat gga 2400

Glu Phe Glu Val Ile Ser Glu Asn Leu Glu Glu Leu Tyr Leu Asp Gly

785 790 795 800

act gca ata aag gga ctt cct cca gcg gcc ggg gat ctg acg aga ctt 2448

Thr Ala Ile Lys Gly Leu Pro Pro Ala Ala Gly Asp Leu Thr Arg Leu

805 810 815

gtc gtc tta aat atg gaa ggc tgt aca gaa ctg gag agt ctt ccc aaa 2496

Val Val Leu Asn Met Glu Gly Cys Thr Glu Leu Glu Ser Leu Pro Lys

820 825 830

cgt ctt gga aaa cag aaa gct ctt caa gaa ctg gta ctc tct gga tgt 2544

Arg Leu Gly Lys Gln Lys Ala Leu Gln Glu Leu Val Leu Ser Gly Cys

835 840 845

tca aag ctc gag agc gtt cca acg gac gta aaa gac atg aaa cat cta 2592

Ser Lys Leu Glu Ser Val Pro Thr Asp Val Lys Asp Met Lys His Leu

850 855 860

cgg ctc tta ttg ctt gac ggc aca aga atc aga aag atc ccg aag ata 2640

Arg Leu Leu Leu Leu Asp Gly Thr Arg Ile Arg Lys Ile Pro Lys Ile

865 870 875 880

aag tcg cta aag tgt ttg tgc tta agt aga aat att gca atg gtc aat 2688

Lys Ser Leu Lys Cys Leu Cys Leu Ser Arg Asn Ile Ala Met Val Asn

885 890 895

cta caa gat aat ctc aaa gat ttc tct aat ctg aaa tgt ctt gtc atg 2736

Leu Gln Asp Asn Leu Lys Asp Phe Ser Asn Leu Lys Cys Leu Val Met

900 905 910

aag aac tgc gag aat ctc aga tat ctt cct tcg ctt cca aaa tgt ctt 2784

Lys Asn Cys Glu Asn Leu Arg Tyr Leu Pro Ser Leu Pro Lys Cys Leu

915 920 925

gag tac cta aac gta tat ggt tgt gaa aga cta gaa tca gtt gag aat 2832

Glu Tyr Leu Asn Val Tyr Gly Cys Glu Arg Leu Glu Ser Val Glu Asn

930 935 940

cca ctg gtt gct gat agg tta acg tta ttc ctt gat aga tct gag gaa 2880

Pro Leu Val Ala Asp Arg Leu Thr Leu Phe Leu Asp Arg Ser Glu Glu

945 950 955 960

tta cgt tcc act ttc ttg ttc act aat tgc cac aat ctg ttt caa gat 2928

Leu Arg Ser Thr Phe Leu Phe Thr Asn Cys His Asn Leu Phe Gln Asp

965 970 975

gca aag gac tca atc tca acc tac gcg aaa tgg aaa tgc cac cga ctt 2976

Ala Lys Asp Ser Ile Ser Thr Tyr Ala Lys Trp Lys Cys His Arg Leu

980 985 990

gca gtt gaa tgc tac gaa cag gac ata gtt tct gga gct ttt ttc aac 3024

Ala Val Glu Cys Tyr Glu Gln Asp Ile Val Ser Gly Ala Phe Phe Asn

995 1000 1005

act tgc tat cct gga tat ata gtc cct tcg tgg ttc gat cac caa 3069

Thr Cys Tyr Pro Gly Tyr Ile Val pro Ser Trp Phe Asp His Gln

1010 1015 1020

gca gtt gga tca gtc tta gag cca agg ctg gaa cca cat tgg tat 3114

Ala Val Gly Ser Val Leu Glu Pro Arg Leu Glu Pro His Trp Tyr

1025 1030 1035

aac act atg ctt tct ggg ata gct cta tgt gca gtt gta tca ttc 3159

Asn Thr Met Leu Ser Gly Ile Ala Leu Cys Ala Val Val Ser Phe

1040 1045 1050

cat gag aac caa gat ccg atc atc ggc agt ttc tca gta aaa tgc 3204

His Glu Asn Gln Asp Pro Ile Ile Gly Ser Phe Ser Val Lys Cys

1055 1060 1065

aca ttg caa ttt gaa aac gaa gat ggg tct ctt cgc ttt gat tgt 3249

Thr Leu Gln Phe Glu Asn Glu Asp Gly Ser Leu Arg Phe Asp Cys

1070 1075 1080

gat atc gga tgt ttg aac gaa cca gga atg att gag gca gac cat 3294

Asp Ile Gly Cys Leu Asn Glu Pro Gly Met Ile Glu Ala Asp His

1085 1090 1095

gtt ttt atc ggc tat gtc act tgc tca cgt ttg aaa gat cac cac 3339

Val Phe Ile Gly Tyr Val Thr Cys Ser Arg Leu Lys Asp His His

1100 1105 1110

tct ata cct att cat cac cct aca act gta aaa atg cag ttc cac 3384

Ser Ile Pro Ile His His Pro Thr Thr Val Lys Met Gln Phe His

1115 1120 1125

ttg act gat gct tgt aaa agt aaa gtg gtg gat tgt ggg ttc cgt 3429

Leu Thr Asp Ala Cys Lys Ser Lys Val Val Asp Cys Gly Phe Arg

1130 1135 1140

ttg atg tac acc cag agc cgt ggc tgt ttg tta gag gaa gaa gtc 3474

Leu Met Tyr Thr Gln Ser Arg Gly Cys Leu Leu Glu Glu Glu Val

1145 1150 1155

aac gcc aac ttc act aaa tta tac ttg ggt tta ttg taa 3513

Asn Ala Asn Phe Thr Lys Leu Tyr Leu Gly Leu Leu

1160 1165 1170

<210>34

<211>1170

<212>PRT

＜213〉Arabidopis thaliana

<400>34

Met Ala Ala Ser Ser Glu Ile Leu Pro Glu Ser Trp Gln Val Phe Ile

1 5 10 15

Asn Phe Arg Gly Ala Asp Leu Arg Asn Gly Phe Ile Ser His Leu Ala

20 25 30

Gly Ala Leu Thr Ser Ala Gly Ile Thr Tyr Tyr Ile Asp Thr Glu Glu

35 40 45

Val Pro Ser Glu Asp Leu Thr Val Leu Phe Lys Arg Ile Glu Glu Ser

50 55 60

Glu Ile Ala Leu Ser Ile Phe Ser Ser Asn Tyr Ala Glu Ser Lys Trp

65 70 75 80

Cys Leu Asp Glu Leu Val Lys Ile Met Glu Gln Val Lys Lys Gly Lys

85 90 95

Leu Arg Ile Met Pro Val Phe Phe Asn Val Lys Pro Glu Glu Val Arg

100 105 110

Glu Gln Asn Gly Glu Phe Gly Leu Lys Leu Tyr Gly Glu Gly Lys Ser

115 120 125

Lys Arg Pro Asn Ile Pro Asn Trp Glu Asn Ala Leu Arg Ser Val Pro

130 135 140

Ser Lys Ile Gly Leu Asn Leu Ala Asn Phe Arg Asn Glu Lys Glu Leu

145 150 155 160

Leu Asp Lys Ile Ile Asp Ser Ile Lys Lys Val Leu Ala Arg Ile Thr

165 170 175

Arg Ala Ser Arg Val Ala Glu Ser Leu Asn Gly Ile Ser Lys Asp Ser

180 185 190

Glu Ala Lys Asn Val Asp Thr Phe Ser Pro Asn Ser Ser Asp Phe Pro

195 200 205

Ser Thr Ser Ile Asp Asp Asp Leu Ser Ile Asn Ser Pro Gln Tyr Gln

210 215 220

Ala Thr Ile Pro Pro Ala Ser Arg Glu Gly Glu Arg Leu Asn Thr Ile

225 230 235 240

Ser Thr Val Ser Ser Thr Gly Ser Ile Glu His Pro Pro Pro Asn Tyr

245 250 255

Gly Ile Glu Pro Arg Leu Lys Glu Met Glu Glu Lys Leu Asp Phe Asp

260 265 270

Ser Leu Glu Thr Lys Thr Val Gly Ile Val Gly Met Pro Gly Ile Gly

275 280 285

Lys Thr Thr Leu Ala Glu Thr Leu Tyr Arg Lys Trp Glu His Lys Phe

290 295 300

Glu Arg Ser Met Phe Phe Pro Asp Ala Ser Lys Met Ala Asn Glu His

305 310 315 320

Gly Met Cys Trp Leu Gln Lys Arg Leu Leu Glu Glu Leu Leu Lys Asp

325 330 335

Thr Asn Leu Asn Ile Gly Tyr Thr Thr Asn Glu His Glu Phe Cys Lys

340 345 350

Asp Val Leu Leu Leu Lys Lys Val Phe Leu Val Ile Asp Asn Val Ser

355 360 365

Ser Glu Glu Gln Ile Glu Thr Leu Phe Gly Lys Trp Asn Trp Ile Lys

370 375 380

Asn Gly Ser Lys Ile Val Ile Thr Ser Ser Asp Glu Ser Met Leu Lys

385 390 395 400

Gly Phe Val Lys Asp Thr Tyr Val Val Pro Ser Leu Asn Ser Arg Asp

405 410 415

Ser Leu Leu Trp Phe Thr Asn His Ala Phe Gly Leu Asp Asp Ala Gln

420 425 430

Gly Asn Leu Val Lys Leu Ser Lys His Phe Leu Asn Tyr Ala Lys Gly

435 440 445

Asn Pro Leu Ala Leu Gly Ala Phe Gly Val Glu Leu Cys Gly Lys Asp

450 455 460

Lys Ala Asp Trp Glu Lys Arg Ile Lys Thr Leu Thr Leu Ile Ser Asn

465 470 475 480

Lys Met Ile Gln Asp Val Leu Arg Arg Arg Tyr Asp Glu Leu Thr Glu

485 490 495

Arg Gln Lys Asp Ile Phe Leu Asp Val Ala Cys Phe Phe Lys Ser Glu

500 505 510

Asn Glu Ser Tyr Val Arg His Val Val Asn Ser Cys Asp Ser Glu Ser

515 520 525

Thr Lys Ser Trp Asp Glu Ile Thr Asp Leu Lys Gly Lys Phe Leu Val

530 535 540

Asn Ile Ser Gly Gly Arg Val Glu Met His Asp Ile Leu Cys Thr Phe

545 550 555 560

Ala Lys Glu Leu Ala Ser Gln Ala Leu Thr Glu Asp Thr Arg Val His

565 570 575

Leu Arg Leu Trp Asn Tyr Gln Asp Ile Met Trp Phe Leu Asn Asn Glu

580 585 590

Leu Glu Met Glu Asn Val Arg Gly Ile Phe Leu Asp Met Ser Lys Val

595 600 605

Pro Glu Glu Met Thr Phe Asp Gly Asn Ile Phe Ser Asn Met Cys Asn

610 615 620

Leu Arg Tyr Leu Lys Ile Tyr Ser Ser Val Cys His Lys Glu Gly Glu

625 630 635 640

Gly Ile Phe Lys Phe Asp Thr Val Arg Glu Ile Gln Leu Pro Leu Asp

645 650 655

Lys Val Arg Tyr Leu His Trp Met Lys Tyr Pro Trp Glu Lys Leu Pro

660 665 670

Ser Asp Phe Asn Pro Glu Asn Leu Val Asp Leu Glu Leu Pro Tyr Ser

675 680 685

Ser Ile Lys Lys Val Trp Glu Gly Val Lys Asp Thr Pro Ile Leu Lys

690 695 700

Trp Ala Asn Leu Ser Tyr Ser Ser Lys Leu Thr Asn Leu Leu Gly Leu

705 710 715 720

Ser Asn Ala Lys Asn Leu Glu Arg Leu Asn Leu Glu Gly Cys Thr Ser

725 730 735

Leu Leu Lys Leu Pro Gln Glu Met Glu Asn Met Lys Ser Leu Val Phe

740 745 750

Leu Asn Met Arg Arg Cys Thr Ser Leu Thr Cys Leu Gln Ser Ile Lys

755 760 765

Val Ser Ser Leu Lys Ile Leu Ile Leu Ser Asp Cys Ser Lys Leu Glu

770 775 780

Glu Phe Glu Val Ile Ser Glu Asn Leu Glu Glu Leu Tyr Leu Asp Gly

785 790 795 800

Thr Ala Ile Lys Gly Leu Pro Pro Ala Ala Gly Asp Leu Thr Arg Leu

805 810 815

Val Val Leu Asn Met Glu Gly Cys Thr Glu Leu Glu Ser Leu Pro Lys

820 825 830

Arg Leu Gly Lys Gln Lys Ala Leu Gln Glu Leu Val Leu Ser Gly Cys

835 840 845

Ser Lys Leu Glu Ser Val Pro Thr Asp Val Lys Asp Met Lys His Leu

850 855 860

Arg Leu Leu Leu Leu Asp Gly Thr Arg Ile Arg Lys Ile Pro Lys Ile

865 870 875 880

Lys Ser Leu Lys Cys Leu Cys Leu Ser Arg Asn Ile Ala Met Val Asn

885 890 895

Leu Gln Asp Asn Leu Lys Asp Phe Ser Asn Leu Lys Cys Leu Val Met

900 905 910

Lys Asn Cys Glu Asn Leu Arg Tyr Leu Pro Ser Leu Pro Lys Cys Leu

915 920 925

Glu Tyr Leu Asn Val Tyr Gly Cys Glu Arg Leu Glu Ser Val Glu Asn

930 935 940

Pro Leu Val Ala Asp Arg Leu Thr Leu Phe Leu Asp Arg Ser Glu Glu

945 950 955 960

Leu Arg Ser Thr Phe Leu Phe Thr Asn Cys His Asn Leu Phe Gln Asp

965 970 975

Ala Lys Asp Ser Ile Ser Thr Tyr Ala Lys Trp Lys Cys His Arg Leu

980 985 990

Ala Val Glu Cys Tyr Glu Gln Asp Ile Val Ser Gly Ala Phe Phe Asn

995 1000 1005

Thr Cys Tyr Pro Gly Tyr Ile Val Pro Ser Trp Phe Asp His Gln

1010 1015 1020

Ala Val Gly Ser Val Leu Glu Pro Arg Leu Glu Pro His Trp Tyr

1025 1030 1035

Asn Thr Met Leu Ser Gly Ile Ala Leu Cys Ala Val Val Ser Phe

1040 1045 1050

His Glu Asn Gln Asp Pro Ile Ile Gly Ser Phe Ser Val Lys Cys

1055 1060 1065

Thr Leu Gln Phe Glu Asn Glu Asp Gly Ser Leu Arg Phe Asp Cys

1070 1075 1080

Asp Ile Gly Cys Leu Asn Glu Pro Gly Met Ile Glu Ala Asp His

1085 1090 1095

Val Phe Ile Gly Tyr Val Thr Cys Ser Arg Leu Lys Asp His His

1100 1105 1110

Ser Ile Pro Ile His His Pro Thr Thr Val Lys Met Gln Phe His

1115 1120 1125

Leu Thr Asp Ala Cys Lys Ser Lys Val Val Asp Cys Gly Phe Arg

1130 1135 1140

Leu Met Tyr Thr Gln Ser Arg Gly Cys Leu Leu Glu Glu Glu Val

1145 1150 1155

Asn Ala Asn Phe Thr Lys Leu Tyr Leu Gly Leu Leu

1160 1165 1170

<210>35

<211>780

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(780)

<400>35

atg gac cgg agg ctc aag aaa tgc tcg aca tcc acc gat gtt gaa tca 48

Met Asp Arg Arg Leu Lys Lys Cys Ser Thr Ser Thr Asp Val Glu Ser

1 5 10 15

gtt cat gat gtt agt aag gtc acg gat cct ttg cag aaa gct aag aga 96

Val His Asp Val Ser Lys Val Thr Asp Pro Leu Gln Lys Ala Lys Arg

20 25 30

gag ttg gat aat gtg gaa atc aaa gaa aaa cag aag aag cag aag aac 144

Glu Leu Asp Asn Val Glu Ile Lys Glu Lys Gln Lys Lys Gln Lys Asn

35 40 45

caa aat gaa aca tct gag aag gaa act aaa aaa ttc agc acc gtt tac 192

Gln Asn Glu Thr Ser Glu Lys Glu Thr Lys Lys Phe Ser Thr Val Tyr

50 55 60

gaa aag ttt aat gat act att aaa gaa cta gac agg gtt tct gga aca 240

Glu Lys Phe Asn Asp Thr Ile Lys Glu Leu Asp Arg Val Ser Gly Thr

65 70 75 80

tgt ccc ata cga cct gcc att cca ttc acg ccc cca aag gaa aag gtg 288

Cys Pro Ile Arg Pro Ala Ile Pro Phe Thr Pro Pro Lys Glu Lys Val

85 90 95

gaa ccg ata tat cac aat gag tgc aat ttc gat gat aaa gct cat ctg 336

Glu Pro Ile Tyr His Asn Glu Cys Asn Phe Asp Asp Lys Ala His Leu

100 105 110

gga gta tct gac agc gcc ctt ttt gta caa gga ttt gat act tcc cat 384

Gly Val Ser Asp Ser Ala Leu Phe Val Gln Gly Phe Asp Thr Ser His

115 120 125

cca agg cat gaa atc aag aca gca ttg tgg aat cat ttc tct tca tgt 432

Pro Arg His Glu Ile Lys Thr Ala Leu Trp Asn His Phe Ser Ser Cys

130 135 140

ggt aag gtc tat ctg att tat gtt ccc att gcg tgt tct acc ggt gct 480

Gly Lys Val Tyr Leu Ile Tyr Val Pro Ile Ala Cys Ser Thr Gly Ala

145 150 155 160

tcg gtg gga tat gct ttc att gat atg aaa aat gaa acc aag ggg ttg 528

Ser Val Gly Tyr Ala Phe Ile Asp Met Lys Asn Glu Thr Lys Gly Leu

165 170 175

aca ctc aat gga agt cat ttg gga gga cgg aag atc gat gtt atg ttc 576

Thr Leu Asn Gly Ser His Leu Gly Gly Arg Lys Ile Asp Val Met Phe

180 185 190

gcc ata gat aga gaa gag ttt tac ttc tct tct aac tta aaa cac tgt 624

Ala Ile Asp Arg Glu Glu Phe Tyr Phe Ser Ser Asn Leu Lys His Cys

195 200 205

caa cgc tgc cgt aat tat agg cca tgg ctt gtt tta aaa gcc atg tca 672

Gln Arg Cys Arg Asn Tyr Arg Pro Trp Leu Val Leu Lys Ala Met Ser

210 215 220

gat gcc tgc ttt gaa tat cac cag agg att aaa ccg cgg atc gtt ggc 720

Asp Ala Cys Phe Glu Tyr His Gln Arg Ile Lys Pro Arg Ile Val Gly

225 230 235 240

act ccc cat agc aag att ggt cgt ttt aca gcc att att ggt cgt cgc 768

Thr Pro His Ser Lys Ile Gly Arg Phe Thr Ala Ile Ile Gly Arg Arg

245 250 255

tct tac agc tag 780

Ser Tyr Ser

<210>36

<211>259

<212>PRT

＜213〉Arabidopis thaliana

<400>36

Met Asp Arg Arg Leu Lys Lys Cys Ser Thr Ser Thr Asp Val Glu Ser

1 5 10 15

Val His Asp Val Ser Lys Val Thr Asp Pro Leu Gln Lys Ala Lys Arg

20 25 30

Glu Leu Asp Asn Val Glu Ile Lys Glu Lys Gln Lys Lys Gln Lys Asn

35 40 45

Gln Asn Glu Thr Ser Glu Lys Glu Thr Lys Lys Phe Ser Thr Val Tyr

50 55 60

Glu Lys Phe Asn Asp Thr Ile Lys Glu Leu Asp Arg Val Ser Gly Thr

65 70 75 80

Cys Pro Ile Arg Pro Ala Ile Pro Phe Thr Pro Pro Lys Glu Lys Val

85 90 95

Glu Pro Ile Tyr His Asn Glu Cys Asn Phe Asp Asp Lys Ala His Leu

100 105 110

Gly Val Ser Asp Ser Ala Leu Phe Val Gln Gly Phe Asp Thr Ser His

115 120 125

Pro Arg His Glu Ile Lys Thr Ala Leu Trp Asn His Phe Ser Ser Cys

130 135 140

Gly Lys Val Tyr Leu Ile Tyr Val Pro Ile Ala Cys Ser Thr Gly Ala

145 150 155 160

Ser Val Gly Tyr Ala Phe Ile Asp Met Lys Asn Glu Thr Lys Gly Leu

165 170 175

Thr Leu Asn Gly Ser His Leu Gly Gly Arg Lys Ile Asp Val Met Phe

180 185 190

Ala Ile Asp Arg Glu Glu Phe Tyr Phe Ser Ser Asn Leu Lys His Cys

195 200 205

Gln Arg Cys Arg Asn Tyr Arg Pro Trp Leu Val Leu Lys Ala Met Ser

210 215 220

Asp Ala Cys Phe Glu Tyr His Gln Arg Ile Lys Pro Arg Ile Val Gly

225 230 235 240

Thr Pro His Ser Lys Ile Gly Arg Phe Thr Ala Ile Ile Gly Arg Arg

245 250 255

Ser Tyr Ser

<210>37

<211>1392

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(1392)

<400>37

atg atc tgt tca agt aaa gga aca atc tta atc gct aga ggt aaa aat 48

Met Ile Cys Ser Ser Lys Gly Thr Ile Leu Ile Ala Arg Gly Lys Asn

1 5 10 15

tca aat aca atc cct aga tta ccc cta aaa ggg tca tcc aca aat tac 96

Ser Asn Thr Ile Pro Arg Leu Pro Leu Lys Gly Ser Ser Thr Asn Tyr

20 25 30

gcg aat cga att caa att cga aac cca atc agt gtc aaa act ttc cgg 144

Ala Asn Arg Ile Gln Ile Arg Asn Pro Ile Ser Val Lys Thr Phe Arg

35 40 45

cga tca tct tca tcg tca atg gcg gtg gaa aca atg tcg atg gga tca 192

Arg Ser Ser Ser Ser Ser Met Ala Val Glu Thr Met Ser Met Gly Ser

50 55 60

gat tca tca act ttg att cta aca tca gga gca agc ggt cgc gtt agg 240

Asp Ser Ser Thr Leu Ile Leu Thr Ser Gly Ala Ser Gly Arg Val Arg

65 70 75 80

gta ctc ttc tcg atg cga gag ctt aag cgt ctc gtt acg att atc caa 288

Val Leu Phe Ser Met Arg Glu Leu Lys Arg Leu Val Thr Ile Ile Gln

85 90 95

tcg ttg att ctt ttc ctc ctc ctt ccg ttt cgc gtc gtc gtt tgg cgg 336

Ser Leu Ile Leu Phe Leu Leu Leu Pro Phe Arg Val Val Val Trp Arg

100 105 110

cgg agg act ggt gcg gtg gtt atc aga gac gat aag caa gag agg aag 384

Arg Arg Thr Gly Ala Val Val Ile Arg Asp Asp Lys Gln Glu Arg Lys

115 120 125

gtt tgg tct cct ccg cag atc gtg gtg agg aag agg aac atc ggt ggc 432

Val Trp Ser Pro Pro Gln Ile Val Val Arg Lys Arg Asn Ile Gly Gly

130 135 140

gaa agc agc gtt tct cct ccg tcg gtt cca gct gcg gtg gtg gat ggg 480

Glu Ser Ser Val Ser Pro Pro Ser Val Pro Ala Ala Val Val Asp Gly

145 150 155 160

gag gtt gct gtt cga cgt gaa ctg gcg att aag cga gtt ttg gag gat 528

Glu Val Ala Val Arg Arg Glu Leu Ala Ile Lys Arg Val Leu Glu Asp

165 170 175

gaa ggc ggc gat gga agc tcc gtc aga gat tat tcg cta ttc acg acg 576

Glu Gly Gly Asp Gly Ser Ser Val Arg Asp Tyr Ser Leu Phe Thr Thr

180 185 190

aag aga gga gat acg ttg ttt agt cag tca tgg tca cct ctt tcc cca 624

Lys Arg Gly Asp Thr Leu Phe Ser Gln Ser Trp Ser Pro Leu Ser Pro

195 200 205

aat cac agg gga ctt att gtt ctg cta cat gga tta aac gag cat agt 672

Asn His Arg Gly Leu Ile Val Leu Leu His Gly Leu Asn Glu His Ser

210 215 220

ggc agg tat agt gat ttt gca aag cag ctt aat gct aat ggg ttc aag 720

Gly Arg Tyr Ser Asp Phe Ala Lys Gln Leu Asn Ala Asn Gly Phe Lys

225 230 235 240

gtc tat gga att gac tgg atc ggt cat ggc gga agt gat gga ctt cat 768

Val Tyr Gly Ile Asp Trp Ile Gly His Gly Gly Ser Asp Gly Leu His

245 250 255

gct tac gtt cct tcc ctt gat tac gct gtc aca gat ttg aaa tca ttt 816

Ala Tyr Val Pro Ser Leu Asp Tyr Ala Val Thr Asp Leu Lys Ser Phe

260 265 270

ctt gaa aag gta ttc aca gag aat cca gga ctc ccc tgt ttc tgc ttt 864

Leu Glu Lys Val Phe Thr Glu Asn Pro Gly Leu Pro Cys Phe Cys Phe

275 280 285

gga cac tca aca ggt gga gca atc atc ctc aag gct atg ctg gat cca 912

Gly His Ser Thr Gly Gly Ala Ile Ile Leu Lys Ala Met Leu Asp Pro

290 295 300

aag att gaa tct cga gtt tca ggc att gca ttg act tca cca gct gtt 960

Lys Ile Glu Ser Arg Val Ser Gly Ile Ala Leu Thr Ser Pro Ala Val

305 310 315 320

gga gtc caa cca tcc cat cca atc ttc gct gtt ctt gct cca atc atg 1008

Gly Val Gln Pro Ser His Pro Ile Phe Ala Val Leu Ala Pro Ile Met

325 330 335

gcg ttt cta cta ccc agg tac caa atc agt gca gca aac aag aaa gga 1056

Ala Phe Leu Leu Pro Arg Tyr Gln Ile Ser Ala Ala Asn Lys Lys Gly

340 345 350

atg ccg gtt tct cgt gac cca gca gct ctc atc gcc aaa tac tct gac 1104

Met Pro Val Ser Arg Asp Pro Ala Ala Leu Ile Ala Lys Tyr Ser Asp

355 360 365

cca tta gtc ttc acc gga tcc atc cgg gtt aaa acc ggc tac gag atc 1152

Pro Leu Val Phe Thr Gly Ser Ile Arg Val Lys Thr Gly Tyr Glu Ile

370 375 380

ctt aga atc act gct cac ttg caa cag aac ctg aac aaa gtg aaa gtt 1200

Leu Arg Ile Thr Ala His Leu Gln Gln Asn Leu Asn Lys Val Lys Val

385 390 395 400

ccc ttt ctt gtg atg cac ggt act gac gac aca gtt acc gat cct agc 1248

Pro Phe Leu Val Met His Gly Thr Asp Asp Thr Val Thr Asp Pro Ser

405 410 415

gcc tca aag aag ctc tac gag gaa gct gcc tcg tca gac aaa tca ctc 1296

Ala Ser Lys Lys Leu Tyr Glu Glu Ala Ala Ser Ser Asp Lys Ser Leu

420 425 430

aag ctc tac gac ggg ttg ttg cac gat ctt ctt ttt gaa ccc gaa cga 1344

Lys Leu Tyr Asp Gly Leu Leu His Asp Leu Leu Phe Glu Pro Glu Arg

435 440 445

gaa atc atc gct gga gcc ata tta gat tgg cta aac cag cgg gtt tag 1392

Glu Ile Ile Ala Gly Ala Ile Leu Asp Trp Leu Asn Gln Arg Val

450 455 460

<210>38

<211>463

<212>PRT

＜213〉Arabidopis thaliana

<400>38

Met Ile Cys Ser Ser Lys Gly Thr Ile Leu Ile Ala Arg Gly Lys Asn

1 5 10 15

Ser Asn Thr Ile Pro Arg Leu Pro Leu Lys Gly Ser Ser Thr Asn Tyr

20 25 30

Ala Asn Arg Ile Gln Ile Arg Asn Pro Ile Ser Val Lys Thr Phe Arg

35 40 45

Arg Ser Ser Ser Ser Ser Met Ala Val Glu Thr Met Ser Met Gly Ser

50 55 60

Asp Ser Ser Thr Leu Ile Leu Thr Ser Gly Ala Ser Gly Arg Val Arg

65 70 75 80

Val Leu Phe Ser Met Arg Glu Leu Lys Arg Leu Val Thr Ile Ile Gln

85 90 95

Ser Leu Ile Leu Phe Leu Leu Leu Pro Phe Arg Val Val Val Trp Arg

100 105 110

Arg Arg Thr Gly Ala Val Val Ile Arg Asp Asp Lys Gln Glu Arg Lys

115 120 125

Val Trp Ser Pro Pro Gln Ile Val Val Arg Lys Arg Asn Ile Gly Gly

130 135 140

Glu Ser Ser Val Ser Pro Pro Ser Val Pro Ala Ala Val Val Asp Gly

145 150 155 160

Glu Val Ala Val Arg Arg Glu Leu Ala Ile Lys Arg Val Leu Glu Asp

165 170 175

Glu Gly Gly Asp Gly Ser Ser Val Arg Asp Tyr Ser Leu Phe Thr Thr

180 185 190

Lys Arg Gly Asp Thr Leu Phe Ser Gln Ser Trp Ser Pro Leu Ser Pro

195 200 205

Asn His Arg Gly Leu Ile Val Leu Leu His Gly Leu Asn Glu His Ser

210 215 220

Gly Arg Tyr Ser Asp Phe Ala Lys Gln Leu Asn Ala Asn Gly Phe Lys

225 230 235 240

Val Tyr Gly Ile Asp Trp Ile Gly His Gly Gly Ser Asp Gly Leu His

245 250 255

Ala Tyr Val Pro Ser Leu Asp Tyr Ala Val Thr Asp Leu Lys Ser Phe

260 265 270

Leu Glu Lys Val Phe Thr Glu Asn Pro Gly Leu Pro Cys Phe Cys Phe

275 280 285

Gly His Ser Thr Gly Gly Ala Ile Ile Leu Lys Ala Met Leu Asp Pro

290 295 300

Lys Ile Glu Ser Arg Val Ser Gly Ile Ala Leu Thr Ser Pro Ala Val

305 310 315 320

Gly Val Gln Pro Ser His Pro Ile Phe Ala Val Leu Ala Pro Ile Met

325 330 335

Ala Phe Leu Leu Pro Arg Tyr Gln Ile Ser Ala Ala Asn Lys Lys Gly

340 345 350

Met Pro Val Ser Arg Asp Pro Ala Ala Leu Ile Ala Lys Tyr Ser Asp

355 360 365

Pro Leu Val Phe Thr Gly Ser Ile Arg Val Lys Thr Gly Tyr Glu Ile

370 375 380

Leu Arg Ile Thr Ala His Leu Gln Gln Asn Leu Asn Lys Val Lys Val

385 390 395 400

Pro Phe Leu Val Met His Gly Thr Asp Asp Thr Val Thr Asp Pro Ser

405 410 415

Ala Ser Lys Lys Leu Tyr Glu Glu Ala Ala Ser Ser Asp Lys Ser Leu

420 425 430

Lys Leu Tyr Asp Gly Leu Leu His Asp Leu Leu Phe Glu Pro Glu Arg

435 440 445

Glu Ile Ile Ala Gly Ala Ile Leu Asp Trp Leu Asn Gln Arg Val

450 455 460

<210>39

<211>1401

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(1401)

<400>39

atg act gaa gtt ctt cac ttt cct tca tct cca agc gct tct cat tca 48

Met Thr Glu Val Leu His Phe Pro Ser Ser Pro Ser Ala Ser His Ser

1 5 10 15

tct tct tct tct tct tct tct cct tca cct tct tct tta tct tac gcc 96

Ser Ser Ser Ser Ser Ser Ser Pro Ser Pro Ser Ser Leu Ser Tyr Ala

20 25 30

tct cgc tct aat gcg act ctc ttg att agc tct gac cac aac cgg aga 144

Ser Arg Ser Asn Ala Thr Leu Leu Ile Ser Ser Asp His Asn Arg Arg

35 40 45

aac cca gtt gct aga ttc gat caa gat gtt gac ttt cat gcc tca atc 192

Asn Pro Val Ala Arg Phe Asp Gln Asp Val Asp Phe His Ala Ser Ile

50 55 60

gaa gaa caa gat ttg aga aga cgg agc agt acc gat gga gga gaa gaa 240

Glu Glu Gln Asp Leu Arg Arg Arg Ser Ser Thr Asp Gly Gly Glu Glu

65 70 75 80

gac gat ggt ggg gaa gat cag att tcg ttg ttg gct ctt ctt gtt gcc 288

Asp Asp Gly Gly Glu Asp Gln Ile Ser Leu Leu Ala Leu Leu Val Ala

85 90 95

att ttc agg aga tct ttg att tct tgc aag agt aac cgg agg gag ctt 336

Ile Phe Arg Arg Ser Leu Ile Ser Cys Lys Ser Asn Arg Arg Glu Leu

100 105 110

tgt agc atg gag att gga tgg cct acc aat gtc aga cac gtg gcg cac 384

Cys Ser Met Glu Ile Gly Trp Pro Thr Asn Val Arg His Val Ala His

115 120 125

gtt acc ttt gat cgt ttc aat ggc ttc ttg ggt ttg cct gtt gaa ttc 432

Val Thr Phe Asp Arg Phe Asn Gly Phe Leu Gly Leu Pro Val Glu Phe

130 135 140

gag cct gaa gtt cct aga aga gct cca agc gcc agt gca aca gtc ttt 480

Glu Pro Glu Val Pro Arg Arg Ala Pro Ser Ala Ser Ala Thr Val Phe

145 150 155 160

ggg gta tca acc gaa tca atg caa tta tcg tat gat tca aga ggc aat 528

Gly Val Ser Thr Glu Ser Met Gln Leu Ser Tyr Asp Ser Arg Gly Asn

165 170 175

tgt gta cca acc ata cta ttg ctg atg caa aac tgt tta tat agt caa 576

Cys Val Pro Thr Ile Leu Leu Leu Met Gln Asn Cys Leu Tyr Ser Gln

180 185 190

gga ggc ttg cag gca gag ggc att ttt aga ctc act gct gag aat agt 624

Gly Gly Leu Gln Ala Glu Gly Ile Phe Arg Leu Thr Ala Glu Asn Ser

195 200 205

gag gaa gag gcg gtt agg gaa caa tta aac cga gga ttt ata cct gag 672

Glu Glu Glu Ala Val Arg Glu Gln Leu Asn Arg Gly Phe Ile Pro Glu

210 215 220

cga atc gat gtt cac tgt ttg gca ggg ctt atc aag gca tgg ttt aga 720

Arg Ile Asp Val His Cys Leu Ala Gly Leu Ile Lys Ala Trp Phe Arg

225 230 235 240

gaa ctg ccg aca agc gtt ctt gat tcg ttg tcg cct gaa cag gtg atg 768

Glu Leu Pro Thr Ser Val Leu Asp Ser Leu Ser Pro Glu Gln Val Met

245 250 255

cag tgc caa aca gaa gag gaa aat gtt gag ctc gtt agg ctt ctt cca 816

Gln Cys Gln Thr Glu Glu Glu Asn Val Glu Leu Val Arg Leu Leu Pro

260 265 270

cct aca gaa gct gct cta ctt gat tgg gcc atc aat cta atg gca gat 864

Pro Thr Glu Ala Ala Leu Leu Asp Trp Ala Ile Asn Leu Met Ala Asp

275 280 285

gtt gtt cag tat gaa cat cta aac aag atg aat tca cgc aac atc gct 912

Val Val Gln Tyr Glu His Leu Asn Lys Met Asn Ser Arg Asn Ile Ala

290 295 300

atg gtt ttc gca cca aat atg aca cag atg gat gat cca ctg aca gca 960

Met Val Phe Ala Pro Asn Met Thr Gln Met Asp Asp Pro Leu Thr Ala

305 310 315 320

ctg atg tat gcg gtt caa gtg atg aac ttt ctc aag aca cta atc gaa 1008

Leu Met Tyr Ala Val Gln Val Met Asn Phe Leu Lys Thr Leu Ile Glu

325 330 335

aaa act tta aga gaa agg caa gac tca gtg gtc gag caa gct cat gca 1056

Lys Thr Leu Arg Glu Arg Gln Asp Ser Val Val Glu Gln Ala His Ala

340 345 350

ttc cct tta gaa ccg tct gat gag agt ggt cac caa agc cct tca caa 1104

Phe Pro Leu Glu Pro Ser Asp Glu Ser Gly His Gln Ser Pro Ser Gln

355 360 365

tct ttg gct ttt aac acc agt gag cag agt gaa gag acg caa tca gac 1152

Ser Leu Ala Phe Asn Thr Ser Glu Gln Ser Glu Glu Thr Gln Ser Asp

370 375 380

aac atc gaa aat gct gaa aat cag agt tca agc agt gag ata tca gac 1200

Asn Ile Glu Asn Ala Glu Asn Gln Ser Ser Ser Ser Glu Ile Ser Asp

385 390 395 400

gaa tta acc cta gag aac aat gca tgt gaa cag aga gaa aca gac ttt 1248

Glu Leu Thr Leu Glu Asn Asn Ala Cys Glu Gln Arg Glu Thr Asp Phe

405 410 415

gga aaa tac aga aca gga aga ttg agc gac tcg agt caa cag gtg gtg 1296

Gly Lys Tyr Arg Thr Gly Arg Leu Ser Asp Ser Ser Gln Gln Val Val

420 425 430

ctg aat cta gat cct cca gct cag tgg cca gtg ggc aga aca aag ggg 1344

Leu Asn Leu Asp Pro Pro Ala Gln Trp Pro Val Gly Arg Thr Lys Gly

435 440 445

ttg acc aac ttg agc cgt gta gga tcg agg gta gag cgt act gaa gct 1392

Leu Thr Asn Leu Ser Arg Val Gly Ser Arg Val Glu Arg Thr Glu Ala

450 455 460

tgg cgg tga 1401

Trp Arg

465

<210>40

<211>466

<212>PRT

＜213〉Arabidopis thaliana

<400>40

Met Thr Glu Val Leu His Phe Pro Ser Ser Pro Ser Ala Ser His Ser

1 5 10 15

Ser Ser Ser Ser Ser Ser Ser Pro Ser Pro Ser Ser Leu Ser Tyr Ala

20 25 30

Ser Arg Ser Asn Ala Thr Leu Leu Ile Ser Ser Asp His Asn Arg Arg

35 40 45

Asn Pro Val Ala Arg Phe Asp Gln Asp Val Asp Phe His Ala Ser Ile

50 55 60

Glu Glu Gln Asp Leu Arg Arg Arg Ser Ser Thr Asp Gly Gly Glu Glu

65 70 75 80

Asp Asp Gly Gly Glu Asp Gln Ile Ser Leu Leu Ala Leu Leu Val Ala

85 90 95

Ile Phe Arg Arg Ser Leu Ile Ser Cys Lys Ser Asn Arg Arg Glu Leu

100 105 110

Cys Ser Met Glu Ile Gly Trp Pro Thr Asn Val Arg His Val Ala His

115 120 125

Val Thr Phe Asp Arg Phe Asn Gly Phe Leu Gly Leu Pro Val Glu Phe

130 135 140

Glu Pro Glu Val Pro Arg Arg Ala Pro Ser Ala Ser Ala Thr Val Phe

145 150 155 160

Gly Val Ser Thr Glu Ser Met Gln Leu Ser Tyr Asp Ser Arg Gly Asn

165 170 175

Cys Val Pro Thr Ile Leu Leu Leu Met Gln Asn Cys Leu Tyr Ser Gln

180 185 190

Gly Gly Leu Gln Ala Glu Gly Ile Phe Arg Leu Thr Ala Glu Asn Ser

195 200 205

Glu Glu Glu Ala Val Arg Glu Gln Leu Asn Arg Gly Phe Ile Pro Glu

210 215 220

Arg Ile Asp Val His Cys Leu Ala Gly Leu Ile Lys Ala Trp Phe Arg

225 230 235 240

Glu Leu Pro Thr Ser Val Leu Asp Ser Leu Ser Pro Glu Gln Val Met

245 250 255

Gln Cys Gln Thr Glu Glu Glu Asn Val Glu Leu Val Arg Leu Leu Pro

260 265 270

Pro Thr Glu Ala Ala Leu Leu Asp Trp Ala Ile Asn Leu Met Ala Asp

275 280 285

Val Val Gln Tyr Glu His Leu Asn Lys Met Asn Ser Arg Asn Ile Ala

290 295 300

Met Val Phe Ala Pro Asn Met Thr Gln Met Asp Asp Pro Leu Thr Ala

305 310 315 320

Leu Met Tyr Ala Val Gln Val Met Asn Phe Leu Lys Thr Leu Ile Glu

325 330 335

Lys Thr Leu Arg Glu Arg Gln Asp Ser Val Val Glu Gln Ala His Ala

340 345 350

Phe Pro Leu Glu Pro Ser Asp Glu Ser Gly His Gln Ser Pro Ser Gln

355 360 365

Ser Leu Ala Phe Asn Thr Ser Glu Gln Ser Glu Glu Thr Gln Ser Asp

370 375 380

Asn Ile Glu Asn Ala Glu Asn Gln Ser Ser Ser Ser Glu Ile Ser Asp

385 390 395 400

Glu Leu Thr Leu Glu Asn Asn Ala Cys Glu Gln Arg Glu Thr Asp Phe

405 410 415

Gly Lys Tyr Arg Thr Gly Arg Leu Ser Asp Ser Ser Gln Gln Val Val

420 425 430

Leu Asn Leu Asp Pro Pro Ala Gln Trp Pro Val Gly Arg Thr Lys Gly

435 440 445

Leu Thr Asn Leu Ser Arg Val Gly Ser Arg Val Glu Arg Thr Glu Ala

450 455 460

Trp Arg

465

<210>41

<211>519

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(519)

<400>41

atg gcg aat caa gca gct gct gca gca ttc ttc ctt ttc gct tta gcc 48

Met Ala Asn Gln Ala Ala Ala Ala Ala Phe Phe Leu Phe Ala Leu Ala

1 5 10 15

gtc ttc tcc aac ttg gag ctc tca gct tct tca ctt gtc agt ggc aag 96

Val Phe Ser Asn Leu Glu Leu Ser Ala Ser Ser Leu Val Ser Gly Lys

20 25 30

atc tct tgc ctt gac tgc cac cgc gat ttc gac ttc tca ggc att aag 144

Ile Ser Cys Leu Asp Cys His Arg Asp Phe Asp Phe Ser Gly Ile Lys

35 40 45

gtc ctc ctt aaa tgc gac gga gag aag aaa caa ata acc gcg gtg gca 192

Val Leu Leu Lys Cys Asp Gly Glu Lys Lys Gln Ile Thr Ala Val Ala

50 55 60

gct gca gac gga tct ttc cgg tca gtg ctt cca acg gct gac aaa aaa 240

Ala Ala Asp Gly Ser Phe Arg Ser Val Leu Pro Thr Ala Asp Lys Lys

65 70 75 80

ggc tcc ata aat tgt ctt gca aag ctc ttg gga ggc cct gag caa ctc 288

Gly Ser Ile Asn Cys Leu Ala Lys Leu Leu Gly Gly Pro Glu Gln Leu

85 90 95

tat gct cac aaa cac aac ttg gtc tct gaa ttg gtc aaa tct aaa cac 336

Tyr Ala His Lys His Asn Leu Val Ser Glu Leu Val Lys Ser Lys His

100 105 110

gat tcc aaa gtt tta act acc tca aac cca ctt gcc ttc tct ctc tcc 384

Asp Ser Lys Val Leu Thr Thr Ser Asn Pro Leu Ala Phe Ser Leu Ser

115 120 125

tgc ccc aaa cca tcc cga gat gat atc gga agt atg atc gga gat tcc 432

Cys Pro Lys Pro Ser Arg Asp Asp Ile Gly Ser Met Ile Gly Asp Ser

130 135 140

aag act att aat ttt ccg ggg gca gga ggt ttt gga ttc cca cct gcc 480

Lys Thr Ile Asn Phe Pro Gly Ala Gly Gly Phe Gly Phe Pro Pro Ala

145 150 155 160

agc ttc ttt ccc ttc tta cca atc att ggt atc cca tga 519

Ser Phe Phe Pro Phe Leu Pro Ile Ile Gly Ile Pro

165 170

<210>42

<211>172

<212>PRT

＜213〉Arabidopis thaliana

<400>42

Met Ala Asn Gln Ala Ala Ala Ala Ala Phe Phe Leu Phe Ala Leu Ala

1 5 10 15

Val Phe Ser Asn Leu Glu Leu Ser Ala Ser Ser Leu Val Ser Gly Lys

20 25 30

Ile Ser Cys Leu Asp Cys His Arg Asp Phe Asp Phe Ser Gly Ile Lys

35 40 45

Val Leu Leu Lys Cys Asp Gly Glu Lys Lys Gln Ile Thr Ala Val Ala

50 55 60

Ala Ala Asp Gly Ser Phe Arg Ser Val Leu Pro Thr Ala Asp Lys Lys

65 70 75 80

Gly Ser Ile Asn Cys Leu Ala Lys Leu Leu Gly Gly Pro Glu Gln Leu

85 90 95

Tyr Ala His Lys His Asn Leu Val Ser Glu Leu Val Lys Ser Lys His

100 105 110

Asp Ser Lys Val Leu Thr Thr Ser Asn Pro Leu Ala Phe Ser Leu Ser

115 120 125

Cys Pro Lys Pro Ser Arg Asp Asp Ile Gly Ser Met Ile Gly Asp Ser

130 135 140

Lys Thr Ile Asn Phe Pro Gly Ala Gly Gly Phe Gly Phe Pro Pro Ala

145 150 155 160

Ser Phe Phe Pro Phe Leu Pro Ile Ile Gly Ile Pro

165 170

<210>43

<211>954

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(954)

<400>43

atg aaa agt gga ggg aac aca aac act aaa ctc ata ctt gtt cat cca 48

Met Lys Ser Gly Gly Asn Thr Asn Thr Lys Leu Ile Leu Val His Pro

1 5 10 15

tac att caa aag caa aca agc aca aat cgt cta tgg ctt ctc gct ttc 96

Tyr Ile Gln Lys Gln Thr Ser Thr Asn Arg Leu Trp Leu Leu Ala Phe

20 25 30

gtt tct ttc ttc aca atc gct ttt ctc cta act ctt ctc tac acc acc 144

Val Ser Phe Phe Thr Ile Ala Phe Leu Leu Thr Leu Leu Tyr Thr Thr

35 40 45

gac tcc atc atc tct tct aaa aac aac tcc gcc acc gtc tcc tcc gcc 192

Asp Ser Ile Ile Ser Ser Lys Asn Asn Ser Ala Thr Val Ser Ser Ala

50 55 60

gtc aat tct gcc gtc acc acc gct acc atc tct cag tta cca aca aca 240

Val Asn Ser Ala Val Thr Thr Ala Thr Ile Ser Gln Leu Pro Thr Thr

65 70 75 80

gcc atc aat gca atg ctt cac tac gct tca aga tca aac gac agc tac 288

Ala Ile Asn Ala Met Leu His Tyr Ala Ser Arg Ser Asn Asp Ser Tyr

85 90 95

cac atg tca tac gga gag atg aaa tca atc tcc gac gtc ctc cgc cgc 336

His Met Ser Tyr Gly Glu Met Lys Ser Ile Ser Asp Val Leu Arg Arg

100 105 110

tgc tct ccg ccg tgt aat ctc tta gtc ttc ggt ctt aca cac gaa acc 384

Cys Ser Pro Pro Cys Asn Leu Leu Val Phe Gly Leu Thr His Glu Thr

115 120 125

ctt ctc tgg aaa tcg cta aac cac aac ggg cgt aca gtt ttc atc gaa 432

Leu Leu Trp Lys Ser Leu Asn His Asn Gly Arg Thr Val Phe Ile Glu

130 135 140

gag aat cgt tac tac gct gct tac ttc gaa gaa atc cac ccg gag atc 480

Glu Asn Arg Tyr Tyr Ala Ala Tyr Phe Glu Glu Ile His Pro Glu Ile

145 150 155 160

gaa gtc ttc gat gtt cag tac acg acc aaa gct cgt gag gcg cgt gag 528

Glu Val Phe Asp Val Gln Tyr Thr Thr Lys Ala Arg Glu Ala Arg Glu

165 170 175

ctt gtg tcg gcg gtt aaa gaa gcg gcg agg aac gag tgt cgt cca gtg 576

Leu Val Ser Ala Val Lys Glu Ala Ala Arg Asn Glu Cys Arg Pro Val

180 185 190

cag aat ctt ctc ttt tca gat tgt aaa tta gga ctc aat gat ttg ccg 624

Gln Asn Leu Leu Phe Ser Asp Cys Lys Leu Gly Leu Asn Asp Leu Pro

195 200 205

aat cat gta tac gat gtt gat tgg gat gtg atc tta gtt gat gga cca 672

Asn His Val Tyr Asp Val Asp Trp Asp Val Ile Leu Val Asp Gly Pro

210 215 220

cgt ggc gac ggt gga gat gta ccg ggg agg atg tcg tcg att ttc acg 720

Arg Gly Asp Gly Gly Asp Val Pro Gly Arg Met Ser Ser Ile Phe Thr

225 230 235 240

gcg gcg gtt ctt gct cgg agt aaa aaa ggc ggg aat ccg aag acg cat 768

Ala Ala Val Leu Ala Arg Ser Lys Lys Gly Gly Asn Pro Lys Thr His

245 250 255

gtg ttt gtt cat gat tat tac aga gat gtt gag aga ctt tgt ggg gat 816

Val Phe Val His Asp Tyr Tyr Arg Asp Val Glu Arg Leu Cys Gly Asp

260 265 270

gag ttt ctt tgc cgg gag aat ctt gtg gaa tct aat gat ctg ctt gcg 864

Glu Phe Leu Cys Arg Glu Asn Leu Val Glu Ser Asn Asp Leu Leu Ala

275 280 285

cac tac gtg ttg gag aag atg gat aaa aac agc acg cag ttc tgt cgt 912

His Tyr Val Leu Glu Lys Met Asp Lys Asn Ser Thr Gln Phe Cys Arg

290 295 300

ggt cgt aag aag aaa cgc tct gtt tct tct cca tcg gct tga 954

Gly Arg Lys Lys Lys Arg Ser Val Ser Ser Pro Ser Ala

305 310 315

<210>44

<211>317

<212>PRT

＜213〉Arabidopis thaliana

<400>44

Met Lys Ser Gly Gly Asn Thr Asn Thr Lys Leu Ile Leu Val His Pro

1 5 10 15

Tyr Ile Gln Lys Gln Thr Ser Thr Asn Arg Leu Trp Leu Leu Ala Phe

20 25 30

Val Ser Phe Phe Thr Ile Ala Phe Leu Leu Thr Leu Leu Tyr Thr Thr

35 40 45

Asp Ser Ile Ile Ser Ser Lys Asn Asn Ser Ala Thr Val Ser Ser Ala

50 55 60

Val Asn Ser Ala Val Thr Thr Ala Thr Ile Ser Gln Leu Pro Thr Thr

65 70 75 80

Ala Ile Asn Ala Met Leu His Tyr Ala Ser Arg Ser Asn Asp Ser Tyr

85 90 95

His Met Ser Tyr Gly Glu Met Lys Ser Ile Ser Asp Val Leu Arg Arg

100 105 110

Cys Ser Pro Pro Cys Asn Leu Leu Val Phe Gly Leu Thr His Glu Thr

115 120 125

Leu Leu Trp Lys Ser Leu Asn His Asn Gly Arg Thr Val Phe Ile Glu

130 135 140

Glu Asn Arg Tyr Tyr Ala Ala Tyr Phe Glu Glu Ile His Pro Glu Ile

145 150 155 160

Glu Val Phe Asp Val Gln Tyr Thr Thr Lys Ala Arg Glu Ala Arg Glu

165 170 175

Leu Val Ser Ala Val Lys Glu Ala Ala Arg Asn Glu Cys Arg Pro Val

180 185 190

Gln Asn Leu Leu Phe Ser Asp Cys Lys Leu Gly Leu Asn Asp Leu Pro

195 200 205

Asn His Val Tyr Asp Val Asp Trp Asp Val Ile Leu Val Asp Gly Pro

210 215 220

Arg Gly Asp Gly Gly Asp Val Pro Gly Arg Met Ser Ser Ile Phe Thr

225 230 235 240

Ala Ala Val Leu Ala Arg Ser Lys Lys Gly Gly Asn Pro Lys Thr His

245 250 255

Val Phe Val His Asp Tyr Tyr Arg Asp Val Glu Arg Leu Cys Gly Asp

260 265 270

Glu Phe Leu Cys Arg Glu Asn Leu Val Glu Ser Asn Asp Leu Leu Ala

275 280 285

His Tyr Val Leu Glu Lys Met Asp Lys Asn Ser Thr Gln Phe Cys Arg

290 295 300

Gly Arg Lys Lys Lys Arg Ser Val Ser Ser Pro Ser Ala

305 310 315

<210>45

<211>1272

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(1272)

<400>45

atg aaa cta aat ctc tcg aat ctc aga ttt cta cgt acc cga aaa tcg 48

Met Lys Leu Asn Leu Ser Asn Leu Arg Phe Leu Arg Thr Arg Lys Ser

1 5 10 15

tta atc tcc cag acg cga gca atg act caa aat ccg gat cca aaa cct 96

Leu Ile Ser Gln Thr Arg Ala Met Thr Gln Asn Pro Asp Pro Lys Pro

20 25 30

gat cca tcg cag gtt cta gac gat atc ctc tgt tcg gag cag cgt gat 144

Asp Pro Ser Gln Val Leu Asp Asp Ile Leu Cys Ser Glu Gln Arg Asp

35 40 45

ggg cag att gag gaa aca gtc gac aca gcg ccg gcg agc ttg ggc tct 192

Gly Gln Ile Glu Glu Thr Val Asp Thr Ala Pro Ala Ser Leu Gly Ser

50 55 60

cca agt cgg gtc tta agc att gat act aga gta gag aga gct tgg gca 240

Pro Ser Arg Val Leu Ser Ile Asp Thr Arg Val Glu Arg Ala Trp Ala

65 70 75 80

cac tgg aaa aaa ctg ggt aga ccc aag tat atc gtt gct cca atg gtt 288

His Trp Lys Lys Leu Gly Arg Pro Lys Tyr Ile Val Ala Pro Met Val

85 90 95

gat aac tct gag ctt ccg ttt aga ttg ctc tgc cag aaa tac gga gct 336

Asp Asn Ser Glu Leu Pro Phe Arg Leu Leu Cys Gln Lys Tyr Gly Ala

100 105 110

cag gct gct tat act ccg atg ttg cat tct agg atc ttc acc gag act 384

Gln Ala Ala Tyr Thr Pro Met Leu His Ser Arg Ile Phe Thr Glu Thr

115 120 125

gag aag tat aga aat cag gag ttc acc acc tgt aag gag gac agg cca 432

Glu Lys Tyr Arg Asn Gln Glu Phe Thr Thr Cys Lys Glu Asp Arg Pro

130 135 140

ttg ttt gtg cag ttc tgt gct aat gat cct gat acg tta ttg gaa gct 480

Leu Phe Val Gln Phe Cys Ala Asn Asp Pro Asp Thr Leu Leu Glu Ala

145 150 155 160

gca aag aga gtc gaa cct tac tgc gac tat gtt gat atc aat tta ggg 528

Ala Lys Arg Val Glu Pro Tyr Cys Asp Tyr Val Asp Ile Asn Leu Gly

165 170 175

tgt cct cag cgt ata gcg agg cga gga aat tat ggt gca ttc ttg atg 576

Cys Pro Gln Arg Ile Ala Arg Arg Gly Asn Tyr Gly Ala Phe Leu Met

180 185 190

gat aat ctt cct ttg gtg aaa tca ctt gtt gaa aag tta gct cag aac 624

Asp Asn Leu Pro Leu Val Lys Ser Leu Val Glu Lys Leu Ala Gln Asn

195 200 205

ctc aat gtt cct gtc tcc tgt aaa atc cgg atc ttc ccg aac ctg gaa 672

Leu Asn Val Pro Val Ser Cys Lys Ile Arg Ile Phe Pro Asn Leu Glu

210 215 220

gat aca ctc aag tac gcc aag atg cta gaa gat gct ggt tgc tcg ctc 720

Asp Thr Leu Lys Tyr Ala Lys Met Leu Glu Asp Ala Gly Cys Ser Leu

225 230 235 240

cta gct gtt cac ggg cga aca aga gat gag aaa gac ggg aag aaa ttt 768

Leu Ala Val His Gly Arg Thr Arg Asp Glu Lys Asp Gly Lys Lys Phe

245 250 255

aga gct gat tgg agc gca atc aag gaa gtg aaa aac gct atg aga atc 816

Arg Ala Asp Trp Ser Ala Ile Lys Glu Val Lys Asn Ala Met Arg Ile

260 265 270

cct gtc tta gcg aat ggg aat gta aga tgc atc gaa gat gtc gat aac 864

Pro Val Leu Ala Asn Gly Asn Val Arg Cys Ile Glu Asp Val Asp Asn

275 280 285

tgc atc aaa gag acg ggt gtt gaa ggt gtt ctc tct gcg gag acg ctt 912

Cys Ile Lys Glu Thr Gly Val Glu Gly Val Leu Ser Ala Glu Thr Leu

290 295 300

ctt gaa aac ccg gcg gcc ttt gct ggg ttt aga aca gct gaa tgg gca 960

Leu Glu Asn Pro Ala Ala Phe Ala Gly Phe Arg Thr Ala Glu Trp Ala

305 310 315 320

aaa gat aac gaa gaa gag gga ttc gtc gat gga ggg tta gac cag gga 1008

Lys Asp Asn Glu Glu Glu Gly Phe Val Asp Gly Gly Leu Asp Gln Gly

325 330 335

gat tta gtt gtt gag tat tta aag ctg tgt gag aag cat ccg gtt cca 1056

Asp Leu Val Val Glu Tyr Leu Lys Leu Cys Glu Lys His Pro Val Pro

340 345 350

tgg agg atg att cga tct cac gtt cat aag atg ttg gga gaa tgg ttt 1104

Trp Arg Met Ile Arg Ser His Val His Lys Met Leu Gly Glu Trp Phe

355 360 365

aga att cat cca caa gtt aga gag caa ctt aat gct caa aac ata ttg 1152

Arg Ile His Pro Gln Val Arg Glu Gln Leu Asn Ala Gln Asn Ile Leu

370 375 380

acg ttt gag ttt cta tac gga ctt gtg gat cag cta aga gag ctt gga 1200

Thr Phe Glu Phe Leu Tyr Gly Leu Val Asp Gln Leu Arg Glu Leu Gly

385 390 395 400

gga aga gtt cca ctc tac aag aaa aag aag ata gat act ctg act cca 1248

Gly Arg Val Pro Leu Tyr Lys Lys Lys Lys Ile Asp Thr Leu Thr Pro

405 410 415

caa gac tct cca caa agg gtt tag 1272

Gln Asp Ser Pro Gln Arg Val

420

<210>46

<211>423

<212>PRT

＜213〉Arabidopis thaliana

<400>46

Met Lys Leu Asn Leu Ser Asn Leu Arg Phe Leu Arg Thr Arg Lys Ser

1 5 10 15

Leu Ile Ser Gln Thr Arg Ala Met Thr Gln Asn Pro Asp Pro Lys Pro

20 25 30

Asp Pro Ser Gln Val Leu Asp Asp Ile Leu Cys Ser Glu Gln Arg Asp

35 40 45

Gly Gln Ile Glu Glu Thr Val Asp Thr Ala Pro Ala Ser Leu Gly Ser

50 55 60

Pro Ser Arg Val Leu Ser Ile Asp Thr Arg Val Glu Arg Ala Trp Ala

65 70 75 80

His Trp Lys Lys Leu Gly Arg Pro Lys Tyr Ile Val Ala Pro Met Val

85 90 95

Asp Asn Ser Glu Leu Pro Phe Arg Leu Leu Cys Gln Lys Tyr Gly Ala

100 105 110

Gln Ala Ala Tyr Thr Pro Met Leu His Ser Arg Ile Phe Thr Glu Thr

115 120 125

Glu Lys Tyr Arg Asn Gln Glu Phe Thr Thr Cys Lys Glu Asp Arg Pro

130 135 140

Leu Phe Val Gln Phe Cys Ala Asn Asp Pro Asp Thr Leu Leu Glu Ala

145 150 155 160

Ala Lys Arg Val Glu Pro Tyr Cys Asp Tyr Val Asp Ile Asn Leu Gly

165 170 175

Cys Pro Gln Arg Ile Ala Arg Arg Gly Asn Tyr Gly Ala Phe Leu Met

180 185 190

Asp Asn Leu Pro Leu Val Lys Ser Leu Val Glu Lys Leu Ala Gln Asn

195 200 205

Leu Asn Val Pro Val Ser Cys Lys Ile Arg Ile Phe Pro Asn Leu Glu

210 215 220

Asp Thr Leu Lys Tyr Ala Lys Met Leu Glu Asp Ala Gly Cys Ser Leu

225 230 235 240

Leu Ala Val His Gly Arg Thr Arg Asp Glu Lys Asp Gly Lys Lys Phe

245 250 255

Arg Ala Asp Trp Ser Ala Ile Lys Glu Val Lys Asn Ala Met Arg Ile

260 265 270

Pro Val Leu Ala Asn Gly Asn Val Arg Cys Ile Glu Asp Val Asp Asn

275 280 285

Cys Ile Lys Glu Thr Gly Val Glu Gly Val Leu Ser Ala Glu Thr Leu

290 295 300

Leu Glu Asn Pro Ala Ala Phe Ala Gly Phe Arg Thr Ala Glu Trp Ala

305 310 315 320

Lys Asp Asn Glu Glu Glu Gly Phe Val Asp Gly Gly Leu Asp Gln Gly

325 330 335

Asp Leu Val Val Glu Tyr Leu Lys Leu Cys Glu Lys His Pro Val Pro

340 345 350

Trp Arg Met Ile Arg Ser His Val His Lys Met Leu Gly Glu Trp Phe

355 360 365

Arg Ile His Pro Gln Val Arg Glu Gln Leu Asn Ala Gln Asn Ile Leu

370 375 380

Thr Phe Glu Phe Leu Tyr Gly Leu Val Asp Gln Leu Arg Glu Leu Gly

385 390 395 400

Gly Arg Val Pro Leu Tyr Lys Lys Lys Lys Ile Asp Thr Leu Thr Pro

405 410 415

Gln Asp Ser Pro Gln Arg Val

420

<210>47

<211>777

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(777)

<400>47

atg gcg atg gcg gtc tcc gga gct gtc ctc agt ggg ctt ggt tct tcg 48

Met Ala Met Ala Val Ser Gly Ala Val Leu Ser Gly Leu Gly Ser Ser

1 5 10 15

ttc ctc acc gga ggc aag aga ggt gcc acc gca ttg gca agc ggc gta 96

Phe Leu Thr Gly Gly Lys Arg Gly Ala Thr Ala Leu Ala Ser Gly Val

20 25 30

ggc act gga gct cag aga gtt ggc agg aaa act ctt att gtc gct gct 144

Gly Thr Gly Ala Gln Arg Val Gly Arg Lys Thr Leu Ile Val Ala Ala

35 40 45

gcg gct gct cag cct aag aaa tct tgg atc cct gcc gtt aaa ggt ggt 192

Ala Ala Ala Gln Pro Lys Lys Ser Trp Ile Pro Ala Val Lys Gly Gly

50 55 60

ggc aac ttc ctt gac cct gaa tgg ctc gat ggc tcg cta cca gga gat 240

Gly Asn Phe Leu Asp Pro Glu Trp Leu Asp Gly Ser Leu Pro Gly Asp

65 70 75 80

ttc ggg ttc gac cca ttg ggt ttg ggg aaa gac ccg gct ttt ctg aaa 288

Phe Gly Phe Asp Pro Leu Gly Leu Gly Lys Asp Pro Ala Phe Leu Lys

85 90 95

tgg tac aga gag gct gag ctg atc cat ggc cga tgg gcg atg gca gcg 336

Trp Tyr Arg Glu Ala Glu Leu Ile His Gly Arg Trp Ala Met Ala Ala

100 105 110

gtt ctt ggg atc ttc gtc ggc cag gcc tgg agc ggt gtg gca tgg ttt 384

Val Leu Gly Ile Phe Val Gly Gln Ala Trp Ser Gly Val Ala Trp Phe

115 120 125

gaa gct gga gcc cag cca gac gcg atc gct ccc ttc tcg ttc ggg tcg 432

Glu Ala Gly Ala Gln Pro Asp Ala Ile Ala Pro Phe Ser Phe Gly Ser

130 135 140

ctt ctt gga acc caa ttg ctt ctc atg ggt tgg gtg gag agc aaa cga 480

Leu Leu Gly Thr Gln Leu Leu Leu Met Gly Trp Val Glu Ser Lys Arg

145 150 155 160

tgg gtc gat ttc ttc aac ccg gat tct caa tcg gtt gag tgg gca acg 528

Trp Val Asp Phe Phe Asn Pro Asp Ser Gln Ser Val Glu Trp Ala Thr

165 170 175

cca tgg tcg aag acc gcc gag aat ttc gcg aac tat acc ggc gat cag 576

Pro Trp Ser Lys Thr Ala Glu Asn Phe Ala Asn Tyr Thr Gly Asp Gln

180 185 190

gga tac ccc ggt ggg aga ttc ttc gat ccg ttg ggt ctc gcc ggg aaa 624

Gly Tyr Pro Gly Gly Arg Phe Phe Asp Pro Leu Gly Leu Ala Gly Lys

195 200 205

aac cgc gac ggt gtt tat gag ccg gac ttt gag aag ctg gag agg ctg 672

Asn Arg Asp Gly Val Tyr Glu Pro Asp Phe Glu Lys Leu Glu Arg Leu

210 215 220

aaa ttg gca gag att aag cac tcg agg ctc gca atg gtt gcc atg ttg 720

Lys Leu Ala Glu Ile Lys His Ser Arg Leu Ala Met Val Ala Met Leu

225 230 235 240

atc ttt tac ttt gag gcc ggg cag ggg aaa acg cct ctc ggt gct ctt 768

Ile Phe Tyr Phe Glu Ala Gly Gln Gly Lys Thr Pro Leu Gly Ala Leu

245 250 255

ggt ttg tga 777

Gly Leu

<210>48

<211>258

<212>PRT

＜213〉Arabidopis thaliana

<400>48

Met Ala Met Ala Val Ser Gly Ala Val Leu Ser Gly Leu Gly Ser Ser

1 5 10 15

Phe Leu Thr Gly Gly Lys Arg Gly Ala Thr Ala Leu Ala Ser Gly Val

20 25 30

Gly Thr Gly Ala Gln Arg Val Gly Arg Lys Thr Leu Ile Val Ala Ala

35 40 45

Ala Ala Ala Gln Pro Lys Lys Ser Trp Ile Pro Ala Val Lys Gly Gly

50 55 60

Gly Asn Phe Leu Asp Pro Glu Trp Leu Asp Gly Ser Leu Pro Gly Asp

65 70 75 80

Phe Gly Phe Asp Pro Leu Gly Leu Gly Lys Asp Pro Ala Phe Leu Lys

85 90 95

Trp Tyr Arg Glu Ala Glu Leu Ile His Gly Arg Trp Ala Met Ala Ala

100 105 110

Val Leu Gly Ile Phe Val Gly Gln Ala Trp Ser Gly Val Ala Trp Phe

115 120 125

Glu Ala Gly Ala Gln Pro Asp Ala Ile Ala Pro Phe Ser Phe Gly Ser

130 135 140

Leu Leu Gly Thr Gln Leu Leu Leu Met Gly Trp Val Glu Ser Lys Arg

145 150 155 160

Trp Val Asp Phe Phe Asn Pro Asp Ser Gln Ser Val Glu Trp Ala Thr

165 170 175

Pro Trp Ser Lys Thr Ala Glu Asn Phe Ala Asn Tyr Thr Gly Asp Gln

180 185 190

Gly Tyr Pro Gly Gly Arg Phe Phe Asp Pro Leu Gly Leu Ala Gly Lys

195 200 205

Asn Arg Asp Gly Val Tyr Glu Pro Asp Phe Glu Lys Leu Glu Arg Leu

210 215 220

Lys Leu Ala Glu Ile Lys His Ser Arg Leu Ala Met Val Ala Met Leu

225 230 235 240

Ile Phe Tyr Phe Glu Ala Gly Gln Gly Lys Thr Pro Leu Gly Ala Leu

245 250 255

Gly Leu

<210>49

<211>381

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(381)

<400>49

atg cct cca ccg gtt caa gat ttc ccg cca att ctc act cca ccg gct 48

Met Pro Pro Pro Val Gln Asp Phe Pro Pro Ile Leu Thr Pro Pro Ala

1 5 10 15

gaa gag ttc ccg ccg att ttg cct cca ccg gtt caa gag atc ccg ccg 96

Glu Glu Phe Pro Pro Ile Leu Pro Pro Pro Val Gln Glu Ile Pro Pro

20 25 30

gtt ttc aca tta cca ccg acc gta caa gat cca ccg aca att cca gta 144

Val Phe Thr Leu Pro Pro Thr Val Gln Asp Pro Pro Thr Ile Pro Val

35 40 45

ttc tcc aca cca cca gtc ctc gga gat ttc cca ccc caa act ccc gac 192

Phe Ser Thr Pro Pro Val Leu Gly Asp Phe Pro Pro Gln Thr Pro Asp

50 55 60

ttt acc acg ccg cca gag gtc aca aat cca tgg caa ccg ccg gtg acg 240

Phe Thr Thr Pro Pro Glu Val Thr Asn Pro Trp Gln Pro Pro Val Thr

65 70 75 80

tca ttc gca cca cca ata gag tcc atc cca aca ata ccg gat aat ccg 288

Ser Phe Ala Pro Pro Ile Glu Ser Ile Pro Thr Ile Pro Asp Asn Pro

85 90 95

ttt ccg gtt aca cca aac ccg gac atg ggt tca aat caa ccg ttt gtt 336

Phe Pro Val Thr Pro Asn Pro Asp Met Gly Ser Asn Gln Pro Phe Val

100 105 110

gag ctt cct ccg cct act tgg gat tcc ccg cca ttt aat cgt taa 381

Glu Leu Pro Pro Pro Thr Trp Asp Ser Pro Pro Phe Asn Arg

115 120 125

<210>50

<211>126

<212>PRT

＜213〉Arabidopis thaliana

<400>50

Met Pro Pro Pro Val Gln Asp Phe Pro Pro Ile Leu Thr Pro Pro Ala

1 5 10 15

Glu Glu Phe Pro Pro Ile Leu Pro Pro Pro Val Gln Glu Ile Pro Pro

20 25 30

Val Phe Thr Leu Pro Pro Thr Val Gln Asp Pro Pro Thr Ile Pro Val

35 40 45

Phe Ser Thr Pro Pro Val Leu Gly Asp Phe Pro Pro Gln Thr Pro Asp

50 55 60

Phe Thr Thr Pro Pro Glu Val Thr Asn Pro Trp Gln Pro Pro Val Thr

65 70 75 80

Ser Phe Ala Pro Pro Ile Glu Ser Ile Pro Thr Ile Pro Asp Asn Pro

85 90 95

Phe Pro Val Thr Pro Asn Pro Asp Met Gly Ser Asn Gln Pro Phe Val

100 105 110

Glu Leu Pro Pro Pro Thr Trp Asp Ser Pro Pro Phe Asn Arg

115 120 125

<210>51

<211>879

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(879)

<400>51

atg agt atg gag atg gag ttg ttt gtc act cca gag aag cag agg caa 48

Met Ser Met Glu Met Glu Leu Phe Val Thr Pro Glu Lys Gln Arg Gln

1 5 10 15

cat cct tca gtg agc gtt gag aaa act cca gtg aga agg aaa ttg att 96

His Pro Ser Val Ser Val Glu Lys Thr Pro Val Arg Arg Lys Leu Ile

20 25 30

gtt gat gat gat tct gaa att gga tca gag aag aaa ggg caa tca aga 144

Val Asp Asp Asp Ser Glu Ile Gly Ser Glu Lys Lys Gly Gln Ser Arg

35 40 45

act tct gga ggc ggg ctt cgt caa ttc agt gtt atg gtt tgt cag aag 192

Thr Ser Gly Gly Gly Leu Arg Gln Phe Ser Val Met Val Cys Gln Lys

50 55 60

ttg gaa gcc aag aag ata act act tac aag gag gtt gca gac gaa att 240

Leu Glu Ala Lys Lys Ile Thr Thr Tyr Lys Glu Val Ala Asp Glu Ile

65 70 75 80

att tca gat ttt gcc aca att aag caa aac gca gag aag cct ttg aat 288

Ile Ser Asp Phe Ala Thr Ile Lys Gln Asn Ala Glu Lys Pro Leu Asn

85 90 95

gaa aat gag tac aat gag aag aac ata agg cgg aga gtc tac gat gcg 336

Glu Asn Glu Tyr Asn Glu Lys Asn Ile Arg Arg Arg Val Tyr Asp Ala

100 105 110

ctc aat gtg ttc atg gcg ttg gat att att gca agg gat aaa aag gaa 384

Leu Asn Val Phe Met Ala Leu Asp Ile Ile Ala Arg Asp Lys Lys Glu

115 120 125

atc cgg tgg aaa gga ctt cct att acc tgc aaa aag gat gtg gaa gaa 432

Ile Arg Trp Lys Gly Leu Pro Ile Thr Cys Lys Lys Asp Val Glu Glu

130 135 140

gtc aag atg gat cgt aat aaa gtt atg agc agt gtg caa aag aag gct 480

Val Lys Met Asp Arg Asn Lys Val Met Ser Ser Val Gln Lys Lys Ala

145 150 155 160

gct ttt ctt aaa gag ttg aga gaa aag gtc tca agt ctt gag agt ctt 528

Ala Phe Leu Lys Glu Leu Arg Glu Lys Val Ser Ser Leu Glu Ser Leu

165 170 175

atg tcg aga aat caa gag atg gtt gtg aag act caa ggc cca gca gaa 576

Met Ser Arg Asn Gln Glu Met Val Val Lys Thr Gln Gly Pro Ala Glu

180 185 190

gga ttt acc tta cca ttc att cta ctt gag aca aac cct cac gca gta 624

Gly Phe Thr Leu Pro Phe Ile Leu Leu Glu Thr Asn Pro His Ala Val

195 200 205

gtc gaa atc gag att tct gaa gat atg caa ctt gta cac ctc gac ttc 672

Val Glu Ile Glu Ile Ser Glu Asp Met Gln Leu Val His Leu Asp Phe

210 215 220

aat agc aca cct ttc tcg gtc cat gat gat gct tac att ttg aaa ctg 720

Asn Ser Thr Pro Phe Ser Val His Asp Asp Ala Tyr Ile Leu Lys Leu

225 230 235 240

atg caa gaa cag aag caa gaa cag aac aga gta tct tct tct tca tct 768

Met Gln Glu Gln Lys Gln Glu Gln Asn Arg Val Ser Ser Ser Ser Ser

245 250 255

aca cat cac caa tct caa cat agc tcc gct cat tct tca tcc agt tct 816

Thr His His Gln Ser Gln His Ser Ser Ala His Ser Ser Ser Ser Ser

260 265 270

tgc att gct tct gga acc tca ggc ccg gtt tgc tgg aac tcg gga tcc 864

Cys Ile Ala Ser Gly Thr Ser Gly Pro Val Cys Trp Asn Ser Gly Ser

275 280 285

att gat act cgc tga 879

Ile Asp Thr Arg

290

<210>52

<211>292

<212>PRT

＜213〉Arabidopis thaliana

<400>52

Met Ser Met Glu Met Glu Leu Phe Val Thr Pro Glu Lys Gln Arg Gln

1 5 10 15

His Pro Ser Val Ser Val Glu Lys Thr Pro Val Arg Arg Lys Leu Ile

20 25 30

Val Asp Asp Asp Ser Glu Ile Gly Ser Glu Lys Lys Gly Gln Ser Arg

35 40 45

Thr Ser Gly Gly Gly Leu Arg Gln Phe Ser Val Met Val Cys Gln Lys

50 55 60

Leu Glu Ala Lys Lys Ile Thr Thr Tyr Lys Glu Val Ala Asp Glu Ile

65 70 75 80

Ile Ser Asp Phe Ala Thr Ile Lys Gln Asn Ala Glu Lys Pro Leu Asn

85 90 95

Glu Asn Glu Tyr Asn Glu Lys Asn Ile Arg Arg Arg Val Tyr Asp Ala

100 105 110

Leu Asn Val Phe Met Ala Leu Asp Ile Ile Ala Arg Asp Lys Lys Glu

115 120 125

Ile Arg Trp Lys Gly Leu Pro Ile Thr Cys Lys Lys Asp Val Glu Glu

130 135 140

Val Lys Met Asp Arg Asn Lys Val Met Ser Ser Val Gln Lys Lys Ala

145 150 155 160

Ala Phe Leu Lys Glu Leu Arg Glu Lys Val Ser Ser Leu Glu Ser Leu

165 170 175

Met Ser Arg Asn Gln Glu Met Val Val Lys Thr Gln Gly Pro Ala Glu

180 185 190

Gly Phe Thr Leu Pro Phe Ile Leu Leu Glu Thr Asn Pro His Ala Val

195 200 205

Val Glu Ile Glu Ile Ser Glu Asp Met Gln Leu Val His Leu Asp Phe

210 215 220

Asn Ser Thr Pro Phe Ser Val His Asp Asp Ala Tyr Ile Leu Lys Leu

225 230 235 240

Met Gln Glu Gln Lys Gln Glu Gln Asn Arg Val Ser Ser Ser Ser Ser

245 250 255

Thr His His Gln Ser Gln His Ser Ser Ala His Ser Ser Ser Ser Ser

260 265 270

Cys Ile Ala Ser Gly Thr Ser Gly Pro Val Cys Trp Asn Ser Gly Ser

275 280 285

Ile Asp Thr Arg

290

<210>53

<211>1527

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(1527)

<400>53

atg aaa ggt tca att ctt act gtt ttg tca atg gag aat cat cat ccg 48

Met Lys Gly Ser Ile Leu Thr Val Leu Ser Met Glu Asn His His Pro

1 5 10 15

tca acg ctt tta tct atg gat tct agt ggc tca tct cat gaa gag ctt 96

Ser Thr Leu Leu Ser Met Asp Ser Ser Gly Ser Ser His Glu Glu Leu

20 25 30

gat ttg gag atg aac aat ggt aat agg caa atc act ctt tat aat cca 144

Asp Leu Glu Met Asn Asn Gly Asn Arg Gln Ile Thr Leu Tyr Asn Pro

35 40 45

cca gac att aat ctg cct ttg tct gta gga aga agc tct cct tct tgg 192

Pro Asp Ile Asn Leu Pro Leu Ser Val Gly Arg Ser Ser Pro Ser Trp

50 55 60

aat ttg gat tct tgt gat aac att ttg gat gtt ggt ctt agc tct cat 240

Asn Leu Asp Ser Cys Asp Asn Ile Leu Asp Val Gly Leu Ser Ser His

65 70 75 80

gtc tat gag acc gag acg ttt ctc aat gtg gtc ccg agt aaa gta gct 288

Val Tyr Glu Thr Glu Thr Phe Leu Asn Val Val Pro Ser Lys Val Ala

85 90 95

aag aag tgt ttg aaa cga ggg gat agt atg tgg gga gct tgg ttt ttc 336

Lys Lys Cys Leu Lys Arg Gly Asp Ser Met Trp Gly Ala Trp Phe Phe

100 105 110

ttt agc ttc tac ttc aga ccg gcg ttg aat gag aaa tcc aag tct aag 384

Phe Ser Phe Tyr Phe Arg Pro Ala Leu Asn Glu Lys Ser Lys Ser Lys

115 120 125

gtc att agg gaa agt ggt ggt ggt gga gga gga gga gga gga tgt ttt 432

Val Ile Arg Glu Ser Gly Gly Gly Gly Gly Gly Gly Gly Gly Cys Phe

130 135 140

act ggg ttt gat aaa tct gat ctc aag ctc gat gtt ttt ctt gtt cag 480

Thr Gly Phe Asp Lys Ser Asp Leu Lys Leu Asp Val Phe Leu Val Gln

145 150 155 160

cat gat atg gag aac atg tat atg tgg gct ttt aag gat aaa cct gag 528

His Asp Met Glu Asn Met Tyr Met Trp Ala Phe Lys Asp Lys Pro Glu

165 170 175

aat gcg ctt ggg aaa atg cag ttg aga agc tat atg aat ggg cat tct 576

Asn Ala Leu Gly Lys Met Gln Leu Arg Ser Tyr Met Asn Gly His Ser

180 185 190

cgt caa ggt gag cgt ccg ttt ccg ttt agt gcg gag aaa ggg ttt gtt 624

Arg Gln Gly Glu Arg Pro Phe Pro Phe Ser Ala Glu Lys Gly Phe Val

195 200 205

cgg tct cac aga atg cag agg aag cat tac agg gga ctc tct aat cct 672

Arg Ser His Arg Met Gln Arg Lys His Tyr Arg Gly Leu Ser Asn Pro

210 215 220

cag tgt ctt cac ggg att gag ttt gtg gct tcg ccg agt ttg ttt ggt 720

Gln Cys Leu His Gly Ile Glu Phe Val Ala Ser Pro Ser Leu Phe Gly

225 230 235 240

gtc ggt gaa gaa gat aag aag aga tgg atg gag ctc acg ggt cga gat 768

Val Gly Glu Glu Asp Lys Lys Arg Trp Met Glu Leu Thr Gly Arg Asp

245 250 255

ttg aag ttc act atc cct cct gat gct agt gat ttc ggt tca tgg aga 816

Leu Lys Phe Thr Ile Pro Pro Asp Ala Ser Asp Phe Gly Ser Trp Arg

260 265 270

aat ctt ccc aac aca gac atc gag cta gag aga cca gct cat gtt act 864

Asn Leu Pro Asn Thr Asp Ile Glu Leu Glu Arg Pro Ala His Val Thr

275 280 285

aaa gca gca ccg aat aac gcc aag aag att ctc aat ggc tcc ggc tta 912

Lys Ala Ala Pro Asn Asn Ala Lys Lys Ile Leu Asn Gly Ser Gly Leu

290 295 300

cat ttg aca agc aat gcg tct ttc agt agc aat ggg gac tcg tct gat 960

His Leu Thr Ser Asn Ala Ser Phe Ser Ser Asn Gly Asp Ser Ser Asp

305 310 315 320

caa tct cca gga gga gga gtc atc aac aac aag aag aga aaa gag ttt 1008

Gln Ser Pro Gly Gly Gly Val Ile Asn Asn Lys Lys Arg Lys Glu Phe

325 330 335

cta tct cct gga agc agc gaa gaa gaa tgc tgt ttg act gtt aac aac 1056

Leu Ser Pro Gly Ser Ser Glu Glu Glu Cys Cys Leu Thr Val Asn Asn

340 345 350

atc gag acc cac cac gcc aag gac ccg ccc agt tgg gta aac gac ttc 1104

Ile Glu Thr His His Ala Lys Asp Pro Pro Ser Trp Val Asn Asp Phe

355 360 365

acg gga gtg atg aag aat agc tgc gga cct gta act gct gca aaa acc 1152

Thr Gly Val Met Lys Asn Ser Cys Gly Pro Val Thr Ala Ala Lys Thr

370 375 380

gtc tat gag gac gaa gaa gct tat ctg gtc gta ata act cta cca ttt 1200

Val Tyr Glu Asp Glu Glu Ala Tyr Leu Val Val Ile Thr Leu Pro Phe

385 390 395 400

gtg gat ttg aac acc gtg aag gtt tca tgg agg aac aat atc aca aat 1248

Val Asp Leu Asn Thr Val Lys Val Ser Trp Arg Asn Asn Ile Thr Asn

405 410 415

gga atc gtg aag gtc acg gga cta agc act tcg agg gct tcg ttt gtg 1296

Gly Ile Val Lys Val Thr Gly Leu Ser Thr Ser Arg Ala Ser Phe Val

420 425 430

aag aga cgg gac cgg act ttc aag ctg gtt gat cag atg gct gag cat 1344

Lys Arg Arg Asp Arg Thr Phe Lys Leu Val Asp Gln Met Ala Glu His

435 440 445

tgt cct cca ggg gaa ttc atg agg gag ata caa ttg ccg aat cgg att 1392

Cys Pro Pro Gly Glu Phe Met Arg Glu Ile Gln Leu Pro Asn Arg Ile

450 455 460

ccg gaa gaa gca aat att gaa gca tac ttt gat ggg act gga cca gtt 1440

Pro Glu Glu Ala Asn Ile Glu Ala Tyr Phe Asp Gly Thr Gly Pro Val

465 470 475 480

tta gag att gtg gtt cca aaa ttg aga gga gga gtg gag gaa gaa cac 1488

Leu Glu Ile Val Val Pro Lys Leu Arg Gly Gly Val Glu Glu Glu His

485 490 495

gag gtt aga gtt tgt cta cgg tca cac cac ctc gga tga 1527

Glu Val Arg Val Cys Leu Arg Ser His His Leu Gly

500 505

<210>54

<211>508

<212>PRT

＜213〉Arabidopis thaliana

<400>54

Met Lys Gly Ser Ile Leu Thr Val Leu Ser Met Glu Asn His His Pro

1 5 10 15

Ser Thr Leu Leu Ser Met Asp Ser Ser Gly Ser Ser His Glu Glu Leu

20 25 30

Asp Leu Glu Met Asn Asn Gly Asn Arg Gln Ile Thr Leu Tyr Asn Pro

35 40 45

Pro Asp Ile Asn Leu Pro Leu Ser Val Gly Arg Ser Ser Pro Ser Trp

50 55 60

Asn Leu Asp Ser Cys Asp Asn Ile Leu Asp Val Gly Leu Ser Ser His

65 70 75 80

Val Tyr Glu Thr Glu Thr Phe Leu Asn Val Val Pro Ser Lys Val Ala

85 90 95

Lys Lys Cys Leu Lys Arg Gly Asp Ser Met Trp Gly Ala Trp Phe Phe

100 105 110

Phe Ser Phe Tyr Phe Arg Pro Ala Leu Asn Glu Lys Ser Lys Ser Lys

115 120 125

Val Ile Arg Glu Ser Gly Gly Gly Gly Gly Gly Gly Gly Gly Cys Phe

130 135 140

Thr Gly Phe Asp Lys Ser Asp Leu Lys Leu Asp Val Phe Leu Val Gln

145 150 155 160

His Asp Met Glu Asn Met Tyr Met Trp Ala Phe Lys Asp Lys Pro Glu

165 170 175

Asn Ala Leu Gly Lys Met Gln Leu Arg Ser Tyr Met Asn Gly His Ser

180 185 190

Arg Gln Gly Glu Arg Pro Phe Pro Phe Ser Ala Glu Lys Gly Phe Val

195 200 205

Arg Ser His Arg Met Gln Arg Lys His Tyr Arg Gly Leu Ser Asn Pro

210 215 220

Gln Cys Leu His Gly Ile Glu Phe Val Ala Ser Pro Ser Leu Phe Gly

225 230 235 240

Val Gly Glu Glu Asp Lys Lys Arg Trp Met Glu Leu Thr Gly Arg Asp

245 250 255

Leu Lys Phe Thr Ile Pro Pro Asp Ala Ser Asp Phe Gly Ser Trp Arg

260 265 270

Asn Leu Pro Asn Thr Asp Ile Glu Leu Glu Arg Pro Ala His Val Thr

275 280 285

Lys Ala Ala Pro Asn Asn Ala Lys Lys Ile Leu Asn Gly Ser Gly Leu

290 295 300

His Leu Thr Ser Asn Ala Ser Phe Ser Ser Asn Gly Asp Ser Ser Asp

305 310 315 320

Gln Ser Pro Gly Gly Gly Val Ile Asn Asn Lys Lys Arg Lys Glu Phe

325 330 335

Leu Ser Pro Gly Ser Ser Glu Glu Glu Cys Cys Leu Thr Val Asn Asn

340 345 350

Ile Glu Thr His His Ala Lys Asp Pro Pro Ser Trp Val Asn Asp Phe

355 360 365

Thr Gly Val Met Lys Asn Ser Cys Gly Pro Val Thr Ala Ala Lys Thr

370 375 380

Val Tyr Glu Asp Glu Glu Ala Tyr Leu Val Val Ile Thr Leu Pro Phe

385 390 395 400

Val Asp Leu Asn Thr Val Lys Val Ser Trp Arg Asn Asn Ile Thr Asn

405 410 415

Gly Ile Val Lys Val Thr Gly Leu Ser Thr Ser Arg Ala Ser Phe Val

420 425 430

Lys Arg Arg Asp Arg Thr Phe Lys Leu Val Asp Gln Met Ala Glu His

435 440 445

Cys Pro Pro Gly Glu Phe Met Arg Glu Ile Gln Leu Pro Asn Arg Ile

450 455 460

Pro Glu Glu Ala Asn Ile Glu Ala Tyr Phe Asp Gly Thr Gly Pro Val

465 470 475 480

Leu Glu Ile Val Val Pro Lys Leu Arg Gly Gly Val Glu Glu Glu His

485 490 495

Glu Val Arg Val Cys Leu Arg Ser His His Leu Gly

500 505

<210>55

<211>747

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(747)

<400>55

atg cat ttc tct tcg tct tca aca tcg tcc act tgg aca atc tta atc 48

Met His Phe Ser Ser Ser Ser Thr Ser Ser Thr Trp Thr Ile Leu Ile

1 5 10 15

aca ttg gga tgt ctt atg ctt cat gca tct ttg tcc gct gct caa ctc 96

Thr Leu Gly Cys Leu Met Leu His Ala Ser Leu Ser Ala Ala Gln Leu

20 25 30

acc cct acc ttc tac gat agg tca tgt cct aat gtc act aac atc gta 144

Thr Pro Thr Phe Tyr Asp Arg Ser Cys Pro Asn Val Thr Asn Ile Val

35 40 45

cga gaa acc att gta aat gag tta agg tcg gac cct cgt atc gct gcg 192

Arg Glu Thr Ile Val Asn Glu Leu Arg Ser Asp Pro Arg Ile Ala Ala

50 55 60

agc atc ctt cgt ctt cac ttc cac gac tgc ttt gtt aat ggt tgt gac 240

Ser Ile Leu Arg Leu His Phe His Asp Cys Phe Val Asn Gly Cys Asp

65 70 75 80

gca tcc atc ttg tta gac aac acg aca tca ttt cga aca gag aaa gat 288

Ala Ser Ile Leu Leu Asp Asn Thr Thr Ser Phe Arg Thr Glu Lys Asp

85 90 95

gcg ttt gga aac gca aat tcg gct cgg gga ttt cca gtg att gat aga 336

Ala Phe Gly Asn Ala Asn Ser Ala Arg Gly Phe Pro Val Ile Asp Arg

100 105 110

atg aaa gct gcg gtg gag agg gca tgc cca aga acc gtt tca tgc gca 384

Met Lys Ala Ala Val Glu Arg Ala Cys Pro Arg Thr Val Ser Cys Ala

115 120 125

gat atg ctc acc att gca gct caa caa tct gtc act ttg gca gga ggt 432

Asp Met Leu Thr Ile Ala Ala Gln Gln Ser Val Thr Leu Ala Gly Gly

130 135 140

cct tct tgg agg gtt cct ttg gga agg aga gac agt tta caa gca ttc 480

Pro Ser Trp Arg Val Pro Leu Gly Arg Arg Asp Ser Leu Gln Ala Phe

145 150 155 160

ctg gaa ctc gct aat gca aat ctt cca gct cca ttc ttt aca ctt cac 528

Leu Glu Leu Ala Asn Ala Asn Leu Pro Ala Pro Phe Phe Thr Leu His

165 170 175

aac tta aag cca gct tca gaa atg ttg gtc tcg atc gtc ctt ctg atc 576

Asn Leu Lys Pro Ala Ser Glu Met Leu Val Ser Ile Val Leu Leu Ile

180 185 190

tcg ttg ctc tct ccg gtg gtc aca cat ttg gta aaa atc aat gtc agt 624

Ser Leu Leu Ser Pro Val Val Thr His Leu Val Lys Ile Asn Val Ser

195 200 205

tta ttc ttg aca gat tat aca att tca gca aca cag gtt tac ccg acc 672

Leu Phe Leu Thr Asp Tyr Thr Ile Ser Ala Thr Gln Val Tyr Pro Thr

210 215 220

cta cac tca aca cta ctt acc tcc aaa ctc ttc gtg gac tat gcc ccc 720

Leu His Ser Thr Leu Leu Thr Ser Lys Leu Phe Val Asp Tyr Ala Pro

225 230 235 240

tta atg gca atc gaa gtg cct tgg tag 747

Leu Met Ala Ile Glu Val Pro Trp

245

<210>56

<211>248

<212>PRT

＜213〉Arabidopis thaliana

<400>56

Met His Phe Ser Ser Ser Ser Thr Ser Ser Thr Trp Thr Ile Leu Ile

1 5 10 15

Thr Leu Gly Cys Leu Met Leu His Ala Ser Leu Ser Ala Ala Gln Leu

20 25 30

Thr Pro Thr Phe Tyr Asp Arg Ser Cys Pro Asn Val Thr Asn Ile Val

35 40 45

Arg Glu Thr Ile Val Asn Glu Leu Arg Ser Asp Pro Arg Ile Ala Ala

50 55 60

Ser Ile Leu Arg Leu His Phe His Asp Cys Phe Val Asn Gly Cys Asp

65 70 75 80

Ala Ser Ile Leu Leu Asp Asn Thr Thr Ser Phe Arg Thr Glu Lys Asp

85 90 95

Ala Phe Gly Asn Ala Asn Ser Ala Arg Gly Phe Pro Val Ile Asp Arg

100 105 110

Met Lys Ala Ala Val Glu Arg Ala Cys Pro Arg Thr Val Ser Cys Ala

115 120 125

Asp Met Leu Thr Ile Ala Ala Gln Gln Ser Val Thr Leu Ala Gly Gly

130 135 140

Pro Ser Trp Arg Val Pro Leu Gly Arg Arg Asp Ser Leu Gln Ala Phe

145 150 155 160

Leu Glu Leu Ala Asn Ala Asn Leu Pro Ala Pro Phe Phe Thr Leu His

165 170 175

Asn Leu Lys Pro Ala Ser Glu Met Leu Val Ser Ile Val Leu Leu Ile

180 185 190

Ser Leu Leu Ser Pro Val Val Thr His Leu Val Lys Ile Asn Val Ser

195 200 205

Leu Phe Leu Thr Asp Tyr Thr Ile Ser Ala Thr Gln Val Tyr Pro Thr

210 215 220

Leu His Ser Thr Leu Leu Thr Ser Lys Leu Phe Val Asp Tyr Ala Pro

225 230 235 240

Leu Met Ala Ile Glu Val Pro Trp

245

<210>57

<211>1065

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(1065)

<400>57

atg caa ttc tct tca tct tct att act tct ttc act tgg aca gtt tta 48

Met Gln Phe Ser Ser Ser Ser Ile Thr Ser Phe Thr Trp Thr Val Leu

1 5 10 15

atc aca gtg gga tgt ctt atg ctt tgt gcg tct ttc tcc gat gct caa 96

Ile Thr Val Gly Cys Leu Met Leu Cys Ala Ser Phe Ser Asp Ala Gln

20 25 30

ctt acc cct act ttt tac gac act tca tgt cct acc gtc acc aac att 144

Leu Thr Pro Thr Phe Tyr Asp Thr Ser Cys Pro Thr Val Thr Asn Ile

35 40 45

gta aga gat acc att gtc aac gag cta aga tcg gac cct cgt atc gcc 192

Val Arg Asp Thr Ile Val Asn Glu Leu Arg Ser Asp Pro Arg Ile Ala

50 55 60

ggg agc atc ctt cgt ctt cac ttc cat gac tgc ttt gtt aat ggt tgt 240

Gly Ser Ile Leu Arg Leu His Phe His Asp Cys Phe Val Asn Gly Cys

65 70 75 80

gat gct tcg atc ttg tta gac aac acg aca tca ttt cga aca gag aaa 288

Asp Ala Ser Ile Leu Leu Asp Asn Thr Thr Ser Phe Arg Thr Glu Lys

85 90 95

gat gca ctt gga aat gca aat tca gcc cga gga ttt cca gtg att gat 336

Asp Ala Leu Gly Asn Ala Asn Ser Ala Arg Gly Phe Pro Val Ile Asp

100 105 110

aga atg aaa gct gcg gtg gag agg gca tgc cca aga acc gtt tca tgc 384

Arg Met Lys Ala Ala Val Glu Arg Ala Cys Pro Arg Thr Val Ser Cys

115 120 125

gca gat atg ctc acc att gct gct caa caa tct gtc act ttg gca gga 432

Ala Asp Met Leu Thr Ile Ala Ala Gln Gln Ser Val Thr Leu Ala Gly

130 135 140

ggt cct tct tgg aag gtt cct tta ggg aga aga gac agc tta caa gca 480

Gly Pro Ser Trp Lys Val Pro Leu Gly Arg Arg Asp Ser Leu Gln Ala

145 150 155 160

ttt cta gat ctt gct aac gca aat ctt cca gct cca ttc ttc aca ctt 528

Phe Leu Asp Leu Ala Asn Ala Asn Leu Pro Ala Pro Phe Phe Thr Leu

165 170 175

cca cag ctt aaa gcc aac ttc aaa aat gtt ggc ctc gat cgt cct tct 576

Pro Gln Leu Lys Ala Asn Phe Lys Asn Val Gly Leu Asp Arg Pro Ser

180 185 190

gat ctt gtt gcg ctc tcc ggg gct cac aca ttt ggt aaa aat caa tgt 624

Asp Leu Val Ala Leu Ser Gly Ala His Thr Phe Gly Lys Asn Gln Cys

195 200 205

cga ttc att atg gac aga tta tac aac ttt agc aac act gga tta cct 672

Arg Phe Ile Met Asp Arg Leu Tyr Asn Phe Ser Asn Thr Gly Leu Pro

210 215 220

gac cct aca ctc aac act act tac ctc caa act ctt cgt ggt caa tgt 720

Asp Pro Thr Leu Asn Thr Thr Tyr Leu Gln Thr Leu Arg Gly Gln Cys

225 230 235 240

cct cgc aat ggt aat caa agc gtc tta gtg gat ttc gat ctg cgt acg 768

Pro Arg Asn Gly Asn Gln Ser Val Leu Val Asp Phe Asp Leu Arg Thr

245 250 255

cct ttg gtt ttc gac aac aaa tac tat gtg aat ctt aaa gag caa aaa 816

Pro Leu Val Phe Asp Asn Lys Tyr Tyr Val Asn Leu Lys Glu Gln Lys

260 265 270

ggt ctt atc cag agc gac caa gag ttg ttc tct agc ccc aat gcc act 864

Gly Leu Ile Gln Ser Asp Gln Glu Leu Phe Ser Ser Pro Asn Ala Thr

275 280 285

gac aca atc ccc ttg gtg aga gca tat gct gat ggc aca caa aca ttc 912

Asp Thr Ile Pro Leu Val Arg Ala Tyr Ala Asp Gly Thr Gln Thr Phe

290 295 300

ttc aat gca ttc gtg gag gca atg aat agg atg gga aat att aca cca 960

Phe Asn Ala Phe Val Glu Ala Met Asn Arg Met Gly Asn Ile Thr Pro

305 310 315 320

act aca gga act caa gga caa atc agg ttg aat tgt aga gtg gtg aac 1008

Thr Thr Gly Thr Gln Gly Gln Ile Arg Leu Asn Cys Arg Val Val Asn

325 330 335

tcc aac tct cta ctc cat gat gtg gtg gat atc gtt gac ttt gta agt 1056

Ser Asn Ser Leu Leu His Asp Val Val Asp Ile Val Asp Phe Val Ser

340 345 350

tct atg tga 1065

Ser Met

<210>58

<211>354

<212>PRT

＜213〉Arabidopis thaliana

<400>58

Met Gln Phe Ser Ser Ser Ser Ile Thr Ser Phe Thr Trp Thr Val Leu

1 5 10 15

Ile Thr Val Gly Cys Leu Met Leu Cys Ala Ser Phe Ser Asp Ala Gln

20 25 30

Leu Thr Pro Thr Phe Tyr Asp Thr Ser Cys Pro Thr Val Thr Asn Ile

35 40 45

Val Arg Asp Thr Ile Val Asn Glu Leu Arg Ser Asp Pro Arg Ile Ala

50 55 60

Gly Ser Ile Leu Arg Leu His Phe His Asp Cys Phe Val Asn Gly Cys

65 70 75 80

Asp Ala Ser Ile Leu Leu Asp Asn Thr Thr Ser Phe Arg Thr Glu Lys

85 90 95

Asp Ala Leu Gly Asn Ala Asn Ser Ala Arg Gly Phe Pro Val Ile Asp

100 105 110

Arg Met Lys Ala Ala Val Glu Arg Ala Cys Pro Arg Thr Val Ser Cys

115 120 125

Ala Asp Met Leu Thr Ile Ala Ala Gln Gln Ser Val Thr Leu Ala Gly

130 135 140

Gly Pro Ser Trp Lys Val Pro Leu Gly Arg Arg Asp Ser Leu Gln Ala

145 150 155 160

Phe Leu Asp Leu Ala Asn Ala Asn Leu Pro Ala Pro Phe Phe Thr Leu

165 170 175

Pro Gln Leu Lys Ala Asn Phe Lys Asn Val Gly Leu Asp Arg Pro Ser

180 185 190

Asp Leu Val Ala Leu Ser Gly Ala His Thr Phe Gly Lys Asn Gln Cys

195 200 205

Arg Phe Ile Met Asp Arg Leu Tyr Asn Phe Ser Asn Thr Gly Leu Pro

210 215 220

Asp Pro Thr Leu Asn Thr Thr Tyr Leu Gln Thr Leu Arg Gly Gln Cys

225 230 235 240

Pro Arg Asn Gly Asn Gln Ser Val Leu Val Asp Phe Asp Leu Arg Thr

245 250 255

Pro Leu Val Phe Asp Asn Lys Tyr Tyr Val Asn Leu Lys Glu Gln Lys

260 265 270

Gly Leu Ile Gln Ser Asp Gln Glu Leu Phe Ser Ser Pro Asn Ala Thr

275 280 285

Asp Thr Ile Pro Leu Val Arg Ala Tyr Ala Asp Gly Thr Gln Thr Phe

290 295 300

Phe Asn Ala Phe Val Glu Ala Met Asn Arg Met Gly Asn Ile Thr Pro

305 310 315 320

Thr Thr Gly Thr Gln Gly Gln Ile Arg Leu Asn Cys Arg Val Val Asn

325 330 335

Ser Asn Ser Leu Leu His Asp Val Val Asp Ile Val Asp Phe Val Ser

340 345 350

Ser Met

<210>59

<211>1899

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(1899)

<400>59

atg gcg gac gaa tct caa tac tca tcg gat act tac tcc aac aaa cgc 48

Met Ala Asp Glu Ser Gln Tyr Ser Ser Asp Thr Tyr Ser Asn Lys Arg

1 5 10 15

aaa tac gaa gaa cca acc gct cct cct cca tca act cgc aga cct acc 96

Lys Tyr Glu Glu Pro Thr Ala Pro Pro Pro Ser Thr Arg Arg Pro Thr

20 25 30

ggc ttc tct tct ggt ccg atc cca tct gct tca gtt gat ccc acc gca 144

Gly Phe Ser Ser Gly Pro Ile Pro Ser Ala Ser Val Asp Pro Thr Ala

35 40 45

cct acc ggt ctt cca cct tct tct tac aac agc gtt cct cct ccg atg 192

Pro Thr Gly Leu Pro Pro Ser Ser Tyr Asn Ser Val Pro Pro Pro Met

50 55 60

gat gaa atc cag att gct aaa caa aaa gca caa gaa atc gct gct cgt 240

Asp Glu Ile Gln Ile Ala Lys Gln Lys Ala Gln Glu Ile Ala Ala Arg

65 70 75 80

ctt ctt aat agc gct gat gct aaa cgt cct cgt gtt gac aat ggt gct 288

Leu Leu Asn Ser Ala Asp Ala Lys Arg Pro Arg Val Asp Asn Gly Ala

85 90 95

tct tat gat tat ggt gac aac aaa gga ttt agc tca tat ccc tct gag 336

Ser Tyr Asp Tyr Gly Asp Asn Lys Gly Phe Ser Ser Tyr Pro Ser Glu

100 105 110

ggt aag cag atg tca ggg acg gtt ccg tct tcg ata ccg gtt tcg tat 384

Gly Lys Gln Met Ser Gly Thr Val Pro Ser Ser Ile Pro Val Ser Tyr

115 120 125

ggt agc ttt caa gga act act aag aag att gat att ccg aat atg aga 432

Gly Ser Phe Gln Gly Thr Thr Lys Lys Ile Asp Ile Pro Asn Met Arg

130 135 140

gtt ggt gtt atc att ggt aaa ggt gga gag act att aag tat ctt cag 480

Val Gly Val Ile Ile Gly Lys Gly Gly Glu Thr Ile Lys Tyr Leu Gln

145 150 155 160

ctt cag tct gga gct aag att cag gtt act aga gat atg gat gca gac 528

Leu Gln Ser Gly Ala Lys Ile Gln Val Thr Arg Asp Met Asp Ala Asp

165 170 175

cct aat tgt gct act agg act gtt gac cta act ggt acc cct gat cag 576

Pro Asn Cys Ala Thr Arg Thr Val Asp Leu Thr Gly Thr Pro Asp Gln

180 185 190

atc tca aag gct gaa cag ttg atc act gac gtc ctt caa gag gct gag 624

Ile Ser Lys Ala Glu Gln Leu Ile Thr Asp Val Leu Gln Glu Ala Glu

195 200 205

gca ggc aat aca gct ggt tca ggt gga gga ggc ggc cgt agg atg ggt 672

Ala Gly Asn Thr Ala Gly Ser Gly Gly Gly Gly Gly Arg Arg Met Gly

210 215 220

gga caa gca ggg gct gat caa ttt gtt atg aaa att ccg aat aac aag 720

Gly Gln Ala Gly Ala Asp Gln Phe Val Met Lys Ile Pro Asn Asn Lys

225 230 235 240

gtt ggt ttg ata att ggt aaa gga ggt gaa aca atc aaa tct atg caa 768

Val Gly Leu Ile Ile Gly Lys Gly Gly Glu Thr Ile Lys Ser Met Gln

245 250 255

gct aag act gga gct aga att cag gtt att cct tta cat ttg ccc cct 816

Ala Lys Thr Gly Ala Arg Ile Gln Val Ile Pro Leu His Leu Pro Pro

260 265 270

gga gac cca acg cca gaa cgg act ttg cag att gat ggg ata acc gaa 864

Gly Asp Pro Thr Pro Glu Arg Thr Leu Gln Ile Asp Gly Ile Thr Glu

275 280 285

cag att gaa cat gct aaa caa tta gtt aat gaa atc atc agt ggc gag 912

Gln Ile Glu His Ala Lys Gln Leu Val Asn Glu Ile Ile Ser Gly Glu

290 295 300

aac cgt atg aga aac tca gca atg ggt gga ggc tat cca caa caa ggt 960

Asn Arg Met Arg Asn Ser Ala Met Gly Gly Gly Tyr Pro Gln Gln Gly

305 310 315 320

ggt tat caa gcc cgc cca ccc tca agc tgg gca cca cct ggt ggt ccg 1008

Gly Tyr Gln Ala Arg Pro Pro Ser Ser Trp Ala Pro Pro Gly Gly Pro

325 330 335

cca gca caa cct ggt tat ggt ggt tac atg caa cca gga gca tat cca 1056

Pro Ala Gln Pro Gly Tyr Gly Gly Tyr Met Gln Pro Gly Ala Tyr Pro

340 345 350

ggt cca cct cag tat ggt caa tca cct tac gga agt tac cct caa caa 1104

Gly Pro Pro Gln Tyr Gly Gln Ser Pro Tyr Gly Ser Tyr Pro Gln Gln

355 360 365

act tca gct ggt tac tat gat cag tcc tct gtg cca cca tcc cag cag 1152

Thr Ser Ala Gly Tyr Tyr Asp Gln Ser Ser Val Pro Pro Ser Gln Gln

370 375 380

agc gcg caa ggt gag tat gat tat tac ggt cag caa cag tct cag caa 1200

Ser Ala Gln Gly Glu Tyr Asp Tyr Tyr Gly Gln Gln Gln Ser Gln Gln

385 390 395 400

cca agc agt ggt ggt agc tca gcc cca cca aca gat acc aca ggg tac 1248

Pro Ser Ser Gly Gly Ser Ser Ala Pro Pro Thr Asp Thr Thr Gly Tyr

405 410 415

aat tac tac cag cat gct tct ggt tat ggc caa gct ggt cag gga tac 1296

Asn Tyr Tyr Gln His Ala Ser Gly Tyr Gly Gln Ala Gly Gln Gly Tyr

420 425 430

cag caa gat ggg tat gga gct tac aat gcc tcg cag caa tcg gga tat 1344

Gln Gln Asp Gly Tyr Gly Ala Tyr Asn Ala Ser Gln Gln Ser Gly Tyr

435 440 445

ggt caa gct gct ggg tat gat caa cag ggt ggt tac ggc agc acc act 1392

Gly Gln Ala Ala Gly Tyr Asp Gln Gln Gly Gly Tyr Gly Ser Thr Thr

450 455 460

aat cca agt caa gag gaa gat gca tct caa gcc gct cca cca tcg tca 1440

Asn Pro Ser Gln Glu Glu Asp Ala Ser Gln Ala Ala Pro Pro Ser Ser

465 470 475 480

gct cag tct gga cag gct ggg tat ggt aca act ggt caa cag ccg cct 1488

Ala Gln Ser Gly Gln Ala Gly Tyr Gly Thr Thr Gly Gln Gln Pro Pro

485 490 495

gct caa ggt agt act ggt cag gca ggg tat gga gct cct cca act tct 1536

Ala Gln Gly Ser Thr Gly Gln Ala Gly Tyr Gly Ala Pro Pro Thr Ser

500 505 510

cag gct ggt tac agc agc cag cca gca gca gct tac aat tct ggg tat 1584

Gln Ala Gly Tyr Ser Ser Gln Pro Ala Ala Ala Tyr Asn Ser Gly Tyr

515 520 525

gga gca cca cca cct gct tca aag cca ccg act tat ggc cag agc cag 1632

Gly Ala Pro Pro Pro Ala Ser Lys Pro Pro Thr Tyr Gly Gln Ser Gln

530 535 540

cag tct cca ggt gct cct ggg agc tat ggt agt cag tct ggg tat gcc 1680

Gln Ser Pro Gly Ala Pro Gly Ser Tyr Gly Ser Gln Ser Gly Tyr Ala

545 550 555 560

caa cca gca gct tca ggg tat gga caa cct cca gcg tat ggg tat ggt 1728

Gln Pro Ala Ala Ser Gly Tyr Gly Gln Pro Pro Ala Tyr Gly Tyr Gly

565 570 575

caa gcg cca cag gga tat ggg tct tat gga gga tac aca caa cct gct 1776

Gln Ala Pro Gln Gly Tyr Gly Ser Tyr Gly Gly Tyr Thr Gln Pro Ala

580 585 590

gct ggt gga ggt tac tct tca gac ggg tct gct gga gcc act gct ggt 1824

Ala Gly Gly Gly Tyr Ser Ser Asp Gly Ser Ala Gly Ala Thr Ala Gly

595 600 605

ggt ggt ggt ggt aca cca gct tca cag agt gct gct cca cct gct gga 1872

Gly Gly Gly Gly Thr Pro Ala Ser Gln Ser Ala Ala Pro Pro Ala Gly

610 615 620

ccg ccc aaa gca tcc ccg aaa agt tga 1899

Pro Pro Lys Ala Ser Pro Lys Ser

625 630

<210>60

<211>632

<212>PRT

＜213〉Arabidopis thaliana

<400>60

Met Ala Asp Glu Ser Gln Tyr Ser Ser Asp Thr Tyr Ser Asn Lys Arg

1 5 10 15

Lys Tyr Glu Glu Pro Thr Ala Pro Pro Pro Ser Thr Arg Arg Pro Thr

20 25 30

Gly Phe Ser Ser Gly Pro Ile Pro Ser Ala Ser Val Asp Pro Thr Ala

35 40 45

Pro Thr Gly Leu Pro Pro Ser Ser Tyr Asn Ser Val Pro Pro Pro Met

50 55 60

Asp Glu Ile Gln Ile Ala Lys Gln Lys Ala Gln Glu Ile Ala Ala Arg

65 70 75 80

Leu Leu Asn Ser Ala Asp Ala Lys Arg Pro Arg Val Asp Asn Gly Ala

85 90 95

Ser Tyr Asp Tyr Gly Asp Asn Lys Gly Phe Ser Ser Tyr Pro Ser Glu

100 105 110

Gly Lys Gln Met Ser Gly Thr Val Pro Ser Ser Ile Pro Val Ser Tyr

115 120 125

Gly Ser Phe Gln Gly Thr Thr Lys Lys Ile Asp Ile Pro Asn Met Arg

130 135 140

Val Gly Val Ile Ile Gly Lys Gly Gly Glu Thr Ile Lys Tyr Leu Gln

145 150 155 160

Leu Gln Ser Gly Ala Lys Ile Gln Val Thr Arg Asp Met Asp Ala Asp

165 170 175

Pro Asn Cys Ala Thr Arg Thr Val Asp Leu Thr Gly Thr Pro Asp Gln

180 185 190

Ile Ser Lys Ala Glu Gln Leu Ile Thr Asp Val Leu Gln Glu Ala Glu

195 200 205

Ala Gly Asn Thr Ala Gly Ser Gly Gly Gly Gly Gly Arg Arg Met Gly

210 215 220

Gly Gln Ala Gly Ala Asp Gln Phe Val Met Lys Ile Pro Asn Asn Lys

225 230 235 240

Val Gly Leu Ile Ile Gly Lys Gly Gly Glu Thr Ile Lys Ser Met Gln

245 250 255

Ala Lys Thr Gly Ala Arg Ile Gln Val Ile Pro Leu His Leu Pro Pro

260 265 270

Gly Asp Pro Thr Pro Glu Arg Thr Leu Gln Ile Asp Gly Ile Thr Glu

275 280 285

Gln Ile Glu His Ala Lys Gln Leu Val Asn Glu Ile Ile Ser Gly Glu

290 295 300

Asn Arg Met Arg Asn Ser Ala Met Gly Gly Gly Tyr Pro Gln Gln Gly

305 310 315 320

Gly Tyr Gln Ala Arg Pro Pro Ser Ser Trp Ala Pro Pro Gly Gly Pro

325 330 335

Pro Ala Gln Pro Gly Tyr Gly Gly Tyr Met Gln Pro Gly Ala Tyr Pro

340 345 350

Gly Pro Pro Gln Tyr Gly Gln Ser Pro Tyr Gly Ser Tyr Pro Gln Gln

355 360 365

Thr Ser Ala Gly Tyr Tyr Asp Gln Ser Ser Val Pro Pro Ser Gln Gln

370 375 380

Ser Ala Gln Gly Glu Tyr Asp Tyr Tyr Gly Gln Gln Gln Ser Gln Gln

385 390 395 400

Pro Ser Ser Gly Gly Ser Ser Ala Pro Pro Thr Asp Thr Thr Gly Tyr

405 410 415

Asn Tyr Tyr Gln His Ala Ser Gly Tyr Gly Gln Ala Gly Gln Gly Tyr

420 425 430

Gln Gln Asp Gly Tyr Gly Ala Tyr Asn Ala Ser Gln Gln Ser Gly Tyr

435 440 445

Gly Gln Ala Ala Gly Tyr Asp Gln Gln Gly Gly Tyr Gly Ser Thr Thr

450 455 460

Asn Pro Ser Gln Glu Glu Asp Ala Ser Gln Ala Ala Pro Pro Ser Ser

465 470 475 480

Ala Gln Ser Gly Gln Ala Gly Tyr Gly Thr Thr Gly Gln Gln Pro Pro

485 490 495

Ala Gln Gly Ser Thr Gly Gln Ala Gly Tyr Gly Ala Pro Pro Thr Ser

500 505 510

Gln Ala Gly Tyr Ser Ser Gln Pro Ala Ala Ala Tyr Asn Ser Gly Tyr

515 520 525

Gly Ala Pro Pro Pro Ala Ser Lys Pro Pro Thr Tyr Gly Gln Ser Gln

530 535 540

Gln Ser Pro Gly Ala Pro Gly Ser Tyr Gly Ser Gln Ser Gly Tyr Ala

545 550 555 560

Gln Pro Ala Ala Ser Gly Tyr Gly Gln Pro Pro Ala Tyr Gly Tyr Gly

565 570 575

Gln Ala Pro Gln Gly Tyr Gly Ser Tyr Gly Gly Tyr Thr Gln Pro Ala

580 585 590

Ala Gly Gly Gly Tyr Ser Ser Asp Gly Ser Ala Gly Ala Thr Ala Gly

595 600 605

Gly Gly Gly Gly Thr Pro Ala Ser Gln Ser Ala Ala Pro Pro Ala Gly

610 615 620

Pro Pro Lys Ala Ser Pro Lys Ser

625 630

<210>61

<211>465

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(465)

<400>61

atg gtg act ccg att ttc tac gag gtt gat cat tct gat gtt agg aaa 48

Met Val Thr Pro Ile Phe Tyr Glu Val Asp His Ser Asp Val Arg Lys

1 5 10 15

cag acc gga gaa ttt gga aag gtc ttt gaa gag aca tgc aag aac aaa 96

Gln Thr Gly Glu Phe Gly Lys Val Phe Glu Glu Thr Cys Lys Asn Lys

20 25 30

aca gat gat gag aaa caa agg tgt agg aaa gct cta gca gat gtg gca 144

Thr Asp Asp Glu Lys Gln Arg Cys Arg Lys Ala Leu Ala Asp Val Ala

35 40 45

aat atg gct gga gag gat tct cga aac tgg tgt aat gaa gca aac atg 192

Asn Met Ala Gly Glu Asp Ser Arg Asn Trp Cys Asn Glu Ala Asn Met

50 55 60

att gaa aca att tcc aac gat gtt ccg aat aag ctc ata aca cca tcg 240

Ile Glu Thr Ile Ser Asn Asp Val Pro Asn Lys Leu Ile Thr Pro Ser

65 70 75 80

agt gat tta ggt gat ttc gtt ggt gtt gaa gct cat tta gag aga ttg 288

Ser Asp Leu Gly Asp Phe Val Gly Val Glu Ala His Leu Glu Arg Leu

85 90 95

agt tca ttg ttg tgc ttg gaa tct gaa gaa gct aga atg gta ggg att 336

Ser Ser Leu Leu Cys Leu Glu Ser Glu Glu Ala Arg Met Val Gly Ile

100 105 110

ggt aag agt acc cta gga aga gct ctt ttc agt caa ctc tct agc caa 384

Gly Lys Ser Thr Leu Gly Arg Ala Leu Phe Ser Gln Leu Ser Ser Gln

115 120 125

ttc ccc ctt cgc gct ttc gta act tat aaa cca acc gag aag aac agg 432

Phe Pro Leu Arg Ala Phe Val Thr Tyr Lys Pro Thr Glu Lys Asn Arg

130 135 140

ttt tat cag aaa ttt tat gtc aaa agg aca taa 465

Phe Tyr Gln Lys Phe Tyr Val Lys Arg Thr

145 150

<210>62

<211>154

<212>PRT

＜213〉Arabidopis thaliana

<400>62

Met Val Thr Pro Ile Phe Tyr Glu Val Asp His Ser Asp Val Arg Lys

1 5 10 15

Gln Thr Gly Glu Phe Gly Lys Val Phe Glu Glu Thr Cys Lys Asn Lys

20 25 30

Thr Asp Asp Glu Lys Gln Arg Cys Arg Lys Ala Leu Ala Asp Val Ala

35 40 45

Asn Met Ala Gly Glu Asp Ser Arg Asn Trp Cys Asn Glu Ala Asn Met

50 55 60

Ile Glu Thr Ile Ser Asn Asp Val Pro Asn Lys Leu Ile Thr Pro Ser

65 70 75 80

Ser Asp Leu Gly Asp Phe Val Gly Val Glu Ala His Leu Glu Arg Leu

85 90 95

Ser Ser Leu Leu Cys Leu Glu Ser Glu Glu Ala Arg Met Val Gly Ile

100 105 110

Gly Lys Ser Thr Leu Gly Arg Ala Leu Phe Ser Gln Leu Ser Ser Gln

115 120 125

Phe Pro Leu Arg Ala Phe Val Thr Tyr Lys Pro Thr Glu Lys Asn Arg

130 135 140

Phe Tyr Gln Lys Phe Tyr Val Lys Arg Thr

145 150

<210>63

<211>3312

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(3312)

<400>63

atg gct agc cgg aga tac gac gtt ttc cca agc ttc agt ggg gta gat 48

Met Ala Ser Arg Arg Tyr Asp Val Phe Pro Ser Phe Ser Gly Val Asp

1 5 10 15

gtt cgc aaa acg ttc ctc agc cat cta atc gag gcg ctc gac cgc aga 96

Val Arg Lys Thr Phe Leu Ser His Leu Ile Glu Ala Leu Asp Arg Arg

20 25 30

tca atc aat aca ttc atg gat cac ggc atc gtg aga agc tgc ata atc 144

Ser Ile Asn Thr Phe Met Asp His Gly Ile Val Arg Ser Cys Ile Ile

35 40 45

gcc gat gag ctt ata acg gcc att aga gaa gcg agg atc tca ata gtt 192

Ala Asp Glu Leu Ile Thr Ala Ile Arg Glu Ala Arg Ile Ser Ile Val

50 55 60

atc ttc tct gag aac tat gct tct tcc acg tgg tgc ttg aat gaa ttg 240

Ile Phe Ser Glu Asn Tyr Ala Ser Ser Thr Trp Cys Leu Asn Glu Leu

65 70 75 80

gtg gag atc cac aag tgt cac aag gac aaa gac ttg gat caa atg gtg 288

Val Glu Ile His Lys Cys His Lys Asp Lys Asp Leu Asp Gln Met Val

85 90 95

att ccg gtt ttc tac ggc gtt gat cct tct cat gtt aga aaa cag atc 336

Ile Pro Val Phe Tyr Gly Val Asp Pro Ser His Val Arg Lys Gln Ile

100 105 110

ggt ggc ttt ggc gat gtc ttt aaa aag aca tgc gag gac aaa cca gag 384

Gly Gly Phe Gly Asp Val Phe Lys Lys Thr Cys Glu Asp Lys Pro Glu

115 120 125

gat cag aaa caa aga tgg gtt aaa gct ctc aca gat ata tca aat tta 432

Asp Gln Lys Gln Arg Trp Val Lys Ala Leu Thr Asp Ile Ser Asn Leu

130 135 140

gcc ggg gag gat ctt cgg aac ggg cct agt gaa gca gcc atg gtt gta 480

Ala Gly Glu Asp Leu Arg Asn Gly Pro Ser Glu Ala Ala Met Val Val

145 150 155 160

aag ata gct aat gat gtt tcg aat aaa ctt ttt cct ctg cca aag ggt 528

Lys Ile Ala Asn Asp Val Ser Asn Lys Leu Phe Pro Leu Pro Lys Gly

165 170 175

ttt ggt gac tta gtc gga att gag gat cat ata gag gca ata aaa tta 576

Phe Gly Asp Leu Val Gly Ile Glu Asp His Ile Glu Ala Ile Lys Leu

180 185 190

aaa ctg tgc ttg gaa tcc aag gaa gct aga ata atg gtc ggg att tgg 624

Lys Leu Cys Leu Glu Ser Lys Glu Ala Arg Ile Met Val Gly Ile Trp

195 200 205

gga cag tca ggg att ggt aag agt act ata gga aga gct ctt ttc agt 672

Gly Gln Ser Gly Ile Gly Lys Ser Thr Ile Gly Arg Ala Leu Phe Ser

210 215 220

caa ctc tct agc cag ttc cac cat cgc gct ttc ata act tat aaa agc 720

Gln Leu Ser Ser Gln Phe His His Arg Ala Phe Ile Thr Tyr Lys Ser

225 230 235 240

acc agt ggt agt gac gtc tct ggc atg aag ttg agt tgg gaa aaa gaa 768

Thr Ser Gly Ser Asp Val Ser Gly Met Lys Leu Ser Trp Glu Lys Glu

245 250 255

ctt ctc tcg gaa atc tta ggt caa aag gac ata aag ata gag cat ttt 816

Leu Leu Ser Glu Ile Leu Gly Gln Lys Asp Ile Lys Ile Glu His Phe

260 265 270

ggt gtg gtg gag caa agg ttg aag cac aag aaa gtt ctt atc ctt ctt 864

Gly Val Val Glu Gln Arg Leu Lys His Lys Lys Val Leu Ile Leu Leu

275 280 285

gat gat gtg gat aat cta gag ttt ctt agg acc ttg gtg gga aaa gct 912

Asp Asp Val Asp Asn Leu Glu Phe Leu Arg Thr Leu Val Gly Lys Ala

290 295 300

gaa tgg ttt gga tct gga agc aga ata att gtg atc act caa gat agg 960

Glu Trp Phe Gly Ser Gly Ser Arg Ile Ile Val Ile Thr Gln Asp Arg

305 310 315 320

caa ctt ctc aag gct cat gag att gac ctt ata tat gag gtg aag ctc 1008

Gln Leu Leu Lys Ala His Glu Ile Asp Leu Ile Tyr Glu Val Lys Leu

325 330 335

cca tct caa ggt ctt gct ctt aag atg ata tgc caa tat gct ttt ggg 1056

Pro Ser Gln Gly Leu Ala Leu Lys Met Ile Cys Gln Tyr Ala Phe Gly

340 345 350

aaa tac tct cca cct gat gat ttt aag gaa cta gca ttt gaa gtt gca 1104

Lys Tyr Ser Pro Pro Asp Asp Phe Lys Glu Leu Ala Phe Glu Val Ala

355 360 365

aag ctt gcc ggt aat ctt cct ttg ggt ctc agt gtc ctt ggt tcg tct 1152

Lys Leu Ala Gly Asn Leu Pro Leu Gly Leu Ser Val Leu Gly Ser Ser

370 375 380

tta aaa cga agg agc aaa gaa gag tgg atg gag atg ctg gct gag ctc 1200

Leu Lys Arg Arg Ser Lys Glu Glu Trp Met Glu Met Leu Ala Glu Leu

385 390 395 400

caa aat ggt ttg aac aga gat att atg aaa aca tta aga gtc agc tac 1248

Gln Asn Gly Leu Asn Arg Asp Ile Met Lys Thr Leu Arg Val Ser Tyr

405 410 415

gtt aga tta gat cca aaa gat caa gat ata ttc cat tac att gca tgg 1296

Val Arg Leu Asp Pro Lys Asp Gln Asp Ile Phe His Tyr Ile Ala Trp

420 425 430

tta ttc aat ggt tgg aaa gtc aaa tcc atc aaa gac ttc ctc gga gat 1344

Leu Phe Asn Gly Trp Lys Val Lys Ser Ile Lys Asp Phe Leu Gly Asp

435 440 445

ggt gtt aat gtt aac att agg ctc aaa acg ttg gat gat aag tcc ctc 1392

Gly Val Asn Val Asn Ile Arg Leu Lys Thr Leu Asp Asp Lys Ser Leu

450 455 460

ata cgt tta aca ccg aat gat act ata gag atg cac aat ttg ctt cag 1440

Ile Arg Leu Thr Pro Asn Asp Thr Ile Glu Met His Asn Leu Leu Gln

465 470 475 480

aag ttg gct aca gaa att gat cgt gaa gag tct aat ggt aat cct gga 1488

Lys Leu Ala Thr Glu Ile Asp Arg Glu Glu Ser Asn Gly Asn Pro Gly

485 490 495

aaa cgt cga ttt ctg gag aat gct gag gaa att cta gac gta ttt acc 1536

Lys Arg Arg Phe Leu Glu Asn Ala Glu Glu Ile Leu Asp Val Phe Thr

500 505 510

gat aat acc ggc act gaa aaa ttg ctc gga ata gat ttc agc acg tca 1584

Asp Asn Thr Gly Thr Glu Lys Leu Leu Gly Ile Asp Phe Ser Thr Ser

515 520 525

tca gat tca caa atc gat aag cca ttt att tca ata gat gaa aac tcg 1632

Ser Asp Ser Gln Ile Asp Lys Pro Phe Ile Ser Ile Asp Glu Asn Ser

530 535 540

ttc caa ggc atg ctt aat ctc caa ttt cta aat att cat gat cat tac 1680

Phe Gln Gly Met Leu Asn Leu Gln Phe Leu Asn Ile His Asp His Tyr

545 550 555 560

tgg tgg caa ccg aga gaa acc aga ttg cgt cta cct aac ggc ctc gtt 1728

Trp Trp Gln Pro Arg Glu Thr Arg Leu Arg Leu Pro Asn Gly Leu Val

565 570 575

tac ttg cca cgt aaa ctc aaa tgg cta cgg tgg gaa aat tgt cca ttg 1776

Tyr Leu Pro Arg Lys Leu Lys Trp Leu Arg Trp Glu Asn Cys Pro Leu

580 585 590

aag cgt ttg cct tct aat ttt aag gct gag tat ctg gtt gaa ctc aga 1824

Lys Arg Leu Pro Ser Asn Phe Lys Ala Glu Tyr Leu Val Glu Leu Arg

595 600 605

atg gag aat agt gcc ctt gag aag ctg tgg aat gga act cag cct ctt 1872

Met Glu Asn Ser Ala Leu Glu Lys Leu Trp Asn Gly Thr Gln Pro Leu

610 615 620

gga agt ctc aag aag atg aat ttg agg aat tcc aac aat ttg aaa gaa 1920

Gly Ser Leu Lys Lys Met Asn Leu Arg Asn Ser Asn Asn Leu Lys Glu

625 630 635 640

att cca gat ctt tct tta gcc aca aac ctc gag gaa tta gat ctt tgt 1968

Ile Pro Asp Leu Ser Leu Ala Thr Asn Leu Glu Glu Leu Asp Leu Cys

645 650 655

aac tgc gaa gtg cta gaa agt ttt cca agt cct ctc aac tcg gaa tct 2016

Asn Cys Glu Val Leu Glu Ser Phe Pro Ser Pro Leu Asn Ser Glu Ser

660 665 670

ctt aag ttc ctc aat ctc cta cta tgc ccc cgg ttg aga aat ttc cct 2064

Leu Lys Phe Leu Asn Leu Leu Leu Cys Pro Arg Leu Arg Asn Phe Pro

675 680 685

gag att ata atg caa agt ttc atc ttt aca gat gaa att gag atc gag 2112

Glu Ile Ile Met Gln Ser Phe Ile Phe Thr Asp Glu Ile Glu Ile Glu

690 695 700

gta gca gat tgt tta tgg aac aag aat ctc cct gga ctc gat tat ctc 2160

Val Ala Asp Cys Leu Trp Asn Lys Asn Leu Pro Gly Leu Asp Tyr Leu

705 710 715 720

gat tgc ctt agg aga tgt aat cca agt aaa ttt cgc cca gaa cat ctc 2208

Asp Cys Leu Arg Arg Cys Asn Pro Ser Lys Phe Arg Pro Glu His Leu

725 730 735

aaa aac ctc aca gtg aga ggc aac aac atg ctt gag aag cta tgg gaa 2256

Lys Asn Leu Thr Val Arg Gly Asn Asn Met Leu Glu Lys Leu Trp Glu

740 745 750

ggc gtc cag tcg ctt ggg aaa ctc aag agg gtg gat ctg tca gaa tgt 2304

Gly Val Gln Ser Leu Gly Lys Leu Lys Arg Val Asp Leu Ser Glu Cys

755 760 765

gaa aac atg ata gaa att cca gac ctt tca aag gcc acc aat ctg gag 2352

Glu Asn Met Ile Glu Ile Pro Asp Leu Ser Lys Ala Thr Asn Leu Glu

770 775 780

att ttg gat ctc tca aat tgc aaa agt ttg gtg atg tta cct tct aca 2400

Ile Leu Asp Leu Ser Asn Cys Lys Ser Leu Val Met Leu Pro Ser Thr

785 790 795 800

att ggg aat ctc caa aaa tta tac acg tta aat atg gaa gaa tgc aca 2448

Ile Gly Asn Leu Gln Lys Leu Tyr Thr Leu Asn Met Glu Glu Cys Thr

805 810 815

ggg ctg aag gtt ctt cct atg gat atc aac ttg tca tct ctc cat aca 2496

Gly Leu Lys Val Leu Pro Met Asp Ile Asn Leu Ser Ser Leu His Thr

820 825 830

gtc cat ctc aaa ggg tgc tca agt ttg aga ttt atc cct cag att tca 2544

Val His Leu Lys Gly Cys Ser Ser Leu Arg Phe Ile Pro Gln Ile Ser

835 840 845

aaa agt att gca gta ctc aat cta gat gac act gcc att gaa gaa gtt 2592

Lys Ser Ile Ala Val Leu Asn Leu Asp Asp Thr Ala Ile Glu Glu Val

850 855 860

cca tgt ttt gag aat ttc tcg agg ctc atg gaa tta tcg atg cgt ggt 2640

Pro Cys Phe Glu Asn Phe Ser Arg Leu Met Glu Leu Ser Met Arg Gly

865 870 875 880

tgc aag tcg ttg aga aga ttt cct cag att tca act agt att caa gaa 2688

Cys Lys Ser Leu Arg Arg Phe Pro Gln Ile Ser Thr Ser Ile Gln Glu

885 890 895

ctc aat cta gct gac acc gcc att gaa caa gtt ccc tgc ttc att gag 2736

Leu Asn Leu Ala Asp Thr Ala Ile Glu Gln Val Pro Cys Phe Ile Glu

900 905 910

aaa ttt tcg agg ctc aag gta cta aat atg agt ggt tgc aaa atg ttg 2784

Lys Phe Ser Arg Leu Lys Val Leu Asn Met Ser Gly Cys Lys Met Leu

915 920 925

aaa aac ata tcc ccg aac att ttc aga ctg aca agg ctt atg aag gtc 2832

Lys Asn Ile Ser Pro Asn Ile Phe Arg Leu Thr Arg Leu Met Lys Val

930 935 940

gac ttt aca gac tgt gga ggt gtc atc aca gcg ttg agt ctt cta tct 2880

Asp Phe Thr Asp Cys Gly Gly Val Ile Thr Ala Leu Ser Leu Leu Ser

945 950 955 960

aaa tta gac gtc aat gat gtg gaa ttt aag ttt aac ggg acg aga gta 2928

Lys Leu Asp Val Asn Asp Val Glu Phe Lys Phe Asn Gly Thr Arg Val

965 970 975

aaa aga tgc ggc ata cga ctc ttg aat gtg tct aca tct ccg gat gat 2976

Lys Arg Cys Gly Ile Arg Leu Leu Asn Val Ser Thr Ser Pro Asp Asp

980 985 990

agt gag gga agc tct gaa aca gaa tct ccg gat gat agt gat gga gac 3024

Ser Glu Gly Ser Ser Glu Thr Glu Ser Pro Asp Asp Ser Asp Gly Asp

995 1000 1005

tct gta aca gag tac cac caa cag tct gga gaa aaa tgt gat gat 3069

Ser Val Thr Glu Tyr His Gln Gln Ser Gly Glu Lys Cys Asp Asp

1010 1015 1020

gta gag act gaa agt agc aag aag cgg atg cgg atg aca tta gga 3114

Val Glu Thr Glu Ser Ser Lys Lys Arg Met Arg Met Thr Leu Gly

1025 1030 1035

aac tct gaa aaa tat ttc aac tta ccc tgt ggc caa ata gta aca 3159

Asn Ser Glu Lys Tyr Phe Asn Leu Pro Cys Gly Gln Ile Val Thr

1040 1045 1050

gac act gtt ccg tta ggg tgg gga gaa tca tca tca gtt tct ttt 3204

Asp Thr Val Pro Leu Gly Trp Gly Glu Ser Ser Ser Val Ser Phe

1055 1060 1065

aat cca tgg ctg gag ggg gaa gct ttg tgt gtt gat tcc atg att 3249

Asn Pro Trp Leu Glu Gly Glu Ala Leu Cys Val Asp Ser Met Ile

1070 1075 1080

act gaa caa caa gat gca caa att cat ata gct aat gtg gat tgg 3294

Thr Glu Gln Gln Asp Ala Gln Ile His Ile Ala Asn Val Asp Trp

1085 1090 1095

gag tgg gag tta tgg taa 3312

Glu Trp Glu Leu Trp

1100

<210>64

<211>1103

<212>PRT

＜213〉Arabidopis thaliana

<400>64

Met Ala Ser Arg Arg Tyr Asp Val Phe Pro Ser Phe Ser Gly Val Asp

1 5 10 15

Val Arg Lys Thr Phe Leu Ser His Leu Ile Glu Ala Leu Asp Arg Arg

20 25 30

Ser Ile Asn Thr Phe Met Asp His Gly Ile Val Arg Ser Cys Ile Ile

35 40 45

Ala Asp Glu Leu Ile Thr Ala Ile Arg Glu Ala Arg Ile Ser Ile Val

50 55 60

Ile Phe Ser Glu Asn Tyr Ala Ser Ser Thr Trp Cys Leu Asn Glu Leu

65 70 75 80

Val Glu Ile His Lys Cys His Lys Asp Lys Asp Leu Asp Gln Met Val

85 90 95

Ile Pro Val Phe Tyr Gly Val Asp Pro Ser His Val Arg Lys Gln Ile

100 105 110

Gly Gly Phe Gly Asp Val Phe Lys Lys Thr Cys Glu Asp Lys Pro Glu

115 120 125

Asp Gln Lys Gln Arg Trp Val Lys Ala Leu Thr Asp Ile Ser Asn Leu

130 135 140

Ala Gly Glu Asp Leu Arg Asn Gly Pro Ser Glu Ala Ala Met Val Val

145 150 155 160

Lys Ile Ala Asn Asp Val Ser Asn Lys Leu Phe Pro Leu Pro Lys Gly

165 170 175

Phe Gly Asp Leu Val Gly Ile Glu Asp His Ile Glu Ala Ile Lys Leu

180 185 190

Lys Leu Cys Leu Glu Ser Lys Glu Ala Arg Ile Met Val Gly Ile Trp

195 200 205

Gly Gln Ser Gly Ile Gly Lys Ser Thr Ile Gly Arg Ala Leu Phe Ser

210 215 220

Gln Leu Ser Ser Gln Phe His His Arg Ala Phe Ile Thr Tyr Lys Ser

225 230 235 240

Thr Ser Gly Ser Asp Val Ser Gly Met Lys Leu Ser Trp Glu Lys Glu

245 250 255

Leu Leu Ser Glu Ile Leu Gly Gln Lys Asp Ile Lys Ile Glu His Phe

260 265 270

Gly Val Val Glu Gln Arg Leu Lys His Lys Lys Val Leu Ile Leu Leu

275 280 285

Asp Asp Val Asp Asn Leu Glu Phe Leu Arg Thr Leu Val Gly Lys Ala

290 295 300

Glu Trp Phe Gly Ser Gly Ser Arg Ile Ile Val Ile Thr Gln Asp Arg

305 310 315 320

Gln Leu Leu Lys Ala His Glu Ile Asp Leu Ile Tyr Glu Val Lys Leu

325 330 335

Pro Ser Gln Gly Leu Ala Leu Lys Met Ile Cys Gln Tyr Ala Phe Gly

340 345 350

Lys Tyr Ser Pro Pro Asp Asp Phe Lys Glu Leu Ala Phe Glu Val Ala

355 360 365

Lys Leu Ala Gly Asn Leu Pro Leu Gly Leu Ser Val Leu Gly Ser Ser

370 375 380

Leu Lys Arg Arg Ser Lys Glu Glu Trp Met Glu Met Leu Ala Glu Leu

385 390 395 400

Gln Asn Gly Leu Asn Arg Asp Ile Met Lys Thr Leu Arg Val Ser Tyr

405 410 415

Val Arg Leu Asp Pro Lys Asp Gln Asp Ile Phe His Tyr Ile Ala Trp

420 425 430

Leu Phe Asn Gly Trp Lys Val Lys Ser Ile Lys Asp Phe Leu Gly Asp

435 440 445

Gly Val Asn Val Asn Ile Arg Leu Lys Thr Leu Asp Asp Lys Ser Leu

450 455 460

Ile Arg Leu Thr Pro Asn Asp Thr Ile Glu Met His Asn Leu Leu Gln

465 470 475 480

Lys Leu Ala Thr Glu Ile Asp Arg Glu Glu Ser Asn Gly Asn Pro Gly

485 490 495

Lys Arg Arg Phe Leu Glu Asn Ala Glu Glu Ile Leu Asp Val Phe Thr

500 505 510

Asp Asn Thr Gly Thr Glu Lys Leu Leu Gly Ile Asp Phe Ser Thr Ser

515 520 525

Ser Asp Ser Gln Ile Asp Lys Pro Phe Ile Ser Ile Asp Glu Asn Ser

530 535 540

Phe Gln Gly Met Leu Asn Leu Gln Phe Leu Asn Ile His Asp His Tyr

545 550 555 560

Trp Trp Gln Pro Arg Glu Thr Arg Leu Arg Leu Pro Asn Gly Leu Val

565 570 575

Tyr Leu Pro Arg Lys Leu Lys Trp Leu Arg Trp Glu Asn Cys Pro Leu

580 585 590

Lys Arg Leu Pro Ser Asn Phe Lys Ala Glu Tyr Leu Val Glu Leu Arg

595 600 605

Met Glu Asn Ser Ala Leu Glu Lys Leu Trp Asn Gly Thr Gln Pro Leu

610 615 620

Gly Ser Leu Lys Lys Met Asn Leu Arg Asn Ser Asn Asn Leu Lys Glu

625 630 635 640

Ile Pro Asp Leu Ser Leu Ala Thr Asn Leu Glu Glu Leu Asp Leu Cys

645 650 655

Asn Cys Glu Val Leu Glu Ser Phe Pro Ser Pro Leu Asn Ser Glu Ser

660 665 670

Leu Lys Phe Leu Asn Leu Leu Leu Cys Pro Arg Leu Arg Asn Phe Pro

675 680 685

Glu Ile Ile Met Gln Ser Phe Ile Phe Thr Asp Glu Ile Glu Ile Glu

690 695 700

Val Ala Asp Cys Leu Trp Asn Lys Asn Leu Pro Gly Leu Asp Tyr Leu

705 710 715 720

Asp Cys Leu Arg Arg Cys Asn Pro Ser Lys Phe Arg Pro Glu His Leu

725 730 735

Lys Asn Leu Thr Val Arg Gly Asn Asn Met Leu Glu Lys Leu Trp Glu

740 745 750

Gly Val Gln Ser Leu Gly Lys Leu Lys Arg Val Asp Leu Ser Glu Cys

755 760 765

Glu Asn Met Ile Glu Ile Pro Asp Leu Ser Lys Ala Thr Asn Leu Glu

770 775 780

Ile Leu Asp Leu Ser Asn Cys Lys Ser Leu Val Met Leu Pro Ser Thr

785 790 795 800

Ile Gly Asn Leu Gln Lys Leu Tyr Thr Leu Asn Met Glu Glu Cys Thr

805 810 815

Gly Leu Lys Val Leu Pro Met Asp Ile Asn Leu Ser Ser Leu His Thr

820 825 830

Val His Leu Lys Gly Cys Ser Ser Leu Arg Phe Ile Pro Gln Ile Ser

835 840 845

Lys Ser Ile Ala Val Leu Asn Leu Asp Asp Thr Ala Ile Glu Glu Val

850 855 860

Pro Cys Phe Glu Asn Phe Ser Arg Leu Met Glu Leu Ser Met Arg Gly

865 870 875 880

Cys Lys Ser Leu Arg Arg Phe Pro Gln Ile Ser Thr Ser Ile Gln Glu

885 890 895

Leu Asn Leu Ala Asp Thr Ala Ile Glu Gln Val Pro Cys Phe Ile Glu

900 905 910

Lys Phe Ser Arg Leu Lys Val Leu Asn Met Ser Gly Cys Lys Met Leu

915 920 925

Lys Asn Ile Ser Pro Asn Ile Phe Arg Leu Thr Arg Leu Met Lys Val

930 935 940

Asp Phe Thr Asp Cys Gly Gly Val Ile Thr Ala Leu Ser Leu Leu Ser

945 950 955 960

Lys Leu Asp Val Asn Asp Val Glu Phe Lys Phe Asn Gly Thr Arg Val

965 970 975

Lys Arg Cys Gly Ile Arg Leu Leu Asn Val Ser Thr Ser Pro Asp Asp

980 985 990

Ser Glu Gly Ser Ser Glu Thr Glu Ser Pro Asp Asp Ser Asp Gly Asp

995 1000 1005

Ser Val Thr Glu Tyr His Gln Gln Ser Gly Glu Lys Cys Asp Asp

1010 1015 1020

Val Glu Thr Glu Ser Ser Lys Lys Arg Met Arg Met Thr Leu Gly

1025 1030 1035

Asn Ser Glu Lys Tyr Phe Asn Leu Pro Cys Gly Gln Ile Val Thr

1040 1045 1050

Asp Thr Val Pro Leu Gly Trp Gly Glu Ser Ser Ser Val Ser Phe

1055 1060 1065

Asn Pro Trp Leu Glu Gly Glu Ala Leu Cys Val Asp Ser Met Ile

1070 1075 1080

Thr Glu Gln Gln Asp Ala Gln Ile His Ile Ala Asn Val Asp Trp

1085 1090 1095

Glu Trp Glu Leu Trp

1100

<210>65

<211>1341

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1).. (1341)

<400>65

atg gcg aac ttg gtc tta tca gaa tgt ggt ata cga cct ctc ccc aga 48

Met Ala Asn Leu Val Leu Ser Glu Cys Gly Ile Arg Pro Leu Pro Arg

1 5 10 15

atc tac aca aca ccc aga tcc aat ttc ctc tcc aac aac aac aaa ttc 96

Ile Tyr Thr Thr Pro Arg Ser Asn Phe Leu Ser Asn Asn Asn Lys Phe

20 25 30

aga cca tca ctt tct tct tct tct tac aaa aca tca tca tct cct ctg 144

Arg Pro Ser Leu Ser Ser Ser Ser Tyr Lys Thr Ser Ser Ser Pro Leu

35 40 45

tct ttt ggt ctg aat tca cga gat ggg ttc acg agg aat tgg gcg ttg 192

Ser Phe Gly Leu Asn Ser Arg Asp Gly Phe Thr Arg Asn Trp Ala Leu

50 55 60

aat gtg agc aca cca tta acg aca cca ata ttt gag gag tct cca ttg 240

Asn Val Ser Thr Pro Leu Thr Thr Pro Ile Phe Glu Glu Ser Pro Leu

65 70 75 80

gag gaa gat aat aaa cag aga ttc gat cca ggt gcg cct cct ccg ttc 288

Glu Glu Asp Asn Lys Gln Arg Phe Asp Pro Gly Ala Pro Pro Pro Phe

85 90 95

aat tta gct gat att aga gca gct ata cct aag cat tgt tgg gtt aag 336

Asn Leu Ala Asp Ile Arg Ala Ala Ile Pro Lys His Cys Trp Val Lys

100 105 110

aat cca tgg aag tct ttg agt tat gtc gtc aga gac gtc gct atc gtc 384

Asn Pro Trp Lys Ser Leu Ser Tyr Val ValArg Asp Val Ala Ile Val

l15 120 125

ttt gca ttg gct gct gga gct gct tac ctc aac aat tgg att gtt tgg 432

Phe Ala Leu Ala Ala Gly Ala Ala Tyr Leu Asn Asn Trp Ile Val Trp

130 135 140

cct ctc tat tgg ctc gct caa gga acc atg ttt tgg gct ctc ttt gtt 480

Pro Leu Tyr Trp Leu Ala Gln Gly Thr Met Phe Trp Ala Leu Phe Val

145 150 155 160

ctt ggt cat gac tgt gga cat ggt agt ttc tca aat gat ccg aag ttg 528

Leu Gly His Asp Cys Gly His Gly Ser Phe Ser Asn Asp Pro Lys Leu

165 170 175

aac agt gtg gtc ggt cat ctt ctt cat tcc tca att ctg gtc cca tac 576

Asn Ser Val Val Gly His Leu Leu His Ser Ser Ile Leu Val Pro Tyr

180 185 190

cat ggc agg aga att agt cac aga act cac cac cag aac cat gga cat 624

His Gly Arg Arg Ile Ser His Arg Thr His His Gln Asn His Gly His

195 200 205

gtt gag aat gac gaa tct tgg cat cct atg tct gag aaa atc tac aat 672

Val Glu Asn Asp Glu Ser Trp His Pro Met Ser Glu Lys Ile Tyr Asn

210 215 220

act ttg gac aag ccg act aga ttc ttt aga ttt aca ctg cct ctc gtg 720

Thr Leu Asp Lys Pro Thr Arg Phe Phe Arg Phe Thr Leu Pro Leu Val

225 230 235 240

atg ctt gca tac cct ttc tac ttg tgg gct cga agt ccg ggg aaa aag 768

Met Leu Ala Tyr Pro Phe Tyr Leu Trp Ala Arg Ser Pro Gly Lys Lys

245 250 255

ggt tct cat tac cat cca gac agt gac ttg ttc ctc cct aaa gag aga 816

Gly Ser His Tyr His Pro Asp Ser Asp Leu Phe Leu Pro Lys Glu Arg

260 265 270

aag gat gtc ctc act tct act gct tgt tgg act gca atg gct gct ctg 864

Lys Asp Val Leu Thr Ser Thr Ala Cys Trp Thr Ala Met Ala Ala Leu

275 280 285

ctt gtt tgt ctc aac ttc aca atc ggt cca att caa atg ctc aaa ctt 912

Leu Val Cys Leu Asn Phe Thr Ile Gly Pro Ile Gln Met Leu Lys Leu

290 295 300

tat gga att cct tac tgg ata aat gta atg tgg ttg gac ttt gtg act 960

Tyr Gly Ile Pro Tyr Trp Ile Asn Val Met Trp Leu Asp Phe Val Thr

305 310 315 320

tac ctg cat cac cat ggt cat gaa gat aag ctt cct tgg tac cgt ggc 1008

Tyr Leu His His His Gly His Glu Asp Lys Leu Pro Trp Tyr Arg Gly

325 330 335

aag gag tgg agt tac ctg aga gga gga ctt aca aca ttg gat cgt gac 1056

Lys Glu Trp Ser Tyr Leu Arg Gly Gly Leu Thr Thr Leu Asp Arg Asp

340 345 350

tac gga ttg atc aat aac atc cat cat gat att gga act cat gtg ata 1104

Tyr Gly Leu Ile Asn Asn Ile His His Asp Ile Gly Thr His Val Ile

355 360 365

cat cat ctt ttc ccg cag atc cca cat tat cat cta gta gaa gca aca 1152

His His Leu Phe Pro Gln Ile Pro His Tyr His Leu Val Glu Ala Thr

370 375 380

gaa gca gct aaa cca gta tta ggg aag tat tac agg gag cct gat aag 1200

Glu Ala Ala Lys Pro Val Leu Gly Lys Tyr Tyr Arg Glu Pro Asp Lys

385 390 395 400

tct gga ccg ttg cca tta cat tta ctg gaa att cta gcg aaa agt ata 1248

Ser Gly Pro Leu Pro Leu His Leu Leu Glu Ile Leu Ala Lys Ser Ile

405 410 415

aaa gaa gat cat tac gtg agc gac gaa gga gaa gtt gta tac tat aaa 1296

Lys Glu Asp His Tyr Val Ser Asp Glu Gly Glu Val Val Tyr Tyr Lys

420 425 430

gca gat cca aat ctc tat gga gag gtc aaa gta aga gca gat tga 1341

Ala Asp Pro Asn Leu Tyr Gly Glu Val Lys Val Arg Ala Asp

435 440 445

<210>66

<211>446

<212>PRT

＜213〉Arabidopis thaliana

<400>66

Met Ala Asn Leu Val Leu Ser Glu Cys Gly Ile Arg Pro Leu Pro Arg

1 5 10 15

Ile Tyr Thr Thr Pro Arg Ser Asn Phe Leu Ser Asn Asn Asn Lys Phe

20 25 30

Arg Pro Ser Leu Ser Ser Ser Ser Tyr Lys Thr Ser Ser Ser Pro Leu

35 40 45

Ser Phe Gly Leu Asn Ser Arg Asp Gly Phe Thr Arg Asn Trp Ala Leu

50 55 60

Asn Val Ser Thr Pro Leu Thr Thr Pro Ile Phe Glu Glu Ser Pro Leu

65 70 75 80

Glu Glu Asp Asn Lys Gln Arg Phe Asp Pro Gly Ala Pro Pro Pro Phe

85 90 95

Asn Leu Ala Asp Ile Arg Ala Ala Ile Pro Lys His Cys Trp Val Lys

100 105 110

Asn Pro Trp Lys Ser Leu Ser Tyr Val Val Arg Asp Val Ala Ile Val

115 120 125

Phe Ala Leu Ala Ala Gly Ala Ala Tyr Leu Asn Asn Trp Ile Val Trp

130 135 140

Pro Leu Tyr Trp Leu Ala Gln Gly Thr Met Phe Trp Ala Leu Phe Val

145 150 155 160

Leu Gly His Asp Cys Gly His Gly Ser Phe Ser Asn Asp Pro Lys Leu

165 170 175

Asn Ser Val Val Gly His Leu Leu His Ser Ser Ile Leu Val Pro Tyr

180 185 190

His Gly Arg Arg Ile Ser His Arg Thr His His Gln Asn His Gly His

195 200 205

Val Glu Asn Asp Glu Ser Trp His Pro Met Ser Glu Lys Ile Tyr Asn

210 215 220

Thr Leu Asp Lys Pro Thr Arg Phe Phe Arg Phe Thr Leu Pro Leu Val

225 230 235 240

Met Leu Ala Tyr Pro Phe Tyr Leu Trp Ala Arg Ser Pro Gly Lys Lys

245 250 255

Gly Ser His Tyr His Pro Asp Ser Asp Leu Phe Leu Pro Lys Glu Arg

260 265 270

Lys Asp Val Leu Thr Ser Thr Ala Cys Trp Thr Ala Met Ala Ala Leu

275 280 285

Leu Val Cys Leu Asn Phe Thr Ile Gly Pro Ile Gln Met Leu Lys Leu

290 295 300

Tyr Gly Ile Pro Tyr Trp Ile Asn Val Met Trp Leu Asp Phe Val Thr

305 310 315 320

Tyr Leu His His His Gly His Glu Asp Lys Leu Pro Trp Tyr Arg Gly

325 330 335

Lys Glu Trp Ser Tyr Leu Arg Gly Gly Leu Thr Thr Leu Asp Arg Asp

340 345 350

Tyr Gly Leu Ile Asn Asn Ile His His Asp Ile Gly Thr His Val Ile

355 360 365

His His Leu Phe Pro Gln Ile Pro His Tyr His Leu Val Glu Ala Thr

370 375 380

Glu Ala Ala Lys Pro Val Leu Gly Lys Tyr Tyr Arg Glu Pro Asp Lys

385 390 395 400

Ser Gly Pro Leu Pro Leu His Leu Leu Glu Ile Leu Ala Lys Ser Ile

405 410 415

Lys Glu Asp His Tyr Val Ser Asp Glu Gly Glu Val Val Tyr Tyr Lys

420 425 430

Ala Asp Pro Asn Leu Tyr Gly Glu Val Lys Val Arg Ala Asp

435 440 445

<210>67

<211>1578

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(1578)

<400>67

atg gag gac gaa cca aag ctc cca acc gat gac ggt cca act ttc aac 48

Met Glu Asp Glu Pro Lys Leu Pro Thr Asp Asp Gly Pro Thr Phe Asn

1 5 10 15

gaa tcg tgt aaa atc tcg tct gag ata ttg acc gcc ggt gat cgg aaa 96

Glu Ser Cys Lys Ile Ser Ser Glu Ile Leu Thr Ala Gly Asp Arg Lys

20 25 30

tta ctt aaa gtt gaa ctc ctc aaa gag gag acc acg ctc gta tcg tgg 144

Leu Leu Lys Val Glu Leu Leu Lys Glu Glu Thr Thr Leu Val Ser Trp

35 40 45

aag aag ctt atg gat gag gct agc aaa gaa aac ggc ggc ttg ttc gtt 192

Lys Lys Leu Met Asp Glu Ala Ser Lys Glu Asn Gly Gly Leu Phe Val

50 55 60

tcg gct ccc gaa cgg ctt ctt aat gcc aac cct aac ctc gag ttt cgc 240

Ser Ala Pro Glu Arg Leu Leu Asn Ala Asn Pro Asn Leu Glu Phe Arg

65 70 75 80

ctt gca ccg ggg gca caa aca gag aat gaa atg gtg aat caa cct cat 288

Leu Ala Pro Gly Ala Gln Thr Glu Asn Glu Met Val Asn Gln Pro His

85 90 95

cct aat cgt ctt aac tct gtt ata gcc aag att gag aga ctt tat atg 336

Pro Asn Arg Leu Asn Ser Val Ile Ala Lys Ile Glu Arg Leu Tyr Met

100 105 110

ggt aaa gac ggt agt gat ggg gaa gag tta gac ggt gct cct gac gat 384

Gly Lys Asp Gly Ser Asp Gly Glu Glu Leu Asp Gly Ala Pro Asp Asp

115 120 125

gat gac tat gac act gaa gat tca ttt atc gat gat gct gaa ttg gat 432

Asp Asp Tyr Asp Thr Glu Asp Ser Phe Ile Asp Asp Ala Glu Leu Asp

130 135 140

gag tat ttt gaa gtt gat aat tcg cca att aaa cat gat gga ttt ttt 480

Glu Tyr Phe Glu Val Asp Asn Ser Pro Ile Lys His Asp Gly Phe Phe

145 150 155 160

gtc aat aga gga aag tta gaa cga att gaa cct tca gct aca tcg aac 528

Val Asn Arg Gly Lys Leu Glu Arg Ile Glu Pro Ser Ala Thr Ser Asn

165 170 175

cag cag caa cca aag aaa agg cga agg aag gag tca gca aaa cct tgt 576

Gln Gln Gln Pro Lys Lys Arg Arg Arg Lys Glu Ser Ala Lys Pro Cys

180 185 190

ggc gat gtt gtt gat gta tcc aga aaa cga gcc aag atg gct aag acg 624

Gly Asp Val Val Asp Val Ser Arg Lys Arg Ala Lys Met Ala Lys Thr

195 200 205

gct ggg gga aag gat caa tct gct tct cct ggg ccc tct tcg aag aaa 672

Ala Gly Gly Lys Asp Gln Ser Ala Ser Pro Gly Pro Ser Ser Lys Lys

210 215 220

att tcc aat gat tca aag acg gtg caa gat tcg ttt tcc cct ttg aaa 720

Ile Ser Asn Asp Ser Lys Thr Val Gln Asp Ser Phe Ser Pro Leu Lys

225 230 235 240

gcg caa aat ggc aat gat tcc tta gtt ttg gaa aat gtg aag cat act 768

Ala Gln Asn Gly Asn Asp Ser Leu Val Leu Glu Asn Val Lys His Thr

245 250 255

gat aaa gcg aat cac cag cca atg aat gcc acg agt ccg aag tca aag 816

Asp Lys Ala Asn His Gln Pro Met Asn Ala Thr Ser Pro Lys Ser Lys

260 265 270

gca gct gga tct tct ggc ccc ctt cat ccg aag tgc agc agc aaa agt 864

Ala Ala Gly Ser Ser Gly Pro Leu His Pro Lys Cys Ser Ser Lys Ser

275 280 285

gtt cat gaa caa tct aat tcc cct cca gga aaa tct cgg cca aat gtt 912

Val His Glu Gln Ser Asn Ser Pro Pro Gly Lys Ser Arg Pro Asn Val

290 295 300

tcg gca aaa tca gca gta gtt cgt cag caa gtt aac aat ggc atg cct 960

Ser Ala Lys Ser Ala Val Val Arg Gln Gln Val Asn Asn Gly Met Pro

305 310 315 320

gac ctg gac att gca acg gaa agc aaa aca tct att caa ata tct aaa 1008

Asp Leu Asp Ile Ala Thr Glu Ser Lys Thr Ser Ile Gln Ile Ser Lys

325 330 335

aaa agc ggt tca aat ggc cgg cct aaa tac tcg aca ctt gag aaa gcc 1056

Lys Ser Gly Ser Asn Gly Arg Pro Lys Tyr Ser Thr Leu Glu Lys Ala

340 345 350

atc agg aat ttg gag aag ttg gtc gct gaa tca agg cct cct gct gcc 1104

Ile Arg Asn Leu Glu Lys Leu Val Ala Glu Ser Arg Pro Pro Ala Ala

355 360 365

act gag aat caa gat gcc gat atc tct tcc caa gca gtg aag agg gga 1152

Thr Glu Asn Gln Asp Ala Asp Ile Ser Ser Gln Ala Val Lys Arg Gly

370 375 380

ttg cca gga gat gta aaa ttg cat ctt gct aaa gtt gct aga atc gcg 1200

Leu Pro Gly Asp Val Lys Leu His Leu Ala Lys Val Ala Arg Ile Ala

385 390 395 400

tat gcg agc caa ggt gaa ata tca gga gag tta atc aat cgt ctc atg 1248

Tyr Ala Ser Gln Gly Glu Ile Ser Gly Glu Leu Ile Asn Arg Leu Met

405 410 415

ggc att gtc ggt cat cta ata cag att aga tca ctt aag agg aac ttg 1296

Gly Ile Val Gly His Leu Ile Gln Ile Arg Ser Leu Lys Arg Asn Leu

420 425 430

aaa atc atg att gat tcg atc gtc act gca aat cga gaa aaa gat act 1344

Lys Ile Met Ile Asp Ser Ile Val Thr Ala Asn Arg Glu Lys Asp Thr

435 440 445

aga ttt cag cgg atc aag agt gaa ata act gag atg tta aaa aca caa 1392

Arg Phe Gln Arg Ile Lys Ser Glu Ile Thr Glu Met Leu Lys Thr Gln

450 455 460

gtt cca ctt gtg gaa tcc cag gaa aca aat caa gaa gct gga aca tca 1440

Val Pro Leu Val Glu Ser Gln Glu Thr Asn Gln Glu Ala Gly Thr Ser

465 470 475 480

gac gat ttt cag gat gtt gga tct ctt gga aag tca cct gtg aag aag 1488

Asp Asp Phe Gln Asp Val Gly Ser Leu Gly Lys Ser Pro Val Lys Lys

485 490 495

ttt gtc atg gat gtg gcg ctg gag gaa aaa ttg tgt gat cta tat gac 1536

Phe Val Met Asp Val Ala Leu Glu Glu Lys Leu Cys Asp Leu Tyr Asp

500 505 510

gtg ttt gtt gag gta att aat att ttc cat aac ctt aca taa 1578

Val Phe Val Glu Val Ile Asn Ile Phe His Asn Leu Thr

515 520 525

<210>68

<211>525

<212>PRT

＜213〉Arabidopis thaliana

<400>68

Met Glu Asp Glu Pro Lys Leu Pro Thr Asp Asp Gly Pro Thr Phe Asn

1 5 10 15

Glu Ser Cys Lys Ile Ser Ser Glu Ile Leu Thr Ala Gly Asp Arg Lys

20 25 30

Leu Leu Lys Val Glu Leu Leu Lys Glu Glu Thr Thr Leu Val Ser Trp

35 40 45

Lys Lys Leu Met Asp Glu Ala Ser Lys Glu Asn Gly Gly Leu Phe Val

50 55 60

Ser Ala Pro Glu Arg Leu Leu Asn Ala Asn Pro Asn Leu Glu Phe Arg

65 70 75 80

Leu Ala Pro Gly Ala Gln Thr Glu Asn Glu Met Val Asn Gln Pro His

85 90 95

Pro Asn Arg Leu Asn Ser Val Ile Ala Lys Ile Glu Arg Leu Tyr Met

100 105 110

Gly Lys Asp Gly Ser Asp Gly Glu Glu Leu Asp Gly Ala Pro Asp Asp

115 120 125

Asp Asp Tyr Asp Thr Glu Asp Ser Phe Ile Asp Asp Ala Glu Leu Asp

130 135 140

Glu Tyr Phe Glu Val Asp Asn Ser Pro Ile Lys His Asp Gly Phe Phe

145 150 155 160

Val Asn Arg Gly Lys Leu Glu Arg Ile Glu Pro Ser Ala Thr Ser Asn

165 170 175

Gln Gln Gln Pro Lys Lys Arg Arg Arg Lys Glu Ser Ala Lys Pro Cys

180 185 190

Gly Asp Val Val Asp Val Ser Arg Lys Arg Ala Lys Met Ala Lys Thr

195 200 205

Ala Gly Gly Lys Asp Gln Ser Ala Ser Pro Gly Pro Ser Ser Lys Lys

210 215 220

Ile Ser Asn Asp Ser Lys Thr Val Gln Asp Ser Phe Ser Pro Leu Lys

225 230 235 240

Ala Gln Asn Gly Asn Asp Ser Leu Val Leu Glu Asn Val Lys His Thr

245 250 255

Asp Lys Ala Asn His Gln Pro Met Asn Ala Thr Ser Pro Lys Ser Lys

260 265 270

Ala Ala Gly Ser Ser Gly Pro Leu His Pro Lys Cys Ser Ser Lys Ser

275 280 285

Val His Glu Gln Ser Asn Ser Pro Pro Gly Lys Ser Arg Pro Asn Val

290 295 300

Ser Ala Lys Ser Ala Val Val Arg Gln Gln Val Asn Asn Gly Met Pro

305 310 315 320

Asp Leu Asp Ile Ala Thr Glu Ser Lys Thr Ser Ile Gln Ile Ser Lys

325 330 335

Lys Ser Gly Ser Asn Gly Arg Pro Lys Tyr Ser Thr Leu Glu Lys Ala

340 345 350

Ile Arg Asn Leu Glu Lys Leu Val Ala Glu Ser Arg Pro Pro Ala Ala

355 360 365

Thr Glu Asn Gln Asp Ala Asp Ile Ser Ser Gln Ala Val Lys Arg Gly

370 375 380

Leu Pro Gly Asp Val Lys Leu His Leu Ala Lys Val Ala Arg Ile Ala

385 390 395 400

Tyr Ala Ser Gln Gly Glu Ile Ser Gly Glu Leu Ile Asn Arg Leu Met

405 410 415

Gly Ile Val Gly His Leu Ile Gln Ile Arg Ser Leu Lys Arg Asn Leu

420 425 430

Lys Ile Met Ile Asp Ser Ile Val Thr Ala Asn Arg Glu Lys Asp Thr

435 440 445

Arg Phe Gln Arg Ile Lys Ser Glu Ile Thr Glu Met Leu Lys Thr Gln

450 455 460

Val Pro Leu Val Glu Ser Gln Glu Thr Asn Gln Glu Ala Gly Thr Ser

465 470 475 480

Asp Asp Phe Gln Asp Val Gly Ser Leu Gly Lys Ser Pro Val Lys Lys

485 490 495

Phe Val Met Asp Val Ala Leu Glu Glu Lys Leu Cys Asp Leu Tyr Asp

500 505 510

Val Phe Val Glu Val Ile Asn Ile Phe His Asn Leu Thr

515 520 525

<210>69

<211>2352

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(2352)

<400>69

atg aag acg acg caa ctg ttc aaa ggg gca aat gtt ttt atg tct cgg 48

Met Lys Thr Thr Gln Leu Phe Lys Gly Ala Asn Val Phe Met Ser Arg

1 5 10 15

aat ctg gtg cct cct gaa gtc ttc gac aca ctt ctc gat gct ttc aag 96

Asn Leu Val Pro Pro Glu Val Phe Asp Thr Leu Leu Asp Ala Phe Lys

20 25 30

ctt aac ggt gcc gaa atc ttc ctc tgc tgc gac cca tct cgg agt ggt 144

Leu Asn Gly Ala Glu Ile Phe Leu Cys Cys Asp Pro Ser Arg Ser Gly

35 40 45

ccc tct gat ttc cat gtc atc gct tct ccc gat cat gag aaa ttt aag 192

Pro Ser Asp Phe His Val Ile Ala Ser Pro Asp His Glu Lys Phe Lys

50 55 60

gat ctt aaa gcc aag ggt tgt aac tta ata ggt ccg caa tgt gcg ctc 240

Asp Leu Lys Ala Lys Gly Cys Asn Leu Ile Gly Pro Gln Cys Ala Leu

65 70 75 80

ttc tgt gca aaa gag ggt aga cca ctg cca caa agg gga ttc act tgt 288

Phe Cys Ala Lys Glu Gly Arg Pro Leu Pro Gln Arg Gly Phe Thr Cys

85 90 95

tgc cta gcc atg gat ggt cta aaa gtt ctt gct tct ggt ttt ctg gta 336

Cys Leu Ala Met Asp Gly Leu Lys Val Leu Ala Ser Gly Phe Leu Val

100 105 110

gat gag aag gtc aag atc aag gag ttg gtt act tcc atg ggg ggc gtt 384

Asp Glu Lys Val Lys Ile Lys Glu Leu Val Thr Ser Met Gly Gly Val

115 120 125

tta ctt tcc aga gct tct tct gat gtg aac ttc gtc att gtg aaa aat 432

Leu Leu Ser Arg Ala Ser Ser Asp Val Asn Phe Val Ile Val Lys Asn

130 135 140

gtc ttg gct gcc aag tac aag tgg gcc ctg aat aag aag cca atc gtt 480

Val Leu Ala Ala Lys Tyr Lys Trp Ala Leu Asn Lys Lys Pro Ile Val

145 150 155 160

act ctg aat tgg tta cat cgg tgt tgg aat gag cac cgt gtg gtt cct 528

Thr Leu Asn Trp Leu His Arg Cys Trp Asn Glu His Arg Val Val Pro

165 170 175

cag gaa cca tat aag att cct cct ttt tct gga ttg aca atc tgt gtc 576

Gln Glu Pro Tyr Lys Ile Pro Pro Phe Ser Gly Leu Thr Ile Cys Val

180 185 190

aca aga att cca gca ggt gac aaa tac aaa gtt gct cga aaa tgg ggt 624

Thr Arg Ile Pro Ala Gly Asp Lys Tyr Lys Val Ala Arg Lys Trp Gly

195 200 205

cac att caa att gtc aca cgg aaa tgg ttt cag cag tcc atc gat aaa 672

His Ile Gln Ile Val Thr Arg Lys Trp Phe Gln Gln Ser Ile Asp Lys

210 215 220

aag gtt tgt ctc aat gaa gag tca tat cct gtt ctc ggt tcc ata ccc 720

Lys Val Cys Leu Asn Glu Glu Ser Tyr Pro Val Leu Gly Ser Ile Pro

225 230 235 240

ttg aca aga gga gtg cga gat ttg ggg gtt cat aat ggt cta gaa aag 768

Leu Thr Arg Gly Val Arg Asp Leu Gly Val His Asn Gly Leu Glu Lys

245 250 255

ttt cct tcg gct gca act gcg tcc gcg gca gat tca tat gtt tct tgt 816

Phe Pro Ser Ala Ala Thr Ala Ser Ala Ala Asp Ser Tyr Val Ser Cys

260 265 270

gct cag tct aga gac tca gat ata gaa gct tct gct tca caa aat gtt 864

Ala Gln Ser Arg Asp Ser Asp Ile Glu Ala Ser Ala Ser Gln Asn Val

275 280 285

ttt ccc act tct atg aat ccc agt acc gat gtt aaa gaa cca ggt gga 912

Phe Pro Thr Ser Met Asn Pro Ser Thr Asp Val Lys Glu Pro Gly Gly

290 295 300

ggc cca acg gca agg ccg caa gag caa aac att gat ggt tgt act gcc 960

Gly Pro Thr Ala Arg Pro Gln Glu Gln Asn Ile Asp Gly Cys Thr Ala

305 310 315 320

agg gat tca gaa tcc gaa gac aat gac ttg tac tta tca gat tgt aga 1008

Arg Asp Ser Glu Ser Glu Asp Asn Asp Leu Tyr Leu Ser Asp Cys Arg

325 330 335

att ttc ttg ctt ggt ttt gaa gct tct gaa atg cgt aaa ctt gct aag 1056

Ile Phe Leu Leu Gly Phe Glu Ala Ser Glu Met Arg Lys Leu Ala Lys

340 345 350

ttg gtc cgc aga ggt ggt gga tcc cgg tat atg ctg ctt aac gaa aga 1104

Leu Val Arg Arg Gly Gly Gly Ser Arg Tyr Met Leu Leu Asn Glu Arg

355 360 365

atg act cat att gtt gtt gga act cct tca gag aga gaa gca agg agt 1152

Met Thr His Ile Val Val Gly Thr Pro Ser Glu Arg Glu Ala Arg Ser

370 375 380

gtt gca gct tct ggt gtc att caa gta gtc ata ccc agt tgg ctt gaa 1200

Val Ala Ala Ser Gly Val Ile Gln Val Val Ile Pro Ser Trp Leu Glu

385 390 395 400

gat tgt gat cgt gag aaa aaa gaa atc ccc gtt cat aat ata tat act 1248

Asp Cys Asp Arg Glu Lys Lys Glu Ile Pro Val His Asn Ile Tyr Thr

405 410 415

gct aac cac ttg att ctt cca aga gat tct gca tgc ttg acc aag ggg 1296

Ala Asn His Leu Ile Leu Pro Arg Asp Ser Ala Cys Leu Thr Lys Gly

420 425 430

tca ttt gca agg atg tca agt atg gaa cag act aaa aat act cac gac 1344

Ser Phe Ala Arg Met Ser Ser Met Glu Gln Thr Lys Asn Thr His Asp

435 440 445

cag acc atg gtt ggt tgt tta ctt gct gtt agt agt cat atc ctc tac 1392

Gln Thr Met Val Gly Cys Leu Leu Ala Val Ser Ser His Ile Leu Tyr

450 455 460

tca cct ctt ccc tgc cag aca cct ttg cct gga ttc gaa agc ctt tgc 1440

Ser Pro Leu Pro Cys Gln Thr Pro Leu Pro Gly Phe Glu Ser Leu Cys

465 470 475 480

ata tgt agt tcc caa cat aat gag aag aat gta gaa ctc ctg aga aat 1488

Ile Cys Ser Ser Gln His Asn Glu Lys Asn Val Glu Leu Leu Arg Asn

485 490 495

ttg agt gtc gtt ctt gga gca gat ttt gtg gaa aga cta acc agg aaa 1536

Leu Ser Val Val Leu Gly Ala Asp Phe Val Glu Arg Leu Thr Arg Lys

500 505 510

gtg act cac ttg ata tgc aac ttt gca aaa gga gat aag tat gtg aga 1584

Val Thr His Leu Ile Cys Asn Phe Ala Lys Gly Asp Lys Tyr Val Arg

515 520 525

gct tcc aag tgg gga ata att tcc gtg aca cct gac tgg ctt tat gaa 1632

Ala Ser Lys Trp Gly Ile Ile Ser Val Thr Pro Asp Trp Leu Tyr Glu

530 535 540

tgt gtt aga cag aat caa gtt gtt tgt aca gat aac ttc cat cca agg 1680

Cys Val Arg Gln Asn Gln Val Val Cys Thr Asp Asn Phe His Pro Arg

545 550 555 560

gaa ttg acc act caa gat cga gaa gca ggg tct cag ttt cat aca cag 1728

Glu Leu Thr Thr Gln Asp Arg Glu Ala Gly Ser Gln Phe His Thr Gln

565 570 575

ttt gta cca atg gcc tca agg gac agt atg tct cta cct gta agt cac 1776

Phe Val Pro Met Ala Ser Arg Asp Ser Met Ser Leu Pro Val Ser His

580 585 590

tct gaa gac agg gaa aaa att caa agt ttt gct ggc aaa agt ggt tgc 1824

Ser Glu Asp Arg Glu Lys Ile Gln Ser Phe Ala Gly Lys Ser Gly Cys

595 600 605

ggg aaa ggt gaa gta tat aac aga ctt gga gaa att gga aag gaa caa 1872

Gly Lys Gly Glu Val Tyr Asn Arg Leu Gly Glu Ile Gly Lys Glu Gln

610 615 620

act ttt ccg tct aag aag gca aaa ctt ttg aga gat ggt caa gaa agt 1920

Thr Phe Pro Ser Lys Lys Ala Lys Leu Leu Arg Asp Gly Gln Glu Ser

625 630 635 640

gat gtg ttt cct gtg aga gaa ctt cca agc aat tgt gat cgt cct tcg 1968

Asp Val Phe Pro Val Arg Glu Leu Pro Ser Asn Cys Asp Arg Pro Ser

645 650 655

cat tct gga gat ggc att gtg act gga tat gat gta gca agt ggt cgt 2016

His Ser Gly Asp Gly Ile Val Thr Gly Tyr Asp Val Ala Ser Gly Arg

660 665 670

gaa gtt cca gat gtg gct gat act att gag gat ctg tta gag cag aca 2064

Glu Val Pro Asp Val Ala Asp Thr Ile Glu Asp Leu Leu Glu Gln Thr

675 680 685

agc aaa att caa gat cag aag tct cct ggg agg att tta gaa aag act 2112

Ser Lys Ile Gln Asp Gln Lys Ser Pro Gly Arg Ile Leu Glu Lys Thr

690 695 700

gta tcc tta aat gaa caa tac aac act ggg aat cac tct gtc act ggc 2160

Val Ser Leu Asn Glu Gln Tyr Asn Thr Gly Asn His Ser Val Thr Gly

705 710 715 720

ctg tct aga cac tgg ata aac agg gtc cat aag aat gac gac atg ggc 2208

Leu Ser Arg His Trp Ile Asn Arg Val His Lys Asn Asp Asp Met Gly

725 730 735

agt cct cca gga gat gca act act gac act tac gga aac ttt agt gag 2256

Ser Pro Pro Gly Asp Ala Thr Thr Asp Thr Tyr Gly Asn Phe Ser Glu

740 745 750

acg cag aca gaa tca cag gtt gtt ggt tac gag gaa gat ctt tca gga 2304

Thr Gln Thr Glu Ser Gln Val Val Gly Tyr Glu Glu Asp Leu Ser Gly

755 760 765

agg cag atg ctt ata gac aga gtt aga aca cga agc agc tta aca taa 2352

Arg Gln Met Leu Ile Asp Arg Val Arg Thr Arg Ser Ser Leu Thr

770 775 780

<210>70

<211>783

<212>PRT

＜213〉Arabidopis thaliana

<400>70

Met Lys Thr Thr Gln Leu Phe Lys Gly Ala Asn Val Phe Met Ser Arg

1 5 10 15

Asn Leu Val Pro Pro Glu Val Phe Asp Thr Leu Leu Asp Ala Phe Lys

20 25 30

Leu Asn Gly Ala Glu Ile Phe Leu Cys Cys Asp Pro Ser Arg Ser Gly

35 40 45

Pro Ser Asp Phe His Val Ile Ala Ser Pro Asp His Glu Lys Phe Lys

50 55 60

Asp Leu Lys Ala Lys Gly Cys Asn Leu Ile Gly Pro Gln Cys Ala Leu

65 70 75 80

Phe Cys Ala Lys Glu Gly Arg Pro Leu Pro Gln Arg Gly Phe Thr Cys

85 90 95

Cys Leu Ala Met Asp Gly Leu Lys Val Leu Ala Ser Gly Phe Leu Val

100 105 110

Asp Glu Lys Val Lys Ile Lys Glu Leu Val Thr Ser Met Gly Gly Val

115 120 125

Leu Leu Ser Arg Ala Ser Ser Asp Val Asn Phe Val Ile Val Lys Asn

130 135 140

Val Leu Ala Ala Lys Tyr Lys Trp Ala Leu Asn Lys Lys Pro Ile Val

145 150 155 160

Thr Leu Asn Trp Leu His Arg Cys Trp Asn Glu His Arg Val Val Pro

165 170 175

Gln Glu Pro Tyr Lys Ile Pro Pro Phe Ser Gly Leu Thr Ile Cys Val

180 185 190

Thr Arg Ile Pro Ala Gly Asp Lys Tyr Lys Val Ala Arg Lys Trp Gly

195 200 205

His Ile Gln Ile Val Thr Arg Lys Trp Phe Gln Gln Ser Ile Asp Lys

210 215 220

Lys Val Cys Leu Asn Glu Glu Ser Tyr Pro Val Leu Gly Ser Ile Pro

225 230 235 240

Leu Thr Arg Gly Val Arg Asp Leu Gly Val His Asn Gly Leu Glu Lys

245 250 255

Phe Pro Ser Ala Ala Thr Ala Ser Ala Ala Asp Ser Tyr Val Ser Cys

260 265 270

Ala Gln Ser Arg Asp Ser Asp Ile Glu Ala Ser Ala Ser Gln Asn Val

275 280 285

Phe Pro Thr Ser Met Asn Pro Ser Thr Asp Val Lys Glu Pro Gly Gly

290 295 300

Gly Pro Thr Ala Arg Pro Gln Glu Gln Asn Ile Asp Gly Cys Thr Ala

305 310 315 320

Arg Asp Ser Glu Ser Glu Asp Asn Asp Leu Tyr Leu Ser Asp Cys Arg

325 330 335

Ile Phe Leu Leu Gly Phe Glu Ala Ser Glu Met Arg Lys Leu Ala Lys

340 345 350

Leu Val Arg Arg Gly Gly Gly Ser Arg Tyr Met Leu Leu Asn Glu Arg

355 360 365

Met Thr His Ile Val Val Gly Thr Pro Ser Glu Arg Glu Ala Arg Ser

370 375 380

Val Ala Ala Ser Gly Val Ile Gln Val Val Ile Pro Ser Trp Leu Glu

385 390 395 400

Asp Cys Asp Arg Glu Lys Lys Glu Ile Pro Val His Asn Ile Tyr Thr

405 410 415

Ala Asn His Leu Ile Leu Pro Arg Asp Ser Ala Cys Leu Thr Lys Gly

420 425 430

Ser Phe Ala Arg Met Ser Ser Met Glu Gln Thr Lys Asn Thr His Asp

435 440 445

Gln Thr Met Val Gly Cys Leu Leu Ala Val Ser Ser His Ile Leu Tyr

450 455 460

Ser Pro Leu Pro Cys Gln Thr Pro Leu Pro Gly Phe Glu Ser Leu Cys

465 470 475 480

Ile Cys Ser Ser Gln His Asn Glu Lys Asn Val Glu Leu Leu Arg Asn

485 490 495

Leu Ser Val Val Leu Gly Ala Asp Phe Val Glu Arg Leu Thr Arg Lys

500 505 510

Val Thr His Leu Ile Cys Asn Phe Ala Lys Gly Asp Lys Tyr Val Arg

515 520 525

Ala Ser Lys Trp Gly Ile Ile Ser Val Thr Pro Asp Trp Leu Tyr Glu

530 535 540

Cys Val Arg Gln Asn Gln Val Val Cys Thr Asp Asn Phe His Pro Arg

545 550 555 560

Glu Leu Thr Thr Gln Asp Arg Glu Ala Gly Ser Gln Phe His Thr Gln

565 570 575

Phe Val Pro Met Ala Ser Arg Asp Ser Met Ser Leu Pro Val Ser His

580 585 590

Ser Glu Asp Arg Glu Lys Ile Gln Ser Phe Ala Gly Lys Ser Gly Cys

595 600 605

Gly Lys Gly Glu Val Tyr Asn Arg Leu Gly Glu Ile Gly Lys Glu Gln

610 615 620

Thr Phe Pro Ser Lys Lys Ala Lys Leu Leu Arg Asp Gly Gln Glu Ser

625 630 635 640

Asp Val Phe Pro Val Arg Glu Leu Pro Ser Asn Cys Asp Arg Pro Ser

645 650 655

His Ser Gly Asp Gly Ile ValThr Gly Tyr Asp ValAla Ser Gly Arg

660 665 670

Glu Val Pro Asp Val Ala Asp Thr Ile Glu Asp Leu Leu Glu Gln Thr

675 680 685

Ser Lys Ile Gln Asp Gln Lys Ser Pro Gly Arg Ile Leu Glu Lys Thr

690 695 700

Val Ser Leu Asn Glu Gln Tyr Asn Thr Gly Asn His Ser Val Thr Gly

705 710 715 720

Leu Ser Arg His Trp Ile Asn Arg Val His Lys Asn Asp Asp Met Gly

725 730 735

Ser Pro Pro Gly Asp Ala Thr Thr Asp Thr Tyr Gly Asn Phe Ser Glu

740 745 750

Thr Gln Thr Glu Ser Gln Val Val Gly Tyr Glu Glu Asp Leu Ser Gly

755 760 765

Arg Gln Met Leu Ile Asp Arg Val Arg Thr Arg Ser Ser Leu Thr

770 775 780

<210>71

<211>816

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1).. (816)

<400>71

atg caa cgt ttc tgt ata aag aca tct agc att gag ata gat cca ctt 48

Met Gln Arg Phe Cys Ile Lys Thr Ser Ser Ile Glu Ile Asp Pro Leu

1 5 10 15

gct gcg cct tcc gct ttc gtt tca ttc ctg atg tcg gtg agg gga aat 96

Ala Ala Pro Ser Ala Phe Val Ser Phe Leu Met Ser Val Arg Gly Asn

20 25 30

gaa ctt gac aga tac gat gca gag aat ctt gca cat gct cta ctt cat 144

Glu Leu Asp Arg Tyr Asp Ala Glu Asn Leu Ala His Ala Leu Leu His

35 40 45

atg cct ggc ttg gaa tct ctt gac ctg agc ggg aac ccc att gaa gac 192

Met Pro Gly Leu Glu Ser Leu Asp Leu Ser Gly Asn Pro Ile Glu Asp

50 55 60

agt ggg atc aga agc tta ata tct tac ttc aca aag aat ccg gat tct 240

Ser Gly Ile Arg Ser Leu Ile Ser Tyr Phe Thr Lys Asn Pro Asp Ser

65 70 75 80

cgt tta gcc gat ctg aat ttg gag aac tgt gag cta tca tgt tgt gga 288

Arg Leu Ala Asp Leu Asn Leu Glu Asn Cys Glu Leu Ser Cys Cys Gly

85 90 95

gtt att gag ttt ctt gat acc ctg tcg atg ctg gag aaa cct tta aag 336

Val Ile Glu Phe Leu Asp Thr Leu Ser Met Leu Glu Lys Pro Leu Lys

100 105 110

ttc ctg tct gtt gca gat aat gcc ctc gga agc gag gtt gca gag gct 384

Phe Leu Ser Val Ala Asp Asn Ala Leu Gly Ser Glu Val Ala Glu Ala

115 120 125

gta gta aac tct ttc aca atc tcc atc gag tcg ctc aat att atg ggt 432

Val Val Asn Ser Phe Thr Ile Ser Ile Glu Ser Leu Asn Ile Met Gly

130 135 140

ata gga cta ggt cct ctc ggg ttt ctt gca tta ggc aga aaa ctt gaa 480

Ile Gly Leu Gly Pro Leu Gly Phe Leu Ala Leu Gly Arg Lys Leu Glu

145 150 155 160

aaa gtg tcg aag aag ctg ctg agt att aat ata agc aaa aac cgt gga 528

Lys Val Ser Lys Lys Leu Leu Ser Ile Asn Ile Ser Lys Asn Arg Gly

165 170 175

gga cta gag acc gct aga ttc ctg tca aag ctc ata ccc ttg gca cca 576

Gly Leu Glu Thr Ala Arg Phe Leu Ser Lys Leu Ile Pro Leu Ala Pro

180 185 190

aaa ctc atc tca atc gac gca tcc tac aat ctt atg cca cct gaa gcc 624

Lys Leu Ile Ser Ile Asp Ala Ser Tyr Asn Leu Met Pro Pro Glu Ala

195 200 205

ttg ctc atg cta tgt gat tcc ctg aga act gca aaa ggt gat ctc aaa 672

Leu Leu Met Leu Cys Asp Ser Leu Arg Thr Ala Lys Gly Asp Leu Lys

210 215 220

cgt ctt gac atg act ggg aat agt tgc atc agc cac gaa gct gac cat 720

Arg Leu Asp Met Thr Gly Asn Ser Cys Ile Ser His Glu Ala Asp His

225 230 235 240

tct tct cta ctc cat gaa ttt caa cac aac gga gaa ccc atc ttc gtt 768

Ser Ser Leu Leu His Glu Phe Gln His Asn Gly Glu Pro Ile Phe Val

245 250 255

tta cct tca tcc tcg gtt tca cat gtt cct tac gat gat gac ccg tag 816

Leu Pro Ser Ser Ser Val Ser His Val Pro Tyr Asp Asp Asp Pro

260 265 270

<210>72

<211>271

<212>PRT

＜213〉Arabidopis thaliana

<400>72

Met Gln Arg Phe Cys Ile Lys Thr Ser Ser Ile Glu Ile Asp Pro Leu

1 5 10 15

Ala Ala Pro Ser Ala Phe Val Ser Phe Leu Met Ser Val Arg Gly Asn

20 25 30

Glu Leu Asp Arg Tyr Asp Ala Glu Asn Leu Ala His Ala Leu Leu His

35 40 45

Met Pro Gly Leu Glu Ser Leu Asp Leu Ser Gly Asn Pro Ile Glu Asp

50 55 60

Ser Gly Ile Arg Ser Leu Ile Ser Tyr Phe Thr Lys Asn Pro Asp Ser

65 70 75 80

Arg Leu Ala Asp Leu Asn Leu Glu Asn Cys Glu Leu Ser Cys Cys Gly

85 90 95

Val Ile Glu Phe Leu Asp Thr Leu Ser Met Leu Glu Lys Pro Leu Lys

100 105 110

Phe Leu Ser Val Ala Asp Asn Ala Leu Gly Ser Glu Val Ala Glu Ala

115 120 125

Val Val Asn Ser Phe Thr Ile Ser Ile Glu Ser Leu Asn Ile Met Gly

130 135 140

Ile Gly Leu Gly Pro Leu Gly Phe Leu Ala Leu Gly Arg Lys Leu Glu

145 150 155 160

Lys Val Ser Lys Lys Leu Leu Ser Ile Asn Ile Ser Lys Asn Arg Gly

165 170 175

Gly Leu Glu Thr Ala Arg Phe Leu Ser Lys Leu Ile Pro Leu Ala Pro

180 185 190

Lys Leu Ile Ser Ile Asp Ala Ser Tyr Asn Leu Met Pro Pro Glu Ala

195 200 205

Leu Leu Met Leu Cys Asp Ser Leu Arg Thr Ala Lys Gly Asp Leu Lys

210 215 220

Arg Leu Asp Met Thr Gly Asn Ser Cys Ile Ser His Glu Ala Asp His

225 230 235 240

Ser Ser Leu Leu His Glu Phe Gln His Asn Gly Glu Pro Ile Phe Val

245 250 255

Leu Pro Ser Ser Ser Val Ser His Val Pro Tyr Asp Asp Asp Pro

260 265 270

<210>73

<211>600

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(600)

<400>73

atg gag gaa gct tta gaa atg gcg aga gcc aag gat aca aaa gag cgc 48

Met Glu Glu Ala Leu Glu Met Ala Arg Ala Lys Asp Thr Lys Glu Arg

1 5 10 15

atg gct gct gtt gaa agg ctg cat caa ctt ctc gaa gct tct agg aag 96

Met Ala Ala Val Glu Arg Leu His Gln Leu Leu Glu Ala Ser Arg Lys

20 25 30

agt ttg agt cct gcg gaa gtg acg tca ctt gtt gat tct tgt ttg gat 144

Ser Leu Ser Pro Ala Glu Val Thr Ser Leu Val Asp Ser Cys Leu Asp

35 40 45

ctc ctt aag gat agt aat ttt aga gtc tct caa ggt gct ctt caa gct 192

Leu Leu Lys Asp Ser Asn Phe Arg Val Ser Gln Gly Ala Leu Gln Ala

50 55 60

ctt gct tct gct gct gtc ctc gct ggt gag cat ttg aag ctt cat ttg 240

Leu Ala Ser Ala Ala Val Leu Ala Gly Glu His Leu Lys Leu His Leu

65 70 75 80

aat gct ctt gtt cct gct gtt gtc gag cgg ctt ggt gat agt aag caa 288

Asn Ala Leu Val Pro Ala ValVal Glu Arg Leu Gly Asp Ser Lys Gln

85 90 95

cct gtt aga gat gct gcg agg cgt ctg ttg acg act ctc atg gag gtt 336

Pro Val Arg Asp Ala Ala Arg Arg Leu Leu Thr Thr Leu Met Glu Val

100 105 110

tca tct ccg acg att ata gtg gag aga gct ggt tcg tat gct tgg atg 384

Ser Ser Pro Thr Ile Ile Val Glu Arg Ala Gly Ser Tyr Ala Trp Met

115 120 125

cat aag agt tgg aga gtt agg gaa gag ttt gcg cgt act gtt aca tcg 432

His Lys Ser Trp Arg Val Arg Glu Glu Phe Ala Arg Thr Val Thr Ser

130 135 140

gcg att ggt ctt ttc gca tct acg gaa ctt cct ctt cag cgt gtt ata 480

Ala Ile Gly Leu Phe Ala Ser Thr Glu Leu Pro Leu Gln Arg Val Ile

145 150 155 160

ctt gct ccg gta tct atc tct ctc cct tta aaa gct att tgg atc atg 528

Leu Ala Pro Val Ser Ile Ser Leu Pro Leu Lys Ala Ile Trp Ile Met

165 170 175

ttt ttc ttg tat tgg atc gaa ttt gat tgc ggt tat ttt gtt tgt tta 576

Phe Phe Leu Tyr Trp Ile Glu Phe Asp Cys Gly Tyr Phe Val Cys Leu

180 185 190

tca gat act tca gat gtt aaa tga 600

Ser Asp Thr Ser Asp Val Lys

195

<210>74

<211>199

<212>PRT

＜213〉Arabidopis thaliana

<400>74

Met Glu Glu Ala Leu Glu Met Ala Arg Ala Lys Asp Thr Lys Glu Arg

1 5 10 15

Met Ala Ala Val Glu Arg Leu His Gln Leu Leu Glu Ala Ser Arg Lys

20 25 30

Ser Leu Ser Pro Ala Glu Val Thr Ser Leu Val Asp Ser Cys Leu Asp

35 40 45

Leu Leu Lys Asp Ser Asn Phe Arg Val Ser Gln Gly Ala Leu Gln Ala

50 55 60

Leu Ala Ser Ala Ala Val Leu Ala Gly Glu His Leu Lys Leu His Leu

65 70 75 80

Asn Ala Leu Val Pro Ala Val Val Glu Arg Leu Gly Asp Ser Lys Gln

85 90 95

Pro Val Arg Asp Ala Ala Arg Arg Leu Leu Thr Thr Leu Met Glu Val

100 105 110

Ser Ser Pro Thr Ile Ile Val Glu Arg Ala Gly Ser Tyr Ala Trp Met

115 120 125

His Lys Ser Trp Arg Val Arg Glu Glu Phe Ala Arg Thr Val Thr Ser

130 135 140

Ala Ile Gly Leu Phe Ala Ser Thr Glu Leu Pro Leu Gln Arg Val Ile

145 150 155 160

Leu Ala Pro Val Ser Ile Ser Leu Pro Leu Lys Ala Ile Trp Ile Met

165 170 175

Phe Phe Leu Tyr Trp Ile Glu Phe Asp Cys Gly Tyr Phe Val Cys Leu

180 185 190

Ser Asp Thr Ser Asp Val Lys

195

<210>75

<211>852

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(852)

<400>75

atg atg atg ggc aaa gaa gat cta ggt ttg agc cta agc tta ggg ttt 48

Met Met Met Gly Lys Glu Asp Leu Gly Leu Ser Leu Ser Leu Gly Phe

1 5 10 15

tca caa aat cac aat cct ctt cag atg aat ctg aat cct aac tct tca 96

Ser Gln Asn His Asn Pro Leu Gln Met Asn Leu Asn Pro Asn Ser Ser

20 25 30

tta tca aac aat ctc cag aga ctc cca tgg aac caa aca ttc gat cct 144

Leu Ser Asn Asn Leu Gln Arg Leu Pro Trp Asn Gln Thr Phe Asp Pro

35 40 45

aca tca gat ctt cgc aag ata gac gtg aac agt ttt cca tca acg gtt 192

Thr Ser Asp Leu Arg Lys Ile Asp Val Asn Ser Phe Pro Ser Thr Val

50 55 60

aac tgc gag gaa gac aca gga gtt tcg tca cca aac agt acg atc tca 240

Asn Cys Glu Glu Asp Thr Gly Val Ser Ser Pro Asn Ser Thr Ile Ser

65 70 75 80

agc acc att agc ggg aag aga agt gag aga gaa gga atc tcc gga acc 288

Ser Thr Ile Ser Gly Lys Arg Ser Glu Arg Glu Gly Ile Ser Gly Thr

85 90 95

ggc gtt ggc tcc ggc gac gat cac gac gag atc act ccg gat cga ggg 336

Gly Val Gly Ser Gly Asp Asp His Asp Glu Ile Thr Pro Asp Arg Gly

100 105 110

tac tca cgt gga acc tca gat gaa gaa gaa gac ggg ggc gaa acg tcg 384

Tyr Ser Arg Gly Thr Ser Asp Glu Glu Glu Asp Gly Gly Glu Thr Ser

115 120 125

agg aag aag ctc agg tta tca aaa gat cag tct gct ttt ctc gaa gag 432

Arg Lys Lys Leu Arg Leu Ser Lys Asp Gln Ser Ala Phe Leu Glu Glu

130 135 140

act ttc aaa gaa cac aac act ctc aat ccc aaa cag aag cta gct ttg 480

Thr Phe Lys Glu His Asn Thr Leu Asn Pro Lys Gln Lys Leu Ala Leu

145 150 155 160

gct aag aag ctg aac ttg acg gca aga caa gtg gaa gtg tgg ttc caa 528

Ala Lys Lys Leu Asn Leu Thr Ala Arg Gln Val Glu Val Trp Phe Gln

165 170 175

aac aga aga gct aga acc aag tta aag caa acg gag gta gat tgc gaa 576

Asn Arg Arg Ala Arg Thr Lys Leu Lys Gln Thr Glu Val Asp Cys Glu

180 185 190

tac ttg aaa cgg tgc gta gag aag cta acg gaa gag aac cgg aga ctt 624

Tyr Leu Lys Arg Cys Val Glu Lys Leu Thr Glu Glu Asn Arg Arg Leu

195 200 205

cag aaa gag gct atg gag ctt cga act ctc aag ctg tct cca caa ttc 672

Gln Lys Glu Ala Met Glu Leu Arg Thr Leu Lys Leu Ser Pro Gln Phe

210 215 220

tac ggt cag atg act cca cca act aca ctc atc atg tgt cct tcg tgc 720

Tyr Gly Gln Met Thr Pro Pro Thr Thr Leu Ile Met Cys Pro Ser Cys

225 230 235 240

gag cgt gtg ggt ggc cca tca tca tcg aac cat cac cac aat cac agg 768

Glu Arg Val Gly Gly Pro Ser Ser Ser Asn His His His Asn His Arg

245 250 255

ccc gtt tct atc aat ccg tgg gtt gct tgt gct ggt cag gtg gct cat 816

Pro Val Ser Ile Asn Pro Trp Val Ala Cys Ala Gly Gln Val Ala His

260 265 270

ggg ctg aat ttt gaa gcc ttg cgt cca cga tcg tga 852

Gly Leu Asn Phe Glu Ala Leu Arg Pro Arg Ser

275 280

<210>76

<211>283

<212>PRT

＜213〉Arabidopis thaliana

<400>76

Met Met Met Gly Lys Glu Asp Leu Gly Leu Ser Leu Ser Leu Gly Phe

1 5 10 15

Ser Gln Asn His Asn Pro Leu Gln Met Asn Leu Asn Pro Asn Ser Ser

20 25 30

Leu Ser Asn Asn Leu Gln Arg Leu Pro Trp Asn Gln Thr Phe Asp Pro

35 40 45

Thr Ser Asp Leu Arg Lys Ile Asp Val Asn Ser Phe Pro Ser Thr Val

50 55 60

Asn Cys Glu Glu Asp Thr Gly Val Ser Ser Pro Asn Ser Thr Ile Ser

65 70 75 80

Ser Thr Ile Ser Gly Lys Arg Ser Glu Arg Glu Gly Ile Ser Gly Thr

85 90 95

Gly Val Gly Ser Gly Asp Asp His Asp Glu Ile Thr Pro Asp Arg Gly

100 105 110

Tyr Ser Arg Gly Thr Ser Asp Glu Glu Glu Asp Gly Gly Glu Thr Ser

115 120 125

Arg Lys Lys Leu Arg Leu Ser Lys Asp Gln Ser Ala Phe Leu Glu Glu

130 135 140

Thr Phe Lys Glu His Asn Thr Leu Asn Pro Lys Gln Lys Leu Ala Leu

145 150 155 160

Ala Lys Lys Leu Asn Leu Thr Ala Arg Gln Val Glu Val Trp Phe Gln

165 170 175

Asn Arg Arg Ala Arg Thr Lys Leu Lys Gln Thr Glu Val Asp Cys Glu

180 185 190

Tyr Leu Lys Arg Cys Val Glu Lys Leu Thr Glu Glu Asn Arg Arg Leu

195 200 205

Gln Lys Glu Ala Met Glu Leu Arg Thr Leu Lys Leu Ser Pro Gln Phe

210 215 220

Tyr Gly Gln Met Thr Pro Pro Thr Thr Leu Ile Met Cys Pro Ser Cys

225 230 235 240

Glu Arg Val Gly Gly Pro Ser Ser Ser Asn His His His Asn His Arg

245 250 255

Pro Val Ser Ile Asn Pro Trp Val Ala Cys Ala Gly Gln Val Ala His

260 265 270

Gly Leu Asn Phe Glu Ala Leu Arg Pro Arg Ser

275 280

<210>77

<211>4125

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(4125)

<400>77

atg aga aat tgt ctt cca atg gaa ttg aat ctg cgc aag ggc gac aag 48

Met Arg Asn Cys Leu Pro Met Glu Leu Asn Leu Arg Lys Gly Asp Lys

1 5 10 15

gtt tgg gtc gaa gat aag gat ttg gct tgg att gct gct gat gtc ctc 96

Val Trp Val Glu Asp Lys Asp Leu Ala Trp Ile Ala Ala Asp Val Leu

20 25 30

gat tct ttt gat aac aaa ctc cat gtt gaa act tct act ggg aag aag 144

Asp Ser Phe Asp Asn Lys Leu His Val Glu Thr Ser Thr Gly Lys Lys

35 40 45

cta ttt cgg agg gat cct gac gat gaa gag cat aat gga gtg gat gat 192

Leu Phe Arg Arg Asp Pro Asp Asp Glu Glu His Asn Gly Val Asp Asp

50 55 60

atg acc aaa ctg aca tac ttg cac gaa gct ggt gtt ctt tat aat cta 240

Met Thr Lys Leu Thr Tyr Leu His Glu Ala Gly Val Leu Tyr Asn Leu

65 70 75 80

cag agg aga tat gct ctg aat gat atc tat aca tac act gga agc att 288

Gln Arg Arg Tyr Ala Leu Asn Asp Ile Tyr Thr Tyr Thr Gly Ser Ile

85 90 95

ctg atc gct gtt aat cca ttc aaa aag ctt cca cat ctc tac aat ggg 336

Leu Ile Ala Val Asn Pro Phe Lys Lys Leu Pro His Leu Tyr Asn Gly

100 105 110

cac atg atg gaa cag tac atg gga gca cca ttc ggt gag ctc agt cct 384

His Met Met Glu Gln Tyr Met Gly Ala Pro Phe Gly Glu Leu Ser Pro

115 120 125

cat gtt ttt gca gtt tct gat gtt gca tac aga gca atg att gac gac 432

His Val Phe Ala Val Ser Asp Val Ala Tyr Arg Ala Met Ile Asp Asp

130 135 140

agt cga agt cag tca ata ctt gtt agc ggt gaa agt gga gct gga aaa 480

Ser Arg Ser Gln Ser Ile Leu Val Ser Gly Glu Ser Gly Ala Gly Lys

145 150 155 160

act gag aca acc aaa cta atc atg cag tat ctt aca ttt gtt ggg gga 528

Thr Glu Thr Thr Lys Leu Ile Met Gln Tyr Leu Thr Phe Val Gly Gly

165 170 175

cgt gct act gac gat gat aga agt gtt gag cag caa gtc ctt gaa tca 576

Arg Ala Thr Asp Asp Asp Arg Ser Val Glu Gln Gln Val Leu Glu Ser

180 185 190

aat cct ctc ttg gaa gca ttt ggc aat gca aaa aca gtt aga aat gat 624

Asn Pro Leu Leu Glu Ala Phe Gly Asn Ala Lys Thr Val Arg Asn Asp

195 200 205

aat tcc agc cgt ttt gga aag ttt gtc gaa atc cag ttt gac aca aat 672

Asn Ser Ser Arg Phe Gly Lys Phe Val Glu Ile Gln Phe Asp Thr Asn

210 215 220

ggt aga ata tct ggt gcc gca atc aga acc tat ctt ctg gag aga tca 720

Gly Arg Ile Ser Gly Ala Ala Ile Arg Thr Tyr Leu Leu Glu Arg Ser

225 230 235 240

cgt gtt gtc cgg ata aca gac ccc gag agg aat tat cat tgt ttt tat 768

Arg Val Val Arg Ile Thr Asp Pro Glu Arg Asn Tyr His Cys Phe Tyr

245 250 255

caa ttg tgc gct tcg ggg aat gac gct gag aaa tat aaa cta agc aac 816

Gln Leu Cys Ala Ser Gly Asn Asp Ala Glu Lys Tyr Lys Leu Ser Asn

260 265 270

cct cgt caa ttt cat tat cta aat caa agc aag acc tat gaa tta gaa 864

Pro Arg Gln Phe His Tyr Leu Asn Gln Ser Lys Thr Tyr Glu Leu Glu

275 280 285

gga gtc agc agc gca gaa gag tat aag aat aca agg agg gca atg gat 912

Gly Val Ser Ser Ala Glu Glu Tyr Lys Asn Thr Arg Arg Ala Met Asp

290 295 300

att gtg ggc ata agt cag gat gag cag gaa ggg ata ttt cgc aca ctt 960

Ile Val Gly Ile Ser Gln Asp Glu Gln Glu Gly Ile Phe Arg Thr Leu

305 310 315 320

gct gcg att cta cat ctt gga aat gtt gag ttt tcc tca ggg aga gag 1008

Ala Ala Ile Leu His Leu Gly Asn Val Glu Phe Ser Ser Gly Arg Glu

325 330 335

cac gac tct tca gtg gta aag gat ccg gaa tct aga cat cat ctg cag 1056

His Asp Ser Ser Val Val Lys Asp Pro Glu Ser Arg His His Leu Gln

340 345 350

atg gct gct gat ctt ttc aag tgt gat gca aat ctt ttg ctg gct tcg 1104

Met Ala Ala Asp Leu Phe Lys Cys Asp Ala Asn Leu Leu Leu Ala Ser

355 360 365

ctc tgc aca cgt tca att ctg acc cgt gaa ggt atc att atc aaa gca 1152

Leu Cys Thr Arg Ser Ile Leu Thr Arg Glu Gly Ile Ile Ile Lys Ala

370 375 380

ctt gac cct aat gct gct gtt act agc cgg gat acc ctc gcg aag act 1200

Leu Asp Pro Asn Ala Ala Val Thr Ser Arg Asp Thr Leu Ala Lys Thr

385 390 395 400

gtt tac gcc cat cta ttt gac tgg ctg gtt gat aag atc aat aag tct 1248

Val Tyr Ala His Leu Phe Asp Trp Leu Val Asp Lys Ile Asn Lys Ser

405 410 415

gtt ggg caa gat cca gaa tct cgt ttt caa ata gga gtc ctg gac att 1296

Val Gly Gln Asp Pro Glu Ser Arg Phe Gln Ile Gly Val Leu Asp Ile

420 425 430

tat ggc ttt gaa tgt ttt aag aat aac agt ttt gaa caa ttt tgc atc 1344

Tyr Gly Phe Glu Cys Phe Lys Asn Asn Ser Phe Glu Gln Phe Cys Ile

435 440 445

aac ttt gca aat gaa aag ctg cag caa cat ttc aac gag cat gta ttc 1392

Asn Phe Ala Asn Glu Lys Leu Gln Gln His Phe Asn Glu His Val Phe

450 455 460

aag atg gag cag gat gag tac aga aaa gaa gaa att aat tgg agt tat 1440

Lys Met Glu Gln Asp Glu Tyr Arg Lys Glu Glu Ile Asn Trp Ser Tyr

465 470 475 480

atc gag ttt att gac aac caa gat gtc ttg gac ctt att gag aag aag 1488

Ile Glu Phe Ile Asp Asn Gln Asp Val Leu Asp Leu Ile Glu Lys Lys

485 490 495

cct att ggg gtg att gca ctc tta gat gaa gct tgc atg ttt cct aga 1536

Pro Ile Gly Val Ile Ala Leu Leu Asp Glu Ala Cys Met Phe Pro Arg

500 505 510

tca act cat gag tca ttt tca atg aag ctg ttt cag aac ttt aga ttt 1584

Ser Thr His Glu Ser Phe Ser Met Lys Leu Phe Gln Asn Phe Arg Phe

515 520 525

cat ccg aga ttg gag aag cca aaa ttt tca gag acg gat ttt act ctc 1632

His Pro Arg Leu Glu Lys Pro Lys Phe Ser Glu Thr Asp Phe Thr Leu

530 535 540

tct cat tat gct ggc aag gca acc ttt ttg gat aaa aac cgt gat tat 1680

Ser His Tyr Ala Gly Lys Ala Thr Phe Leu Asp Lys Asn Arg Asp Tyr

545 550 555 560

act ata gtg gag cat tgc aat ctg ctg tct tcc tcc aaa tgc cct ttt 1728

Thr Ile Val Glu His Cys Asn Leu Leu Ser Ser Ser Lys Cys Pro Phe

565 570 575

gtt gct gga att ttc ccc tca gcc ccg gag gag tct acc aga tct tct 1776

Val Ala Gly Ile Phe Pro Ser Ala Pro Glu Glu Ser Thr Arg Ser Ser

580 585 590

tac aaa ttt tct tct gta tct tcc aga ttt aag caa caa ctt caa gcc 1824

Tyr Lys Phe Ser Ser Val Ser Ser Arg Phe Lys Gln Gln Leu Gln Ala

595 600 605

ctc atg gaa act ctc agc aaa aca gag cct cac tat gtt cgg tgt gtg 1872

Leu Met Glu Thr Leu Ser Lys Thr Glu Pro His Tyr Val Arg Cys Val

610 615 620

aag cca aac tca ctc aac aga cct caa aag ttt gag agt ctt agt gtt 1920

Lys Pro Asn Ser Leu Asn Arg Pro Gln Lys Phe Glu Ser Leu Ser Val

625 630 635 640

tta cat caa ctt cgt tgt ggg ggt gta ctg gaa gct gtt cgg att agt 1968

Leu His Gln Leu Arg Cys Gly Gly Val Leu Glu Ala Val Arg Ile Ser

645 650 655

cta gca ggg tat ccc act cga agg aat tat tca gac ttc gtg gat cgt 2016

Leu Ala Gly Tyr Pro Thr Arg Arg Asn Tyr Ser Asp Phe Val Asp Arg

660 665 670

ttt ggt ctg cta gct cca gaa ttc atg gat gag agc aat gat gag cag 2064

Phe Gly Leu Leu Ala Pro Glu Phe Met Asp Glu Ser Asn Asp Glu Gln

675 680 685

gca ctg act gag aaa atc ttg agt aaa tta ggt ctt ggg aat tat cag 2112

Ala Leu Thr Glu Lys Ile Leu Ser Lys Leu Gly Leu Gly Asn Tyr Gln

690 695 700

cta gga agg aca aaa gtg ttc cta aga gct ggt caa att ggc att ttg 2160

Leu Gly Arg Thr Lys Val Phe Leu Arg Ala Gly Gln Ile Gly Ile Leu

705 710 715 720

gac tct agg cgg gct gaa gtc ctt gat gct tct gca aga ctt att cag 2208

Asp Ser Arg Arg Ala Glu Val Leu Asp Ala Ser Ala Arg Leu Ile Gln

725 730 735

cga aga ctg aga aca ttt gta acg cat cag aac ttc atc tct gca cgg 2256

Arg Arg Leu Arg Thr Phe ValThr His Gln Asn Phe Ile Ser Ala Arg

740 745 750

gct tct gca att tca att cag gca tac tgt aga gga tgc ctg tct cga 2304

Ala Ser Ala Ile Ser Ile Gln Ala Tyr Cys Arg Gly Cys Leu Ser Arg

755 760 765

aat gct tat gcc acc aga agg aat gcg gcg gca gct gtc ttg gtc caa 2352

Asn Ala Tyr Ala Thr Arg Arg Asn Ala Ala Ala Ala Val Leu Val Gln

770 775 780

aag cat gtg cgc agg tgg ctg tca aga tgt gca ttt gta aaa ctt gta 2400

Lys His Val Arg Arg Trp Leu Ser Arg Cys Ala Phe Val Lys Leu Val

785 790 795 800

tca gct gcc att gta tta cag tct tgc atc cgt gct gac tca act cgc 2448

Ser Ala Ala Ile Val Leu Gln Ser Cys Ile Arg Ala Asp Ser Thr Arg

805 810 815

tta aag ttt tca cat cag aaa gag cat cga gct gct tct cta att cag 2496

Leu Lys Phe Ser His Gln Lys Glu His Arg Ala Ala Ser Leu Ile Gln

820 825 830

gct cat tgg aga atc cat aag ttt cgc tca gca ttc agg cac cgt cag 2544

Ala His Trp Arg Ile His Lys Phe Arg Ser Ala Phe Arg His Arg Gln

835 840 845

tca tct att att gct att cag tgt cgt tgg cga cag aag ctt gcg aag 2592

Ser Ser Ile Ile Ala Ile Gln Cys Arg Trp Arg Gln Lys Leu Ala Lys

850 855 860

aga gag ttt aga aaa ctt aaa cag gtt gct aat gaa gca ggt gct ttg 2640

Arg Glu Phe Arg Lys Leu Lys Gln Val Ala Asn Glu Ala Gly Ala Leu

865 870 875 880

cga tta gct aaa acg aaa ctt gaa aaa cgg tta gaa gat ctt gaa tgg 2688

Arg Leu Ala Lys Thr Lys Leu Glu Lys Arg Leu Glu Asp Leu Glu Trp

885 890 895

cgg ttg cag ctt gag aaa cga ttg aga aca agt ggt gaa gag gcc aag 2736

Arg Leu Gln Leu Glu Lys Arg Leu Arg Thr Ser Gly Glu Glu Ala Lys

900 905 910

tca agt gaa ata tcc aag ctt cag aaa aca ttg gaa tcc ttc agc ctc 2784

Ser Ser Glu Ile Ser Lys Leu Gln Lys Thr Leu Glu Ser Phe Ser Leu

915 920 925

aaa cta gac gca gct agg ctg gct acc att aat gag tgc aat aaa aat 2832

Lys Leu Asp Ala Ala Arg Leu Ala Thr Ile Asn Glu Cys Asn Lys Asn

930 935 940

gcg gta ctt gaa aag caa cta gac ata tcc atg aag gag aag tct gct 2880

Ala Val Leu Glu Lys Gln Leu Asp Ile Ser Met Lys Glu Lys Ser Ala

945 950 955 960

gtt gaa aga gag ctt aat gga atg gtt gaa cta aaa aaa gat aac gcc 2928

Val Glu Arg Glu Leu Asn Gly Met Val Glu Leu Lys Lys Asp Asn Ala

965 970 975

ttg ctg aag aat tcg atg aac tcc ttg gaa aag aag aat cgg gtt ctt 2976

Leu Leu Lys Asn Ser Met Asn Ser Leu Glu Lys Lys Asn Arg Val Leu

980 985 990

gag aag gag ctt ctc aat gct aaa acc aat tgc aat aat aca cta cag 3024

Glu Lys Glu Leu Leu Asn Ala Lys Thr Asn Cys Asn Asn Thr Leu Gln

995 1000 1005

aag ttg aag gaa gct gaa aaa agg tgt tct gaa ctc cag acg agt 3069

Lys Leu Lys Glu Ala Glu Lys Arg Cys Ser Glu Leu Gln Thr Ser

1010 1015 1020

gtt caa agt ctt gag gag aaa ctc tct cat ctg gaa aac gag aac 3114

Val Gln Ser Leu Glu Glu Lys Leu Ser His Leu Glu Asn Glu Asn

1025 1030 1035

cag gtc ttg atg caa aag acg cta att aca tcc cca gag aga ata 3159

Gln Val Leu Met Gln Lys Thr Leu Ile Thr Ser Pro Glu Arg Ile

1040 1045 1050

gga cag ata ctt ggt gaa aaa cac tct agt gct gtt gta cca gcc 3204

Gly Gln Ile Leu Gly Glu Lys His Ser Ser Ala Val Val Pro Ala

1055 1060 1065

caa aat gac agg aga tct gta ttt gag aac tac gaa ttg ctc tcc 3249

Gln Asn Asp Arg Arg Ser Val Phe Glu Asn Tyr Glu Leu Leu Ser

1070 1075 1080

agg tgt ata aag gaa aat ttg gga ttc aat gat gat aag cca ctg 3294

Arg Cys Ile Lys Glu Asn Leu Gly Phe Asn Asp Asp Lys Pro Leu

1085 1090 1095

gct gcc tgt gta ata tac aaa tgt ctt ctg cac tgg cgt gcc ttt 3339

Ala Ala Cys Val Ile Tyr Lys Cys Leu Leu His Trp Arg Ala Phe

1100 1105 1110

gaa tct gag agc aca gcc ata ttt aac atc att att gag gga atc 3384

Glu Ser Glu Ser Thr Ala Ile Phe Asn Ile Ile Ile Glu Gly Ile

1115 1120 1125

aat gaa gcc ctg aag aga aat ctg cgg tca aat agt ttt cta aat 3429

Asn Glu Ala Leu Lys Arg Asn Leu Arg Ser Asn Ser Phe Leu Asn

1130 1135 1140

gca agt gct cag cgt tct ggg agg gct gca tat gga gta aag tct 3474

Ala Ser Ala Gln Arg Ser Gly Arg Ala Ala Tyr Gly Val Lys Ser

1145 1150 1155

cct ttt aaa ctt cat gga cct gat gat ggt gct tcg cat ata gaa 3519

Pro Phe Lys Leu His Gly Pro Asp Asp Gly Ala Ser His Ile Glu

1160 1165 1170

gca aga tat cca gca tta tta ttt aaa cag cag ctg aca gca tgt 3564

Ala Arg Tyr Pro Ala Leu Leu Phe Lys Gln Gln Leu Thr Ala Cys

1175 1180 1185

gtg gag aag att tat ggt tta att cgt gat aat ttg aaa aaa gaa 3609

Val Glu Lys Ile Tyr Gly Leu Ile Arg Asp Asn Leu Lys Lys Glu

1190 1195 1200

tta tca ccg ctt ctg gga tca tgc att cag gta ccc tcg ttc ttc 3654

Leu Ser Pro Leu Leu Gly Ser Cys Ile Gln Val Pro Ser Phe Phe

1205 1210 1215

att cgc aaa ctt gtg act cag gtt ttc tca ttc atc aac cta tca 3699

Ile Arg Lys Leu Val Thr Gln Val Phe Ser Phe Ile Asn Leu Ser

1220 1225 1230

ctt ttc aac agt ctt ctt ctt cgt cgt gaa tgt tgc aca ttt tca 3744

Leu Phe Asn Ser Leu Leu Leu Arg Arg Glu Cys Cys Thr Phe Ser

1235 1240 1245

aat ggg gaa tat gtg aaa tct ggg att tca gaa ttg gag aag tgg 3789

Asn Gly Glu Tyr Val Lys Ser Gly Ile Ser Glu Leu Glu Lys Trp

1250 1255 1260

ata gct aat gcg aag gag gag gta ttg act ata agg caa ata tat 3834

Ile Ala Asn Ala Lys Glu Glu Val Leu Thr Ile Arg Gln Ile Tyr

1265 1270 1275

cga ata agt acg atg tac tgg gat gat aaa tat gga act caa agt 3879

Arg Ile Ser Thr Met Tyr Trp Asp Asp Lys Tyr Gly Thr Gln Ser

1280 1285 1290

gtc tca agt gag gtg gtt tct caa atg agg gta ctt gtg gac aag 3924

Val Ser Ser Glu Val Val Ser Gln Met Arg Val Leu Val Asp Lys

1295 1300 1305

gat aac caa aaa caa aca tca aat tcg ttc ttg ctg gac gat gat 3969

Asp Asn Gln Lys Gln Thr Ser Asn Ser Phe Leu Leu Asp Asp Asp

1310 1315 1320

atg agc att cct ttc tct gca gaa gat ata gac aag gct att cca 4014

Met Ser Ile Pro Phe Ser Ala Glu Asp Ile Asp Lys Ala Ile Pro

1325 1330 1335

gta tta gac cca tca gaa ata gaa cct cca aaa ttc gta tca gaa 4059

Val Leu Asp Pro Ser Glu Ile Glu Pro Pro Lys Phe Val Ser Glu

1340 1345 1350

tat act tgt gca cag tcc ctt gtg aag aaa ccc tcc ata gct tca 4104

Tyr Thr Cys Ala Gln Ser Leu Val Lys Lys Pro Ser Ile Ala Ser

1355 1360 1365

acc tca aag cag atc att tga 4125

Thr Ser Lys Gln Ile Ile

1370

<210>78

<211>1374

<212>PRT

＜213〉Arabidopis thaliana

<400>78

Met Arg Asn Cys Leu Pro Met Glu Leu Asn Leu Arg Lys Gly Asp Lys

1 5 10 15

Val Trp Val Glu Asp Lys Asp Leu Ala Trp Ile Ala Ala Asp Val Leu

20 25 30

Asp Ser Phe Asp Asn Lys Leu His Val Glu Thr Ser Thr Gly Lys Lys

35 40 45

Leu Phe Arg Arg Asp Pro Asp Asp Glu Glu His Asn Gly Val Asp Asp

50 55 60

Met Thr Lys Leu Thr Tyr Leu His Glu Ala Gly Val Leu Tyr Asn Leu

65 70 75 80

Gln Arg Arg Tyr Ala Leu Asn Asp Ile Tyr Thr Tyr Thr Gly Ser Ile

85 90 95

Leu Ile Ala Val Asn Pro Phe Lys Lys Leu Pro His Leu Tyr Asn Gly

100 105 110

His Met Met Glu Gln Tyr Met Gly Ala Pro Phe Gly Glu Leu Ser Pro

115 120 125

His Val Phe Ala Val Ser Asp Val Ala Tyr Arg Ala Met Ile Asp Asp

130 135 140

Ser Arg Ser Gln Ser Ile Leu Val Ser Gly Glu Ser Gly Ala Gly Lys

145 150 155 160

Thr Glu Thr Thr Lys Leu Ile Met Gln Tyr Leu Thr Phe Val Gly Gly

165 170 175

Arg Ala Thr Asp Asp Asp Arg Ser Val Glu Gln Gln Val Leu Glu Ser

180 185 190

Asn Pro Leu Leu Glu Ala Phe Gly Asn Ala Lys Thr Val Arg Asn Asp

195 200 205

Asn Ser Ser Arg Phe Gly Lys Phe Val Glu Ile Gln Phe Asp Thr Asn

210 215 220

Gly Arg Ile Ser Gly Ala Ala Ile Arg Thr Tyr Leu Leu Glu Arg Ser

225 230 235 240

Arg Val Val Arg Ile Thr Asp Pro Glu Arg Asn Tyr His Cys Phe Tyr

245 250 255

Gln Leu Cys Ala Ser Gly Asn Asp Ala Glu Lys Tyr Lys Leu Ser Asn

260 265 270

Pro Arg Gln Phe His Tyr Leu Asn Gln Ser Lys Thr Tyr Glu Leu Glu

275 280 285

Gly Val Ser Ser Ala Glu Glu Tyr Lys Asn Thr Arg Arg Ala Met Asp

290 295 300

Ile Val Gly Ile Ser Gln Asp Glu Gln Glu Gly Ile Phe Arg Thr Leu

305 310 315 320

Ala Ala Ile Leu His Leu Gly Asn Val Glu Phe Ser Ser Gly Arg Glu

325 330 335

His Asp Ser Ser Val Val Lys Asp Pro Glu Ser Arg His His Leu Gln

340 345 350

Met Ala Ala Asp Leu Phe Lys Cys Asp Ala Asn Leu Leu Leu Ala Ser

355 360 365

Leu Cys Thr Arg Ser Ile Leu Thr Arg Glu Gly Ile Ile Ile Lys Ala

370 375 380

Leu Asp Pro Asn Ala Ala Val Thr Ser Arg Asp Thr Leu Ala Lys Thr

385 390 395 400

Val Tyr Ala His Leu Phe Asp Trp Leu Val Asp Lys Ile Asn Lys Ser

405 410 415

Val Gly Gln Asp Pro Glu Ser Arg Phe Gln Ile Gly Val Leu Asp Ile

420 425 430

Tyr Gly Phe Glu Cys Phe Lys Asn Asn Ser Phe Glu Gln Phe Cys Ile

435 440 445

Asn Phe Ala Asn Glu Lys Leu Gln Gln His Phe Asn Glu His Val Phe

450 455 460

Lys Met Glu Gln Asp Glu Tyr Arg Lys Glu Glu Ile Asn Trp Ser Tyr

465 470 475 480

Ile Glu Phe Ile Asp Asn Gln Asp Val Leu Asp Leu Ile Glu Lys Lys

485 490 495

Pro Ile Gly Val Ile Ala Leu Leu Asp Glu Ala Cys Met Phe Pro Arg

500 505 510

Ser Thr His Glu Ser Phe Ser Met Lys Leu Phe Gln Asn Phe Arg Phe

515 520 525

His Pro Arg Leu Glu Lys Pro Lys Phe Ser Glu Thr Asp Phe Thr Leu

530 535 540

Ser His Tyr Ala Gly Lys Ala Thr Phe Leu Asp Lys Asn Arg Asp Tyr

545 550 555 560

Thr Ile Val Glu His Cys Asn Leu Leu Ser Ser Ser Lys Cys Pro Phe

565 570 575

Val Ala Gly Ile Phe Pro Ser Ala Pro Glu Glu Ser Thr Arg Ser Ser

580 585 590

Tyr Lys Phe Ser Ser Val Ser Ser Arg Phe Lys Gln Gln Leu Gln Ala

595 600 605

Leu Met Glu Thr Leu Ser Lys Thr Glu Pro His Tyr Val Arg Cys Val

610 615 620

Lys Pro Asn Ser Leu Asn Arg Pro Gln Lys Phe Glu Ser Leu Ser Val

625 630 635 640

Leu His Gln Leu Arg Cys Gly Gly Val Leu Glu Ala Val Arg Ile Ser

645 650 655

Leu Ala Gly Tyr Pro Thr Arg Arg Asn Tyr Ser Asp Phe Val Asp Arg

660 665 670

Phe Gly Leu Leu Ala Pro Glu Phe Met Asp Glu Ser Asn Asp Glu Gln

675 680 685

Ala Leu Thr Glu Lys Ile Leu Ser Lys Leu Gly Leu Gly Asn Tyr Gln

690 695 700

Leu Gly Arg Thr Lys Val Phe Leu Arg Ala Gly Gln Ile Gly Ile Leu

705 710 715 720

Asp Ser Arg Arg Ala Glu Val Leu Asp Ala Ser Ala Arg Leu Ile Gln

725 730 735

Arg Arg Leu Arg Thr Phe Val Thr His Gln Asn Phe Ile Ser Ala Arg

740 745 750

Ala Ser Ala Ile Ser Ile Gln Ala Tyr Cys Arg Gly Cys Leu Ser Arg

755 760 765

Asn Ala Tyr Ala Thr Arg Arg Asn Ala Ala Ala Ala Val Leu Val Gln

770 775 780

Lys His Val Arg Arg Trp Leu Ser Arg Cys Ala Phe ValLys Leu Val

785 790 795 800

Ser Ala Ala Ile Val Leu Gln Ser Cys Ile Arg Ala Asp Ser Thr Arg

805 810 815

Leu Lys Phe Ser His Gln Lys Glu His Arg Ala Ala Ser Leu Ile Gln

820 825 830

Ala His Trp Arg Ile His Lys Phe Arg Ser Ala Phe Arg His Arg Gln

835 840 845

Ser Ser Ile Ile Ala Ile Gln Cys Arg Trp Arg Gln Lys Leu Ala Lys

850 855 860

Arg Glu Phe Arg Lys Leu Lys Gln Val Ala Asn Glu Ala Gly Ala Leu

865 870 875 880

Arg Leu Ala Lys Thr Lys Leu Glu Lys Arg Leu Glu Asp Leu Glu Trp

885 890 895

Arg Leu Gln Leu Glu Lys Arg Leu Arg Thr Ser Gly Glu Glu Ala Lys

900 905 910

Ser Ser Glu Ile Ser Lys Leu Gln Lys Thr Leu Glu Ser Phe Ser Leu

915 920 925

Lys Leu Asp Ala Ala Arg Leu Ala Thr Ile Asn Glu Cys Asn Lys Asn

930 935 940

Ala Val Leu Glu Lys Gln Leu Asp Ile Ser Met Lys Glu Lys Ser Ala

945 950 955 960

Val Glu Arg Glu Leu Asn Gly Met Val Glu Leu Lys Lys Asp Asn Ala

965 970 975

Leu Leu Lys Asn Ser Met Asn Ser Leu Glu Lys Lys Asn Arg Val Leu

980 985 990

Glu Lys Glu Leu Leu Asn Ala Lys Thr Asn Cys Asn Asn Thr Leu Gln

995 1000 1005

Lys Leu Lys Glu Ala Glu Lys Arg Cys Ser Glu Leu Gln Thr Ser

1010 1015 1020

Val Gln Ser Leu Glu Glu Lys Leu Ser His Leu Glu Asn Glu Asn

1025 1030 1035

Gln Val Leu Met Gln Lys Thr Leu Ile Thr Ser Pro Glu Arg Ile

1040 1045 1050

Gly Gln Ile Leu Gly Glu Lys His Ser Ser Ala Val Val Pro Ala

1055 1060 1065

Gln Asn Asp Arg Arg Ser Val Phe Glu Asn Tyr Glu Leu Leu Ser

1070 1075 1080

Arg Cys Ile Lys Glu Asn Leu Gly Phe Asn Asp Asp Lys Pro Leu

1085 1090 1095

Ala Ala Cys Val Ile Tyr Lys Cys Leu Leu His Trp Arg Ala Phe

1100 1105 1110

Glu Ser Glu Ser Thr Ala Ile Phe Asn Ile Ile Ile Glu Gly Ile

1115 1120 1125

Asn Glu Ala Leu Lys Arg Asn Leu Arg Ser Asn Ser Phe Leu Asn

1130 1135 1140

Ala Ser Ala Gln Arg Ser Gly Arg Ala Ala Tyr Gly Val Lys Ser

1145 1150 1155

Pro Phe Lys Leu His Gly Pro Asp Asp Gly Ala Ser His Ile Glu

1160 1165 1170

Ala Arg Tyr Pro Ala Leu Leu Phe Lys Gln Gln Leu Thr Ala Cys

1175 1180 1185

Val Glu Lys Ile Tyr Gly Leu Ile Arg Asp Asn Leu Lys Lys Glu

1190 1195 1200

Leu Ser Pro Leu Leu Gly Ser Cys Ile Gln Val Pro Ser Phe Phe

1205 1210 1215

Ile Arg Lys Leu Val Thr Gln Val Phe Ser Phe Ile Asn Leu Ser

1220 1225 1230

Leu Phe Asn Ser Leu Leu Leu Arg Arg Glu Cys Cys Thr Phe Ser

1235 1240 1245

Asn Gly Glu Tyr Val Lys Ser Gly Ile Ser Glu Leu Glu Lys Trp

1250 1255 1260

Ile Ala Asn Ala Lys Glu Glu Val Leu Thr Ile Arg Gln Ile Tyr

1265 1270 1275

Arg Ile Ser Thr Met Tyr Trp Asp Asp Lys Tyr Gly Thr Gln Ser

1280 1285 1290

Val Ser Ser Glu Val Val Ser Gln Met Arg Val Leu Val Asp Lys

1295 1300 1305

Asp Asn Gln Lys Gln Thr Ser Asn Ser Phe Leu Leu Asp Asp Asp

1310 1315 1320

Met Ser Ile Pro Phe Ser Ala Glu Asp Ile Asp Lys Ala Ile Pro

1325 1330 1335

Val Leu Asp Pro Ser Glu Ile Glu Pro Pro Lys Phe Val Ser Glu

1340 1345 1350

Tyr Thr Cys Ala Gln Ser Leu Val Lys Lys Pro Ser Ile Ala Ser

1355 1360 1365

Thr Ser Lys Gln Ile lle

1370

<210>79

<211>1392

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(1392)

<400>79

atg gat ttc tcc ggt ttg ttt ctc act ctc tcc gcg gcg gct ctg ttt 48

Met Asp Phe Ser Gly Leu Phe Leu Thr Leu Ser Ala Ala Ala Leu Phe

1 5 10 15

ctc tgt tta ctc cga ttt atc gcc gga gtc cgc cgt agc tcc tcc acg 96

Leu Cys Leu Leu Arg Phe Ile Ala Gly Val Arg Arg Ser Ser Ser Thr

20 25 30

aaa ctc cct ctt cct ccg gga aca atg ggt tat cct tac gtc ggc gaa 144

Lys Leu Pro Leu Pro Pro Gly Thr Met Gly Tyr Pro Tyr Val Gly Glu

35 40 45

aca ttc caa ctt tac tca caa gac cct aat gtg ttc ttt gca gca aaa 192

Thr Phe Gln Leu Tyr Ser Gln Asp Pro Asn Val Phe Phe Ala Ala Lys

50 55 60

cag aga aga tac gga tcg gtg ttc aag act cat gta ttg gga tgt cca 240

Gln Arg Arg Tyr Gly Ser Val Phe Lys Thr His Val Leu Gly Cys Pro

65 70 75 80

tgt gtg atg atc tcg agc cct gaa gca gcg aaa ttc gta ttg gtt aca 288

Cys Val Met Ile Ser Ser Pro Glu Ala Ala Lys Phe Val Leu Val Thr

85 90 95

aag tct cat ttg ttt aaa ccg act ttt ccg gcc agt aaa gag agg atg 336

Lys Ser His Leu Phe Lys Pro Thr Phe Pro Ala Ser Lys Glu Arg Met

100 105 110

ctt gga aaa caa gcc atc ttc ttc cat caa gga gat tat cat tcc aaa 384

Leu Gly Lys Gln Ala Ile Phe Phe His Gln Gly Asp Tyr His Ser Lys

115 120 125

ctt aga aag ctt gtt tta aga gct ttc atg cct gat gca atc aga aac 432

Leu Arg Lys Leu Val Leu Arg Ala Phe Met Pro Asp Ala Ile Arg Asn

130 135 140

atg gtc cct cac att gaa tca att gct caa gaa tca ctc aat tct tgg 480

Met Val Pro His Ile Glu Ser Ile Ala Gln Glu Ser Leu Asn Ser Trp

145 150 155 160

gat gga act caa ctc aac act tac cag gaa atg aaa aca tac act ttc 528

Asp Gly Thr Gln Leu Asn Thr Tyr Gln Glu Met Lys Thr Tyr Thr Phe

165 170 175

aat gtt gcg tta atc tca ata ctc ggc aaa gac gaa gtt tat tac cga 576

Asn Val Ala Leu Ile Ser Ile Leu Gly Lys Asp Glu Val Tyr Tyr Arg

180 185 190

gaa gat cta aaa cga tgc tac tac att cta gag aaa ggt tac aat tcg 624

Glu Asp Leu Lys Arg Cys Tyr Tyr Ile Leu Glu Lys Gly Tyr Asn Ser

195 200 205

atg ccg att aat ctt cca gga aca tta ttc cac aaa gcc atg aaa gct 672

Met Pro Ile Asn Leu Pro Gly Thr Leu Phe His Lys Ala Met Lys Ala

210 215 220

cgc aag gag cta gct caa atc ctc gct aac atc tta tcc aaa aga aga 720

Arg Lys Glu Leu Ala Gln Ile Leu Ala Asn Ile Leu Ser Lys Arg Arg

225 230 235 240

caa aac cca tca tca cac aca gat ctc ctc gga tca ttc atg gaa gac 768

Gln Asn Pro Ser Ser His Thr Asp Leu Leu Gly Ser Phe Met Glu Asp

245 250 255

aaa gca gga tta acc gac gaa caa atc gcc gat aac atc atc gga gta 816

Lys Ala Gly Leu Thr Asp Glu Gln Ile Ala Asp Asn Ile Ile Gly Val

260 265 270

atc ttc gcc gca aga gac acg acg gcg agt gtt ctg acg tgg atc ctc 864

Ile Phe Ala Ala Arg Asp Thr Thr Ala Ser Val Leu Thr Trp Ile Leu

275 280 285

aag tac tta gct gat aat cca act gtt cta gaa gct gtc act gaa gag 912

Lys Tyr Leu Ala Asp Asn Pro Thr Val Leu Glu Ala Val Thr Glu Glu

290 295 300

caa atg gca ata agg aaa gat aaa aaa gaa gga gag agt ctc act tgg 960

Gln Met Ala Ile Arg Lys Asp Lys Lys Glu Gly Glu Ser Leu Thr Trp

305 310 315 320

gaa gat aca aag aag atg cca tta act tat aga gta atc caa gag aca 1008

Glu Asp Thr Lys Lys Met Pro Leu Thr Tyr Arg Val Ile Gln Glu Thr

325 330 335

tta aga gct gct aca atc tta tct ttc aca ttt aga gaa gct gtc gaa 1056

Leu Arg Ala Ala Thr Ile Leu Ser Phe Thr Phe Arg Glu Ala Val Glu

340 345 350

gat gtc gaa tac gaa gga tat ttg ata cca aag gga tgg aaa gta ctg 1104

Asp Val Glu Tyr Glu Gly Tyr Leu Ile Pro Lys Gly Trp Lys Val Leu

355 360 365

cca cta ttc aga aat att cat cac aat gct gat ata ttt tcg gat ccg 1152

Pro Leu Phe Arg Asn Ile His His Asn Ala Asp Ile Phe Ser Asp Pro

370 375 380

ggg aaa ttc gat ccg tcg aga ttc gaa gtt gcg ccg aaa ccg aat aca 1200

Gly Lys Phe Asp Pro Ser Arg Phe Glu Val Ala Pro Lys Pro Asn Thr

385 390 395 400

ttc atg cct ttt ggt agt ggg att cat tct tgt cca ggc aat gag tta 1248

Phe Met Pro Phe Gly Ser Gly Ile His Ser Cys Pro Gly Asn Glu Leu

405 410 415

gct aaa ctt gaa atc tct gtt cta atc cat cat ctc acc act aag tac 1296

Ala Lys Leu Glu Ile Ser Val Leu Ile His His Leu Thr Thr Lys Tyr

420 425 430

aga tgg tca atc gta ggg cct agc gat gga att cag tat ggg ccg ttc 1344

Arg Trp Ser Ile Val Gly Pro Ser Asp Gly Ile Gln Tyr Gly Pro Phe

435 440 445

gct ctt cct cag aat gga ttg cct att gcc ttg gaa cga aaa cca tag 1392

Ala Leu Pro Gln Asn Gly Leu Pro Ile Ala Leu Glu Arg Lys Pro

450 455 460

<210>80

<211>463

<212>PRT

＜213〉Arabidopis thaliana

<400>80

Met Asp Phe Ser Gly Leu Phe Leu Thr Leu Ser Ala Ala Ala Leu Phe

1 5 10 15

Leu Cys Leu Leu Arg Phe Ile Ala Gly Val Arg Arg Ser Ser Ser Thr

20 25 30

Lys Leu Pro Leu Pro Pro Gly Thr Met Gly Tyr Pro Tyr Val Gly Glu

35 40 45

Thr Phe Gln Leu Tyr Ser Gln Asp Pro Asn Val Phe Phe Ala Ala Lys

50 55 60

Gln Arg Arg Tyr Gly Ser Val Phe Lys Thr His Val Leu Gly Cys Pro

65 70 75 80

Cys Val Met Ile Ser Ser Pro Glu Ala Ala Lys Phe Val Leu Val Thr

85 90 95

Lys Ser His Leu Phe Lys Pro Thr Phe Pro Ala Ser Lys Glu Arg Met

100 105 110

Leu Gly Lys Gln Ala Ile Phe Phe His Gln Gly Asp Tyr His Ser Lys

115 120 125

Leu Arg Lys Leu Val Leu Arg Ala Phe Met Pro Asp Ala Ile Arg Asn

130 135 140

Met Val Pro His Ile Glu Ser Ile Ala Gln Glu Ser Leu Asn Ser Trp

145 150 155 160

Asp Gly Thr Gln Leu Asn Thr Tyr Gln Glu Met Lys Thr Tyr Thr Phe

165 170 175

Asn Val Ala Leu Ile Ser Ile Leu Gly Lys Asp Glu Val Tyr Tyr Arg

180 185 190

Glu Asp Leu Lys Arg Cys Tyr Tyr Ile Leu Glu Lys Gly Tyr Asn Ser

195 200 205

Met Pro Ile Asn Leu Pro Gly Thr Leu Phe His Lys Ala Met Lys Ala

210 215 220

Arg Lys Glu Leu Ala Gln Ile Leu Ala Asn Ile Leu Ser Lys Arg Arg

225 230 235 240

Gln Asn Pro Ser Ser His Thr Asp Leu Leu Gly Ser Phe Met Glu Asp

245 250 255

Lys Ala Gly Leu Thr Asp Glu Gln Ile Ala Asp Asn Ile Ile Gly Val

260 265 270

Ile Phe Ala Ala Arg Asp Thr Thr Ala Ser Val Leu Thr Trp Ile Leu

275 280 285

Lys Tyr Leu Ala Asp Asn Pro Thr Val Leu Glu Ala Val Thr Glu Glu

290 295 300

Gln Met Ala Ile Arg Lys Asp Lys Lys Glu Gly Glu Ser Leu Thr Trp

305 310 315 320

Glu Asp Thr Lys Lys Met Pro Leu Thr Tyr Arg Val Ile Gln Glu Thr

325 330 335

Leu Arg Ala Ala Thr Ile Leu Ser Phe Thr Phe Arg Glu Ala Val Glu

340 345 350

Asp Val Glu Tyr Glu Gly Tyr Leu Ile Pro Lys Gly Trp Lys Val Leu

355 360 365

Pro Leu Phe Arg Asn Ile His His Asn Ala Asp Ile Phe Ser Asp Pro

370 375 380

Gly Lys Phe Asp Pro Ser Arg Phe Glu Val Ala Pro Lys Pro Asn Thr

385 390 395 400

Phe Met Pro Phe Gly Ser Gly Ile His Ser Cys Pro Gly Asn Glu Leu

405 410 415

Ala Lys Leu Glu Ile Ser Val Leu Ile His His Leu Thr Thr Lys Tyr

420 425 430

Arg Trp Ser Ile Val Gly Pro Ser Asp Gly Ile Gln Tyr Gly Pro Phe

435 440 445

Ala Leu Pro Gln Asn Gly Leu Pro Ile Ala Leu Glu Arg Lys Pro

450 455 460

<210>81

<211>1452

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(1452)

<400>81

atg gcg gat ttg tta aga aaa gtg aaa tcg ata aag aag aag cag gat 48

Met Ala Asp Leu Leu Arg Lys Val Lys Ser Ile Lys Lys Lys Gln Asp

1 5 10 15

cag agc aat cat caa gct ctg atc ctt ggc aaa tac gaa atg ggt agg 96

Gln Ser Asn His Gln Ala Leu Ile Leu Gly Lys Tyr Glu Met Gly Arg

20 25 30

ctt ctt ggc cac gga acc ttc gct aaa gtc tat ctc gca cga aac gct 144

Leu Leu Gly His Gly Thr Phe Ala Lys Val Tyr Leu Ala Arg Asn Ala

35 40 45

caa tct gga gaa agc gta gcg atc aag gta att gac aaa gag aaa gtt 192

Gln Ser Gly Glu Ser Val Ala Ile Lys Val Ile Asp Lys Glu Lys Val

50 55 60

ctc aaa tcc ggt tta atc gca cac atc aaa cgc gag atc tcg atc ttg 240

Leu Lys Ser Gly Leu Ile Ala His Ile Lys Arg Glu Ile Ser Ile Leu

65 70 75 80

cgc cgt gtt cgt cat cct aac atc gtt cag cta ttc gaa gtc atg gcg 288

Arg Arg Val Arg His Pro Asn Ile Val Gln Leu Phe Glu Val Met Ala

85 90 95

acg aaa tct aag atc tat ttc gta atg gaa tat gtt aaa gga ggt gaa 336

Thr Lys Ser Lys Ile Tyr Phe Val Met Glu Tyr Val Lys Gly Gly Glu

100 105 ll0

ttg ttc aac aag gta gct aaa gga agg tta aaa gaa gaa atg gca cgt 384

Leu Phe Asn Lys Val Ala Lys Gly Arg Leu Lys Glu Glu Met Ala Arg

115 120 125

aaa tat ttt caa cag ttg atc tca gcc gta tcg ttt tgt cacttc cgt 432

Lys Tyr Phe Gln Gln Leu Ile Ser Ala Val Ser Phe Cys His Phe Arg

130 135 140

ggt gtt tat cat cga gat ttg aaa ccg gag aat ctt ctt tta gac gaa 480

Gly Val Tyr His Arg Asp Leu Lys Pro Glu Asn Leu Leu Leu Asp Glu

145 150 155 160

aat gga aac cta aaa gtc tct gat ttt ggt ctt agt gct gtt tct gat 528

Asn Gly Asn Leu Lys Val Ser Asp Phe Gly Leu Ser Ala Val Ser Asp

165 170 175

cag att cga caa gat ggg tta ttt cat act ttt tgt ggg acc cct gct 576

Gln Ile Arg Gln Asp Gly Leu Phe His Thr Phe Cys Gly Thr Pro Ala

180 185 190

tac gtg gca ccg gag gtt ctt gct cgg aaa ggc tac gat gga gct aaa 624

Tyr Val Ala Pro Glu Val Leu Ala Arg Lys Gly Tyr Asp Gly Ala Lys

195 200 205

gtc gat att tgg tct tgt gga gtg atc ttg ttt gtg tta atg gca ggg 672

Val Asp Ile Trp Ser Cys Gly Val Ile Leu Phe Val Leu Met Ala Gly

210 215 220

ttt ctt cct ttt cat gat cgg aat gtt atg gct atg tat aag aag att 720

Phe Leu Pro Phe His Asp Arg Asn Val Met Ala Met Tyr Lys Lys Ile

225 230 235 240

tac aga gga gat ttt agg tgt ccg aga tgg ttt ccg gtt gag att aac 768

Tyr Arg Gly Asp Phe Arg Cys Pro Arg Trp Phe Pro Val Glu Ile Asn

245 250 255

cgg tta ttg att cga atg ttg gag act aaa ccg gag aga cgg ttt aca 816

Arg Leu Leu Ile Arg Met Leu Glu Thr Lys Pro Glu Arg Arg Phe Thr

260 265 270

atg ccg gat att atg gag act agt tgg ttc aag aaa ggt ttt aag cat 864

Met Pro Asp Ile Met Glu Thr Ser Trp Phe Lys Lys Gly Phe Lys His

275 280 285

att aag ttt tat gtt gaa gat gat cat cag ctt tgt aac gtt gct gat 912

Ile Lys Phe Tyr Val Glu Asp Asp His Gln Leu Cys Asn Val Ala Asp

290 295 300

gat gat gag atc gaa tcg att gaa tcg gtt tcg ggg agg tct tct acg 960

Asp Asp Glu Ile Glu Ser Ile Glu Ser Val Ser Gly Arg Ser Ser Thr

305 310 315 320

gtt tct gaa ccg gaa gac ttc gag tct ttt gat ggg agg aga aga ggt 1008

Val Ser Glu Pro Glu Asp Phe Glu Ser Phe Asp Gly Arg Arg Arg Gly

325 330 335

ggt tcg atg cct aga ccg gca agt ttg aat gct ttc gat ctc att tcg 1056

Gly Ser Met Pro Arg Pro Ala Ser Leu Asn Ala Phe Asp Leu Ile Ser

340 345 350

ttt tcg cca gat ttt gat ctt tcg ggt ttg ttt gag gat gat ggt gaa 1104

Phe Ser Pro Gly Phe Asp Leu Ser Gly Leu Phe Glu Asp Asp Gly Glu

355 360 365

gga tct agg ttt gtg tct ggt gct cct gtt ggt cag atc att tct aag 1152

Gly Ser Arg Phe Val Ser Gly Ala Pro Val Gly Gln Ile Ile Ser Lys

370 375 380

ttg gag gaa atc gcg agg att gtg agt ttt act gtg cga aag aag gat 1200

Leu Glu Glu Ile Ala Arg Ile Val Ser Phe Thr Val Arg Lys Lys Asp

385 390 395 400

tgt aaa gtg agt ctt gaa ggt tca aga gaa gga agt atg aaa ggt cca 1248

Cys Lys Val Ser Leu Glu Gly Ser Arg Glu Gly Ser Met Lys Gly Pro

405 410 415

ttg tca att gct gct gag ata ttt gaa ctg aca cca gct ttg gtt gtt 1296

Leu Ser Ile Ala Ala Glu Ile Phe Glu Leu Thr Pro Ala Leu Val Val

420 425 430

gtt gaa gtg aag aag aaa gga ggt gat aaa atg gag tat gat gag ttt 1344

Val Glu Val Lys Lys Lys Gly Gly Asp Lys Met Glu Tyr Asp Glu Phe

435 440 445

tgt aat aag gag ttg aaa cct aag ttg cag aat ttg tct tcc gaa aat 1392

Cys Asn Lys Glu Leu Lys Pro Lys Leu Gln Asn Leu Ser Ser Glu Asn

450 455 460

ggc caa cgg gtt tct ggt tcg cgt tct ttg cca tcg ttt tta ctt tct 1440

Gly Gln Arg Val Ser Gly Ser Arg Ser Leu Pro Ser Phe Leu Leu Ser

465 470 475 480

gat act gat tag 1452

Asp Thr Asp

<210>82

<211>483

<212>PRT

＜213〉Arabidopis thaliana

<400>82

Met Ala Asp Leu Leu Arg Lys Val Lys Ser Ile Lys Lys Lys Gln Asp

1 5 10 15

Gln Ser Asn His Gln Ala Leu Ile Leu Gly Lys Tyr Glu Met Gly Arg

20 25 30

Leu Leu Gly His Gly Thr Phe Ala Lys Val Tyr Leu Ala Arg Asn Ala

35 40 45

Gln Ser Gly Glu Ser Val Ala Ile Lys Val Ile Asp Lys Glu Lys Val

50 55 60

Leu Lys Ser Gly Leu Ile Ala His Ile Lys Arg Glu Ile Ser Ile Leu

65 70 75 80

Arg Arg Val Arg His Pro Asn Ile Val Gln Leu Phe Glu Val Met Ala

85 90 95

Thr Lys Ser Lys Ile Tyr Phe Val Met Glu Tyr ValLys Gly Gly Glu

100 105 110

Leu Phe Asn Lys Val Ala Lys Gly Arg Leu Lys Glu Glu Met Ala Arg

115 120 125

Lys Tyr Phe Gln Gln Leu Ile Ser Ala Val Ser Phe Cys His Phe Arg

130 135 140

Gly Val Tyr His Arg Asp Leu Lys Pro Glu Asn Leu Leu Leu Asp Glu

145 150 155 160

Asn Gly Asn Leu Lys Val Ser Asp Phe Gly Leu Ser Ala Val Ser Asp

165 170 175

Gln Ile Arg Gln Asp Gly Leu Phe His Thr Phe Cys Gly Thr Pro Ala

180 185 190

Tyr Val Ala Pro Glu Val Leu Ala Arg Lys Gly Tyr Asp Gly Ala Lys

195 200 205

Val Asp Ile Trp Ser Cys Gly Val Ile Leu Phe Val Leu Met Ala Gly

210 215 220

Phe Leu Pro Phe His Asp Arg Asn Val Met Ala Met Tyr Lys Lys Ile

225 230 235 240

Tyr Arg Gly Asp Phe Arg Cys Pro Arg Trp Phe Pro Val Glu Ile Asn

245 250 255

Arg Leu Leu Ile Arg Met Leu Glu Thr Lys Pro Glu Arg Arg Phe Thr

260 265 270

Met Pro Asp Ile Met Glu Thr Ser Trp Phe Lys Lys Gly Phe Lys His

275 280 285

Ile Lys Phe Tyr Val Glu Asp Asp His Gln Leu Cys Asn Val Ala Asp

290 295 300

Asp Asp Glu Ile Glu Ser Ile Glu Ser Val Ser Gly Arg Ser Ser Thr

305 310 315 320

Val Ser Glu Pro Glu Asp Phe Glu Ser Phe Asp Gly Arg Arg Arg Gly

325 330 335

Gly Ser Met Pro Arg Pro Ala Ser Leu Asn Ala Phe Asp Leu Ile Ser

340 345 350

Phe Ser Pro Gly Phe Asp Leu Ser Gly Leu Phe Glu Asp Asp Gly Glu

355 360 365

Gly Ser Arg Phe Val Ser Gly Ala Pro Val Gly Gln Ile Ile Ser Lys

370 375 380

Leu Glu Glu Ile Ala Arg Ile Val Ser Phe Thr Val Arg Lys Lys Asp

385 390 395 400

Cys Lys Val Ser Leu Glu Gly Ser Arg Glu Gly Ser Met Lys Gly Pro

405 410 415

Leu Ser Ile Ala Ala Glu Ile Phe Glu Leu Thr Pro Ala Leu Val Val

420 425 430

Val Glu Val Lys Lys Lys Gly Gly Asp Lys Met Glu Tyr Asp Glu Phe

435 440 445

Cys Asn Lys Glu Leu Lys Pro Lys Leu Gln Asn Leu Ser Ser Glu Asn

450 455 460

Gly Gln Arg Val Ser Gly Ser Arg Ser Leu Pro Ser Phe Leu Leu Ser

465 470 475 480

Asp Thr Asp

<210>83

<211>1320

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(1320)

<400>83

atg gat aaa aac ggc ata gtt ttg atg cga aaa tat gaa tta ggt cgt 48

Met Asp Lys Asn Gly Ile Val Leu Met Arg Lys Tyr Glu Leu Gly Arg

1 5 10 15

ctt cta ggt caa ggc aca ttc gca aaa gtg tac cac gca cgc aac ata 96

Leu Leu Gly Gln Gly Thr Phe Ala Lys Val Tyr His Ala Arg Asn Ile

20 25 30

aaa aca gga gaa agc gta gcg atc aag gtg atc gac aaa cag aaa gtt 144

Lys Thr Gly Glu Ser Val Ala Ile Lys Val Ile Asp Lys Gln Lys Val

35 40 45

gcg aaa gtc gga tta atc gat caa atc aaa cga gaa ata tca gtg atg 192

Ala Lys Val Gly Leu Ile Asp Gln Ile Lys Arg Glu Ile Ser Val Met

50 55 60

cgt ctc gtt cgt cac ccc cac gtc gtc ttc ctc cat gaa gta atg gcg 240

Arg Leu Val Arg His Pro His ValVal Phe Leu His Glu Val Met Ala

65 70 75 80

agc aag aca aag atc tat ttc gct atg gaa tac gtt aaa ggc ggt gag 288

Ser Lys Thr Lys Ile Tyr Phe Ala Met Glu Tyr Val Lys Gly Gly Glu

85 90 95

ctt ttt gat aaa gtc tct aaa gga aag ctt aaa gaa aac att gct cga 336

Leu Phe Asp Lys Val Ser Lys Gly Lys Leu Lys Glu Asn Ile Ala Arg

100 105 110

aaa tat ttc cag caa ttg atc gga gca atc gat tat tgc cat agc cgc 384

Lys Tyr Phe Gln Gln Leu Ile Gly Ala Ile Asp Tyr Cys His Ser Arg

115 120 125

gga gtt tac cac cgc gat ctc aaa ccg gag aat ctt ctt cta gac gaa 432

Gly Val Tyr His Arg Asp Leu Lys Pro Glu Asn Leu Leu Leu Asp Glu

130 135 140

aac ggc gat ttg aaa ata tcg gat ttt ggc ctt agc gcg ttg agg gag 480

Asn Gly Asp Leu Lys Ile Ser Asp Phe Gly Leu Ser Ala Leu Arg Glu

145 150 155 160

tcg aag cag caa gat ggc ttg ctt cac acg aca tgt gga aca cct gct 528

Ser Lys Gln Gln Asp Gly Leu Leu His Thr Thr Cys Gly Thr Pro Ala

165 170 175

tac gtg gca cct gaa gtg ata ggc aag aaa ggt tat gat gga gct aaa 576

Tyr Val Ala Pro Glu Val Ile Gly Lys Lys Gly Tyr Asp Gly Ala Lys

180 185 190

gcc gat gtt tgg tct tgc ggg gtt gtg ttg tac gtg cta ttg gct gga 624

Ala Asp Val Trp Ser Cys Gly Val Val Leu Tyr Val Leu Leu Ala Gly

195 200 205

ttt ctt ccg ttt cac gag caa aat ctt gtg gaa atg tat cgg aaa atc 672

Phe Leu Pro Phe His Glu Gln Asn Leu Val Glu Met Tyr Arg Lys Ile

210 215 220

acg aaa ggc gaa ttc aaa tgt ccg aat tgg ttt cct ccc gag gtc aag 720

Thr Lys Gly Glu Phe Lys Cys Pro Asn Trp Phe Pro Pro Glu Val Lys

225 230 235 240

aag ttg ttg tct cgg att ctt gac cct aac cct aat tca aga atc aag 768

Lys Leu Leu Ser Arg Ile Leu Asp Pro Asn Pro Asn Ser Arg Ile Lys

245 250 255

att gaa aaa atc atg gag aat tcc tgg ttt caa aag ggt ttc aag aag 816

Ile Glu Lys Ile Met Glu Asn Ser Trp Phe Gln Lys Gly Phe Lys Lys

260 265 270

atc gaa acg cct aaa tct ccc gaa agt cat cag atc gac tca ctg atc 864

Ile Glu Thr Pro Lys Ser Pro Glu Ser His Gln Ile Asp Ser Leu Ile

275 280 285

agc gat gtc cac gca gct ttt tcc gta aaa ccg atg tct tac aac gcg 912

Ser Asp Val His Ala Ala Phe Ser Val Lys Pro Met Ser Tyr Asn Ala

290 295 300

ttt gac ttg atc tct tcg ctg tct caa gga ttc gat ctc tcg ggt ttg 960

Phe Asp Leu Ile Ser Ser Leu Ser Gln Gly Phe Asp Leu Ser Gly Leu

305 310 315 320

ttt gag aaa gaa gag aga tca gaa tcg aag ttt aca acg aag aaa gat 1008

Phe Glu Lys Glu Glu Arg Ser Glu Ser Lys Phe Thr Thr Lys Lys Asp

325 330 335

gca aaa gag ata gtg tcg aaa ttc gag gag ata gca aca agt agt gag 1056

Ala Lys Glu Ile Val Ser Lys Phe Glu Glu Ile Ala Thr Ser Ser Glu

340 345 350

aga ttc aat ttg acg aag agc gat gta gga gtg aag atg gaa gat aag 1104

Arg Phe Asn Leu Thr Lys Ser Asp Val Gly Val Lys Met Glu Asp Lys

355 360 365

aga gaa gga aga aaa gga cat ctt gcg att gat gtt gag ata ttt gaa 1152

Arg Glu Gly Arg Lys Gly His Leu Ala Ile Asp Val Glu Ile Phe Glu

370 375 380

gtg aca aat agt ttt cat atg gtt gag ttt aag aaa agt gga ggt gat 1200

Val Thr Asn Ser Phe His Met Val Glu Phe Lys Lys Ser Gly Gly Asp

385 390 395 400

aca atg gag tat aag caa ttt tgt gat cgt gag ctt agg cct tct ttg 1248

Thr Met Glu Tyr Lys Gln Phe Cys Asp Arg Glu Leu Arg Pro Ser Leu

405 410 415

aaa gat att gtt tgg aaa tgg caa gga aac aac aac aat agc aac aat 1296

Lys Asp Ile Val Trp Lys Trp Gln Gly Asn Asn Asn Asn Ser Asn Asn

420 425 430

gag aag att gaa gtg ata cat taa 1320

Glu Lys Ile Glu Val Ile His

435

<210>84

<211>439

<212>PRT

＜213〉Arabidopis thaliana

<400>84

Met Asp Lys Asn Gly Ile Val Leu Met Arg Lys Tyr Glu Leu Gly Arg

1 5 10 15

Leu Leu Gly Gln Gly Thr Phe Ala Lys Val Tyr His Ala Arg Asn Ile

20 25 30

Lys Thr Gly Glu Ser Val Ala Ile Lys Val Ile Asp Lys Gln Lys Val

35 40 45

Ala Lys Val Gly Leu Ile Asp Gln Ile Lys Arg Glu Ile Ser Val Met

50 55 60

Arg Leu Val Arg His Pro His Val ValPhe Leu His Glu Val Met Ala

65 70 75 80

Ser Lys Thr Lys Ile Tyr Phe Ala Met Glu Tyr Val Lys Gly Gly Glu

85 90 95

Leu Phe Asp Lys Val Ser Lys Gly Lys Leu Lys Glu Asn Ile Ala Arg

100 105 110

Lys Tyr Phe Gln Gln Leu Ile Gly Ala Ile Asp Tyr Cys His Ser Arg

115 120 125

Gly Val Tyr His Arg Asp Leu Lys Pro Glu Asn Leu Leu Leu Asp Glu

130 135 140

Asn Gly Asp Leu Lys Ile Ser Asp Phe Gly Leu Ser Ala Leu Arg Glu

145 150 155 160

Ser Lys Gln Gln Asp Gly Leu Leu His Thr Thr Cys Gly Thr Pro Ala

165 170 175

Tyr Val Ala Pro Glu Val Ile Gly Lys Lys Gly Tyr Asp Gly Ala Lys

180 185 190

Ala Asp Val Trp Ser Cys Gly Val Val Leu Tyr Val Leu Leu Ala Gly

195 200 205

Phe Leu Pro Phe His Glu Gln Asn Leu Val alu Met Tyr Arg Lys Ile

210 215 220

Thr Lys Gly Glu Phe Lys Cys Pro Asn Trp Phe Pro Pro Glu Val Lys

225 230 235 240

Lys Leu Leu Ser Arg Ile Leu Asp Pro Asn Pro Asn Ser Arg Ile Lys

245 250 255

Ile Glu Lys Ile Met Glu Asn Ser Trp Phe Gln Lys Gly Phe Lys Lys

260 265 270

Ile Glu Thr Pro Lys Ser Pro Glu Ser His Gln Ile Asp Ser Leu Ile

275 280 285

Ser Asp Val His Ala Ala Phe Ser Val Lys Pro Met Ser Tyr Asn Ala

290 295 300

Phe Asp Leu Ile Ser Ser Leu Ser Gln Gly Phe Asp Leu Ser Gly Leu

305 310 315 320

Phe Glu Lys Glu Glu Arg Ser Glu Ser Lys Phe Thr Thr Lys Lys Asp

325 330 335

Ala Lys Glu Ile Val Ser Lys Phe Glu Glu Ile Ala Thr Ser Ser Glu

340 345 350

Arg Phe Asn Leu Thr Lys Ser Asp Val Gly Val Lys Met Glu Asp Lys

355 360 365

Arg Glu Gly Arg Lys Gly His Leu Ala Ile Asp Val Glu Ile Phe Glu

370 375 380

Val Thr Asn Ser Phe His Met Val Glu Phe Lys Lys Ser Gly Gly Asp

385 390 395 400

Thr Met Glu Tyr Lys Gln Phe Cys Asp Arg Glu Leu Arg Pro Ser Leu

405 410 415

Lys Asp Ile Val Trp Lys Trp Gln Gly Asn Asn Asn Asn Ser Asn Asn

420 425 430

Glu Lys Ile Glu Val Ile His

435

<210>85

<211>963

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(963)

<400>85

atg tca aac cca ttg aaa gag tca aga gag gat att gca aat tct act 48

Met Ser Asn Pro Leu Lys Glu Ser Arg Glu Asp Ile Ala Asn Ser Thr

1 5 10 15

cct cac atg agg gat aat gag tat gtt cgg cta gtt gtg gct cat gaa 96

Pro His Met Arg Asp Asn Glu Tyr Val Arg Leu Val Val Ala His Glu

20 25 30

gcc tcc cca gct gaa acc gtg ttg tct cta tcg caa tca gag gtg cag 144

Ala Ser Pro Ala Glu Thr Val Leu Ser Leu Ser Gln Ser Glu Val Gln

35 40 45

agt aag aaa ttt atg tgg tgg tta aaa gct ttg gga ata tgt gca gtt 192

Ser Lys Lys Phe Met Trp Trp Leu Lys Ala Leu Gly Ile Cys Ala Val

50 55 60

gct ctc ttg ctt acg ctt gtt ttc gga aaa tgg gga gtt ccg ttt gtg 240

Ala Leu Leu Leu Thr Leu Val Phe Gly Lys Trp Gly Val Pro Phe Val

65 70 75 80

ttt caa aag gtt ctt att cca att ttg caa tgg gaa gca act gcg ttt 288

Phe Gln Lys Val Leu Ile Pro Ile Leu Gln Trp Glu Ala Thr Ala Phe

85 90 95

ggc cgt cct atg ctc gcg att gtc ctt gtt gtt tcc ttg gct ttg ttt 336

Gly Arg Pro Met Leu Ala Ile Val Leu Val Val Ser Leu Ala Leu Phe

100 105 110

cct gtg ttc ttg ata cct tct ggt cct tcc atg tgg tta gct ggg atg 384

Pro Val Phe Leu Ile Pro Ser Gly Pro Ser Met Trp Leu Ala Gly Met

115 120 125

att ttt ggt tat ggt ctc ggt ttt gtt att atc atg gtt gga acc acc 432

Ile Phe Gly Tyr Gly Leu Gly Phe Val Ile Ile Met Val Gly Thr Thr

130 135 140

att ggc atg gtt ctc cct tac tta atc ggg ctt atg ttc cgt gat cgc 480

Ile Gly Met Val Leu Pro Tyr Leu Ile Gly Leu Met Phe Arg Asp Arg

145 150 155 160

ctc cat caa tgg tta aaa aga tgg cct cgt caa gct gct gtt cta aga 528

Leu His Gln Trp Leu Lys Arg Trp Pro Arg Gln Ala Ala Val Leu Arg

165 170 175

cta gct gca gaa gga agc tgg ttc cat caa ttc aga gtc gtg gca atc 576

Leu Ala Ala Glu Gly Ser Trp Phe His Gln Phe Arg Val Val Ala Ile

180 185 190

ttt cgg gtt tcc cca ttt cct tac acg att ttt aac tac gca atc gtc 624

Phe Arg Val Ser Pro Phe Pro Tyr Thr Ile Phe Asn Tyr Ala Ile Val

195 200 205

gtg aca agc atg aga ttc tgg cct tac ttc ttc gga tcc ata gca gga 672

Val Thr Ser Met Arg Phe Trp Pro Tyr Phe Phe Gly Ser Ile Ala Gly

210 215 220

atg ata cca gaa gct ttc atc tac att tac agc ggt cgg tta atc aga 720

Met Ile Pro Glu Ala Phe Ile Tyr Ile Tyr Ser Gly Arg Leu Ile Arg

225 230 235 240

aca ttc gca gat gtg caa tac gga cat caa cgt ttg aca aca gtg gag 768

Thr Phe Ala Asp Val Gln Tyr Gly His Gln Arg Leu Thr Thr Val Glu

245 250 255

att gtg tac aat gta atc tcc tta gtc att gcg gtt gtg acc act gtt 816

Ile Val Tyr Asn Val Ile Ser Leu Val Ile Ala Val Val Thr Thr Val

260 265 270

gct ttc act gtg tac gcg aaa aga gct ttg aga gag ctt caa aac gca 864

Ala Phe Thr Val Tyr Ala Lys Arg Ala Leu Arg Glu Leu Gln Asn Ala

275 280 285

gaa gct aat gaa gat gaa gaa gtt caa gta aga aaa gtg aga ttc gag 912

Glu Ala Asn Glu Asp Glu Glu Val Gln Val Arg Lys Val Arg Phe Glu

290 295 300

atg aag aac gta gtt cag cac gaa gaa gat aat cat cag cgt ttg cct 960

Met Lys Asn Val Val Gln His Glu Glu Asp Asn His Gln Arg Leu Pro

305 310 315 320

tag 963

<210>86

<211>320

<212>PRT

＜213〉Arabidopis thaliana

<400>86

Met Ser Asn Pro Leu Lys Glu Ser Arg Glu Asp Ile Ala Asn Ser Thr

1 5 10 15

Pro His Met Arg Asp Asn Glu Tyr Val Arg Leu Val Val Ala His Glu

20 25 30

Ala Ser Pro Ala Glu Thr Val Leu Ser Leu Ser Gln Ser Glu Val Gln

35 40 45

Ser Lys Lys Phe Met Trp Trp Leu Lys Ala Leu Gly Ile Cys Ala Val

50 55 60

Ala Leu Leu Leu Thr Leu Val Phe Gly Lys Trp Gly Val Pro Phe Val

65 70 75 80

Phe Gln Lys Val Leu Ile Pro Ile Leu Gln Trp Glu Ala Thr Ala Phe

85 90 95

Gly Arg Pro Met Leu Ala Ile Val Leu Val Val Ser Leu Ala Leu Phe

100 105 110

Pro Val Phe Leu Ile Pro Ser Gly Pro Ser Met Trp Leu Ala Gly Met

115 120 125

lle Phe Gly Tyr Gly Leu Gly Phe Val Ile Ile Met Val Gly Thr Thr

130 135 140

Ile Gly Met Val Leu Pro Tyr Leu Ile Gly Leu Met Phe Arg Asp Arg

145 150 155 160

Leu His Gln Trp Leu Lys Arg Trp Pro Arg Gln Ala Ala Val Leu Arg

165 170 175

Leu Ala Ala Glu Gly Ser Trp Phe His Gln Phe Arg Val Val Ala Ile

180 185 190

Phe Arg Val Ser Pro Phe Pro Tyr Thr Ile Phe Asn Tyr Ala Ile Val

195 200 205

Val Thr Ser Met Arg Phe Trp Pro Tyr Phe Phe Gly Ser Ile Ala Gly

210 215 220

Met Ile Pro Glu Ala Phe Ile Tyr Ile Tyr Ser Gly Arg Leu Ile Arg

225 230 235 240

Thr Phe Ala Asp Val Gln Tyr Gly His Gln Arg Leu Thr Thr Val Glu

245 250 255

Ile Val Tyr Asn Val Ile Ser Leu Val Ile Ala Val Val Thr Thr Val

260 265 270

Ala Phe Thr Val Tyr Ala Lys Arg Ala Leu Arg Glu Leu Gln Asn Ala

275 280 285

Glu Ala Asn Glu Asp Glu Glu Val Gln Val Arg Lys Val Arg Phe Glu

290 295 300

Met Lys Asn Val Val Gln His Glu Glu Asp Asn His Gln Arg Leu Pro

305 310 315 320

<210>87

<211>1716

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(1716)

<400>87

atg atg aac aag gaa tcg ttt gga gct tgc ttg ctt ctt acg ctt ccc 48

Met Met Asn Lys Glu Ser Phe Gly Ala Cys Leu Leu Leu Thr Leu Pro

1 5 10 15

gaa gat gtg ttt gct gtt atc tct cgt ttt ctt tct cca agc gac att 96

Glu Asp Val Phe Ala Val Ile Ser Arg Phe Leu Ser Pro Ser Asp Ile

20 25 30

tgc aat cta atc ttg tgc ggc aaa agt ctt tgt gcc ctt gtc gat tcc 144

Cys Asn Leu Ile Leu Cys Gly Lys Ser Leu Cys Ala Leu Val Asp Ser

35 40 45

gag aag acg tgg ctt gtg caa tgt gaa gaa gta aaa gtt ctt cct ttg 192

Glu Lys Thr Trp Leu Val Gln Cys Glu Glu Val Lys Val Leu Pro Leu

50 55 60

att gaa cta gtc caa tgg cga atc ggg atc tct tct tac aag gcc ctt 240

Ile Glu Leu Val Gln Trp Arg Ile Gly Ile Ser Ser Tyr Lys Ala Leu

65 70 75 80

tgt agg ttt ctt gtg gag gtg gtg aag ccg ctt ctt ggg att tgg gtg 288

Cys Arg Phe Leu Val Glu Val Val Lys Pro Leu Leu Gly Ile Trp Val

85 90 95

caa gaa aac cct gaa ctt ggg aat gtt gtt tat gtg atg cct ggt ttc 336

Gln Glu Asn Pro Glu Leu Gly Asn Val ValTyr Val Met Pro Gly Phe

100 105 110

ttg tct gtt gtt ggg tgc cgg ata att cca caa aag gtt gct cct ttg 384

Leu Ser Val Val Gly Cys Arg Ile Ile Pro Gln Lys Val Ala Pro Leu

115 120 125

tgg att caa gag ggc caa gtc aag tgg tca ccg gtg ttt gag ata att 432

Trp Ile Gln Glu Gly Gln Val Lys Trp Ser Pro Val Phe Glu Ile Ile

130 135 140

tgc ggc ttt gat ggc tct aag ggt ttt ttc ctc cat gga aga gac aaa 480

Cys Gly Phe Asp Gly Ser Lys Gly Phe Phe Leu His Gly Arg Asp Lys

145 150 155 160

caa ggt agt ttc tta tac cct ggt ttc gtt atg gac atc gag aag agt 528

Gln Gly Ser Phe Leu Tyr Pro Gly Phe Val Met Asp Ile Glu Lys Ser

165 170 175

tgc aat gtg ctt cta ctc gaa gtt gag ccg agg tca gag aag agt tcg 576

Cys Asn Val Leu Leu Leu Glu Val Glu Pro Arg Ser Glu Lys Ser Ser

180 185 190

tgc aat gag att gag aga gaa gta ggg gat cca ttt gga gat cta gac 624

Cys Asn Glu Ile Glu Arg Glu Val Gly Asp Pro Phe Gly Asp Leu Asp

195 200 205

ttc agt gat aga atg aac tta cta gat ata gtg aca aaa cat gta agt 672

Phe Ser Asp Arg Met Asn Leu Leu Asp Ile Val Thr Lys His Val Ser

210 215 220

cta cga gtc gat gaa cca tta aca gga aat tta ttt ccc acc agg tca 720

Leu Arg Val Asp Glu Pro Leu Thr Gly Asn Leu Phe Pro Thr Arg Ser

225 230 235 240

aaa tat gac gaa gcg atg atg ttg gaa cgc aga aac atg ctc ctt aaa 768

Lys Tyr Asp Glu Ala Met Met Leu Glu Arg Arg Asn Met Leu Leu Lys

245 250 255

atg ctc aaa ttt ggt gga aac tgg aag cac ata aac ttg gag gag gat 816

Met Leu Lys Phe Gly Gly Asn Trp Lys His Ile Asn Leu Glu Glu Asp

260 265 270

gag cag ttg tgt tac aat cat ata gag ata gac ata aaa aaa ttg ttg 864

Glu Gln Leu Cys Tyr Asn His Ile Glu Ile Asp Ile Lys Lys Leu Leu

275 280 285

gaa aat ctt ggt gat gac att gac aac atg gag gat ata gag gat cag 912

Glu Asn Leu Gly Asp Asp Ile Asp Asn Met Glu Asp Ile Glu Asp Gln

290 295 300

ata gag gtt aca cca agg aag aag agc ttt cgc cgg ttt tta aga agt 960

Ile Glu Val Thr Pro Arg Lys Lys Ser Phe Arg Arg Phe Leu Arg Ser

305 310 315 320

ggc att aaa cat att ctt ggg aag ttc agt tct tca aag atc aat tcg 1008

Gly Ile Lys His Ile Leu Gly Lys Phe Ser Ser Ser Lys Ile Asn Ser

325 330 335

cct tcg agc agt gag aca aga cgt tcg aat cgc caa agc ttt ctc agc 1056

Pro Ser Ser Ser Glu Thr Arg Arg Ser Asn Arg Gln Ser Phe Leu Ser

340 345 350

tct ggt aat aca ttt tgc ctt agt ctt aaa gct tca tgc act ttg atg 1104

Ser Gly Asn Thr Phe Cys Leu Ser Leu Lys Ala Ser Cys Thr Leu Met

355 360 365

tct tca tat gaa ggg tgg cca atc atg agc gca gac aac ttt tcc ctt 1152

Ser Ser Tyr Glu Gly Trp Pro Ile Met Ser Ala Asp Asn Phe Ser Leu

370 375 380

cat aaa cta cca atg aag aaa cct ctc gat cac gac gtg tat gcg ggt 1200

His Lys Leu Pro Met Lys Lys Pro Leu Asp His Asp Val Tyr Ala Gly

385 390 395 400

ttg tgg gga gga acg ttt ggc tgg ccc cct ggg aaa gat att gaa gat 1248

Leu Trp Gly Gly Thr Phe Gly Trp Pro Pro Gly Lys Asp Ile Glu Asp

405 410 415

gag tcc ctt ctc tta tta atg ctc act tat gga gaa tct gaa gag ggt 1296

Glu Ser Leu Leu Leu Leu Met Leu Thr Tyr Gly Glu Ser Glu Glu Gly

420 425 430

agt gag aga att ctt ttc ggg acg aaa ata ctc agt tat ttt gct gag 1344

Ser Glu Arg Ile Leu Phe Gly Thr Lys Ile Leu Ser Tyr Phe Ala Glu

435 440 445

cat cct aat gga tcc tca atg ttt gtt gta aat att gac acg cct tcc 1392

His Pro Asn Gly Ser Ser Met Phe Val Val Asn Ile Asp Thr Pro Ser

450 455 460

ctt gag ccg ttt cca ttt gat aca gat gga aga gat ttc gag cat tct 1440

Leu Glu Pro Phe Pro Phe Asp Thr Asp Gly Arg Asp Phe Glu His Ser

465 470 475 480

tac acg gga gag ggt atc gct gac ggt tat gga ttc cga tac ccc ggt 1488

Tyr Thr Gly Glu Gly Ile Ala Asp Gly Tyr Gly Phe Arg Tyr Pro Gly

485 490 495

tca aaa cct ggt tcc ctt ttc gta agc tct aat gat ctt ctt gca ttc 1536

Ser Lys Pro Gly Ser Leu Phe Val Ser Ser Asn Asp Leu Leu Ala Phe

500 505 510

gtt tgg caa gga act gaa gat gtg att aca ttg caa aga ata aac ctt 1584

Val Trp Gln Gly Thr Glu Asp Val Ile Thr Leu Gln Arg Ile Asn Leu

515 520 525

gga gag atc ttg aag aag agt tta ggt tct tgt gtt tca cct ttg ctt 1632

Gly Glu Ile Leu Lys Lys Ser Leu Gly Ser Cys Val Ser Pro Leu Leu

530 535 540

cca aca aag aat ttt aca tat act aaa agg tct tac tca aac gtg ttt 1680

Pro Thr Lys Ash Phe Thr Tyr Thr Lys Arg Ser Tyr Ser Asn Val Phe

545 550 555 560

gcc aag tca tcg acc tat tcg tct tcc tcc gag taa 1716

Ala Lys Ser Ser Thr Tyr Ser Ser Ser Ser Glu

565 570

<210>88

<211>571

<212>PRT

＜213〉Arabidopis thaliana

<400>88

Met Met Asn Lys Glu Ser Phe Gly Ala Cys Leu Leu Leu Thr Leu Pro

1 5 10 15

Glu Asp Val Phe Ala Val Ile Ser Arg Phe Leu Ser Pro Ser Asp Ile

20 25 30

Cys Asn Leu Ile Leu Cys Gly Lys Ser Leu Cys Ala Leu Val Asp Ser

35 40 45

Glu Lys Thr Trp Leu Val Gln Cys Glu Glu Val Lys Val Leu Pro Leu

50 55 60

Ile Glu Leu Val Gln Trp Arg Ile Gly Ile Ser Ser Tyr Lys Ala Leu

65 70 75 80

Cys Arg Phe Leu Val Glu Val Val Lys Pro Leu Leu Gly Ile Trp Val

85 90 95

Gln Glu Asn Pro Glu Leu Gly Asn Val Val Tyr Val Met Pro Gly Phe

100 105 110

Leu Ser Val Val Gly Cys Arg Ile Ile Pro Gln Lys Val Ala Pro Leu

115 120 125

Trp Ile Gln Glu Gly Gln Val Lys Trp Ser Pro Val Phe Glu Ile Ile

130 135 140

Cys Gly Phe Asp Gly Ser Lys Gly Phe Phe Leu His Gly Arg Asp Lys

145 150 155 160

Gln Gly Ser Phe Leu Tyr Pro Gly Phe Val Met Asp Ile Glu Lys Ser

165 170 175

Cys Asn Val Leu Leu Leu Glu Val Glu Pro Arg Ser Glu Lys Ser Ser

180 185 190

Cys Asn Glu Ile Glu Arg Glu Val Gly Asp Pro Phe Gly Asp Leu Asp

195 200 205

Phe Ser Asp Arg Met Asn Leu Leu Asp Ile Val Thr Lys His Val Ser

210 215 220

Leu Arg Val Asp Glu Pro Leu Thr Gly Asn Leu Phe Pro Thr Arg Ser

225 230 235 240

Lys Tyr Asp Glu Ala Met Met Leu Glu Arg Arg Asn Met Leu Leu Lys

245 250 255

Met Leu Lys Phe Gly Gly Asn Trp Lys His Ile Asn Leu Glu Glu Asp

260 265 270

Glu Gln Leu Cys Tyr Asn His Ile Glu Ile Asp Ile Lys Lys Leu Leu

275 280 285

Glu Asn Leu Gly Asp Asp Ile Asp Asn Met Glu Asp Ile Glu Asp Gln

290 295 300

Ile Glu Val Thr Pro Arg Lys Lys Ser Phe Arg Arg Phe Leu Arg Ser

305 310 315 320

Gly Ile Lys His Ile Leu Gly Lys Phe Ser Ser Ser Lys Ile Asn Ser

325 330 335

Pro Ser Ser Ser Glu Thr Arg Arg Ser Asn Arg Gln Ser Phe Leu Ser

340 345 350

Ser Gly Asn Thr Phe Cys Leu Ser Leu Lys Ala Ser Cys Thr Leu Met

355 360 365

Ser Ser Tyr Glu Gly Trp Pro Ile Met Ser Ala Asp Asn Phe Ser Leu

370 375 380

His Lys Leu Pro Met Lys Lys Pro Leu Asp His Asp Val Tyr Ala Gly

385 390 395 400

Leu Trp Gly Gly Thr Phe Gly Trp Pro Pro Gly Lys Asp Ile Glu Asp

405 410 415

Glu Ser Leu Leu Leu Leu Met Leu Thr Tyr Gly Glu Ser Glu Glu Gly

420 425 430

Ser Glu Arg Ile Leu Phe Gly Thr Lys Ile Leu Ser Tyr Phe Ala Glu

435 440 445

His Pro Asn Gly Ser Ser Met Phe Val ValAsn Ile Asp Thr Pro Ser

450 455 460

Leu Glu Pro Phe Pro Phe Asp Thr Asp Gly Arg Asp Phe Glu His Ser

465 470 475 480

Tyr Thr Gly Glu Gly Ile Ala Asp Gly Tyr Gly Phe Arg Tyr Pro Gly

485 490 495

Ser Lys Pro Gly Ser Leu Phe Val Ser Ser Asn Asp Leu Leu Ala Phe

500 505 510

Val Trp Gln Gly Thr Glu Asp Val Ile Thr Leu Gln Arg Ile Asn Leu

515 520 525

Gly Glu Ile Leu Lys Lys Ser Leu Gly Ser Cys Val Ser Pro Leu Leu

530 535 540

Pro Thr Lys Asn Phe Thr Tyr Thr Lys Arg Ser Tyr Ser Asn Val Phe

545 550 555 560

Ala Lys Ser Ser Thr Tyr Ser Ser Ser Ser Glu

565 570

<210>89

<211>1632

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(1632)

<400>89

atg gat gta gga cga tgc ccc act tgt caa tct cct cta aaa tac aca 48

Met Asp Val Gly Arg Cys Pro Thr Cys Gln Ser Pro Leu Lys Tyr Thr

1 5 10 15

aaa ctc tca tcg aaa tgt gat gcg tgc tac gaa gat tac cat gac aac 96

Lys Leu Ser Ser Lys Cys Asp Ala Cys Tyr Glu Asp Tyr His Asp Asn

20 25 30

aaa caa ggc tac aag tgt gat tac tgc tcg ttt tgt ctc cac gag gaa 144

Lys Gln Gly Tyr Lys Cys Asp Tyr Cys Ser Phe Cys Leu His Glu Glu

35 40 45

tgc att aac gct aac ctt cct tct cgt cat aaa cac cct ctc aag gtc 192

Cys Ile Asn Ala Asn Leu Pro Ser Arg His Lys His Pro Leu Lys Val

50 55 60

aca aat agc tat aac tca aag tta tgt tat ctt tgt gaa acc aaa agg 240

Thr Asn Ser Tyr Asn Ser Lys Leu Cys Tyr Leu Cys Glu Thr Lys Arg

65 70 75 80

tat cct caa ata ctt tat cac tgt tca caa tgt tct ttc agt ata tgt 288

Tyr Pro Gln Ile Leu Tyr His Cys Ser Gln Cys Ser Phe Ser Ile Cys

85 90 95

atc cct tgt gcg aga aaa cct atc atc ttt gac caa act aag gct cat 336

Ile Pro Cys Ala Arg Lys Pro Ile Ile Phe Asp Gln Thr Lys Ala His

100 105 110

gac cat gaa ctc gtc caa gta atg ata aaa atc ccc ttc act tgt gat 384

Asp His Glu Leu Val Gln Val Met Ile Lys Ile Pro Phe Thr Cys Asp

115 120 125

gca tgt ggg ttg tgt agt aca gaa tat ccg tgt ctt tgt ctt caa tgt 432

Ala Cys Gly Leu Cys Ser Thr Glu Tyr Pro Cys Leu Cys Leu Gln Cys

130 135 140

tgt ttc atg atc cat cga gct tgc atc ttc tta cca cgt gtt ata tac 480

Cys Phe Met Ile His Arg Ala Cys Ile Phe Leu Pro Arg Val Ile Tyr

145 150 155 160

atc aac cgt cat gac cat cgt atc tct cat gtt tct tct ctt tcc act 528

Ile Asn Arg His Asp His Arg Ile Ser His Val Ser Ser Leu Ser Thr

165 170 175

ggg aag tgg att tgt agg gta tgt tat gag cat gtc aat gaa gac tat 576

Gly Lys Trp Ile Cys Arg Val Cys Tyr Glu His Val Asn Glu Asp Tyr

180 185 190

ggg gca tat gct tgc tcg att tgt tct tat ttt ttt cat tca aaa tgt 624

Gly Ala Tyr Ala Cys Ser Ile Cys Ser Tyr Phe Phe His Ser Lys Cys

195 200 205

gca aca aat aca aac att tgg gat ggg aga gaa ctt gaa ggg gta ccc 672

Ala Thr Asn Thr Asn Ile Trp Asp Gly Arg Glu Leu Glu Gly Val Pro

210 215 220

gaa gaa gat gaa aaa gaa gaa atc aaa cca ttc gaa gtg ata gat caa 720

Glu Glu Asp Glu Lys Glu Glu Ile Lys Pro Phe Glu Val Ile Asp Gln

225 230 235 240

acc cta ata aaa cat ttt agc cat gaa cac aat ctc aag gct tct ata 768

Thr Leu Ile Lys His Phe Ser His Glu His Asn Leu Lys Ala Ser Ile

245 250 255

tca tca tcg aga cta gag gaa cca aga gaa cgt tgt tgt gca tgc act 816

Ser Ser Ser Arg Leu Glu Glu Pro Arg Glu Arg Cys Cys Ala Cys Thr

260 265 270

ctt cct ttt tat tct gaa atg tgc tat aaa tgc aca caa tgt gat ttt 864

Leu Pro Phe Tyr Ser Glu Met Cys Tyr Lys Cys Thr Gln Cys Asp Phe

275 280 285

att ctt cat gat tca tgc gct aat ctt cct cta aag aaa aga cat gag 912

Ile Leu His Asp Ser Cys Ala Asn Leu Pro Leu Lys Lys Arg His Glu

290 295 300

ata agc aca cac aaa ctt atg tta tgt acc aaa agt cag ctt ggc gca 960

Ile Ser Thr His Lys Leu Met Leu Cys Thr Lys Ser Gln Leu Gly Ala

305 310 315 320

aac ttc gaa aat tcg ttt ttt tgt gtt aca tgt caa cgt tat tgc act 1008

Asn Phe Glu Asn Ser Phe Phe Cys Val Thr Cys Gln Arg Tyr Cys Thr

325 330 335

ggt ttc tca tat aat att act ggg gat gaa aac aac acc caa cat agc 1056

Gly Phe Ser Tyr Asn Ile Thr Gly Asp Glu Asn Asn Thr Gln His Ser

340 345 350

att gat gtg cga tgt gct tca att tcc gat gca ttt aaa cat gaa agt 1104

Ile Asp Val Arg Cys Ala Ser Ile Ser Asp Ala Phe Lys His Glu Ser

355 360 365

cat cca cat tgg cta ttt gta ttt ttt gat ttg ttt tac tca agg tgt 1152

His Pro His Trp Leu Phe Val Phe Phe Asp Leu Phe Tyr Ser Arg Cys

370 375 380

gaa ggt tgt gac ttg aat tgt tgg ttt tat cta cgt tgc aag gat aaa 1200

Glu Gly Cys Asp Leu Asn Cys Trp Phe Tyr Leu Arg Cys Lys Asp Lys

385 390 395 400

gat gat tgt ggt ggc tac aat tta tgt ttc aca tgc gca act tta ccc 1248

Asp Asp Cys Gly Gly Tyr Asn Leu Cys Phe Thr Cys Ala Thr Leu Pro

405 410 415

aca ttg gta aga gat aaa tat gat gat cat cct ctt tca tta tgc tac 1296

Thr Leu Val Arg Asp Lys Tyr Asp Asp His Pro Leu Ser Leu Cys Tyr

420 425 430

ggt gag aaa aat gtg aat gcg aga tat tgt tgc ggg ata tgc gaa gag 1344

Gly Glu Lys Asn Val Asn Ala Arg Tyr Cys Cys Gly Ile Cys Glu Glu

435 440 445

gat gta cat tca aag agt tgg ttc tac aaa tgc aat gaa tgt ggc tcc 1392

Asp Val His Ser Lys Ser Trp Phe Tyr Lys Cys Asn Glu Cys Gly Ser

450 455 460

act ctt cat act aaa tgt gta ttt caa aat cta ata cac tca agg tct 1440

Thr Leu His Thr Lys Cys Val Phe Gln Asn Leu Ile His Ser Arg Ser

465 470 475 480

gga tac agt tta gtg att ggt aac aag ggg tca ttt gat ttg ctt cca 1488

Gly Tyr Ser Leu Val Ile Gly Asn Lys Gly Ser Phe Asp Leu Leu Pro

485 490 495

aac aat cgt ctc tct cgg cca att tgc tat tta tgc aag atc cgt tgt 1536

Asn Asn Arg Leu Ser Arg Pro Ile Cys Tyr Leu Cys Lys Ile Arg Cys

500 505 510

atg ggt gat ttt gtt ctc cac aaa aag gcg gat aca agt ata ttc ata 1584

Met Gly Asp Phe Val Leu His Lys Lys Ala Asp Thr Ser Ile Phe Ile

515 520 525

tgc tgc tct tgt gga ttt aat tat aaa gat ttg gtt agg agg tat tga 1632

Cys Cys Ser Cys Gly Phe Asn Tyr Lys Asp Leu Val Arg Arg Tyr

530 535 540

<210>90

<211>543

<212>PRT

＜213〉Arabidopis thaliana

<400>90

Met Asp Val Gly Arg Cys Pro Thr Cys Gln Ser Pro Leu Lys Tyr Thr

1 5 10 15

Lys Leu Ser Ser Lys Cys Asp Ala Cys Tyr Glu Asp Tyr His Asp Asn

20 25 30

Lys Gln Gly Tyr Lys Cys Asp Tyr Cys Ser Phc Cys Leu His Glu Glu

35 40 45

Cys Ile Asn Ala Asn Leu Pro Ser Arg His Lys His Pro Leu Lys Val

50 55 60

Thr Asn Ser Tyr Asn Ser Lys Leu Cys Tyr Leu Cys Glu Thr Lys Arg

65 70 75 80

Tyr Pro Gln Ile Leu Tyr His Cys Ser Gln Cys Ser Phe Ser Ile Cys

85 90 95

Ile Pro Cys Ala Arg Lys Pro Ile Ile Phe Asp Gln Thr Lys Ala His

100 105 110

Asp His Glu Leu Val Gln Val Met Ile Lys Ile Pro Phe Thr Cys Asp

115 120 125

Ala Cys Gly Leu Cys Ser Thr Glu Tyr Pro Cys Leu Cys Leu Gln Cys

130 135 140

Cys Phe Met Ile His Arg Ala Cys Ile Phe Leu Pro Arg Val Ile Tyr

145 150 155 160

Ile Asn Arg His Asp His Arg Ile Ser His Val Ser Ser Leu Ser Thr

165 170 175

Gly Lys Trp Ile Cys Arg Val Cys Tyr Glu His Val Asn Glu Asp Tyr

180 185 190

Gly Ala Tyr Ala Cys Ser Ile Cys Ser Tyr Phe Phe His Ser Lys Cys

195 200 205

Ala Thr Asn Thr Asn Ile Trp Asp Gly Arg Glu Leu Glu Gly Val Pro

210 215 220

Glu Glu Asp Glu Lys Glu Glu Ile Lys Pro Phe Glu Val Ile Asp Gln

225 230 235 240

Thr Leu Ile Lys His Phe Ser His Glu His Asn Leu Lys Ala Ser Ile

245 250 255

Ser Ser Ser Arg Leu Glu Glu Pro Arg Glu Arg Cys Cys Ala Cys Thr

260 265 270

Leu Pro Phe Tyr Ser Glu Met Cys Tyr Lys Cys Thr Gln Cys Asp Phe

275 280 285

Ile Leu His Asp Ser Cys Ala Asn Leu Pro Leu Lys Lys Arg His Glu

290 295 300

Ile Ser Thr His Lys Leu Met Leu Cys Thr Lys Ser Gln Leu Gly Ala

305 310 315 320

Asn Phe Glu Asn Ser Phe Phe Cys Val Thr Cys Gln Arg Tyr Cys Thr

325 330 335

Gly Phe Ser Tyr Asn Ile Thr Gly Asp Glu Asn Asn Thr Gln His Ser

340 345 350

Ile Asp Val Arg Cys Ala Ser Ile Ser Asp Ala Phe Lys His Glu Ser

355 360 365

His Pro His Trp Leu Phe Val Phe Phe Asp Leu Phe Tyr Ser Arg Cys

370 375 380

Glu Gly Cys Asp Leu Asn Cys Trp Phe Tyr Leu Arg Cys Lys Asp Lys

385 390 395 400

Asp Asp Cys Gly Gly Tyr Asn Leu Cys Phe Thr Cys Ala Thr Leu Pro

405 410 415

Thr Leu Val Arg Asp Lys Tyr Asp Asp His Pro Leu Ser Leu Cys Tyr

420 425 430

Gly Glu Lys Asn Val Asn Ala Arg Tyr Cys Cys Gly Ile Cys Glu Glu

435 440 445

Asp Val His Ser Lys Ser Trp Phe Tyr Lys Cys Asn Glu Cys Gly Ser

450 455 460

Thr Leu His Thr Lys Cys Val Phe Gln Asn Leu Ile His Ser Arg Ser

465 470 475 480

Gly Tyr Ser Leu Val Ile Gly Asn Lys Gly Ser Phe Asp Leu Leu Pro

485 490 495

Asn Asn Arg Leu Ser Arg Pro Ile Cys Tyr Leu Cys Lys Ile Arg Cys

500 505 510

Met Gly Asp Phe Val Leu His Lys Lys Ala Asp Thr Ser Ile Phe Ile

515 520 525

Cys Cys Ser Cys Gly Phe Asn Tyr Lys Asp Leu Val Arg Arg Tyr

530 535 540

<210>91

<211>2460

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(2460)

<400>91

atg gaa gca ttt ctt aag gaa ttc gga gat tat tat gga tac cca gat 48

Met Glu Ala Phe Leu Lys Glu Phe Gly Asp Tyr Tyr Gly Tyr Pro Asp

1 5 10 15

ggt ccc aag aac att caa gag atc cgc gac acc gaa ttc aag aga tta 96

Gly Pro Lys Asn Ile Gln Glu Ile Arg Asp Thr Glu Phe Lys Arg Leu

20 25 30

gat aaa ggt gtt gta tac ttg gat cat gct ggt tct act ttg tat tct 144

Asp Lys Gly Val Val Tyr Leu Asp His Ala Gly Ser Thr Leu Tyr Ser

35 40 45

gag ttg cag atg gaa tat att ttt aag gac ttc aca agc aat gtt ttt 192

Glu Leu Gln Met Glu Tyr Ile Phe Lys Asp Phe Thr Ser Asn Val Phe

50 55 60

gga aat cca cat agt caa agt gat atc agt tcg gcc acc agt gac ctt 240

Gly Asn Pro His Ser Gln Ser Asp Ile Ser Ser Ala Thr Ser Asp Leu

65 70 75 80

ata gcg gat gct cga cat cag gtg ctt gaa tac ttt aat gca tct cct 288

Ile Ala Asp Ala Arg His Gln Val Leu Glu Tyr Phe Asn Ala Ser Pro

85 90 95

gaa gat tac agt tgc tta ttc acc tcc gga gcc aca gca gcg ctg aag 336

Glu Asp Tyr Ser Cys Leu Phe Thr Ser Gly Ala Thr Ala Ala Leu Lys

100 105 1l0

ctt gtc gga gag act ttt ccg tgg acc caa gac agt aat ttt ttg tat 384

Leu Val Gly Glu Thr Phe Pro Trp Thr Gln Asp Ser Asn Phe Leu Tyr

115 120 125

acc atg gag aat cac aac agt gta ctt ggt att agg gaa tat gca tta 432

Thr Met Glu Asn His Asn Ser Val Leu Gly Ile Arg Glu Tyr Ala Leu

130 135 140

gct caa ggt gct tca gca tgt gca gtg gat att gaa gag gca gct aac 480

Ala Gln Gly Ala Ser Ala Cys Ala Val Asp Ile Glu Glu Ala Ala Asn

145 150 155 160

caa cca ggc cag ctt aca aat tca gga cca tct atc aag gta aag cat 528

Gln Pro Gly Gln Leu Thr Asn Ser Gly Pro Ser Ile Lys Val Lys His

165 170 175

cgt gct gtg cag atg aga aac act tct aaa ctc caa aag gaa gag tca 576

Arg Ala Val Gln Met Arg Asn Thr Ser Lys Leu Gln Lys Glu Glu Ser

180 185 190

aga gga aat gcc tat aat cta ttt gct ttc ccc tcg gag tgc aat ttt 624

Arg Gly Asn Ala Tyr Asn Leu Phe Ala Phe Pro Ser Glu Cys Asn Phe

195 200 205

tct ggc ctg agg ttt aat cta gat ctg gtg aag ttg atg aaa gaa aat 672

Ser Gly Leu Arg Phe Asn Leu Asp Leu Val Lys Leu Met Lys Glu Asn

210 215 220

act gag acc gtg cta caa ggc tcc ccc ttt agc aag agc aag cgg tgg 720

Thr Glu Thr Val Leu Gln Gly Ser Pro Phe Ser Lys Ser Lys Arg Trp

225 230 235 240

atg gtc ttg att gat gct gca aag ggt tgt gct aca cta cca cct gat 768

Met Val Leu Ile Asp Ala Ala Lys Gly Cys Ala Thr Leu Pro Pro Asp

245 250 255

tta tcg gag tat cct gca gat ttt gtt gtt ctg tca ttc tac aag tta 816

Leu Ser Glu Tyr Pro Ala Asp Phe Val Val Leu Ser Phe Tyr Lys Leu

260 265 270

ttt ggt tat cct act ggg ctt ggc gct ctc ctt gta cgg aat gat gca 864

Phe Gly Tyr Pro Thr Gly Leu Gly Ala Leu Leu Val Arg Asn Asp Ala

275 280 285

gcc aaa ttg ctc aaa aag act tat ttt agt gga ggc act gtt gct gct 912

Ala Lys Leu Leu Lys Lys Thr Tyr Phe Ser Gly Gly Thr Val Ala Ala

290 295 300

tca att gct gac atc gac ttt gta aaa aga agg gaa agg gtg gag gag 960

Ser Ile Ala Asp Ile Asp Phe Val Lys Arg Arg Glu Arg Val Glu Glu

305 310 315 320

ttt ttt gag gat ggt tct gct tca ttc ctg agc ata gca gcc atc cgt 1008

Phe Phe Glu Asp Gly Ser Ala Ser Phe Leu Ser Ile Ala Ala Ile Arg

325 330 335

cat ggc ttc aaa tta ctc aag tcg ctt aca cct tct gca att tgg atg 1056

His Gly Phe Lys Leu Leu Lys Ser Leu Thr Pro Ser Ala Ile Trp Met

340 345 350

cac aca acg tca ctt tcc ata tat gtg aaa aag aag ctt cag gct tta 1104

His Thr Thr Ser Leu Ser Ile Tyr ValLys Lys Lys Leu Gln Ala Leu

355 360 365

cga cat gga aac ggg gct gct gta tgt gtt ctg tat ggc agt gaa aat 1152

Arg His Gly Asn Gly Ala Ala Val Cys Val Leu Tyr Gly Ser Glu Asn

370 375 380

ctg gag tta tct tca cat aaa tca ggc cca acg gtt aca ttc aac ttg 1200

Leu Glu Leu Ser Ser His Lys Ser Gly Pro Thr Val Thr Phe Asn Leu

385 390 395 400

aaa aga cct gat ggc tct tgg ttt ggc tac ttg gag gtg gag aag ctt 1248

Lys Arg Pro Asp Gly Ser Trp Phe Gly Tyr Leu Glu Val Glu Lys Leu

405 410 415

gct tct tta tct gga att cag tta cgg aca gga tgt ttt tgc aat cct 1296

Ala Ser Leu Ser Gly Ile Gln Leu Arg Thr Gly Cys Phe Cys Asn Pro

420 425 430

ggc gca tgt gca aag tat ctc gag tta tcc cat tct gag cta cgg tct 1344

Gly Ala Cys Ala Lys Tyr Leu Glu Leu Ser His Ser Glu Leu Arg Ser

435 440 445

aat gta gag gct ggg cat att tgc tgg gat gac aat gat gtg ata aat 1392

Asn Val Glu Ala Gly His Ile Cys Trp Asp Asp Asn Asp Val Ile Asn

450 455 460

gga aaa cca aca ggg gct gtt agg gtt tcg ttt ggt tat atg tca acc 1440

Gly Lys Pro Thr Gly Ala Val Arg Val Ser Phe Gly Tyr Met Ser Thr

465 470 475 480

ttt gaa gat gcc aag aaa ttt att gat ttc atc ata agt tca ttt gct 1488

Phe Glu Asp Ala Lys Lys Phe Ile Asp Phe Ile Ile Ser Ser Phe Ala

485 490 495

tca cct cca aag aag act ggg aat gga acc gtc gtc agt gga agg ttt 1536

Ser Pro Pro Lys Lys Thr Gly Asn Gly Thr Val Val Ser Gly Arg Phe

500 505 510

cct caa ctt cct agt gaa gac ctt gaa agt aaa gaa tct ttt cca agc 1584

Pro Gln Leu Pro Ser Glu Asp Leu Glu Ser Lys Glu Ser Phe Pro Ser

515 520 525

cac tac ctt aag tca att act gta tac ccg atc aag tca tgt gct gga 1632

His Tyr Leu Lys Ser Ile Thr Val Tyr Pro Ile Lys Ser Cys Ala Gly

530 535 540

ttt tct gtg ata cgt tgg cca ctt tgc aga aca ggc ctg ctg cat gat 1680

Phe Ser Val Ile Arg Trp Pro Leu Cys Arg Thr Gly Leu Leu His Asp

545 550 555 560

cga gaa tgg atg gtt cag ggt ctg acc ggt gaa att ctt acc caa aag 1728

Arg Glu Trp Met Val Gln Gly Leu Thr Gly Glu Ile Leu Thr Gln Lys

565 570 575

aag gtg cct gag atg tct ctt ata aaa acc ttt atc gac ctt gag gaa 1776

Lys Val Pro Glu Met Ser Leu Ile Lys Thr Phe lle Asp Leu Glu Glu

580 585 590

gga cta ctg tct gta gaa tct tct cgc tgc gaa gac aag ttg cac atc 1824

Gly Leu Leu Ser Val Glu Ser Ser Arg Cys Glu Asp Lys Leu His Ile

595 600 605

aga atc aag tct gat tca tat aac ccg agg aac gat gag ttt gat tca 1872

Arg Ile Lys Ser Asp Ser Tyr Asn Pro Arg Asn Asp Glu Phe Asp Ser

610 615 620

cat gcc aac ata ctt gaa aac cgt aat gag gaa act aga atc aat cgt 1920

His Ala Asn Ile Leu Glu Asn Arg Asn Glu Glu Thr Arg Ile Asn Arg

625 630 635 640

tgg ttc acc aat gcc att ggt cga caa tgc aag ttg cta cgg tat tct 1968

Trp Phe Thr Asn Ala Ile Gly Arg Gln Cys Lys Leu Leu Arg Tyr Ser

645 650 655

agc tct act tcc aaa gac tgc ttg aac aga aac aag agt cct ggt ttg 2016

Ser Ser Thr Ser Lys Asp Cys Leu Asn Arg Asn Lys Ser Pro Gly Leu

660 665 670

tgc aga gat ttg gaa agc aat atc aac ttt gct aat gaa gct cag ttc 2064

Cys Arg Asp Leu Glu Ser Asn Ile Asn Phe Ala Asn Glu Ala Gln Phe

675 680 685

ttg tta atc tcc gag gag agt gtt gct gac cta aac aga aga tta gaa 2112

Leu Leu Ile Ser Glu Glu Ser Val Ala Asp Leu Asn Arg Arg Leu Glu

690 695 700

gca aaa gac gag gat tac aaa cgg gct cat gaa aaa ctc aat cca cat 2160

Ala Lys Asp Glu Asp Tyr Lys Arg Ala His Glu Lys Leu Asn Pro His

705 710 715 720

agg ttc aga cca aat ctg gtt ata tct gga ggt gaa cca tac ggg gaa 2208

Arg Phe Arg Pro Asn Leu Val Ile Ser Gly Gly Glu Pro Tyr Gly Glu

725 730 735

gat aaa tgg aaa act gtc aag ata gga gac aat cat ttc aca tca ttg 2256

Asp Lys Trp Lys Thr Val Lys Ile Gly Asp Asn His Phe Thr Ser Leu

740 745 750

ggc ggt tgt aac cgg tgc cag atg ata aac ata agt aat gaa gct gga 2304

Gly Gly Cys Asn Arg Cys Gln Met Ile Asn Ile Ser Asn Glu Ala Gly

755 760 765

cta gtg aag aaa tcc aat gag ccc tta aca act tta gct tca tat agg 2352

Leu Val Lys Lys Ser Asn Glu Pro Leu Thr Thr Leu Ala Ser Tyr Arg

770 775 780

aga gta aag gga aag atc ttg ttt gga acg ctt ttg aga tac gag att 2400

Arg Val Lys Gly Lys Ile Leu Phe Gly Thr Leu Leu Arg Tyr Glu Ile

785 790 795 800

gat gag aaa aga caa tgt tgg att gga gtt ggg gaa gaa gtt aat cca 2448

Asp Glu Lys Arg Gln Cys Trp Ile Gly Val Gly Glu Glu Val Asn Pro

805 8l0 815

gat att gaa taa 2460

Asp Ile Glu

<210>92

<211>819

<212>PRT

＜213〉Arabidopis thaliana

<400>92

Met Glu Ala Phe Leu Lys Glu Phe Gly Asp Tyr Tyr Gly Tyr Pro Asp

1 5 10 15

Gly Pro Lys Asn Ile Gln Glu Ile Arg Asp Thr Glu Phe Lys Arg Leu

20 25 30

Asp Lys Gly Val Val Tyr Leu Asp His Ala Gly Ser Thr Leu Tyr Ser

35 40 45

Glu Leu Gln Met Glu Tyr Ile Phe Lys Asp Phe Thr Ser Asn Val Phe

50 55 60

Gly Asn Pro His Ser Gln Ser Asp Ile Ser Ser Ala Thr Ser Asp Leu

65 70 75 80

Ile Ala Asp Ala Arg His Gln Val Leu Glu Tyr Phe Asn Ala Ser Prc

85 90 95

Glu Asp Tyr Ser Cys Leu Phe Thr Ser Gly Ala Thr Ala Ala Leu Lys

100 105 110

Leu Val Gly Glu Thr Phe Pro Trp Thr Gln Asp Ser Asn Phe Leu Tyr

115 120 125

Thr Met Glu Asn His Asn Ser Val Leu Gly Ile Arg Glu Tyr Ala Leu

130 135 140

Ala Gln Gly Ala Ser Ala Cys Ala Val Asp Ile Glu Glu Ala Ala Asn

145 150 155 160

Gln Pro Gly Gln Leu Thr Asn Ser Gly Pro Ser Ile Lys Val Lys His

165 170 175

Arg Ala Val Gln Met Arg Asn Thr Ser Lys Leu Gln Lys Glu Glu Ser

180 185 190

Arg Gly Asn Ala Tyr Asn Leu Phe Ala Phe Pro Ser Glu Cys Asn Phe

195 200 205

Ser Gly Leu Arg Phe Asn Leu Asp Leu Val Lys Leu Met Lys Glu Asn

210 215 220

Thr Glu Thr Val Leu Gln Gly Ser Pro Phe Ser Lys Ser Lys Arg Trp

225 230 235 240

Met Val Leu Ile Asp Ala Ala Lys Gly Cys Ala Thr Leu Pro Pro Asp

245 250 255

Leu Ser Glu Tyr Pro Ala Asp Phe Val Val Leu Ser Phe Tyr Lys Leu

260 265 270

Phe Gly Tyr Pro Thr Gly Leu Gly Ala Leu Leu Val Arg Asn Asp Ala

275 280 285

Ala Lys Leu Leu Lys Lys Thr Tyr Phe Ser Gly Gly Thr Val Ala Ala

290 295 300

Ser Ile Ala Asp Ile Asp Phe Val Lys Arg Arg Glu Arg Val Glu Glu

305 310 315 320

Phe Phe Glu Asp Gly Ser Ala Ser Phe Leu Ser Ile Ala Ala Ile Arg

325 330 335

His Gly Phe Lys Leu Leu Lys Ser Leu Thr Pro Ser Ala Ile Trp Met

340 345 350

His Thr Thr Ser Leu Ser Ile Tyr Val Lys Lys Lys Leu Gln Ala Leu

355 360 365

Arg His Gly Asn Gly Ala Ala Val Cys Val Leu Tyr Gly Ser Glu Asn

370 375 380

Leu Glu Leu Ser Ser His Lys Ser Gly Pro Thr Val Thr Phe Asn Leu

385 390 395 400

Lys Arg Pro Asp Gly Ser Trp Phe Gly Tyr Leu Glu Val Glu Lys Leu

405 410 415

Ala Ser Leu Ser Gly Ile Gln Leu Arg Thr Gly Cys Phe Cys Asn Pro

420 425 430

Gly Ala Cys Ala Lys Tyr Leu Glu Leu Ser His Ser Glu Leu Arg Ser

435 440 445

Asn Val Glu Ala Gly His Ile Cys Trp Asp Asp Asn Asp Val Ile Asn

450 455 460

Gly Lys Pro Thr Gly Ala Val Arg Val Ser Phe Gly Tyr Met Ser Thr

465 470 475 480

Phe Glu Asp Ala Lys Lys Phe Ile Asp Phe Ile Ile Ser Ser Phe Ala

485 490 495

Ser Pro Pro Lys Lys Thr Gly Asn Gly Thr Val Val Ser Gly Arg Phe

500 505 510

Pro Gln Leu Pro Ser Glu Asp Leu Glu Ser Lys Glu Ser Phe Pro Ser

515 520 525

His Tyr Leu Lys Ser Ile Thr Val Tyr Pro Ile Lys Ser Cys Ala Gly

530 535 540

Phe Ser Val Ile Arg Trp Pro Leu Cys Arg Thr Gly Leu Leu His Asp

545 550 555 560

Arg Glu Trp Met Val Gln Gly Leu Thr Gly Glu Ile Leu Thr Gln Lys

565 570 575

Lys Val Pro Glu Met Ser Leu lle Lys Thr Phe Ile Asp Leu Glu Glu

580 585 590

Gly Leu Leu Ser Val Glu Ser Ser Arg Cys Glu Asp Lys Leu His Ile

595 600 605

Arg Ile Lys Ser Asp Ser Tyr Asn Pro Arg Asn Asp Glu Phe Asp Ser

610 615 620

His Ala Asn Ile Leu Glu Asn Arg Asn Glu Glu Thr Arg Ile Asn Arg

625 630 635 640

Trp Phe Thr Asn Ala Ile Gly Arg Gln Cys Lys Leu Leu Arg Tyr Ser

645 650 655

Ser Ser Thr Ser Lys Asp Cys Leu Asn Arg Asn Lys Ser Pro Gly Leu

660 665 670

Cys Arg Asp Leu Glu Ser Asn Ile Asn Phe Ala Asn Glu Ala Gln Phe

675 680 685

Leu Leu Ile Ser Glu Glu Ser ValAla Asp Leu Asn Arg Arg Leu Glu

690 695 700

Ala Lys Asp Glu Asp Tyr Lys Arg Ala His Glu Lys Leu Asn Pro His

705 710 715 720

Arg Phe Arg Pro Asn Leu Val Ile Ser Gly Gly Glu Pro Tyr Gly Glu

725 730 735

Asp Lys Trp Lys Thr Val Lys Ile Gly Asp Asn His Phe Thr Ser Leu

740 745 750

Gly Gly Cys Asn Arg Cys Gln Met Ile Asn Ile Ser Asn Glu Ala Gly

755 760 765

Leu Val Lys Lys Ser Asn Glu Pro Leu Thr Thr Leu Ala Ser Tyr Arg

770 775 780

Arg Val Lys Gly Lys Ile Leu Phe Gly Thr Leu Leu Arg Tyr Glu Ile

785 790 795 800

Asp Glu Lys Arg Gln Cys Trp Ile Gly Val Gly Glu Glu Val Asn Pro

805 810 815

Asp Ile Glu

<210>93

<211>1110

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(1110)

<400>93

atg aag ctt tat tct gtt tcc atc atc atc ttc gtc tta att gct ctc 48

Met Lys Leu Tyr Ser Val Ser Ile Ile Ile Phe Val Leu Ile Ala Leu

1 5 10 15

tcc acc ata gtt aat gct caa caa gct gct aca gat tcc tgc aac tca 96

Ser Thr Ile Val Asn Ala Gln Gln Ala Ala Thr Asp Ser Cys Asn Ser

20 25 30

act cta cct ctc aac gac ctc acc ttc aac acc agc ctc ctt caa tgc 144

Thr Leu Pro Leu Asn Asp Leu Thr Phe Asn Thr Ser Leu Leu Gln Cys

35 40 45

acc gaa gct tgg act ccc caa aat ttc atc ctc cga tat gca aga acg 192

Thr Glu Ala Trp Thr Pro Gln Asn Phe Ile Leu Arg Tyr Ala Arg Thr

50 55 60

gca gag aac aca tgg agc ttt atc tta tcg gcg ccg gat tca agc gct 240

Ala Glu Asn Thr Trp Ser Phe Ile Leu Ser Ala Pro Asp Ser Ser Ala

65 70 75 80

ttc atc ggg atc gga ttc tct acc aac ggt cag atg atc gga agc agc 288

Phe Ile Gly Ile Gly Phe Ser Thr Asn Gly Gln Met Ile Gly Ser Ser

85 90 95

gcg atc gtt ggt tgg ata cct tcc gac ggc ggt tcc ggg act gtg aaa 336

Ala Ile Val Gly Trp Ile Pro Ser Asp Gly Gly Ser Gly Thr Val Lys

100 105 110

ccg tac ttg ctc ggt ggg aaa tct ccc gga gag gtt aat cct gac caa 384

Pro Tyr Leu Leu Gly Gly Lys Ser Pro Gly Glu Val Asn Pro Asp Gln

115 120 125

gga gat cta acg atc gtc aac ggc tcg ttg aag atc gaa tca gtg tcg 432

Gly Asp Leu Thr Ile Val Asn Gly Ser Leu Lys Ile Glu Ser Val Ser

130 135 140

tcg cgt ctt tac atg aga ttt caa ttg acg gcg acg ctg ccg cgg cag 480

Ser Arg Leu Tyr Met Arg Phe Gln Leu Thr Ala Thr Leu Pro Arg Gln

145 150 155 160

agt ctt ctt tac gct gtg gga cct gcc gga ttc ttc cca tct tcg ccg 528

Ser Leu Leu Tyr Ala Val Gly Pro Ala Gly Phe Phe Pro Ser Ser Pro

165 170 175

gat ttt agg ttg aga gag cac cgc ttc gtg acc acc acg acc atc aat 576

Asp Phe Arg Leu Arg Glu His Arg Phe Val Thr Thr Thr Thr Ile Asn

180 185 190

tat aat aca ggt tcg caa agt gtg gtt aaa gtt tca cca cac tct aag 624

Tyr Asn Thr Gly Ser Gln Ser Val Val Lys Val Ser Pro His Ser Lys

195 200 205

cta aag aag aca cat ggg cta atg aac atg ttc ggc tgg gga ata ttg 672

Leu Lys Lys Thr His Gly Leu Met Asn Met Phe Gly Trp Gly Ile Leu

210 215 220

att atc gtt ggc gcc ata gtg gct cga cat atg aag caa tgg gac ccc 720

Ile Ile Val Gly Ala Ile Val Ala Arg His Met Lys Gln Trp Asp Pro

225 230 235 240

act tgg ttc tat gcc cat atc gct ctc caa acc act ggt ttt ctc ctc 768

Thr Trp Phe Tyr Ala His Ile Ala Leu Gln Thr Thr Gly Phe Leu Leu

245 250 255

ggt tta act ggt gtc att tgc ggt ttg gtt ctt gaa aac cgg ctc aag 816

Gly Leu Thr Gly Val Ile Cys Gly Leu Val Leu Glu Asn Arg Leu Lys

260 265 270

gcc aat aat gtt tcc aag cac aaa ggc ctc ggg ata acc ata ctt gtc 864

Ala Asn Asn Val Ser Lys His Lys Gly Leu Gly Ile Thr Ile Leu Val

275 280 285

atg ggc gtt ctt cag atg ctg gca ttg cta gct cgg ccg gat aag caa 912

Met Gly Val Leu Gln Met Leu Ala Leu Leu Ala Arg Pro Asp Lys Gln

290 295 300

tcg aaa tac aga aaa tat tgg aat tgg tat cat cat aac ata gga aga 960

Ser Lys Tyr Arg Lys Tyr Trp Asn Trp Tyr His His Asn Ile Gly Arg

305 310 315 320

ctt ctg atc ata ctg gct att tct aac atc ttc tac ggt att cat ttg 1008

Leu Leu Ile Ile Leu Ala Ile Ser Asn Ile Phe Tyr Gly Ile His Leu

325 330 335

gct aaa gct gga act agt tgg aat ggt ggt tac ggt ttt gcg gtc gcg 1056

Ala Lys Ala Gly Thr Ser Trp Asn Gly Gly Tyr Gly Phe Ala Val Ala

340 345 350

gtc ttg gcc ttg acg gct att gga tta gaa gtt aga aag ttc ttg aaa 1104

Val Leu Ala Leu Thr Ala Ile Gly Leu Glu Val Arg Lys Phe Leu Lys

355 360 365

aag taa 1110

Lys

<210>94

<211>369

<212>PRT

＜213〉Arabidopis thaliana

<400>94

Met Lys Leu Tyr Ser Val Ser Ile Ile Ile Phe Val Leu Ile Ala Leu

1 5 10 15

Ser Thr Ile Val Asn Ala Gln Gln Ala Ala Thr Asp Ser Cys Asn Ser

20 25 30

Thr Leu Pro Leu Asn Asp Leu Thr Phe Asn Thr Ser Leu Leu Gln Cys

35 40 45

Thr Glu Ala Trp Thr Pro Gln Asn Phe Ile Leu Arg Tyr Ala Arg Thr

50 55 60

Ala Glu Asn Thr Trp Ser Phe Ile Leu Ser Ala Pro Asp Ser Ser Ala

65 70 75 80

Phe Ile Gly Ile Gly Phe Ser Thr Asn Gly Gln Met Ile Gly Ser Ser

85 90 95

Ala Ile Val Gly Trp Ile Pro Ser Asp Gly Gly Ser Gly Thr Val Lys

100 105 110

Pro Tyr Leu Leu Gly Gly Lys Ser Pro Gly Glu Val Asn Pro Asp Gln

115 120 125

Gly Asp Leu Thr Ile Val Asn Gly Ser Leu Lys Ile Glu Ser Val Ser

130 135 140

Ser Arg Leu Tyr Met Arg Phe Gln Leu Thr Ala Thr Leu Pro Arg Gln

145 150 155 160

Ser Leu Leu Tyr Ala Val Gly Pro Ala Gly Phe Phe Pro Ser Ser Pro

165 170 175

Asp Phe Arg Leu Arg Glu His Arg Phe Val Thr Thr Thr Thr Ile Asn

180 185 190

Tyr Asn Thr Gly Ser Gln Ser Val Val Lys Val Ser Pro His Ser Lys

195 200 205

Leu Lys Lys Thr His Gly Leu Met Asn Met Phe Gly Trp Gly Ile Leu

210 215 220

Ile Ile Val Gly Ala Ile Val Ala Arg His Met Lys Gln Trp Asp Pro

225 230 235 240

Thr Trp Phe Tyr Ala His Ile Ala Leu Gln Thr Thr Gly Phe Leu Leu

245 250 255

Gly Leu Thr Gly Val Ile Cys Gly Leu Val Leu Glu Asn Arg Leu Lys

260 265 270

Ala Asn Asn Val Ser Lys His Lys Gly Leu Gly Ile Thr Ile Leu Val

275 280 285

Met Gly Val Leu Gln Met Leu Ala Leu Leu Ala Arg Pro Asp Lys Gln

290 295 300

Ser Lys Tyr Arg Lys Tyr Trp Asn Trp Tyr His His Asn Ile Gly Arg

305 310 315 320

Leu Leu Ile Ile Leu Ala Ile Ser Asn Ile Phe Tyr Gly Ile His Leu

325 330 335

Ala Lys Ala Gly Thr Ser Trp Asn Gly Gly Tyr Gly Phe Ala Val Ala

340 345 350

Val Leu Ala Leu Thr Ala Ile Gly Leu Glu Val Arg Lys Phe Leu Lys

355 360 365

Lys

<210>95

<211>2304

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(2304)

<400>95

atg gac gtt ctc gcc tta tcc tct tcc gct tcc gcc gcc gca ccc tcc 48

Met Asp Val Leu Ala Leu Ser Ser Ser Ala Ser Ala Ala Ala Pro Ser

1 5 10 15

gct tct ctc gcc gga aaa ttc ctg tcg ttt cct tct agg gtt aga gtg 96

Ala Ser Leu Ala Gly Lys Phe Leu Ser Phe Pro Ser Arg Val Arg Val

20 25 30

aga aga aac cga gag aat ttg tta gct aaa cag aag aag ttt tta gtt 144

Arg Arg Asn Arg Glu Asn Leu Leu Ala Lys Gln Lys Lys Phe Leu Val

35 40 45

tct gct tcg aaa aga gaa gag cct aag ctc aac gaa tgg gat caa atg 192

Ser Ala Ser Lys Arg Glu Glu Pro Lys Leu Asn Glu Trp Asp Gln Met

50 55 60

gag ctc aac ttt ggc cgt tta ctc ggc gaa gac ccg aaa ttg act ttg 240

Glu Leu Asn Phe Gly Arg Leu Leu Gly Glu Asp Pro Lys Leu Thr Leu

65 70 75 80

gct aag ata gta gct aga aaa gtg gat cca gaa gct tct ttt att gac 288

Ala Lys Ile Val Ala Arg Lys Val Asp Pro Glu Ala Ser Phe Ile Asp

85 90 95

att gag aaa tct ttc tac aag aac aaa ggt aaa att cct gaa gtt gaa 336

Ile Glu Lys Ser Phe Tyr Lys Asn Lys Gly Lys Ile Pro Glu Val Glu

100 105 110

gag att cca ttg gat tgg tca aag gat aac aag aag aaa tct act agt 384

Glu Ile Pro Leu Asp Trp Ser Lys Asp Asn Lys Lys Lys Ser Thr Ser

115 120 125

tca ctg gat gga ttg aaa ttg gta aag cct gtt ctg aaa gat gga gtc 432

Ser Leu Asp Gly Leu Lys Leu Val Lys Pro Val Leu Lys Asp Gly Val

130 135 140

aag ttc gaa agg cca gtg atg aag aag cca agc cct gtt ttg aag aag 480

Lys Phe Glu Arg Pro Val Met Lys Lys Pro Ser Pro Val Leu Lys Lys

145 150 155 160

cca ttg gtg gag gct gtt gct gct cca aag gtg cag aga ttg cct aat 528

Pro Leu Val Glu Ala Val Ala Ala Pro Lys Val Gln Arg Leu Pro Asn

165 170 175

gtt ata ttg aga aag ccg agt tcg ttt tat act agt aat ggt gat gat 576

Val Ile Leu Arg Lys Pro Ser Ser Phe Tyr Thr Ser Asn Gly Asp Asp

180 185 190

gag gag tct aag ttg cgg ttg aaa ccg aat ctg aca ttg aaa atg aga 624

Glu Glu Ser Lys Leu Arg Leu Lys Pro Asn Leu Thr Leu Lys Met Arg

195 200 205

aat gag agg gaa aat gag agg ttt agt gat atg aca ttg ttg aga aaa 672

Asn Glu Arg Glu Asn Glu Arg Phe Ser Asp Met Thr Leu Leu Arg Lys

210 215 220

ccg gaa cca gtg agc gta gtt gca gaa gag gaa gac aag cct ctt tct 720

Pro Glu Pro Val Ser Val Val Ala Glu Glu Glu Asp Lys Pro Leu Ser

225 230 235 240

gat gat tta act atg gag gaa gga gaa cag gaa ggt gga aca tat tca 768

Asp Asp Leu Thr Met Glu Glu Gly Glu Gln Glu Gly Gly Thr Tyr Ser

245 250 255

cag tat act ctt ttg gag aag cca gaa gcg agg ctc cag cct gtc aat 816

Gln Tyr Thr Leu Leu Glu Lys Pro Glu Ala Arg Leu Gln Pro Val Asn

260 265 270

gta gaa gag gaa gtt gga gat agc gga gga gtg gaa tca tct gag ata 864

Val Glu Glu Glu Val Gly Asp Ser Gly Gly Val Glu Ser Ser Glu Ile

275 280 285

gta aac aac tca att cag aag cca gaa gca agg cca gag ctt gag aac 912

Val Asn Asn Ser Ile Gln Lys Pro Glu Ala Arg Pro Glu Leu Glu Asn

290 295 300

ata gaa aag gaa gtt gca gat agc gga gtt ttg gaa tca tcg gag ata 960

Ile Glu Lys Glu Val Ala Asp Ser Gly Val Leu Glu Ser Ser Glu Ile

305 310 315 320

gaa aat aat tca att cca act gaa atg cag ctc aat agc gag atg tcc 1008

Glu Asn Asn Ser Ile Pro Thr Glu Met Gln Leu Asn Ser Glu Met Ser

325 330 335

tct gag gag aaa act att aac agt gat cca ctc gag aga att cct tcg 1056

Ser Glu Glu Lys Thr Ile Asn Ser Asp Pro Leu Glu Arg Ile Pro Ser

340 345 350

aaa cca att tct caa acc atc gtc gaa gct tct tta caa ggg aaa cca 1104

Lys Pro Ile Ser Gln Thr Ile Val Glu Ala Ser Leu Gln Gly Lys Pro

355 360 365

caa aga tta gac ccg tct tcc gct gag cca tca gtt ccg aac ata gga 1152

Gln Arg Leu Asp Pro Ser Ser Ala Glu Pro Ser Val Pro Asn Ile Gly

370 375 380

aaa ccg tca gtc gtg aac cat gaa ggc cgt cag gtc tct gtt gag ctc 1200

Lys Pro Ser Val Val Asn His Glu Gly Arg Gln Val Ser Val Glu Leu

385 390 395 400

aag ggc cct cct acc aga tcg tcc ttg gag gaa aat gat tgg aat aag 1248

Lys Gly Pro Pro Thr Arg Ser Ser Leu Glu Glu Asn Asp Trp Asn Lys

405 410 415

gca gag tct cta gtt aaa aca gaa tta cga gca gat gtt gag cta ata 1296

Ala Glu Ser Leu Val Lys Thr Glu Leu Arg Ala Asp Val Glu Leu Ile

420 425 430

agt tca agc act aga gga ttt gct gtt tcc tat gga tct ttg att gga 1344

Ser Ser Ser Thr Arg Gly Phe Ala Val Ser Tyr Gly Ser Leu Ile Gly

435 440 445

ttt tta ccc tac cgg aac ctt gca gca aaa tgg aag ttt ctc gca ttt 1392

Phe Leu Pro Tyr Arg Asn Leu Ala Ala Lys Trp Lys Phe Leu Ala Phe

450 455 460

gaa tca tgg tta aga aga aaa ggt gta gat cca tca ccg tat cga caa 1440

Glu Ser Trp Leu Arg Arg Lys Gly Val Asp Pro Ser Pro Tyr Arg Gln

465 470 475 480

aac ctt ggg gta att gga ggt caa gat gtc acg agt aaa tct cca tct 1488

Asn Leu Gly Val Ile Gly Gly Gln Asp Val Thr Ser Lys Ser Pro Ser

485 490 495

cca gat tca agc tta gat tct gaa gtc gct aca acg atc aac gga gaa 1536

Pro Asp Ser Ser Leu Asp Ser Glu Val Ala Thr Thr Ile Asn Gly Glu

500 505 510

gtt tct tct gat atg aag ctg gaa gat ctt ctt atg gta tat gac aga 1584

Val Ser Ser Asp Met Lys Leu Glu Asp Leu Leu Met Val Tyr Asp Arg

515 520 525

gag aag cag aag ttc ctg tca tct ttt gtt ggt cag aaa atc aaa gtg 1632

Glu Lys Gln Lys Phe Leu Ser Ser Phe Val Gly Gln Lys Ile Lys Val

530 535 540

aat gtt gtt atg gca aat cga aat tca agg aag ctt ata ttt tca atg 1680

Asn Val Val Met Ala Asn Arg Asn Ser Arg Lys Leu Ile Phe Ser Met

545 550 555 560

agg ccg aga gaa aat gaa gag gaa gtt gag aaa aaa cga act ctt atg 1728

Arg Pro Arg Glu Asn Glu Glu Glu Val Glu Lys Lys Arg Thr Leu Met

565 570 575

gct aag ctt cgt gtt ggg gat gtt gtg aaa tgc tgc atc aag aaa att 1776

Ala Lys Leu Arg Val Gly Asp Val Val Lys Cys Cys Ile Lys Lys Ile

580 585 590

acc tat ttt ggt att ttc tgt gag cta gaa ggt gtc cct gca ttg gtt 1824

Thr Tyr Phe Gly Ile Phe Cys Glu Leu Glu Gly Val Pro Ala Leu Val

595 600 605

cac cag tca gaa gtt tca tgg gat gca act tta gac cct gct tca tat 1872

His Gln Ser Glu Val Ser Trp Asp Ala Thr Leu Asp Pro Ala Ser Tyr

610 615 620

ttc aag att ggt cag att gtg gaa gcg aaa gtg cac cag cta gat ttt 1920

Phe Lys Ile Gly Gln Ile ValGlu Ala Lys Val His Gln Leu Asp Phe

625 630 635 640

gct ctt gaa cgt atc ttc ttg tca tta aaa gaa att acg cct gat cct 1968

Ala Leu Glu Arg Ile Phe Leu Ser Leu Lys Glu Ile Thr Pro Asp Pro

645 650 655

ctt act gaa gct tta gaa tct gta gtt ggt ggt gat aat gat cag ttg 2016

Leu Thr Glu Ala Leu Glu Ser Val Val Gly Gly Asp Asn Asp Gln Leu

660 665 670

ggg gga cga tta caa gca gca gag ctc gac gct gag tgg cct gac gtg 2064

Gly Gly Arg Leu Gln Ala Ala Glu Leu Asp Ala Glu Trp Pro Asp Val

675 680 685

gaa tct ctg atc aaa gag ctg gaa atg gtt gaa gga atc caa tca gtc 2112

Glu Ser Leu Ile Lys Glu Leu Glu Met Val Glu Gly Ile Gln Ser Val

690 695 700

tca aaa agt cgt ttc ttc ttg agt ccg ggt ctt gct cca acg ttt cag 2160

Ser Lys Ser Arg Phe Phe Leu Ser Pro Gly Leu Ala Pro Thr Phe Gln

705 710 715 720

gtt tac atg gct cca atg ttt gag aac caa tac aaa ctg ctt gct cga 2208

Val Tyr Met Ala Pro Met Phe Glu Asn Gln Tyr Lys Leu Leu Ala Arg

725 730 735

gct gga aac aga gta caa gag ctt att gtt gaa gca tcc ttg agc aaa 2256

Ala Gly Asn Arg Val Gln Glu Leu Ile Val Glu Ala Ser Leu Ser Lys

740 745 750

gaa gag atg aaa tct aca atc atg tct tgc acc aac aga gta gaa tga 2304

Glu Glu Met Lys Ser Thr Ile Met Ser Cys Thr Asn Arg Val Glu

755 760 765

<210>96

<211>767

<212>PRT

＜213〉Arabidopis thaliana

<400>96

Met Asp Val Leu Ala Leu Ser Ser Ser Ala Ser Ala Ala Ala Pro Ser

1 5 10 15

Ala Ser Leu Ala Gly Lys Phe Leu Ser Phe Pro Ser Arg Val Arg Val

20 25 30

Arg Arg Asn Arg Glu Asn Leu Leu Ala Lys Gln Lys Lys Phe Leu Val

35 40 45

Ser Ala Ser Lys Arg Glu Glu Pro Lys Leu Asn Glu Trp Asp Gln Met

50 55 60

Glu Leu Asn Phe Gly Arg Leu Leu Gly Glu Asp Pro Lys Leu Thr Leu

65 70 75 80

Ala Lys Ile Val Ala Arg Lys Val Asp Pro Glu Ala Ser Phe Ile Asp

85 90 95

Ile Glu Lys Ser Phe Tyr Lys Asn Lys Gly Lys Ile Pro Glu Val Glu

100 105 110

Glu Ile Pro Leu Asp Trp Ser Lys Asp Asn Lys Lys Lys Ser Thr Ser

115 120 125

Ser Leu Asp Gly Leu Lys Leu Val Lys Pro Val Leu Lys Asp Gly Val

130 135 140

Lys Phe Glu Arg Pro Val Met Lys Lys Pro Ser Pro Val Leu Lys Lys

145 150 155 160

Pro Leu Val Glu Ala Val Ala Ala Pro Lys Val Gln Arg Leu Pro Asn

165 170 175

Val Ile Leu Arg Lys Pro Ser Ser Phe Tyr Thr Ser Asn Gly Asp Asp

180 185 190

Glu Glu Ser Lys Leu Arg Leu Lys Pro Asn Leu Thr Leu Lys Met Arg

195 200 205

Asn Glu Arg Glu Asn Glu Arg Phe Ser Asp Met Thr Leu Leu Arg Lys

210 215 220

Pro Glu Pro Val Ser Val Val Ala Glu Glu Glu Asp Lys Pro Leu Ser

225 230 235 240

Asp Asp Leu Thr Met Glu Glu Gly Glu Gln Glu Gly Gly Thr Tyr Ser

245 250 255

Gln Tyr Thr Leu Leu Glu Lys Pro Glu Ala Arg Leu Gln Pro Val Asn

260 265 270

Val Glu Glu Glu Val Gly Asp Ser Gly Gly Val Glu Ser Ser Glu Ile

275 280 285

Val Asn Asn Ser Ile Gln Lys Pro Glu Ala Arg Pro Glu Leu Glu Asn

290 295 300

Ile Glu Lys Glu Val Ala Asp Ser Gly Val Leu Glu Ser Ser Glu Ile

305 310 315 320

Glu Asn Asn Ser Ile Pro Thr Glu Met Gln Leu Asn Ser Glu Met Ser

325 330 335

Ser Glu Glu Lys Thr Ile Asn Ser Asp Pro Leu Glu Arg Ile Pro Ser

340 345 350

Lys Pro Ile Ser Gln Thr Ile Val Glu Ala Ser Leu Gln Gly Lys Pro

355 360 365

Gln Arg Leu Asp Pro Ser Ser Ala Glu Pro Ser Val Pro Asn Ile Gly

370 375 380

Lys Pro Ser Val Val Asn His Glu Gly Arg Gln Val Ser Val Glu Leu

385 390 395 400

Lys Gly Pro Pro Thr Arg Ser Ser Leu Glu Glu Asn Asp Trp Asn Lys

405 410 415

Ala Glu Ser Leu Val Lys Thr Glu Leu Arg Ala Asp Val Glu Leu Ile

420 425 430

Ser Ser Ser Thr Arg Gly Phe Ala Val Ser Tyr Gly Ser Leu Ile Gly

435 440 445

Phe Leu Pro Tyr Arg Asn Leu Ala Ala Lys Trp Lys Phe Leu Ala Phe

450 455 460

Glu Ser Trp Leu Arg Arg Lys Gly Val Asp Pro Ser Pro Tyr Arg Gln

465 470 475 480

Asn Leu Gly Val Ile Gly Gly Gln Asp Val Thr Ser Lys Ser Pro Ser

485 490 495

Pro Asp Ser Ser Leu Asp Ser Glu Val Ala Thr Thr Ile Asn Gly Glu

500 505 510

Val Ser Ser Asp Met Lys Leu Glu Asp Leu Leu Met Val Tyr Asp Arg

515 520 525

Glu Lys Gln Lys Phe Leu Ser Ser Phe Val Gly Gln Lys Ile Lys Val

530 535 540

Asn Val Val Met Ala Asn Arg Asn Ser Arg Lys Leu Ile Phe Ser Met

545 550 555 560

Arg Pro Arg Glu Asn Glu Glu Glu Val Glu Lys Lys Arg Thr Leu Met

565 570 575

Ala Lys Leu Arg Val Gly Asp Val Val Lys Cys Cys Ile Lys Lys Ile

580 585 590

Thr Tyr Phe Gly Ile Phe Cys Glu Leu Glu Gly Val Pro Ala Leu Val

595 600 605

His Gln Ser Glu Val Ser Trp Asp Ala Thr Leu Asp Pro Ala Ser Tyr

610 615 620

Phe Lys Ile Gly Gln Ile Val Glu Ala Lys Val His Gln Leu Asp Phe

625 630 635 640

Ala Leu Glu Arg Ile Phe Leu Ser Leu Lys Glu Ile Thr Pro Asp Pro

645 650 655

Leu Thr Glu Ala Leu Glu Ser Val Val Gly Gly Asp Asn Asp Gln Leu

660 665 670

Gly Gly Arg Leu Gln Ala Ala Glu Leu Asp Ala Glu Trp Pro Asp Val

675 680 685

Glu Ser Leu Ile Lys Glu Leu Glu Met Val Glu Gly Ile Gln Ser Val

690 695 700

Ser Lys Ser Arg Phe Phe Leu Ser Pro Gly Leu Ala Pro Thr Phe Gln

705 710 715 720

Val Tyr Met Ala Pro Met Phe Glu Asn Gln Tyr Lys Leu Leu Ala Arg

725 730 735

Ala Gly Asn Arg Val Gln Glu Leu Ile Val Glu Ala Ser Leu Ser Lys

740 745 750

Glu Glu Met Lys Ser Thr Ile Met Ser Cys Thr Asn Arg Val Glu

755 760 765

<210>97

<211>2703

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(2703)

<400>97

atg ggg tca gac gag gaa gat ttc gtg ttt cat gga acg cca ata gag 48

Met Gly Ser Asp Glu Glu Asp Phe Val Phe His Gly Thr Pro Ile Glu

1 5 10 15

cgc gaa gaa gaa atc gca agc cgg aag aag aaa gca gtc gct ggg gct 96

Arg Glu Glu Glu Ile Ala Ser Arg Lys Lys Lys Ala Val Ala Gly Ala

20 25 30

tct ggc aat ctt aga act ctc cct gct tgg aag caa gag gtg act gat 144

Ser Gly Asn Leu Arg Thr Leu Pro Ala Trp Lys Gln Glu Val Thr Asp

35 40 45

gaa gaa ggc cgt aga agg ttc cat gga gca ttt act ggt gga tat tct 192

Glu Glu Gly Arg Arg Arg Phe His Gly Ala Phe Thr Gly Gly Tyr Ser

50 55 60

gct ggg tat tac aat aca gtt gga tca aaa gag ggc tgg gct cca cag 240

Ala Gly Tyr Tyr Asn Thr Val Gly Ser Lys Glu Gly Trp Ala Pro Gln

65 70 75 80

tca ttt aca tca tca agg cag aac aga gct gga gcg aga aag caa agt 288

Ser Phe Thr Ser Ser Arg Gln Asn Arg Ala Gly Ala Arg Lys Gln Ser

85 90 95

att tca gac ttt cta gat gaa gat gaa aag gcg gat atg gag ggc aaa 336

Ile Ser Asp Phe Leu Asp Glu Asp Glu Lys Ala Asp Met Glu Gly Lys

100 105 110

tca ctg tct gcg agc tca caa ttt gac aca ttt ggg ttt acg gca gcc 384

Ser Leu Ser Ala Ser Ser Gln Phe Asp Thr Phe Gly Phe Thr Ala Ala

115 120 125

gaa cat tcc cgc aag cat gct gag aaa gaa cag cat gag agg cca tca 432

Glu His Ser Arg Lys His Ala Glu Lys Glu Gln His Glu Arg Pro Ser

130 135 140

gcc att cct ggc cct gtt cct gac gaa ctt gtt gct cca gtt tca gag 480

Ala Ile Pro Gly Pro Val Pro Asp Glu Leu Val Ala Pro Val Ser Glu

145 150 155 160

tca att ggg gtc aaa ctt ttg cta aag atg gga tgg cgg cgt ggt cat 528

Ser Ile Gly Val Lys Leu Leu Leu Lys Met Gly Trp Arg Arg Gly His

165 170 175

tca ata aag gaa gtg cgt gcc agt tca gat gct cgt aga gaa gct aga 576

Ser Ile Lys Glu Val Arg Ala Ser Ser Asp Ala Arg Arg Glu Ala Arg

180 185 190

aaa gca ttc tta gcc ttc tat act gat gag aat aca aag gaa acg ccc 624

Lys Ala Phe Leu Ala Phe Tyr Thr Asp Glu Asn Thr Lys Glu Thr Pro

195 200 205

gac tcg ctt gtt tct gag act gaa gtg gaa act tct ctg ggt gaa gat 672

Asp Ser Leu Val Ser Glu Thr Glu Val Glu Thr Ser Leu Gly Glu Asp

210 215 220

att aaa att tct gaa agc act cct gta tat gtt ctg aat cca aag caa 720

Ile Lys Ile Ser Glu Ser Thr Pro Val Tyr Val Leu Asn Pro Lys Gln

225 230 235 240

gat ctg cat gga tta gga tat gat cct ttt aag cat gct cct gaa ttt 768

Asp Leu His Gly Leu Gly Tyr Asp Pro Phe Lys His Ala Pro Glu Phe

245 250 255

aga gga aag att gct ccg ggt ttt ggc att gga gca ctt gag gaa ctt 816

Arg Gly Lys Ile Ala Pro Gly Phe Gly Ile Gly Ala Leu Glu Glu Leu

260 265 270

gat gtt gag gat gaa gat gtc tat gct ggt tac gat ttt gat cag act 864

Asp Val Glu Asp Glu Asp Val Tyr Ala Gly Tyr Asp Phe Asp Gln Thr

275 280 285

tat gtc ata gaa gac gaa cag cca gca aga cag agc aat gac aat aga 912

Tyr Val Ile Glu Asp Glu Gln Pro Ala Arg Gln Ser Asn Asp Asn Arg

290 295 300

ctg agg tta acc tca aaa gag cat gac gtt ctg cca ggt ttt gga gct 960

Leu Arg Leu Thr Ser Lys Glu His Asp Val Leu Pro Gly Phe Gly Ala

305 310 315 320

gct aag aat tct gac tac agt atg gag aga ttt aat cct ccg ata atc 1008

Ala Lys Asn Ser Asp Tyr Ser Met Glu Arg Phe Asn Pro Pro Ile Ile

325 330 335

ccg aag gat ttt gtg gcc cgg cat aaa ttt tct ggt cct ctt gag gct 1056

Pro Lys Asp Phe Val Ala Arg His Lys Phe Ser Gly Pro Leu Glu Ala

340 345 350

gaa act aag cca act gtt tct gct cct ccg gaa gtt cct cct cct gca 1104

Glu Thr Lys Pro Thr Val Ser Ala Pro Pro Glu Val Pro Pro Pro Ala

355 360 365

gat aat aat ctg aaa ctt ctg atc gag ggg ttt gca act ttt gtt tcc 1152

Asp Asn Asn Leu Lys Leu Leu Ile Glu Gly Phe Ala Thr Phe Val Ser

370 375 380

cgt tgc ggg aaa cta tac gag gat ctt tct aga gag aag aac caa tca 1200

Arg Cys Gly Lys Leu Tyr Glu Asp Leu Ser Arg Glu Lys Asn Gln Ser

385 390 395 400

aat cag ctg ttt gat ttt ctt cgg gaa ggt aac ggt cat gac tac tac 1248

Asn Gln Leu Phe Asp Phe Leu Arg Glu Gly Asn Gly His Asp Tyr Tyr

405 410 415

gca aga agg ctg tgg gag gag cag caa aag cgt aaa gat caa agt aag 1296

Ala Arg Arg Leu Trp Glu Glu Gln Gln Lys Arg Lys Asp Gln Ser Lys

420 425 430

ctg aca tta gat gtt aag gtg tct cca acc gta cag aaa atg act gca 1344

Leu Thr Leu Asp Val Lys Val Ser Pro Thr Val Gln Lys Met Thr Ala

435 440 445

gaa aca cgt ggc agc tta tta ggg gaa aag cca ttg cag aga agt ttg 1392

Glu Thr Arg Gly Ser Leu Leu Gly Glu Lys Pro Leu Gln Arg Ser Leu

450 455 460

aaa gaa acc gat act tct gct tct tct gga ggc tcc ttc cag ttc ccg 1440

Lys Glu Thr Asp Thr Ser Ala Ser Ser Gly Gly Ser Phe Gln Phe Pro

465 470 475 480

acc aat ctc tct gac aca ttc acc aaa tca gct tca tct caa gag gca 1488

Thr Asn Leu Ser Asp Thr Phe Thr Lys Ser Ala Ser Ser Gln Glu Ala

485 490 495

gca gat gct gtg aag ccc ttc aaa gat gat cca gct aaa caa gaa aga 1536

Ala Asp Ala Val Lys Pro Phe Lys Asp Asp Pro Ala Lys Gln Glu Arg

500 505 510

ttt gag cag ttt ctc aag gag aaa tac aaa gga ggg tta cgt aca aca 1584

Phe Glu Gln Phe Leu Lys Glu Lys Tyr Lys Gly Gly Leu Arg Thr Thr

515 520 525

gac tcc aac aga gtt aat agc atg tcg gaa tca gct cgg gca caa gag 1632

Asp Ser Asn Arg Val Asn Ser Met Ser Glu Ser Ala Arg Ala Gln Glu

530 535 540

agg ctg gac ttt gag gct gca gcc gag gca att gag aaa ggg aaa gct 1680

Arg Leu Asp Phe Glu Ala Ala Ala Glu Ala Ile Glu Lys Gly Lys Ala

545 550 555 560

tac aag gag gtc aga cgg gct acc gaa cag cct ctc gat ttc ctt gct 1728

Tyr Lys Glu Val Arg Arg Ala Thr Glu Gln Pro Leu Asp Phe Leu Ala

565 570 575

gga ggt ctt cag ttt act tct ggg gga aca gag caa att aaa gac act 1776

Gly Gly Leu Gln Phe Thr Ser Gly Gly Thr Glu Gln Ile Lys Asp Thr

580 585 590

gga gtg gta gac atg aaa tcg agt aag acg tat cct aaa agg gaa gag 1824

Gly Val Val Asp Met Lys Ser Ser Lys Thr Tyr Pro Lys Arg Glu Glu

595 600 605

ttc caa tgg cgt cct tca cct ctt ttg tgc aaa cgt ttt gat ctc ccc 1872

Phe Gln Trp Arg Pro Ser Pro Leu Leu Cys Lys Arg Phe Asp Leu Pro

610 615 620

gat cca ttc atg gga aag ctg cca cct gct ccg cga gcg aga aac aaa 1920

Asp Pro Phe Met Gly Lys Leu Pro Pro Ala Pro Arg Ala Arg Asn Lys

625 630 635 640

atg gat tct ctc gta ttc ttg ccg gat aca gtg aaa gct gca tct gca 1968

Met Asp Ser Leu Val Phe Leu Pro Asp Thr Val Lys Ala Ala Ser Ala

645 650 655

cgt caa gta tct gag tcg caa gta cct aag aaa gag aca tca ata gaa 2016

Arg Gln Val Ser Glu Ser Gln Val Pro Lys Lys Glu Thr Ser Ile Glu

660 665 670

gag cct gaa gtt gag gta gaa gtg gag aat gtg gag aga cct gtt gat 2064

Glu Pro Glu Val Glu Val Glu Val Glu Asn Val Glu Arg Pro Val Asp

675 680 685

ctt tac aag gct atc ttc tct gat gat tct gaa gat gat gaa gat caa 2112

Leu Tyr Lys Ala Ile Phe Ser Asp Asp Ser Glu Asp Asp Glu Asp Gln

690 695 700

cca atg aat gga aag ata caa gag ggt caa gaa aag aag aat gaa gcg 2160

Pro Met Asn Gly Lys Ile Gln Glu Gly Gln Glu Lys Lys Asn Glu Ala

705 710 715 720

gct gca acc aca tta aac cgg ctt ata gct ggc gat ttc cta gaa tct 2208

Ala Ala Thr Thr Leu Asn Arg Leu Ile Ala Gly Asp Phe Leu Glu Ser

725 730 735

tta ggg aaa gaa cta ggg ttc gag gtg cca atg gaa gaa gag atc aag 2256

Leu Gly Lys Glu Leu Gly Phe Glu Val Pro Met Glu Glu Glu Ile Lys

740 745 750

tcc aga agc aaa ccc gaa gat tct tct gat aaa aga ctt gat cga ccc 2304

Ser Arg Ser Lys Pro Glu Asp Ser Ser Asp Lys Arg Leu Asp Arg Pro

755 760 765

gga ttg aaa gag aaa gtg gag gag aag aca agc agc ctc aca ctt ggg 2352

Gly Leu Lys Glu Lys Val Glu Glu Lys Thr Ser Ser Leu Thr Leu Gly

770 775 780

tct gaa gaa gaa aag agt aga aaa aag aga gag aaa tcg cca gga aaa 2400

Ser Glu Glu Glu Lys Ser Arg Lys Lys Arg Glu Lys Ser Pro Gly Lys

785 790 795 800

cgg agt ggt ggc aac gat cta tca tcg agt gaa tcc tca gga gat gaa 2448

Arg Ser Gly Gly Asn Asp Leu Ser Ser Ser Glu Ser Ser Gly Asp Glu

805 810 815

cgg agg aga aaa cga tat aat aag aag gat aga cat aga aac gat tca 2496

Arg Arg Arg Lys Arg Tyr Asn Lys Lys Asp Arg His Arg Asn Asp Ser

820 825 830

gag agc gat tca tcc agc gac tac cac agc agg gat aag caa gga tca 2544

Glu Ser Asp Ser Ser Ser Asp Tyr His Ser Arg Asp Lys Gln Gly Ser

835 840 845

aga tct agg agc aag cgg aga gaa tct tct aga gag aag aga agt agc 2592

Arg Ser Arg Ser Lys Arg Arg Glu Ser Ser Arg Glu Lys Arg Ser Ser

850 855 860

cac aag aag cac tca aag cat cgc agg acc aag aag tct tct tct tca 2640

His Lys Lys His Ser Lys His Arg Arg Thr Lys Lys Ser Ser Ser Ser

865 870 875 880

cgg tat agc tca gac gaa gaa caa aaa gag tca agg cgg gag aag aag 2688

Arg Tyr Ser Ser Asp Glu Glu Gln Lys Glu Ser Arg Arg Glu Lys Lys

885 890 895

agg cga cga gac tga 2703

Arg Arg Arg Asp

900

<210>98

<211>900

<212>PRT

＜213〉Arabidopis thaliana

<400>98

Met Gly Ser Asp Glu Glu Asp Phe Val Phe His Gly Thr Pro Ile Glu

1 5 10 15

Arg Glu Glu Glu Ile Ala Ser Arg Lys Lys Lys Ala Val Ala Gly Ala

20 25 30

Ser Gly Asn Leu Arg Thr Leu Pro Ala Trp Lys Gln Glu Val Thr Asp

35 40 45

Glu Glu Gly Arg Arg Arg Phe His Gly Ala Phe Thr Gly Gly Tyr Ser

50 55 60

Ala Gly Tyr Tyr Asn Thr Val Gly Ser Lys Glu Gly Trp Ala Pro Gln

65 70 75 80

Ser Phe Thr Ser Ser Arg Gln Asn Arg Ala Gly Ala Arg Lys Gln Ser

85 90 95

Ile Ser Asp Phe Leu Asp Glu Asp Glu Lys Ala Asp Met Glu Gly Lys

100 105 110

Ser Leu Ser Ala Ser Ser Gln Phe Asp Thr Phe Gly Phe Thr Ala Ala

115 120 125

Glu His Ser Arg Lys His Ala Glu Lys Glu Gln His Glu Arg Pro Ser

130 135 140

Ala Ile Pro Gly Pro Val Pro Asp Glu Leu Val Ala Pro Val Ser Glu

145 150 155 160

Ser Ile Gly Val Lys Leu Leu Leu Lys Met Gly Trp Arg Arg Gly His

165 170 175

Ser Ile Lys Glu Val Arg Ala Ser Ser Asp Ala Arg Arg Glu Ala Arg

180 185 190

Lys Ala Phe Leu Ala Phe Tyr Thr Asp Glu Asn Thr Lys Glu Thr Pro

195 200 205

Asp Ser Leu Val Ser Glu Thr Glu Val Glu Thr Ser Leu Gly Glu Asp

210 215 220

Ile Lys Ile Ser Glu Ser Thr Pro Val Tyr Val Leu Asn Pro Lys Gln

225 230 235 240

Asp Leu His Gly Leu Gly Tyr Asp Pro Phe Lys His Ala Pro Glu Phe

245 250 255

Arg Gly Lys Ile Ala Pro Gly Phe Gly Ile Gly Ala Leu Glu Glu Leu

260 265 270

Asp Val Glu Asp Glu Asp Val Tyr Ala Gly Tyr Asp Phe Asp Gln Thr

275 280 285

Tyr Val Ile Glu Asp Glu Gln Pro Ala Arg Gln Ser Asn Asp Asn Arg

290 295 300

Leu Arg Leu Thr Ser Lys Glu His Asp Val Leu Pro Gly Phe Gly Ala

305 310 315 320

Ala Lys Asn Ser Asp Tyr Ser Met Glu Arg Phe Asn Pro Pro Ile Ile

325 330 335

Pro Lys Asp Phe Val Ala Arg His Lys Phe Ser Gly Pro Leu Glu Ala

340 345 350

Glu Thr Lys Pro Thr Val Ser Ala Pro Pro Glu Val Pro Pro Pro Ala

355 360 365

Asp Asn Asn Leu Lys Leu Leu Ile Glu Gly Phe Ala Thr Phe Val Ser

370 375 380

Arg Cys Gly Lys Leu Tyr Glu Asp Leu Ser Arg Glu Lys Asn Gln Ser

385 390 395 400

Asn Gln Leu Phe Asp Phe Leu Arg Glu Gly Asn Gly His Asp Tyr Tyr

405 410 415

Ala Arg Arg Leu Trp Glu Glu Gln Gln Lys Arg Lys Asp Gln Ser Lys

420 425 430

Leu Thr Leu Asp Val Lys Val Ser Pro Thr Val Gln Lys Met Thr Ala

435 440 445

Glu Thr Arg Gly Ser Leu Leu Gly Glu Lys Pro Leu Gln Arg Ser Leu

450 455 460

Lys Glu Thr Asp Thr Ser Ala Ser Ser Gly Gly Ser Phe Gln Phe Pro

465 470 475 480

Thr Asn Leu Ser Asp Thr Phe Thr Lys Ser Ala Ser Ser Gln Glu Ala

485 490 495

Ala Asp Ala Val Lys Pro Phe Lys Asp Asp Pro Ala Lys Gln Glu Arg

500 505 510

Phe Glu Gln Phe Leu Lys Glu Lys Tyr Lys Gly Gly Leu Arg Thr Thr

515 520 525

Asp Ser Asn Arg Val Asn Ser Met Ser Glu Ser Ala Arg Ala Gln Glu

530 535 540

Arg Leu Asp Phe Glu Ala Ala Ala Glu Ala Ile Glu Lys Gly Lys Ala

545 550 555 560

Tyr Lys Glu Val Arg Arg Ala Thr Glu Gln Pro Leu Asp Phe Leu Ala

565 570 575

Gly Gly Leu Gln Phe Thr Ser Gly Gly Thr Glu Gln Ile Lys Asp Thr

580 585 590

Gly Val Val Asp Met Lys Ser Ser Lys Thr Tyr Pro Lys Arg Glu Glu

595 600 605

Phe Gln Trp Arg Pro Ser Pro Leu Leu Cys Lys Arg Phe Asp Leu Pro

610 615 620

Asp Pro Phe Met Gly Lys Leu Pro Pro Ala Pro Arg Ala Arg Asn Lys

625 630 635 640

Met Asp Ser Leu Val Phe Leu Pro Asp Thr Val Lys Ala Ala Ser Ala

645 650 655

Arg Gln Val Ser Glu Ser Gln Val Pro Lys Lys Glu Thr Ser Ile Glu

660 665 670

Glu Pro Glu Val Glu Val Glu Val Glu Asn Val Glu Arg Pro Val Asp

675 680 685

Leu Tyr Lys Ala Ile Phe Ser Asp Asp Ser Glu Asp Asp Glu Asp Gln

690 695 700

Pro Met Asn Gly Lys Ile Gln Glu Gly Gln Glu Lys Lys Asn Glu Ala

705 710 715 720

Ala Ala Thr Thr Leu Asn Arg Leu Ile Ala Gly Asp Phe Leu Glu Ser

725 730 735

Leu Gly Lys Glu Leu Gly Phe Glu Val Pro Met Glu Glu Glu Ile Lys

740 745 750

Ser Arg Ser Lys Pro Glu Asp Ser Ser Asp Lys Arg Leu Asp Arg Pro

755 760 765

Gly Leu Lys Glu Lys Val Glu Glu Lys Thr Ser Ser Leu Thr Leu Gly

770 775 780

Ser Glu Glu Glu Lys Ser Arg Lys Lys Arg Glu Lys Ser Pro Gly Lys

785 790 795 800

Arg Ser Gly Gly Asn Asp Leu Ser Ser Ser Glu Ser Ser Gly Asp Glu

805 810 815

Arg Arg Arg Lys Arg Tyr Asn Lys Lys Asp Arg His Arg Asn Asp Ser

820 825 830

Glu Ser Asp Ser Ser Ser Asp Tyr His Ser Arg Asp Lys Gln Gly Ser

835 840 845

Arg Ser Arg Ser Lys Arg Arg Glu Ser Ser Arg Glu Lys Arg Ser Ser

850 855 860

His Lys Lys His Ser Lys His Arg Arg Thr Lys Lys Ser Ser Ser Ser

865 870 875 880

Arg Tyr Ser Ser Asp Glu Glu Gln Lys Glu Ser Arg Arg Glu Lys Lys

885 890 895

Arg Arg Arg Asp

900

<210>99

<211>1074

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(1074)

<400>99

atg tcg tcc ccg gaa aag ttt tcg cca gcg ccg gaa tcg aac tca aat 48

Met Ser Ser Pro Glu Lys Phe Ser Pro Ala Pro Glu Ser Asn Ser Asn

1 5 10 15

ccg tca ctt ccc gat gct ttg ata ata agc tgc atc gca cga gtc tca 96

Pro Ser Leu Pro Asp Ala Leu Ile Ile Ser Cys Ile Ala Arg Val Ser

20 25 30

aga ttg tat tat ccg att ctc tcc ttt gtc tcc aag agc ttt cga tct 144

Arg Leu Tyr Tyr Pro Ile Leu Ser Phe Val Ser Lys Ser Phe Arg Ser

35 40 45

ctc cta gct tca ccg gag ctt tac aag gaa cgg tca ctc ttg aac cgc 192

Leu Leu Ala Ser Pro Glu Leu Tyr Lys Glu Arg Ser Leu Leu Asn Arg

50 55 60

acc gag ggt tgt cta tat gta tgc tta tac tta aat cct ttt gag agc 240

Thr Glu Gly Cys Leu Tyr Val Cys Leu Tyr Leu Asn Pro Phe Glu Ser

65 70 75 80

cct agc tgg ttt act ctc tgc ttg aaa cct gat caa gcc cta tct tct 288

Pro Ser Trp Phe Thr Leu Cys Leu Lys Pro Asp Gln Ala Leu Ser Ser

85 90 95

gaa aca agt aat aag aag aag tca agt ggg tat gtt ttg gct aca gta 336

Glu Thr Ser Asn Lys Lys Lys Ser Ser Gly Tyr Val Leu Ala Thr Val

100 105 110

tca att cca cat cct cgt ctt gtg caa cgt tcc agt ctc gtg gcg gtt 384

Ser Ile Pro His Pro Arg Leu Val Gln Arg Ser Ser Leu Val Ala Val

115 120 125

ggt tct aat atc tac aac att ggc aga tcc ata tca cct tac tct agt 432

Gly Ser Asn Ile Tyr Asn Ile Gly Arg Ser Ile Ser Pro Tyr Ser Ser

130 135 140

gtc tcg att ttt gat tgc cgg tct cac acg tgg cgc gag gct cca agc 480

Val Ser Ile Phe Asp Cys Arg Ser His Thr Trp Arg Glu Ala Pro Ser

145 150 155 160

ttg cca gtg gag cta gtt gaa gtt tct gct ggc gtc ctt gac gga aag 528

Leu Pro Val Glu Leu Val Glu Val Ser Ala Gly Val Leu Asp Gly Lys

165 170 175

ata tat gta gcc gga agt tgc aaa gat gga gat tct ctt aac ttg aag 576

Ile Tyr Val Ala Gly Ser Cys Lys Asp Gly Asp Ser Leu Asn Leu Lys

180 185 190

aac act ttc gag gtg ttc gac aca aaa aca caa gtt tgg gat cat gta 624

Asn Thr Phe Glu Val Phe Asp Thr Lys Thr Gln Val Trp Asp His Val

195 200 205

cct atc cct tac aac gaa aca aaa cac aac att tac tcc aaa agc tta 672

Pro Ile Pro Tyr Asn Glu Thr Lys His Asn lle Tyr Ser Lys Ser Leu

210 215 220

tgt att gac gaa aag tgg tat gta ggg gct aag aga aag gtg gtt tct 720

Cys Ile Asp Glu Lys Trp Tyr Val Gly Ala Lys Arg Lys Val Val Ser

225 230 235 240

tac aat ccc aag aaa ggt ata tgg gac ctt gtt gaa tca gag atg tgt 768

Tyr Asn Pro Lys Lys Gly Ile Trp Asp Leu Val Glu Ser Glu Met Cys

245 250 255

agt tat aag tct tca tat gat tat tgt gag ata gag aac gtt ttg tac 816

Ser Tyr Lys Ser Ser Tyr Asp Tyr Cys Glu Ile Glu Asn Val Leu Tyr

260 265 270

tct gtc gaa aaa aca tgg cgt ggc act gtt ttc aga tgg tat gac act 864

Ser Val Glu Lys Thr Trp Arg Gly Thr Val Phe Arg Trp Tyr Asp Thr

275 280 285

gag cta gga cgg tgg aga aag ttg gag ggt ttg aat atg cct tat agt 912

Glu Leu Gly Arg Trp Arg Lys Leu Glu Gly Leu Asn Met Pro Tyr Ser

290 295 300

ggg act ggt gac aga ggc ggt aag aag atg att tgg tgt gcg gtg att 960

Gly Thr Gly Asp Arg Gly Gly Lys Lys Met Ile Trp Cys Ala Val Ile

305 310 315 320

acg ctt gaa agg cgc aaa aat agt gga att tgg gga aac gtt gag tgg 1008

Thr Leu Glu Arg Arg Lys Asn Ser Gly Ile Trp Gly Asn Val Glu Trp

325 330 335

ttt gct cat gtg ctt aca gtt cct aaa aca ttt gtt ttc caa aag ttt 1056

Phe Ala His Val Leu Thr Val Pro Lys Thr Phe Val Phe Gln Lys Phe

340 345 350

ctt gct gct act gtc taa 1074

Leu Ala Ala Thr Val

355

<210>100

<211>357

<212>PRT

＜213〉Arabidopis thaliana

<400>100

Met Ser Ser Pro Glu Lys Phe Ser Pro Ala Pro Glu Ser Asn Ser Asn

1 5 10 15

Pro Ser Leu Pro Asp Ala Leu Ile Ile Ser Cys lle Ala Arg Val Ser

20 25 30

Arg Leu Tyr Tyr Pro Ile Leu Ser Phe Val Ser Lys Ser Phe Arg Ser

35 40 45

Leu Leu Ala Ser Pro Glu Leu Tyr Lys Glu Arg Ser Leu Leu Asn Arg

50 55 60

Thr Glu Gly Cys Leu Tyr Val Cys Leu Tyr Leu Asn Pro Phe Glu Ser

65 70 75 80

Pro Ser Trp Phe Thr Leu Cys Leu Lys Pro Asp Gln Ala Leu Ser Ser

85 90 95

Glu Thr Ser Asn Lys Lys Lys Ser Ser Gly Tyr Val Leu Ala Thr Val

100 105 110

Ser Ile Pro His Pro Arg Leu Val Gln Arg Ser Ser Leu Val Ala Val

115 120 125

Gly Ser Asn Ile Tyr Asn Ile Gly Arg Ser Ile Ser Pro Tyr Ser Ser

130 135 140

Val Ser Ile Phe Asp Cys Arg Ser His Thr Trp Arg Glu Ala Pro Ser

145 150 155 160

Leu Pro Val Glu Leu Val Glu Val Ser Ala Gly Val Leu Asp Gly Lys

165 170 175

Ile Tyr Val Ala Gly Ser Cys Lys Asp Gly Asp Ser Leu Asn Leu Lys

180 185 190

Asn Thr Phe Glu ValPhe Asp Thr Lys Thr Gln Val Trp Asp His Val

195 200 205

Pro Ile Pro Tyr Asn Glu Thr Lys His Asn Ile Tyr Ser Lys Ser Leu

210 215 220

Cys Ile Asp Glu Lys Trp Tyr Val Gly Ala Lys Arg Lys Val Val Ser

225 230 235 240

Tyr Asn Pro Lys Lys Gly Ile Trp Asp Leu Val Glu Ser Glu Met Cys

245 250 255

Ser Tyr Lys Ser Ser Tyr Asp Tyr Cys Glu Ile Glu Asn Val Leu Tyr

260 265 270

Ser Val Glu Lys Thr Trp Arg Gly Thr Val Phe Arg Trp Tyr Asp Thr

275 280 285

Glu Leu Gly Arg Trp Arg Lys Leu Glu Gly Leu Asn Met Pro Tyr Ser

290 295 300

Gly Thr Gly Asp Arg Gly Gly Lys Lys Met Ile Trp Cys Ala Val Ile

305 310 315 320

Thr Leu Glu Arg Arg Lys Asn Ser Gly Ile Trp Gly Asn Val Glu Trp

325 330 335

Phe Ala His Val Leu Thr Val Pro Lys Thr Phe Val Phe Gln Lys Phe

340 345 350

Leu Ala Ala Thr Val

355

<210>101

<211>2520

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(2520)

<400>101

atg gtt aaa gta agg aag atg gag atg att tta tta cta att ctt gtg 48

Met Val Lys Val Arg Lys Met Glu Met Ile Leu Leu Leu Ile Leu Val

1 5 10 15

att gtg gtg gcg gcg acg gcg gcg aat gtg act tat gac cac cgt gca 96

Ile Val Val Ala Ala Thr Ala Ala Asn Val Thr Tyr Asp His Arg Ala

20 25 30

tta gta atc gac ggg aaa cgg aaa gtt cta atc tct ggt tct att cat 144

Leu Val Ile Asp Gly Lys Arg Lys Val Leu Ile Ser Gly Ser Ile His

35 40 45

tat cct cgg agt act cct gag atg tgg cca gag ctt ata cag aaa tct 192

Tyr Pro Arg Ser Thr Pro Glu Met Trp Pro Glu Leu Ile Gln Lys Ser

50 55 60

aaa gac ggt ggt tta gat gtt ata gag acg tat gtg ttt tgg agt ggt 240

Lys Asp Gly Gly Leu Asp Val Ile Glu Thr Tyr Val Phe Trp Ser Gly

65 70 75 80

cac gaa ccg gag aaa aat aag tat aat ttt gaa gga aga tat gat tta 288

His Glu Pro Glu Lys Asn Lys Tyr Asn Phe Glu Gly Arg Tyr Asp Leu

85 90 95

gtg aaa ttt gtg aag ctt gcg gct aaa gct ggt ctc tat gtt cat tta 336

Val Lys Phe Val Lys Leu Ala Ala Lys Ala Gly Leu Tyr Val His Leu

100 105 110

aga att ggt cct tac gtc tgt gct gaa tgg aat tac ggt ggt ttc cca 384

Arg Ile Gly Pro Tyr Val Cys Ala Glu Trp Asn Tyr Gly Gly Phe Pro

115 120 125

gtg tgg ttg cat ttt gtt cca gga att aag ttt cga act gat aat gag 432

Val Trp Leu His Phe Val Pro Gly Ile Lys Phe Arg Thr Asp Asn Glu

130 135 140

cca ttt aag gaa gaa atg cag aga ttt acc aca aag att gtt gat ttg 480

Pro Phe Lys Glu Glu Met Gln Arg Phe Thr Thr Lys Ile Val Asp Leu

145 150 155 160

atg aag caa gaa aag ctt tat gca tca caa gga ggt cca atc att ctc 528

Met Lys Gln Glu Lys Leu Tyr Ala Ser Gln Gly Gly Pro Ile Ile Leu

165 170 175

tcg cag att gag aat gaa tat gga aat att gac tca gct tat ggt gcg 576

Ser Gln Ile Glu Asn Glu Tyr Gly Asn Ile Asp Ser Ala Tyr Gly Ala

180 185 190

gct gct aaa agt tat atc aag tgg tct gct tct atg gct ctt tcg tta 624

Ala Ala Lys Ser Tyr Ile Lys Trp Ser Ala Ser Met Ala Leu Ser Leu

195 200 205

gat act gga gta cca tgg aat atg tgt caa caa aca gat gct cct gat 672

Asp Thr Gly Val Pro Trp Asn Met Cys Gln Gln Thr Asp Ala Pro Asp

210 215 220

ccc atg atc aac aca tgc aat ggt ttc tac tgt gac cag ttt aca cct 720

Pro Met Ile Asn Thr Cys Asn Gly Phe Tyr Cys Asp Gln Phe Thr Pro

225 230 235 240

aac tca aat aat aaa cca aag atg tgg acc gag aac tgg agt gga tgg 768

Asn Ser Asn Asn Lys Pro Lys Met Trp Thr Glu Asn Trp Ser Gly Trp

245 250 255

ttc ctt ggt ttt gga gat cct tct cct tac aga cca gtt gaa gat ctt 816

Phe Leu Gly Phe Gly Asp Pro Ser Pro Tyr Arg Pro Val Glu Asp Leu

260 265 270

gca ttt gcg gtc gcg cgg ttt tac caa cga ggt gga acg ttc cag aac 864

Ala Phe Ala Val Ala Arg Phe Tyr Gln Arg Gly Gly Thr Phe Gln Asn

275 280 285

tat tac atg tat cac ggt gga aca aac ttt gat aga aca agt gga gga 912

Tyr Tyr Met Tyr His Gly Gly Thr Asn Phe Asp Arg Thr Ser Gly Gly

290 295 300

cca tta atc tct act agt tat gat tat gat gct cca att gat gag tat 960

Pro Leu Ile Ser Thr Ser Tyr Asp Tyr Asp Ala Pro Ile Asp Glu Tyr

305 310 315 320

gga cta ctt aga caa cca aaa tgg gga cac tta cga gat cta cac aag 1008

Gly Leu Leu Arg Gln Pro Lys Trp Gly His Leu Arg Asp Leu His Lys

325 330 335

gct atc aag ctt tgt gaa gat gca ttg att gcc aca gat cca aca att 1056

Ala Ile Lys Leu Cys Glu Asp Ala Leu Ile Ala Thr Asp Pro Thr Ile

340 345 350

act tct cta ggt tca aat ttg gag gct gct gta tat aaa aca gaa tct 1104

Thr Ser Leu Gly Ser Asn Leu Glu Ala Ala Val Tyr Lys Thr Glu Ser

355 360 365

gga tca tgt gct gct ttt ctt gca aat gtt gac acg aag tct gat gca 1152

Gly Ser Cys Ala Ala Phe Leu Ala Asn Val Asp Thr Lys Ser Asp Ala

370 375 380

act gtg act ttc aat gga aaa tca tat aac ttg cct gca tgg tcc gta 1200

Thr Val Thr Phe Asn Gly Lys Ser Tyr Asn Leu Pro Ala Trp Ser Val

385 390 395 400

agc atc ttg ccg gat tgc aaa aat gta gct ttc aat acc gca aag gta 1248

Ser Ile Leu Pro Asp Cys Lys Asn Val Ala Phe Asn Thr Ala Lys Val

405 410 415

aag ttc aat agc atc tct aaa act ccc gat ggt ggt tcg tct gcg gag 1296

Lys Phe Asn Ser Ile Ser Lys Thr Pro Asp Gly Gly Ser Ser Ala Glu

420 425 430

tta ggt tca caa tgg agt tac att aaa gaa cct att gga att tcc aaa 1344

Leu Gly Ser Gln Trp Ser Tyr Ile Lys Glu Pro Ile Gly Ile Ser Lys

435 440 445

gct gat gca ttc ttg aaa cct gga ttg cta gag cag att aac aca aca 1392

Ala Asp Ala Phe Leu Lys Pro Gly Leu Leu Glu Gln Ile Asn Thr Thr

450 455 460

gct gat aaa agc gat tac ttg tgg tac tca cta agg acg gat ata aaa 1440

Ala Asp Lys Ser Asp Tyr Leu Trp Tyr Ser Leu Arg Thr Asp Ile Lys

465 470 475 480

ggc gat gag act ttc ctt gac gag gga tct aaa gcc gtc ctt cac att 1488

Gly Asp Glu Thr Phe Leu Asp Glu Gly Ser Lys Ala Val Leu His Ile

485 490 495

gaa tct ctt ggt caa gtg gtc tat gct ttt ata aat gga aaa ctt gca 1536

Glu Ser Leu Gly Gln Val Val Tyr Ala Phe Ile Asn Gly Lys Leu Ala

500 505 510

gga agc gga cat ggc aaa cag aag att tct ttg gat ata ccg att aat 1584

Gly Ser Gly His Gly Lys Gln Lys Ile Ser Leu Asp Ile Pro Ile Asn

515 520 525

ctt gta acc ggg acg aac aca atc gat ctc ctt agt gtt acc gta ggg 1632

Leu Val Thr Gly Thr Asn Thr Ile Asp Leu Leu Ser Val Thr Val Gly

530 535 540

ctt gcg aat tat gga gct ttc ttt gac tta gtg gga gca gga ata acc 1680

Leu Ala Asn Tyr Gly Ala Phe Phe Asp Leu Val Gly Ala Gly Ile Thr

545 550 555 560

gga cct gtg aca ctt aaa agc gct aaa ggt ggt agc tca att gat ttg 1728

Gly Pro Val Thr Leu Lys Ser Ala Lys Gly Gly Ser Ser Ile Asp Leu

565 570 575

gct tca cag caa tgg act tat cag gtt gga ctc aaa gga gaa gac aca 1776

Ala Ser Gln Gln Trp Thr Tyr Gln Val Gly Leu Lys Gly Glu Asp Thr

580 585 590

ggt ttg gca act gta gat tct tct gaa tgg gtt tca aag tct cct ttg 1824

Gly Leu Ala Thr Val Asp Ser Ser Glu Trp Val Ser Lys Ser Pro Leu

595 600 605

cct act aaa caa cca ctt att tgg tac aag acg aca ttt gat gct cct 1872

Pro Thr Lys Gln Pro Leu Ile Trp Tyr Lys Thr Thr Phe Asp Ala Pro

610 615 620

tct ggg agc gag cca gta gct ata gac ttc acg ggt aca gga aag ggt 1920

Ser Gly Ser Glu Pro Val Ala Ile Asp Phe Thr Gly Thr Gly Lys Gly

625 630 635 640

att gca tgg gtg aat gga cag agc ata ggt agg tac tgg cca act agt 1968

Ile Ala Trp Val Asn Gly Gln Ser Ile Gly Arg Tyr Trp Pro Thr Ser

645 650 655

atc gct gga aat ggc ggt tgt aca gaa tca tgc gac tat aga ggt tct 2016

Ile Ala Gly Asn Gly Gly Cys Thr Glu Ser Cys Asp Tyr Arg Gly Ser

660 665 670

tac cgt gca aac aaa tgc ctc aag aac tgt gga aaa cct tca cag aca 2064

Tyr Arg Ala Asn Lys Cys Leu Lys Asn Cys Gly Lys Pro Ser Gln Thr

675 680 685

ttg tat cat gta cct cgc tcg tgg cta aaa ccg agc ggg aac ata ctt 2112

Leu Tyr His Val Pro Arg Ser Trp Leu Lys Pro Ser Gly Asn Ile Leu

690 695 700

gtt ctg ttt gag gag atg gga gga gat cca aca caa ata tca ttt gcg 2160

Val Leu Phe Glu Glu Met Gly Gly Asp Pro Thr Gln Ile Ser Phe Ala

705 710 715 720

aca aaa caa aca gga agc aat ctt tgt cta acg gtg tca cag tct cat 2208

Thr Lys Gln Thr Gly Ser Asn Leu Cys Leu Thr Val Ser Gln Ser His

725 730 735

cca cca ccg gtg gac aca tgg act tcc gac tca aag atc tca aac aga 2256

Pro Pro Pro Val Asp Thr Trp Thr Ser Asp Ser Lys Ile Ser Asn Arg

740 745 750

aac aga acc agg ccg gtt ctt tcg ttg aaa tgc cct atc tct act cag 2304

Asn Arg Thr Arg Pro Val Leu Ser Leu Lys Cys Pro Ile Ser Thr Gln

755 760 765

gtg ata ttt tct ata aaa ttt gca agc ttt ggt aca ccc aaa ggt act 2352

Val Ile Phe Ser Ile Lys Phe Ala Ser Phe Gly Thr Pro Lys Gly Thr

770 775 780

tgc ggt agc ttc aca caa ggc cat tgc aat agc tct cga tct ctc tcc 2400

Cys Gly Ser Phe Thr Gln Gly His Cys Asn Ser Ser Arg Ser Leu Ser

785 790 795 800

ctc gtc caa aag gca tgt att gga ttg agg agt tgc aac gtt gaa gta 2448

Leu Val Gln Lys Ala Cys Ile Gly Leu Arg Ser Cys Asn Val Glu Val

805 810 815

tcg act aga gtg ttc ggg gaa cct tgt cgt ggc gtc gtc aag agc tta 2496

Ser Thr Arg Val Phe Gly Glu Pro Cys Arg Gly Val Val Lys Ser Leu

820 825 830

gct gtt gaa gct tct tgt tca tga 2520

Ala Val Glu Ala Ser Cys Ser

835

<210>102

<211>839

<212>PRT

＜213〉Arabidopis thaliana

<400>102

Met Val Lys Val Arg Lys Met Glu Met Ile Leu Leu Leu Ile Leu Val

1 5 10 15

Ile Val Val Ala Ala Thr Ala Ala Asn Val Thr Tyr Asp His Arg Ala

20 25 30

Leu Val Ile Asp Gly Lys Arg Lys Val Leu Ile Ser Gly Ser Ile His

35 40 45

Tyr Pro Arg Ser Thr Pro Glu Met Trp Pro Glu Leu Ile Gln Lys Ser

50 55 60

Lys Asp Gly Gly Leu Asp Val Ile Glu Thr Tyr Val Phe Trp Ser Gly

65 70 75 80

His Glu Pro Glu Lys Asn Lys Tyr Asn Phe Glu Gly Arg Tyr Asp Leu

85 90 95

Val Lys Phe Val Lys Leu Ala Ala Lys Ala Gly Leu Tyr Val His Leu

100 105 110

Arg Ile Gly Pro Tyr Val Cys Ala Glu Trp Asn Tyr Gly Gly Phe Pro

115 120 125

Val Trp Leu His Phe Val Pro Gly Ile Lys Phe Arg Thr Asp Asn Glu

130 135 140

Pro Phe Lys Glu Glu Met Gln Arg Phe Thr Thr Lys Ile Val Asp Leu

145 150 155 160

Met Lys Gln Glu Lys Leu Tyr Ala Ser Gln Gly Gly Pro Ile Ile Leu

165 170 175

Ser Gln Ile Glu Asn Glu Tyr Gly Asn Ile Asp Ser Ala Tyr Gly Ala

180 185 190

Ala Ala Lys Ser Tyr Ile Lys Trp Ser Ala Ser Met Ala Leu Ser Leu

195 200 205

Asp Thr Gly Val Pro Trp Asn Met Cys Gln Gln Thr Asp Ala Pro Asp

210 215 220

Pro Met Ile Asn Thr Cys Asn Gly Phe Tyr Cys Asp Gln Phe Thr Pro

225 230 235 240

Asn Ser Asn Asn Lys Pro Lys Met Trp Thr Glu Asn Trp Ser Gly Trp

245 250 255

Phe Leu Gly Phe Gly Asp Pro Ser Pro Tyr Arg Pro Val Glu Asp Leu

260 265 270

Ala Phe Ala Val Ala Arg Phe Tyr Gln Arg Gly Gly Thr Phe Gln Asn

275 280 285

Tyr Tyr Met Tyr His Gly Gly Thr Asn Phe Asp Arg Thr Ser Gly Gly

290 295 300

Pro Leu Ile Ser Thr Ser Tyr Asp Tyr Asp Ala Pro Ile Asp Glu Tyr

305 310 315 320

Gly Leu Leu Arg Gln Pro Lys Trp Gly His Leu Arg Asp Leu His Lys

325 330 335

Ala Ile Lys Leu Cys Glu Asp Ala Leu Ile Ala Thr Asp Pro Thr Ile

340 345 350

Thr Ser Leu Gly Ser Asn Leu Glu Ala Ala Val Tyr Lys Thr Glu Ser

355 360 365

Gly Ser Cys Ala Ala Phe Leu Ala Asn Val Asp Thr Lys Ser Asp Ala

370 375 380

Thr Val Thr Phe Asn Gly Lys Ser Tyr Asn Leu Pro Ala Trp Ser Val

385 390 395 400

Ser Ile Leu Pro Asp Cys Lys Asn Val Ala Phe Asn Thr Ala Lys Val

405 410 415

Lys Phe Asn Ser Ile Ser Lys Thr Pro Asp Gly Gly Ser Ser Ala Glu

420 425 430

Leu Gly Ser Gln Trp Ser Tyr Ile Lys Glu Pro Ile Gly Ile Ser Lys

435 440 445

Ala Asp Ala Phe Leu Lys Pro Gly Leu Leu Glu Gln Ile Asn Thr Thr

450 455 460

Ala Asp Lys Ser Asp Tyr Leu Trp Tyr Ser Leu Arg Thr Asp Ile Lys

465 470 475 480

Gly Asp Glu Thr Phe Leu Asp Glu Gly Ser Lys Ala Val Leu His Ile

485 490 495

Glu Ser Leu Gly Gln Val Val Tyr Ala Phe Ile Asn Gly Lys Leu Ala

500 505 510

Gly Ser Gly His Gly Lys Gln Lys Ile Ser Leu Asp Ile Pro Ile Asn

515 520 525

Leu Val Thr Gly Thr Asn Thr Ile Asp Leu Leu Ser Val Thr Val Gly

530 535 540

Leu Ala Asn Tyr Gly Ala Phe Phe Asp Leu Val Gly Ala Gly Ile Thr

545 550 555 560

Gly Pro Val Thr Leu Lys Ser Ala Lys Gly Gly Ser Ser Ile Asp Leu

565 570 575

Ala Ser Gln Gln Trp Thr Tyr Gln Val Gly Leu Lys Gly Glu Asp Thr

580 585 590

Gly Leu Ala Thr Val Asp Ser Ser Glu Trp Val Ser Lys Ser Pro Leu

595 600 605

Pro Thr Lys Gln Pro Leu Ile Trp Tyr Lys Thr Thr Phe Asp Ala Pro

610 615 620

Ser Gly Ser Glu Pro Val Ala Ile Asp Phe Thr Gly Thr Gly Lys Gly

625 630 635 640

Ile Ala Trp Val Asn Gly Gln Ser Ile Gly Arg Tyr Trp Pro Thr Ser

645 650 655

Ile Ala Gly Asn Gly Gly Cys Thr Glu Ser Cys Asp Tyr Arg Gly Ser

660 665 670

Tyr Arg Ala Asn Lys Cys Leu Lys Asn Cys Gly Lys Pro Ser Gln Thr

675 680 685

Leu Tyr His Val Pro Arg Ser Trp Leu Lys Pro Ser Gly Asn Ile Leu

690 695 700

Val Leu Phe Glu Glu Met Gly Gly Asp Pro Thr Gln Ile Ser Phe Ala

705 710 715 720

Thr Lys Gln Thr Gly Ser Asn Leu Cys Leu Thr Val Ser Gln Ser His

725 730 735

Pro Pro Pro Val Asp Thr Trp Thr Ser Asp Ser Lys Ile Ser Asn Arg

740 745 750

Asn Arg Thr Arg Pro Val Leu Ser Leu Lys Cys Pro Ile Ser Thr Gln

755 760 765

Val Ile Phe Ser Ile Lys Phe Ala Ser Phe Gly Thr Pro Lys Gly Thr

770 775 780

Cys Gly Ser Phe Thr Gln Gly His Cys Asn Ser Ser Arg Ser Leu Ser

785 790 795 800

Leu Val Gln Lys Ala Cys Ile Gly Leu Arg Ser Cys Asn Val Glu Val

805 810 815

Ser Thr Arg Val Phe Gly Glu Pro Cys Arg Gly Val Val Lys Ser Leu

820 825 830

Ala Val Glu Ala Ser Cys Ser

835

<210>103

<211>771

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(771)

<400>103

atg gtt gga ccc gcg cgg cct cag atc gtt ttg ttt gga tct tcc att 48

Met Val Gly Pro Ala Arg Pro Gln Ile Val Leu Phe Gly Ser Ser Ile

1 5 10 15

gtt cag atg agc ttt ggc cat ggt ggt tgg ggc gcc att ctt tcc gag 96

Val Gln Met Ser Phe Gly His Gly Gly Trp Gly Ala Ile Leu Ser Glu

20 25 30

gtc tac gct cgt aag gcc gac atc att ctg cga gga tat tat gga tgg 144

Val Tyr Ala Arg Lys Ala Asp Ile Ile Leu Arg Gly Tyr Tyr Gly Trp

35 40 45

aac tct tct cgt gct ttg gaa gtt gtc gac caa gtg ttc ccc aag gat 192

Asn Ser Ser Arg Ala Leu Glu Val Val Asp Gln Val Phe Pro Lys Asp

50 55 60

gct gca gta caa cct tct ctg gtc att gtc tat ttt gga gga aac gac 240

Ala Ala Val Gln Pro Ser Leu Val Ile Val Tyr Phe Gly Gly Asn Asp

65 70 75 80

tca atg gcg cct cac tcg tct gga cta gga cct cat gta cca ctt act 288

Ser Met Ala Pro His Ser Ser Gly Leu Gly Pro His Val Pro Leu Thr

85 90 95

gaa tat gtt gat aac atg aag aag atc gct ctt cat ctt cag agc ctt 336

Glu Tyr Val Asp Asn Met Lys Lys Ile Ala Leu His Leu Gln Ser Leu

100 105 110

tca gac ttc acc cga atc ata ttt ctt agt tct cct cca gtg gat gag 384

Ser Asp Phe Thr Arg Ile Ile Phe Leu Ser Ser Pro Pro Val Asp Glu

115 120 125

gct aaa gtt cgc cag aac caa agc cca tac ttg agc gag gta atc cgc 432

Ala Lys Val Arg Gln Asn Gln Ser Pro Tyr Leu Ser Glu ValIle Arg

130 135 140

aca aac gac ctc tgc aag act tat tca gat gct tgt gta gag ctg tgc 480

Thr Asn Asp Leu Cys Lys Thr Tyr Ser Asp Ala Cys Val Glu Leu Cys

145 150 155 160

caa gaa ctc ggc cta gaa gta gtt gat ctc ttc tct act ttt cag aaa 528

Gln Glu Leu Gly Leu Glu Val Val Asp Leu Phe Ser Thr Phe Gln Lys

165 170 175

gca gat gac tgg aaa act gtt tgc ttc aca gac ggg att cat ttg tca 576

Ala Asp Asp Trp Lys Thr Val Cys Phe Thr Asp Gly Ile His Leu Ser

180 185 190

gca caa gga agc aaa ata gta gcg gga gag ata cta aga gtg gtt aaa 624

Ala Gln Gly Ser Lys Ile Val Ala Gly Glu Ile Leu Arg Val Val Lys

195 200 205

gaa gcg gaa tgg cat cca tca ctt cac tgg aaa tca atg cca aca gaa 672

Glu Ala Glu Trp His Pro Ser Leu His Trp Lys Ser Met Pro Thr Glu

210 215 220

ttc gca gat gac tct cct tat gat ctt gta tca gca gat ggc aaa cag 720

Phe Ala Asp Asp Ser Pro Tyr Asp Leu Val Ser Ala Asp Gly Lys Gln

225 230 235 240

aca gta aat tct tca gaa tgg act tat ttc tgg gaa gaa caa tgg gac 768

Thr Val Asn Ser Ser Glu Trp Thr Tyr Phe Trp Glu Glu Gln Trp Asp

245 250 255

taa 771

<210>104

<211>256

<212>PRT

＜213〉Arabidopis thaliana

<400>104

Met Val Gly Pro Ala Arg Pro Gln Ile Val Leu Phe Gly Ser Ser Ile

1 5 10 15

Val Gln Met Ser Phe Gly His Gly Gly Trp Gly Ala Ile Leu Ser Glu

20 25 30

Val Tyr Ala Arg Lys Ala Asp Ile Ile Leu Arg Gly Tyr Tyr Gly Trp

35 40 45

Asn Ser Ser Arg Ala Leu Glu Val Val Asp Gln Val Phe Pro Lys Asp

50 55 60

Ala Ala Val Gln Pro Ser Leu Val Ile Val Tyr Phe Gly Gly Asn Asp

65 70 75 80

Ser Met Ala Pro His Ser Ser Gly Leu Gly Pro His Val Pro Leu Thr

85 90 95

Glu Tyr Val Asp Asn Met Lys Lys Ile Ala Leu His Leu Gln Ser Leu

100 105 110

Ser Asp Phe Thr Arg Ile Ile Phe Leu Ser Ser Pro Pro Val Asp Glu

115 120 125

Ala Lys Val Arg Gln Asn Gln Ser Pro Tyr Leu Ser Glu Val Ile Arg

130 135 140

Thr Asn Asp Leu Cys Lys Thr Tyr Ser Asp Ala Cys Val Glu Leu Cys

145 150 155 160

Gln Glu Leu Gly Leu Glu Val Val Asp Leu Phe Ser Thr Phe Gln Lys

165 170 175

Ala Asp Asp Trp Lys Thr Val Cys Phe Thr Asp Gly Ile His Leu Ser

180 185 190

Ala Gln Gly Ser Lys Ile Val Ala Gly Glu Ile Leu Arg Val Val Lys

195 200 205

Glu Ala Glu Trp His Pro Ser Leu His Trp Lys Ser Met Pro Thr Glu

210 215 220

Phe Ala Asp Asp Ser Pro Tyr Asp Leu Val Ser Ala Asp Gly Lys Gln

225 230 235 240

Thr Val Asn Ser Ser Glu Trp Thr Tyr Phe Trp Glu Glu Gln Trp Asp

245 250 255

<210>105

<211>2466

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(2466)

<400>105

atg gaa atg ccc ggt aga aga tct aat tac act ttg ctt agt caa ttt 48

Met Glu Met Pro Gly Arg Arg Ser Asn Tyr Thr Leu Leu Ser Gln Phe

1 5 10 15

tct gac gat cag gtg tca gtt tcc gtc acc gga gct cct ccg cct cac 96

Ser Asp Asp Gln Val Ser Val Ser Val Thr Gly Ala Pro Pro Pro His

20 25 30

tat gat tcc ttg tcg agc gaa aac agg agc aac cat aac agc ggg aac 144

Tyr Asp Ser Leu Ser Ser Glu Asn Arg Ser Asn His Asn Ser Gly Asn

35 40 45

acc ggg aaa gct aag gcg gag aga ggc gga ttt gat tgg gat cct agc 192

Thr Gly Lys Ala Lys Ala Glu Arg Gly Gly Phe Asp Trp Asp Pro Ser

50 55 60

ggt ggt ggt ggt ggt gat cat agg ttg aat aat caa ccg aat cgg gtt 240

Gly Gly Gly Gly Gly Asp His Arg Leu Asn Asn Gln Pro Asn Arg Val

65 70 75 80

ggg aat aat atg tat gct tcg tct cta ggg ttg caa agg caa tcc agt 288

Gly Asn Asn Met Tyr Ala Ser Ser Leu Gly Leu Gln Arg Gln Ser Ser

85 90 95

ggg agt agt ttc ggt gag agc tct ttg tct ggg gat tat tac atg cct 336

Gly Ser Ser Phe Gly Glu Ser Ser Leu Ser Gly Asp Tyr Tyr Met Pro

100 105 110

acg ctt tct gcg gcg gct aac gag atc gaa tct gtt gga ttt cct caa 384

Thr Leu Ser Ala Ala Ala Asn Glu Ile Glu Ser Val Gly Phe Pro Gln

115 120 125

gat gat ggg ttt agg ctt gga ttt ggt ggt ggt gga gga gat ttg agg 432

Asp Asp Gly Phe Arg Leu Gly Phe Gly Gly Gly Gly Gly Asp Leu Arg

130 135 140

ata cag atg gcg gcg gac tcc gct gga ggg tct tca tct ggg aag agc 480

Ile Gln Met Ala Ala Asp Ser Ala Gly Gly Ser Ser Ser Gly Lys Ser

145 150 155 160

tgg gcg cag cag acg gag gag agt tat cag ctg cag ctt gca ttg gcg 528

Trp Ala Gln Gln Thr Glu Glu Ser Tyr Gln Leu Gln Leu Ala Leu Ala

165 170 175

tta agg ctt tcg tcg gag gct act tgt gcc gac gat ccg aac ttt ctg 576

Leu Arg Leu Ser Ser Glu Ala Thr Cys Ala Asp Asp Pro Asn Phe Leu

180 185 190

gat cct gta ccg gac gag tct gct tta cgg act tcg cca agt tca gcc 624

Asp Pro Val Pro Asp Glu Ser Ala Leu Arg Thr Ser Pro Ser Ser Ala

195 200 205

gaa acc gtt tca cat cgt ttc tgg gtt aat ggc tgc tta tcg tac tat 672

Glu Thr Val Ser His Arg Phe Trp Val Asn Gly Cys Leu Ser Tyr Tyr

210 215 220

gat aaa gtt cct gat ggg ttt tat atg atg aat ggt ctg gat ccc tat 720

Asp Lys Val Pro Asp Gly Phe Tyr Met Met Asn Gly Leu Asp Pro Tyr

225 230 235 240

att tgg acc tta tgc atc gac ctg cat gaa agt ggt cgc atc cct tca 768

Ile Trp Thr Leu Cys Ile Asp Leu His Glu Ser Gly Arg Ile Pro Ser

245 250 255

att gaa tca tta aga gct gtt gat tct ggt gtt gat tct tcg ctt gaa 816

Ile Glu Ser Leu Arg Ala Val Asp Ser Gly Val Asp Ser Ser Leu Glu

260 265 270

gcg atc ata gtt gat agg cgt agt gat cca gcc ttc aag gaa ctt cac 864

Ala Ile Ile Val Asp Arg Arg Ser Asp Pro Ala Phe Lys Glu Leu His

275 280 285

aat aga gtc cac gac ata tct tgt agc tgc att acc aca aaa gag gtt 912

Asn Arg Val His Asp Ile Ser Cys Ser Cys Ile Thr Thr Lys Glu Val

290 295 300

gtt gat cag ctg gca aag ctt atc tgc aat cgt atg ggg ggt cca gtt 960

Val Asp Gln Leu Ala Lys Leu Ile Cys Asn Arg Met Gly Gly Pro Val

305 310 315 320

atc atg ggg gaa gat gag ttg gtt ccc atg tgg aag gag tgc att gat 1008

Ile Met Gly Glu Asp Glu Leu Val Pro Met Trp Lys Glu Cys Ile Asp

325 330 335

ggt cta aaa gaa atc ttt aaa gtg gtg gtt ccc ata ggt agc ctc tct 1056

Gly Leu Lys Glu Ile Phe Lys Val Val Val Pro Ile Gly Ser Leu Ser

340 345 350

gtt gga ctc tgc aga cat cga gct tta ctc ttc aaa gta ctg gct gac 1104

Val Gly Leu Cys Arg His Arg Ala Leu Leu Phe Lys Val Leu Ala Asp

355 360 365

ata att gat tta ccc tgt cga att gcc aaa gga tgt aaa tat tgt aat 1152

Ile Ile Asp Leu Pro Cys Arg Ile Ala Lys Gly Cys Lys Tyr Cys Asn

370 375 380

aga gac gat gcc gct tcg tgc ctt gtc agg ttt ggg ctt gat agg gag 1200

Arg Asp Asp Ala Ala Ser Cys Leu Val Arg Phe Gly Leu Asp Arg Glu

385 390 395 400

tac ctg gtt gat tta gta gga aag cca ggt cac tta tgg gag cct gat 1248

Tyr Leu Val Asp Leu Val Gly Lys Pro Gly His Leu Trp Glu Pro Asp

405 410 415

tcc ttg cta aat ggt cct tca tct atc tca att tct tct cct ctg cgg 1296

Ser Leu Leu Asn Gly Pro Ser Ser Ile Ser Ile Ser Ser Pro Leu Arg

420 425 430

ttt cca cga cca aag cca gtt gaa ccc gca gtc gat ttt agg tta cta 1344

Phe Pro Arg Pro Lys Pro Val Glu Pro Ala Val Asp Phe Arg Leu Leu

435 440 445

gcc aaa caa tat ttc tcc gat agc cag tct ctt aat ctt gtt ttc gat 1392

Ala Lys Gln Tyr Phe Ser Asp Ser Gln Ser Leu Asn Leu Val Phe Asp

450 455 460

cct gca tca gat gat atg gga ttc tca atg ttt cat agg caa tat gat 1440

Pro Ala Ser Asp Asp Met Gly Phe Ser Met Phe His Arg Gln Tyr Asp

465 470 475 480

aat ccg ggt gga gag aat gac gca ttg gca gaa aat ggt ggt ggg tct 1488

Asn Pro Gly Gly Glu Asn Asp Ala Leu Ala Glu Asn Gly Gly Gly Ser

485 490 495

ttg cca ccc agt gct aat atg cct cca cag aac atg atg cgt gcg tca 1536

Leu Pro Pro Ser Ala Asn Met Pro Pro Gln Asn Met Met Arg Ala Ser

500 505 510

aat caa att gaa gca gca cct atg aat gcc cca cca atc agt cag cca 1584

Asn Gln Ile Glu Ala Ala Pro Met Asn Ala Pro Pro Ile Ser Gln Pro

515 520 525

gtt cca aac agg gca aat agg gaa ctt gga ctt gat ggt gat gat atg 1632

Val Pro Asn Arg Ala Asn Arg Glu Leu Gly Leu Asp Gly Asp Asp Met

530 535 540

gac atc ccg tgg tgt gat ctt aat ata aaa gaa aag att gga gca ggt 1680

Asp Ile Pro Trp Cys Asp Leu Asn Ile Lys Glu Lys Ile Gly Ala Gly

545 550 555 560

tcc ttt ggc act gtc cac cgt gct gag tgg cat ggc tcg gat gtt gct 1728

Ser Phe Gly Thr Val His Arg Ala Glu Trp His Gly Ser Asp Val Ala

565 570 575

gtg aaa att ctc atg gag caa gac ttc cat gct gag cgt gtt aat gag 1776

Val Lys Ile Leu Met Glu Gln Asp Phe His Ala Glu Arg Val Asn Glu

580 585 590

ttc tta aga gag gtt gcg ata atg aaa cgc ctt cgc cac cct aac att 1824

Phe Leu Arg Glu Val Ala Ile Met Lys Arg Leu Arg His Pro Asn Ile

595 600 605

gtt ctc ttc atg ggt gcg gtc act caa cct cca aat ttg tca ata gtg 1872

Val Leu Phe Met Gly Ala Val Thr Gln Pro Pro Asn Leu Ser Ile Val

610 615 620

aca gaa tat ttg tca aga ggt agt tta tac aga ctt ttg cat aaa agt 1920

Thr Glu Tyr Leu Ser Arg Gly Ser Leu Tyr Arg Leu Leu His Lys Ser

625 630 635 640

gga gca agg gag caa tta gat gag aga cgt cgc ctg agt atg gct tat 1968

Gly Ala Arg Glu Gln Leu Asp Glu Arg Arg Arg Leu Ser Met Ala Tyr

645 650 655

gat gtg gct aag gga atg aat tat ctt cac aat cgc aat cct cca att 2016

Asp Val Ala Lys Gly Met Asn Tyr Leu His Asn Arg Asn Pro Pro Ile

660 665 670

gtg cat aga gat cta aaa tct cca aac tta ttg gtt gac aaa aaa tat 2064

Val His Arg Asp Leu Lys Ser Pro Asn Leu Leu Val Asp Lys Lys Tyr

675 680 685

aca gtc aag gtt tgt gat ttt ggt ctc tcg cga ttg aag gcc agc acg 2112

Thr Val Lys Val Cys Asp Phe Gly Leu Ser Arg Leu Lys Ala Ser Thr

690 695 700

ttt ctt tcc tcg aag tca gca gct gga acc ccc gag tgg atg gca cca 2160

Phe Leu Ser Ser Lys Ser Ala Ala Gly Thr Pro Glu Trp Met Ala Pro

705 710 715 720

gaa gtc ctg cga gat gag ccg tct aat gaa aag tca gat gtg tac agc 2208

Glu Val Leu Arg Asp Glu Pro Ser Asn Glu Lys Ser Asp Val Tyr Ser

725 730 735

ttc ggg gtc atc ttg tgg gag ctt gct aca ttg caa caa cca tgg ggt 2256

Phe Gly Val Ile Leu Trp Glu Leu Ala Thr Leu Gln Gln Pro Trp Gly

740 745 750

aac tta aat ccg gct cag gtt gta gct gcg gtt ggt ttc aag tgt aaa 2304

Asn Leu Asn Pro Ala Gln Val Val Ala Ala Val Gly Phe Lys Cys Lys

755 760 765

cgg ctg gag atc ccg cgt aat ctg aat cct cag gtt gca gcc ata atc 2352

Arg Leu Glu Ile Pro Arg Asn Leu Asn Pro Gln Val Ala Ala Ile Ile

770 775 780

gag ggt tgt tgg acc aat gag cca tgg aag cgt cca tca ttt gca act 2400

Glu Gly Cys Trp Thr Asn Glu Pro Trp Lys Arg Pro Ser Phe Ala Thr

785 790 795 800

ata atg gac ttg cta aga cca ttg atc aaa tca gcg gtt cct ccg ccc 2448

Ile Met Asp Leu Leu Arg Pro Leu Ile Lys Ser Ala Val Pro Pro Pro

805 810 815

aac cgc tcg gat ttg taa 2466

Asn Arg Ser Asp Leu

820

<210>106

<211>821

<212>PRT

＜213〉Arabidopis thaliana

<400>106

Met Glu Met Pro Gly Arg Arg Ser Asn Tyr Thr Leu Leu Ser Gln Phe

1 5 10 15

Ser Asp Asp Gln Val Ser Val Ser Val Thr Gly Ala Pro Pro Pro His

20 25 30

Tyr Asp Ser Leu Ser Ser Glu Asn Arg Ser Asn His Asn Ser Gly Asn

35 40 45

Thr Gly Lys Ala Lys Ala Glu Arg Gly Gly Phe Asp Trp Asp Pro Ser

50 55 60

Gly Gly Gly Gly Gly Asp His Arg Leu Asn Asn Gln Pro Asn Arg Val

65 70 75 80

Gly Asn Asn Met Tyr Ala Ser Ser Leu Gly Leu Gln Arg Gln Ser Ser

85 90 95

Gly Ser Ser Phe Gly Glu Ser Ser Leu Ser Gly Asp Tyr Tyr Met Pro

100 105 110

Thr Leu Ser Ala Ala Ala Asn Glu Ile Glu Ser Val Gly Phe Pro Gln

115 120 125

Asp Asp Gly Phe Arg Leu Gly Phe Gly Gly Gly Gly Gly Asp Leu Arg

130 135 140

Ile Gln Met Ala Ala Asp Ser Ala Gly Gly Ser Ser Ser Gly Lys Ser

145 150 155 160

Trp Ala Gln Gln Thr Glu Glu Ser Tyr Gln Leu Gln Leu Ala Leu Ala

165 170 175

Leu Arg Leu Ser Ser Glu Ala Thr Cys Ala Asp Asp Pro Asn Phe Leu

180 185 190

Asp Pro Val Pro Asp Glu Ser Ala Leu Arg Thr Ser Pro Ser Ser Ala

195 200 205

Glu Thr Val Ser His Arg Phe Trp Val Asn Gly Cys Leu Ser Tyr Tyr

210 215 220

Asp Lys Val Pro Asp Gly Phe Tyr Met Met Asn Gly Leu Asp Pro Tyr

225 230 235 240

Ile Trp Thr Leu Cys Ile Asp Leu His Glu Ser Gly Arg Ile Pro Ser

245 250 255

Ile Glu Ser Leu Arg Ala Val Asp Ser Gly Val Asp Ser Ser Leu Glu

260 265 270

Ala Ile Ile Val Asp Arg Arg Ser Asp Pro Ala Phe Lys Glu Leu His

275 280 285

Asn Arg Val His Asp Ile Ser Cys Ser Cys Ile Thr Thr Lys Glu Val

290 295 300

Val Asp Gln Leu Ala Lys Leu Ile Cys Asn Arg Met Gly Gly Pro Val

305 310 315 320

Ile Met Gly Glu Asp Glu Leu Val Pro Met Trp Lys Glu Cys Ile Asp

325 330 335

Gly Leu Lys Glu Ile Phe Lys Val Val Val Pro Ile Gly Ser Leu Ser

340 345 350

Val Gly Leu Cys Arg His Arg Ala Leu Leu Phe Lys Val Leu Ala Asp

355 360 365

Ile Ile Asp Leu Pro Cys Arg Ile Ala Lys Gly Cys Lys Tyr Cys Asn

370 375 380

Arg Asp Asp Ala Ala Ser Cys Leu Val Arg Phe Gly Leu Asp Arg Glu

385 390 395 400

Tyr Leu Val Asp Leu Val Gly Lys Pro Gly His Leu Trp Glu Pro Asp

405 410 415

Ser Leu Leu Asn Gly Pro Ser Ser Ile Ser Ile Ser Ser Pro Leu Arg

420 425 430

Phe Pro Arg Pro Lys Pro Val Glu Pro Ala Val Asp Phe Arg Leu Leu

435 440 445

Ala Lys Gln Tyr Phe Ser Asp Ser Gln Ser Leu Asn Leu Val Phe Asp

450 455 460

Pro Ala Ser Asp Asp Met Gly Phe Ser Met Phe His Arg Gln Tyr Asp

465 470 475 480

Asn Pro Gly Gly Glu Asn Asp Ala Leu Ala Glu Asn Gly Gly Gly Ser

485 490 495

Leu Pro Pro Ser Ala Asn Met Pro Pro Gln Asn Met Met Arg Ala Ser

500 505 510

Asn Gln Ile Glu Ala Ala Pro Met Asn Ala Pro Pro Ile Ser Gln Pro

515 520 525

Val Pro Asn Arg Ala Asn Arg Glu Leu Gly Leu Asp Gly Asp Asp Met

530 535 540

Asp Ile Pro Trp Cys Asp Leu Asn Ile Lys Glu Lys Ile Gly Ala Gly

545 550 555 560

Ser Phe Gly Thr Val His Arg Ala Glu Trp His Gly Ser Asp Val Ala

565 570 575

Val Lys Ile Leu Met Glu Gln Asp Phe His Ala Glu Arg Val Asn Glu

580 585 590

Phe Leu Arg Glu Val Ala Ile Met Lys Arg Leu Arg His Pro Asn Ile

595 600 605

Val Leu Phe Met Gly Ala Val Thr Gln Pro Pro Asn Leu Ser Ile Val

610 615 620

Thr Glu Tyr Leu Ser Arg Gly Ser Leu Tyr Arg Leu Leu His Lys Ser

625 630 635 640

Gly Ala Arg Glu Gln Leu Asp Glu Arg Arg Arg Leu Ser Met Ala Tyr

645 650 655

Asp Val Ala Lys Gly Met Asn Tyr Leu His Asn Arg Asn Pro Pro Ile

660 665 670

Val His Arg Asp Leu Lys Ser Pro Asn Leu Leu Val Asp Lys Lys Tyr

675 680 685

Thr Val Lys Val Cys Asp Phe Gly Leu Ser Arg Leu Lys Ala Ser Thr

690 695 700

Phe Leu Ser Ser Lys Ser Ala A1a Gly Thr Pro Glu Trp Met Ala Pro

705 710 715 720

Glu Val Leu Arg Asp Glu Pro Ser Asn Glu Lys Ser Asp Val Tyr Ser

725 730 735

Phe Gly Val Ile Leu Trp Glu Leu Ala Thr Leu Gln Gln Pro Trp Gly

740 745 750

Asn Leu Asn Pro Ala Gln Val Val Ala Ala Val Gly Phe Lys Cys Lys

755 760 765

Arg Leu Glu Ile Pro Arg Asn Leu Asn Pro Gln Val Ala Ala Ile Ile

770 775 780

Glu Gly Cys Trp Thr Asn Glu Pro Trp Lys Arg Pro Ser Phe Ala Thr

785 790 795 800

Ile Met Asp Leu Leu Arg Pro Leu Ile Lys Ser Ala Val Pro Pro Pro

805 810 815

Asn Arg Ser Asp Leu

820

<210>107

<211>1239

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(1239)

<400>107

atg gct aca aca acc aca tca tgg gaa gaa ctc tta ggc tca aag aat 48

Met Ala Thr Thr Thr Thr Ser Trp Glu Glu Leu Leu Gly Ser Lys Asn

1 5 10 15

tgg gac act atc tta gac cca tta gac caa tca ctt agg gaa ctc atc 96

Trp Asp Thr Ile Leu Asp Pro Leu Asp Gln Ser Leu Arg Glu Leu Ile

20 25 30

tta cgt tgt ggc gac ttt tgt caa gcc acc tac gat gcc ttc gtc aac 144

Leu Arg Cys Gly Asp Phe Cys Gln Ala Thr Tyr Asp Ala Phe Val Asn

35 40 45

gac caa aac tcc aag tac tgt gga gcc agc cgc tac ggc aaa tct tct 192

Asp Gln Asn Ser Lys Tyr Cys Gly Ala Ser Arg Tyr Gly Lys Ser Ser

50 55 60

ttc ttc gac aag gtc atg ctc gaa aac gct tcc gac tac gag gtt gta 240

Phe Phe Asp Lys Val Met Leu Glu Asn Ala Ser Asp Tyr Glu Val Val

65 70 75 80

aac ttc ctc tac gcc aca gct cgt gtt tct ctc ccc gaa ggt ttg ctt 288

Asn Phe Leu Tyr Ala Thr Ala Arg Val Ser Leu Pro Glu Gly Leu Leu

85 90 95

ctc caa tca caa tca aga gat tct tgg gac cgt gag tct aac tgg ttt 336

Leu Gln Ser Gln Ser Arg Asp Ser Trp Asp Arg Glu Ser Asn Trp Phe

100 105 110

ggc tac att gct gtc acg tct gat gaa cgg tct aag gct tta gga cgc 384

Gly Tyr Ile Ala Val Thr Ser Asp Glu Arg Ser Lys Ala Leu Gly Arg

115 120 125

cgt gag atc tat ata gct ttg aga gga acg agc agg aac tat gag tgg 432

Arg Glu Ile Tyr Ile Ala Leu Arg Gly Thr Ser Arg Asn Tyr Glu Trp

130 135 140

gtc aat gtt ttg ggt gct agg cca act tca gct gac ccc ttg ctg cac 480

Val Asn Val Leu Gly Ala Arg Pro Thr Ser Ala Asp Pro Leu Leu His

145 150 155 160

gga ccc gag cag gat ggt tct ggt ggt gta gtt gaa ggt acg act ttt 528

Gly Pro Glu Gln Asp Gly Ser Gly Gly Val Val Glu Gly Thr Thr Phe

165 170 175

gat agt gac agt gaa gat gaa gaa ggg tgt aag gtg atg ctc ggg tgg 576

Asp Ser Asp Ser Glu Asp Glu Glu Gly Cys Lys Val Met Leu Gly Trp

180 185 190

ctc aca atc tat act tct aat cac ccc gaa tcg aaa ttc act aag ctg 624

Leu Thr Ile Tyr Thr Ser Asn His Pro Glu Ser Lys Phe Thr Lys Leu

195 200 205

agt cta cgg tca cag ttg tta gcc aag atc aag gag ctt ctg ttg aag 672

Ser Leu Arg Ser Gln Leu Leu Ala Lys Ile Lys Glu Leu Leu Leu Lys

210 215 220

tat aag gac gag aaa ccg agc att gtg ttg act gga cat agc ttg gga 720

Tyr Lys Asp Glu Lys Pro Ser Ile Val Leu Thr Gly His Ser Leu Gly

225 230 235 240

gct aca gag gct gtt ctg gcc gcc tat gat ata gct gag aac ggt tcc 768

Ala Thr Glu Ala Val Leu Ala Ala Tyr Asp Ile Ala Glu Asn Gly Ser

245 250 255

agt gat gat gtt ccg gtc act gct ata gtc ttt ggt tgt cca cag gta 816

Ser Asp Asp Val Pro Val Thr Ala Ile Val Phe Gly Cys Pro Gln Val

260 265 270

gga aac aag gag ttc aga gac gaa gta atg agt cac aag aac tta aag 864

Gly Asn Lys Glu Phe Arg Asp Glu Val Met Ser His Lys Asn Leu Lys

275 280 285

atc ctc cat gta agg aac acg att gat ctc tta act cga tac cca ggg 912

Ile Leu His Val Arg Asn Thr Ile Asp Leu Leu Thr Arg Tyr Pro Gly

290 295 300

gga ctt tta ggg tat gtg gac ata gga ata aac ttt gtg atc gat aca 960

Gly Leu Leu Gly Tyr Val Asp Ile Gly Ile Asn Phe Val Ile Asp Thr

305 310 315 320

aag aag tca ccg ttc cta agc gat tca agg aat cca ggg gat tgg cat 1008

Lys Lys Ser Pro Phe Leu Ser Asp Ser Arg Asn Pro Gly Asp Trp His

325 330 335

aat ctt cag gcg atg tta cat gtt gta gct gga tgg aat ggg aag aaa 1056

Asn Leu Gln Ala Met Leu His Val Val Ala Gly Trp Asn Gly Lys Lys

340 345 350

gga gag ttt aaa ctg atg gtt aag aga agt att gca tta gtg aac aag 1104

Gly Glu Phe Lys Leu Met Val Lys Arg Ser Ile Ala Leu Val Asn Lys

355 360 365

tca tgc gag ttc ttg aaa gct gag tgt ttg gtg cca gga tct tgg tgg 1152

Ser Cys Glu Phe Leu Lys Ala Glu Cys Leu Val Pro Gly Ser Trp Trp

370 375 380

gta gag aag aac aaa gga ctg atc aag aac gaa gat ggt gaa tgg gtt 1200

Val Glu Lys Asn Lys Gly Leu Ile Lys Asn Glu Asp Gly Glu Trp Val

385 390 395 400

ctt gct ccc gtt gaa gaa gaa cct gta cct gaa ttc taa 1239

Leu Ala Pro Val Glu Glu Glu Pro Val Pro Glu Phe

405 410

<210>108

<211>412

<212>PRT

＜213〉Arabidopis thaliana

<400>108

Met Ala Thr Thr Thr Thr Ser Trp Glu Glu Leu Leu Gly Ser Lys Asn

1 5 10 15

Trp Asp Thr Ile Leu Asp Pro Leu Asp Gln Ser Leu Arg Glu Leu Ile

20 25 30

Leu Arg Cys Gly Asp Phe Cys Gln Ala Thr Tyr Asp Ala Phe Val Asn

35 40 45

Asp Gln Asn Ser Lys Tyr Cys Gly Ala Ser Arg Tyr Gly Lys Ser Ser

50 55 60

Phe Phe Asp Lys Val Met Leu Glu Asn Ala Ser Asp Tyr Glu Val Val

65 70 75 80

Asn Phe Leu Tyr Ala Thr Ala Arg Val Ser Leu Pro Glu Gly Leu Leu

85 90 95

Leu Gln Ser Gln Ser Arg Asp Ser Trp Asp Arg Glu Ser Asn Trp Phe

100 105 110

Gly Tyr Ile Ala Val Thr Ser Asp Glu Arg Ser Lys Ala Leu Gly Arg

115 120 125

Arg Glu Ile Tyr Ile Ala Leu Arg Gly Thr Ser Arg Asn Tyr Glu Trp

130 135 140

Val Asn Val Leu Gly Ala Arg Pro Thr Ser Ala Asp Pro Leu Leu His

145 150 155 160

Gly Pro Glu Gln Asp Gly Ser Gly Gly Val Val Glu Gly Thr Thr Phe

165 170 175

Asp Ser Asp Ser Glu Asp Glu Glu Gly Cys Lys Val Met Leu Gly Trp

180 185 190

Leu Thr Ile Tyr Thr Ser Asn His Pro Glu Ser Lys Phe Thr Lys Leu

195 200 205

Ser Leu Arg Ser Gln Leu Leu Ala Lys Ile Lys Glu Leu Leu Leu Lys

210 215 220

Tyr Lys Asp Glu Lys Pro Ser Ile Val Leu Thr Gly His Ser Leu Gly

225 230 235 240

Ala Thr Glu Ala Val Leu Ala Ala Tyr Asp Ile Ala Glu Asn Gly Ser

245 250 255

Ser Asp Asp Val Pro Val Thr Ala Ile Val Phe Gly Cys Pro Gln Val

260 265 270

Gly Asn Lys Glu Phe Arg Asp Glu Val Met Ser His Lys Asn Leu Lys

275 280 285

Ile Leu His Val Arg Asn Thr Ile Asp Leu Leu Thr Arg Tyr Pro Gly

290 295 300

Gly Leu Leu Gly Tyr Val Asp Ile Gly Ile Asn Phe Val Ile Asp Thr

305 310 315 320

Lys Lys Ser Pro Phe Leu Ser Asp Ser Arg Asn Pro Gly Asp Trp His

325 330 335

Asn Leu Gln Ala Met Leu His Val Val Ala Gly Trp Asn Gly Lys Lys

340 345 350

Gly Glu Phe Lys Leu Met Val Lys Arg Ser Ile Ala Leu Val Asn Lys

355 360 365

Ser Cys Glu Phe Leu Lys Ala Glu Cys Leu Val Pro Gly Ser Trp Trp

370 375 380

Val Glu Lys Asn Lys Gly Leu Ile Lys Asn Glu Asp Gly Glu Trp Val

385 390 395 400

Leu Ala Pro Val Glu Glu Glu Pro Val Pro Glu Phe

405 410

<210>109

<211>1230

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(1230)

<400>109

atg caa aca gtg aaa gct ttg agg aga gtg agt gaa ccc tta caa tgg 48

Met Gln Thr Val Lys Ala Leu Arg Arg Val Ser Glu Pro Leu Gln Trp

1 5 10 15

gtt cgg tct gtt tct tat gga aga cgc ttt tct gct ctc cca aac tat 96

Val Arg Ser Val Ser Tyr Gly Arg Arg Phe Ser Ala Leu Pro Asn Tyr

20 25 30

tcc gca tca gat gca gat ttc gaa gac cag gtt ctg gtg gaa gga aaa 144

Ser Ala Ser Asp Ala Asp Phe Glu Asp Gln Val Leu Val Glu Gly Lys

35 40 45

gct aaa tca aga gct gcc att ctc aat aac cca tct tct ctc aat gct 192

Ala Lys Ser Arg Ala Ala Ile Leu Asn Asn Pro Ser Ser Leu Asn Ala

50 55 60

ctt tct gcg cct atg gtt ggt cgg tta aag agg cta tac gaa tca tgg 240

Leu Ser Ala Pro Met Val Gly Arg Leu Lys Arg Leu Tyr Glu Ser Trp

65 70 75 80

gaa gag aac cca gct att tcc ttt gtt ttg atg aag ggt agc ggt aaa 288

Glu Glu Asn Pro Ala Ile Ser Phe Val Leu Met Lys Gly Ser Gly Lys

85 90 95

act ttc tgc tct ggt gca gat gtc ttg tct ctt tat cac tcg atc aat 336

Thr Phe Cys Ser Gly Ala Asp Val Leu Ser Leu Tyr His Ser Ile Asn

100 105 110

gaa gga aat act gaa gaa tct aaa ctc ttt ttc gag aac ttg tac aag 384

Glu Gly Asn Thr Glu Glu Ser Lys Leu Phe Phe Glu Asn Leu Tyr Lys

115 120 125

ttt gta tac ctc caa gga acg tat tta aaa cca aat ata gca ata atg 432

Phe Val Tyr Leu Gln Gly Thr Tyr Leu Lys Pro Asn Ile Ala Ile Met

130 135 140

gat ggt gtg acc atg ggt tgt ggt ggt gga att tca ctt cca ggg atg 480

Asp Gly Val Thr Met Gly Cys Gly Gly Gly Ile Ser Leu Pro Gly Met

145 150 155 160

ttt cgt gtg gct aca gat aaa act gtg ttg gcc cat cca gag gtc caa 528

Phe Arg Val Ala Thr Asp Lys Thr Val Leu Ala His Pro Glu Val Gln

165 170 175

att ggt ttt cat cct gat gca gga gct tcc tat tat ctt tca cgg ctt 576

Ile Gly Phe His Pro Asp Ala Gly Ala Ser Tyr Tyr Leu Ser Arg Leu

180 185 190

cct ggt tat tta ggg gaa tac ttg gct cta acg ggg cag aaa ctt aat 624

Pro Gly Tyr Leu Gly Glu Tyr Leu Ala Leu Thr Gly Gln Lys Leu Asn

195 200 205

ggt gtc gaa atg ata gca tgt ggc ctt gcc acc cac tat tgc tta aac 672

Gly Val Glu Met Ile Ala Cys Gly Leu Ala Thr His Tyr Cys Leu Asn

210 215 220

gcg aga ctt ccg ttg att gaa gag agg att ggt aaa ctg ttg acc gat 720

Ala Arg Leu Pro Leu Ile Glu Glu Arg Ile Gly Lys Leu Leu Thr Asp

225 230 235 240

gat cct gct gtc att gag gat tct ctt gct caa tat ggt gat ctt gtt 768

Asp Pro Ala Val Ile Glu Asp Ser Leu Ala Gln Tyr Gly Asp Leu Val

245 250 255

tac cct gac agt agc agc gta ctg cac aag ata gag ttg att gat aaa 816

Tyr Pro Asp Ser Ser Ser Val Leu His Lys Ile Glu Leu Ile Asp Lys

260 265 270

tat ttt ggg ctt gat acc gtt gaa gaa atc att gaa gct atg gaa aat 864

Tyr Phe Gly Leu Asp Thr Val Glu Glu Ile Ile Glu Ala Met Glu Asn

275 280 285

gaa gct gct aat tcg tgc aat gaa tgg tgc aag aaa act ctc aaa cag 912

Glu Ala Ala Asn Ser Cys Asn Glu Trp Cys Lys Lys Thr Leu Lys Gln

290 295 300

atc aaa gaa gct tca cct ttg agc tta aag att act ttg caa tct ata 960

Ile Lys Glu Ala Ser Pro Leu Ser Leu Lys Ile Thr Leu Gln Ser Ile

305 310 315 320

cga gaa ggt aga ttc caa acc ctt gat caa tgt ctc aca cat gaa tac 1008

Arg Glu Gly Arg Phe Gln Thr Leu Asp Gln Cys Leu Thr His Glu Tyr

325 330 335

cgt ata tcc att tgt gga gtc tca aaa gta gtc tct ggc gac ttt tgc 1056

Arg Ile Ser Ile Cys Gly Val Ser Lys Val Val Ser Gly Asp Phe Cys

340 345 350

gag ggt att cga gcc cgt ttg gta gat aaa gac ttt gct cca aag tgg 1104

Glu Gly Ile Arg Ala Arg Leu Val Asp Lys Asp Phe Ala Pro Lys Trp

355 360 365

gat cct cca cgc cta gaa gat gtg agc aaa gac atg gtg gat tgc tac 1152

Asp Pro Pro Arg Leu Glu Asp Val Ser Lys Asp Met Val Asp Cys Tyr

370 375 380

ttc acg cca gcc tca gag ctc gat gat tca gat tct gag ttg aag ctg 1200

Phe Thr Pro Ala Ser Glu Leu Asp Asp Ser Asp Ser Glu Leu Lys Leu

385 390 395 400

cca aca gct caa cga gag cct tat ttt tga 1230

Pro Thr Ala Gln Arg Glu Pro Tyr Phe

405

<210>110

<211>409

<212>PRT

＜213〉Arabidopis thaliana

<400>110

Met Gln Thr Val Lys Ala Leu Arg Arg Val Ser Glu Pro Leu Gln Trp

1 5 10 15

Val Arg Ser Val Ser Tyr Gly Arg Arg Phe Ser Ala Leu Pro Asn Tyr

20 25 30

Ser Ala Ser Asp Ala Asp Phe Glu Asp Gln Val Leu Val Glu Gly Lys

35 40 45

Ala Lys Ser Arg Ala Ala Ile Leu Asn Asn Pro Ser Ser Leu Asn Ala

50 55 60

Leu Ser Ala Pro Met Val Gly Arg Leu Lys Arg Leu Tyr Glu Ser Trp

65 70 75 80

Glu Glu Asn Pro Ala Ile Ser Phe Val Leu Met Lys Gly Ser Gly Lys

85 90 95

Thr Phe Cys Ser Gly Ala Asp Val Leu Ser Leu Tyr His Ser Ile Asn

100 105 110

Glu Gly Asn Thr Glu Glu Ser Lys Leu Phe Phe Glu Asn Leu Tyr Lys

115 120 125

Phe Val Tyr Leu Gln Gly Thr Tyr Leu Lys Pro Asn Ile Ala Ile Met

130 135 140

Asp Gly Val Thr Met Gly Cys Gly Gly Gly Ile Ser Leu Pro Gly Met

145 150 155 160

Phe Arg Val Ala Thr Asp Lys Thr Val Leu Ala His Pro Glu Val Gln

165 170 175

Ile Gly Phe His Pro Asp Ala Gly Ala Ser Tyr Tyr Leu Ser Arg Leu

180 185 190

Pro Gly Tyr Leu Gly Glu Tyr Leu Ala Leu Thr Gly Gln Lys Leu Asn

195 200 205

Gly Val Glu Met Ile Ala Cys Gly Leu Ala Thr His Tyr Cys Leu Asn

2l0 215 220

Ala Arg Leu Pro Leu Ile Glu Glu Arg Ile Gly Lys Leu Leu Thr Asp

225 230 235 240

Asp Pro Ala Val Ile Glu Asp Ser Leu Ala Gln Tyr Gly Asp Leu Val

245 250 255

Tyr Pro Asp Ser Ser Ser Val Leu His Lys Ile Glu Leu Ile Asp Lys

260 265 270

Tyr Phe Gly Leu Asp Thr Val Glu Glu Ile Ile Glu Ala Met Glu Asn

275 280 285

Glu Ala Ala Asn Ser Cys Asn Glu Trp Cys Lys Lys Thr Leu Lys Gln

290 295 300

Ile Lys Glu Ala Ser Pro Leu Ser Leu Lys Ile Thr Leu Gln Ser Ile

305 310 315 320

Arg Glu Gly Arg Phe Gln Thr Leu Asp Gln Cys Leu Thr His Glu Tyr

325 330 335

Arg Ile Ser Ile Cys Gly Val Ser Lys Val Val Ser Gly Asp Phe Cys

340 345 350

Glu Gly Ile Arg Ala Arg Leu Val Asp Lys Asp Phe Ala Pro Lys Trp

355 360 365

Asp Pro Pro Arg Leu Glu Asp Val Ser Lys Asp Met Val Asp Cys Tyr

370 375 380

Phe Thr Pro Ala Ser Glu Leu Asp Asp Ser Asp Ser Glu Leu Lys Leu

385 390 395 400

Pro Thr Ala Gln Arg Glu Pro Tyr Phe

405

<210>111

<211>1497

<212>DNA

＜213〉Arabidopis thaliana

<220>

<221>CDS

<222>(1)..(1497)

<400>111

atg cca gga gga tac aaa gcg ttt gag atc tgt gcc aag ttt tgc tat 48

Met Pro Gly Gly Tyr Lys Ala Phe Glu Ile Cys Ala Lys Phe Cys Tyr

1 5 10 15

ggg atg act gtt acg ctc aat gct tac aac ata acc gcg gtg cga tgt 96

Gly Met Thr Val Thr Leu Asn Ala Tyr Asn Ile Thr Ala Val Arg Cys

20 25 30

gca gct gag tat ctt gaa atg act gaa gat gct gac cgc ggt aac ctc 144

Ala Ala Glu Tyr Leu Glu Met Thr Glu Asp Ala Asp Arg Gly Asn Leu

35 40 45

ata tac aag atc gaa gtt ttc ctc aac tca ggc ata ttc aga agc tgg 192

Ile Tyr Lys Ile Glu Val Phe Leu Asn Ser Gly Ile Phe Arg Ser Trp

50 55 60

aaa gac tca atc att gtg ctt cag aca aca aga tct ctt ctt cct tgg 240

Lys Asp Ser Ile Ile Val Leu Gln Thr Thr Arg Ser Leu Leu Pro Trp

65 70 75 80

tct gaa gat ctg aag ctt gtt ggt aga tgc ata gat tct gtt tca gct 288

Ser Glu Asp Leu Lys Leu Val Gly Arg Cys Ile Asp Ser Val Ser Ala

85 90 95

aag atc ttg gtg aac cct gag act atc act tgg tct tat aca ttc aac 336

Lys Ile Leu Val Asn Pro Glu Thr Ile Thr Trp Ser Tyr Thr Phe Asn

100 105 110

agg aag tta tct gga cct gat aag ata gtc gaa tat cat cgg gag aag 384

Arg Lys Leu Ser Gly Pro Asp Lys Ile Val Glu Tyr His Arg Glu Lys

115 120 125

aga gaa gag aat gtg att ccg aaa gat tgg tgg gtc gaa gat gta tgt 432

Arg Glu Glu Asn Val Ile Pro Lys Asp Trp Trp Val Glu Asp Val Cys

130 135 140

gag cta gag att gat atg ttc aag agg gtg ata agt gtt gtg aaa tct 480

Glu Leu Glu Ile Asp Met Phe Lys Arg Val Ile Ser Val Val Lys Ser

145 150 155 160

agt gga agg atg aat aat ggc gta att gct gaa gct ctt aga tac tat 528

Ser Gly Arg Met Asn Asn Gly Val Ile Ala Glu Ala Leu Arg Tyr Tyr

165 170 175

gtt gca agg tgg tta cca gaa tct atg gag tct ttg aca tca gaa gct 576

Val Ala Arg Trp Leu Pro Glu Ser Met Glu Ser Leu Thr Ser Glu Ala

180 185 190

tct tca aac aaa gat ctc gtt gag acg gtt gtt ttc ttg ttg ccg aag 624

Ser Ser Asn Lys Asp Leu Val Glu Thr Val Val Phe Leu Leu Pro Lys

195 200 205

gta aac aga gca atg agc tac tct tct tgc agc ttc ttg cta aaa ctc 672

Val Asn Arg Ala Met Ser Tyr Ser Ser Cys Ser Phe Leu Leu Lys Leu

210 215 220

ctt aaa gtt tcg atc ttg gtt gga gct gat gag acg gtg aga gaa gat 720

Leu Lys Val Ser Ile Leu Val Gly Ala Asp Glu Thr Val Arg Glu Asp

225 230 235 240

ttg gtt gag aac gtg agt ttg aag ctt cat gaa gcg tcc gtt aaa gat 768

Leu Val Glu Asn Val Ser Leu Lys Leu His Glu Ala Ser Val Lys Asp

245 250 255

ttg ctg atc cat gaa gtc gaa tta gtc cat cgg att gtt gat cag ttc 816

Leu Leu Ile His Glu Val Glu Leu Val His Arg Ile Val Asp Gln Phe

260 265 270

atg gcg gat gag aaa cgt gta tct gaa gat gac cgg tac aag gag ttt 864

Met Ala Asp Glu Lys Arg Val Ser Glu Asp Asp Arg Tyr Lys Glu Phe

275 280 285

gtt tta gga aat gga att ttg ttg agt gta gga aga ttg att gat gct 912

Val Leu Gly Asn Gly Ile Leu Leu Ser Val Gly Arg Leu Ile Asp Ala

290 295 300

tat ctc gct ctt aac tct gaa ctt aca ctc tct agc ttt gtt gag tta 960

Tyr Leu Ala Leu Asn Ser Glu Leu Thr Leu Ser Ser Phe Val Glu Leu

305 310 315 320

tct gag tta gtc ccg gaa tca gct agg ccg ata cac gac ggt ctc tac 1008

Ser Glu Leu Val Pro Glu Ser Ala Arg Pro Ile His Asp Gly Leu Tyr

325 330 335

aaa gcc att gac act ttc atg aag gaa cat ccc gaa cta aca aaa tcc 1056

Lys Ala Ile Asp Thr Phe Met Lys Glu His Pro Glu Leu Thr Lys Ser

340 345 350

gaa aag aag agg ctt tgt ggg tta atg gac gtg agg aaa ctg aca aat 1104

Glu Lys Lys Arg Leu Cys Gly Leu Met Asp Val Arg Lys Leu Thr Asn

355 360 365

gaa gca tca acg cac gct gca cag aac gag aga ctt cca cta cga gtg 1152

Glu Ala Ser Thr His Ala Ala Gln Asn Glu Arg Leu Pro Leu Arg Val

370 375 380

gtg gtg caa gtt ctc tac ttt gag cag ctc cga gca aat cac agc ccc 1200

Val Val Gln Val Leu Tyr Phe Glu Gln Leu Arg Ala Asn His Ser Pro

385 390 395 400

gtg gcg tct gtt gcg gct tcg tca cac tcg ccg gtt gag aag acg gag 1248

Val Ala Ser Val Ala Ala Ser Ser His Ser Pro Val Glu Lys Thr Glu

405 410 415

gag aac aaa gga gaa gaa gcg acg aag aag gtg gag ctg agc aag aaa 1296

Glu Ash Lys Gly Glu Glu Ala Thr Lys Lys Val Glu Leu Ser Lys Lys

420 425 430

agc aga gga agc aag age acg agg agt ggt ggt ggt gca cag etg atg 1344

Ser Arg Gly Ser Lys Ser Thr Arg Ser Gly Gly Gly Ala Gln Leu Met

435 440 445

ccg tcg agg tca agg agg atc ttt gag aag ata tgg cct ggg aaa gga 1392

Pro Ser Arg Ser Arg Arg Ile Phe Glu Lys Ile Trp Pro Gly Lys Gly

450 455 460

gag att agc aac aag agc tct gag gtt tct tct gga agc tca caa agt 1440

Glu Ile Ser Asn Lys Ser Ser Glu Val Ser Ser Gly Ser Ser Gln Ser

465 470 475 480

ccg cca gcc aag tct tct agc tcg tct tcc cga cgc cgc aga cat tcg 1488

Pro Pro Ala Lys Ser Ser Ser Ser Ser Ser Arg Arg Arg Arg His Ser

485 490 495

ata tcg tga 1497

Ile Ser

<210>112

<211>498

<212>PRT

＜213〉Arabidopis thaliana

<400>112

Met Pro Gly Gly Tyr Lys Ala Phe Glu Ile Cys Ala Lys Phe Cys Tyr

1 5 10 15

Gly Met Thr Val Thr Leu Asn Ala Tyr Asn Ile Thr Ala Val Arg Cys

20 25 30

Ala Ala Glu Tyr Leu Glu Met Thr Glu Asp Ala Asp Arg Gly Asn Leu

35 40 45

Ile Tyr Lys Ile Glu Val Phe Leu Asn Ser Gly Ile Phe Arg Ser Trp

50 55 60

Lys Asp Ser Ile Ile Val Leu Gln Thr Thr Arg Ser Leu Leu Pro Trp

65 70 75 80

Ser Glu Asp Leu Lys Leu Val Gly Arg Cys Ile Asp Ser Val Ser Ala

85 90 95

Lys Ile Leu Val Asn Pro Glu Thr Ile Thr Trp Ser Tyr Thr Phe Asn

100 105 110

Arg Lys Leu Ser Gly Pro Asp Lys Ile Val Glu Tyr His Arg Glu Lys

115 120 125

Arg Glu Glu Asn Val Ile Pro Lys Asp Trp Trp Val Glu Asp Val Cys

130 135 140

Glu Leu Glu Ile Asp Met Phe Lys Arg Val Ile Ser Val Val Lys Ser

145 150 155 160

Ser Gly Arg Met Asn Asn Gly Val Ile Ala Glu Ala Leu Arg Tyr Tyr

165 170 175

Val Ala Arg Trp Leu Pro Glu Ser Met Glu Ser Leu Thr Ser Glu Ala

180 185 190

Ser Ser Asn Lys Asp Leu Val Glu Thr Val Val Phe Leu Leu Pro Lys

195 200 205

Val Asn Arg Ala Met Ser Tyr Ser Ser Cys Ser Phe Leu Leu Lys Leu

210 215 220

Leu Lys Val Ser Ile Leu Val Gly Ala Asp Glu Thr Val Arg Glu Asp

225 230 235 240

Leu Val Glu Asn Val Ser Leu Lys Leu His Glu Ala Ser Val Lys Asp

245 250 255

Leu Leu Ile His Glu Val Glu Leu Val His Arg Ile Val Asp Gln Phe

260 265 270

Met Ala Asp Glu Lys Arg Val Ser Glu Asp Asp Arg Tyr Lys Glu Phe

275 280 285

Val Leu Gly Asn Gly Ile Leu Leu Ser Val Gly Arg Leu Ile Asp Ala

290 295 300

Tyr Leu Ala Leu Asn Ser Glu Leu Thr Leu Ser Ser Phe Val Glu Leu

305 310 315 320

Ser Glu Leu Val Pro Glu Ser Ala Arg Pro Ile His Asp Gly Leu Tyr

325 330 335

Lys Ala Ile Asp Thr Phe Met Lys Glu His Pro Glu Leu Thr Lys Ser

340 345 350

Glu Lys Lys Arg Leu Cys Gly Leu Met Asp Val Arg Lys Leu Thr Asn

355 360 365

Glu Ala Ser Thr His Ala Ala Gln Asn Glu Arg Leu Pro Leu Arg Val

370 375 380

Val Val Gln Val Leu Tyr Phe Glu Gln Leu Arg Ala Asn His Ser Pro

385 390 395 400

Val Ala Ser Val Ala Ala Ser Ser His Ser Pro Val Glu Lys Thr Glu

405 410 415

Glu Asn Lys Gly Glu Glu Ala Thr Lys Lys Val Glu Leu Ser Lys Lys

420 425 430

Ser Arg Gly Ser Lys Ser Thr Arg Ser Gly Gly Gly Ala Gln Leu Met

435 440 445

Pro Ser Arg Ser Arg Arg Ile Phe Glu Lys Ile Trp Pro Gly Lys Gly

450 455 460

Glu Ile Ser Asn Lys Ser Ser Glu Val Ser Ser Gly Ser Ser Gln Ser

465 470 475 480

Pro Pro Ala Lys Ser Ser Ser Ser Ser Ser Arg Arg Arg Arg His Ser

485 490 495

Ile Ser

<210>113

<211>8045

<212>DNA

＜213〉artificial

<220>

＜223〉carrier

<400>113

actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc agtgcagccg 60

tcttctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc cgccctgccc 120

ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac ttgcgactag 180

aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg ctatgcccgc 240

gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc ggccggctgc 300

accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga gctggccagg 360

atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc 420

cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg 480

cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat ggtgttgacc 540

gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac ccggagcggg 600

cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc gccctaccct caccccggca 660

cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa agaggcggct 720

gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg 780

acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac 840

gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg caccaggacg 900

gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc gcggccgggt 960

acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt 1020

tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa accgagcgcc 1080

gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt 1140

atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt 1200

acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag cccgcgccct 1260

gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga 1320

ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat 1380

tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca 1440

ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca 1500

gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta agcagcgcat 1560

tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac 1620

gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca ttcttgagtc 1680

ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa ccgttcttga 1740

atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc 1800

aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt 1860

gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg 1920

ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg 1980

tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat cgcgcagcta 2040

ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg 2100

catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac 2160

gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg gcactggaac 2220

ccccaagccc gaggaatcgg cgtgacggtc gcaaaccatc cggcccggta caaatcggcg 2280

cggcgctggg tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac 2340

gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc ggccgctgat cgaatccgca 2400

aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat taggaagccg cccaagggcg 2460

acgagcaacc agattttttc gttccgatgc tctatgacgt gggcacccgc gatagtcgca 2520

gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga 2580

tccgctacga gcttccagac gggcacgtag aggtttccgc agggccggcc ggcatggcca 2640

gtgtgtggga ttacgacctg gtactgatgg cggtttccca tctaaccgaa tccatgaacc 2700

gataccggga agggaaggga gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg 2760

tactcaagtt ctgccggcga gccgatggcg gaaagcagaa agacgacctg gtagaaacct 2820

gcattcggtt aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc 2880

gcctggtgac ggtatccgag ggtgaagcct tgattagccg ctacaagatc gtaaagagcg 2940

aaaccgggcg gccggagtac atcgagatcg agctagctga ttggatgtac cgcgagatca 3000

cagaaggcaa gaacccggac gtgctgacgg ttcaccccga ttactttttg atcgatcccg 3060

gcatcggccg ttttctctac cgcctggcac gccgcgccgc aggcaaggca gaagccagat 3120

ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg agagttcaag aagttctgtt 3180

tcaccgtgcg caagctgatc gggtcaaatg acctgccgga gtacgatttg aaggaggagg 3240

cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat 3300

ccgccggttc ctaatgtacg gagcagatgc tagggcaaat tgccctagca ggggaaaaag 3360

gtcgaaaagg tctctttcct gtggatagca cgtacattgg gaacccaaag ccgtacattg 3420

ggaaccggaa cccgtacatt gggaacccaa agccgtacat tgggaaccgg tcacacatgt 3480

aagtgactga tataaaagag aaaaaaggcg atttttccgc ctaaaactct ttaaaactta 3540

ttaaaactct taaaacccgc ctggcctgtg cataactgtc tggccagcgc acagccgaag 3600

agctgcaaaa agcgcctacc cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc 3660

ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct acggccaggc aatctaccag 3720

ggcgcggaca agccgcgccg tcgccactcg accgccggcg cccacatcaa ggcaccctgc 3780

ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc 3840

acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt 3900

gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact 3960

ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa 4020

taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca 4080

ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 4140

taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 4200

agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 4260

cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 4320

tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 4380

tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 4440

gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 4500

acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 4560

acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 4620

cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 4680

gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 4740

gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 4800

agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 4860

ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgcat tctaggtact 4920

aaaacaattc atccagtaaa atataatatt ttattttctc ccaatcaggc ttgatcccca 4980

gtaagtcaaa aaatagctcg acatactgtt cttccccgat atcctccctg atcgaccgga 5040

cgcagaaggc aatgtcatac cacttgtccg ccctgccgct tctcccaaga tcaataaagc 5100

cacttacttt gccatctttc acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga 5160

caagttcctc ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc ggatctttaa 5220

atggagtgtc ttcttcccag ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt 5280

aatccaattc ggctaagcgg ctgtctaagc tattcgtata gggacaatcc gatatgtcga 5340

tggagtgaaa gagcctgatg cactccgcat acagctcgat aatcttttca gggctttgtt 5400

catcttcata ctcttccgag caaaggacgc catcggcctc actcatgagc agattgctcc 5460

agccatcatg ccgttcaaag tgcaggacct ttggaacagg cagctttcct tccagccata 5520

gcatcatgtc cttttcccgt tccacatcat aggtggtccc tttataccgg ctgtccgtca 5580

tttttaaata taggttttca ttttctccca ccagcttata taccttagca ggagacattc 5640

cttccgtatc ttttacgcag cggtattttt cgatcagttt tttcaattcc ggtgatattc 5700

tcattttagc catttattat ttccttcctc ttttctacag tatttaaaga taccccaaga 5760

agctaattat aacaagacga actccaattc actgttcctt gcattctaaa accttaaata 5820

ccagaaaaca gctttttcaa agttgttttc aaagttggcg tataacatag tatcgacgga 5880

gccgattttg aaaccgcggt gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca 5940

tgctaccctc cgcgagatca tccgtgtttc aaacccggca gcttagttgc cgttcttccg 6000

aatagcatcg gtaacatgag caaagtctgc cgccttacaa cggctctccc gctgacgccg 6060

tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg 6120

agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac gcttagacaa 6180

cttaataaca cattgcggac gtttttaatg tactgaatta acgccgaatt actagatatc 6240

gatttggtgt atcgagattg gttatgaaat tcagatgcta gtgtaatgta ttggtaattt 6300

gggaagatat aataggaagc aaggctattt atccatttct gaaaaggcga aatggcgtca 6360

ccgcgagcgt cacgcgcatt ccgttcttgc tgtaaagcgt tgtttggtac acttttgact 6420

agcgaggctt ggcgtgtcag cgtatctatt caaaagtcgt taatggctgc ggatcaagaa 6480

aaagttggaa tagaaacaga atacccgcga aattcaggcc cggttgccat gtcctacacg 6540

ccgaaataaa cgaccaaatt agtagaaaaa taaaaactga ctcggatact tacgtcacgt 6600

cttgcgcact gatttgaaaa atctcaatat aaacaaagac ggccacaaga aaaaaccaaa 6660

acaccgatat tcattaatct tatctagttt ctcaaaaaaa ttcatatctt ccacacgtgg 6720

atccgtcgag tctaccatga gcccagaacg acgcccggcc gacatccgcc gtgccaccga 6780

ggcggacatg ccggcggtct gcaccatcgt caaccactac atcgagacaa gcacggtcaa 6840

cttccgtacc gagccgcagg aaccgcagga gtggacggac gacctcgtcc gtctgcggga 6900

gcgctatccc tggctcgtcg ccgaggtgga cggcgaggtc gccggcatcg cctacgcggg 6960

cccctggaag gcacgcaacg cctacgactg gacggccgag tcgaccgtgt acgtctcccc 7020

ccgccaccag cggacgggac tgggctccac gctctacacc cacctgctga agtccctgga 7080

ggcacagggc ttcaagagcg tggtcgctgt catcgggctg cccaacgacc cgagcgtgcg 7140

catgcacgag gcgctcggat atgccccccg cggcatgctg cgggcggccg gcttcaagca 7200

cgggaactgg catgacgtgg gtttctggca gctggacttc agcctgccgg taccgccccg 7260

tccggtcctg cccgtcaccg agatttgact cgaccggcat gccctgcttt aatgagatat 7320

gcgagacgcc tatgatcgca tgatatttgc tttcaattct gttgtgcacg ttgtaaaaaa 7380

cctgagcatg tgtagctcag atccttaccg ccggtttcgg ttcattctaa tgaatatatc 7440

acccgttact atcgtatttt tatgaataat attctccgtt caatttactg attgtccaag 7500

cttaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct 7560

cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg 7620

attacgaatt cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc 7680

ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt 7740

aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc 7800

gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gctagagcag cttgagcttg 7860

gatcagattg tcgtttcccg ccttcagttt aaactatcag tgtttgacag gatatattgg 7920

cgggtaaacc taagagaaaa gagcgtttat tagaataacg gatatttaaa agggcgtgaa 7980

aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc cctcgggatc 8040

aaagt 8045

At1g61800，SEQ.ID No.1

＞GM59659543 glucose-6 phosphoric acid/phosphoric acid transposition amyloid protein precursor, plastid

atgatctcttcgttgagacagcctgttgtagggatcagtggttctgatcttcttttgaggcaaagacatgcaactccaattaaggc

aaggtcgtttttaccctctttgtcaagagaaaagggtcaaggatctcttgtttcagttcaaaagccactgcacattggtgcttctct

tggtgttggaaattttgtgtcagtgaagagtgatgataaaaggggtgatttggtgaagtgtgaggcctatgaggcagacagat

cagaggttgagggtgcaagcacaccgtcagaggctgcaaagaaggtgaaaattgggatatattttgcaacctggtgggct

ctgaatgtggtgttcaatatttataacaagaaggttctgaatgcctacccttacccttggcttacttcaactctctcacttgcatgtg

ggtctcttatgatgttgatctcttgggccactgggattgcagaagcccccaagactgatcctgagttttggaagtctttgttccctg

ttgctgttgcacatacaataggacatgtagcggcaacagttagtatgtcaaaagttgcggtatcgtttacacatattatcaaga

gtggtgaacctgcttttagtgttctggtttcaagatttcttttgggtgagagcttccctgtgcctgtctatctgtctttaattccaatcatt

ggtggatgtgcacttgctgctgtgactgagctcaatttcaatatgatcggttttatgggagctatgatatcgaatttggcatttgtatt

ccgtaatatcttttccaaaaagggcatgaagggaaagtctgtcagtggaatgaattactatgcttgtttatctattttgtcccttgc

aattctcacacccttcgcaattgctgtggaaggaccacagatgtgggctgctggatggcaaacagctatgtctcaaattgga

ccccaattcatatggtgggtagctgctcaaagtgtattctatcatctatacaatcaagtgtcgtacatgtctctggatcagatctct

cccttgacgtttagcattggaaacaccatgaaacgtatatctgtcattgtgtcttccattattatcttccacacaccagttcaaccc

atcaatgctcttggtgctgccattgctatccttggaaccttcttgtattcacaggcgaaacaatag

＞GM59659543 Robison ester/phosphoric acid transposition amyloid protein precursor, plastid

misslrqpvvgisgsdlllrqrhatpikarsflpslsrekgqgslvsvqkplhigaslgvgnfvsvksddkrgdlvkceayeadrs

evegastpseaakkvkigiyfatwwalnvvfniynkkvlnaypypwltstlslacgslmmliswatgiaeapktdpefwkslf

pvavahtighvaatvsmskvavsfthiiksgepafsvlvsrfllgesfpvpvylslipiiggcalaavtelnfnmigfmgamisnl

afvfrnifskkgmkgksvsgmnyyaclsilslailtpfaiavegpqmwaagwqtamsqigpqfiwwvaaqsvfyhlynqvs

ymsldqispltfsigntmkrisvivssiiifhtpvqplnalgaalailgtflysqakq

＞KO07221 arabidopsis thaliana chromosome | BAC F8K4 sequence, sufficient sequence

ATGCTTTCTTCAATCAAACCATCTTCGTCTTCCTTCTCCACCGCTAATCTCCGGCAGCGTAAGG

CGGTCAA

TTCCTACAAAGCTCAAGTTTTCTCCTTTACTCATCATCAAAAACTGCCATAACCAAAGCTTTAA

CGCTAA

TGTTGTCTCCCATCAAAAGCCTCTACACATTTCATCTGCTTCGAATTTCAAGCGTGAGGTCAA

AGTCGAA

GCTTACGAGGCCGATCGTTCCCGTCCACTGGACATCAACATCGAGCTTCCCGATGAACAATC

CGCGCAGA

AACTGAAAATCGGAATCTACTTCGCAACTTGGTGGGCACTTAACGTTGTCTTCAACATCTACA

ACAAGAA

AGTCCTCAATGCTTTTCCTTACCCGTGGCTTACTTCGACGTTGTCTCTCGCTTGTGGTTCTTT

GATGATG

CTTGTCTCTTGGGCTACTAGAATCGCAGATGCTCCTAAAACTGATCTCGAGTTCTGGAAAACT

CTGTTCC

CGGTCGCTGTAGCACACACGATAGGACACGTTGCAGCAACAGTGAGTATGTCAAAAGTAGC

AGTTTCCTT

CACACACATCATTAAAAGTGGTGAACCAGCTTTCAGTGTCTTAGTCTCAAGATTCTTCATGGG

AGAGACT

TTCCCTCTTCCTGTCTATCTCTCTCTCTTACCAATCATCGGAGGCTGCGCTCTCGCGGCCATC

ACCGAGC

TTAACTTCAACATCACTGGGTTTATGGGGGCAATGATATCGAATTTGGCATTTGTGTTCCGGA

ATATCTT

TTCGAAGAAAGGGATGAAAGGGAAGTCAGTGAGCGGAATGAACTACTACGCTTGCTTATCGA

TGATGTCT

CTTGTGATCCTCACTCCATTTTCTATTGCCGTGGAAGGTCCTCAAATGTGGGCTGCTGGTTG

GCAAAATG

CGGTTTCTCAAGTCGGACCAAACTTTGTCTGGTGGGTAGTGGCACAAAGTGTGTTTTACCAT

TTGTACAA

TCAGGTCTCATACATGTCATTAGACCAGATTTCGCCGTTAACTTTCAGTATCGGTAATACGAT

GAAGCGG

ATTTCCGTTATTGTTGCATCGATCATCATTTTCCATACCCCGATTCAACCGGTTAATGCCCTC

GGTGCTG

CCATTGCGATTTTTGGAACTTTCCTCTACTCACAGGTAAACAAACAAACTTATTCTTGTTTACC

TACCTC

TAGTACTTGGTTCTTTAGTGAACAATTTCTAGTTCTAATATAA

＞KO07221 gi|3367515|gb|AAC28500.1| is similar to Robison ester/phosphoric acid transposition albumen (GPT)

(GPT) gb|AF020814 from Pisum sativum.[Arabidopis thaliana]

MLSSIKPSSSSFSTAISGSVRRSIPTKLKFSPLLIIKNCHNQSFNANVVSHQKPLHISSASN

FKREVKVE

AYEADRSRPLDINIELPDEQSAQKLKIGIYFATWWALNVVFNIYNKKVLNAFPYPWLTSTL

SLACGSLMM

LVSWATRIADAPKTDLEFWKTLFPVAVAHTIGHVAATVSMSKVAVSFTHIIKSGEPAFSVL

VSRFFMGET

FPLPVYLSLLPIIGGCALAAITELNFNITGFMGAMISNLAFVFRNIFSKKGMKGKSVSGMN

YYACLSMMS

LVILTPFSIAVEGPQMWAAGWQNAVSQVGPNFVWWVVAQSVFYHLYNQVSYMSLDQI

SPLTFSIGNTMKR

ISVIVASIIIFHTPIQPVNALGAAIAIFGTFLYSQVNKQTYSCLPTSSTWFFSEQFLVLI

＞GM59772786 is similar to Robison ester/phosphoric acid transposition albumen

atgatctcttcaatgaagtgcacagcctcatctctcacatgttccgctttctcaaacagaaagattcccattgcaaggccacaa

cttgtcacgttaccaaccattaacaacgttgaacaaaacacgggtctctctcagttgtgttcacagaaaccgctctacctttcat

ccactgagaacttggcattggtgaagagaaggagggagactgagtgccaggcctatgaagccgataggtcacggcctct

ggagattaacattgagctgccgggcgaagaggcggcgcagaggttcaagattgggctgtattttgctacatggtgggctttg

aatgtggtgttcaacatttacaacaagaaggttttgaatgcttttccttacccatggctcacctccactttgtcccttgctgctggct

ccctcatgatgttagtctcctgggccaccagggttgctgaggtcccaaaagttaacttggacttctggaaggccttgtttcctgtt

gcggtggcacacacaattgggcatgttgctgcaactgtgagcatgtccaaggttgctgtttcattcactcacatcatcaagagt

ggagagccagctttcagtgttcttgtgtcaaggttcttgctcggagaagcattcccaatgccggtatatctgtcactgctgccaat

tattggtggttgtgcattagctgcagtgactgagctcaatttcaatatgattggctttatgggggctatgatatcaaatttggcattc

gtcttccgcaatatattctcaaagaaagggatgaagggaatgtctgttagtggaatgaactactatgcttgtctttccataatgtc

actactaattctcacaccttttgccattgctgttgagggccccaaggtttggattgcaggctggcaaacagctgtgtctcagattg

gtcccaattttgtatggtgggtagctgcccagagtgtcttctaccacttgtacaatcaagtctcatacatgtctcttgatcagatttct

cccttaacattcagcattggaaacacaatgaagaggatttcggtcattgtctcttccatccttatctttcacacgcctgttcagccc

atcaatgctcttggtgctgccattgcaattcttggcaccttcctctattcacaggctaaacagtaa

＞GM59772786 is similar to Robison ester/phosphoric acid transposition albumen

mlssmkctassltcsafsnrkipiarpqlvtlptinnveqntglsqlcsqkplylsstenlalvkrrretecqayeadrsrpleiniel

pgeeaaqrfkiglyfatwwalnvvfniynkkvlnafpypwltstlslaagslmmlvswatrvaevpkvnldfwkalfpvavaht

lghvaatvsmskvavsfthiiksgepafsvlvsrfllgeafpmpvylsllpiiggcalaavtelnfnmigfmgamisnlafvfmif

skkgmkgmsvsgmnyyaclsimslliltpfaiavegpkvwiagwqtavsqlgpnfvwwvaaqsvfyhlynqvsymsldq

ispltfsigntmkrisvivssilifhtpvqpinalgaalailgtflysqakq*

At1g61810，SEQ ID No.3

＞KO07221 arabidopsis thaliana chromosome 1BAC F8K4 sequence, sufficient sequence

ATGAAAAATTTAACCAGTTTCGTAATTGTTATCTTACTACAAAGCTTATTGTTTCATGTATATGG

TCGTC

ATCAAAGTTCTTCGAAGAACATTTTAGTAGATTCATCTCCATTTCCTTCTGATTTCCTCTTTGG

TACAGC

TTCTTCCGCTTACCAGTATGAAGGTGCGTTCTTGACTGATGGAAAAAGTTTGAACAATTGGGA

TGTCTTT

ACGCATAAAAATCCTGGGAAAATACTTGACAAGAACAATGCAGATAGAGCTGTGGACCAATA

TAATCGAT

TTTTGGAGGACATCCAATTAATGTCTTTTCTTGGAGTGAACAGTTATAGGTTTTCAATTTCTTG

GTGTAG

AATCTTACCTAGAGGAAGATTTGGAGAGATTAATTATTTGGGGATAAAGTATTACAACATATTT

ATCGAT

GCTCTCATTAGTAGAGGGATTAAACCATTTGTGACGTTGAACCATGTCGACTATCCTCAAGAA

CTCGAGG

ACCGGTTTCAAAGTTGGTTAAACCCCGAGATGCAGAAGGAATTTGGTTATTTAGCCGATATAT

GTTTCAA

GCATTTTGGAAACCGAGTTAAATACTGGACCACGCTTAACGAACCAAATCAACAATTAATCTT

AGGCTAT

CTAACAGGTAAATTTCCACCCTCTCGTTGCTCCTCGCCATACGGGAACTGTAGCCAGGGGAA

TTCAGAAA

CCGAACCTTTCATAGCCGCACATAACATGATCCTTGCACACGCAAAAGCGGTTAACATATACA

AGACCAA

ATATCAGAAAGAACAAAAGGGAAGCATTGGCATTGTGGTACAAACATCATGGTTTGAACCCAT

TAGTGAT

TCCAATGCGGATAAAGAAGCTGCTGAGAGAGCTCAATCATTTTACTCTAATTGGATTCTAGAT

CCCGTTA

TATATGGGAAATATCCAAAAGAAATGGTAGATATTCTTGGACCAGCCTTGCCACAATTTTCGA

GCAATGA

AGTGAAGAACTTAGAGAAGTCGAGGGCAGATTTCGTTGGTATTAATCACTATACAAGTTACTT

CATTCAA

GATTGTTTGACCTCTGCTTGTAATACTGGACATGGAGCTTTTAAGGCCGAAGGATATGCTCTC

AAGTTAG

ACCGGAAAGGCAATGTTACTATAGGAGAACTTACCGATGTAAATTGGCAGCATATTGATCCC

ACAGGATT

CCACAAGATGCTGAATTATTTAAAAGATAGGTACCCAAACATGCCAATGTTCATAACTGAAAA

CGGTTTT

GGAGACTTGCAAAAACCTGAAACAACGGATAAAGAACTTCTAAATGATACAAAAAGGATCCAA

TACATGA

GTGGATACTTAGAAGCTTTACAAGCAGCAATGAGGGATGGAGCAAATGTGAAGGGTTATTTT

GTGTGGTC

ACTATTAGACAATTTTGAGTGGTTGTTCGGATACAAGGTTCGGTTTGGTCTATTCCATGTGGA

TCTAACA

ACTCTTAAAAGGTCACCGAAACAATCAGCTTCATGGTACAAAAATTATATCGAAGAACACGTG

AATCGAA

GAGATATCGTAGATAATTATTAA

＞KO07221 gi|3367516|gb|AAC28501.1| is similar to beta-glucosidase enzyme BGQ60 precursor

Gbl L41869 is from barley [Arabidopis thaliana]

MKNLTSFVIVILLQSLLFHVYGRHQSSSKNILVDSSPFPSDFLFGTASSAYQYEGAFLTD

GKSLNNWDVF

THKNPGKILDKNNADRAVDQYNRFLEDIQLMSFLGVNSYRFSISWCRILPRGRFGEINYL

GIKYYNIFID

ALISRGIKPFVTLNHVDYPQELEDRFQSWLNPEMQKEFGYLADICFKHFGNRVKYWTTL

NEPNQQLILGY

LTGKFPPSRCSSPYGNCSQGNSETEPFIAAHNMILAHAKAVNIYKTKYQKEQKGSIGIVV

QTSWFEPISD

SNADKEAAERAQSFYSNWILDPVIYGKYPKEMVDILGPALPQFSSNEVKNLEKSRADFV

GINHYTSYFIQ

DCLTSACNTGHGAFKAEGYALKLDRKGNVTIGELTDVNWQHIDPTGFHKMLNYLKDRY

PNMPMFITENGF

GDLQKPETTDKELLNDTKRIQYMSGYLEALQAAMRDGANVKGYFVWSLLDNFEWLFGY

KVRFGLFHVDLT

TLKRSPKQSASWYKNYIEEHVNRRDIVDNY

At5g65610，SEQ.ID No.5

＞KO07169 gi|18424968|ref|NM_125959.1| arabidopsis thaliana chromosome, 5

The CHR5v07142Q02 genome sequence

ATGGCGACAGAGAGAGAGACGATTGAGAGGAGAGATCAAAGGATTCTCGTTTCCATTGACG

ACGATGGTG

GAGGAAGAAAGCAATCATCAAAGACAAAGAAGCTTCTCAGATTACGAATGAAGAAGACGGAG

AATTTCAG

ACGACGACGACGCAAGGTGGGCTCACGGTGGTGGGCTTTTTTTTATGGGCCCCTTCTTTGCT

ACCAAATC

CAACCAGAAAAAGTTTTATGCTTGAAGCTCTCGTCGGTCGCTAGAGCTGAATTTGATTTGAGG

GAGATGG

AAAGAGACAAGGTTAATGATTTTGTGGTTAATGGCAAGATTAACAAGTTAATTAAGTTATTATC

AAATAG

AGGATCCCATATAGTAACTTTACGAGAGCATAACGTAGACGAACCAACACTAACCACAAAACT

TAGGGGT

GCATACAAAAATAAAATTGAAAAAACAGAGCATAGATCAGTGATCCGGACTCTGTTTTGGAAG

ATCTCTA

AAAAGCTTAGGATCATCATCGGCATCGCGCAGATAGAAACCATCATCGAAATTCTGCGAATA

GCTCACAG

CATCGTAGTGAAACGTCTTGTCTTGTTGTTGTGGTTACTGATTCGAGCGTACTCTGTTTCTTC

TGTATGG

ATGAGACAAGATAACGGCGAACTCAAGCATCGACCGGTGGAGTACGATTGTCTACGGTTGTC

GGCGAGAA

CTCTATCCATTTCTTGCAAAAATAAAAGAGAGATTGTTTTATTGATTGAGTGA

＞KO07169 gi|15239089|ref|NP 201364.1| agnoprotein matter; Protein id:At5g65610.1

[Arabidopis thaliana]

MATERETIERRDQRILVSIDDDGGGRKQSSKTKKLLRLRMKKTENFRRRRRKVGSRWW

AFFYGPLLCYQI

QPEKVLCLKLSSVARAEFDLREMERDKVNDFVVNGKINKLIKLLSNRGSHIVTLREHNVD

EPTLTTKLRG

AYKNKIEKTEHRSVIRTLFWKISKKLRIIIGIAQIETIIEILRIAHSIVVKRLVLLLWLLIRAYSVS

SVW

MRQDNGELKHRPVEYDCLRLSARTLSISCKNKREIVLLIE

At5g65600，SEQ ID NO.7

＞KO07169 gi|18424967|ref|NM_125958.1| arabidopsis thaliana chromosome, 5

The CHR5v07142002 genome sequence

ATGCTTTATTTCATTTTCTGTCAAAATCTTTCTTCTTCCTCCTCTATGTCTAATTCAATCTTGTT

CCTTT

CCCTTTTCCTCTTCCTCCCTTTTGTTGTTGATTCACTTTACTTCAACTTTACAAGTTTTCGACAA

GGTGA

TCCCGGGGACATATTTTACCACGGGGATGCAACTCCCGATGAAGATGGAACAGTGAACTTCA

ACAACGCT

GAACAAACATCTCAGGTTGGTTGGATTACTTATTCAAAGAAGGTGCCTATATGGAGTCATAAA

ACTGGTA

AGGCTTCAGATTTCAGCACCAGTTTCTCCTTCAAAATCGATGCTCGTAATCTTTCAGCAGATG

GTCATGG

AATCTGTTTCTTTCTTGCTCCTATGGGAGCTCAACTACCTGCATACTCAGTTGGTGGTTTCTT

GAATTTG

TTCACTCGGAAGAATAATTACTCATCTTCGTTTCCGCTAGTTCATGTTGAATTTGACACGTTTA

ACAATC

CAGGATGGGATCCTAATGATGTTGGATCTCATGTTGGGATCAATAACAACTCTCTTGTTTCTT

CCAACTA

CACTTCTTGGAATGCAAGCTCTCACAGCCAAGATATTTGTCATGCAAAGATCTCTTATGATTC

CGTGACC

AAGAATTTAAGTGTGACTTGGGCTTATGAGCTAACAGCAACCTCTGATCCTAAGGAGAGTTCA

AGCCTTT

CTTACATCATCGACCTCGCTAAGGTTCTGCCATCAGATGTTATGTTCGGGTTTATAGCTGCCG

CTGGGAC

AAACACAGAGGAACATAGACTTTTATCATGGGAGCTCAGTTCAAGTTTGGACAGCGACAAAG

CCGATAGC

AGGATAGGACTAGTAATTGGCATTTCGGCTTCCGGGTTTGTTTTTCTGACGTTTATGGTCATC

ACAACCG

TGGTGGTTTGGTCACGGAAGCAAAGAAAGAAGAAAGAAAGAGAATAGAAAACATGATATCG

ATAAACAA

AGACCTTGAAAGGGAAGCAGGACCAAGAAAGTTTTCTTATAAGGATCTTGTGTCAGCAACCA

ACAGATTC

TCAAGCCATAGAAAGCTAGGTGAAGGAGGATTTGGAGCAGTTTATGAAGGAAACTTGAAAGA

AATCAATA

CAATGGTTGCGGTGAAGAAACTATCCGGTGATTCAAGGCAGGGAAAGAACGAGTTTTTAAAC

GAAGTTAA

GATCATCAGCAAACTGAGACATCGAAACCTCGTGCAGCTCATCGGTTGGTGTAATGAGAAAA

ATGAGTTC

TTGCTGATATATGAGTTAGTTCCAAATGGTAGCTTGAACTCTCACCTCTTTGGGAAAAGACCC

AATTTAC

TTTCTTGGGACATAAGGTACAAGATAGGTCTTGGTTTAGCCTCTGCTCTGCTTTATCTTCATG

AAGAATG

GGATCAATGTGTACTGCACAGAGACATCAAGGCAAGTAATATAATGCTTGACAGTGAATTCAA

TGTAAAG

TTAGGTGATTTCGGGTTGGCTCGGCTAATGAACCATGAACTTGGTTCCCATACAACGGGGTT

GGCTGGAA

CTTTTGGGTACATGGCACCTGAATATGTAATGAAAGGAAGTGCGAGTAAAGAATCTGATATAT

ATAGCTT

TGGAATTGTTTTACTAGAGATTGTCACAGGGAGAAAATCGCTGGAACGTACACAAGAAGATA

ACAGTGAT

ACAGAGAGTGATGATGAGAAGAGTCTAGTGGAGAAAGTTTGGGAGCTTTATGGAAAACAAGA

ACTGATCA

CTTCATGTGTTGATGACAAATTGGGTGAGGATTTCGACAAGAAAGAAGCTGAATGTCTTCTG

GTTTTAGG

GTTATGGTGTGCTCATCCTGATAAAAACTCGAGACCTTCGATAAAACAAGGGATCCAAGTCAT

GAATTTT

GAGTCACCATTGCCTGATCTTCCATTGAAGAGGCCTGTTGCTATGTATTACATCTCGACTACT

ACTTCTT

CTTCTTCTCCCTCAGTTAACTCAAATGGAGTTTCTGTAACGTTCTCAGGCATCGAGTATGGTC

GTTAA

＞KO07169 gi|15239088|ref|NP_201363.1| receptor protein kinase albuminoid; Protein:

At5g65600.1 [Arabidopis thaliana]

MLYFIFCQNLSSSSSMSNSILFLSLFLFLPFVVDSLYFNFTSFRQGDPGDIFYHGDATPD

EDGTVNFNNA

EQTSQVGWITYSKKVPIWSHKTGKASDFSTSFSFKIDARNLSADGHGICFFLAPMGAQL

PAYSVGGFLNL

FTRKNNYSSSFPLVHVEFDTFNNPGWDPNDVGSHVGINNNSLVSSNYTSWNASSHSQ

DICHAKISYDSVT

KNLSVTWAYELTATSDPKESSSLSYIIDLAKVLPSDVMFGFIAAAGTNTEEHRLLSWELS

SSLDSDKADS

RIGLVIGISASGFVFLTFMVITTVVVWSRKQRKKKERDIENMISINKDLEREAGPRKFSYK

DLVSATNRF

SSHRKLGEGGFGAVYEGNLKEINTMVAVKKLSGDSRQGKNEFLNEVKIISKLRHRNLVQ

LIGWCNEKNEF

LLIYELVPNGSLNSHLFGKRPNLLSWDIRYKIGLGLASALLYLHEEWDQCVLHRDIKASNI

MLDSEFNVK

LGDFGLRLMNHELGSHTTGLAGTFGYMAPEYVMKGSASKESDIYSFGIVLLEIVTGRK

SLERTQEDNSD

TESDDEKSLVEKVWELYGKQELITSCVDDKLGEDFDKKEAECLLVLGLWCAHPDKNSR

PSIKQGIQVMNF

ESPLPDLPLKRPVAMYYISTTTSSSSPSVNSNGVSVTFSGIEYGR

At2g22530，SEQ ID No.9

＞KO08273 (gi|20197885) arabidopsis thaliana chromosome 2 clone F14M13 figure

Mi238, complete sequence

ATGACGACGGCGGCGATGACTTGTACGAGACTGACGATATTTACGGTGGCCGGAATATTACT

TCAGATTA

TTGGCCTCTCCATTTTCGTATTCGGTTTCTTCCCCGTCAAGCCGACTCTCTCCGGCGTCAGT

GGCTCGGA

GAGCTATCGAGATCCATTCTGTGATTCTTCTCTGATTTCGAACGAATCGGAGCTTCATCATCC

AGAGAAA

CTGAGATTGCTGTACCAGGTTATTGATGGGCTACCTGCGGAGTTCGTTCTTGGGAAAGATGG

TAAACCTC

CAGAGAAGGTTTTGAAAGAGTCTATGCCTTATACTCAGTCACTGTTAGCTAATGGAGATGCTA

TTGGTTA

CCATGCTAAAGCAGCTCCTCCAACTGTTACCATGCCGAGGTTAAAGGCAATGGTTTCTGGGG

CAATTGGT

GGTTTCTTGGATGTTGCTTTTAATTTTAACACACAAGCTCTCTTAGATGATAATATTCTTGGTC

AGTTTT

TCAGGATTGGTTGGAAAATGGTGATGCTCGGTGATGAGACGTGGCTCAAGTTATTTCCAGGG

CTATTTAT

GAGACATGACGGTGTTAGCAGTTTCTTTGTCAAAGATACAGTACAGGTAGACAGAAATGTTTC

TCGACAC

TTGCCCGATGAGCTAAACAGTGATGATTGGAATCTCTTGATCCTTCATTACCTTGGTTTAGAT

CACGTTG

GACATACTGGCGGCCGTAACAGCCCCTTGATGCCTGCAAAACTTAAAGAAATGGATGATATA

GTTAGAAC

AATGCATTTAAGAGCCATGATGGATCGCAGCCATGATCAAGGACAGACTCTTCTGATAATAGT

CAGTGAT

CATGGCATGACTGAGAATGGAAATCACGGAGGGTCGTCATATGAAGAAACTGACTCCTTAAT

GCTCTTTA

TTGGCTTGAATAGCAATATTTCTGACTATGCTTCAGCTACCAATAATGTAGCTTTCCAGGTAG

ATTTGGC

GCCAACTTTAGCTCTTCTATTTGGTGTGCCGATCCCAAAGAACAATGTTGGAGTCCTTGTCCC

AGGAACA

CTCTGTTCTTTAAGAGATTTTGAGCAACTACGGGCACTAGAACTGAATTCATGGCAGTTACTC

AGACTTA

TGCTAGCACAGATACAAAGTTCTTCGTTTCCACGCGTCTCCTGCAATTGCTTCCTTGATGGAA

CTTGTGA

GGGTCTTGATTTGGATATTAGTGAGTGTTCTGGGGATAAAGAGAAACAACTTATTTGCTTGTT

TAGGAAT

GCTGCTGCCCTCCATGGCATTTGGAAGTCCAAGAAATCAACAGAGTCGTC TAGTACCATGGA

AGATTTCA

GCAGAGCTTTAGATGCATATAACACCTTCTTGAAAACTGCAAGTGAGTGGTTAGCAAGCAAAA

CCACGGA

GAAACCTGTTCTCTTACTCGGTCTTGGAGTGAGTGCCATGCTTCTGTCATGCTTCATCTGTGG

TACTGTC

TTTCTGTCCTTATTCAAAGAGGTTTACCATGAGCCCAAGGATAGGGTCTGCAGTTTGAGTAAT

TTGTTGA

ATTTAGAAGAGGTGTTCATTTTTGCTCTTCTTTTGATACTTGTTATAAGCATGGGATCTAGTTC

AATGGT

GGAAGAAGAGCATTATATATGGCATTTCATGGTATCAACATTCCATCTTCTGTTACTCTTCAAG

ACAGCG

AAGTCATTCAAAATTTCCAAGGGGATGAACATTCTCAGAGACTTTAAATTCGGTTCTATCTTCT

CGCTTC

TTATTTCTGGTAGACTACTGAGAGGTTGGCATCAAGGGGGCGTGAACTGGACTTACCTTCCT

GATATTTC

TAAGTGGCTAGTGCAAGGTGGCAGTGGTTATGTGAAATGGATTCAGCTAATCTCAATAATCCT

AGTTATT

GGTCTAGGACTATATACTCTCTTTCGAACAGGATCGAATAGAAAAGGTGTTCGCATCCTAGCA

TTTGGTT

TCTCAACTTGTGGGTTCCTAGTCTTGCTGCATGTAGGGAGATATCAGGATGAACTGTCAACC

GGTTTTGG

AGCCACTGTCACAGTAAAAGTTATTTATTATCTTCTATCTATATCTTCCATTGGAGCTTCTTTG

GTTTTG

CCATGGTCTGCGCTAAACAAAGACAAGTCCTTTCTAGCTGAAGTAGGTGATTGCTTGTATTTG

ATTGGCT

CTGCGTACATACTTTGCTGGTGTCTTCTACAGCAACTTCTCCAACAACCAATCAACTCGGGCC

CCATACT

TTTGCTGCTCCTCCAAATCTTAGCAATTTTATGTCTTTCTTCTAGTGATTTTCAAGTTAATGAAT

GGGTC

GAGATTACTGCACTCTATTACATGGGAATGGCAGGTCACTTTGCTCTTGGAAACAGCAACAC

TTTAGCAA

CAATCGATGTTGCTGGTGCTTTTATCGGAATCTCTAGTCATTCTACAATACTCTCTGGGATCT

TGATGTT

CATGATCACCTATGCATCTCCCATGCTGTTCCTTCTATCCCTCGTCATGTACATTGGTGCAAA

TCTTAGA

AACCACTCAAACTCAACAATATCAACTCACCGGGAAACGACTAAAGCTCGGGTTCCCTTGCC

TTGTTCCG

CTCTGCATCAACTCAATACTCTTGACCGCATATACTGTGGTCTTACTACTCATGAGAAATCAC

CTCTTTG

TATGGAGCGTCTTCTCTCCCAAGTGAGTCTTTGA

＞KO08273gi|6598615|gb|AAF18652.1| agnoprotein matter [Arabidopis thaliana]

MTTAAMTCTRLTIFTVAGILLQIIGLSIFVFGFFPVKPTLSGVSGSESYRDPFCDSSLISNE

SELHHPEK

LRLLYQVIDGLPAEFVLGKDGKPPEKVLKESMPYTQSLLANGDAIGYHAKAAPPTVTMP

RLKAMVSGAIG

GFLDVAFNFNTQALLDDNILGQFFRIGWKMVMLGDETWLKLFPGLFMRHDGVSSFFVK

DTVQVDRNVSRH

LPDELNSDDWNLLILHYLGLDHVGHTGGRNSPLMPAKLKEMDDIVRTMHLRAMMDRSH

DQGQTLLIIVSD

HGMTENGNHGGSSYEETDSLMLFIGLNSNISDYASATNNVAFQVDLAPTLALLFGVPIPK

NNVGVLVPGT

LCSLRDFEQLRALELNSWQLLRLMLAQIQSSSFPRVSCNCFLDGTCEGLDLDISECSGD

KEKQLICLFRN

AAALHGIWKSKKSTESSSTMEDFSRALDAYNTFLKTASEWLASKTTEKPVLLLGLGVSA

MLLSCFICGTV

FLSLFKEVYHEPKDRVCSLSNLLNLEEVFIFALLLILVISMGSSSMVEEEHYIWHFMVSTF

HLLLLFKTA

KSFKISKGMNILRDFKFGSIFSLLISGRLLRGWHQGGVNWTYLPDISKWLVQGGSGYVK

WIQLISIILVI

GLGLYTLFRTGSNRKGVRILAFGFSTCGFLVLLHVGRYQDELSTGFGATVTVKVIYYLLSI

SSIGASLVL

PWSALNKDKSFLAEVGDCLYLIGSAYILCWCLLQQLLQQPINSGPILLLLLQILAILCLSSS

DFQVNEWV

EITALYYMGMAGHFALGNSNTLATIDVAGAFIGISSHSTILSGILMFMITYASPMLFLLSLV

MYIGANLR

NHSNSTISTHRETTKARVPLPCSALHQLNTLDRIYCGLTTHEKSPLCMERLLSQVSL

AT2g22540，SEQ No.11

＞GM48963278 MADS box protein matter

atggtgagggagaagattcagattaagaagatcgacaacgccacggcgaggcaggtcacgttctccaagcgtcgcaga

gggctcttcaagaaagctgaggagctttcggttatgtgtgatgctgatgttgccctcataatcttctcttccactgggaagctcttt

gagtactcaagctcaagcatgaaggaaatacttgaaaggcatcatttgcactcaaagaacctagcaaggatggaacaac

catctctcgagttgcagctagttgaaaacagcaattgctctagattgagcaaggaagtggctgaaaagagccatcaactaa

ggcagctgagaggagaggatcttcaaggcttaaacatagaagaattgcaacaattggagaggtctcttgaaactggattgg

gccgtgtaatagaaaagaagggtgagaagattatgagtgagatcactgatctccaaagaaaggggatgctattgatggaa

gagaacgagcgacttaaacgtcacgtggcgggcataattaatggccaaaggcatggtggtgccgaatctgagaactttgtt

atggatgaaggtcagtcttcagagtctgttacgtacgtttgcaattccactggacccccccaagactttgaaagctcagatactt

ccctcaaattggggctaccatactccggctga

＞GM48963278 MADS box protein matter

mvrekiqikkidnatarqvtfskrrrglfkkaeelsvmcdadvaliifsstgklfeyssssmkellerhhlhsknlarmeqpslel

qlvensncsrlskevaekshqlrqlrgedlqglnieelqqlersletglgrviekkgekimseitdlqrkgmllmeenerlkrhva

giingqrhggaesenfvmdegqssesvtyvcnstgppqdfessdtslklglpysg*

＞KO08273(gi|20197885：c13869-13688，c12780-12702，c12415-12351，c12235-12136，

C12050-12009, c11897 11733) intend offering mustard karyomit(e) 2 clone F14M13 figure

Mi238, complete sequence

ATGGCGAGAGAAAAGATTCAGATCAGGAAGATCGACAACGCAACGGCGAGACAAGTGACGT

TTTCGAAAC

GAAGAAGAGGGCTTTTCAAGAAAGCTGAAGAACTCTCCGTTCTCTGCGACGCCGATGTCGCT

CTCATCAT

CTTCTCTTCCACCGGAAAACTGTTCGAGTTCTGTAGCTCCAGCATGAAGGAAGTCCTAGAGA

GGCATAAC

TTGCAGTCAAAGAACTTGGAGAAGCTTGATCAGCCATCTCTTGAGTTACAGCTGGTTGAGAA

CAGTGATC

ACGCCCGAATGAGTAAAGAAATTGCGGACAAGAGCCACCGACTAAGGCAAATGAGAGGAGA

GGAACTTCA

AGGACTTGACATTGAAGAGCTTCAGCAGCTAGAGAAGGCCCTTGAAACTGGTTTGACGCGTG

TGATTGAA

ACAAAGAGTGACAAGATTATGAGTGAGATCAGCGAACTTCAGAAAAAGGGAATGCAATTGAT

GGATGAGA

ACAAGCGGTTGAGGCAGCAAGTATGTGTCTTACCCTCTCTGTTGATAACAAATCCCTTTCTTT

TGTCTAC

CATTAACGTACACACCCCTAAATTTAATCCCCAGTTGTCTTACAACACATATGTTTGATCATACT

GTGAGA

TAA

The MADS-box protein matter [Arabidopis thaliana] that＞KO08273 gi|4544457|gb|AAD22365.1| infers

MAREKKQIRKIDNATARQVTFSKRRRGLFKKAEELSVLCDADVALIIFSSTGKLFEFCSSS

MKEVLERHN

LQSKNLEKLDQPSLELQLVENSDHARMSKEIADKSHRLRQMRGEELQGLDIEELQQLEK

ALETGLTRVIE

TKSDKIMSEISELQKKGMQLMDENKRLRQQVCVLPSLLITNPFLLSTINVHTPKFNPQLS

TTHMFDHTVR

At3g62170，SEQ No.15

＞KO15746 (gi|6899914) Arabidopis thaliana DNA karyomit(e) 3, BAC clones T17J13

ATGGCAGTAGGAAAGGTTGTGGTATCTGTGGCATCACTCCTCCTAGTGGTGGGTGTTGCCAT

AGGAGTTA

TCACGTTCGTTAATAAAGGAGGCGGCGCCAATGGCGACAGTAATGGTCCCATAAACTCTCAT

CAGAAAGC

GGTTCAGACAATTTGCCAGTCAACCACAGACCAAGGTTCGTGCGCGAAAACACTCGACCCA

GTCAAAAGT

GATGATCCAAGCAAGCTTGTCAAAGCCTTCCTGATGGCTACTAAAGACGCGATCACAAAATC

CTCAAACT

TCACGGCTTCGACCGAAGGAGGTATGGGTACTAACATGAACGCGACCAGCAAAGCCGTTCT

TGATTACTG

CAAGAGAGTATTGATGTACGCTCTTGAGGATCTTGAGACCATTGTTGAAGAGATGGGTGAGG

ATCTTCAG

CAGAGTGGAACTAAGCTTGACCAGCTCAAACAATGGTTAACTGGAGTTTTCAACTACCAAAC

CGATTGTC

TTGACGACATCGAGGAAGTTGAGCTAAAGAAGATCATGGGTGAAGGAATCTCTAACTCCAAG

GTTTTGAC

CAGTAACGCTATCGATATCTTCCATTCCGTTGTTACCGCGATGGCCCAAATGGGTGTCAAAG

TCGACGAT

ATGAAGAACATAACCATGGGAGCCGGAGCCGGTGGAGCTGCACGTCGCCTTCTTGAAGACA

ACGACTCTA

AGGGACTCCCCAAATGGTTTTCTGGTAAGACAGGAAGCTTATGGCTAAGGCCGGACGTGG

CGCTCCAGC

TGGCGGTGATGATGGTATCGGTGAAGGCGGTGGTGGTGGCGGTAAGATCAAGGCGACTCAT

GTAGTGGCT

AAGGATGGAAGTGGACAGTTTAAGACCATTTCTGAGGCGGTTATGGCTTGCCCGGATAAAAA

CCCTGGAA

GGTGCATTATCCATATCAAGGCTGGTATCTACAATGAACAAGTCAGAATCCCTAAGAAGAAGA

ACAACAT

TTTCATGTTCGGTGATGGTGCTACACAAACCATCATTACCTTTGACAGAAGTGTTAAACTTAG

CCCAGGA

ACCACTACTTCACTCAGTGGCACCGTTCAGGTCGAATCTGAGGGATTCATGGCGAAATGGAT

TGGGTTTA

AGAACACTGCTGGTCCATTGGGACACCAAGCGGTCGCACTCCGTGTGAACGGAGACCGTGC

GGTCATATT

CAACTGTAGATTCGACGGTTACCAAGACACACTCTACGTCAACAACGGACGTCAATTCTACA

GGAACATT

GTTGTATCCGGTACAGTGGATTTCATCTTCGGAAAATCCGCAACTGTGATCCAAAACTCTCTC

ATCCTCG

TCCGAAAGGGAAGCCCCGGACAATCCAACTACGTTACGGCTGACGGTAATGAAAAGGGTGC

AGCGATGAA

GATCGGTATCGTCCTCCATAACTGCCGTATCATACCGGACAAGGAGCTCGAAGCTGACAAGC

TAACCATC

AAATCGTACCTAGGAAGGCCGTGGAAGAAGTTTGCCACGACTGTGATTATTGGAACTGAGAT

TGGTGATT

TGATTAAACCAGAAGGATGGACCGAATGGCAAGGAGAACAAAACCACAAGACTGCAAATAC

ATTGAGTT

CAATAACCGTGGACCGGGAGCTGCCACTACTCAGAGGCCTCCTTGGGTTAAGGTGGCTAAG

TCTGCGGCT

GAGGTTGAAACTTACACCGTGGCTAACTGGGTTGGTCCAGCTAACTGGATCCAAGAAGCCAA

CGTGCCCG

TCCAACTAGGATTGTAA

＞KO15746 gi|6899927|emb|CAB71877.1|PECTINESTERASE-sample protein [Arabidopis thaliana]

MAVGKVVVSVASLLLVVGVAIGVITFVNKGGGANGDSNGPINSHQKAVQTICQSTTDQG

SCAKTLDPVKS

DDPSKLVKAFLMATKDAITKSSNFTASTEGGMGTNMNATSKAVLDYCKRVLMYALEDLE

TIVEEMGEDLQ

QSGTKLDQLKQWLTGVFNYQTDCLDDIEEVELKKIMGEGISNSKVLTSNAIDIFHSVVTA

MAQMGVKVDD

MKNITMGAGAGGAARRLLEDNDSKGLPKWFSGKDRKLMAKAGRGAPAGGDDGIGEG

GGGGGKIKATHVVA

KDGSGQFKTISEAVMACPDKNPGRCIIHIKAGIYNEQVRIPKKKNNIFMFGDGATQTIITF

DRSVKLSPG

TTTSLSGTVQVESEGFMAKWIGFKNTAGPLGHQAVALRVNGDRAVIFNCRFDGYQDTL

YVNNGRQFYRNI

VVSGTVDFIFGKSATVIQNSLILVRKGSPGQSNYVTADGNEKGAAMKIGIVLHNCRIIPDK

ELEADKLTI

KSYLGRPWKKFATTVIIGTEIGDLIKPEGWTEWQGEQNHKTAKYIEFNNRGPGAATTQR

PPWVKVAKSAA

EVETYTVANWVGPANWIQEANVPVQLGL

＞GM59639082 pectin methyl esterase sample protein

aacactagcaaagggaaggaaaagtaccttccccaataacccttctctccgatttggcttctctcatcggagggaaacaatc

aataaaagaaaaaaaaaaacccacaaaaagattataacaaaaaaagtatgaaaggaaaagtactaggttccgcagttt

ctctcatcctagtggtgggtgtggtaattggcgtggttgctgtagttcaaagcccaaagggcgtgaacaacaacaacaatgg

aggagaattgaaaaccagcaacagggccgtgacagcgttgtgccaaggctcagacgaccagaaactgtgccacgagg

ttctgtcatcttcgaacagcacagacccaaaggagtacatcgcaaccgtggtgagaacctcgatggacagcgtgatcaaa

gcgttcaacatgagcgacaggctgacggtggaacacggcaacagcagcgccggaatgaagatggctctggaggattgt

aaggacttgttgcagtccgccatccacgacctagaggcctcaggcgtgttggtcaaagagagcagcctacaggacgtgca

ccagcgcaccgccgagctcaagaactggttgggagccgttgttgcctaccagcaatcgtgcctggacggcttcgacaccg

acggcgagaagaaggtgcaggagcagctgcaatctgggagcttggacaacgttggaaaactcactggcttggcactcga

tgtcgtgtcagggatttcacacattctccaatccttggacttgaaccttgctctcaagcccgcctctcgccgccttctggaggtgg

accaggaaggctaccccacttgggtctccgctgctgatcgcaagctcttggctcagctcaacgacggtgcagtcttgcccca

tgctactgtggctaaggatggcagtggtcaatttaccactgttttggacgctattaactcctaccctaagaaacaccaaggca

gatacattatctacgttaaggctggtatctacgacgagtacatcaccgttgacaagaagaagcccaatcttttcatctacggtg

atggccccacaaataccatcatcactggccgcaagaactttcacgagggtaccaagacaatgagaaccgccaccttctct

actgttgccgaggatttcatggcgaagtcaattgcatttgagaacaccgctggtgcagaaggacaccaagcagtggcactt

cgcgtgcaaggtgatcgttcagtgttctttgattgtgctatgcgcggttaccaagacacattgtatgcccacgcccatcgtcagtt

ctaccgcaattgcgaaatctctggaacaattgatttcattttcggatactccaccaccttgatccaaaactcgaagatcttggtg

aggaagcccatggcaaatcagcagaacatagtggtggcagatggcacagggcagaagaacatgcccacaggaatagt

cctccataactgcgagatcatgccggaccctaccctcttggcagaccgcttgagcgtgaagacttacttggccaggccatgg

aaggcattctcaagggcagtgttcatcgagaatgttatcggggacttgattcagccagatggttatattccatggaacccaatt

gaaccaaacacacaggattgctacttcgctgagtttggcaacactggaccaggttccgttgctcaagcaagagccaagtttg

gcaaaggtctcattagcaagcaggaggctgcacaattcactgctgagccttggctccaagctagcacttggttgcctgctgct

ggcgttcctttcgatgctagcttcaccaaagcttaaggatccgagacaaaccagcatcttgagtgttataatctcttttttagccta

cctaaattaataaaactataaacaggaaatcgattacctaatttcaatgcagtcacacatagccactccattgtctgaggagc

tgatttgtaatcattcaataagcttttcatatttatgatttatgcctttttgtatcaaccacttcatctctgtcttcagtatgcatataacat

agactgatgatgggagaagcaatgggaattttgcttttggctatttaatggaaactacttaatttgccaaactaaaaaaaaaa

aaaaaaaa

＞GM59639082 pectin methyl esterase sample protein

mkgkvlgsavslilvvgvvigvvavvqspkgvnnnnnggelktsnravtalcqgsddqklchevlsssnstdpkeyatvvrt

smdsvikafnmsdrltvehgnssagmkmaledckdllqsaihdleasgvlvkesslqdvhqrtaelknwlgavvayqqsc

ldgfdtdgekkvqeqlqsgsldnvgkltglaldvvsgishilqsldlnlalkpasrrllevdqegyptwvsaadrkllaqlndgavl

phatvakdgsgqfttvldainsypkkhqgryyvkagiydeyitvdkkkpnlfiygdgptntiitgrknfhegtktmrtatfstva

edfmaksiafentagaeghqavalrvqgdrsvffdcamrgyqdtlyahahrqfyrnceisgtidfifgysttliqnskilvrkpm

anqqnivvadgtgqknmptgivlhncelmpdptlladrlsvktylarpwkafsravfienvlgdllqpdgyipwnpiepntqd

cyfaefgntgpgsvaqarakfgkgliskkqeaaqftaepwlqastwlpaagvpfdasftka*

At3g62180，SEQ No.17

＞KO15746 gi|6899914 Arabidopis thaliana DNA karyomit(e) 3, BAC clones T17J13

ATGAACACTCCAATCAAACTCTTCTTTCTCTTCATATTCGTTGCCGCAACCGCAACGGCTAAA

CCAGTCA

AACGAACGAACTACCTTTTCACACCGCACGCCAAAGCAGTCGCGGGAATCTGCACCGTTATC

CCAACGGA

CACAAGTCTATGTTGCAAAACCCTTAAACACGTTCCTACCAATGACCCCATCGAACTAATCCG

AGCATTA

GTGGCTGCCGCTGAAACATCCGTCAAACAAAGTGTGACTTTCCTCTCTGGAATCAAACCAAA

ACACATGT

CAGACGCAACCGCAACCGCAGTGGTCAACAGCTGCGAGAAAAACTTGAATTACGCGTTGGA

AGATTTCGC

CGATTTTTGGAAAGCTACGGGGAAAGATGTAACGACGTTGGCTCATAATTATTTTACGTGTAA

AAAAGAG

TTAATGTCAATCATGGGGTATCATTCGACTTGTTTGGACGATATTGAAGACAAGATTTTGTTG

AAGGAAG

TGGGGATTGGGATTGGAGTTGGGAAGAATCTAACAAGTGATTCTTTTGATGTGTTTAATAACT

TGAATAC

TATTTTCAAGACTTTTGGTATTAAGGTGAAGCTTAACGAGGAAGACACTTCGCCCCGACCGC

CACCCTTG

TCTGATTATTATTACTGA

＞KO15746N gi|6899928|emb|CAB71878.1| infers protein [Arabidopis thaliana]

MNTPIKLFFLFIFVAATATAKPVKRTNYLFTPHAKAVAGICTVIPTDTSLCCKTLKHVPTND

PIELIRAL

VAAAETSVKQSVTFLSGIKPKHMSDATATAVVNSCEKNLNYALEDFADFWKATGKDVTT

LHNYFTCKKE

LMSIMGYHSTCLDDIEDKILLKEVGIGIGVGKNLTSDSFDVFNNLNTIFKTFGIKVKLNEED

TSPRPPPL

SDYYY

The Rohapect MPE that＞BN43561885 infers

atgaacactccagtcatacatatattattgtttctcttcgtagctgccaccgcaacggcagccgctacac-accaaacgcgc

aaactaccttttcacacggcacgcaaaagcagtcgcgggaatatgcactgtaatccaaacaaacacaagtctatgctgca

aaacccttaaacacgttcctaccaatgatcccattgaattgatccgagcgttagcggttgcggctgaatcttccgtgaaaaata

gtgtggcttttctctcagaaatcaaaccaaaacatacatcaaacgcaaccgcagctgcagcgatcatcagctgcgggaaa

aacttgaaatacgcattggaagatttcaccgagttttggaaagctacggggaaagatgtaaagacgttggctcataactattt

cacgtgtaagaagacgctaatgtcgattatggggtaccattggacttgttttgatgatattgaagataagagtttgttgaaggaa

atggagattgggattggagttgggaagaatctaagcagtgattcgtatgatgtgttcaatggtttaaatactatttttaagacttttg

gtattaaggtgaagctgaacgaggaagacacttcgccccgaccgccaccattgtcggcttattactactga

The Rohapect MPE that＞BN43561885 infers

mntpvihillflfvaatataaatptkranylftrhakavagictviqtntslccktlkhvptndpieliralavaaessvknsvaflseik

pkhtsnataaaaiiscgknlkyaledftefwkatgkdvktlahnyftckktlmsimgyhwtcfddiedksllkemeigigvgkn

lssdsydvfngintlfktfgikvklneedtsprppplsayyy*

The Rohapect MPE that＞BN43580660 infers

atgaacattgcaatcaaactagcctttctcttcttaaccgtcacctcaaccgtaaccgctacaccagccaaacgcccagact

acctttccacacagcatgcaaaaacaatcgcgggaatatgcaccgttatccaaacagacacaagtctatgcaccaaaac

ccttaaagaagttcctagcgatgaccccatcacattaatccgagcattacctactgcggctgaatcatccgttaaaaaaagc

gtgactttcctcattggaatcaaaccaaaacatacatcaaacgcagtcgcaactgcagcgatcaacagctgcgagaaaaa

cttgaataacgcgttagaagatttcgctgagttttggaaagctacggggaaagatgtaacgacgttggctcataactatttcac

gtgtaagaaaacgttaacgtcgatcatggggtatcattcgacttgtttggatgatattaaagataagaatctgttgaaggaagt

ggagagtgggattggagttgggaagaatctaagcagtaactcgtttgatgtttttaatggtttgcatactattttcaatacttttgct

attaatgtgaagcttaacgaggaagacacttcgccccgaccgccaccattgtccgattattattactga

The Rohapect MPE that＞BN43580660 infers

mniaiklaflfltvstvtatpakrpdylstqhaktiagictviqtdtslctktlkevpsddpitliralptaaessvkksvtfligikpkhts

navataainsceknlnnaledfaefwkatgkdvttlahnyftckktltsimgyhstclddikdknllkevesgigvgknlssnsf

dvfnglhtifntfainvklneedtsprppplsdyyy*

At1g10120，SEQ. ID No.19

＞KO17997 arabidopsis thaliana chromosome | BAC T27|1 genome sequence, sufficient sequence

ATGGGTGGTGAGAGTAATGAAGGAGGAGAGATGGGCTTTAAGCATGGAGATGATGAGAGTG

GTGGGATCT

CTAGAGTTGGAATTACATCAATGCCCTTGTATGCAAAGGCAGATCCTTTCTTCTCTTCTGCAG

ATTGGGA

TCCAGTTGTCAATGCTGCTGCTGCTGGCTTCTCCAGCTCTCATTACCATCCTTCCATGGCGAT

GGATAAT

CCAGGGATGAGTTGCTTCTCTCATTACCAACCCGGTTCTGTTTCCGGTTTTGCAGCAGACAT

GCCTGCTA

GTCTTCTTCCGTTTGGTGATTGTGGTGGTGGTCAAATTGGTCATTTTCTTGGTTCAGACRRGA

AAGGGGA

AAGATTGATCAGAGCTGGAGAATCATCTCATGAGGATCATCATCAGGTTTCAGATGATGCTGT

TCTTGGT

GCTTCCCCAGTTGGGAAAAGAAGGCTACCTGAAGCCGAATCACAATGGAACAAGAAAGCTGT

GGAGGAAT

TTCAAGAAGACCCTCAAAGGGGAAATGATCAGAGCCAGAAGAAGCATAAAAATGATCAGAGT

AAAGAGAC

GGTGAACAAGGAGAGCTCACAAAGTGAAGAAGCACCGAAAGAAAACTACATTCATATGAGGG

CAAGAAGA

GGTCAAGCCACTAATAGTCACAGTCTTGCAGAGCGGATCACCGGAAAAGCGGTTATGCTCG

ATGAAATAA

TCAACTATGTTCAGTCATTGCAACAGCAAGTTGAGTTTTTGTCTATGAAACTTGCGACGGTGA

ATCCAGA

GATCAATATTGATATAGACAGGATTCTCGCCAAAGATCTTCTGCAGTCAAGAGACAGAAACAC

TCCTACA

CTCGGGCTGAATCCTTTTGCCGGTTTTCAAGGGAACATACCAAACCTTTTCTGCCACCACAAAT

CCACAAT

ACAACCCACTACCTCAGACAAACACTAGAGAGTGAACTACAAAACCTTTACCAGATGGGATTC

GTCTCAAA

TCCATCGACTATGTCCAGTTTCTCACCTAATGGTAACTAA

The protein [Arabidopis thaliana] of＞KO17997 gi|3540186|gb|AAC34336.1|AAC34336 supposition

MGGESNEGGEMGFKHGDDESGGISRVGITSMPLYAKADPFFSSADWDPVVNAAAAGF

SSSHYHPSMAMDN

PGMSCFSHYQPGSVSGFAADMPASLLPFGDCGGGQIGHFLGSDKKGERLIRAGESSH

EDHHQVSDDAVLG

ASPVGKRRLPEAESQWNKKAVEEFQEDPQRGNDQSQKKHKNDQSKETVNKESSQSE

EAPKENYIHMRARR

GQATNSHSLAERITGKAVMLDEIINYVQSLQQQVEFLSMKLATVNPEINIDIDRILAKDLLQ

SRDRNTPT

LGLNPFAGFQGNIPNLSATTNPQYNPLPQTTLESELQNLYQMGFVSNPSTMSSFSPNG

N

The bHLH transcription factor that＞GM59590559 infers

atgttgcattgtctcaacacttcctgttcggacatcacagtcttggaaagacaacgggaagcaaccatcaagtgccagaacc

accaaccaccctacttaacggactttaacgccgtcttcccttcttcttcttcttcttcttcttcttcctcttcttcttctcatagtcagggct

tgctcatgatgtgctcggattcctcgctcggccaggtcctaactcactccgtcaaacccgatccgggcgtctggcccgaattc

ggttttctacccgccatttcgagaacttgtagcagagacggagacttggtttcgcccaaggaaaacatggccagtgggaaa

gaaaatgctaagaagagaaagcctcagaactccaaggttgttgcggagattgataataacaaggacaaggacaagaga

gtcaaagtaactggcgaggaaggagaatccaaagtaaccgagcaccataccagaaacaagaatgccaaatccaacg

caaataagaacaacagagaaacctctgcggacacttccaaaggctccgaggttcaaaatcagaaaccagactacattca

tgtccgagcgcgtcgtggacaagccacggatagtcatagcttagctgaaagagtaaggagggagaagattagcgagag

aatgaagtatttgcaagatttaataccgggttgcaacaaagttgcagggaaagctggaatgcttgatgaaattattaactatgt

tcagtctcttcaacgccaagttgagttcttgtcaatgaaattagcggctgtaaacccaaggcttgacttcaacattgacgaact

gtttgccaaagaggtgtttccttcttgtgctcaaagttttccaaacatagggattccctcagatatgagtattagtaacaacccttc

gtatcttcagttcaattcagcgcagcaacttgtatcgtgctgtggtggattaataaacagcatgggaataagccctcccaacat

gggactccgaacgaacattattagtactagcactgtacctttgcccgaaacttttcttgactcttcctgtttcgctcaaattctaccc

tcctcaaattgggaaggtggtgatttccaaagcctttacaacgttgcctttgatcaagggcgaacagcatcttttccccctcagc

catttacaggtctagttgaagctagcaatctaaaaatggagatgtaa

The bHLH transcription factor that＞GM59590559 infers

mlhclntscsditvterqreatikcqnhqppyltdfnavfpssssssssssssshsqgillmmcsdsslgqvlthsvkpdpgv

wpefgffpaisrtcsrdgdlvspkenmasgkenakkrkpqnskvvaeidnnkdkdkrvkvtgeegeskvtehhtrnknak

snanknnretsadtskgsevqnqkpdyihvrarrgqatdshslaervrrekisermkylqdlipgcnkvagkagmldeiiny

vqslqrqveflsmklaavnprldfnidelfakevfpscaqsfpniglpsdmsisnnpsylqfnsaqqlvsccgglinsmglsp

pnmglrtniiststvplpetfldsscfaqilpssnweggdfqslynvafdqgrtasfppqpftglveasnlkmem*

At1g10130，SEQ.ID No.21

＞KO17997 arabidopsis thaliana chromosome | BAC T27|1 genome sequence, sufficient sequence

ATGGAAGACGCCTACGCCAGATCTGTCTCAGAGGTGCTTGATTTCTTTGGGGTAGACCCAAC

AAAGGGTC

TTTCTGATTCTCAGGTTGTTCATCATTCCAGGCTTTATGGCAGGAATGTACTGCCTGAAGAGA

AAAGAAC

GCCATTCTGGAAACTGGTTCTGAAACAGT TTGATGATTTACTTGTCAAGATATTGATTGTGGC

TGCAATT

GTTTCTTTCGTATTGGCTTTGGCTAATGGAGAGACTGGTTTAACAGCATTTCTGGAGCCTTTT

GTCATTC

TGCTGATATTGGCTGCAAATGCGGCAGTGGGGGTGATCACGGAGACTAATGCTGAGAAGGC

TCTTGAGGA

GCTACGTGCCTACCAAGCAAATATAGCTACAGTGTTGCGAAATGGGTGCTTCTCTATCCTAC

CAGCAACA

GAGCTGGTTCCAGGCGACATTGTTGAAGTTACTGTGGGATGTAAGATTCCAGCTGACCTGAG

GATGATTG

AGATGTCTAGCAATACGTTTCGAGTTGATCAAGCCATTCTAACTGGTGAAAGCTGTTCCGTG

GAAAAAGA

TGTTGACTGTACTTTAACAACAAATGCTGTCTACCAAGACAAGAAAAATATTTTATTTTCGGGA

ACTGAT

GTGGTCGCGGGTAGGGGAAGGGCTGTTGTCATTGGAGTTGGTTCAAACACCGCAATGGGTA

GCATACACG

ATTCTATGTTGCAGACAGATGATGAGGCAACTCCATTGAAAAAGAAGCTGGACGAGTTTGGC

AGCTTTTT

GGCTAAGGTAATTGCGGGTATTTGTGTACTTGTGTGGGTTGTCAACATTGGTCACTTCAGTG

ACCCTTCT

CATGGTGGATTTTTTAAAGGCGCAATTCACTATTTTAAGATTGCAGTTGCCCTTGCTGTTGCA

GCTATTC

CTGAAGGACTTCCTGCTGTCGTGACAACGTGTTTAGCTCTTGGAACAAAGAAAATGGCTCGT

TTGAATGC

TATTGTACGGTCATTACCATCTGTCGAGACGCTTGGGTGCACTACTGTAATTTGCAGTGACAA

GACTGGA

ACATTGACAACCAATATGATGTCGGTGTCTAAGATATGTGTAGTCCAATCTGCAGAGCATGGT

CCTATGA

TTAATGAATTCACTGTTAGTGGGACAACTTATGCACCAGAAGGTACCGTCTTTGACAGCAATG

GGATGCA

GCTTGACTTACCTGCTCAGTCACCTTGCCTTCATCATTTAGCAATGTGTTCATCACTCTGCAA

TGACTCC

ATCTTGCAATACAATCCAGATAAGGATTCTTATGAAAAAATTGGAGAGTCAACTGAAGTTGCT

CTTCGAG

TTCTTGCAGAAAAGGTTGGGCTCCCTGGTTTTGATTCAATGCCTTCTGCTCTAAACATGTTGA

GCAAGCA

TGAACGTGCATCATATTGCAACCATTATTGGGAAAACCAATTCAAAAAGGTTTATGTTTTGGA

GTTTACT

CGTGACCGAAAAATGATGAGCGTCCTATGTAGCCATAAGCAAATGGATGTTATGTTCTCAAA

GGGTGCTC

CAGAGAGTATAATAGCTAGGTGTAATAAAATTCTCTGCAACGGTGATGGTTCTGTTGTTCCTC

TAACTGC

TGCTGGCCGTGCAGAGCTTGAGTCGAGGTTTTACAGTTTTGGCGATGAAACATTGAGATGCT

TAGCATTA

GCATTTAAGACCGTGCCCCACGGTCAACAAACTATTTCCTATGATAATGAGAACGACCTGAC

GTTTATTG

GGTTGGTGGGAATGCTTGATCCACCAAGAGAAGAAGTGAGAGATGCTATGCTTGCGTGTATG

ACTGCTGG

GATACGTGTTATAGTTGTTACTGGGGATAACAAGTCCACAGCAGAGTCACTATGTAGAAAAAT

AGGGGCT

TTTGACAATCTGGTAGACTTTTCTGGTATGTCCTACACCGCTTCTGAATTTGAACGGCTTCCA

GCAGTGC

AGCAAACTCTAGCATTGCGACGGATGACACTTTTTTCCAGGGTTGAACCTTCCCACAAAAGG

ATGCTTGT

TGAAGCCCTACAGAAACAAAACGAAGTGGTGGCAATGACTGGTGATGGCGTTAATGATGCCC

CTGCATTG

AAGAAAGCTGACATTGGGATTGCCATGGGTTCTGGAACAGCTGTAGCAAAGAGTGCTTCAGA

TATGGTTT

TGGCTGATGATAATTTTGCTTCAATAGTTGCGGCTGTTGCAGAAGGAAGGGCTATATATAATA

ACACAAA

GCAATTCATTAGATACATGATTTCTTCAAATATAGGGGAAGTGGTCTGTATATTTGTTGCAGCT

GTACTG

GGAATCCCTGATACCTTGGCACCTGTTCAACTTCTGTGGGTCAATTTGGTAACAGATGGATT

GCCTGCCA

CTGCCATTGGCTTTAATAAACAAGATTCCGATGTTATGAAGGCAAAACCCCGAAAGGTTGGT

GAAGCAGT

GGTCACTGGGTGGTTATTCTTCCGCTATTTGGTTATCGGAGTTTATGTCGGCCTGGCCACTG

TTGCTGGC

TTTATATGGTGGTTTGTTTACTCTGATGGTGGTCCTAAACTTACTTACAGTGAACTGATGAACT

TTGAAA

CTTGCGCACTTAGAGAGACAACTTATCCCTGCAGCATATTTGAGGATCGGCACCCATCTACT

GTGGCTAT

GACAGTACTTGTTGTTGTCGAGATGTTTAATGCTCTAAATAACCTCAGCGAAAATCAATCCCT

TCTGGTT

ATAACCCCAAGGAGTAACTTATGGCTTGTTGGTTCAATTATCCTGACGATGCTTCTGCACGTG

CTAATAT

TATATGTTCATCCACTGGCAGTCTTATTTCTGTCACGCCATTATCCTGGGCCGAGTGGACTG

CTGTTCT

GTATCTTTCGTTTCCAGTTATCATCATCGATGAGCTTCTGAAGTTCCTCTCTAGAAATACAGG

CATGAGA

TTCAGGTTCAGATTGAGGAAGGCTGATTTACTCCCCAAGGACCGGCGTGACAAGTAG

＞KO17997 gi|13162529|gblAAC34328.2|AAC34328 calcium transport ATP enzyme, the ECA3[Arabidopis thaliana]

MEDAYARSVSEVLDFFGVDPTKGLSDSQVVHHSRLYGRNVLPEEKRTPFWKLVLKQFD

DLLVLILIVAAI

VSFVLALANGETGLTAFLEPFVILLILAANAAVGVITETNAEKALEELRAYQANIATVLRNG

CFSILPAT

ELVPGDIVEVTVGCKIPADLRMIEMSSNTFRVDQAILTGESCSVEKDVDCTLTTNAVYQD

KKNILFSGTD

VVAGRGRAVVIGVGSNTAMGSHDSMLQTDDEATPLKKKLDEFGSFLAKVIAGICVLVW

VVNIGHFSDPS

HGGFFKGAIHYFKIAVALAVAAIPEGLPAVVTTCLALGTKKMARLNAIVRSLPSVETLGCT

TVICSDKTG

TLTTNMMSVSKICVVQSAEHGPMINEFTVSGTTYAPEGTVFDSNGMQLDLPAQSPCLH

HLAMCSSLCNDS

ILQYNPDKDSYEKIGESTEVALRVLAEKVGLPGFDSMPSALNMLSKHERASYCNHYWE

NQFKKVYVLEFT

RDRKMMSVLCSHKQMDVMFSKGAPESIIARCNKILCNGDGSVVPLTAAGRAELESRFY

SFGDETLRCLAL

AFKTVPHGQQTISYDNENDLTFIGLVGMLDPPREEVRDAMLACMTAGIRVIVVTGDNKS

TAESLCRKIGA

FDNLVDFSGMSYTASEFERLPAVQQTLALRRMTLFSRVEPSHKRMLVEALQKQNEVVA

MTGDGVNDAPAL

KKADIGIAMGSGTAVAKSASDMVLADDNFASIVAAVAEGRAIYNNTKQFIRYMISSNIGEV

VCIFVAAVL

GIPDTLAPVQLLWVNLVTDGLPATAIGFNKQDSDVMKAKPRKVGEAVVTGWLFFRYLVI

GVYVGLATVAG

FIWWFVYSDGGPKLTYSELMNFETCALRETTYPCSIFEDRHPSTVAMTVLVVVEMFNAL

NNLSENQSLLV

ITPRSNLWLVGSIILTMLLHVLILYVHPLAVLFSVTPLSWAEWTAVLYLSFPVIIIDELLKFLS

RNTGMR

FRFRLRKADLLPKDRRDK

At1g07710，SEQ.ID No.23

＞GM59577994 ankyrin repetitive proteins matter family

atggtaggagattttcaagtgactatggagaaacagagcagttttcgggcatctacaatggaaaaacagaagagttttcgtg

gatttatggaaaaacagaaaagttttcgcattgttatggagaagcagctcagcttcatgggaagtgaaaggaagaagaac

aaggaatcacctgggaaacgtggtgacttaccaattcatttagcagctcgggcagggaacttgagtagagtgaaagagat

aattcaaaactattctaataatgagacaaaagatttgttggcaaagcagaacctagagggggagacccctctttatgtcgctt

cagagaatgggcatgctttggttgttagtgagatacttaactacttggacctgcaaactgcttctattgcagccagaaatggcta

tgatccattccatattgctgcaaagcagggtcatcttgaggtgctgagagaactactgcactcctttcccaacttggccatgac

cacagatttgtccaactcaactgctttacacacagctgcaactcaaggtcatattgatgtg aagctccttctggaatcagatt

ctaaccttgctaaaatagccaggaataatggtaaaactgtccttcactctgcggctagaatggggcatttggaagttgtgaaa

gccttactaaacaaggatccaagcactggatttaggactgataagaaaggtcaaactgccctacacatggctgtgaaagg

gcaaaatgaagaaattttgctggaattggtaaaacctgacccagcagttttgagtctggaagataataaaggaaatacagc

attgcatattgccacaaagaagggccgtactcagaatgttcgctgcttgttatcaatggagtgtatcaacatcaatgctacaaa

caaggctggagagactcctcttgatgttgcagaaaaatttggaagtccagaactcgtctccatattgagggatgctggggctg

ccaattctactgaccaaaggaaacctccaaatccatcaaagcaactcaagcagactgtcagtgacataaagcatgacgta

caatcccaactccaacagacacgtcagactggcatgagggtccagaaaattgcaaagaagctaaaaaagctccacatta

gtggcctgaacaatgcgataaactctgctactgttgttgccgttcttattgctacagttgcttttgcagccaccttcacagtccctg

gtcaatacgttgaagacaaaacacatggattttcacttggacaagcaaatatagcaaacaatgcagctttcctaatattttttgt

gtttgacagcctggcattgttcatctctctggcagttgtggtggttcaaacctctgtcgttgtgattgagcaaaaggcaaagaag

cagctcgtttttgtcattaacaagctcatgtggatggcttgccttttcatttccattgccttcatttctcttacatacgtggtggtgggat

cacactccagatggcttgcaatatatgctactgtgattggaagcttgataatgctctctacaattggctccatgtgctattgtgtaa

ttttgcataggatggaggagacaaaattgagggccgagagtcgatcgttctctatgtctcatgcatcagaccaagagatttta

aacagtgaatacaagagaatgtacgcactgtag

＞GM59577994 ankyrin repetitive proteins matter family

mvgdfqvtmekqssfrastmekqksfrgfmekqksfrivmekqlsfmgserkknkespgkrgdlpihlaaragnlsrvkei

iqnysnnetkdllakqnlegetplyvasenghalvvseilnyldlqtasiaarngydpfhiaakqghlevlrellhsfpnlamttdl

snstalhtaatqghldvvklllesdsnlakiarnngktvlhsaarmghlevvkallnkdpstgfrtdkkgqtalhmavkgqnee

illelvkpdpavlslednkgntalhiatkkgrtqnvrcllsmecininatnkagetpldvaekfgspelvsilrdagaanstdqrkp

pnpskqlkqtvsdikhdvqsqlqqtrqtgmrvqkiakklkklhisglnnainsatvvavliatvafaatftvpgqyvedkthgfsl

gqaniannaafliffvfdslalfislavvvvvqtsvvvieqkakkqlvfvinklmwmaclfisiafisltyvvvgshsrwlalyatvigsl

imlstigsmcycvilhrmeetklraesrsfsmshasdqeilnseykrmyal

＞KO18461 (gi|6579252) arabidopsis thaliana chromosome 1BAC F24B9 sequence, sufficient sequence

ATGGAAGGGGAAGAAGACACTGTGGCGGGTTCTAGCATACCAAAGAAGAAAATGATGAAAC

AGCTGACAG

GAAAACGCGACGACACTCTGCTTCATTCAGCAGTGAGACACGGAAACAAAGACAGAGTTGTT

GAGATTCT

TACGAAAACCAGAGAGTCTGAGTTGAATCAGCTGTTGGGGAAACAGAACCAGTCAGGCGAA

ACCGCACTC

TATGTTGCAGCAGAGTATGGTGATGTAGAGATTGTCAAGGAGATGATCAACTGCTATGATCTT

GCTCTCG

TTGAGATCAAAGCAAGGAACGGATTTGATGCTTTCCACATTGCTGCAAAGCAAGGAGATCTC

GATGTGTT

GAAGGTTTTAGCAGAGGCTCATTCGGAGTTAGCGATGACGGTGGATCTATCAAACACTACGG

CACTGCAC

ACAGCGGCAACACAAGGACACACTGAAGTGGTAAACTTTCTTTTGGAACTGGGAAGCAGCCT

TGCTGGAA

TTGCCAAGAGCAATGGTAAGACGGCCCTGCACTCTGCATCAAGGAACGGGCATGTCAAAGT

CATTAAGGC

TCTCTTGGCATCCGAACCTGCGATCGCAATAAGGATGGACAAGAAGGGCCAAACAGCCCTT

CACATGGCG

GTTAAAGGAACAAATGTTGAGGTCGTGGAGGAACTTATCAAAGCAGATAGGTCTTCTATCAAT

ATAGCCG

ACACAAAGGGAAACACAGCGTTGCACATTGCAGCCCGAAAAGGCAGATCTCAGATTGTCAAG

TTGCTATT

AGCCAACAACATGACAGACACAAAAGCTGTTAACCGATCAGGCGAAACCGCACTTGACACAG

CAGAGAAA

ATTGGAAATCCAGAAGTGGCTCTTATTTTACAGAAACATGGTGTTCCCAGCGCCAAGACCATT

AAGCCAT

CCGGGCCTAACCCCGCTCGGGAACTGAAACAAACCGTAAGCGATATCAAGCATGAGGTTCA

CAATCAGCT

TGAGCACACACGCCTGACCAGAAAACGTGTTCAAGGAATCGCCAAACAGCTTAACAAAATGC

ACACTGAA

GGTCTTAACAATGCAATCAACTCGACTACTGTTGTAGCTGTTCTTATTGCCACGGTCGCTTTT

GCAGCAA

TTTTCACTGTCCCGGGGCAGTATGTAGAAGACACAAGTAAAATTCCAGATGGGCATTCCCTC

GGGGAGGC

GAATATTGCATCGACGACTCCGTTCATAATTTTCTTCATCTTTGATTCGATCGCACTCTTCATC

TCCTTA

GCGGTCGTGGTGGTTCAGACATCAGTGGTGGTAATAGAGAGCAAGGCCAAGAAACAGATGA

TGGCTGTGA

TAAACAAACTCATGTGGCTTGCCTGTGTTCTCATCTCTGTTGCCTTTTTGGCTTTGTCGTTTGT

TGTTGT

TGGTGAAGAAGAGAAGTGGCTAGCCATTTGGGTGACTGCTATCGGGGCAACTATAATGATTA

CGACGTTA

GGGACGATGTGCTACTGGATAATACAGCACAAGATCGAAGCTGCCAATTTAAGAAACATTAG

AAGATCCT

CCATCAACAGTATATCTGGATCCTGGGGGATTCCCCAGCTTACGGATTCTGATATTCTCCAG

AACGAGTG

TAAGAAAATGTATGCAATCTGA

It contains AnK repetition PF|00023＞KO18461 gi|8439897|gb|AAF75083.1|AC007583_19

EST gb|Al996003 is from this gene.[Arabidopis thaliana]

MEGEEDTVAGSSIPKKKMMKQLTGKRDDTLLHSAVRHGNKDRVVEILTKTRESELNQLL

GKQNQSGETAL

YVAAEYGDVEIVKEMINCYDLALVEIKARNGFDAFHIAAKQGDLDVLKVLAEAHSELAMT

VDLSNTTALH

TAATQGHTEVVNFLLELGSSLAGIAKSNGKTALHSASRNGHVKVIKALLASEPAIAIRMDK

KGQTALHMA

VKGTNVEVVEELIKADRSSINIADTKGNTALHIAARKGRSQIVKLLLANNMTDTKAVNRSG

ETALDTAEK

IGNPEVALILQKHGVPSAKTIKPSGPNPARELKQTVSDIKHEVHNQLEHTRLTRKRVQGI

AKQLNKMHTE

GLNNAINSTTVVAVLIATVAFAAIFTVPGQYVEDTSKIPDGHSLGEANIASTTPFIIFFIFDSI

ALFISL

AVVVVQTSVVVIESKAKKQMMAVINKLMWLACVLISVAFLALSFVVVGEEEKWLAIWVTA

IGATIMITTL

GTMCYWIIQHKIEAANLRNIRRSSINSISGSWGIPQLTDSDILQNECKKMYAI

At1g07420，SEQ ID No.25

The C-4 sterol methyl oxidation enzyme that＞GM47133560 infers

atgctcccctacgcttccatcccggaggccgtggcggcgctgggccgcaacctcaccttcgcggagaccctctggttcaact

actccgccgccaagtccgattacttcctctactgccacaacattctgttcctcttcctcgtcttctccctcgtccccctccccctcgt

cttcctcgaattcaagcgcttctccttcgtctcttcccacaagatccaaccaaaagtccgcttgtccctggccgaaaccttcaag

tgctacaaagacgtcatgcgcatgttcttcctcgtcgtcggccccctccaactcatctcttacccttccatccagatgattgggat

caggacgggcttgccattaccttcgtggcgggagatcctctcgcagcttctggtgtactttctcgtagaggattacaccaattac

tggatccacaggtttctgcacaacgattgggggtacgagaagattcaccgcgtccaccacgagtaccatgcgcccattgga

ttcgccgcgccctatgcccactgggccgagatcttgatcctcgggattccctcctttcttgggcctgccatggttcctggccacat

tatcaccttctggctctggatagccttgcgccagattgaagccattgacacgcacagcgggtatgactttcctaggagtatcac

aaaatatatccattttatggtggtgctgagtatcatgattaccatcattacgttggaagacaaagccaaagcaattttgcttcag

ttttcacatactgtgattacatctatggaactgacaaggggtataggtatcagaaaaaaatacttcagaagttgaaggaaga

gttggcaaatggtgttgagcagaacggaggattatacaagactgactga

The C-4 sterol methyl oxidation enzyme that＞GM47133560 infers

mlpyasipeavaalgmltfaetlwfnysaaksdyflychnilflflvslvplplvflefkrfsfvsshkiqpkvrlslaetfkcykdv

mrmfflvvgplqlisypsiqmigirtglplpswreilsqllvyflvedytnywlhrflhndwgyekihrvhheyhapigfaapyah

waeililgipsflgpamvpghiitfwlwialrqiealdthsgydfprsitkypfyggaeyhdyhhyvgrqsqsnfasvftycdyiy

gtdkgyryqkkilqklkeelangveqngglyktd

The genome sequence BAC F22G5 of＞KO18461 (gi|7206858) Arabidopis thaliana is from karyomit(e) 1,

Sufficient sequence

ATGTGGTTGATGCAGTACCTTGTGACACATTTTAGCGACTTTCAACTGGCATGTATTGGGAGT

TTTCTCC

TCCATGAAAGCGTGTTTTTCTTATCTGGACTCCCTTTCATTTTTCTTGAAAGGCAAGGCTTTCT

CAGCAA

GTACAAAATTCAGACAAAAAATAACACACCTGCAGCCCAAGGAAAATGTATTACTCGCCTGTT

GCTTTAT

CATTTCTCCGTAAACTTGCCCCTGATGTTGGCCTCCTACCCTGTCTTCCGAGCCATGGGAAT

GCGAAGCA

GTTTTCCTCTGCCGTCCTGGAAAGAAGTGTCTGCCCAGATATATTCTACTTTATCATTGAGG

ATTTTGT

CTTCTATTGGGGTCATCGGATCTTGCATTCAAAATGGCTGTACAAGAACGTGCATAGTGTGC

ATCATGAA

TATGCCACACCATTTGGTTTGACATCAGAATATGCTCACCCCGCTGAGATTCTATTTCTGGGT

TTTGCTA

CCATAGTCGGTCCAGCTCTTACTGGCCCTCACCTAATTACTCTCTGGTTATGGATGGTGTTGA

GAGTGCT

GGAGACAGTTGAGGCACATTGTGGTTATCATTTCCCATGGAGCCTCTCAAATTTTCTTCCTCT

GTATGGA

GGTGCTGACTTCCATGACTACCATCACCGACTGCTATACACAAAGTCCGGAAACTACTCTTC

AACTTTTG

TGTATATGGACTGGATCTTTGGTACTGACAAGGGGTACAGAAGACTGAAGACCCTTAAAGAA

AACGGTGA

CATGAAACAAACGTGA

＞KO18461 gl|8778563|gb|AAF79571.1|AC022464_29 F22G5.23[Arabidopis thaliana]

MWLMQYLVTHFSDFQLAClGSFLLHESVFFLSGLPFIFLERQGFLSKYKIQTKNNTPAAQ

GKCTTRLLLY

HFSVNLPLMLASYPVFRAMGMRSSFPLPSWKEVSAQILFYFIIEDFVFYWGHRILHSKWL

YKNVHSVHHE

YATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLITLWLWMVLRVLETVEAHCGYHFPW

SLSNFLPLYG

GADFHDYHHRLLYTKSGNYSSTFVYMDWIFGTDKGYRRLKTLKENGDMKQT

The C-4 methyl oxidation enzyme that＞BN42488493 infers

atgaaagcgtcttcttcttatctggtctcccttttatttacctcgaaagacatggctttctcaccaagtacaaaattcaggcaaaaa

aacaacacacctgctgctcaaggaaaatgtatcactcgcctgttgctttatcatttctgcgtgaatttgcccctcatgatggcttc

ctatcctgtcttcaaagccatgggaatgcgaagcagttttcctctaccctcctggaaagaagtgtctgcccagatattgttctact

tcatcattgaggattttgttttctattggggacatcggatcttgcactcaaaatggctttacaagaacgtccacagtgtgcatcatg

aatatgccacaccgttcggtttgacatcagaatatgctcaccccgcagagattctattcctgggatttgctaccatagttggtcc

agctctcacaggcccccacctgattacgctctggttatggatggttctgagagtgcttgagacagtggaagcacattgtggct

atcatttcccatggagtctctcaaatttccttcctctgtatggaggtgctgacttccatgactaccatcaccgcctcctctacacaa

agtctggaaactactcttcacttttgtgtatatggactggatctttggtaccgataagggctacagaagactcaagtctcttaaa

gaaaatagcaacttgaaacaaacgtga

The sterol C-4 methyl oxidation enzyme that＞BN42488493 infers

mkasssylvsllftskdmafspstkfrqknntpaaqgkcitrlllyhfcvnl plmmasypvfkamgmrssfplpswkevsaqi

lfyfiiedfvfywghrilhskwlyknvhsvhheyatpfgltseyahpaeilflgfaivgpaltgphli-wlwmvlrvletveahcgy

hfpwslsnflplyggadfhdyhhrllytksgnysstfvymdwifgtdkgyrrlkslkensnlkqt*

The sterol 4-Alpha-Methyl oxydase that＞GM50246957 infers

atggcgtccctcatcgaatctggctggcagtacttgatcacacatttcagtgactttcaactggcgtgtttgggaagtttctttctac

atgaaggcgttttcttcttgtctggacttccctttatatggcttgagagggcagggtggatga caagg tacaaaattcaggcca

aaaataacacccctgcagctcaggagaaatgtattgttcgtctgttgctttaccattttggtgtcaatctacctgttatgattttttcat

atcctgtcttcacatacatgggcatgcggagtagtcttcccctaccgtcctggaaagtagttctaattcaaataatcttttacttcat

tttggaggactttatattctactggggacatagaatactgcacacaaagtggttatacaagcatgtgcacagtgttcatcatga

gtatgctacaccgtttggattgacttctgaatatgctcatcctgctgagatacttttccttgggtttgctaccatttttggtcctgccatt

actgggccccacttgataactctctggttatggatggttctgagagtcctagagacagttgaggctcattgtggttaccatttccc

atggagtctttccaacttccttccattgtatggaggagctgatttccatgactatcatcaccgtttattgtacaccaagtctgggaa

ctattcatcaacttttacttacatggaccggatatttgggactgatataggctacagaaagttgaaagcattgaagagcatagg

agttgaagacagtagcgagcaaaagaaacaataa

The sterol 4-Alpha-Methyl oxydase that＞GM50246957 infers

masliesgwqylithfsdfqlaclgsfflhegvfflsglpfiwleragwmskykiqaknntpaaqekcivrlllyhfgvnlpvmifs

ypvftymgmrsslplpswkwliqiifyfiledfifywghrilhtkwlykhvhsvhheyatpfgltseyahpaeilflgfatifgpaitg

phlitlwlwmvlrvletveahcgyhfpwslsnflplyggadfhdyhhrllytksgnysstftymdrifgtdigyrklkalksigveds

seqkkq*

The sterol 4-Alpha-Methyl oxydase that＞OS32661132 infers

atggcggcgtccgccctcgactccgcctgggagggcctcaccggcagcttcaccgagttccagctcgccaccgtcgtcac

cttcctcctccacgagaccgtcttcttcctctccggcctcccctccctcctcttcgagcgcttcggcctcttcgccaagtacaaga

tccagaagaagagcaataccccttcttaccagaatagatgtgtgctgcgtctcattctgtaccatgtctgtgtgaacttgcctgta

atggttttatcctaccctgccttcaaattcatgggcctgaggagctctcttcctctgccacactggacggttattgtttctcaagttct

tttttactttgtactcgaggattttatattttattggggacatagggcactgcacaccaaatggctatacaagcatgttcacagcgtt

caccatgaatatgctacaccctttggcttgacttcagaatatgcccaccctgctgaaattttgttccttgggttcgccacaattgtt

ggtccggccctcactggtccgcacttgttcactctatggctgtggatggtgttgagggtattggagacagttgaagctcacagt

ggataccatttcccatggagcccatcaaatttcttgccactgtatggaggctccgactttcatgactatcatcaccgtgtgctcta

caccaaatcaggaaactacgcctctacttttgtttacatggactggctgtttggcacggacaaggattaccgcaatgccaagg

ctatcgaggagaaagacgggaagcatttgtaa

The sterol 4-Alpha-Methyl oxydase that＞OS32661132 infers

maasaldsawegltgsftefqlatvvtfllhetvfflsglpsllferfglfakyklqkksntpsyqnrcvlrlilyhvcvnlpvmvlsyp

afkfmglrsslplphwtvivsqvlfyfvledfifywghralhtkwlykhvhsvhheyatpfgltseyahpaellflgfativgpaltg

phlftlwlwmvlrvletveahsgyhfpwspsnflplyggsdfhdyhhrvlytksgnyastfvymdwlfgtdkdymakaieek

dgkhl*

At2g26890，SEQ No.27

＞KO18598 (gi|20197284) arabidopsis thaliana chromosome 2 clone F12C20 figure B68, sufficient sequence

atggattccgtctctagaggtgccgttgcttcaacaaccggcggtgctgtggaagagccggagtatctagctaggtatcttgtt

gttaaacattcatggagaggtcgttataagaggatcctttgtatttcgagcggcggaattgttacgcttgatcctaatactcttgct

gttactaattcttatgatactggaagtaattttgatggtgcttcacctctggttggaagagatgagaacacggagagtgttggtg

gtgagtttactgtcaatgttagaacggatgggaaagggaaatttaaggctatgaagttctcttctaggtgcagagcgagtatttt

gaccgagttgtatcggcttagatggaatcaaattagacctgtggctgagtttcaggtgctacatcttaggagacggaacgca

gaatgggttccttataaattgaagatcacctttgtcggtctggagcttgtcgactcaaaatctggtaattcacgctggattttggat

ttcagagacatgggttccccagcaatcattcttctctctgatgcataccggacaaaatctgcggactctgctgggtttgttctgtgt

cccatgtatgggagaaagtcaaaagcttttagagctgcacccgggacaacaaattcctccattgtcgcaagtttggctaaga

ctgcaaagtccatggttggggtattcttgtcagtcgatgattcacaattgctgacagtatcagagtatatgacacgaagggcta

aagaagcagttggagctgaagaaactcctaatgggtggtggtctgttactagattaagatctgctgctcatggaactctgaac

atgcctggactaagcttagcaattggccccaaaggaggacttggtgagcatggggatgctgtagcccttcagcttattcttact

aaggcctcccttgttgagagacgaatagataactatgaagttgttatcgttcgtcctctatcttcagtaagttcacttgtccggttc

gctgaggaaccccaaatgtttgctatcgaattcagtgatggatgtccagttcttggacactgcccgataccagtattaccaag

gcttactatgcctggtcatcgcattgatccaccttgtggaagggttagtttgatctctggaccacaacatcttgttgctgatttgga

aacttgctccctacatctgaaacatttagctgctgctgcaaaagatgcagttgccgaaggtggttctgttcctggttgtagggct

agattatggcgcagaataagggagttcaatgcttgtatcccgtatacaggtgtgcccgctaatagtgaagtccctgaggtgac

tttgatggcattaattacaatgctaccatcaactccaaatctccctgtagacgcccctcctttgccacctccttcacccaaagca

gcagcaactgtcattggctttgttacatgtttgcgtaggttattgtcatccaggagtgcagcatcccatataatgtcattccctgct

gctgttaacaggataatgggtttacttaggaacggttctgaaggtgtagctgctgaagctgcggggcttattgcgtccctccata

ggcggttggtcagcagatctgagcactgcaccagattccagaggagaaaaacatgcaactatcatgcataccaagtctgtt

ttgtttgctcaacagggttatgttactattctggtcaatcgattgaaacccatgtcagtctcacctctgttttccatggcgattgttga

agtctttgaggctatggtttgtgatccacacggagagactacccaatacactgtttttgtagaattgttacgacagatagctgcc

ctacgacgtcgtttatttgcactctttgcacatcctgcagagagtgttagggaaaccattgctgttatcatgcgtacaatagctga

agaagatgcaattgctgcagagtcaatgcgtgatgctgctttgcgcgatggtgctttgttgagacatttattgaatgcattttccct

tcctgccagtgagcggcgcgaggtaagtaggcagcttgtggcactctgggcagattcttaccaaccagctttggatctactgt

ctcgagttctgcctcctgggcttgttgcatatttgcatacacgtcccgatgatgttgtcgatgatacagatcaagaaggttcttca

acaaataggcggcagaaaagattacttcagcagagaagaggtcgcatagctaagggaatgggtgctcaagatattcctct

tccccctggtaataatgttgaggctggcgatgcagcaaaacatatgagtgcaaatgctagtgtacccgataactttcaaagg

cgggcagcagattcttcctctgaagcttccaatcctcaggcttctgcttttccaggtgttgacagtactattgcaggggtttcaca

aaatggctatccagcatttgcttcagtcaccacaaatgcaaatgggcatgagcaacctgagactaatgcatccgatgtggtt

ggttctgacccaaacttgtatggcatccagaattcagtgcttccagcacctgctcaagttattgtagaaagtacagctgtagga

tccggaaagctacttctaaattggcgtgagttttggcgagcctttggccttgatcataatcgtgcagatctcatctggaatgagc

gtacaaggcaagaattaatagaagctttgaaggctgaagtccacaacctagatgtcgagaaagagcgcacagaagatat

ttcccctggtgatgtcgaggccacaactggccaggagattatcccacgtatatcttggaactattctgaattctctgtcagttatc

gtagcttatcaaaagaagtttgtgtgggccagtattacctacgcttattgcttgaaagtggcaacgctggcaaggcacaagat

ttccctctccgtgatccagttgcttttttcagggcactctatcatcgtttccagtgtgatgctgatatggggcttactattgatggtgct

gttccagatgaattgggttcatcaggcgactggtgtgatatgagtaggcttgatggttttggtggagggggaggagcttctgtta

gggagctttgtgcaagagcaatggcgattgtctatgagcaacactacaacacaataggtccttttgaaggcactgcacatatt

acagcactgattgataggacgaatgatagagctttgaggcatcgcctactacttctcctaaaggccctagttaaggtcttgtta

aacgtcgaaggttgtgttgtggttggtggttgtgtcctagctgtagatctgctgactgttgttcatgaaaactcggagaggactcc

tattccattacagtccaatttaattgctgctactgcatttatggaaccacctaaggaatggatgtacatagacaaaggtggtgca

gaagtgggacctgtagagaaggacgtcatcagaagtttatggtccaaaaaggatattgactggacgacaaagtgtcgggc

tttaggaatgtcagactggaagaaattgcgtgatatccgtgaacttagatgggcagtagctgttcgagttccagtcctcacacc

tagtcaggtaggggatgctgcattgtccatattacatagcatggtttcggcacattcagatttggatgacgctggagagattgta

actccaacaccaagagtaaaacgtatcttgtctagtacacgttgtcttcctcacattgctcaggctttgctatctggcgaaccag

ttattgtggaggctggtgctgctctcttgaaagacgttgttaccagaaactctaaggcaatgatccgactgtacagtacagggg

ccttttactttgcccttgcttaccctggatctaatctttactcaatcgcacaactcttctcggtcacccatgtccatcaagctttccatg

gtggggaagaagctactgtttcctcctctctgcccctggctaaacgaagcgtattgggtggtcttctcccagagtccttactatat

gtattagagcgcagtggaccagctgcgtttgcagctggcatggtttctgattccgatacgccggagattatatggacacataa

aatgcgagcagaaaatcttatatgtcaggttttgcagcatcttggtgattatcctcagaaattgtcacagcactgccattctctct

atgattatgctcccatgccacctgttacgtatccagaacttagagatgagatgtggtgtcaccgttattatctcagaaatttatgtg

atgagattcaatttcctaattggccgattgttgaacatgttgagttcttacaatcattacttgtgatgtggcgtgaagagttgactag

gaaacccatggatctttctgaaggagaagcttgcaaaattctagaaatatccctgaacaatgtatcaagtgatgacctaaac

cggactgcttcagttgagttgaatgaggaaatatctaatatatccaaacaaattcaaaaccttgatgaagagaaactaaagc

gccagtataggaagcttgcaatgaggtaccatcctgacaagaatccagaaggaagagaaaagttcctggctgttcaaaa

agcttatgaatgcctacaggcaacaatgcaaggattgcaaggtcctcagccgtggaggttgctgcttttactgaaagcgcag

tgcatcttatatcgccgttatggacatgtgttacgaccgttcaaatatgctggctatccgatgttacttgatgcagttacagtggac

aaggatgacaacaactttctatctaatgatagatcccctcttcttgttgcagcatctgagcttgtttcgttaacctgtgctgcctcgt

cattgaatggtgaagaattagtgagagatggtggtgtgcagcttctatcaactcttctttcccgctgcatgtgtgtggttcagcca

acaacttcacaacacgaaccagctgcgatcattgtcacaaatgtaatgcgtacactttcggtaataagtcagtttgagagtgc

gagggctggatttctagagttacccagtctgattgaagacattgtgcactgtacggaattagaacgtgtgcctgcagccgttga

tgctgctctccagtccattgccaaggtttctgtcttccccgaacttcagcatggtctgctaaaggctggtgccttatggtatattctc

ccattattactacagtatgactcaactgctgaggaatctaattctgtcgagtctcatggggttggagttagcattcaaattgccaa

gaatgagcatgccttacaagcatcacaagccctatcaaggcttactgggctgtgtgcagatgagagtttgacaccttacaat

gctactgcggctgatgttctcaaagcattactgacgccaaaacttgctagtttgttgaaagatgaagttgccaaggatttgttatc

caaactgaacacaaatttggagacaccagagattatctggaactctgcaactcgatcagagcttttaaattttgtggatgaac

aacgcgcctgccagtgccctgatggttcatatgatctgaaaaatgctcaatctttttcgtatgacgcactgtcaaaagaggtctt

tgttggcaatgtttacttgaaggtctataatgatcaacccgactcagagatcagtgaaccagaatcattctgcaatgccctaat

cgactttatatcatcattagtgcatactgagttgccctctgtttccgaggaccaaaatttgatcgaagacagaaactcatctaat

gatactccagagcttcaaagtagcgtcgcagaaccgtcgttgattgaagaacattccgatcatcagccatcatctgagggg

atgaagaacgaagaatgttttctgattgatcacctccaattaggattgactggctcttcagaacttgcttacaaagtatccagatct

ggcttcagtgttttcgtctaaggagagattgttacctctctttgaatgtttttctgtggccattgcatcaaaaavcagatattccaaaa

ctctgcctcaatgtcctctctcggttaacagcttatgctccttgcttggagacgatggtatctgatggatctagtcttcttctcctctta

caaatgcttcattctgcaccttcttttcgcgagggtgctctccatgttctttatgctttggcaagcacaccagaacttgcttgggctg

ctgcaaaacatgaagaaattcccttgcagcaaagagctgcagcggcttctttgttggggaagctcgtcgcacaaccaatgc

atgggcctagagttgctatcacacttgtgagattccttcctgacggtcttgtatctataattcgtgatggacctggggaggctgttg

tccatgcacttgagcggaccactgagactccagaacttgtgtggacaccagcaatggcagcatctttatccgcacagattgc

aaccatggcatcagatatttatcgtgaacaacagaagggttctgttattgaatgggatgtaccagagcagtcagctggtcaa

caagaaatgagagacgagccacaggttggtggaatctatgtcaggcgtttcttaaaagatccaaaatttcctctgagaaatc

caaaacgattcttggaaggactgctggatcagtatttgtcagcaatggccgcaacacattacgaacaacatcctgttgaccct

gagctccctctccttctctctgctgcattggtttctttgttgcgtgtgcatcctgcacttgcagatcacattggacatcttgggtatgtc

ccaaaacttgtcgctgctgtggcatatgaggggaggcgggaaacaatgcttctggcgaagtgaaggctgaagaaattgg

ctctgatggagtgaatgagtctatactgatccctcaagtctacctgggcaaacccctcaagaacgtgtgcgccttagttgtttacgt

gtgcttcatcaacttgcagctagtaccacatgtgctgaagcaatggctgcaactagtgctggaaatgcacaggtggttccact

tctcatgaaagcaataggatggcttggtggaagcattttagcactcgagacacttaagcgtgttgttgttgctggaaatcgggc

cagagatgcgcttgttgcgcagggtctaaaggttggtctcattgaggttcttcttgggctgcttgactggaggacggggggtag

gtatgggctcagttctcacatgaaatggaatgaatcggaagcatcaatcgggcgggtacttgcagttgaggttagtgttgaatt

tgttagcgagatgtttgttatgtgtgttacacatgtattgcatggttttgcaacagaaggagcacattgctcaaaagtgcgtgaga

tacttgacgcgtcagaagtgtggaggcatataaagaccaaaagcatgacttgttcctgccatcaaacacacaatcagcgg

caggggtggctggctttattgagaactcatccaacagtctcacttacgctcttaccgctcctcctccgccttcgcatccttga

＞KO18598 gi|3426038|gb|AAC32237.1| agnoprotein matter [Arabidopis thaliana]

MDSVSRGAVASTTGGAVEEPEYLARYLVVKHSWRGRYKRILCISSGGIVTLDPNTLAVT

NSYDTGSNFDG

ASPLVGRDENTESVGGEFTVNVRTDGKGKFKAMKFSSRCRASILTELYRLRWNQIRPV

AEFQVLHLRRRN

AEWVPYKLKITFVGLELVDSKSGNSRWILDFRDMGSPAIILLSDAYRTKSADSAGFVLCP

MYGRKSKAFR

AAPGTTNSSIVASLAKTAKSMVGVFLSVDDSQLLTVSEYMTRRAKEAVGAEETPNGWW

SVTRLRSAAHGT

LNMPGLSLAIGPKGGLGEHGDAVALQLILTKASLVERRIDNYEVVIVRPLSSVSSLVRFAE

EPQMFAIEF

SDGCPVLGHCPIPVLPRLTMPGHRIDPPCGRVSLISGPQHLVADLETCSLHLKHLAAAAK

DAVAEGGSVP

GCRARLWRRIREFNACIPYTGVPANSEVPEVTLMALITMLPSTPNLPVDAPPLPPPSPKA

AATVIGFVTC

LRRLLSSRSAASHIMSFPAAVNRIMGLLRNGSEGVAAEAAGLIASLIGGWSADLSTAPDS

RGEKHATIMH

TKSVLFAQQGYVTILVNRLKPMSVSPLFSMAIVEVFEAMVCDPHGETTQYTVFVELLRQI

AALRRRLFAL

FAHPAESVRETIAVIMRTIAEEDAIAAESMRDAALRDGALLRHLLNAFSLPASERREVSR

QLVALWADSY

QPALDLLSRVLPPGLVAYLHTRPDDVVDDTDQEGSSTNRRQKRLLQQRRGRIAKGMGA

QDIPLPPGNNVE

AGDAAKHMSANASVPDNFQRRAADSSSEASNPQASAFPGVDSTIAGVSQNGYPAFAS

VTTNANGHEQPET

NASDVVGSDPNLYGIQNSVLPAPAQVIVESTAVGSGKLLLNWREFWRAFGLDHNRADLI

WNERTRQELIE

ALKAEVHNLDVEKERTEDISPGDVFATTGQEIIPRISWNYSEFSVSYRSLSKEVCVGQYY

LRLLLESGNA

GKAQDFPLRDPVAFFRALYHRFQCDADMGLTIDGAVPDELGSSGDWCDMSRLDGFGG

GGGASVRELCARA

MAIVYEQHYNTIGPFEGTAHITALIDRTNDRALRHRLLLLLKALVKVLLNVEGCVVVGGCV

LAVDLLTVV

HENSERTPIPLQSNLIAATAFMEPPKEWMYIDKGGAEVGPVEKDVIRSLWSKKDIDWTT

KCRALGMSDWK

KLRDIRELRWAVAVRVPVLTPSQVGDAALSILHSMVSAHSDLDDAGEIVTPTPRVKRILS

STRCLPHIAQ

ALLSGEPVIVEAGAALLKDVVTRNSKAMIRLYSTGAFYFALAYPGSNLYSIAQLFSVTHVH

QAFHGGEEA

TVSSSLPLAKRSVLGGLLPESLLYVLERSGPAAFAAGMVSDSDTPEIIWTHKMRAENLIC

QVLQHLGDYP

QKLSQHCHSLYDYAPMPPVTYPELRDEMWCHRYYLRNLCDEIQFPNWPIVEHVEFLQS

LLVMWREELTRK

PMDLSEGEACKILEISLNNVSSDDLNRTASVELNEEISNISKQIQNLDEEKLKRQYRKLAM

RYHPDKNPE

GREKFLAVQKAYECLQATMQGLQGPQPWRLLLLLKAQCILYRRYGHVLRPFKYAGYPM

LLDAVTVDKDDN

NFLSNDRSPLLVAASELVSLTCAASSLNGEELVRDGGVQLLSTLLSRCMCVVQPTTSQH

EPAAIIVTNVM

RTLSVISQFESARAGFLELPSLIEDIVHCTELERVPAAVDAALQSIAKVSVFPELQHGLLK

AGALWYILP

LLLQYDSTAEESNSVESHGVGVSIQIAKNEHALQASQALSRLTGLCADESLTPYNATAA

DVLKALLTPKL

ASLLKDEVAKDLLSKLNTNLETPEIIWNSATRSELLNFVDEQRACQCPDGSYDLKNAQS

FSYDALSKEVF

VGNVYLKVYNDQPDSEISEPESFCNALIDFISSLVHTELPSVSEDQNLIEDRNSSNDTPEL

QSSVAEPSL

IEEHSDHQPSSEGMKNEECFLIDHLQLGLTALQNLLTKYPDLASVFSSKERLLPLFECFS

VAIASKTDIP

KLCLNVLSRLTAYAPCLETMVSDGSSLLLLLQMLHSAPSFREGALHVLYALASTPELAW

AAAKHEEIPLQ

QRAAAASLLGKLVAQPMHGPRVAITLVRFLPDGLVSIIRDGPGEAVVHALERTTETPELV

WTPAMAASLS

AQIATMASDIYREQQKGSVIEWDVPEQSAGQQEMRDEPQVGGIYVRRFLKDPKFPLRN

PKRFLEGLLDQY

LSAMAATHYEQHPVDPELPLLLSAALVSLLRVHPALADHIGHLGYVPKLVAAVAYEGRR

ETMSSGEVKAE

EIGSDGVNESTDPSSLPGQTPQERVRLSCLRVLHQLAASTTCAEAMAATSAGNAQVVP

LLMKAIGWLGGS

ILALETLKRVVVAGNFARDALVAQGLKVGLIEVLLGLLDWRTGGRYGLSSHMKWNESEA

SIGRVLAVEVS

VEFVSEMFVMCVTHVLHGFATEGAHCSKVREILDASEVWSAYKDQKHDLFLPSNTQSA

AGVAGFIENSSN

SLTYALTAAPPPPSHP

At2g35050，SEQ ID No.29

No. 2 karyomit(e) F19I3 clones of＞KO18598 (gi|20197115) Arabidopis thaliana ve016 map-area, complete sequence

atggatcagcaaaaggttatgaacatgttcggtatactgcccctgaccctagagatgagggacttggctccattaatcaaa

ggttttcccacgactcttcaactaatgttaacacttatgtacgacctccagattatggtgtttcaacccctgctcggccagtgcta

aactactcaatacagaccggtgaagaatttgcttttgagtttatgagagatagggttattatgaaaccgcagttcatcccaaat

gtgtatggtgagcacagtggtatgcctgtttctgttaacttaagtgctctgggaatggttcatccaatgtcagagagtggcccta

acgctacagtgcttaacatagaagaaaaacgtcagagctttgagcacgagaggaaacccccttctagaattgaagataag

acctatcatgaactggtccagtcagccccagttatctcttcgaaaaatgatactggtcaaaggcgtcatagtttggtttcttctag

agcttctgatagctctttgaaccgtgcgaagttcttgtgtagttttggtggtaaagttataccccgccccagagatcagaaactta

ggtatgtaggtggtgaaacgcgatcatacggattagcaagactatttctttccaagaactcatgcataaaatgaaagaaata

tttcctgaagcacgcaccataaaatatcagctgccaggagaggatcttgatgccctagtctctgtatcttctgacgaggatttac

aaaacatgatggaagaatgtatcgtgtttggtaatggaggatctgagaagcccaggatgttcttgttttcaagcagtgatatag

aggaggctcagtttgttatggaacatgcagagggtgattctgaggttcagtatgttgttgctgtcaatgggatggatctaagttc

acggagaagttcccttggattaagtcctcccgggaacaatttggatgaactacttcatgggaattttgataggaagatcgatc

gggctgtaccagaaccagcagtggcttcgcttactcccttagcaggtaatgaatctttaccagcgagccaaacttctcaacct

gtaacaggattttctactggaaatgagccattttcacagccttatctaggacaacaattgcagttccccggacttggtaaccac

caaatttacacgtcaggtcacatggcaagcataggctatatagatgagaagaggtctgctcctttacatgttcaaccacaac

ctcattatatcccgtattctgtgaatcctgaaacacctcttgaaagcctggtgccccactatccacaaaaacctgagcaagga

tttttgcgtgaggagcagatctttcatgtacaagatccagaaacttcatcaaaagaggccaaaatgagaagagatgactcat

ttcagaaggtaaatgatcatcctatatctactgtcgagagcaatctttcagcaaaggagccaaagatgaggagagaatcctc

aaccccaagggtcaatgagtatcctgtttcttctatgcctagtgatttaatagtcccagatgacctcccgaaggaagaagctcc

aattgtcacacaaacatctagttcaacaccagatccaagttcttcaactctctcagagaaaagtcttaggaaatccgaggac

catgttgagaacaatctgtcagcaaaggagccaaagatgagaaaagaacactccaccacaagggtcaatgaatattccg

tttcctctgtatctagtgattctatggtcccagatcaagccctcaaggaagaagctcctatttccatgaagatatccaattcaaca

ccagatccaaagtccttggtttatccagaaaaaagtcttagaacatcccaggagaaaacgggtgccttcgatacaacaaat

gaaggcatgaaaaagaatcaggacaatcaattttgtctgcttggaggattctcagtatctggacatggtacttcaaataatagt

tcatctaatgtgagcaatttcgaccagcctgtgactcagcaaagagtctttcattctgagcgaactgtacgagatccaacaga

aactaaccgtttgtctaaatctgatgattcccttgcttctcaatttgtaatggctcaaacaacatcagatgctttcctgcctatcagc

gaatcatctgaaacttcttcatgaagcaaatatggagtcccagaatgttcatcctactgcgccagtaataccagctcctgatag

catctggacagccgagggtagtatgtcacagtctgassaassasscgtggassctaacaccccggagcatgtaagtcag

acagagacyyvsgcaaaggctgttccacaaggacacaatgagaagggggatatagttgttgatataaatgataggtttcctc

gtgagtttcttgctgatatattaaaaacgaaagagtctctgaacttccctggattagggccattgcatgccgatggagctggtgt

gagtttaaatattcagaataatgaccctaaaacttggtcgtattttcgaaatttggcgcaggatgagtttgagaggaaggatct

atcccttatggatcaggaccaccctggatttcccacttccatgactaacaccaacggagttcctattgattatagctacccacc

attgcagtctgagaaagttgcctcaagtcagatacatccacaaatccactttgatggaaatatcaagccagatgtgtctacca

ttaccatacctgatttgaacacagtagacacacaagaagattacagtcagtcacaaatcaaaggtgctgaaagcacggat

gcaactctgaatgctggagttcctcttattgactttatggctgcggatagtggcatgaggtctctgcaggtcattaaaaatgacg

acttggaagaactgaaggaattaggttctggtacttttggaactgtttatcacggaaaatggaggggtacagatgttgctatca

agcgaataaaaaggagctgttttattggtcgttcatctgaacaagagagattgacctcggagttctggcatgaagcagaaatt

ctttcaaagcttcatcatccaaatgttatggcattttacggcgtagtgaaagatggaccaggaggaactttagctacagtgaca

gagtacatggtcaatggatcgctcaggcatgttctgctcagcaacaggcaccttgatcgacgtaagcgacttatcattgcaat

ggacgcagcttttgggatggaatatttgcactcaaagagcatagtgcatttcgatttgaagtgtgataacttgcttgtcaacttaa

aggatcccgcccgtcccatatgcaaggttggtgattttggtctgtcaaagataaaaagaaacactttggtcactggcggtgta

aggggaaccctcccttggatggctcccgagctacttagtggaagcagcagcaaagtttctgaaaaggttgatgtgttctctttc

ggaattgtcttatgggaaattcttaccggtgaggaaccctacgccaatatgcattatggggcaataatcggaggcatagtga

acaatacattgagaccaaccgtgccaaactactgtgacccggagtggagaatgctgatggagcagtgttgggctcctgac

ccatttgttcgacctgcgttcccggaaatagccagacgtctccgcaccatgtcctcctctgcggtccacacaaaaccacacg

ctgtcaaccaccaaatccacaagtaa

The protein kinase [Arabidopis thaliana] that＞KO18598 gi|3033400|gb|AAC12844.1| infers

MDQAKGYEHVRYTAPDPRDEGLGSINQRFSHDSSTNVNTYVRPPDYGVSTPARPVLN

YSIQTGEEFAFEF

MRDRVIMKPQFIPNVYGEHSGMPVSVNLSALGMVHPMSESGPNATVLNIEEKRQSFEH

ERKPPSRIEDKT

YHELVQSAPVISSKNDTGQRRHSLVSSRASDSSLNRAKFLCSFGGKVIPRPRDQKLRYV

GGETRIIRISK

TISFQELMHKMKEIFPEARTIKYQLPGEDLDALVSVSSDEDLQNMMEECIVFGNGGSEK

PRMFLFSSSDI

EEAQFVMEHAEGDSEVQYVVAVNGMDLSSRRSSLGLSPPGNNLDELLHGNFDRKIDR

AATEPAVASLTPL

AGNESLPASQTSQPVTGFSTGNEPFSQPYLGQQLQFPGLGNHQIYTSGHMASIGYIDE

KRSAPLHVQPQP

HYIPYSVNPETPLESLVPHYPQKPEQGFLREEQIFHVQDPETSSKEAKMRRDDSFQKVN

DHPISTVESNL

SAKEPKMRRESSTPRVNEYPVSSMPSDLIVPDDLPKEEAPIVTQTSSSTPDPSSSTLSE

KSLRKSEDHVE

NNLSAKEPKMRKEHSTTRVNEYSVSSVSSDSMVPDQALKEEAPISMKISNSTPDPKSLV

YPEKSLRTSQE

KTGAFDTTNEGMKKNQDNQFCLLGGFSVSGHGTSNNSSSNVSNFDQPVTQQRVFHSE

RTVRDPTETNRLS

KSDDSLASQFVMAQTTSDAFLPISESSETSHEANMESQNVHPTAPVIPAPDSIWTAEGS

MSQSEKKNVET

NTPEHVSQTETSAKAVPQGHNEKGDIVVDINDRFPREFLADILKTKESLNFPGLGPLHAD

GAGVSLNIQN

NDPKTWSYFRNLAQDEFERKDLSLMDQDHPGFPTSMTNTNGVPIDYSYPPLQSEKVAS

SQIHPQIHFDGN

IKPDVSTITIPDLNTVDTQEDYSQSQIKGAESTDATLNAGVPLIDFMAADSGMRSLQVIKN

DDLEELKEL

GSGTFGTVYHGKWRGTDVAIKRIKRSCFIGRSSEQERLTSEFVVHEAEILSKLHHPNVMA

FYGVVKDGPGG

TLATVTEYMVNGSLRHVLLSNRHLDRRKRLIIAMDAAFGMEYLHSKSIVHFDLKCDNLLV

NLKDPARPIC

KVGDFGLSKIKRNTLVTGGVRGTLPWMAPELLSGSSSKVSEKVDVFSFGIVLWEILTGE

EPYANMHYGAI

IGGIVNNTLRPTVPNYCDPEWRMLMEQCWAPDPFVRPAFPEIARRLRTMSSSAVHTKP

HAVNHQIHK

At5g44860，SEQ IDN o.31

＞GM47134162 agnoprotein matter

atggacagagaacaagaagagatgcaatttcttgggttctttgacatatacaaagaagcctctaagatcatactttcatggag

gaaaatcttcacccaaatcacctcaacactaatcctgcctctctccttcatcttcctaatccacatggaaatctccaacctcctttt

caggaagatcctcatcaacgaaatagtcattggacgaaacaaggcgtaacacaccccaatacaacaagcttgaccgcat

gatcttctctgaattgatcactcttgtgctcttcaaatcgcatacttcaactcttccttctcatattctctctcctttctacctcggcagtag

tctacaccatcgcatcaatctacaccgcaaaagaagtgacattcaagagggtcatgagtgttgtccctaaggtgtggaaaa

ggttaatgttgacctttctatgtgcctttgctgcttttttcatttacaatatcgtgaccatgttggttatgttcttgtcaatagtcacaatag

ggataagtagtggtggggttgtggttttggttttgataacggttttgtacttcattgggtttgtgtacctcaccgtggtgtggcagcta

gcaagtgttgtgaccgtgttggaggactcgtgggggattcgagccatggccaagagcaaggagttgataaaggggaagat

ggttttatccatattcgtctttttcacccttgtggcttcttttgtttccattagggttttgttcaaggtgatggtggttgatggatggagggt

gagttctgtggacaaaacagcatatggggttctctgttcttgctcttgtcttgtttgttcctctttgggcttgttcttcaaactgtgctct

actttgtttgcaagtcctatcaccatgagaatattgacaaatcggctttggcagatcatcttgaagggtatagaggagagtatgt

tccattgacagctaaggatgttcagctggagcaataccaagtttga

＞GM47134162 agnoprotein matter

mdreqeemqflgffdiykeaskiilswrklftqitstlilplsfiflihmeisnllfrkilineivmdetrrntpqynkldrmisselitlvlfki

ayftllifslstsavvytiasiytakevtfkrvmsvvpkvwkrlmltfcafaaffiynivtmlvmflsivtigissggvvvlvlitvlyfigf

vyltvvwqlasvvtvledswgiramakskelikgkmvlsifvfftlvasfvsirvlfkvmvvdgwrvssvdktaygvlcflllsclflf

glvlqtvlyfvcksyhhenldksaladhlegyrgeyvpltakdvqleqyqv*

＞KO18598 (gi|2660661) arabidopsis thaliana chromosome V BAC T19K24 genome sequence, complete sequence

ATGGCAGCATCTTCCGAAATACTCCCGGAGTCGTGGCAAGTGTTCATCAATTTCCGAGGAGC

AGATTTGC

GCAACGGTTTCATCAGCCATCTGGCGGGAGCTTTGACCTCAGCTGGAATCACATACTACATC

GACACGGA

AGAAGTCCCGAGCGAAGATCTCACTGTCCTTTTCAAGAGGATAGAGGAATCGGAAATCGCAC

TGTCCATC

TTCTCGAGCAATATGCTGAGTCAAAATGGTGTTTGGACGAGCTCGTGAAGATCATGGAACA

AGTAAAGA

AAGGAAAGCTCAGAATCATGCCCGTCTTCTTCAACGTGAAGCCAGAGGAGGTGAGAGAGCA

GAACGGAGA

GTTCGGACTTAAGCTTTCGGAGAAGGTAAAAGCAAACGACCCAACATACCTAATTGGGAGA

ACGCTTTG

CGGTCTGTCCCAAGCAAGATAGGCTTGAATTTGGCGAATTTTAGAAACGAGAAGGAACTCCT

TGACAAGA

TCATTGACTCCATCAAAAAGTACTTGCCCGAATTACACGAGCAAGCAGAGTAGCAGAATCT

CTAAACGG

GATCTCAAAACTCAGAGGCAAAGAATGTAGACACATTTTCGCCAAACTCCAGTGATTTTCC

ATCTACT

TCCATTGACGACGACCTCAGTATCAACTCGCCTCAGTACCAAGCCACAATTCCCCCCGCAAG

CAGGGAAG

GTGAACGTCTCAACACGATCTCTACTGTAAGTTCAACTGGTAGTATTGAACATCCTCCACCCA

ACTACGG

AATAGAACCACGCCTTAAGGAGATGGAAGAAAGTTAGATTTTGATAGCCTCGAAACTAAAAC

TGTTGGA

ATTGTTGGGATGCCTGGGATTGGTAAAACCACTCTTGCAGAAACGTTGTATAGAAAGTGGGA

ACACAAGT

TTGAGAGGAGTATGTTTTTCCCAGATGCCAGTAAGATGGCGAATGAACACGGAATGTGTTGG

CTGCAGAA

GAGATTATTGGAAGAGCTGTTGAAGGATACTAATCTCAACATAGGATATACAACGAATGAACA

TGAGTTT

TGTAAGGATGTTCTTCTCCTAAAGAAAGTTTTTCTTGTCATAGATAATGTTAGTAGCGAGGAA

CAGATCG

AAACTCTTTTTGGTAAATGGAATTGGATTAAAAATGGAAGCAAGATTGTTATTACGTCAAGTGA

TGAGTC

AATGCTCAAGGGTTTCGTTAAAGATACTTATGTAGTCCCAAGTTTGAACAGCAGAGACAGTCT

ACTGTGG

TTTACTAATCATGCATTTGGTTTGGATGATGCCCAGGGAAACTTGGTAAGTTGTCCAAACAC

TTTCTGA

ATTATGCCAAAGGCAACCCACTAGCCCTCGGAGCTTTTGGTGTAGAACTTTGTGGGAAAGAC

AAGGCTGA

TTGGGAAAAGAGAATAAAAACATTGACACTAATTTCCAATAAGATGATCCAAGATGTCTTGAG

AAGAAGG

TATGATGAACTCACAGAGAGGCAGAAAGATATTTTTCTTGACGTCGCATGTTTCTTCAAATCA

GAGAATG

AAAGTTATGTACGACACGTGGTGAATTCATGTGATTCTGAGTCTACTAAGAGTTGGGATGAAA

TAACAGA

TCTCAAAGGAAAGTTTCTTGTCAATATTTCTGGTGGTCGAGTTGAGATGCATGATATACTATG

CACATTC

GCCAAGGAACTTGCTTCACAAGCATTGACTGAAGATACAAGGGTTCATCTCAGGCTGTGGAA

CTATCAAG

ATATCATGTGGTTTCTCAACAATGAATTGGAAATGGAAAATGTCAGAGGTATTTTCTTAGACAT

GTCTAA

AGTTCCGGAGGAAATGACATTTGATGGTAACATCTTTAGCAATATGTGCAATCTTCGATATCT

CAAAATA

TACAGTTCTGTTTGCCATAAGGAAGGCGAAGGTATCTTCAAATTTGACACAGTTAGGGAAATT

CAGTTAC

CATTAGACAAGGTACGCTATCTCCACTGGATGAAATATCCATGGGAGAAACTTCCATCAGACT

TCAACCC

GGAGAATCTCGTTGATCTTGAACTGCCTTATAGCTCCATTAAGAAAGTTTGGGAGGGTGTTAA

GGATACC

CCGATACTAAAGTGGGCCAATCTAAGCTATTCAAGTAAGTTGACTAACCTTTTAGGGTTGTCA

AATGCTA

AAAATCTTGAAAGATTGAATCTTGAAGGTTGCACAAGTTTGCTTAAACTGCCCCAAGAGATGG

AGAACAT

GAAAAGTCTTGTCTTCCTGAACATGAGACGTTGCACTAGTCTCACATGTCTTCAAAGTATTAA

AGTGAGC

TCTCTGAAAATTCTCATACTCAGTGACTGCTCAAAACTTGAGGAATTTGAGGTGATTTCGGAA

AATCTGG

AAGAATTATATTTAGATGGAACTGCAATAAAGGGACTTCCTCCAGCGGCCGGGGATCTGACG

AGACTTGT

CGTCTTAAATATGGAAGGCTGTACAGAACTGGAGAGTCTTCCCAAACGTCTTGGAAAACAGA

AAGCTCTT

CAAGAACTGGTACTCTCTGGATGTTCAAAGCTCGAGAGCGTTCCAACGGACGTAAAAGACAT

GAAACATC

TACGGCTCTTATTGCTTGACGGCACAAGAATCAGAAAGATCCCGAAGATAAAGTCGCTAAAG

TGTTTGTG

CTTAAGTAGAAATATTGCAATGGTCAATCTACAAGATAATCTCAAAGATTTCTCTAATCTGAAA

TGTCTT

ctctgttctttactttgttgttcatgtctatttcactgccttatggcatctaggtagtgtgatctctgttcttgagcctgtttatggacttgct

gccatgagaaaagcttatgagcttcttaaggggaaggctaagatggctatggggttggtctttgtttacctttttgtctgtgcatta

attggaggtacttttggatcgattgtggttcatggaggaggaaagtttgggactttgactaggacccttgttggtgggttgcttgtt

ggtgttcttgtgatggtgaatttggtgggtttgttggttcagagtgtgttttattacgtttgcaagagttatcatcatcagactattgata

agacggctttgtatgatcatcttggtgggtatcttggagattatgtgcctcttaaggagcaacattcagttggagaatttagacatgt

ga

＞BN41889749 agnoprotein matter

mdlqpeelqfltipqlvqesisikkrsprtfylitlslifplsfailahslftqpilsklassdppnsdrsrhdwtvllifefsylifvfafsllst

aavvftvaslytgktvsfsytisaipkvfkrllitflwvallmfaynavffvflvilflaldmnsvglavlagvllsvlyfvvhvyftalwhl

gsvisvlepvyglaamrkayellkgkakmamglvfvylfvcaliggtfgsivvhgggkfgtltrtlvggllvgvlvmvnlvgllvqs

vfyyvcksyhhqtidktalydhlggylgdyvplksniqlenldm*

＞GM59592277 agnoprotein matter

atggatcttgccccagaagagcttcaattccttaccatccccgacatcctacgagaatcaatctcaatcccaaagcgttctcc

gaaaacattttacctcattaccctcagcctcatcttccccctctccttcgcgattctagctcattccctcttcacgcacccccttattt

cccagctgcagtcccctttcaacgacccttcccaaacctcccacgagtggaccctccttcttctaatccagttcctctacctcct

cttcctcttcgccttctccctcctctccaccgccgccgccgtcttcaccgtcgcctccctctacacctccaaggccgtctccttctc

ctccaccctctccgccatcccccgcgtcttcaagcgcctcttcctcaccttcctatgggtcaccctcctcatgatcctctacaact

ccctcatcctcctctccttggtcctcatgatcctcgccatcgacaccgacaactccctcctcctcttcctcgctatcctcatcgtcct

cactctctttttagtcgcccacgtctacatcaccgccctctggcacctcgcctccgtcgtctccgtcctcgagcccgtctacggc

ctcgccgccatgaagaagtctaccacctcctcaagggcaggctccggttcgccgctgtcctcgtctccgcctatttggtcgc

ctgcggggttatctccggtgttttcagcgtggttgtggtgcacggtggggaggactatggggttttcaccagaatcgtggtggg

agggttccttgtggggcttttggtgattgtgaacttggtggggttgttggtgcagagtgtgttttactatgtttgcaagagttatcatc

atcagggtattgataagagcgcgttgcatgatcatcttggtgggtaccttggagaatacgtgcctcttaagagcagcattcag

atggagaatttggatgtatga

＞GM59592277 agnoprotein matter

mdlapeelqfltipdilresisipkrspktfylitlslifplsfsilahslfthplisqlqspfndpsqtshewtlllliqflyllflfafsllstaaa

vftvaslytskavsfsstlsaiprvfkrlfltflwvtllmilynslillslvlmilaidtdnslllflailivltlflvahvyitalwhlasvvsvlepv

yglaamkksyhllkgrlrfaavlvsaylvacgvisgvfsvvvvhggedygvftrivvggflvgllvivntvgllvqsvfyyvcksyh

hqgidksalhdhlggylgeyvplkssiqmenldv*

At1g73490，SEQ ID No.35

＞KO20868 (gi|11120784) arabidopsis thaliana chromosome 1 BAC T9L24 genome sequence, complete sequence

ATGGACCGGAGGCTCAAGAAATGCTCGACATCCACCGATGTTGAATCAGTTCATGATGTTAG

TAAGGTCA

GTCATGAAGAACTGCGAGAATCTCAGATATCTTCCTTCGCTTCCAAAATGTCTTGAGTACCTA

AACGTAT

ATGGTTGTGAAAGACTAGAATCAGTTGAGAATCCACTGGTTGCTGATAGGTTAACGTTATTCC

TTGATAG

ATCTGAGGAATTACGTTCCACTTTCTTGTTCACTAATTGCCACAATCTGTTTCAAGATGCAAAG

GACTCA

ATCTCAACCTACGCGAAATGGAAATGCCACCGACTTGCAGTTGAATGCTACGAACAGGACAT

AGTTTCTG

GAGCTTTTTTCAACACTTGCTATCCTGGATATATAGTCCCTTCGTGGTTCGATCACCAAGCAG

TTGGATC

AGTCTTAGAGCCAAGGCTGGAACCACATTGGTATAACACTATGCTTTCTGGGATAGCTCTAT

GTGCAGTT

GTATCATTCCATGAGAACCAAGATCCGATCATCGGCAGTTTCTCAGTAAAATGCACATTGCAA

TTTGAAA

ACGAAGATGGGTCTCTTCGCTTTGATTGTGATATCGGATGTTTGAACGAACCAGGAATGATT

GAGGCAGA

CCATGTTTTTATCGGCTATGTCACTTGCTCACGTTTGAAAGATCACCACTCTATACCTATTCAT

CACCCT

ACAACTGTAAAAATGCAGTTCCACTTGACTGATGCTTGTAAAAGTAAAGTGGTGGATTGTGGG

TTCCGTT

TGATGTACACCCAGAGCCGTGGCTGTTTGTTAGAGGAAGAAGTCAACGCCAACTTCACTAAA

TTATACTT

GGGTTTATTGTAA

＞KO18598 gi|2660664|gb|AAC79135.1| agnoprotein matter [Arabidopis thaliana]

MDLAAEELQFLNIQGILRESTTIPKFSPKTFYLITLTLIFPLSFAILAHSLFTQPILQLDATP

PSDQSK

TNHEWTLLLIYQFIYVIFLFAFSLLSTAAVVFTVASLYTGKPVSFSSTMSAIPLVLKRLFITF

LWVSLMM

LVYNSVFLLFLVVLIVAIDLQSVILAVFSMVVIFVLFLGVHVYMTAWWHLASVVSVLEPIYG

IAAMKKSY

ELLNGRTNMACSMVFMYLALCGITAGVFGGVVVHGGDDFGLFTLIVVGGFLVGILVIVNL

VGLLVQSVFY

YVCKSFHHQPIDKSALHDHLGGYLGDYVPLKSSIQMENFDI

＞BN41889749 agnoprotein matter

atggatctgcagccagaagaactccagttcttgacgatccctcaactagttcaagaatccatctcaatcaagaaacgatctc

caagaaccttctacctcatcaccctctccctatcttccctctctccttcgccatcctcgctcactccctcttcactcagcccattct

ctccaagctcgcctcctccgacccacctaactccgatcgctcccgccacgactggaccgtgctcctcatattcgagttcagct

acctcatcttcgtcttcgccttctctctcctctcaaccgccgccgtagtcttcaccgttgcttctctctacaccggcaaaactgtctc

cttctcctacaccatctccgccatccccaaagtctttaaacgcctcttgatcactttcctttgggttgcactcttgatgttcgcttaca

acgctgtcttctttgttttcctagtgatactattcatagctctagacatgaacagtgtaggcttagcggtcatcgctggagttataat

CGGATCCTTTGCAGAAAGCTAAGAGAGAGTTGGATAATGTGGAAATCAAAGAAAAACAGAAG

AAGCAGAA

GAACCAAATGAAACATCTGAGAAGGAAACTAAAAAATTCAGCACCGTTTACGAAAAGTTTAA

TGATACT

ATTAAAGAACTAGACAGGGTTTCTGGAACATGTCCCATACGACCTGCCATTCCATTCACGCC

CCCAAAGG

AAAAGGTGGAACCGATATATCACAATGAGTGCAATTTCGATGATAAAGCTCATCTGGGAGTAT

CTGACAG

CGCCCTTTTTGTACAAGGATTTGATACTTCCCATCCAAGGCATGAAATCAAGACAGCATTGTG

GAATCAT

TTCTCTTCATGTGGTAAGGTCTATCTGATTTATGTTCCCATTGCGTGTTCTACCGGTGCTTCG

GTGGGAT

ATGCTTTCATTGATATGAAAAATGAAACCAAGGGGTTGACACTCAATGGAAGTCATTTGGGAG

GACGGAA

GATCGATGTTATGTTCGCCATAGATAGAGAAGAGTTTTACTTCTCTTCTAACTTAAAACACTGT

CAACGC

TGCCGTAATTATAGGCCATGGCTTGTTTTAAAAGCCATGTCAGATGCCTGCTTTGAATATCAC

CAGAGGA

TTAAACCGCGGATCGTTGGCACTCCCCATAGCAAGATTGGTCGTTTTACAGCCATTATTGGT

CGTCGCTC

TTACAGCTAG

＞KO20868 gi|11120785|gb|AAG30965.1|AC012396_1 agnoprotein matter [Arabidopis thaliana]

MDRRLKKCSTSTDVESVHDVSKVTDPLQKAKRELDNVEIKEKQKKQKNQNETSEKETK

KFSTVYEKFNDT

IKELDRVSGTCPIRPAIPFTPPKEKVEPIYHNECNFDDKAHLGVSDSALFVQGFDTSHPR

HEIKTALWNH

FSSCGKVYLIYVPIACSTGASVGYAFIDMKNETKGLTLNGSHLGGRKIDVMFAIDREEFY

FSSNLKHCQR

CRNYRPWLVLKAMSDACFEYHQRIKPRIVGTPHSKIGRFTAIIGRRSYS

At1g73480，SEQ ID No.37

＞KO20868 (gi|11120784) arabidopsis thaliana chromosome 1 BAC T9L24 genome sequence, complete sequence

ATGGCGGTGGAAACAATGTCGATGGGATCAGATTCATCAACTTTGATTCTAACATCA

GGAGCAAGCGGTC

GCGTTAGGGTACTCTTCTCGATGCGAGAGCTTAAGCGTCTCGTTACGATTATCCAAT

CGTTGATTCTTTT

CCTCCTCCTTCCGTTTCGCGTCGTCGTTTGGCGGCGGAGGACTGGTGCGGTGGTT

ATCAGAGACGATAAG

CAAGAGAGGAAGGTTTGGTCTCCTCCGCAGATCGTGGTGAGGAAGAGGAACATCG

GTGGCGAAAGCAGCG

TTTCTCCTCCGTCGGTTCCAGCTGCGGTGGTGGATGGGGAGGTTGCTGTTCGACGT

GAACTGGCGATTAA

GCGAGTTTTGGAGGATGAAGGCGGCGATGGAAGCTCCGTCAGAGATTATTCGCTAT

TCACGACGAAGAGA

GGAGATACGTTGTTTAGTCAGTCATGGTCACCTCTTTCCCCAAATCACAGGGGACTT

ATTGTTCTGCTAC

ATGGATTAAACGAGCATAGGTATAGTGATTTTGCAAAGCAGCTTAATGCTAATGGGT

TCAAGGTCTATGG

AATTGACTGGATCGGTCATGGCGGAAGTGATGGACTTCATGCTTACGTTCCTTCCCT

TGATTACGCTGTC

ACAGATTTGAAATCATTTCTTGAAAAGGTATTCACAGAGAATCCAGGACTCCCCTGT

TTCTGCTTTGGAC

ACTCAACAGGTGGAGCAATCATCCTCAAGGCTATGCTGGATCCAAAGATTGAATCTC

GAGTTTCAGGCAT

TGCATTGACTTCACCAGCTGTTGGAGTCCAACCATCCCATCCAATCTTCGCTGTTCT

TGCTCCAATCATG

GCGTTTCTACTACCCAGGTACCAAATCAGTGCAGCAAACAAGAAAGGAATGCCGGT

TTCTCGTGACCCAG

CAGCTCTCATCGCCAAATACTCTGACCCATTAGTCTTCACCGGATCCATCCGGGTTA

AAACCGGCTACGA

GATCCTTAGAATCACTGCTCACTTGCAACAGAACCTGAACAAAGTGAAAGTTCCCTT

TCTTGTGATGCAC

GGTACTGACGACACAGTTACCGATCCTAGCGCCTCAAAGAAGCTCTACGAGGAAGC

TGCCTCGTCAGACA

AATCACTCAAGCTCTACGACGGGTTGTTGCACGATCTTCTTTTTGAACCCGAACGAG

AAATCATCGCTGG

AGCCATATTAGATTGGCTAAACCAGCGGGTTTAG

＞KO20868 gi|11120787|gb|AAG30g67.1|AC012396_3 lysophospholipase homologue,

[Arabidopis thaliana] inferred

MAVETMSMGSDSSTLILTSGASGRVRVLFSMRELKRLVTIIQSLILFLLLPFRVVVWRRR

TGAVVIRDDK

QERKVWSPPQIVVRKRNIGGESSVSPPSVPAAVVDGEVAVRRELAIKRVLEDEGGDGS

SVRDYSLFTTKR

GDTLFSQSWSPLSPNHRGLIVLLHGLNEHRYSDFAKQLNANGFKVYGIDWIGHGGSDG

LHAYVPSLDYAV

TDLKSFLEKVFTENPGLPCFCFGHSTGGAIILKAMLDPKIESRVSGIALTSPAVGVQPSHP

IFAVLAPIM

AFLLPRYQISAANKKGMPVSRDPAALIAKYSDPLVFTGSIRVKTGYEILRITAHLQQNLNK

VKVPFLVMH

GTDDTVTDPSASKKLYEEAASSDKSLKLYDGLLHDLLFEPEREIIAGAILDWLNQRV

At5g22400，SEQ ID No.39

＞KO20923 (gi|2564051) arabidopsis thaliana genomic dna, No. 5 karyomit(e)s, P1 clone

：MWD9

ATGACTGAAGTTCTTCACTTTCCTTCATCTCCAAGCGCTTCTCATTCATCTTCTTCTT

CTTCTTCTTCTC

CTTCACCTTCTTCTTTATCTTACGCCTCTCGCTCTAATGCGACTCTCTTGATTAGCTC

TGACCACAACCG

GAGAAACCCAGTTGCTAGATTCGATCAAGATGTTGACTTTCATGCCTCAATCGAAGA

ACAAGATTTGAGA

AGACGGAGCAGTACCGATGGAGGAGAAGAAGACGATGGTGGGGAAGATCAGATTT

CGTTGTTGGCTCTTC

TTGTTGCCATTTTCAGGAGATCTTTGATTTCTTGCAAGAGTAACCGGAGGGAGCTTT

GTAGCATGGAGAT

TGGATGGCCTACCAATGTCAGACACGTGGCGCACGTTACCTTTGATCGTTTCAATG

GCTTCTTGGGTTTG

CCTGTTGAATTCGAGCCTGAAGTTCCTAGAAGAGCTCCAAGCGCCAGTGCAACAGT

CTTTGGGGTATCAA

CCGAATCAATGCAATTATCGTATGATTCAAGAGGCAATTGTGTACCAACCATACTATT

GCTGATGCAAAA

CTGTTTATATAGTCAAGGAGGCTTGCAGGCAGAGGGCATTTTTAGACTCACTGCTGA

GAATAGTGAGGAA

GAGGCGGTTAGGGAACAATTAAACCGAGGATTTATACCTGAGCGAATCGATGTTCA

CTGTTTGGCAGGGC

TTATCAAGGCATGGTTTAGAGAACTGCCGACAAGCGTTCTTGATTCGTTGTCGCCTG

AACAGGTGATGCA

GTGCCAAACAGAAGAGGAAATGTTGAGCTCGTTAGGCTTCTTCCACCTACAGAAG

CTGCTCTACTTGAT

TGGGCCATCAATCTAATGGCAGATGTTGTTCAGTATGAACATCTAAACAAGATGAAT

TCACGCAACATCG

CTATGGTTTTCGCACCAAATATGACACAGATGGATGATCCACTGACAGCACTGATGT

ATGCGGTTCAAGT

GATGAACTTTCTCAAGACACTAATCGAAAAAACTTTAAGAGAAAGGCAAGACTCAGT

GGTCGAGCAAGCT

CATGCATTCCCTTTAGAACCGTCTGATGAGAGTGGTCACCAAAGCCCTTCACAATCT

TTGGCTTTTAACA

CCAGTGAGCAGAGTGAAGAGACGCAATCAGACAACATCGAAAATGCTGAAAATCAG

AGTTCAAGCAGTGA

GATATCAGACGAATTAACCCTAGAGAACAATGCATGTGAACAGAGAGAAACAGACTT

TGGAAAATACAGA

ACAGGAAGATTGAGCGACTCGAGTCAACAGGTGGTGCTGAATCTAGATCCTCCAGC

TCAGTGGCCAGTGG

GCAGAACAAAGGGGTTGACCAACTTGAGCCGTGTAGGATCGAGGGTAGAGCGTAC

TGAAGCTTGGCGGTGA

＞KO20923 gi|9757821|dbj|BAB08339.1|rac gtpase activating protein [Arabidopis thaliana]

MTEVLHFPSSPSASHSSSSSSSSPSPSSLSYASRSNATLLISSDHNRRNPVARFDQDVD

FHASIEEQDLR

RRSSTDGGEEDDGGEDQISLLALLVAIFRRSLISCKSNRRELCSMEIGWPTNVRHVAHV

TFDRFNGFLGL

PVEFEPEVPRRAPSASATVFGVSTESMQLSYDSRGNCVPTILLLMQNCLYSQGGLQAE

GIFRLTAENSEE

EAVREQLNRGFIPERIDVHCLAGLIKAWFRELPTSVLDSLSPEQVMQCQTEEENVELVR

LLPPTEAALLD

WAINLMADVVQYEHLNKMNSRNIAMVFAPNMTQMDDPLTALMYAVQVMNFLKTLIEKT

LRERQDSVVEQA

HAFPLEPSDESGHQSPSQSLAFNTSEQSEETQSDNIENAENQSSSSEISDELTLENNAC

EQRETDFGKYR

TGRLSDSSQQVVLNLDPPAQWPVGRTKGLNLSRVGSRVERTEAWR

At5g22430.SEQ ID No.41

＞KO20923 (gi|2564051) arabidopsis thaliana genomic dna, No. 5 karyomit(e)s, P1 clone

：MWD9

ATGGCGAATCAAGCAGCTGCTGCAGCATTCTTCCTTTTCGCTTTAGCCGTCTTCTCC

AACTTGGAGCTCT

CAGCTTCTTCACTTGTCAGTGGCAAAGATCTCTTGCCTTGACTGCCACCGCGATTTCG

ACTTCTCAGGCAT

TAAGGTCCTCCTTAAATGCGACGGAGAGAAGAAACAAATAACCGCGGTGGCAGCTG

CAGACGGATCTTTC

CGGTCAGTGCTTCCAACGGCTGACAAAAAAGGCTCCATAAATTGTCTTGCAAAGCT

CTTGGGAGGCCCTG

AGCAACTCTATGCTCACAAACACAACTTGGTCTCTGAATTGGTCAAATCTAAACACG

ATTCCAAAGTTTT

AACTACCTCAAACCCACTTGCCTTCTCTCTCTCCTGCCCCAAACCATCCCGAGATGA

TATCGGAAGTATG

ATCGGAGATTCCAAGACTATTAATTTTCCGGGGGCAGGAGGTTTTGGATTCCCACCT

GCCAGCTTCTTTC

CCTTCTTACCAATCATTGGTATCCCATGA

＞KO20923 gi|9757824|dbj|BAB08342.1| gene I:MWD9.23～agnoprotein matter [Arabidopis thaliana]

MANQAAAAAFFLFALAVFSNLELSASSLVSGKISCLDCHRDFDFSGIKVLLKCDGEKKQI

TAVAAADGSF

RSVLPTADKKGSINCLAKLLGGPEQLYAHKHNLVSELVKSKHDSKVLTSNPLAFSLSCP

KPSRDDIGSM

IGDSKTINFPGAGGFGFPPASFFPFLPIIGIP

At5g97210，SEQ ID No.43

No. 5 karyomit(e)s of＞KO20923 gi|18425164|ref|NM_126121.1| Arabidopis thaliana

The CHR5v07142002 genome sequence

ATGAAAAGTGGAGGGAACACAAACACTAAACTCATACTTGTTCATCCATACATTCAA

AAGCAAACAAGCA

CAAATCGTCTATGGCTTCTCGCTTTCGTTTCTTTCTTCACAATCGCTTTTCTCCTAAC

TCTTCTCTACAC

CACCGACTCCATCATCTCTTCTAAAAACAACTCCGCCACCGTCTCCTCCGCCGTCAA

TTCTGCCGTCACC

ACCGCTACCATCTCTCAGTTACCAACAACAGCCATCAATGCAATGCTTCACTACGCT

TCAAGATCAAACG

ACAGCTACCACATGTCATACGGAGAGATGAAATCAATCTCCGACGTCCTCCGCCGC

TGCTCTCCGCCGTG

TAATCTCTTAGTCTTCGGTCTTACACACGAAACCCTTCTCTGGAAATCGCTAAACCA

CAACGGGCGTACA

GTTTTCATCGAAGAGAATCGTTACTACGCTGCTTACTTCGAAGAAATCCACCCGGAG

ATCGAAGTCTTCG

ATGTTCAGTACACGACCAAAGCTCGTGAGGCGCGTGAGCTTGTGTCGGCGGTTAAA

GAAGCGGCGAGGAA

CGAGTGTCGTCCAGTGCAGAATCTTCTCTTTTCAGATTGTAAATTAGGACTCAATGA

TTTGCCGAATCAT

GTATACGATGTTGATTGGGATGTGATCTTAGTTGATGGACCACGTGGCGACGGTGG

AGATGTACCGGGGA

GGATGTCGTCGATTTTCACGGCGGCGGTTCTTGCTCGGAGTAAAAAAGGCGGGAAT

CCGAAGACGCATGT

GTTTGTTCATGATTATTACAGAGATGTTGAGAGACTTTGTGGGGATGAGGTTTCTTTG

CCGGGAGAATCTT

GTGGAATCTAATGATCTGCTTGCGCACTACGTGTTGGAGAAGATGGATAAAAACAG

CACGCAGTTCTGTC

GTGGTCGTAAGAAGAAACGCTCTGTTTCTTCTCCATCGGCTTGA

＞KO20923 gi|15240242|ref|NP_201522.1 | the protein of supposition; Protein: At5g67210.1

[Arabidopis thaliana]

MKSGGNTNTKLILVHPYIQKQTSTNRLWLLAFVSFFTIAFLLTLLYTTDSIISSKNNSATVS

SAVNSAVT

TATISQLPTTAINAMLHYASRSNDSYHMSYGEMKSISDVLRRCSPPCNLLVFGLTHETLL

WKSLNHNGRT

VFIEENRYYAAYFEEIHPEIEVFDVQYTTKAREARELVSAVKEAARNECRPVQNLLFSDC

KLGLNDLPNH

VYDVDWDVILVDGPRGDGGDVPGRMSSIFTAAVLARSKKGGNPKTHVFVHDYYRDVE

RLCGDEFLCRENL

VESNDLLAHYVLEKMDKNSTQFCRGRKKKRSVSSPSA

At5g67220，SEQ ID No.45

No. 5 karyomit(e)s of＞KO20923 gi|18425165|ref|NM_126122.1| Arabidopis thaliana

The CHR5v07142002 genome sequence

ATGGCGGCGGCGATGATTTCGTCTTCCGTCGTCAGCTCATGAAACTAAATCTCTCG

AATCTCAGATTTCT

ACGTACCCGAAAATCGTTAATCTCCCAGACGCGAGCAATGACTCAAAATCCGGATC

CAAAACCTGATCCA

TCGCAGGTTCTAGACGATATCCTCTGTTCGGAGCAGCGTGATGGGCAGATTGAGGA

AACAGTCGACACAG

CGCCGGCGAGCTTGGGCTCTCCAAGTCGGGTCTTAAGCATTGATACTAGAGTAGAG

AGAGCTTGGGCACA

CTGGAAAAAACTGGGTAGACCCAAGTATATCGTTGCTCCAATGGTTGATAACTCTGA

GCTTCCGTTTAGA

TTGCTCTGCCAGAAATACGGAGCTCAGGCTGCTTATACTCCGATGTTGCATTCTAGG

ATCTTCACCGAGA

CTGAGAAGTATAGAAAATCAGGAGTTCACCACCTGTAAGGAGGACAGGCCATTGTTT

GTGCAGTTCTGTGC

TAATGATCCTGATACGTTATTGGAAGCTGCAAAGAGAGTCGAACCTTACTGCGACTA

TGTTGATATCAAT

TTAGGGTGTCCTCAGCGTATAGCGAGGCGAGGAAATTATGGTGCATTCTTGATGGA

TAATCTTCCTTTGG

TGAAATCACTTGTTGAAAAGTTAGCTCAGAACCTCAATGTTCCTGTCTCCTGTAAAAT

CCGGATCTTCCC

GAACCTGGAAGATACACTCAAGTACGCCAAGATGCTAGAAGATGCTGGTTGCTCGC

TCCTAGCTGTTCAC

GGGCGAACAAGAGATGAGAAAGACGGGAAGAAATTTAGAGCTGATTGGAGCGCAA

TCAAGGAAGTGAAAA

ACGCTATGAGAATCCCTGTCTTAGCGAATGGGAATGTAAGATGCATCGAAGATGTC

GATAACTGCATCAA

AGAGACGGGTGTTGAAGGTGTTCTCTCTGCGGAGACGCTTCTTGAAAACCCGGCG

GCCTTTGCTGGGTTT

AGAACAGCTGAATGGGCAAAAGATAACGAAGAAGAGGGATTCGTCGATGGAGGGTT

AGACCAGGGAGATT

TAGTTGTTGAGTATTTAAAGCTGTGTGAGAAGCATCCGGTTCCATGGAGGATGATTC

GATCTCACGTTCA

TAAGATGTTGGGAGAATGGTTTAGAATTCATCCACAAGTTAGAGAGCAACTTAATGC

TCAAAACATATTG

ACGTTTGAGTTTCTATACGGACTTGTGGATCAGCTAAGAGAGCTTGGAGGAAGAGT

TCCACTCTACAAGA

AAAAGAAGATAGATACTCTGACTCCACAAGACTCTCCACAAAGGGTTTAGAGAGTTG

AAACTATACGTTC

TTGATTCATTGGGTTTTATCATTTATGTTGTAACACCAAATCATCAGTATCCAAATACT

ATAGTGGTATT

TTAAAACGAATTGTTGTACCTCGAAGAGATATTTTGAAATTTTAATTGATCTGATTGAAT

TTTCAC

The protein that＞KO20923 gi|15240243|ref|NP_201523.1| infers; Protein id:At5g67220.1,

Support by cDNAigi_15146315, support [Arabidopis thaliana] by cNDA:gi_20908081

MKLNLSNLRFLRTRKSLISQTRAMTQNPDPKPDPSQVLDDILCSEQRDGQIEETVDTAP

ASLGSPSRVLS

IDTRVERAWAHWKKLGRPKYIVAPMVDNSELPFRLLCQKYGAQAAYTPMLHSRIFTETE

KYRNQEFTTCK

EDRPLFVQFCANDPDTLLEAAKRVEPYCDYVDINLGCPQRIARRGNYGAFLMDNLPLVK

SLVEKLQNLN

VPVSCKIRIFPNLEDTLKYAKMLEDAGCSLLAVHGRTRDEKDGKKFRADWSAIKEVKNA

MRIPVLANGNV

RCIEDVDNCIKETGVEGVLSAETLLENPAAFAGFRTAEWAKDNEEEGFVDGGLDQGDL

VVEYLKLCEKHP

VPWRMIRSHVHKMLGEWFRIHPQVREQLNAQNILTFEFLYGLVDQLRELGGRVPLYKK

KKIDTLTPQDSP

QRV

At1g15820，SEQ ID No.47

＞KO21621 (gi|8099275) is from the sequence of No. 1 karyomit(e) BAC of Arabidopis thaliana f7H2, complete sequence

ATGGCGATGGCGGTCTCCGGAGCTGTCCTCAGTGGGCTTGGTTCTTCGTTCCTCAC

CGGAGGCAAGAGAG

GTGCCACCGCATTGGCAAGCGGCGTAGGCACTGGAGCTCAGAGAGTTGGCAGGAA

AACTCTTATTGTCGC

TGCTGCGGCTGCTCAGCCTAAGAAATCTTGGATCCCTGCCGTTAAAGGTGGTGGCA

ACTTCCTTGACCCT

GAATGGCTCGATGGCTCGCTACCAGGAGATTTCGGGTTCGACCCATTGGGTTTGGG

GAAAGACCCGGCTT

TTCTGAAATGGTACAGAGAGGCTGAGCTGATCCATGGCCGATGGGCGATGGCAGC

GGTTCTTGGGATCTT

CGTCGGCCAGGCCTGGAGCGGTGTGGCATGGTTTGAAGCTGGAGCCCAGCCAGA

CGCGATCGCTCCCTTC

TCGTTCGGGTCGCTTCTTGGAACCCAATTGCTTCTCATGGGTTGGGTGGAGAGCAA

ACGATGGGTCGATT

TCTTCAACCCGGATTCTCAATCGGTTGAGTGGGCAACGCCATGGTCGAAGACCGCC

GAGAATTTCGCGAA

CTATACCGGCGATCAGGGATACCCCGGTGGGAGATTCTTCGATCCGTTGGGTCTCG

CCGGGAAAAACCGC

GACGGTGTTTATGAGCCGGACTTTGAGAAGCTGGAGAGGCTGAAATTGGCAGAGAT

TAAGCACTCGAGGC

TCGCAATGGTTGCCATGTTGATCTTTTACTTTGAGGCCGGGCAGGGGAAAACGCCT

CTCGGTGCTCTTGG

TTTGTGA

＞KO21621 gi|8927661|gb|AAF82152.1|AC034256_16 is identical with Arabidopis thaliana Lhcb6 protein

MAMAVSGAVLSGLGSSFLTGGKRGATALASGVGTGAQRVGRKTLIVAAAAAQPKKSWI

PAVKGGGNFLDP

EWLDGSLPGDFGFDPLGLGKDPAFLKWYREAELIHGRWAMAAVLGIFVGQAWSGVAW

FEAGAQPDAIAPF

SFGSLLGTQLLLMGWVESKRWVDFFNPDSQSVEWATPWSKTAENFANYTGDQGYPG

GRFFDPLGLAGKNR

DGVYEPDFEKLERLKLAEIKHSRLAMVAMLIFYFEAGQGKTPLGALGL

＞GM50182268 chlorophyll a/b conjugated protein CP24 precursor

atggcagctgcaacatctagtgctgtgttaaacgggtttggatctcacttcttgtgtggaggaaagaggagccatgcccttcttg

ctgctagcattggagggaaagttggtgcttctgttagtcctaaaagagttattgtggcagttgctgctgcaccaaagaagtcat

ggatccccgctgtaaaaggtggtgggagtttcatagacccagaatggcttgatggctcgctaccaggtgactatggttttgac

ccactaggactaggaaaggacccggcattcctgaaatggtatagagaagctgaactcattcatgggaggtgggcaatggc

tgcagttgtaggcatcttcattgggcaggcatggagtggagttccatggtttgaggctggagcagatcctaatgcaattgctcct

ttctcatttggctctctcttaggtacccagttgctcctaatggggtgggttgagagcaagagatgggtggacttcttcaacccag

attctcagtcagtggagtgggccactccatggtcaaaaactgctgagaactttggcaactctactggtgaacaaggctaccct

ggaggaaaattctttgaccctttgggatttgctggagctatcaaggatggcgtttacattccggatgccgacaagctagagag

actgaaattgctgagattaagcatgctaggattgctatgttggctatgctgattttctactttgaggctggccagggcaagaca

ccccttggtgctcttggcttgtaa

＞GM50182268f chlorophyll a/b conjugated protein CP24 precursor

maaatssavlngfgshflcggkrshallaasiggkvgasvspkrvivavaaapkkswipavkgggsfidpewldgslpgdy

gfdplglgkdpaflkwyreaelihgrwamaavvgifigqawsgvpwfeagadpnaiapfsfgsllgtqlllmgwveskrwv

dffnpdsqsvewatpwsktaenfgnstgeqgypggkffdplgfagaikdgvypdadklerlklaeikhariamlamlifyfe

agqgktplgalgl*

At1g15825，SEQ ID No.49

＞KO21621 (gi|8099275) is from No. 1 chromosomal BAC F7H2 sequence of Arabidopis thaliana, complete sequence

ATGATGAAAGCAAAACAACTACTCGTGGTTGGACTTTTGTTGTCTCTACTCCTTTTAA

TCATTCACACAA

CAGAGTCCATATCAGACTATGAAGTGAAGTCAAACGTTAACGTAGAAGCTTAACCG

TAGAGGAGCAAAA

GCAATCAAACAGAGGAAGACGCAGCAGTGGTAGCAGTCGTAATCGCGGACGCAGA

AGCTGCGATCCTCTG

TATCAATACTTGTTCGACACCTGTGGTCATTGGCCTTTTCCTACAACTCCTTCGCCG

GAAAACCCTTTTC

TACCATTCCAACCACCGCGTCCACCACCACGTCCGAGACCGCGTCCAAGGCCATC

CCCACGTCTACCGCC

ACCTTTGGTTCCATCACCCCCACCACCACTGCATCCAAGGCCGTCCCCATGCCCAC

CACCGCTTATGCCG

TCTCCACCGCCTTTGGTTCCATCACCACCACCACCTCCTCCTTCACCGCTCGTTCCT

TCACCTCCTCCTC

CCTCTCCGCCACCATTTTTCTTCTTCCCTTCACCGCCCCCGCCGGTGATAGTGTTTC

CGCCCCCTTTGGT

GCCGTCTCCTCCGCCGCCACTACCAGGTGGTGATCAGACGACACAACCTCCGCCG

TTATGGCTACCTCCG

CCACCATTTGGAGACGAAACGCCGCCAGTGTTCTCTCTTCCACCGCCGTTGGATGA

GTTTCCACCTATGC

CACCAATAACATGGTTGCCTCCTCCGGATGTTCCCGCCCAAACCTCGTCCGCAGAG

GCCTTTGATCAGAT

TCCTCCACTTGTTACAATAACAGAAGCAATTGAGAATCCACACAACAGTCACAGACA

CAGAGACGAAAAC

AAGAAAGGTTTAGATAGAAGGAATAGAAGAGTCAAAAGCAGAAGAAGAAGCCGAAG

TAGAAACGGAGAAG

CATTCTCAACAAGGTGTGACGTGTTTTTCCGGTGCATTTTCGGAACTTGCGGTCAAT

GGAATTTCCCGAT

TGACCCTTGTCCTCAAAACCCTTTCTTGCCACCTCCGGCGACCTTACCACCACCTCT

TCCCCTTCCGCCC

CCACCGTCACTCCCAGTCACACCTTGCTCACCACCTCCGCCTCCGATCATAGTCAA

CGGTGCACCACCAC

CACCGTGTGTTACTTGTGTACAAGTATCACCTCCACCGCCAACTCCGGTTCCTTGCT

CACCACCACCGCC

TCCTCCGATTCCGGTTCCTTGCCCACCTCCACCATCTCCACCACCACCGCCTCCTC

CGCAGCCTTGCATT

ACTTGTGTCACAGCCCCAGCACCGCCTCCTCCCCAGCCTTGCATTACTTGTGTAATA

GCCCCAGCATCAC

CTCCTCCGCAGCCTTGCATTACTTGTGTAGCAGCCCCGGAACCGCCTCCTCCCCAG

CCTTGCATAACTTG

CATCCCAGCACCAGCTTCACCGCCGCCAGTACCGCCGGTGATACCATTTGTCCCTA

CGCCGATTTTTATA

CTCCCTCCATTGCCGCCTTTATTTCCTGTTCTACCACCACCATCTGTGACGCCTTCT

CCGGTGCTACCCC

TTCCTCCACCTTCTGCGCCTCTTCCACCACCATTATCTTCCTCTCTTCCCTCACCAC

CTCTTCCATTAGT

TTTATCACCACCACCACCTCTACCTGGCGGCACGGTTTCACAGCCACCATTTACAAT

GACACCGCCTCCT

CTTTTAGGTGGTGGCGCTCCGGGAACCACAGATTCACCTCCTCCGCCTCTTTTAGG

CAGTGGCGCTCCGG

GAATCACTGGTTCCCCTCCTCCTCCTCTTTTAGGCGGTGGAGCTCCGGGAATCACT

GGTTCACCTCCTCC

TCCTCTTTTAGGCGGCGGAGCTCCGGGAATCACTGGTTCACCCCCTCCTCCTCTTT

TAGGCGGCGGAGCT

CCGGGATCACTGGTTCACCCCCTCCTCCTCTTTTAGGCGGCGGAGCTCCGGGAAT

CACTGGTTCACCTC

CTCCTCCTCTTTTTGGCGGCGGAGCTCCGGGAATCACTGGTTCACCTCCTCCACCT

CTTTTTGGCGGCGG

AGCTCCAGGAATCGCTGGTTCACCCCCTCCTCCTCTTATAGGCGGTGGTGCTCCGG

GAATCACCGTTTCT

CCTCCTCCTCTATTAGGTGGCGGAGCTCCGGGAATCACCGGTTCACCTCCTCCGCC

TCTAGTCGCAGACG

TCCCGCCCATGCCACCACTAGCATGGTTTTCGCCGCCTGATATTACTACTGGATCA

CCACCACCATCTCC

AGTTTTCCTCCTTCCTCCGCCTTTAGACCGGTCAACATTAACGCCACCAGCTGCACC

TGTAGACAATCTC

CCACCGGTTATAATCACGGGATCTCCTCCACCAGTAAACAATCTCCCACCGGATATA

GTCATCGGACAAC

CGCCACCACCTGATGTAACCATTGAACCGCCTATTGACCAGTCAACATTAACGCCA

CCAGTCATTCCCGT

GACTTTGCCTCCACCGGTTCAAGACCTTCCTTCGATTTTACCTCCCCCGGCTGATGA

GTTGCCGCCACCG

GTTCAAGAATTCCCTCCGATTTTGCCTCCACCGGTTCAAGATTTCCCCCCAATTCTC

GCTCCCCCGGCTG

ATGAGTTCCCGCCAAATTTGCCTCCACCGGTTCTAGAATTCCCTCCGATTATGCCTC

CACCGGTTCAAGA

TTTCCCGCCAATTCTCACTCCACCGGCTGAAGAGTTCCCGCCGATTTTGCCTCCAC

CGGTTCAAGAGATC

CCGCCGGTTTTCACATTACCACCGACCGTACAAGATCCACCGACAATTCCAGTATTC

TCCACACCACCAG

TCCTCGGAGATTTCCCACCCCAAACTCCCGACTTTACCACGCCGCCAGAGGTCACA

AATCCATGGCAACC

GCCGGTGACGTCATTCGCACCACCAATAGAGTCCATCCCAACAATACCGGATAATC

CGTTTCCGGTTACA

CCAAACCCGGACATGGGTTCAAATCAACCGTTTGTTGAGCTTCCTCCGCCTACTTG

GGATTCCCCGCCAT

TTAATCGTTAA

＞KO21621 gi|8927662|gb|AAF82153.1|AC034256_17

MMKAKQLLVVGLLLSLLLLIIHTTESISDYEVKSNVNVEALTVEEQKQSNRGRRSSGSSR

NRGRRSCDPL

YQYLFDTCGHWPFPTTPSPENPFLPFQPPRPPPRPRPRPRPSPRLPPPLVPSPPPPLH

PRPSPCPPPLMP

SPPPLVPSPPPPPPSPLVPSPPPPSPPPFFFFPSPPPPVIVFPPPLVPSPPPPLPGGDQT

TQPPPLWLPP

PPFGDETPPVFSLPPPLDEFPPMPPITWLPPPDVPAQTSSAEAFDQIPPLVTITEAIENPH

NSHRHRDEN

KKGLDRRNRRVKSRRRSRSRNGEAFSTRCDVFFRCIFGTCGQWNFPIDPCPQNPFLPP

PATLPPPLPLPP

PPSLPVTPCSPPPPPIIVNGAPPPPCVTCVQVSPPPPTPVPCSPPPPPPIPVPCPPPPSP

PPPPPPQPCI

TCVTAPAPPPPQPCITCVIAPASPPPQPCITCVAAPEPPPPQPCITCIPAPASPPPVPPVI

PFVPTPIFI

LPPLPPLFPVLPPPSVTPSPVLPLPPPSAPLPPPLSSSLPSPPLPLVLSPPPPLPGGTVSQ

PPFTMTTPPP

LLGGGAPGTTDSPPPPLLGSGAPGITGSPPPPLLGGGAPGITGSPPPPLLGGGAPGITG

SPPPPLLGGGA

PGITGSPPPPLLGGGAPGITGSPPPPLFGGGAPGITGSPPPPLFGGGAPGIAGSPPPPLI

GGGAPGITVS

PPPLLGGGAPGITGSPPPPLVADVPPMPPLAWFSPPDITTGSPPPSPVFLLPPPLDRSTL

TPPAAPVDNL

PPVIITGSPPPVNNLPPDIVIGQPPPPDVTIEPPIDQSTLTPPVIPVTLPPPVQDLPSILPPP

ADELPPP

VQEFPPILPPPVQDFPPILAPPADEFPPNLPPPVLEFPPIMPPPVQDFPPILTPPAEEFPPI

LPPPVQEI

PPVFTLPPTVQDPPTIPVFSTPPVLGDFPPQTPDFTTPPEVTNPWQPPVTSFAPPIESIPT

IPDNPFPVT

PNPDMGSNQPFVELPPPTWDSPPFNR

At1g15825，SEQ ID No.49

＞KO21621 (gi|8099275) is from No. 1 chromosomal BAC F7H2 sequence of Arabidopis thaliana, complete sequence

ATGATGAAAGCAAAACAACTACTCGTGGTTGGACTTTTGTTGTCTCTACTCCTTTTAA

TCATTCACACAA

CAGAGTCCATATCAGACTATGAAGTGAAGTCAAACGTTAACGTAGAAGCTTTAACCG

TAGAGGAGCAAAA

GCAATCAAACAGAGGAAGACGCAGCAGTGGTAGCAGTCGTAATCGCGGACGCAGA

AGCTGCGATCCTCTG

TATCAATACTTGTTCGACACCTGTGGTCATTGGCCTTTTCCTACAACTCCTTCGCCG

GAAAACCCTTTTC

TACCATTCCAACCACCGCGTCCACCACCACGTCCGAGACCGCGTCCAAGGCCATC

CCCACGTCTACCGCC

ACCTTTGGTTCCATCACCCCCACCACCACTGCATCCAAGGCCGTCCCCATGCCCAC

CACCGCTTATGCCG

TCTCCACCGCCTTTGGTTCCATCACCACCACCACCTCCTCCTTCACCGCTCGTTCCT

TCACCTCCTCCTC

CCTCTCCGCCACCATTTTTCTTCTTCCCTTCACCGCCCCCGCCGGTGATAGTGTTTC

CGCCCCCTTTGGT

GCCGTCTCCTCCGCCGCCACTACCAGGTGGTGATCAGACGACACAACCTCCGCCG

TTATGGCTACCTCCG

CCACCATTTGGAGACGAAACGCCGCCAGTGTTCTCTCTTCCACCGCCGTTGGATGA

GTTTCCACCTATGC

CACCAATAACATGGTTGCCTCCTCCGGATGTTCCCGCCCAAACCTCGTCCGCAGAG

GCCTTTGATCAGAT

TCCTCCACTTGTTACAATAACAGAAGCAATTGAGAATCCACACAACAGTCACAGACA

CAGAGACGAAAAC

AAGAAAGGTTTAGATAGAAGGAATAGAAGAGTCAAAAGCAGAAGAAGAAGCCGAAG

TAGAAACGGAGAAG

CATTCTCAACAAGGTGTGACGTGTTTTTCCGGTGCATTTTCGGAACTTGCGGTCAAT

GGAATTTCCCGAT

TGACCCTTGTCCTCAAAACCCTTTCTTGCCACCTCCGGCGACCTTACCACCACCTCT

TCCCCTTCCGCCC

CCACCGTCACTCCCAGTCACACCTTGCTCACCACCTCCGCCTCCGATCATAGTCAA

CGGTGCACCACCAC

CACCGTGTGTTACTTGTGTACAAGTATCACCTCCACCGCCAACTCCGGTTCCTTGCT

CACCACCACCGCC

TCCTCCGATTCCGGTTCCTTGCCCACCTCCACCATCTCCACCACCACCGCCTCCTC

CGCAGCCTTGCATT

ACTTGTGTCACAGCCCCAGCACCGCCTCCTCCCCAGCCTTGCATTACTTGTGTAATA

GCCCCAGCATCAC

CTCCTCCGCAGCCTTGCATTACTTGTGTAGCAGCCCCGGAACCGCCTCCTCCCCAG

CCTTGCATAACTTG

CATCCCAGCACCAGCTTCACCGCCGCCAGTACCGCCGGTGATACCATTTGTCCCTA

CGCCGATTTTTATA

CTCCCTCCATTGCCGCCTTTATTTCCTGTTCTACCACCACCATCTGTGACGCCTTCT

CCGGTGCTACCCC

TTCCTCCACCTTCTGCGCCTCTTCCACCACCATTATCTTCCTCTCTTCCCTCACCAC

CTCTTCCATTAGT

TTTATCACCACCACCACCTCTACCTGGCGGCACGGTTTCACAGCCACCATTTACAAT

GACACCGCCTCCT

CTTTTAGGTGGTGGCGCTCCGGGAACCACAGATTCACCTCCTCCGCCTCTTTTAGG

CAGTGGCGCTCCGG

GAATCACTGGTTCCCCTCCTCCTCCTCTTTTAGGCGGTGGAGCTCCGGGAATCACT

GGTTCACCTCCTCC

TCCTCTTTTAGGCGGCGGAGCTCCGGGAATCACTGGTTCACCCCCTCCTCCTCTTT

TAGGCGGCGGAGCT

CCGGGAATCACTGGTTCACCCCCTCCTCCTCTTTTAGGCGGCGGAGCTCCGGGAAT

CACTGGTTCACCTC

CTCCTCCTCTTTTTGGCGGCGGAGCTCCGGGAATCACTGGTTCACCTCCTCCACCT

CTTTTTGGCGGCGG

AGCTCCAGGAATCGCTGGTTCACCCCCTCCTCCTCTTATAGGCGGTGGTGCTCCGG

GAATCACCGTTTCT

CCTCCTCCTCTATTAGGTGGCGGAGCTCCGGGAATCACCGGTTCACCTCCTCCGCC

TCTAGTCGCAGACG

TCCCGCCCATGCCACCACTAGCATGGTTTTCGCCGCCTGATATTACTACTGGATCA

CCACCACCATCTCC

AGTTTTCCTCCTTCCTCCGCCTTTAGACCGGTCAACATTAACGCCACCAGCTGCACC

TGTAGACAATCTC

CCACCGGTTATAATCACGGGATCTCCTCCACCAGTAAACAATCTCCCACCGGATATA

GTCATCGGACAAC

CGCCACCACCTGATGTAACCATTGAACCGCCTATTGACCAGTCAACATTAACGCCA

CCAGTCATTCCCGT

GACTTTGCCTCCACCGGTTCAAGACCTTCCTTCGATTTTACCTCCCCCGGCTGATGA

GTTGCCGCCACCG

GTTCAAGAATTCCCTCCGATTTTGCCTCCACCGGTTCAAGATTTCCCCCCAATTCTC

GCTCCCCCGGCTG

ATGAGTTCCCGCCAAATTTGCCTCCACCGGTTCTAGAATTCCCTCCGATTATGCCTC

CACCGGTTCAAGA

TTTCCCGCCAATTCTCACTCCACCGGCTGAAGAGTTCCCGCCGATTTTGCCTCCAC

CGGTTCAAGAGATC

CCGCCGGTTTTCACATTACCACCGACCGTACAAGATCCACCGACAATTCCAGTATTC

TCCACACCACCAG

TCCTCGGAGATTTCCCACCCCAAACTCCCGACTTTACCACGCCGCCAGAGGTCACA

AATCCATGGCAACC

GCCGGTGACGTCATTCGCACCACCAATAGAGTCCATCCCAACAATACCGGATAATC

CGTTTCCGGTTACA

CCAAACCCGGACATGGGTTCAAATCAACCGTTTGTTGAGCTTCCTCCGCCTACTTG

GGATTCCCCGCCAT

TTAATCGTTAA

＞KO21621 gi|8927662|gb|AAF82153.1|AC034256_17

MMKAKQLLVVGLLLSLLLLIIHTTESISDYEVKSNVNVEALTVEEQKQSNRGRRSSGSSR

NRGRRSCDPL

YQYLFDTCGHWPFPTTPSPENPFLPFQPPRPPPRPRPRPRPSPRLPPPLVPSPPPPLH

PRPSPCPPPLMP

SPPPLVPSPPPPPPSPLVPSPPPPSPPPFFFFPSPPPPVIVFPPPLVPSPPPPLPGGDQT

TQPPPLWLLPP

PPFGDETPPVFSLPPPLDEFPPMPPITWLPPPDVPAQTSSAEAFDQIPPLVTITEAIENPH

NSHRHRDEN

KKGLDRRNRRVKSRRRSRSRNGEAFSTRCDVFFRCIFGTCGQWNFPIDPCPQNPFLPP

PATLPPPLPLPP

PPSLPVTPCSPPPPPIIVNGAPPPPCVTCVQVSPPPPTPVPCSPPPPPPIPVPCPPPPSP

PPPPPPQPCI

TCVTAPAPPPPQPCITCVIAPASPPPQPCITCVAAPEPPPPQPCITCIPAPASPPPVPPVI

PFVPTPIFI

LPPLPPLFPVLPPPSVTPSPVLPLPPPSAPLPPPLSSSLPSPPLPLVLSPPPPLPGGTVSQ

PPFTMTPPP

LLGGGAPGTTDSPPPPLLGSGAPGITGSPPPPLLGGGAPGITGSPPPPLLGGGAPGITG

SPPPPLLGGGA

PGITGSPPPPLLGGGAPGITGSPPPPLFGGGAPGITGSPPPPLFGGGAPGIAGSPPPPLI

GGGAPGITVS

PPPLLGGGAPGITGSPPPPLVADVPPMPPLAWFSPPDITTGSPPPSPVFLLPPPLDRSTL

TPPAAPVDNL

PPVIITGSPPPVNNLPPDIVIGQPPPPDVTIEPPIDQSTLTPPVIPVTLPPPVQDLPSILPPP

ADELPPP

VQEFPPILPPPVQDFPPILAPPADEFPPNLPPPVLEFPPIMPPPVQDFPPILTPPAEEFPPI

LPPPVQEI

PPVFTLPPTVQDPPTIPVFSTPPVLGDFPPQTPDFTTPPEVTNPWQPPVTSFAPPIESIPT

IPDNPFPVT

PNPDMGSNQPFVELPPPTWDSPPFNR

At5g02470，SEQ ID No.51

＞KO09008 gi|30679641:80-958 Arabidopis thaliana DPA transcription factor (At5g02470) Mbna,

Complete cds

ATGAGTATGGAGATGGAGTTGTTTGTCACTCCAGAGAAGCAGAGGCAACATCCTTC

AGTGAGCGTTGAGA

AAACTCCAGTGAGAAGGAAATTGATTGTTGATGATGATTCTGAAATTGGATCAGAGA

AGAAAGGGCAATC

AAGAACTTCTGGAGGCGGGCTTCGTCAATTCAGTGTTATGGTTTGTCAGAAGTTGG

AAGCCAAGAAGATA

ACTACTTACAAGGAGGTTGCAGACGAAATTATTTCAGATTTTGCCACAATTAAGCAA

AACGCAGAGAAGC

CTTTGAATGAAAATGAGTACAATGAGAAGAACATAAGGCGGAGAGTCTACGATGCG

CTCAATGTGTTCAT

GGCGTTGGATATTATTGCAAGGGATAAAAAGGAAATCCGGTGGAAAGGACTTCCTA

TTACCTGCAAAAAG

GATGTGGAAGAAGTCAAGATGGATCGTAATAAAGTTATGAGCAGTGTGCAAAAGAA

GGCTGCTTTTCTTA

AAGAGTTGAGAGAAAAGGTCTCAAGTCTTGAGAGTCTTATGTCGAGAAATCAAGAGA

TGGTTGTGAAGAC

TCAAGGCCCAGCAGAAGGATTTACCTTACCATTCATTCTACTTGAGACAAACCCTCA

CGCAGTAGTCGAA

ATCGAGATTTCTGAAGATATGCAACTTGTACACCTCGACTTCATAGCACACCTTTCT

CGGTCCATGATG

ATGCTTACATTTTGAAACTGATGCAAGAACAGAAGCAAGAACAGAACAGAGTATCTT

CTTCTTCATCTAC

ACATCACCAATCTCAACATAGCTCCGCTCATTCTTCATCCAGTTCTTGCATTGCTTCT

GGAACCTCAGGC

CCGGTTTGCTGGAACTCGGGATCCATTGATACTCGCTGA

＞KO09008 gi|22326573|ref|NP_195867.2|DPA transcription factor [Arabidopis thaliana]

MSMEMELFVTPEKQRQHPSVSVEKTPVRRKLIVDDDSEIGSEKKGQSRTSGGGLRQFS

VMVCQKLEAKKI

TTYKEVADEIISDFATIKQNAEKPLNENEYNEKNIRRRVYDALNVFMALDIIARDKKEIRW

KGLPITCKK

DVEEVKMDRNKVMSSVQKKAAFLKELREKVSSLESLMSRNQEMVVKTQGPAEGFTLP

FILLETNPHAVVE

IEISEDMQLVHLDFNSTPFSVHDDAYILKLMQEQKQEQNRVSSSSSTHHQSQHSSAHSS

SSSCIASGTSG

PVCWNSGSIDTR

At5g02480，SEQ ID No.53

＞KO09008 gi|30679643:590-2116 Arabidopis thaliana expressed protein (At5g02480)

MRNA, complete sequence

ATGAAAGGTTCAATTCTTACTGTTTTGTCAATGGAGAATCATCATCCGTCAACGCTTT

TATCTATGGATT

CTAGTGGCTCATCTCATGAAGAGCTTGATTTGGAGATGAACAATGGTAATAGGCAAA

TCACTCTTTATAA

TCCACCAGACATTAATCTGCCTTTGTCTGTAGGAAGAAGCTCTCCTTCTTGGAATTT

GGATTCTTGTGAT

AACATTTTGGATGTTGGTCTTAGCTCTCATGTCTATGAGACCGAGACGTTTCTCAAT

GTGGTCCCGAGTA

AAGTAGCTAAGAAGTGTTTGAAACGAGGGGATAGTATGTGGGGAGCTTGGTTTTTC

TTTAGCTTCTACTT

CAGACCGGCGTTGAATGAGAAATCCAAGTCTAAGGTCATTAGGGAAAGTGGTGGTG

GTGGAGGAGGAGGA

GGAGGATGTTTTACTGGGTTTGATAAATCTGATCTCAAGCTCGATGTTTTTCTTGTTC

AGCATGATATGG

AGAACATGTATATGTGGGCTTTTAAGGATAAACCTGAGAATGCGCTTGGGAAAATGC

AGTTGAGAAGCTA

TATGAATGGGCATTCTCGTCAAGGTGAGCGTCCGTTTCCGTTTAGTGCGGAGAAAG

GGTTTGTTCGGTCT

CACAGAATGCAGAGGAAGCATTACAGGGGACTCTCTAATCCTCAGTGTCTTCACGG

GATTGAGTTTGTGG

CTTCGCCGAGTTTGTTTGGTGTCGGTGAAGAAGATAAGAAGAGATGGATGGAGCTC

ACGGGTCGAGATTT

GAAGTTCACTATCCCTCCTGATGCTAGTGATTTCGGTTCATGGAGAAATCTTCCCAA

CACAGACATCGAG

CTAGAGAGACCAGCTCATGTTACTAAAGCAGCACCGAATAACGCCAAGAAGATTCT

CAATGGCTCCGGCT

TACATTTGACAAGCAATGCGTCTTTCAGTAGCAATGGGGACTCGTCTGATCAATCTC

CAGGAGGAGGAGT

CATCAACAACAAGAAGAGAAAAGAGTTTCTATCTCCTGGAAGCAGCGAAGAAGAAT

GCTGTTTGACTGTT

AACAACATCGAGACCCACCACGCCAAGGACCCGCCCAGTTGGGTAAACGACTTCAC

GGGAGTGATGAAGA

ATAGCTGCGGACCTGTAACTGCTGCAAAAACCGTCTATGAGGACGAAGAAGCTTAT

CTGGTCGTAATAAC

TCTACCATTTGTGGATTTGAACACCGTGAAGGTTTCATGGAGGAACAATATCACAAA

TGGAATCGTGAAG

GTCACGGGACTAAGCACTTCGAGGGCTTCGTTTGTGAAGAGACGGGACCGGACTTT

CAAGCTGGTTGATC

AGATGGCTGAGCATTGTCCTCCAGGGGAATTCATGAGGGAGATACAATTGCCGAAT

CGGATTCCGGAAGA

AGCAAATATTGAAGCATACTTTGATGGGACTGGACCAGTTTTAGAGATTGTGGTTCC

AAAATTGAGAGGA

GGAGTGGAGGAAGAACACGAGGTTAGAGTTTGTCTACGGTCACACCACCTCGGAT

GA

＞KO09008 gi|18413934|ref|NP_568100.1| expressed protein [Arabidopis thaliana]

MKGSILTVLSMENHHPSTLLSMDSSGSSHEELDLEMNNGNRQITLYNPPDINLPLSVGR

SSPSWNLDSCD

NILDVGLSSHVYETETFLNVVPSKVAKKCLKRGDSMWGAWFFFSFYFRPALNEKSKSK

VIRESGGGGGGG

GGCFTGFDKSDLKLDVFLVQHDMENMYMWAFKDKPENALGKMQLRSYMNGHSRQGE

RPFPFSAEKGFVRS

HRMQRKHYRGLSNPQCLHGIEFVASPSLFGVGEEDKKRWMELTGRDLKFTIPPDASDF

GSWRNLPNTDIE

LERPAHVTKAAPNNAKKILNGSGLHLTSNASFSSNGDSSDQSPGGGVINNKKRKEFLSP

GSSEEECCLTV

NNIETHHAKDPPSWVNDFTGVMKNSCGPVTAAKTVYEDEEAYLVVITLPFVDLNTVKVS

WRNNITNGIVK

VTGLSTSRASFVKRRDRTFKLVDQMAEHCPPGEFMREIQLPNRIPEEANIEAYFDGTGP

VLEIVVPKLRG

GVEEEHEVRVCLRSHHLG

At2g25970，SEQ ID No.59

＞KO11315 gi|30682954:66-1964 Arabidopis thaliana KH domain protein white matter (At2g25970)

MRNA, complete sequence

ATGGCGGACGAATCTCAATACTCATCGGATACTTACTCCAACAAACGCAAATACGAA

GAACCAACCGCTC

CTCCTCCATCAACTCGCAGACCTACCGGCTTCTCTTCTGGTCCGATCCCATCTGCTT

CAGTTGATCCCAC

CGCACCTACCGGTCTTCCACCTTCTTCTTACAACAGCGTTCCTCCTCCGATGGATGA

AATCCAGATTGCT

AAACAAAAAGCACAAGAAATCGCTGCTCGTCTTCTTAATAGCGCTGATGCTAAACGT

CCTCGTGTTGACA

ATGGTGCTTCTTATGATTATGGTGACAACAAAGGATTTAGCTCATATCCCTCTGAGG

GTAAGCAGATGTC

AGGGACGGTTCCGTCTTCGATACCGGTTTCGTATGGTAGCTTTCAAGGAACTACTAA

GAAGATTGATATT

CCGAATATGAGAGTTGGTGTTATCATTGGTAAAGGTGGAGAGACTATTAAGTATCTT

CAGCTTCAGTCTG

GAGCTAAGATTCAGGTTACTAGAGATATGGATGCAGACCCTAATTGTGCTACTAGGA

CTGTTGACCTAAC

TGGTACCCCTGATCAGATCTCAAAGGCTGAACAGTTGATCACTGACGTCCTTCAAGA

GGCTGAGGCAGGC

AATACAGCTGGTTCAGGTGGAGGAGGCGGCCGTAGGATGGGTGGACAAGCAGGG

GCTGATCAATTTGTTA

TGAAAATTCCGAATAACAAGGTTGGTTTGATAATTGGTAAAGGAGGTGAAACAATCA

AATCTATGCAAGC

TAAGACTGGAGCTAGAATTCAGGTTATTCCTTTACATTTGCCCCCTGGAGACCCAAC

GCCAGAACGGACT

TTGCAGATTGATGGGATAACCGAACAGATTGAACATGCTAAACAATTAGTTAATGAA

ATCATCAGTGGCG

AGAACCGTATGAGAAACTCAGCAATGGGTGGAGGCTATCCACAACAAGGTGGTTAT

CAAGCCCGCCCACC

CTCAAGCTGGGCACCACCTGGTGGTCCGCCAGCACAACCTGGTTATGGTGGTTACA

TGCAACCAGGAGCA

TATCCAGGTCCACCTCAGTATGGTCAATCACCTTACGGAAGTTACCCTCAACAAACT

TCAGCTGGTTACT

ATGATCAGTCCTCTGTGCCACCATCCCAGCAGAGCGCGCAAGGTGAGTATGATTAT

TACGGTCAGCAACA

GTCTCAGCAACCAAGCAGTGGTGGTAGCTCAGCCCCACCAACAGATACCACAGGG

TACAATTACTACCAG

CATGCTTCTGGTTATGGCCAAGCTGGTCAGGGATACCAGCAAGATGGGTATGGAGC

TTACAATGCCTCGC

AGCAATCGGGATATGGTCAAGCTGCTGGGTATGATCAACAGGGTGGTTACGGCAG

CACCACTAATCCAAG

TCAAGAGGAAGATGCATCTCAAGCCGCTCCACCATCGTCAGCTCAGTCTGGACAGG

CTGGGTATGGTACA

ACTGGTCAACAGCCGCCTGCTCAAGGTAGTACTGGTCAGGCAGGGTATGGAGCTC

CTCCAACTTCTCAGG

CTGGTTACAGCAGCCAGCCAGCAGCAGCTTACAATTCTGGGTATGGAGCACCACCA

CCTGCTTCAAAGCC

ACCGACTTATGGCCAGAGCCAGCAGTCTCCAGGTGCTCCTGGGAGCTATGGTAGT

CAGTCTGGGTATGCC

CAACCAGCAGCTTCAGGGTATGGACAACCTCCAGCGTATGGGTATGGTCAAGCGC

CACAGGGATATGGGT

CTTATGGAGGATACACACAACCTGCTGCTGGTGGAGGTTACTCTTCAGACGGGTCT

GCTGGAGCCACTGC

TGGTGGTGGTGGTGGTACACCAGCTTCACAGAGTGCTGCTCCACCTGCTGGACCG

CCCAAAGCATCCCCG

AAAAGTTGA

＞KO11315 gi|15225229|NP_180167.1|KH domain protein white matter [Arabidopis thaliana]

MADESQYSSDTYSNKRKYEEPTAPPPSTRRPTGFSSGPIPSASVDPTAPTGLPPSSYN

SVPPPMDEIQIA

KQKAQEIAARLLNSADAKRPRVDNGASYDYGDNKGFSSYPSEGKQMSGTVPSSIPVSY

GSFQGTTKKIDI

PNMRVGVIIGKGGETIKYLQLQSGAKIQVTRDMDADPNCATRTVDLTGTPDQISKAEQLI

TDVLQEAEAG

NTAGSGGGGGRRMGGQAGADQFVMKIPNNKVGLIIGKGGETIKSMQAKTGARIQVIPL

HLPPGDPTPERT

LQIDGITEQIEHAKQLVNEIISGENRMRNSAMGGGYPQQGGYQARPPSSWAPPGGPPA

QPGYGGYMQPGA

YPGPPQYGQSPYGSYPQQTSAGYYDQSSVPPSQQSAQGEYDYYGQQQSQQPSSGG

SSAPPTDTTGYNYYQ

HASGYGQAGQGYQQDGYGAYNASQQSGYGQAAGYDQQGGYGSTTNPSQEEDASQ

AAPPSSAQSGQAGYGT

TGQQPPAQGSTGQAGYGAPPTSQAGYSSQPAAAYNSGYGAPPPASKPPTYGQSQQS

PGAPGSYGSQSGYA

QPAASGYGQPPAYGYGQAPQGYGSYGGYTQPAAGGGYSSDGSAGATAGGGGGTPA

SQSAAPPAGPPKASP

KS

At3g11170，SEQ ID No.65

＞KO07848 gi|30681624:159-1499 Arabidopis thaliana omega-3-aliphatic acid desaturase, the chloroplast(id) precursor-

(FAD7) (At3g11170) mRNA, complete sequence

ATGGCGAACTTGGTCTTATCAGAATGTGGTATACGACCTCTCCCCAGAATCTACACA

ACACCCAGATCCA

ATTTCCTCTCCAACAACAACAAATTCAGACCATCACTTTCTTCTTCTTCTTACAAAAC

ATCATCATCTCC

TCTGTCTTTTGGTCTGAATTCACGAGATGGGTTCACGAGGAATTGGGCGTTGAATGT

GAGCACACCATTA

ACGACACCAATATTTGAGGAGTCTCCATTGGAGGAAGATAATAAACAGAGATTCGAT

CCAGGTGCGCCTC

CTCCGTTCAATTTAGCTGATATTAGAGCAGCTATACCTAAGCATTGTTGGGTTAAGA

ATCCATGGAAGTC

TTTGAGTTATGTCGTCAGAGACGTCGCTATCGTCTTTGCATTGGCTGCTGGAGCTG

CTTACCTCAACAAT

TGGATTGTTTGGCCTCTCTATTGGCTCGCTCAAGGAACCATGTTTTGGGCTCTCTTT

GTTCTTGGTCATG

ACTGTGGACATGGTAGTTTCTCAAATGATCCGAAGTTGAACAGTGTGGTCGGTCATC

TTCTTCATTCCTC

AATTCTGGTCCCATACCATGGCTGGAGAATTAGTCACAGAACTCACCACCAGAACC

ATGGACATGTTGAG

AATGACGAATCTTGGCATCCTATGTCTGAGAAAATCTACAATACTTTGGACAAGCCG

ACTAGATTCTTTA

GATTTACACTGCCTCTCGTGATGCTTGCATACCCTTTCTACTTGTGGGCTCGAAGTC

CGGGGAAAAAGGG

TTCTCATTACCATCCAGACAGTGACTTGTTCCTCCCTAAAGAGAGAAAGGATGTCCT

CACTTCTACTGCT

TGTTGGACTGCAATGGCTGCTCTGCTTGTTTGTCTCAACTTCACAATCGGTCCAATT

CAAATGCTCAAAC

TTTATGGAATTCCTTACTGGATAAATGTAATGTGGTTGGACTTTGTGACTTACCTGCA

TCACCATGGTCA

TGAAGATAAGCTTCCTTGGTACCGTGGCAAGGAGTGGAGTTACCTGAGAGGAGGAC

TTACAACATTGGAT

CGTGACTACGGATTGATCAATAACATCCATCATGATATTGGAACTCATGTGATACAT

CATCTTTTCCCGC

AGATCCCACATTATCATCTAGTAGAAGCAACAGAAGCAGCTAAACCAGTATTAGGGA

AGTATTACAGGGA

GCCTGATAAGTCTGGACCGTTGCCATTACATTTACTGGAAATTCTAGCGAAAAGTAT

AAAAGAAGATCAT

TACGTGAGCGACGAAGGAGAAGTTGTATACTATAAAGCAGATCCAAATCTCTATGGA

GAGGTCAAAGTAA

GAGCAGATTGA

＞KO07848 gi|15229692|ref|NP_187727.1| omega-3-aliphatic acid desaturase, the chlorophyll precursor

(FAD7) [Arabidopis thaliana]

MANLVLSECGIRPLPRIYTTPRSNFLSNNNKFRPSLSSSSYKTSSSPLSFGLNSRDGFTR

NWALNVSTPL

TTPIFEESPLEEDNKQRFDPGAPPPFNLADIRAAIPKHCWVKNPWKSLSYVVRDVAIVFA

LAAGAAYLNN

WIVWPLYWLAQGTMFWALFVLGHDCGHGSFSNDPKLNSVVGHLLHSSILVPYHGWRIS

HRTHHQNHGHVE

NDESWHPMSEKIYNTLDKPTRFFRFTLPLVMLAYPFYLWARSPGKKGSHYHPDSDLFL

PKERKDVLTSTA

CWTAMAALLVCLNFTIGPIQMLKLYGIPYWINVMWLDFVTYLHHHGHEDKLPWYRGKE

WSYLRGGLTTLD

RDYGLINNIHHDIGTHVIHHLFPQIPHYHLVEATEAAKPVLGKYYREPDKSGPLPLHLLEIL

AKSIKEDH

YVSDEGEVVYYKADPNLYGEVKVRAD

At1g77310，SEQ ID No.67

＞KO07848 gi|18411471:150-2249 Arabidopis thaliana expressed proteins (At1g77310)

MRNA, total length cds

ATGGAGGACGAACCAAAGCTCCCAACCGATGACGGTCCAACTTTCAACGAATCGTG

TAAAATCTCGTCTG

AGATATTGACCGCCGGTGATCGGAAATTACTTAAAGTTGAACTCCTCAAAGAGGAGA

CCACGCTCGTATC

GTGGAAGAAGCTTATGGATGAGGCTAGCAAAGAAAACGGCGGCTTGTTCGTTTCGG

CTCCCGAACGGCTT

CTTAATGCCAACCCTAACCTCGAGTTTCGCCTTGCACCGGGGGCACAAACAGAGAA

TGAAATGGTGAATC

AACCTCATCCTAATCGTCTTAACTCTGTTATAGCCAAGATTGAGAGACTTTATATGGG

TAAAGACGGTAG

TGATGGGGAAGAGTTAGACGGTGCTCCTGACGATGATGACTATGACACTGAAGATT

CATTTATCGATGAT

GCTGAATTGGATGAGTATTTTGAAGTTGATAATTCGCCAATTAAACATGATGGATTTT

TTGTCAATAGAG

GAAAGTTAGAACGAATTGAACCTTCAGCTACATCGAACCAGCAGCAACCAAAGAAAA

GGCGAAGGAAGGA

GTCAGCAAAACCTTGTGGCGATGTTGTTGATGTATCCAGAAAACGAGCCAAGATGG

CTAAGACGGCTGGG

GGAAAGGATCAATCTGCTTCTCCTGGGCCCTCTTCGAAGAAAATTTCCAATGATTCA

AAGACGGTGCAAG

ATTCGTTTTCCCCTTTGAAAGCGCAAAATGGCAATGATTCCTTAGTTTTGGAAAATGT

GAAGCATACTGA

TAAAGCGAATCACCAGCCAATGAATGCCACGAGTCCGAAGTCAAAGGCAGCTGGAT

CTTCTGGCCCCCTT

CATCCGAAGTGCAGCAGCAAAAGTGTTCATGAACAATCTAATTCCCCTCCAGGAAAA

TCTCGGCCAAATG

TTTCGGCAAAATCAGCAGTAGTTCGTCAGCAAGTTAACAATGGCATGCCTGACCTG

GACATTGCAACGGA

AAGCAAAACATCTATTCAAATATCTAAAAAAAGCGGTTCAAATGGCCGGCCTAAATA

CTCGACACTTGAG

AAAGCCATCAGGAATTTGGAGAAGTTGGTCGCTGAATCAAGGCCTCCTGCTGCCAC

TGAGAATCAAGATG

CCGATATCTCTTCCCAAGCAGTGAAGAGGGGATTGCCAGGAGATGTAAAATTGCAT

CTTGCTAAAGTTGC

TAGAATCGCGTATGCGAGCCAAGGTGAAATATCAGGAGAGTTAATCAATCGTCTCAT

GGGCATTGTCGGT

CATCTAATACAGATTAGATCACTTAAGGTGAAAGCTCTTCCATTCCAGAAAGAGCTA

ACAAGATCTGTAT

TTGTTAGTGAAGGAGTTCAAGCTCTTACGGAAACAAATCAAGAAGCTGGAACATCAG

ACGATTTTCAGGA

TGTTGGATCTCTTGGAAAGTCACCTGTGAAGAAGTTTGTCATGGATGTGGCGCTGG

AGGAAAAATTGTGT

GATCTATATGACGTGTTTGTTGAGGGAATGGATGAACATTCAGGTTCACAAATCAGA

AAGCTTTATTCAG

ATCTAGCTCAACTGTGGCCCAATAGTTTAGTTGACAATCATGAGATCAGGCGTGCCA

TTTGCCGGGAAAA

GGAAAGGCGGAGAGCATTGGAAGGAAACATTGGGAAGGAGATGGATCAAACGAAG

ATAACAAAGAAGAAA

CAGACACAATTGGTCCCTAAATCTGAGGGTATTACTTATCCCGACAAGACTTCAGGT

GTTGAAGTTAAAG

CAAGTGTTGTCCTAACTGCAACCACCACGTCCTTAGTGGACTGTCAACCTGCAGCA

GACTCGTCCTTTGA

AAGGTCAAAGCAGCAACATGAGAAATTAAAGCGAACTTCGAGCTTAAGCAATCCTG

CAGCAGAAGGAAAG

AAAGTCAGAAGAAAGACAGAACCAGCTCTAGAAGAAACTCACCTGCCCGCAGAGAA

ACCCCTCGTTCTGG

CCCTGAAGCGGCAGACACATCTAAAATCCAAGACACATAAACAGGTACAGGTACAT

CCACAGTCCAAGGC

ACATAAACAGGCACAGGTACATCCAAAGGCCAAGACACAGACTCCTCCAGACCTGA

ACCTGCCAAGTTAG

＞KO07848 gi|15223894|ref|NP_177855.1| expressed proteins [Arabidopis thaliana]

MEDEPKLPTDDGPTFNESCKISSEILTAGDRKLLKVELLKEETTLVSWKKLMDEASKEN

GGLFVSAPERL

LNANPNLEFRLAPGAQTENEMVNQPHPNRLNSVIAKIERLYMGKDGSDGEELDGAPDD

DDYDTEDSFIDD

AELDEYFEVDNSPIKHDGFFVNRGKLERIEPSATSNQQQPKKRRRKESAKPCGDVVDV

SRKRAKMAKTAG

GKDQSASPGPSSKKISNDSKTVQDSFSPLKAQNGNDSLVLENVKHTDKANHQPMNATS

PKSKAAGSSGPL

HPKCSSKSVHEQSNSPPGKSRPNVSAKSAVVRQQVNNGMPDLDIATESKTSIQISKKS

GSNGRPKYSTLE

KAIRNLEKLVAESRPPAATENQDADISSQAVKRGLPGDVKLHLAKVARIAYASQGEISGE

LINRLMGIVG

HLIQIRSLKVKALPFQKELTRSVFVSEGVQALTETNQEAGTSDDFQDVGSLGKSPVKKF

VMDVALEEKLC

DLYDVFVEGMDEHSGSQIRKLYSDLAQLWPNSLVDNHEIRRAICREKERRRALEGNIGK

EMDQTKITKKK

QTQLVPKSEGITYPDKTSGVEVKASVVLTATTTSLVDCQPAADSSFERSKQQHEKLKRT

SSLSNPAAEGK

KVRRKTEPALEETHLPAEKPLVLALKRQTHLKSKTHKQVQVHPQSKAHKQAQVHPKAK

TQTPPDLNLPS

At1g77320，SEQ ID No.69

＞KO07848 gi|18411482:1-2352 Arabidopis thaliana is inferred albumen (At1g77320)

MRNA, total length cds

ATGAAGACGACGCAACTGTTCAAAGGGGCAAATGTTTTTATGTCTCGGAATCTGGTG

CCTCCTGAAGTCT

TCGACACACTTCTCGATGCTTTCAAGCTTAACGGTGCCGAAATCTTCCTCTGCTGCG

ACCCATCTCGGAG

TGGTCCCTCTGATTTCCATGTCATCGCTTCTCCCGATCATGAGAAATTTAAGGATCT

TAAAGCCAAGGGT

TGTAACTTAATAGGTCCGCAATGTGCGCTCTTCTGTGCAAAAGAGGGTAGACCACT

GCCACAAAGGGGAT

TCACTTGTTGCCTAGCCATGGATGGTCTAAAAGTTCTTGCTTCTGGTTTTCTGGTAG

ATGAGAAGGTCAA

GATCAAGGAGTTGGTTACTTCCATGGGGGGCGTTTTACTTTCCAGAGCTTCTTCTGA

TGTGAACTTCGTC

ATTGTGAAAAATGTCTTGGCTGCCAAGTACAAGTGGGCCCTGAATAAGAAGCCAAT

CGTTACTCTGAATT

GGTTACATCGGTGTTGGAATGAGCACCGTGTGGTTCCTCAGGAACCATATAAGATT

CCTCCTTTTTCTGG

ATTGACAATCTGTGTCACAAGAATTCCAGCAGGTGACAAATACAAAGTTGCTCGAAA

ATGGGGTCACATT

CAAATTGTCACACGGAAATGGTTTCAGCAGTCCATCGATAAAAAGGTTTGTCTCAAT

GAAGAGTCATATC

CTGTTCTCGGTTCCATACCCTTGACAAGAGGAGTGCGAGATTTGGGGGTTCATAAT

GGTCTAGAAAAGTT

TCCTTCGGCTGCAACTGCGTCCGCGGCAGATTCATATGTTTCTTGTGCTCAGTCTAG

AGACTCAGATATA

GAAGCTTCTGCTTCACAAAATGTTTTTCCCACTTCTATGAATCCCAGTACCGATGTTA

AAGAACCAGGTG

GAGGCCCAACGGCAAGGCCGCAAGAGCAAAACATTGATGGTTGTACTGCCAGGGA

TTCAGAATCCGAAGA

CAATGACTTGTACTTATCAGATTGTAGAATTTTCTTGCTTGGTTTTGAAGCTTCTGAA

ATGCGTAAACTT

GCTAAGTTGGTCCGCAGAGGTGGTGGATCCCGGTATATGCTGCTTAACGAAAGAAT

GACTCATATTGTTG

TTGGAACTCCTTCAGAGAGAGAAGCAAGGAGTGTTGCAGCTTCTGGTGTCATTCAA

GTAGTCATACCCAG

TTGGCTTGAAGATTGTGATCGTGAGAAAAAAGAAATCCCCGTTCATATATATATACT

GCTAACCACTTG

ATTCTTCCAAGAGATTCTGCATGCTTGACCAAGGGGTCATTTGCAAGGATGTCAAGT

ATGGAACAGACTA

AAAATACTCACGACCAGACCATGGTTGGTTGTTTACTTGCTGTTAGTAGTCATATCC

TCTACTCACCTCT

TCCCTGCCAGACACCTTTGCCTGGATTCGAAAGCCTTTGCATATGTAGTTCCCAACA

TAATGAGAAGAAT

GTAGAACTCCTGAGAAATTTGAGTGTCGTTCTTGGAGCAGATTTTGTGGAAAGACTA

ACCAGGAAAGTGA

CTCACTTGATATGCAACTTTGCAAAAGGAGATAAGTATGTGAGAGCTTCCAAGTGGG

GAATAATTTCCGT

GACACCTGACTGGCTTTATGAATGTGTTAGACAGAATCAAGTTGTTTGTACAGATAA

CTTCCATCCAAGG

GAATTGACCACTCAAGATCGAGAAGCAGGGTCTCAGTTTCATACACAGTTTGTACCA

ATGGCCTCAAGGG

ACAGTATGTCTCTACCTGTAAGTCACTCTGAAGACAGGGAAAAAATTCAAAGTTTTG

CTGGCAAAAGTGG

TTGCGGGAAAGGTGAAGTATATAACAGACTTGGAGAAATTGGAAAGGAACAAACTTT

TCCGTCTAAGAAG

GCAAAACTTTTGAGAGATGGTCAAGAAAGTGATGTGTTTCCTGTGAGAGAACTTCCA

AGCAATTGTGATC

GTCCTTCGCATTCTGGAGATGGCATTGTGACTGGATATGATGTAGCAAGTGGTCGT

GAAGTTCCAGATGT

GGCTGATACTATTGAGGATCTGTTAGAGCAGACAAGCAAAATTCAAGATCAGAAGTC

TCCTGGGAGGATT

TTAGAAAAGACTGTATCCTTAAATGAACAATACAACACTGGGAATCACTCTGTCACT

GGCCTGTCTAGAC

ACTGGATAAACAGGGTCCATAAGAATGACGACATGGGCAGTCCTCCAGGAGATGCA

ACTACTGACACTTA

CGGAAACTTTAGTGAGACGCAGACAGAATCACAGGTTGTTGGTTACGAGGAAGATC

TTTCAGGAAGGCAG

ATGCTTATAGACAGAGTTAGAACACGAAGCAGCTTAACATAA

The albumen that＞KO07848 gi|15223895|ref|NP_177856.1| infers [Arabidopis thaliana]

MKTTQLFKGANVFMSRNLVPPEVFDTLLDAFKLNGAEIFLCCDPSRSGPSDFHVIASPD

HEKFKDLKAKG

CNLIGPQCALFCAKEGRPLPQRGFTCCLAMDGLKVLASGFLVDEKVKIKELVTSMGGVL

LSRASSDVNFV

IVKNVLAAKYKWALNKKPIVTLNWLHRCWNEHRVVPQEPYKIPPFSGLTICVTRIPAGDK

YKVARKWGHI

QIVTRKWFQQSIDKKVCLNEESYPVLGSIPLTRGVRDLGVHNGLEKFPSAATASAADSY

VSCAQSRDSDI

EASASQNVFPTSMNPSTDVKEPGGGPTARPQEQNIDGCTARDSESEDNDLYLSDCRIF

LLGFEASEMRKL

AKLVRRGGGSRYMLLNERMTHIVVGTPSEREARSVAASGVIQVVIPSWLEDCDREKKEI

PVHNIYTANHL

ILPRDSACLTKGSFARMSSMEQTKNTHDQTMVGCLLAVSSHILYSPLPCQTPLPGFESL

CICSSQHNEKN

VELLRNLSVVLGADFVERLTRKVTHLICNFAKGDKYVRASKWGIISVTPDWLYECVRQN

QVVCTDNFHPR

ELTTQDREAGSQFHTQFVPMASRDSMSLPVSHSEDREKIQSFAGKSGCGKGEVYNRL

GEIGKEQTFPSKK

AKLLRDGQESDVFPVRELPSNCDRPSHSGDGIVTGYDVASGREVPDVADTIEDLLEQTS

KIQDQKSPGRI

LEKTVSLNEQYNTGNHSVTGLSRHWINRVHKNDDMGSPPGDATTDTYGNFSETQTES

QVVGYEEDLSGRQ

MLIDRVRTRSSLT

At2g20210，SEQ ID No.71

＞KO28574 gi|30880916:1-816 Arabidopis thaliana is rich in leucine multiple protein family

(At2g20210) mRNA, total length cds

ATGCAACGTTTCTGTATAAAGACATCTAGCATTGAGATAGATCCACTTGCTGCGCCT

TCCGCTTTCGTTT

CATTCCTGATGTCGGTGAGGGGAAATGAACTTGACAGATACGATGCAGAGAATCTT

GCACATGCTCTACT

TCATATGCCTGGCTTGGAATCTCTTGACCTGAGCGGGAACCCCATTGAAGACAGTG

GGATCAGAAGCTTA

ATATCTTACTTCACAAAGAATCCGGATTCTCGTTTAGCCGATCTGAATTTGGAGAACT

GTGAGCTATCAT

GTTGTGGAGTTATTGAGTTTCTTGATACCCTGTCGATGCTGGAGAAACCTTTAAAGT

TCCTGTCTGTTGC

AGATAATGCCCTCGGAAGCGAGGTTGCAGAGGCTGTAGTAAACTCTTTCACAATCT

CCATCGAGTCGCTC

AATATTATGGGTATAGGACTAGGTCCTCTCGGGTTTCTTGCATTAGGCAGAAAACTT

GAAAAAGTGTCGA

AGAAGCTGCTGAGTATTAATATAAGCAAAAACCGTGGAGGACTAGAGACCGCTAGA

TTCCTGTCAAAGCT

CATACCCTTGGCACCAAAACTCATCTCAATCGACGCATCCTACAATCTTATGCCACC

TGAAGCCTTGCTC

ATGCTATGTGATTCCCTGAGAACTGCAAAAGGTGATCTCAAACGTCTTGACATGACT

GGGAATAGTTGCA

TCAGCCACGAAGCTGACCATTCTTCTCTACTCCATGAATTTCAACACAACGGAGAAC

CCATCTTCGTTTT

ACCTTCATCCTCGGTTTCACATGTTCCTTACGATGATGACCCGTAG

＞KO28574 gi|15225322|ref|NP_179611.1| is rich in leucine multiple protein family [Arabidopis thaliana]

MQRFCIKTSSIEIDPLAAPSAFVSFLMSVRGNELDRYDAENLAHALLHMPGLESLDLSGN

PIEDSGIRSL

ISYFTKNPDSRLADLNLENCELSCCGVIEFLDTLSMLEKPLFLSVADNALGSEVAEAVV

NSFTISIESL

NIMGIGLGPLGFLALGRKLEKVSKKLLSINISKNRGGLETARFLSKLIPLAPKLISIDASYNL

MPPEALL

MLCDSLRTAKGDLKRLDMTGNSCISHEADHSSLLHEFQHNGEPIFVLPSSSVSHVPYDD

DP

At5g47370，SEQ ID No.75

＞KO28574gi|30695164:263-1114 Arabidopis thaliana homeobox-leucine zipper protein

HAT2 (HD-ZIP albumen 2) is mRNA (At5g47370), total length cds

ATGATGATGGGCAAAGAAGATCTAGGTTTGAGCCTAAGCTTAGGGTTTTCACAAAAT

CACAATCCTCTTC

AGATGAATCTGAATCCTAACTCTTCATTATCAAACAATCTCCAGAGACTCCCATGGA

ACCAAACATTCGA

TCCTACATCAGATCTTCGCAAGATAGACGTGAACAGTTTTCCATCAACGGTTAACTG

CGAGGAAGACACA

GGAGTTTCGTCACCAAACAGTACGATCTCAAGCACCATTAGCGGGAAGAGAAGTGA

GAGAGAAGGAATCT

CCGGAACCGGCGTTGGCTCCGGCGACGATCACGACGAGATCACTCCGGATCGAGG

GTACTCACGTGGAAC

CTCAGATGAAGAAGAAGACGGGGGCGAAACGTCGAGGAAGAAGCTCAGGTTATCA

AAAGATCAGTCTGCT

TTTCTCGAAGAGACTTTCAAAGAACACAACACTCTCAATCCCAAACAGAAGCTAGCT

TTGGCTAAGAAGC

TGAACTTGACGGCAAGACAAGTGGAAGTGTGGTTCCAAAACAGAAGAGCTAGAACC

AAGTTAAAGCAAAC

GGAGGTAGATTGCGAATACTTGAAACGGTGCGTAGAGAAGCTAACGG AAGAGAACC

GGAGACTTCAGAAA

GAGGCTTGGAGCTTCGAACTCTCAAGCTGTCTCCACAATTCTACGGTCAGATGAC

TCCACCAACTACAC

TCATCATGTGTCCTTCGTGCGAGCGTGTGGGTGGCCCATCATCATCGAACCATCAC

CACAATCACAGGCC

CGTTTCTATCAATCCGTGGGTTGCTTGTGCTGGTCAGGTGGCTCATGGGCTGAATT

TTGAAGCCTTGCGT

CCACGATCGTGA

＞KO28574 gi|15238078|ref|NP_199548.1| homeobox-leucine zipper protein HAT2

(HD-ZIP albumen 2) [Arabidopis thaliana]

MMMGKEDLGLSLSLGFSQNHNPLQMNLNPNSSLSNNLQRLPWNQTFDPTSDLRKIDV

NSFPSTVNCEEDT

GVSSPNSTISSTISGKRSEREGISGTGVGSGDDHDEITPDRGYSRGTSDEEEDGGETSR

KKLRLSKDQSA

FLEETFKEHNTLNPKQKLALAKKLNLTARQVEVWFQNRRARTKLKQTEVDCEYLKRCV

EKLTEENRRLQK

EAMELRTLKLSPQFYGQMTPPTTLIMCPSCERVGGPSSSNHHHNHRPVSINPWVACAG

QVAHGLNFEALR

PRS

At4g33200，SEQ ID No.77

＞KO06558 gi|30689635:177-4322 Arabidopis thaliana myosin sample albumen (At4g33200)

MRNA, total length cds

ATGAGAAATTGTCTTCCAATGGAATTGAATCTGCGCAAGGGCGACAAGGTTTGGGT

CGAAGATAAGGATT

TGGCTTGGATTGCTGCTGATGTCCTCGATTCTTTTGATAACAAACTCCATGTTGAAA

CTTCTACTGGGAA

GAAGGTTTTTGTTTCCCCGGAAAAGCTATTTCGGAGGGATCCTGACGATGAAGAGC

ATAATGGAGTGGAT

GATATGACCAAACTGACATACTTGCACGAAGCTGGTGTTCTTTATAATCTACAGAGG

AGATATGCTCTGA

ATGATATCTATACATACACTGGAAGCATTCTGATCGCTGTTAATCCATTCAAAAAGCT

TCCACATCTCTA

CAATGGGCACATGATGGAACAGTACATGGGAGCACCATTCGGTGAGCTCAGTCCTC

ATGTTTTTGCAGTT

TCTGATGTTGCATACAGAGCAATGATTGACGACAGTCGAAGTCAGTCAATACTTGTT

AGCGGTGAAAGTG

GAGCTGGAAAAACTGAGACAACCAAACTAATCATGCAGTATCTTACATTTGTTGGGG

GACGTGCTACTGA

CGATGATAGAAGTGTTGAGCAGCAAGTCCTTGATCAAATCCTCTCTTGGAAGCATT

TGGCAATGCAAAA

ACAGTTAGAAATGATAATTCCAGCCGTTTTGGAAAGTTTGTCGAAATCCAGTTTGAC

ACAAATGGTAGAA

TATCTGGTGCCGCAATCAGAACCTATCTTCTGGAGAGATCACGTGTTGTCCGGATAA

CAGACCCCGAGAG

GAATTATCATTGTTTTTATCAATTGTGCGCTTCGGGGAATGACGCTGAGAAATATAAA

CTAAGCAACCCT

CGTCAATTTCATTATCTAAATCAAAGCAAGACCTATGAATTAGAAGGAGTCAGCAGC

GCAGAAGAGTATA

AGAATACAAGGAGGGCAATGGATATTGTGGGCATAAGTCAGGATGAGCAGGAAGG

GATATTTCGCACACT

TGCTGCGATTCTACATCTTGGAAATGTTGAGTTTTCCTCAGGGAGAGAGCACGACTC

TTCAGTGGTAAAG

GATCCGGAATCTAGACATCATCTGCAGATGGCTGCTGATCTTTTCAAGTGTGATGCA

AATCTTTTGCTGG

CTTCGCTCTGCACACGTTCAATTCTGACCCGTGAAGGTATCATTATCAAAGCACTTG

ACCCTAATGCTGC

TGTTACTAGCCGGGATACCCTCGCGAAGACTGTTTACGCCCATCTATTTGACTGGCT

GGTTGATAAGATC

AATAAGTCTGTTGGGCAAGATCCAGAATCTCGTTTTCAAATAGGAGTCCTGGACATT

TATGGCTTTGAAT

GTTTTAAGAATAACAGTTTTGAACATTTTGCATCAACTTTGCAAATGAAAAGCTGCA

GCAACATTTCAA

CGAGCATGTATTCAAGATGGAGCAGGATGAGTACAGAAAAGAAGAAATTAATTGGA

GTTATATCGAGTTT

ATTGACAACCAAGATGTCTTGGACCTTATTGAGAAGAAGCCTATTGGGGTGATTGCA

CTCTTAGATGAAG

CTTGCATGTTTCCTAGATCAACTCATGAGTCATTTTCAATGAAGCTGTTTCAGAACTT

TAGATTTCATCC

GAGATTGGAGAAGCCAAAATTTTCAGAGACGGATTTTACTCTCTCTCATTATGCTGG

CAAGGCAACCTTT

TTGGATAAAAACCGTGATTATACTATAGTGGAGCATTGCAATCTGCTGTCTTCCTCC

AAATGCCCTTTTG

TTGCTGGAATTTTCCCCTCAGCCCCGGAGGAGTCTACCAGATCTTCTTACAAATTTT

CTTCTGTATCTTC

CAGATTTAAGCAACAACTTCAAGCCCTCATGGAAACTCTCAGCAAAACAGAGCCTCA

CTATGTTCGGTGT

GTGAAGCCAAACTCACTCAACAGACCTCAAAAGTTTGAGAGTCTTAGTGTTTTACAT

CAACTTCGTTGTG

GGGGTGTACTGGAAGCTGTTCGGATTAGTCTAGCAGGGTATCCCACTCGAAGGAAT

TATTCAGACTTCGT

GGATCGTTTTGGTCTGCTAGCTCCAGAATTCATGGATGAGAGCAATGATGAGCAGG

CACTGACTGAGAAA

ATCTTGAGTAAATTAGGTCTTGGGAATTATCAGCTAGGAAGGACAAAAGTGTTCCTA

AGAGCTGGTCAAA

TTGGCATTTTGGACTCTAGGCGGGCTGAAGTCCTTGATGCTTCTGCAAGACTTATTC

AGCGAAGACTGAG

AACATTTGTAACGCATCAGAACTTCATCTCTGCACGGGCTTCTGCAATTTCAATTCA

GGCATACTGTAGA

GGATGCCTGTCTCGAAATGCTTATGCCACCAGAAGGAATGCGGCGGCAGCTGTCTT

GGTCCAAAAGCATG

TGCGCAGGTGGCTGTCAAGATGTGCATTTGTAAAACTTGTATCAGCTGCCATTGTAT

TACAGTCTTGCAT

CCGTGCTGACTCAACTCGCTTAAAGTTTTCACATCAGAAAGAGCATCGAGCTGCTTC

TCTAATTCAGGCT

CATTGGAGAATCCATAAGTTTCGCTCAGCATTCAGGCACCGTCAGTCATCTATTATT

GCTATTCAGTGTC

GTTGGCGACAGAAGCTTGCGAAGAGAGAGTTTAGAAAACTTAAACAGGTTGCTAAT

GAAGCAGGTGCTTT

GCGATTAGCTAAAACGAAACTTGAAAAACGGTTAGAAGATCTTGAATGGCGGTTGCA

GCTTGAGAAACGA

TTGAGAACAAGTGGTGAAGAGGCCAAGTCAAGTGAAATATCCAAGCTTCAGAAAAC

ATTGGAATCCTTCA

GCCTCAAACTAGACGCAGCTAGGCTGGCTACCATTAATGAGTGCAATAAAAATGCG

GTACTTAAAAGCA

ACTAGACATATCCATGAAGGAGAAGTCTGCTGTTGAAAGAGAGCTTAATGGAATGGT

TGAACTAAAAAAA

GATAACGCCTTGCTGAAGAATTCGATGAACTCCTTGGAAAAGAAGAATCGGGTTCTT

GAGAAGGAGCTTC

TCAATGCTAAAACCAATTGCAATAATACACTACAGAAGTTGAAGGAAGCTGAAAAAA

GGTGTTCTGAACT

CCAGACGAGTGTTCAAAGTCTTGAGGAGAAACTCTCTCATCTGGAAAACGAGAACC

AGGTCTTGATGCAA

AAGACGCTAATTACATCCCCAGAGAGAATAGGACAGATACTTGGTGAAAAACACTCT

AGTGCTGTTGTAC

CAGCCCAAAATGACAGGAGATCTGTATTTGAGAACTACGAATTGCTCTCCAGGTGTA

TAAAGGAAAATTT

GGGATTCAATGATGATAAGCCACTGGCTGCCTGTGTAATATACAAATGTCTTCTGCA

CTGGCGTGCCTTT

GAATCTGAGAGCACAGCCATATTTAACATCATTATTGAGGGAATCAATGAAGCCCTG

AAGAGAAATCTGC

GGTCAAATAGTTTTCTAAATGCAAGTGCTCAGCGTTCTGGGAGGGCTGCATATGGA

GTAAAGTCTCCTTT

TAAACTTCATGGACCTGATGATGGTGCTTCGCATATAGAAGCAAGATATCCAGCATT

ATTATTTAAACAG

CAGCTGACAGCATGTGTGGAGAAGATTTATGGTTTAATTCGTGATAATTTGAAAAAA

GAATTATCACCGC

TTCTGGGATCATGCATTCAGGTACCCTCGTTCTTCATTCGCAAACTTGTGACTCAGG

TTTTCATTCAT

CAACCTATCACTTTTCAACAGTCTTCTTCTTCGTCGTGAATGTTGCACATTTTCAAAT

GGGGAATATGTG

AAATCTGGGATTTCAGAATTGGAGAAGTGGATAGCTAATGCGAAGGAGGAGGTATT

GACTATAAGGCAAA

TATATCGAATAAGTACGATGTACTGGGATGATAAATATGGAACTCAAAGTGTCTCAA

GTGAGGTGGTTTC

TCAAATGAGGGTACTTGTGGACAAGGATAACCAAAAACAAACATCAAATTCGTTCTT

GCTGGACGATGAT

ATGAGCATTCCTTTCTCTGCAGAAGATATAGACAAGGCTATTCCAGTATTAGACCCA

TCAGAAATAGAAC

CTCCAAAATTCGTATCAGAATATACTTGTGCACAGTCCCTTGTGAAGAAACCCTCCA

TAGCTTCAACCTC

AAAGCAGATCATTTGA

＞KO06558 gi|30689636|ref|NP_195046.2| myosin sample albumen [Arabidopis thaliana]

MRNCLPMELNLRKGDKVWVEDKDLAWIAADVLDSFDNKLHVETSTGKKVFVSPEKLFR

RDPDDEEHNGVD

DMTKLTYLHEAGVLYNLQRRYALNDIYTYTGSILIAVNPFKKLPHLYNGHMMEQYMGAP

FGELSPHVFAV

SDVAYRAMIDDSRSQSILVSGESGAGKTETTTKLIMQYLTFVGGRATDDDRSVEQQVLES

NPLLEAFGNAK

TVRNDNSSRFGKFVEIQFDTNGRISGAAIRTYLLERSRVVRITDPERNYHCFYQLCASGN

DAEKYKLSNP

RQFHYLNQSKTYELEGVSSAEEYKNTRRAMDIVGISQDEQEGIFRTLAAILHLGNVEFSS

GREHDSSVVK

DPESRHHLQMAADLFKCDANLLLASLCTRSILTREGIIIKALDPNAAVTSRDTLAKTVYAH

LFDWLVDKI

NKSVGQDPESRFQIGVLDIYGFECFKNNSFEQFCINFANEKLQQHFNEHVFKMEQDEY

RKEEINWSYIEF

IDNQDVLDLIEKKPIGVIALLDEACMFPRSTHESFSMKLFQNFRFHPRLEKPKFSETDFTL

SHYAGKATF

LDKNRDYTIVEHCNLLSSSKCPFVAGIFPSAPEESTRSSYKFSSVSSRFKQQLQALMETL

SKTEPHYVRC

VKPNSLNRPQKFESLSVLHQLRCGGVLEAVRISLAGYPTRRNYSDFVDRFGLLAPEFMD

ESNDEQALTEK

ILSKLGLGNYQLGRTKVFLRAGQIGILDSRRAEVLDASARLIQRRLRTFVTHQNFISARAS

AISIQAYCR

GCLSRNAYATRRNAAAAVLVQKHVRRWLSRCAFVKLVSAAIVLQSCIRADSTRLKFSHQ

KEHRAASLIQA

HWRIHKFRSAFRHRQSSIIAIQCRWRQKLAKREFRKLKQVANEAGALRLAKTKLEKRLE

DLEWRLQLEKR

LRTSGEEAKSSEISKLQKTLESFSLKLDAARLATINECNKNAVLEKQLDISMKEKSAVERE

LNGMVELKK

DNALLKNSMNSLEKKNRVLEKELLNAKTNCNNTLQKLKEAEKRCSELQTSVQSLEEKLS

HLENENQVLMQ

KTLITSPERIGQILGEKHSSAVVPAQNDRRSVFENYELLSRCIKENLGFNDDKPLAACVIY

KCLLHWRAF

ESESTAIFNIIIEGINEALKRNLRSNSFLNASAQRSGRAAYGVKSPFKLHGPDDGASHIEA

RYPALLFKQ

QLTACVEKIYGLIRDNLKKELSPLLGSCIQVPSFFIRKLVTQVFSFINLSLFNSLLLRRECC

TFSNGEYV

KSGISELEKWIANAKEEVLTIRQIYRISTMYWDDKYGTQSVSSEVVSQMRVLVDKDNQK

QTSNSFLLDDD

MSIPFSAEDIDKAIPVLDPSEIEPPKFVSEYTCAQSLVKKPSIASTSKQII

At5g45340，SEQ ID No.79

＞KO06558 gi|30694743:83-1423 arabidopsis cell cytochrome p 450 family

(At5g45340) mRNA, total length cds

ATGGATTTCTCCGGTTTGTTTCTCACTCTCTCCGCGGCGGCTCTGTTTCTCTGTTTA

CTCCGATTTATCG

CCGGAGTCCGCCGTAGCTCCTCCACGAAACTCCCTCTTCCTCCGGGAACAATGGGT

TATCCTTACGTCGG

CGAAACATTCCAACTTTACTCACAAGACCCTAATGTGTTCTTTGCAGCAAAACAGAG

AAGATACGGATCG

GTGTTCAAGACTCATGTATTGGGATGTCCATGTGTGATGATCTCGAGCCCTGAAGC

AGCGAAATTCGTAT

TGGTTACAAAGTCTCATTTGTTTAAACCGACTTTTCCGGCCAGTAAAGAGAGGATGC

TTGGAAAACAAGC

CATCTTCTTCCATCAAGGAGATTATCATTCCAAACTTAGAAAGCTTGTTTTAAGAGCT

TTCATGCCTGAT

GCAATCAGAAACATGGTCCCTCACATTGAATCAATTGCTCAAGAATCACTCAATTCTT

GGGATGGAACTC

AACTCAACACTTACCAGGAAATGAAAACATACACTTTCAATGTTGCGTTATCTCAAT

ACTCGGCAAAGA

CGAAGTTTATTACCGAGAAGATCTAAAACGATGCTACTACATTCTAGAGAAAGGTTA

CAATTCGATGCCG

ATTAATCTTCCAGGAACATTATTCCACAAAGCCATGAAAGCTCGCAAGGAGCTAGCT

CAAATCCTCGCTA

ACATCTTATCCAAAAGAAGACAAAACCCATCATCACACACAGATCTCCTCGGATCAT

TCATGGAAGACAA

AGCAGGATTAACCGACGAACAAATCGCCGATAACATCATCGGAGTAATCTTCGCCG

CAAGAGACACGACG

GCGAGTGTTCTGACGTGGATCCTCAAGTACTTAGCTGATAATCCAACTGTTCTAGAA

GCTGTCACTGAAG

AGCAAATGGCAATAAGGAAAGATAAAAAAGAAGGAGAGAGTCTCACTTGGGAAGAT

ACAAAGAAGATGCC

ATTAACTTATAGAGTAATCCAAGAGACATTAAGAGCTGCTACAATCTTATCTTTCACA

TTTAGAGAAGCT

GTCGAAGATGTCGAATACGAAGGATATTTGATACCAAAGGGATGGAAAGTACTGCC

ACTATTCAGAAATA

TTCATCACAATGCTGATATATTTTCGGATCCGGGGAAATTCGATCCGTCGAGATTCG

AAGTTGCGCCGAA

ACCGAATACATTCATGCCTTTTGGTAGTGGGATTCATTCTTGTCCAGGCAATGAGTT

AGCTAAACTTGAA

ATCTCTGTTCTAATCCATCATCTCACCACTAAGTACAGGTTGGTACACCTTCAAAATG

ATAATAGTCCTT

TTGGGAATTGA

＞KO06558 gi|30694744|ref|NP_199347.2| Cytochrome P450 family [Arabidopis thaliana]

MDFSGLFLTLSAAALFLCLLRFIAGVRRSSSTKLPLPPGTMGYPYVGETFQLYSQDPNV

FFAAKQRRYGS

VFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHSKL

RKLVLRAFMPD

AIRNMVPHIESIAQESLNSWDGTQLNTYQEMKTYTFNVALISILGKDEVYYREDLKRCYYI

LEKGYNSMP

INLPGTLFHKAMKARKELAQILANILSKRRQNPSSHTDLLGSFMEDKAGLTDEQIADNIIG

VIFAARDTT

ASVLTWILKYLADNPTVLEAVTEEQMAIRKDKKEGESLTWEDTKKMPLTYRVIQETLRAA

TILSFTFREA

VEDVEYEGYLIPKGWKVLPLFRNIHHNADIFSDPGKFDPSRFEVAPKPNTFMPFGSGIHS

CPGNELAKLE

ISVLIHHLTTKYRLVHLQNDNSPFGN

At5g45810.SEQ ID No.81

＞KO07163 gi|18422595:1-1452 Arabidopis thaliana CBL-interaction protein kinases 19

(At5g45810) mRNA, total length cds

ATGGCGGATTTGTTAAGAAAAGTGAAATCGATAAAGAAGAAGCAGGATCAGAGCAA

TCATCAAGCTCTGA

TCCTTGGCAAATACGAAATGGGTAGGCTTCTTGGCCACGGAACCTTCGCTAAAGTC

TATCTCGCACGAAA

CGCTCAATCTGGAGAAAGCGTAGCGATCAAGGTAATTGACAAAGAGAAAGTTCTCA

AATCCGGTTTAATC

GCACACATCAAACGCGAGATCTCGATCTTGCGCCGTGTTCGTCATCCTAACATCGTT

CAGCTATTCGAAG

TCATGGCGACGAAATCTAAGATCTATTTCGTAATGGAATATGTTAAAGGAGGTGAAT

TGTTCAACAAGGT

AGCTAAAGGAAGGTTAAAAGAAGAAATGGCACGTAAATATTTTCAACAGTTGATCTC

AGCCGTATCGTTT

TGTCACTTCCGTGGTGTTTATCATCGAGATTTGAAACCGGAGAATCTTCTTTTAGAC

GAAAATGGAAACC

TAAAAGTCTCTGATTTTGGTCTTAGTGCTGTTTCTGATCAGATTCGACAAGATGGGTT

ATTTCATACTTT

TTGTGGGACCCCTGCTTACGTGGCACCGGAGGTTCTTGCTCGGAAAGGCTACGAT

GGAGCTAAAGTCGAT

ATTTGGTCTTGTGGAGTGATCTTGTTTGTGTTAATGGCAGGGTTTCTTCCTTTTCATG

ATCGGAATGTTA

TGGCTATGTATAAGAAGATTTACAGAGGAGATTTTAGGTGTCCGAGATGGTTTCCGG

TTGAGATTAACCG

GTTATTGATTCGAATGTTGGAGACTAAACCGGAGAGACGGTTTACAATGCCGGATAT

TATGGAGACTAGT

TGGTTCAAGAAAGGTTTTAAGCATATTAAGTTTTATGTTGAAGATGATCATCAGCTTT

GTAACGTTGCTG

ATGATGATGAGATCGAATCGATTGAATCGGTTTCGGGGAGGTCTTCTACGGTTTCTG

AACCGGAAGACTT

CGAGTCTTTTGATGGGAGGAGAAGAGGTGGTTCGATGCCTAGACCGGCAAGTTTGA

ATGCTTTCGATCTC

ATTTCGTTTTGCCAGGTTTTGATCTTTCGGGTTTGTTTGAGGATGATGGTGAAGGA

TCTAGGTTTGTGT

CTGGTGCTCCTGTTGGTCAGATCATTTCTAAGTTGGAGGAAATCGCGAGGATTGTG

AGTTTTACTGTGCG

AAGAAGGATTGTAAAGTGAGTCTTGAAGGTTCAAGAGAAGGAAGTATGAAAGGTC

CATTGTCAATTGCT

GCTGAGATATTTGAACTGACACCAGCTTTGGTTGTTGTTGAAGTGAAGAAGAAAGGA

GGTGATAAAATGG

AGTATGATGAGTTTTGTAATAAGGAGTTGAAACCTAAGTTGCAGAATTTGTCTTCCG

AAAATGGCCAACG

GGTTTCTGGTTCGCGTTCTTTGCCATCGTTTTTACTTTCTGATACTGATTAG

＞KO07163 gi|15242507|ref|NP_199393.1|CBL-interaction protein kinases 19[Arabidopis thaliana]

MADLLRKVKSIKKKQDQSNHQALILGKYEMGRLLGHGTFAKVYLARNAQSGESVAIKVI

DKEKVLKSGLI

AHIKREISILRRVRHPNIVQLFEVMATKSKIYFVMEYVKGGELFNKVAKGRLKEEMARKY

FQQLISAVSF

CHFRGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDGLFHTFCGTPAYVAPEVL

ARKGYDGAKVD

IWSCGVILFVLMAGFLPFHDRNVMAMYKKIYRGDFRCPRWFPVEINRLLIRMLETKPER

RFTMPDIMETS

WFKKGFKHIKFYVEDDHQLCNVADDDEIESIESVSGRSSTVSEPEDFESFDGRRRGGS

MPRPASLNAFDL

ISFSPGFDLSGLFEDDGEGSRFVSGAPVGQIISKLEEIARIVSFTVRKKDCKVSLEGSRE

GSMKGPLSIA

AEIFELTPALVVVEVKKKGGDKMEYDEFCNKELKPKLQNLSSENGQRVSGSRSLPSFLL

SDTD

At5g45820，SEQ ID No.83

＞KO07163 gi|18422596:1-1320 Arabidopis thaliana CBL-interaction protein kinases 20

(At5g45820) mRNA, total length cds

ATGGATAAAAACGGCATAGTTTTGATGCGAAAATATGAATTAGGTCGTCTTCTAGGT

CAAGGCACATTCG

CAAAAGTGTACCACGCACGCAACATAAAAACAGGAGAAAGCGTAGCGATCAAGGTG

ATCGACAAACAGAA

AGTTGCGAAAGTCGGATTAATCGATCAAATCAAACGAGAAATATCAGTGATGCGTCT

CGTTCGTCACCCC

CACGTCGTCTTCCTCCATGAAGTAATGGCGAGCAAGACAAAGATCTATTTCGCTATG

GAATACGTTAAAG

GCGGTGAGCTTTTTGATAAAGTCTCTAAAGGAAAAGCTTAAAGAAAACATTGCTCGAA

AATATTTCCAGCA

ATTGATCGGAGCAATCGATTATTGCCATAGCCGCGGAGTTTACCACCGCGATCTCA

AACCGGAGAATCTT

CTTCTAGACGAAAACGGCGATTTGAAAATATCGGATTTTGGCCTTAGCGCGTTGAG

GGAGTCGAAGCAGC

AAGATGGCTTGCTTCACACGACATGTGGAACACCTGCTTACGTGGCACCTGAAGTG

ATAGGCAAGAAAGG

TTATGATGGAGCTAAAGCCGATGTTTGGTCTTGCGGGGTTGTGTTGTACGTGCTATT

GGCTGGATTTCTT

CCGTTTCACGAGCAAAATCTTGTGGAAATGTATCGGAAAATCACGAAAGGCGAATTC

AAATGTCCGAATT

GGTTTCCTCCCGAGGTCAAGAAGTTGTTGTCTCGGATTCTTGACCCTAACCCTAATT

CAAGAATCAAGAT

TGAAAAAATCATGGAGAATTCCTGGTTTCAAAAGGGTTTCAAGAAGATCGAAACGCC

TAAATCTCCCGAA

AGTCATCAGATCGACTCACTGATCAGCGATGTCCACGCAGCTTTTTCCGTAAAACCG

ATGTCTTACAACG

CGTTTGACTTGATCTCTTCGCTGTCTCAAGGATTCGATCTCTCGGGTTTGTTTGAGA

AAGAAGAGAGATC

AGAATCGAAGTTTACAACGAAGAAAGATGCAAAAGAGATAGTGTCGAAATTCGAGG

AGATAGCAACAAGT

AGTGAGAGATTCAATTTGACGAAGAGCGATGTAGGAGTGAAGATGGAAGATAAGAG

AGAAGGAAGAAAAG

GACATCTTGCGATTGATGTTGAGATATTTGAAGTGACAAATAGTTTTCATATGGTTGA

GTTTAAGAAAAG

TGGAGGTGATACAATGGAGTATAAGCAATTTTGTGATCGTGAGCTTAGGCCTTCTTT

GAAAGATATTGTT

TGGAAATGGCAAGGAAACAACAACAATAGCAACAATGAGAAGATTGAAGTGATACAT

TAA

＞KO07163 gi|15242509|ref|NP_199394.1|CBL-interaction protein kinases 20[Arabidopis thaliana]

MDKNGIVLMRKYELGRLLGQGTFAKVYHARNIKTGESVAIKVIDKQKVAKVGLIDQIKREI

SVMRLVRHP

HVVFLHEVMASKTKIYFAMEYVKGGELFDKVSKGKLKENIARKYFQQLIGAIDYCHSRGV

YHRDLKPENL

LLDENGDLKISDFGLSALRESKQQDGLLHTTCGTPAYVAPEVIGKKGYDGAKADVWSC

GVVLYVLLGFL

PFHEQNLVEMYRKITKGEFKCPNWFPPEVKKLLSRILDPNPNSRIKIEKIMENSWFQKGF

KKIETPKSPE

SHQIDSLISDVHAAFSVKPMSYNAFDLISSLSQGFDLSGLFEKEERSESKFTTKKDAKEIV

SKFEEIATS

SERFNLTKSDVGVKMEDKREGRKGHLAIDVEIFEVTNSFHMVEFKKSGGDTMEYKQFC

DRELRPSLKDIV

WKWQGNNNNSNNEKIEVIH

At2g02370，SEQ ID NO.85

＞KO00025 gi|30677992:207-1169 Arabidopis thaliana expressing protein (At2g02370)

MRNA, total length cds

ATGTCAAACCCATTGAAAGAGTCAAGAGAGGATATTGCAAATTCTACTCCTCACATG

AGGGATAATGAGT

ATGTTCGGCTAGTTGTGGCTCATGAAGCCTCCCCAGCTGAAACCGTGTTGTCTCTAT

CGCAATCAGAGGT

GCAGAGTAAGAAATTTATGTGGTGGTTAAAAGCTTTGGGAATATGTGCAGTTGCTCT

CTTGCTTACGCTT

GTTTTCGGAAAATGGGGAGTTCCGTTTGTGTTTCAAAAGGTTCTTATTCCAATTTTGC

AATGGGAAGCAA

CTGCGTTTGGCCGTCCTATGCTCGCGATTGTCCTTGTTGTTTCCTTGGCTTTGTTTC

CTGTGTTCTTGAT

ACCTTCTGGTCCTTCCATGTGGTTAGCTGGGATGATTTTTGGTTATGGTCTCGGTTT

TGTTATTATCATG

GTTGGAACCACCATTGGCATGGTTCTCCCTTACTTAATCGGGCTTATGTTCCGTGAT

CGCCTCCATCAAT

GGTTAAAAAGATGGCCTCGTCAAGCTGCTGTTCTAAGACTAGCTGCAGAAGGAAGC

TGGTTCCATCAATT

CAGAGTCGTGGCAATCTTTCGGGTTTCCCCATTTCCTTACACGATTTTTAACTACGC

AATCGTCGTGACA

AGCATGAGATTCTGGCCTTACTTCTTCGGATCCATAGCAGGAATGATACCAGAAGCT

TTCATCTACATTT

ACAGCGGTCGGTTAATCAGAACATTCGCAGATGTGCAATACGGACATCAACGTTTG

ACAACAGTGGAGAT

TGTGTACAATGTAATCTCCTTAGTCATTGCGGTTGTGACCACTGTTGCTTTCACTGT

GTACGCGAAAAGA

GCTTTGAGAGAGCTTCAAAACGCAGAAGCTAATGAAGATGAAGAAGTTCAAGTAAG

AAAAGTGAGATTCG

AGATGAAGAACGTAGTTCAGCACGAAGAAGATAATCATCAGCGTTTGCCTTAG

＞KO00025 gi|18395356|ref|NP_565283.1| expressing protein [Arabidopis thaliana]

MSNPLKESREDIANSTPHMRDNEYVRLVVAHEASPAETVLSLSQSEVQSKKFMWWLKA

LGICAVALLLTL

VFGKWGVPFVFQKVLIPILQWEATAFGRPMLAIVLVVSLALFPVFLIPSGPSMWLAGMIF

GYGLGFVIIM

VGTTIGMVLPYLIGLMFRDRLHQVLKRWPRQAAVLRLAAEGSWFHQFRVVAIFRVSPF

PYTIFNYAIVVT

SMRFWPYFFGSIAGMIPEAFIYIYSGRLIRTFADVQYGHQRLTTVEIVYNVISLVIAVVTTV

AFTVYAKR

ALRELQNAEANEDEEVQVRKVRFEMKNVVQHEEDNHQRLP

At5g39460，SEQ ID No.87

＞KO02173 gi|18421868:1-1716 Arabidopis thaliana F-box protein family (At5g39460)

MRNA, total length cds

ATGATGAACAAGGAATCGTTTGGAGCTTGCTTGCTTCTTACGCTTCCCGAAGATGTG

TTTGCTGTTATCT

CTCGTTTTCTTTCTCCAAGCGACATTTGCAATCTAATCTTGTGCGGCAAAAGTCTTTG

TGCCCTTGTCGA

TTCCGAGAAGACGTGGCTTGTGCAATGTGAAGAAGTAAAAGTTCTTCCTTTGATTGA

ACTAGTCCAATGG

CGAATCGGGATCTCTTCTTACAAGGCCCTTTGTAGGTTTCTTGTGGAGGTGGTGAA

GCCGCTTCTTGGGA

TTTGGGTGCAAGAAAACCCTGAACTTGGGAATGTTGTTTATGTGATGCCTGGTTTCT

TGTCTGTTGTTGG

GTGCCGGATAATTCCACAAAAGGTTGCTCCTTTGTGGATTCAAGAGGGCCAAGTCA

AGTGGTCACCGGTG

TTTGAGATAATTTGCGGCTTTGATGGCTCTAAGGGTTTTTTCCTCCATGGAAGAGAC

AAACAAGGTAGTT

TCTTATACCCTGGTTTCGTTATGGACATCGAGAAGAGTTGCAATGTGCTTCTACTCG

AAGTTGAGCCGAG

GTCAGAGAAGAGTTCGTGCAATGAGATTGAGAGAGAAGTAGGGGATCCATTTGGAG

ATCTAGACTTCAGT

GATAGAATGAACTTACTAGATATAGTGACAAAACATGTAAGTCTACGAGTCGATGAA

CCATTAACAGGAA

ATTTATTTCCCACCAGGTCAAAATATGACGAAGCGATGATGTTGGAACGCAGAAACA

TGCTCCTTAAAAT

GCTCAAATTTGGTGGAAACTGGAAGCACATAAACTTGGAGGAGGATGAGCAGTTGT

GTTACAATCATATA

GAGATAGACATAAAAAAATTGTTGGAAAATCTTGGTGATGACATTGACAACATGGAG

GATATAGAGGATC

AGATAGAGGTTACACCAAGGAAGAAGAGCTTTCGCCGGTTTTTAAGAAGTGGCATT

AAACATATTCTTGG

GAAGTTCAGTTCTTCAAAGATCAATTCGCCTTCGAGCAGTGAGACAAGACGTTCGAA

TCGCCAAAGCTTT

CTCAGCTCTGGTAATACATTTTGCCTTAGTCTTAAAGCTTCATGCACTTTGATGTCTT

CATATGAAGGGT

GGCCAATCATGAGCGCAGACAACTTTTCCCTTCATAAACTACCAATGAAGAAACCTC

TCGATCACGACGT

GTATGCGGGTTTGTGGGGAGGAACGTTTGGCTGGCCCCCTGGGAAAGATATTGAA

GATGAGTCCCTTCTC

TTATTAATGCTCACTTATGGAGAATCTGAAGAGGGTAGTGAGAGAATTCTTTTCGGG

ACGAAAATACTCA

GTTATTTTGCTGAGCATCCTAATGGATCCTCAATGTTTGTTGTAAATATTGACACGCC

TTCCCTTGAGCC

GTTTCCATTTGATACAGATGGAAGAGATTTCGAGCATTCTTACACGGGAGAGGGTAT

CGCTGACGGTTAT

GGATTCCGATACCCCGGTTCAAAACCTGGTTCCCTTTTCGTAAGCTCTAATGATCTT

CTTGCATTCGTTT

GGCAAGGAACTGAAGATGTGATTACATTGCAAAGAATAAACCTTGGAGAGATCTTGA

AGAAGAGTTTAGG

TTCTTGTGTTTCACCTTTGCTTCCAACAAAGAATTTTACATATACTAAAAGGTCTTACT

CAAACGTGTTT

GCCAAGTCATCGACCTATTCGTCTTCCTCCGAGTAA

＞KO02173 gi|15241752|ref|NP_198762.1|F-box protein family [Arabidopis thaliana]

MMNKESFGACLLLTLPEDVFAVISRFLSPSDICNLILCGKSLCALVDSEKTWLVQCEEVK

VLPLIELVQW

RIGISSYKALCRFLVEVVKPLLGIWVQENPELGNVVYVMPGFLSVVGCRIIPQKVAPLWI

QEGQVKWSPV

FEIICGFDGSKGFFLHGRDKQGSFLYPGFVMDIEKSCNVLLLEVEPRSEKSSCNEIEREV

GDPFGDLDFS

DRMNLLDIVTKHVSLRVDEPLTGNLFPTRSKYDEAMMLERRNMLLKMLKFGGNWKHIN

LEEDEQLCYNHI

EIDIKKLLENLGDDIDNMEDIEDQIEVTPRKKSFRRFLRSGIKHILGKFSSSKINSPSSSET

RRSNRQSF

LSSGNTFCLSLKASCTLMSSYEGWPIMSADNFSLHKLPMKKPLDHDVYAGLWGGTFG

WPPGKDIEDESLL

LLMLTYGESEEGSERILFGTKILSYFAEHPNGSSMFVVNIDTPSLEPFPFDTDGRDFEHS

YTGEGIADGY

GFRYPGSKPGSLFVSSNDLLAFVWQGTEDVITLQRINLGEILKKSLGSCVSPLLPTKNFT

YTKRSYSNVF

AKSSTYSSSSE

At1g16540 F19K19，13，SEQ ID No.91

＞KO108276 (gi|9954737) arabidopsis thaliana chromosome I BACF19K19 genome sequence, full length sequence

ATGGAAGCATTTCTTAAGGAATTCGGAGATTATTATGGATACCCAGATGGTCCCAAG

AACATTCAAGAGA

TCCGCGACACCGAATTCAAGAGATTAGATAAAGATTACAGTTGCTTATTCACCTCCG

GAGCCACAGCAGC

GCTGAAGCTTGTCGGAGAGACTTTTCCGTGGACCCAAGACAGTAATTTTTTGTATAC

CATGGAGAATCAC

AACAGTGTACTTGGTATTAGGGAATATGCATTAGCTCAAGGTGCTTCAGCATGTGCA

GTGGATATTGAAG

AGGCAGCTAACCAACCAGGCCAGCTTACAAATTCAGGACCATCTATCAAGGTAAAG

CATCGTGCTGTGCA

GATGAGAAACACTTCTAAACTCCAAAAGGAAGAGTCAAGAGGAAATGCCTATAATCT

ATTTGCTTTCCCC

TCGGAGTGCAATTTTTCTGGCCTGAGGTTTAATCTAGATCTGGTGAAGTTGATGAAA

GAAAATACTGAGA

CCGTGCTACAAGGCTCCCCCTTTAGCAAAGAGCAAGCGGTGGATGGTCTTGATTGAT

GCTGCAAAGGGTTG

TGCTACACTACCACCTGATTTATCGGAGTATCCTGCAGATTTTGTTGTTCTGTCATTC

TACAAGTTGTGT

AAAATGGTTGAATTTGTATGGCATTTGATGAACATAATACTTACAGGCACTGTTGCTG

CTTCAATTGCTG

ACATCGACTTTGTAAAAAGAAGGGAAAGGGTGGAGGAGTTTTTTGAGGATGGTTCT

GCTTCATTCCTGAG

CATAGCAGCCATCCGTCATGGCTTCAAATTACTCAAGTCGCTTACACCTTCTGCAAT

TTGGATGCACACA

ACGTCACTTTCCATATATGTGAAAAAGAAGCTTCAGGCTTTACGACATGGAAACGGG

GCTGCTGTATGTG

TTCTGTATGGCAGTGAAAATCTGGAGTTATCTTCACATAAATCAGGCCCAACGGTTA

CATTCAACTTGAA

AAGACCTGATGGCTCTTGGTTTGGCTACTTGGAGGTGGAGAAGCTTGCTTCTTTATC

TGGAATTCAGTTA

CGGGCTGGGCATATTTGCTGGGATGACAATGATGTGATAAATGGAAAACCAACAGG

GGCTGTTAGGGTTT

CGTTTGGTTATATGTCAACCTTTGAAGATGCCAAGAAATTTATTGATTTCATCATAAG

TTCATTTGCTTC

ACCTCCAAAGAAGACTGGGAATGGAACCGTCGTCAGTGGAAGGTTTCCTCAACTTC

CTAGTGAAGACCTT

GAAAGTAAAGAATCTTTTCCAAGCCACTACCTTAAGTCAATTACTGTATACCCGATCA

AGTCATGTGCTG

GATTTTCTGTGATACGTTGGCCACTTTGCAGAACAGGCCTGCTGCATGATCGAGAAT

GGATGGTTCAGGG

TCTGACCGGTGAAATTCTTACCCAAAAGAAGGTGCCTGAGATGTCTCTTATAAAAAC

CTTTATCGACCTT

GAGGAAGGACTACTGTCTGTAGAATCTTCTCGCTGCGAAGACAAGTTGCACATCAG

AATCAAGTCTGATT

CATATAACCCGAGGAACGATGAGTTTGATTCACATGCCAACATACTTGAAAACCGTA

ATGAGGAAACTAG

AATCAATCGTTGGTTCACCAATGCCATTGGTCGACAATGCAAGTTGCTACGGTATTC

TAGCTCTACTTCC

AAAGACTGCTTGAACAGAAACAAGAGTCCTGGTTTGTGCAGAGATTTGGAAAGCAAT

ATCAACTTTGCTA

ATGAAGCTCAGTTCTTGTTAATCTCCGAGGAGAGTGTTGCTGACCTAAACAGAAGAT

TAGAAGCAAAAGA

CGAGGATTACAAACGGGCTCATGAAAAACTCAATCCACATAGGTTCAGACCAAATCT

GGTTATATCTGGA

GGTGAACCATACGGGGAAGATAAATGGAAAACTGTCAAGATAGGAGACAATCATTT

CACAGGAAAGATCT

TGTTTGGAACGCTTTTGAGATACGAGATTGATGAGAAAAGACAATGTTGGATTGGAG

TTGGGGAAGAAGT

TAATCCAGATATTGAATAA

＞KO108276 gi|9989061|gb|AAG10824.1|AC011808_12 Similar to molybdopterin cofactor

su|furase[Arabidopsis thaliana]

MEAFLKEFGDYYGYPDGPKNIQEIRDTEFKRLDKDYSCLFTSGATAALKLVGETFPWTQ

DSNFLYTMENH

NSVLGIREYALAQGASACAVDIEEAANQPGQLTNSGPSIKVKHRAVQMRNTSKLQKEES

RGNAYNLFAFP

SECNFSGLRFNLDLVKLMKENTETVLQGSPFSKSKRWMVLIDAAKGCATLPPDLSEYPA

DFVVLSFYKLC

KMVEFVWHLMNIILTGTVAASIADIDFVKRRERVEEFFEDGSASFLSIAAIRHGFKLLKSLT

PSAIWMHT

TSLSIYVKKKLQALRHGNGAAVCVLYGSENLELSSHKSGPTVTFNLKRPDGSWFGYLEV

EKLASLSGIQL

RAGHICWDDNDVINGKPTGAVRVSFGYMSTFEDAKKFIDFIISSFASPPKKTGNGTVVS

GRFPQLPSEDL

ESKESFPSHYLKSITVYPIKSCAGFSVIRWPLCRTGLLHDREWMVQGLTGEILTQKKVPE

MSLIKTFIDL

EEGLLSVESSRCEDKLHIRIKSDSYNPRNDEFDSHANILENRNEETRINRWFTNAIGRQC

KLLRYSSSTS

KDCLNRNKSPGLCRDLESNINFANEAQFLLISEESVADLNRRLEAKDEDYKRAHEKLNP

HRFRPNLVISG

GEPYGEDKWKTVKIGDNHFTGKILFGTLLRYEIDEKRQCWIGVGEEVNPDIE

At3g07575 MLP32，SEQ ID No.93

＞KO189051 (gi|12408710) arabidopsis thaliana chromosome III P1MLP3 genome sequence, full length sequence

ATGAAGCTTTATTCTGTTTCCATCATCATCTTCGTCTTAATTGCTCTCTCCACCATAG

TTAATGCTCAAC

AAGCTGCTACAGATTCCTGCAACTCAACTCTACCTCTCAACGACCTCACCTTCAACA

CCAGCCTCCTTCA

ATGCACCGAAGCTTGGACTCCCCAAAATTTCATCCTCCGATATGCAAGAACGGCAG

AGAACACATGGAGC

TTTATCTTATCGGCGCCGGATTCAAGCGCTTTCATCGGGATCGGATTCTCTACCAAC

GGTCAGATGATCG

GAAGCAGCGCGATCGTTGGTTGGATACCTTCCGACGGCGGTTCCGGGACTGTGAA

ACCGTACTTGCTCGG

TGGGAAATCTCCCGGAGAGGTTAATCCTGACCAAGGAGATCTAACGATCGTCAACG

GCTCGTTGAAGATC

GAATCAGTGTCGTCGCGTCTTTACATGAGATTTCAATTGACGGCGACGCTGCCGCG

GCAGAGTCTTCTTT

ACGCTGTGGGACCTGCCGGATTCTTCCCATCTTCGCCGGATTTTAGGTTGAGAGAG

CACCGCTTCGTGAC

CACCACGACCATCAATTATAATACAGGTTCGCAAAGTGTGGTTAAAGTTTCACCACA

CTCTAAGCTAAAG

AAGACACATGGGCTAATGAACATGTTCGGCTGGGGAATATTGATTATCGTTGGCGC

CATAGTGGCTCGAC

ATATGAAGCAATGGGACCCCACTTGGTTCTATGCCCATATCGCTCTCCAAACCACTG

GTTTTCTCCTCGG

TTTAACTGGTGTCATTTGCGGTTTGGTTCTTGAAAACCGGCTCAAGGCCAATAATGT

TTCCAAGCACAAA

GGCCTCGGGATAACCATACTTGTCATGGGCGTTCTTCAGATGCTGGCATTGCTAGC

TCGGCCGGATAAGC

AATCGAAATACAGAAAATATTGGAATTGGTATCATCATAACATAGGAAGACTTCTGAT

CATACTGGCTAT

TTCTAACATCTTCTACGGTATTCATTTGGCTAAAGCTGGAACTAGTTGGAATGGTGG

TTACGGTTTTGCG

GTCGCGGTCTTGGCCTTGACGGCTATTGGATTAGAAGTTAGAAAGTTCTTGAAAAAA

AATTGGAAGAAGA

AGAAGAAAGAGATGTTGAGAACTCGCCTTCTCTGGTTTACGCTTGGTTTTTCCGTGA

CCGGAGGTTCCAT

TGCTCATATCGTGTGGCGTGATCTCTATGCCGAACGTTTCGCTATTTCTTCTGATAT

GAAGGAGAAATTC

AGTGCTCTGGAAGGTAGAGTATCAGGTTTGGAGTCTGGTGGTTATGAGAACCCGAA

TCCAGCTCAGGTCA

GCTCTTTCTCTACCTCTCTCCCTCCATTCGTAACTATGATTTGA

＞KO189051 gi|6466940|gb|AAF130751|AC009176_2 agnoprotein [Arabidopis thaliana]

MKLYSVSIIIFVLIALSTIVNAQQAATDSCNSTLPLNDLTFNTSLLQCTEAWTPQNFILRYA

RTAENTWS

FILSAPDSSAFIGIGFSTNGQMIGSSAIVGWIPSDGGSGTVKPYLLGGKSPGEVNPDQGD

LTIVNGSLKI

ESVSSRLYMRFQLTATLPRQSLLYAVGPAGFFPSSPDFRLREHRFVTTTTINYNTGSQS

VVKVSPHSKLK

KTHGLMNMFGWGILIIVGAIVARHMKQWDPTWFYAHIALQTTGFLLGLTGVICGLVLENR

LKANNVSKHK

GLGITILVMGVLQMLALLARPDKQSKYRKYANWYHHNIGRLLIILAISNIFYGIHLAKAGTS

WNGGYGFA

VAVLALTAIGLEVRKFLKKNWKKKKKEMLRTRLLWFTLGFSVTGGSIAHIVWRDLYAERF

AISSDMKEKF

SALEGRVSGLESGGYENPNPAQVSSFSTSLPPFVTMI

The membranin that＞BN42839310 infers

atgaagatgaacctttattcttccgtttcttttatcttcttcaccttaatcgctcttcaatgtccacctctcaccattcagcaaactacg

gattcatgcagttcaactctaccgctcaacgacctcaccttcaactcaagcctccttcaatgcgtcgaagcatggactccaca

gaactacatccttcgatatgcaagaacgttagagaacacatggagcttcatcttatcggctccagactccaacgtcttcatcg

ggatcggattctccaccaacggtcagatgatcggatccagtgccgtggtcgggtggttacctcccggaagcggaggagga

ggacaggcgaaacaatactttctcggaggacagtctccgggagaagtaacgcctgaccaaggagacttagtgatcgtca

acggttctttaaagatcgagtcagtgtcgtcgcgtctttacatgagttttaagttgacggctgagctgccgcggcagagcattctt

tacgctaagggacctgccggattcttcccgtcttcgccggggtttaggttgagggagcaccaagccatgaccaccaccacc

atcaattataatacaggttcgcaaagtgtggttaagggttcaccacactctaagctaaggaagacacatgggctaatgaac

atgactggttggggaatactaatcatcattggcgccatagttgctcgacacatgaagcaatgggagccgacttggttctattct

catatcgctgtccagatcactggctttctcctaggcttaactggtatcatttgcggtttgattcttgaaaaccgaaccaacgctagt

aatgtttccacgcacaaagcccttgggataacaatactcgtcatgggtggtctccaggtactagcgttgcttgctcgaccgga

caaagaatcgaaatacaggaaatattggaactggtatcatcacaacataggaagagctttgataatactcgctatttctaac

atcttctatggtattcatttggctaaagctggctcttcttggaacgctggttacggttctgcggttggtgtcttggctttggctgctact

ggattagaagttagaaagctaatgaacaaatga

The membranin that＞BN42839310 infers

mkmnlyssvsfifftlialqcppltiqqttdscsstlplndltfnssllqcveawtpqnyilryartlentwsfilsapdsnvfiglgfstn

gqmigssavvgwlppgsggggqakqyflggqspgevtpdqgdlvivngslkiesvssrlymsfkltaelprqsilyakgpa

gffpsspgfrlrehqamttttinyntgsqsvvkgsphsklrkthglmnmtgwgiliiigaivarhmkqweptwfyshiavqitgfl

lgltgiicglilenrtnasnvsthkalgitilvngglqvlallarpdkeskyrkywnwyhhnigraliilaisnifygihlakagsswn

agygsavgvlalaatglevrklmnk*

At1g12800 F13K23.5 SEQ ID No.95

＞KO-T3-01-03305-1 F13K23.5

atggacgttctcgccttatcctcttccgcttccgccgccgcaccctccgcttctctcgccggaaaattcctgtcgtttccttctagg

gttagagtgagaagaaaccgagagaatttgttagctaaacagaagaagtttttagtttctgcttcgaaaagagaagagccta

agctcaacgaatgggatcaaatggagctcaactttggccgtttactcggcgaagacccgaaattgactttggctaagatagt

agctagaaaagtggatccagaagcttcttttattgacattgagaaatctttctacaagaacaaaggtaaaattcctgaagttga

agagattccattggattggtcaaaggataacaagaagaaatctactagttcactggatggattgaaattggtaaagcctgttct

gaaagatggagtcaagttcgaaaggccagtgatgaagaagccaagccctgttttgaagaagccattggtggaggctgttg

ctgctccaaaggtgcagagattgcctaatgttatattgagaaagccgagttcgttttatactagtaatggtgatgatgaggagtc

taagttgcggttgaaaccgaatctgacattgaaaatgagaaatgagagggaaaatgagaggtttagtgatatgacattgttg

agaaaaccggaaccagtgagcgtagttgcagaagaggaagacaagcctctttctgatgatttaactatggaggaaggag

aacaggaaggtggaacatattcacagtatactcttttggagaagccagaagcgaggctccagcctgtcaatgtagaagag

gaagttggagatagcggaggagtggaatcatctgagatagtaaacaactcaattcagaagccagaagcaaggccagag

cttgagaacatagaaaaggaagttgcagatagcggagttttggaatcatcggagatagaaaataattcaattccaactgaa

atgcagctcaatagcgagatgtcctctgaggagaaaactattaacagtgatccactcgagagaattccttcgaaaccaattt

ctcaaaccatcgtcgaagcttctttacaagggaaaccacaaagattagacccgtcttccgctgagccatcagttccgaacat

aggaaaaccgtcagtcgtgaaccatgaaggccgtcaggtctctgttgagctcaagggccctcctaccagatcgtccttgga

ggaaaatgattggaataaggcagagtctctagttaaaacagaattacgagcagatgttgagctaataagttcaagcactag

aggatttgctgtttcctatggatctttgattggatttttaccctaccggaaccttgcagcaaaatggaagtttctcgcatttgaatcat

ggttaagaagaaaaggtgtagatccatcaccgtatcgacaaaaccttggggtaattggaggtcaagatgtcacgagtaaat

ctccatctccagattcaagcttagattctgaagtcgctacaacgatcaacggagaagtttcttctgatatgaagctggaagatc

ttcttatggtatatgacagagagaagcagaagttcctgtcatcttttgttggtcagaaaatcaaagtgaatgttgttatggcaaat

cgaaattcaaggaagcttatattttcaatgaggccgagagaaaatgaagaggaagttgagaaaaaacgaactcttatggc

taagcttcgtgttggggatgttgtgaaatgctgcatcaagaaaattacctattttggtattttctgtgagctagaaggtgtccctgc

attggttcaccagtcagaagtttcatgggatgcaactttagaccctgcttcatatttcaagattggtcagattgtggaagcgaaa

gtgcaccagctagattttgctcttgaacgtatctttcttgtcattaaaagaaattacgcctgatcctcttactgaagctttagaatctg

tagttggtggtgataatgatcagttggggggacgattacaagcagcagagctcgacgctgaggtttctgaaacctttcttctgc

agtggcctgacgtggaatctctgatcaaagagctggaaatggttgaaggaatccaatcagtctcaaaaagtcgtttcttcttg

agtccgggtcttgctccaacgtttcaggtttacatggctccaatgtttgagaaccaatacaaactgcttgctcgagctggaaac

agagtacaagagcttattgttgaagcatccttgagcaaagaagagatgaaatctacaatcatgtcttgcaccaacagagta

gaatga

The S1 albumen of＞KO03305 gi|8698727|gb|AAF78485.1|AC012187_5 and people gb|U27517

The tool similarity also contains S1 RNA in conjunction with the PF|00575 structural domain, EST gb|F15428.5|

From this gene.[Arabidopis thaliana]

MDVLALSSSASAAAPSASLAGKFLSFPSRVRVRRNRENLLAKQKKFLVSASKREEPKLN

EWDQMELNFGR

LLGEDPKLTLAKIVARKVDPEASFIDIEKSFYKNKGKIPEVEEIPLDWSKDNKKKSTSSLD

GLKLVKPVL

KDGVKFERPVMKKPSPVLKKPLVEAVAAPKVQRLPNVILRKPSSFYTSNGDDEESKLRL

KPNLTLKMRNE

RENERFSDMTLLRKPEPVSVVAEEEDKPLSDDLTMEEGEQEGGTYSQYTLLEKPEARL

QPVNVEEEVGDS

GGVESSEIVNNSIQKPEARPELENIEKEVADSGVLESSEIENNSIPTEMQLNSEMSSEEK

TINSDPLERI

PSKPISQTIVEASLQGKPQRLDPSSAEPSVPNIGKPSVVNHEGRQVSVELKGPPTRSSL

EENDWNKAESL

VKTELRADVELISSSTRGFAVSYGSLIGFLPYRNLAAKWKFLAFESWLRRKGVDPSPYR

QNLGVIGGQDV

TSKSPSPDSSLDSEVATTINGEVSSDMKLEDLLMVYDREKQKFLSSFVGQKIKVNVVMA

NRNSRKLIFSM

RPRENEEEVEKKRTLMAKLRVGDVVKCCIKKITYFGIFCELEGVPALVHQSEVSWDATL

DPASYFKIGQI

VEAKVHQLDFALERIFLSLKEITPDPLTEALESVVGGDNDQLGGRLQAAELDAEVSETFL

LQWPDVESLI

KELEMVEGIQSVSKSRFFLSPGLAPTFQVYMAPMFENQYKLLARAGNRVQELIVEASLS

KEEMKSTIMSC

TNRVE

At5g23080 MYJ24.7，SEQ ID No.97

＞KO146082 (gi|2351073) arabidopsis thaliana genomic dna, karyomit(e) 5, P1 clone: MYJ24

ATGGGGTCAGACGAGGAAGATTTCGTGTTTCATGGAACGCCAATAGAGCGCGAAGA

AGAAATCGCAAGCC

GGAAGAAGAAAGCAGTCGCTGGGGCTTCTGGCAATCTTAGAACTCTCCCTGCTTGG

AAGCAAGAGGTGAC

TGATGAAGAAGGCCGTAGAAGGTTCCATGGAGCATTTACTGGTGGATATTCTGCTG

GGTATTACAATACA

GTTGGATCAAAAGAGGGCTGGGCTCCACAGTCATTTACATCATCAAGGCAGAACAG

AGCTGGAGCGAGAA

AGCAAAGTATTTCAGACTTTCTAGATGAAGATGAAAGGCGGATATGGAGGGCAAAT

CACTGTCTGCGAG

CTCACAATTTGACACATTTGGGTTTACGGCAGCCGAACATTCCCGCAAGCATGCTG

AGAAAGAACAGCAT

GAGAGGCCATCAGCCATTCCTGGCCCTGTTCCTGACGAACTTGTTGCTCCAGTTTC

AGAGTCAATTGGGG

TCAAACTTTTGCTAAAGATGGGATGGCGGCGTGGTCATTCAATAAAGGAAGTGCGT

GCCAGTTCAGATGC

TCGTAGAGAAGCTAGAAAAGCATTCTTAGCCTTCTATACTGATGAGAATACAAAGGA

AACGCCCGACTCG

CTTGTTTCTGAGACTGAAGTGGAAACTTCTCTGGGTGAAGATATTAAAATTTCTGAAA

GCACTCCTGTAT

ATGTTCTGAATCCAAAGCAAGATCTGCATGGATTAGGATATGATCCTTTTAAGCATG

CTCCTGAATTTAG

AGGAAAGATTGCTCCGGGTTTTGGCATTGGAGCACTTGAGGAACTTGATGTTGAGG

ATGAAGATGTCTAT

GCTGGTTACGATTTTGATCAGACTTATGTCATAGAAGACGAACAGCCAGCAAGACA

GAGCAATGACAATA

GACTGAGGTTAACCTCAAAAGAGCATGACGTTCTGCCAGGTTTTGGAGCTGCTAAG

AATTCTGACTACAG

TATGGAGAGATTTAATCCTCCGATAATCCCGAAGGATTTTGTGGCCCGGCATAAATT

TTCTGGTCCTCTT

GAGGCTGAAACTAAGCCAACTGTTTCTGCTCCTCCGGAAGTTCCTCCTCCTGCAGA

TAATAATCTGAAAC

TTCTGATCGAGGGGTTTGCAACTTTTGTTTCCCGTTGCGGGAAACTATACGAGGATC

TTTCTAGAGAGAA

GAACCAATCAAATCAGCTGTTTGATTTTCTTCGGGAAGGTAACGGTCATGACTACTA

CGCAAGAAGGCTG

TGGGAGGAGCAGCAAAAGCGTAAAGATCAAAGTAAGCTGACATTAGATGTTAAGGT

GTCTCCAACCGTAC

AGAAAATGACTGCAGAAACACGTGGCAGCTTATTAGGGGAAAAGCCATTGCAGAGA

AGTTTGAAAGAAAC

CGATACTTCTGCTTCTTCTGGAGGCTCCTTCCAGTTCCCGACCAATCTCTCTGACAC

ATTCACCAAATCA

GCTTCATCTCAAGAGGCAGCAGATGCTGTGAAGCCCTTCAAAGATGATCCAGCTAA

ACAAGAAAGATTTG

AGCAGTTTCTCAAGGAGAAATACAAAGGAGGGTTACGTACAACAGACTCCAACAGA

GTTAATAGCATGTC

GGAATCAGCTCGGGCACAAGAGAGGCTGGACTTTGAGGCTGCAGCCGAGGCAATT

GAGAAAGGGAAAGCT

TACAAGGAGGTCAGACGGGCTACCGAACAGCCTCTCGATTTCCTTGCTGGAGGTCT

TCAGTTTACTTCTG

GGGGAACAGAGCAAATTAAAGACACTGGAGTGGTAGACATGAAATCGAGTAAGACG

TATCCTAAAAGGGA

AGAGTTCCAATGGCGTCCTTCACCTCTTTTGTGCAAACGTTTTGATCTCCCCGATCC

ATTCATGGGAAAG

CTGCCACCTGCTCCGCGAGCGAGAAACAAAATGGATTCTCTCGTATTCTTGCCGGA

TACAGTGAAAGCTG

CATCTGCACGTCAAGTATCTGAGTCGCAAGTACCTAAGAAAGAGACATCAATAGAAG

AGCCTGAAGTTGA

GGTAGAAGTGGAGAATGTGGAGAGACCTGTTGATCTTTACAAGGCTATCTTCTCTGA

TGATTCTGAAGAT

GATGAAGATCAACCAATGAATGGAAAGATACAAGAGGGTCAAGAAAAGAAGAATGA

AGCGGCTGCAACCA

CATTAAACCGGCTTATAGCTGGCGATTTCCTAGAATCTTTAGGGAAAGAACTAGGGT

TCGAGGTGCCAAT

GGAAGAAGAGATCAAGTCCAGAAGCAAACCCGAAGATTCTTCTGATAAAAGACTTG

ATCGACCCGGATTG

AAAGAGAAAGTGGAGGAGAAGACAAGCAGCCTCACACTTGGGTCTGAAGAAGAAAA

GAGTAGAAAAAAGA

GAGAGAAATCGCCAGGAAAACGGAGTGGTGGCAACGATCTATCATCGAGTGAATCC

TCAGGAGATGAACG

GAGGAGAAAACGATATAATAAGAAGGATAGACATAGAAACGATTCAGAGAGCGATT

CATCCAGCGACTAC

CACAGCAGGGATAAGCAAGGATCAAGATCTAGGAGCAAGCGGAGAGAATCTTCTAG

AGAGAAGAGAAGTA

GCCACAAGAAGCACTCAAAGCATCGCAGGACCAAGAAGTCTTCTTCTTCACGGTAT

AGCTCAGACGAAGA

ACAAAAAGAGTCAAGGCGGGAGAAGAAGAGGCGACGAGACTGA

＞KO146082 gi|9759366|dbj|BAB09825.1|gene_id:MYJ24.7～agnoprotein [Arabidopis thaliana]

MGSDEEDFVFHGTPIEREEEIASRKKKAVAGASGNLRTLPAWKQEVTDEEGRRRFHGA

FTGGYSAGYYNT

VGSKEGWAPQSFTSSRQNRAGARKQSISDFLDEDEKADMEGKSLSASSQFDTFGFTA

AEHSRKHAEKEQH

ERPSAIPGPVPDELVAPVSESIGVKLLLKMGWRRGHSIKEVRASSDARREARKAFLAFY

TDENTKETPDS

LVSETEVETSLGEDIKISESTPVYVLNPKQDLHGLGYDPFKHAPEFRGKIAPGFGIGALEE

LDVEDEDVY

AGYDFDQTYVIEDEQPARQSNDNRLRLTSKEHDVLPGFGAAKNSDYSMERFNPPIIPKD

FVARHKFSGPL

EAETKPTVSAPPEVPPPADNNLKLLIEGFATFVSRCGKLYEDLSREKNQSNQLFDFLRE

GNGHDYYARRL

WEEQQKRKDQSKLTLDVKVSPTVQKMTAETRGSLLGEKPLQRSLKETDTSASSGGSF

QFPTNLSDTFTKS

ASSQEAADAVKPFKDDPAKQERFEQFLKEKYKGGLRTTDSNRVNSMSESARAQERLD

FEAAAEAIEKGKA

YKEVRRATEQPLDFLAGGLQFTSGGTEQIKDTGVVDMKSSKTYPKREEFQWRPSPLLC

KRFDLPDPFMGK

LPPAPRARNKMDSLVFLPDTVKAASARQVSESQVPKKETSIEEPEVEVEVENVERPVDL

YKAIFSDDSED

DEDQPMNGKIQEGQEKKNEAAATTLNRLIAGDFLESLGKELGFEVPMEEEIKSRSKPED

SSDKRLDRPGL

KEKVEEKTSSLTLGSEEEKSRKKREKSPGKRSGGNDLSSSESSGDERRRKRYNKKDR

HRNDSESDSSSDY

HSRDKQGSRSRSKRRESSREKRSSHKKHSKHRRTKKSSSSRYSSDEEQKESRREKKR

RRD

At5g38680 MBB18.23 SEQ ID No.99

＞KO109111 (gi|8099974) arabidopsis thaliana genomic dna, karyomit(e) 5, P1 clone: MBB18

：MBB18

ATGTCGTCCCCGGAAAAGTTTTCGCCAGCGCCGGAATCGAACTCAAATCCGTCACT

TCCCGATGCTTTGA

TAATAAGCTGCATCGCACGAGTCTCAAGATTGTATTATCCGATTCTCTCCTTTGTCTC

CAAGAGCTTTCG

ATCTCTCCTAGCTTCACCGGAGCTTTACAAGGAACGGTCACTCTTGAACCGCACCG

AGGGTTGTCTATAT

GTATGCTTATACTTAAATCCTTTTGAGAGCCCTAGCTGGTTTACTCTCTGCTTGAAAC

CTGATCAAGCCC

TATCTTCTGAAACAAGTAATAAGAAGAAGTCAAGTGGGTATGTTTTGGCTACAGTAT

CAATTCCACATCC

TCGTCTTGTGCAACGTTCCAGTCTCGTGGCGGTTGGTTCTAATATCTACAACATTGG

CAGATCCATATCA

CCTTACTCTAGTGTCTCGATTTTTGATTGCCGGTCTCACACGTGGCGCGAGGCTCC

AAGCTTGCCAGTGG

AGCTAGTTGAAGTTTCTGCTGGCGTCCTTGACGGAAAGATATATGTAGCCGGAAGT

TGCAAAGATGGAGA

TTCTCTTAACTTGAAGAACACTTTCGAGGTGTTCGACACAAAAACACAAGTTTGGGA

TCATGTACCTATC

CCTTACAACGAAACAAAACACAACATTTACTCCAAAAGCTTATGTATTGACGAAAAGT

GGTATGTAGGGG

CTAAGAGAAAGGTGGTTTCTTACAATCCCAAGAAAGGTATATGGGACCTTGTTGAAT

CAGAGATGTGTAG

TTATAAGTCTTCATATGATTATTGTGAGATAGAGAACGTTTTGTACTCTGTCGAAAAA

ACATGGCGTGGC

ACTGTTTTCAGATGGTATGACACTGAGCTAGGACGGTGGAGAAAGTTGGAGGGTTT

GAATATGCCTTATA

GTGGGACTGGTGACAGAGGCGGTAAGAAGATGATTTGGTGTGCGGTGATTACGCTT

GAAAGGCGCAAAAA

TAGTGGAATTTGGGGAAACGTTGAGTGGTTTGCTCATGTGCTTACAGTTCCTAAAAC

ATTTGTTTTCCAA

AAGTTTCTTGCTGCTACTGTCTAA

＞KO109111 gi|10176836|dbj|BAB10158.1|gene_id：MBB18.23～pir||T09563～

Similar agnoprotein [Arabidopis thaliana]

MSSPEKFSPAPESNSNPSLPDALIISCIARVSRLYYPILSFVSKSFRSLLASPELYKERSLL

NRTEGCLY

VCLYLNPFESPSWFTLCLKPDQALSSETSNKKKSSGYVLATVSIPHPRLVQRSSLVAVG

SNIYNIGRSIS

PYSSVSIFDCRSHTWREAPSLPVELVEVSAGVLDGKIYVAGSCKDGDSLNLKNTFEVFD

TKTQVWDHVPI

PYNETKHNIYSKSLCIDEKWYVGAKRKVVSYNPKKGIWDLVESEMCSYKSSYDYCEIEN

VLYSVEKTWRG

TVFRWYDTELGRWRKLEGLNMPYSGTGDRGGKKMIWCAVITLERRKNSGIWGNVEWF

AHVLTVPKTFVFQ

KFLAATV

At2g28470 SEQ ID No.101

＞KO-T3-02-23318-1 At2g28470

atggttaaagtaaggaagatggagatgattttattactaattcttgtgattgtggtggcggcgacggcggcgaatgtgacttatg

accaccgtgcattagtaatcgacgggaaacggaaagttctaatctctggttctattcattatcctcggagtactcctgagatgtg

gccagagcttatacagaaatctaaagacggtggtttagatgttatagagacgtatgtgttttggagtggtcacgaaccggaga

aaaataagtataattttgaaggaagatatgatttagtgaaatttgtgaagcttgcggctaaagctggtctctatgttcatttaaga

attggtccttacgtctgtgctgaatggaattacggtggtttcccagtgtggttgcattttgttccaggaattaagtttcgaactgata

atgagccatttaaggaagaaatgcagagatttaccacaaagattgttgatttgatgaagcaagaaaagctttatgcatcaca

aggaggtccaatcattctctcgcagattgagaatgaatatggaaatattgactcagcttatggtgcggctgctaaaagttatat

caagtggtctgcttctatggctctttcgttagatactggagtaccatggaatatgtgtcaacaaacagatgctcctgatcccatg

atcaacacatgcaatggtttctactgtgaccagtttacacctaactcaaataataaaccaaagatgtggaccgagaactgga

gtggatggttccttggttttggagatccttctccttacagaccagttgaagatcttgcatttgcggtcgcgcggttttaccaacgag

gtggaacgttccagaactattacatgtatcacggtggaacaaactttgatagaacaagtggaggaccattaatctctactagt

tatgattatgatgctccaattgatgagtatggactacttagacaaccaaaatggggacacttacgagatctacacaaggctat

caagctttgtgaagatgcattgattgccacagatccaacaattacttctctaggttcaaatttggaggctgctgtatataaaaca

gaatctggatcatgtgctgcttttcttgcaaatgttgacacgaagtctgatgcactgtgactttaatggaaaatcatataactt

gcctgcatggtccgtaagcatcttgccggattgcaaaaatgtagctttcaataccgcaaaggtaaagttcaatagcatctcta

aaactcccgatggtggttcgtctgcggagttaggttcacaatggagttacattaaagaacctattggaatttccaaagctgatg

cattcttgaaacctggattgctagagcagattaacacaacagctgataaaagcgattacttgtggtactcactaaggacggat

ataaaaggcgatgagactttccttgacgagggatctaaagccgtccttcacattgaatctcttggtcaagtggtctatgcttttat

aaatggaaaacttgcaggaagcggacatggcaaacagaagatttctttggatataccgattaatctttgtaaccgggacgaa

cacaatcgatctccttagtgttaccgtagggcttgcgaattatggagctttctttgacttagtgggagcaggaataaccggacct

gtgacacttaaaagcgctaaaggtggtagctcaattgatttggcttcacagcaatggacttatcaggttggactcaaaggag

aagacacaggtttggcaactgtagattcttctgaatgggtttcaaagtctcctttgcctactaaacaaccacttatttggtacaag

acgacatttgatgctccttctgggagcgagccagtagctatagacttcacgggtacaggaaagggtattgcatgggtgaatg

gacagagcataggtaggtactggccaactagtatcgctggaaatggcggttgtacagaatcatgcgactatagaggttctta

ccgtgcaaacaaatgcctcaagaactgtggaaaaccttcacagacattgtatcatgtacctcgctcgtggctaaaaccgag

cgggaacatacttgttctgtttgaggagatgggaggagatccaacacaaatatcatttgcgacaaaacaaacaggaagca

atctttgtctaacggtgtcacagtctcatccaccaccggtggacacatggacttccgactcaaagatctcaaacagaaacag

aaccaggccggttctttcgttgaaatgccctatctctactcaggtgatattttctataaaatttgcaagctttggtacacccaaag

gtacttgcggtagcttcacacaaggccattgcaatagctctcgatctctctccctcgtccaaaaggcatgtattggattgagga

gttgcaacgttgaagtatcgactagagtgttcggggaaccttgtcgtggcgtcgtcaagagcttagctgttgaagcttcttgttca

tga

The beta-galactosidase enzymes that＞KO23318 gi|4510395|gb|AAD21482.1| infers [Arabidopis thaliana]

MVKVRKMEMILLLILVIVVAATAANVTYDHRALVIDGKRKVLISGSIHYPRSTPEMWPELI

QKSKDGGLD

VIETYVFWSGHEPEKNKYNFEGRYDLVKFVKLAAKAGLYVHLRIGPYVCAEWNYGGFP

VWLHFVPGIKFR

TDNEPFKEEMQRFTTKIVDLMKQEKLYASQGGPIILSQIENEYGNIDSAYGAAAKSYIKW

SASMALSLDT

GVPWNMCQQTDAPDPMINTCNGFYCDQFTPNSNNKPKMWTENWSGWFLGFGDPSP

YRPVEDLAFAVARFY

QRGGTFQNYYMYHGGTNFDRTSGGPLISTSYDYDAPIDEYGLLRQPKWGHLRDLHKAI

KLCEDALIATDP

TITSLGSNLEAAVYKTESGSCAAFLANVDTKSDATVTFNGKSYNLPAWSVSILPDCKNVA

FNTAKVKFNS

ISKTPDGGSSAELGSQWSYIKEPIGISKADAFLKPGLLEQINTTADKSDYLWYSLRTDIKG

DETFLDEGS

KAVLHIESLGQVVYAFINGKLAGSGHGKQKISLDIPINLVTGTNTIDLLSVTVGLANYGAFF

DLVGAGIT

GPVTLKSAKGGSSIDLASQQWTYQVGLKGEDTGLATVDSSEWVSKSPLPTKQPLIWYK

TTFDAPSGSEPV

AIDFTGTGKGIAWVNGQSIGRYWPTSIAGNGGCTESCDYRGSYRANKCLKNCGKPSQT

LYHVPRSWLKPS

GNILVLFEEMGGDPTQISFATKQTGSNLCLTVSQSHPPPVDTWTSDSKISNRNRTRPVL

SLKCPISTQVI

FSIKFASFGTPKGTCGSFTQGHCNSSRSLSLVQKACIGLRSCNVEVSTRVFGEPCRGVV

KSLAVEASCS

＞GM59789916 beta-galactosidase enzymes

atgagaacatcacaaattctgttggttttgctttggttcttctgcatttatgccccttcttcgtttggagcaaatgtcacgtatgacca

cagagcattggtcattgatggcaagcgccgagtcttggtatctggttctattcattaccctcgtagcactccagagatgtggcca

gacctcattcagaaatccaaagatggaggacttgatgtgattgagacttatgttttttggaacttacacgaaccagttagaggc

cagtataactttgaaggtaggggcgatttggtcaaatttgtgaaggtagtagcagcagcaggtctatatgtgcatctccggatt

ggtccatacgcatgtgctgaatggaactacggtggtttccctctttggctacattttattccgggaattcagttccgaactgataa

caaaccatttgaggcagaaatgaagcagttcaccgctaagattgtggatttgatgaagcaagagaacctctatgcatcaca

gggaggacctattattttgtctcagattgaaaatgagtatgggaacattgaagcggattatggtcctgctgctaaatcctacatc

aaatgggcagcatcaatggcaacatctcttggtacaggggttccttgggtaatgtgccaacagcaaaatgctcctgatccaa

ttattaacgcgtgcaatggattttactgcgatcaattcaaaccaaactctaacacaaaaccaaaaatatggactgagggttat

accggatggtttcttgcatttggtgatgctgtgcctcacagaccagtggaagatcttgcatttgctgtggcacgcttttaccagcg

aggtggaacttttcaaaattactatatgtaccatggagggactaattttggccgggcttctgggggaccttttgttgctagtagtta

tgattatgatgcaccaattgatgagtatggatttattagacagcctaagtggggccaccttaaagacgtgcataaggccataa

aactttgtgaagaagcactgatagctactgatccaacaattacatctcttggaccaaatatagaggctgcagtttacaagaca

ggagttgtatgtgctgccttccttgctaacattgccacatctgatgcaacagtgaccttcaatggaaattcatatcacttgcccgc

atggtctgtgagcatcttaccagactgcaagaatgtagtacttaatactgcaaagattacttctgcatctatgatttcaagcttca

caactgaatctttaaaagatgttggttctttggatgattctggctcaagatggagttggattagtgaacctatcggtatttcaaagg

ctgattcattctcaacatttggattgctggagcaaataaatacaactgctgatagaagtgattacttgtggtactcattaagcatt

gatcttgatgctggtgctcaaactttccttcatattaaatcccttgggcatgctcttcatgctttcataaatgggaagcttgcaggg

agtggaaccggcaaccatgagaaagctaatgtcgaagtagacatccccatcacactagtttctgggaagaacacaattga

tctcctgagtttaactgtgggacttcagaactatggagctttttttgacacatggggtgcggggatcactggccctgtgatattga

aatgtttgaagaatggcagcaatgttgatctctcctccaagcagtggacatatcaggttggccttaaaaatgaagatttaggtc

tatctagtggctgttctggacagtggaattcacaatctaccttacctacaaatcaaccgttgacttggtacaagacaaacttcgt

tgcaccctccggtaacaacccagttgcaattgacttcacggggatgggaaaaggtgaggcttgggtgaatggacagagca

ttgggcgatactggcctacatatgcctctccaaaaggtggttgtactgattcatgcaattatagaggagcctatgatgcatcca

aatgtctcaagaactgtggaaaaccatcacagacattataccatgtacctcgatcatggttacgaccagatagaaacacac

ttgtattgtttgaggaaagtggaggcaaccctaagcaaatctcttttgccacaaaacaaataggaagcgtgtgttcacatgtat

ctgaatctcaccctccacctgtagactcgtggaattcaaatacagaatcaggaagaaaagtagttcctgtagtttcactggag

tgcccttatcctaatcaggtggtctcatccattaaatttgcaagttttggaacgcctcttgggacttgcgggaacttcaagcatgg

actctgcagcagcaataaggctctatccattgtgcagaaggcttgcattggatcaagcagttgtagaattgaactatcagttaa

tacattcggagatccatgtaaaggagtagcaaagagtttagctgttgaagcttcttgtgcatag

＞GM59789916 beta-galactosidase enzymes

mrtsqlllvllwffciyapssfganvtydhralvidgkrrvlvsgsihyprstpemwpdliqkskdggldvietyvfwnlhepvrg

qynfegrgdlvkfvkvvaaaglyvhlrigpyacaewnyggfplwlhfipgiqfrtdnkpfeaemkqftakivdlmkqenlyas

qggplllsqleneygnleadygpaaksyikwaasmatslgtgvpwvmcqqqnapdpiinacngfycdqfkpnsntkpki

wtegytgwflafgdavphrpvedlafavarfyqrggtfqnyymyhggtnfgrasggpfvassydydapideygfirqpkwg

hlkdvhkaiklceeallatdptltslgpnleaavyktgvvcaaflaniatsdatvtfngnsyhlpawsvsilpdcknvvintakits

asmissftteslkdvgslddsgsrwswisepigiskadsfstfglleqinttadrsdylwyslsidldagaqtflhikslghalhafi

ngklagsgtgnhekanvevdlpltlvsgkntldllsltvglqnygaffdtwgagitgpvillclkngsnvdlsskqwtyqvglkne

dlglssgcsgqwnsqstlptnqpltwyktnfvapsgnnpvaidftgmgkgeawvngqsigrywptyaspkggctdscnyr

gaydaskclkncggkpsqtlyhvprswlrpdmtlvlfeesggnpkqisfatkqigsvcshvseshpppvdswnsntesgrkv

vpvvslecpypnqvvssikfasfgtplgtcgnfkhglcssnkalsivqkacigssscrielsvntfgdpckgvakslaveasca

At3g11210 F9F8.1，SEQ ID No.103

＞KO153132 (gi|12408720) arabidopsis thaliana chromosome IIIBAC F9F8 genome sequence, complete sequence

ATGGTTGGACCCGCGCGGCCTCAGATCGTTTTGTTTGGATCTTCCATTGTTCAGATG

AGCTTTGGCCATG

GTGGTTGGGGCGCCATTCTTTCCGAGGTCTACGCTCGTAAGGCCGACATCATTCTG

CGAGGATATTATGG

ATGGAACTCTTCTCGTGCTTTGGAAGTTGTCGACCAAGTGTTCCCCAAGGATGCTG

CAGTACAACCTTCT

CTGGTCATTGTCTATTTTGGAGGAAACGACTCAATGGCGCCTCACTCGTCTGGACTA

GGACCTCATGTAC

CACTTACTGAATATGTTGATAACATGAAGAAGATCGCTCTTCATCTTCAGAGCCTTTC

AGACTTCACCCG

AATCATATTTCTTAGTTCTCCTCCAGTGGATGAGGCTAAAGTTCGCCAGAACCAAAG

CCCATACTTGAGC

GAGGTAATCCGCACAAACGACCTCTGCAAGACTTATTCAGATGCTTGTGTAGAGCT

GTGCCAAGAACTCG

GCCTAGAAGTAGTTGATCTCTTCTCTACTTTTCAGAAAGCAGATGACTGGAAAACTG

TTTGCTTCACAGA

CGGGATTCATTTGTCAGCACAAGGAAGCAAAATAGTAGCGGGAGAGATACTAAGAG

TGGTTAAAGAAGCG

GAATGGCATCCATCACTTCACTGGAAATCAATGCCAACAGAATTCGCAGATGACTCT

CCTTATGATCTTG

TATCAGCAGATGGCAAACAGACAGTAAATTCTTCAGAATGGACTTATTTCTGGGAAG

AACAATGGGACTA

A

＞KO153132 gi|6016678|gb|AAF01505.1|AC00999_1 agnoprotein [Arabidopis thaliana]

MVGPARPQIVLFGSSIVQMSFGHGGWGAILSEVYARKADIILRGYYGWNSSRALEVVDQ

VFPKDAAVQPS

LVIVYFGGNDSMAPHSSGLGPHVPLTEYVDNMKKIALHLQSLSDFTRIIFLSSPPVDEAK

VRQNQSPYLS

EVIRTNDLCKTYSDACVELCQELGLEVVDLFSTFQKADDWKTVCFTDGIHLSAQGSKIVA

GEILRVVKEA

EWHPSLHWKSMPTEFADDSPYDLVSADGKQTVNSSEWTYFWEEQWD

＞BN45447107 CPRD49

atggttggaccgtcgcggcctcagatcgttctttttggatcatccatcgtccagatgagctttggtcatggtggttggggtgctatt

ctctccgaggtctatgctcgcaaggccgacatcattctgcgaggatattatggatggaactcaactcgtgctttggaggttgttg

acaaagtgttccccaaggatgccgttgtacaaccttctcttgtagtcgtctattttggaggaaacgactcaatgggacctcatcc

ttctggtctaggacctcacgtgccactaactcaatacgttgataacatgaagaagatcgctcttcatcttcagagtctttcagact

caactcgtatcatatttctaagttgccctccagtggacgaagccaaagttcgtcaaaaccagagcccatacttgagcgaggt

aatccgcacaaacgagctatgcaagacatattcagacgcttgtgtagagctatgcaaagagctcgacttacaagtagtgga

tctcttctctactcttcagaaagcagatgactgggaaaccgtttgcttcacagatgggattcatttgtcagcacaaggaagcaa

gctggtggccgcagagatactgagagttgttaaggaagcggagtggagaccgtctcttcactggaaatcgatgccaacag

aattctcagaggactctccttatgatcttgttgcagcagatggcaaaacgacgttgaactcttcggagtggacgtacttctggg

aagaacaatgggagtaa

＞BN45447107 CPRD49

mvgpsrpqivlfgssivqmsfghggwgailsevyarkadiilrgyygwnstralevvdkvfpkdavvqpslvvvyfggnds

mgphpsglgphvpltqyvdnmkkialhlqslsdstriiflscppvdeakvrqnqspylsevirtnelcktysdacvelckeldlq

vvdlfstlqkaddwetvcftdgihlsaqgsklvaaeilrvvkeaewrpslhwksmptefsedspydlvaadgkttlnssewty

fweeqwe*

＞GM48908722 CPRD49

atggtgggaccagtgaggcctcagtttgtgctctttggctcttccattgttcagctcagtttttctctccaaggttggggtgctattctt

gctcacttgtatgctcgcaaggccgatataattctgcgaggatactctggttggaattcaaggcgtgctgtgcaagttctggatg

aaattttcccaaagaatgccactgagcaaccagaattgataattgtgtactttggtggtaatgattctcttcttccgcatccaagt

ggccttggtcaacatgtacctctgcaagaatacattgaaaatatgagaaagattgctatccatctgaagagcctttcaaagaa

gactcgccttatatttctcggtgctcctcctgtcaatgaggcacaaatttatggaaccagtgtgctacaagggcagcgattaag

gaacaatgaatcttgtcgaatatattcagaagcatgtttggagctgtgccgtgagatgaacatcatggcaattgatctgtggtct

gcactccagaaaagggttgactggagagaagtttgcttcacggatggaattcatcttacttctgaggggagcaatatagtgg

caaaagaggtattgaaggtcatcaaagaagcaaactgggaaccttgcctgcactggaggtcaatgccaactgaatatgg

agaagattcaccttatgatcctgttggccctgatggaaagacaagtttaaatatctccaactggaccttccttgaaaccaagg

aatgggactag

＞GM48908722 CPRD49

mvgpvrpqfvlfgssivqlsfslqgwgailahlyarkadiilrgysgwnsrravqvldeifpknateqpeliivyfggndsllphps

glgqhvplqeyienmrkiaihlkslskktrliflgappvneaqiygtsvlqgqrlmnescriyseaclelcremnimaidlwsal

qkrvdwrevcftdgihltsegsnivakevlkvikeanwepclhwrsmpteygedspydpvgpdgktslnisnwtfletkew

d*

＞GM51641000 CPRD49

atggctggcccaattatgagacctcagattgtgctatttggctcctccataattcaaatgagcttcgacaatggtggttggggtg

ctattctagctaacttgtacgctaggaaggcggacatcatcttaagaggatactctggttggaattcaaggcgggctttggag

gttttggatgaaattttccccaaggatgcttatgtgcaaccatcattggtaattgtgtattttggtggcaatgattctattgatcctca

cccatctggccttggtcctcatgtaccccttgaagaatatgttgaaaacatgaggaaaattgctaatcatcttaagagcctctcg

gaccatattcgcattatatttctcacttctcctccgatcaatgaagaactaatccgcaaaaagctcagtgcaacgcaatcagg

aagaaccaatgaatcctgtggagagtatgcagatgggttaatggagctttgtgaggagatgaatatcaaggccattaatctg

tggtctgcaattcagacaagagaggattggttagacgttagcttcacggatggagttcatctatcagcagagggaagcaag

gtagtggtgaaggaaatattaaaggttctaagagaagtagattggaaacctagtctgcattggatgtcaatgccaactgaat

atgcagaagattcaccatattatcctccaagtcctgatggaacaacaaccataaatgtgtctcatattatctcccgaaggtgttt

gcagtgggatatatag

＞GM51641000 CPRD49

magpimrpqivlfgssiiqmsfdnggwgailanlyarkadiilrgysgwnsrralevldeifpkdayvqpslvivyfggndsid

phpsglgphvpleeyvenmrkianhlkslsdhiriifltsppineelirkklsatqsgrtnescgeyadglmelceemnikainl

wsaiqtredwldvsftdgvhlsaegskvvvkellkvlrevdwkpslhwmsmpteyaedspyyppspdgtttinvshiisrrcl

qwdi*

＞GM51230662 CPRD49

atgccaggatcattgaggcctcggtttgttatctttggttcttccatcgttcaatttggtttttatgatgaaggttgggtggctattctttc

tcatttgtatgcccgcaaggttgatattgatttgcgaggatatgctggttggaattcaaggcgtgctgtgcaggttctggataaag

tttttcccaaggatgcccctatacaaccttcattggttattgtctactttggtggtaatgattcttctgctcccctctcatctggcctag

gtcctcatgtgcctctccaagaatacattgaaaatttgaggaagatcgttgaccatctcaagagcctctcagagaacactcgc

attctacttctcagtactcctcccctcaatgatgcagcaattacgccaaacagtgatgggaagccaacaaagacatatgaag

cttgtcaaatatattcagaagcatgtttggatgtgtgccgcaagatgaatatcaaggccattgatttgtggtctgctattcagaaa

agagataactggcaagatgtttgcttcattgatggaattcacctctcatctgagggaagcaagatagtgttgaaagagatact

gaatgtcctcaaaggtgcagaatgggaacctagtctatattggaaatcaatgccaagtgagtttgatgaagattcaccatatg

atccagttacaactgatggaaagtcaactattaatctttccagctgggtcttccctgacaatgacaaatgggactag

＞GM51230662 CPRD49

mpgslrprfvlfgsslvqfgfydegwvailshlyarkvdidlrgyagwnsrravqvldkvfpkdapiqpslvivyfggndssapl

ssglgphvplqeyienlrkivdhlkslsentrilllstpplndaaitpnsdgkptktyeacqiyseacldvcrkmnikaldlwsalq

krdnwqdvcfldgihlssegskivkeilnvlkgaewepslywksmpsefdedspydpvttdgkstinlsswvfpdndkwd

*

At5g03730 F17C15_150，SEQ ID No.105

＞KO175352 (gi|7340643) Arabidopis thaliana DNA karyomit(e) 5, BAC clones F17C15 (ESSA plan)

ATGGAAATGCCCGGTAGAAGATCTAATTACATTTGCTTAGTCAATTTTCTGACGAT

CAGGTGTCAGTTT

CCGTCACCGGAGCTCCTCCGCCTCACTATGATTCCTTGTCGAGCGAAAACAGGAGC

AACCATAACAGCGG

GAACACCGGGAAAGCTAAGGCGGAGAGAGGCGGATTTGATTGGGATCCTAGCGGT

GGTGGTGGTGGTGAT

CATAGGTTGAATAATCAACCGAATCGGGTTGGGAATAATATGTATGCTTCGTCTCTA

GGGTTGCAAAGGC

AATCCAGTGGGAGTAGTTTCGGTGAGAGCTCTTTGTCTGGGGATTATTACATGCCTA

CGCTTTCTGCGGC

GGCTAACGAGATCGAATCTGTTGGATTTCCTCAAGATGATGGGTTTAGGCTTGGATT

TGGTGGTGGTGGA

GGAGATTTGAGGATACAGATGGCGGCGGACTCCGCTGGAGGGTCTTCATCTGGGA

AGAGCTGGGCGCAGC

AGACGGAGGAGAGTTATCAGCTGCAGCTTGCATTGGCGTTAAGGCTTTCGTCGGAG

GCTACTTGTGCCGA

CGATCCGAACTTTCTGGATCCTGTACCGGACGAGTCTGCTTTACGGACTTCGCCAA

GTTCAGCCGAAACC

GTTTCACATCGTTTCTGGGTTAATGGCTGCTTATCGTACTATGATAAAGTTCCTGATG

GGTTTTATATGA

TGAATGGTCTGGATCCCTATATTTGGACCTTATGCATCGACCTGCATGAAAGTGGTC

GCATCCCTTCAAT

TGAATCATTAAGAGCTGTTGATTCTGGTGTTGATTCTTCGCTTGAAGCGATCATAGTT

GATAGGCGTAGT

GATCCAGCCTTCAAGGAACTTCACAATAGAGTCCACGACATATCTTGTAGCTGCATT

ACCACAAAAGAGG

TTGTTGATCAGCTGGCAAAGCTTATCTGCAATCGTATGGGGGGTCCAGTTATCATG

GGGGAAGATGAGTT

GGTTCCCATGTGGAAGGAGTGCATTGATGGTCTAAAAGAAATCTTTAAAGTGGTGGT

TCCCATAGGTAGC

CTCTCTGTTGGACTCTGCAGACATCGAGCTTTACTCTTCAAAGTACTGGCTGACATA

ATTGATTTACCCT

GTCGAATTGCCAAAGGATGTAAATATTGTAATAGAGACGATGCCGCTTCGTGCCTTG

TCAGGTTTGGGCT

TGATAGGGAGTACCTGGTTGATTTAGTAGGAAAGCCAGGTCACTTATGGGAGCCTG

ATTCCTTGCTAAAT

GGTCCTTCATCTATCTCAATTTCTTCTCCTCTGCGGTTTCCACGACCAAAGCCAGTT

GAACCCGCAGTCG

ATTTTAGGTTACTAGCCAAACAATATTTCTCCGATAGCCAGTCTCTTAATCTTGTTTT

CGATCCTGCATC

AGATGATATGGGATTCTCAATGTTTCATAGGCAATATGATAATCCGGGTGGAGAGAA

TGACGCATTGGCA

GAAAATGGTGGTGGGTCTTTGCCACCCAGTGCTAATATGCCTCCACAGAACATGAT

GCGTGCGTCAAATC

AAATTGAAGCAGCACCTATGAATGCCCCACCAATCAGTCAGCCAGTTCCAAACAGG

GCAAATAGGGAACT

TGGACTTGATGGTGATGATATGGACATCCCGTGGTGTGATCTTAATATAAAAGAAAA

GATTGGAGCAGGT

TCCTTTGGCACTGTCCACCGTGCTGAGTGGCATGGCTCGGATGTTGCTGTGAAAAT

TCTCATGGAGCAAG

ACTTCCATGCTGAGCGTGTTAATGAGTTCTTAAGAGAGGTTGCGATAATGAAACGCC

TTCGCCACCCTAA

CATTGTTCTCTTCATGGGTGCGGTCACTCAACCTCCAAATTTGTCAATAGTGACAGA

ATATTTGTCAAGA

GGTAGTTTATACAGACTTTTGCATAAAAGTGGAGCAAGGGAGCAATTAGATGAGAGA

CGTCGCCTGAGTA

TGGCTTATGATGTGGCTAAGGGAATGAATTATCTTCACAATCGCAATCCTCCAATTG

TGCATAGAGATCT

AAAATCTCCAAACTTATTGGTTGACAAAAAATATACAGTCAAGGTTTGTGATTTTGGT

CTCTCGCGATTG

AAGGCCAGCACGTTTCTTTCCTCGAAGTCAGCAGCTGGAACCCCCGAGTGGATGG

CACCAGAAGTCCTGC

GAGATGAGCCGTCTATGAAAAGTCAGATGTGTACAGCTTCGGGGTCATCTTGTGG

GAGCTTGCTACATT

GCAACAACCATGGGGTAACTTAAATCCGGCTCAGGTTGTAGCTGCGGTTGGTTTCA

AGTGTAAACGGCTG

GAGATCCCGCGTAATCTGAATCCTCAGGTTGCAGCCATAATCGAGGGTTGTTGGAC

CAATGAGCCATGGA

AGCGTCCATCATTTGCAACTATAATGGACTTGCTAAGACCATTGATCAAATCAGCGG

TTCCTCCGCCCAA

CCGCTCGGATTTGTAA

＞KO175352 gi|7340658|emb|CAB82938.1| serine/threonine protein kitase CTR1[Arabidopis thaliana]

MEMPGRRSNYTLLSQFSDDQVSVSVTGAPPPHYDSLSSENRSNHNSGNTGKAKAERG

GFDWDPSGGGGGD

HRLNNQPNRVGNNMYASSLGLQRQSSGSSFGESSLSGDYYMPTLSAAANEIESVGFP

QDDGFRLGFGGGG

GDLRIQMAADSAGGSSSGKSWAQQTEESYQLQLALALRLSSEATCADDPNFLDPVPDE

SALRTSPSSAET

VSHRFWVNGCLSYYDKVPDGFYMMNGLDPYIWTLCIDLHESGRIPSIESLRAVDSGVDS

SLEAIIVDRRS

DPAFKELHNRVHDISCSCITTKEVVDQLAKLICNRMGGPVIMGEDELVPMWKECIDGLK

EIFKVVVPIGS

LSVGLCRHRALLFKVLADIIDLPCRIAKGCKYCNRDDAASCLVRFGLDRFYLVDLVGKPG

HLWEPDSLLN

GPSSISISSPLRFPRPKPVEPAVDFRLLAKQYFSDSQSLNLVFDPASDDMGFSMFHRQY

DNPGGENDALA

ENGGGSLPPSANMPPQNMMRASNQIEAAPMNAPPISQPVPNRANRELGLDGDDMDIP

WCDLNIKEKIGAG

SFGTVHRAEWHGSDVAVKILMEQDFHAERVNEFLREVAIMKRLRHPNIVLFMGAVTQP

PNLSIVTEYLSR

GSLYRLLHKSGAREQLDERRRLSMAYDVAKGMNYLHNRNPPIVHRDLKSPNLLVDKKY

TVKVCDFGLSRL

KASTFLSSKSAAGTPEWMAPEVLRDEPSNEKSDVYSFGVILWELATLQQPWGNLNPAQ

VVAAVGFKCKRL

EIPRNLNPQVAAIIEGCWTNEPWKRPSFATIMDLLRPLIKSAVPPPNRSDL

At2g42690，SEQ ID No.107

＞KO-T3-02-29765-1 At2g42690

atggctacaacaaccacatcatgggaagaactcttaggctcaaagaattgggacactatcttagacccattagaccaatca

cttagggaactcatcttacgttgtggcgacttttgtcaagccacctacgatgccttcgtcaacgaccaaaactccaagtactgt

ggagccagccgctacggcaaatcttctttcttcgacaaggtcatgctcgaaaacgcttccgactacgaggttgtaaacttcct

ctacgccacagctcgtgtttctctccccgaaggtttgcttctccaatcacaatcaagagattcttgggaccgtgagtctaactgg

tttggctacattgctgtcacgtctgatgaacggtctaaggctttaggacgccgtgagatctatatagctttgagaggaacgagc

aggaactatgagtgggtcaatgttttgggtgctaggccaacttcagctgaccccttgctgcacggacccgagcaggatggtt

ctggtggtgtagttgaaggtacgacttttgatagtgacagtgaagatgaagaagggtgtaaggtgatgctcgggtggctcac

aatctatacttctaatcaccccgaatcgaaattcactaagctgagtctacggtcacagttgttagccaagatcaaggagcttct

gttgaagtataaggacgagaaaccgagcattgtgttgactggacatagcttgggagctacagaggctgttctggccgcctat

gatatagctgagaacggttccagtgatgatgttccggtcactgctatagtctttggttgtccacaggtaggaaacaaggagttc

agagacgaagtaatgagtcacaagaacttaaagatcctccatgtaaggaacacgattgatctcttaactcgatacccaggg

ggacttttagggtatgtggacataggaataaactttgtgatcgatacaaagaagtcaccgttcctaagcgattcaaggaatcc

aggggattggcataatcttcaggcgatgttacatgttgtagctggatggaatgggaagaaaggagagtttaaactgatggtta

agagaagtattgcattagtgaacaagtcatgcgagttcttgaaagctgagtgtttggtgccaggatcttggtgggtagagaag

aacaaaggactgatcaagaacgaagatggtgaatgggttcttgctcccgttgaagaagaacctgtacctgaattctaa

The lipase [Arabidopis thaliana] of＞KO29765 gi|4512683|gb|AAD21737.1| supposition

MATTTTSWEELLGSKNWDTILDPLDQSLRELILRCGDFCQATYDAFVNDQNSKYCGAS

RYGKSSFFDKVM

LENASDYEVVNFLYATARVSLPEGLLLQSQSRDSWDRESNWFGYIAVTSDERSKALGR

REIYIALRGTSR

NYEWVNVLGARPTSADPLLHGPEQDGSGGVVEGTTFDSDSEDEEGCKVMLGWLTIYT

SNHPESKFTKLSL

RSQLLAKIKELLLKYKDEKPSIVLTGHSLGATEAVLAAYDIAENGSSDDVPVTAIVFGCPQ

VGNKEFRDE

VMSHKNLKILHVRNTIDLLTRYPGGLLGYVDIGINFVIDTKKSPFLSDSRNPGDWHNLQA

MLHWAGWNG

KKGEFKLMVKRSIALVNKSCEFLKAECLVPGSWWVEKNKGLIKNEDGEWVLAPVEEEP

VPEF

At4g31810 SEQ ID No.109

＞KO20 (gi|4584519) Arabidopis thaliana DNA karyomit(e) 4, BAC clones F11C18 (ESSA plan)

ATGCAAACAGTGAAAGCTTTGAGGAGAGTGAGTGAACCCTTACAATGGGTTCGGTC

TGTTTCTTATGGAA

GACGCTTTTCTGCTCTCCCAAACTATTCCGCATCAGATGCAGATTTCGAAGACCAGG

TTCTGGTGGAAGG

AAAAGCTAAATCAAGAGCTGCCATTCTCAATAACCCATCTTCTCTCAATGCTCTTTCT

GCGCCTATGGTA

TTGTGTTCACCAGATTATGCTTCAAAAACTTTTGCCTTGGTAGGTTGGTCGGTTAAA

GAGGCTATACGAA

TCATGGGAAGAGAACCCAGCTATTTCCTTTGTTTTGATGAAGGAAATACTGAAGAAT

CTAAACTCTTTTT

CGAGAACTTGTACAAGTTTGTATACCTCCAAGGAACGTATTTAAACCAAATATAGC

AATAATGGATGGT

GTGACCATGGGTTGTGGTGGTGGAATTTCACTTCCAGGGATGTTTCGTGTGGCTAC

AGATAAAACTGTGT

TGGCCCATCCAGAGGTCCAAATTGGTTTTCATCCTGATGCAGGAGCTTCCTATTATC

TTTCACGGCTTCC

TGGTTATTTAGGGGAATACTTGGCTCTAACGGGGCAGAAACTTAATGGTGTCGAAAT

GATAGCATGTGGC

CTTGCCACCCACTATTGCTTAAACGCGAGACTTCCGTTGATTGAAGAGAGGATTGGT

AAACTGTTGACCG

ATGATCCTGCTGTCATTGAGGATTCTCTTGCTCAATATGGTGATCTTGTTTACCCTGA

CAGTAGCAGCGT

ACTGCACAAGATAGAGTTGATTGATAAATATTTTGGGCTTGATACCGTTGAAGAAAT

CATTGAAGCTATG

GAAAATGAAGCTGCTAATTCGTGCAATGAATGGTGCAAGAAAACTCTCAAACAGATC

AAAGAAGCTTCAC

CTTTGAGCTTAAAGATTACTTTGCAATCTATACGAGAAGGTAGATTCCAAACCCTTGA

TCAATGTCTCAC

ACATGAATACCGTATATCCATTTGTGGAGTCTCAAAAGTAGTCTCTGGCGACTTTTG

CGAGGGTATTCGA

GCCCGTTTGGTAGATAAAGACTTTGCTCCAAAGGTGCATACAAACATATCAGCCTCA

AAATTAGACTGGG

ATCCTCCACGCCTAGAAGATGTGAGCAAAGACATGGTGGATTGCTACTTCACGCCA

GCCTCAGAGCTCGA

TGATTCAGATTCTGAGTTGAAGCTGCCAACAGCTCAACGAGAGCCTTATTTTTGA

＞KO20 gi|4584520|emb|CAB40751.1| alkene acyl-CoA hydratase sample albumen [Arabidopis thaliana]

MQTVKALRRVSEPLQWVRSVSYGRRFSALPNYSASDADFEDQVLVEGKAKSRAAILNN

PSSLNALSAPMV

LCSPDYASKTFALVGWSVKEALRIMGREPSYFLCFDEGNTEESKLFFENLYKFVYLQGT

YLKPNIAIMDG

VTMGCGGGISLPGMFRVATDKTVLAHPEVQIGFHPDAGASYYLSRLPGYLGEYLALTG

QKLNGVEMIACG

LATHYCLNARLPLIEERIGKLLTDDPAVIEDSLAQYGDLVYPDSSSVLHKIELIDKYFGLDT

VEEIIEAM

ENEAANSCNEWCKKTLKQIKEASPLSLKITLQSIREGRFQTLDQCLTHEYRISICGVSKVV

SGDFCEGIR

ARLVDKDFAPKVHTNISASKLDWDPPRLEDVSKDMVDCYFTPASELDDSDSELKLPTAQ

REPYF

Alkene acyl-CoA the hydratase of＞BN45665575 supposition

atgcaaacagtgagagctttgaggagagtcactaaaccctcacaatgggttcggtctgtttcccaaggaaaaagaagcttct

ccgccctaccaaacttctccgcttcagatgccgatgaagaccaggtttcggttgaagggaaagctaaatcaagagccg

ccattctcgatagaccctcttcactcaatgctcttttctgctcccatggttggtcggttgaagaggctatacgagtcatgggaaga

gaaccctgctatttcgtttgttttgatgaagggtagcggaaaaacgttctgttctggtgcagatgtcttgcctctttatcactcggatc

aatgaagggaatactgaagaatgtaaacactttttcgggagcttgtacaattttgtatacctccaaggaacatatttgaaacca

aatatagctataatggatggtgtaacaatgggttgtggtggtggcatttcaattccagggatgtttcgtgtggcaacagataaa

actgtgttggcacatccagaggttcaaattggttttcatcctgatgctggagcttcttattacctttcacggcttcctggctatttagg

ggaatacttggctctaacagggcagaaacttgatggagtcaaaatgatagcatgtggccttgccacccacttttgcctacact

cgagacttgggatggtcgaagagaggattggtaagctgttgacagatgatccaactgtcattgaggcttctcttgctcaatac

agtgatctagtttatcctgacaataccagtgtacttcacaagatcgagatgattgatagatactttgggcttgacacggttgaag

aaatcattgaggctatggaaaacgaggttgctgattctggcaatgaatggtgcaagaaaactctcaaacaagtcaaagaa

gcttctcctttgagcttaaagattactttacaatctatacgagaaggtagatttcaaactcttgatcagtgtctcacgcgtgagtac

cgtatctctctctgtggagtctcaaagactgtctctggtgacttctgcgagggtattcgagcccgtttggtggataaagactttgct

ccaaagtgggatcctccgcgcctagaagatgtaagcaaagacatggtggactgctacttctcgccagccacagatgccga

tgattcagaatctgagctgaagcttccaacagctcaacgagagccttacttctga

Alkene acyl-CoA the hydratase of＞BN45665575 supposition

mqtvralrrvtkpsqwvrsvsqgkrsfsalpnfsasdadvqdqvsvegkaksraaildrpsslnalsapmvgrlkrlyeswe

enpaisfvlmkgsgktfcsgadvlplyhsinegnteeckhffgslynfvylqgtylkpniaimdgvtmgcgggisipgmfrvat

dktvlahpevqigfhpdagasyylsrlpgylgeylaltgqkldgvkmiacglathfclhsrlgmveerlgklltddptvleasaq

ysdlvypdntsvlhkiemidryfgldtveeiieamenevadsgnewckktlkqvkeasplslkitlqsiregrfqtldqcltrey ri

slcgvsktvsgdfcegirarlvdkdfapkwdpprledvskdmvdcyfspatdaddseselklptaqrepyf*

Alkene acyl-CoA the hydratase of＞GM59573001 supposition

atgcagagattcaaagctctgctacctcaacaaactaggtcctcacttcgcactctctgttctcaccgtcgagctttctccgctc

aaccgaattacgcaaagcaccacgacgacgattctcaggaacagattttagtcgaaggaagagcgaaatcacgagcag

ctattctcaacaggccgtcttcgctgaactcgctcaatgcttcaatggttgctcggttgaagaggctgtatgattcctgggaaga

aaactctgatattggctttgttttgatgaagggtagtggcagagctttctgttctggtgcagatgttgttaggctgtatcactcactc

aatgaaggaaatactgacgaagctgaacagtttttcaaaacattatattcatttgtatatcttcaagggacatatcttaaaccac

atgttgccattttggatggaataacaatgggatgtggatctggaatttctctaccaggaatgttccgtgtggtaactgataaaact

gttttttctcacccagaagctcaaataggtttccacccagatgcaggagcttcttatgttttgtctcgtctacctggctacttagggg

aatacttggcccttacaggagataagcttaatggtgttgaaatgattgcctgccgccttgctactcattattcactaaatgcaag

gctctctttgcttgaagaacgtcttggtaaactaatcacagacgaaccttctgttgtggagtcatccctcgcacagtatggtgatc

ttgtttatccagataggagcagtgtccttcacaggattgatactattgatagatgtttcagtcacgaaactgtggaggaaattatt

gaagctttggagaaagaggctgctgagtctaatgacgaatggtactcgactactctaaggagaataagagaagcctcccc

gttgagtttgaaagttactttacaatctatacgtgaaggtagatttgaaacacttgataaatgtcttgtacgtgagtatcgcatgtc

cctacgtggtatttcaaagcatgtctcctctgatttctttgagggtgttcgggcacgaatggttgatagagattttgcaccaaagtg

ggacccacctagattaaaagatatatcagaggacatggttgaatactatttctctcctttaagtgaagttcaatctgaattagtg

ctgccaacagctttgcgagaaccttacatgtga

Alkene acyl-CoA the hydratase of＞GM59573001 supposition

mqrfkallpqqtrsslrtlcshrrafsaqpnyakhhdddsqeqilvegraksraailnrpsslnslnasmvarlkrlydsween

sdigfvlmkgsgrafcsgadvvrlyhslnegntdeaeqffktlysfvylqgtylkphvaildgitmgcgsglslpgmfrvvtdktv

fshpeaqlgfhpdagasyvlsrlpgylgeylaltgdklngvemiacrlathyslnarlslleerlgklitdepsvvesslaqygdlv

ypdrssvlhrid-drcfshetveeiiealekeaaesndewysttlrrireasplslkvtlqsiregrfetldkclvreyrmslrgiskh

vssdffegvrarmvdrdfapkwdpprlkdlsedmveyyfsplsevqselvlptalrepym*

At4g31820，SEQ ID No.111

＞KO20 (gi|4584519) Arabidopis thaliana DNA karyomit(e) 4, BAC clones F11C18 (ESSA plan)

ATGCCAGGAGGATACAAAGCGTTTGAGATCTGTGCCAAGTTTTGCTATGGGATGAC

TGTTACGCTCAATG

CTTACAACATAACCGCGGTGCGATGTGCAGCTGAGTATCTTGAAATGACTGAAGAT

GCTGACCGCGGTAA

CCTCATATACAAGATCGAAGTTTTCCTCAACTCAGGCATATTCAGAAGCTGGAAAGA

CTCAATCATTGTG

CTTCAGACAACAAGATCTCTTCTTCCTTGGTCTGAAGATCTGAAGCTTGTTGGTAGA

TGCATAGATTCTG

TTTCAGCTAAGATCTTGGTGAACCCTGAGACTATCACTTGGTCTTATACATTCAACA

GGAAGTTATCTGG

ACCTGATAAGATAGTCGAATATCATCGGGAGAAGAGAGAAGAGAATGTGATTCCGA

AAGATTGGTGGGTC

GAAGATGTATGTGAGCTAGAGATTGATATGTTCAAGAGGGTGATAAGTGTTGTGAAA

TCTAGTGGAAGGA

TGAATAATGGCGTAATTGCTGAAGCTCTTAGATACTATGTTGCAAGGTGGTTACCAG

AATCTATGGAGTC

TTTGACATCAGAAGCTTCTTCAAACAAAGATCTCGTTGAGACGGTTGTTTTCTTGTTG

CCGAAGGTAAAC

AGAGCAATGAGCTACTCTTCTTGCAGCTTCTTGCTAAAACTCCTTAAAGTTTCGATCT

TGGTTGGAGCTG

ATGAGACGGTGAGAGAAGATTTGGTTGAGAACGTGAGTTTGAAGCTTCATGAAGCG

TCCGTTAAAGATTT

GCTGATCCATGAAGTCGAATTAGTCCATCGGATTGTTGATCAGTTCATGGCGGATGA

GAAACGTGTATCT

GAAGATGACCGGTACAAGGAGTTTGTTTTAGGAAATGGAATTTTGTTGAGTGTAGGA

AGATTGATTGATG

CTTATCTCGCTCTTAACTCTGAACTTACACTCTCTAGCTTTGTTGAGTTATCTGAGTT

AGTCCCGGAATC

AGCTAGGCCGATACACGACGGTCTCTACAAAGCCATTGACACTTTCATGAAGGAAC

ATCCCGAACTAACA

AAATCCGAAAAGAAGAGGCTTTGTGGGTTAATGGACGTGAGGAAACTGACAAATGA

AGCATCAACGCACG

CTGCACAGAACGAGAGACTTCCACTACGAGTGGTGGTGCAAGTTCTCTACTTTGAG

CAGCTCCGAGCAAA

TCACAGCCCCGTGGCGTCTGTTGCGGCTTCGTCACACTCGCCGGTTGAGAAGACG

GAGGAGAACAAAGGA

GAAGAAGCGACGAAGAAGGTGGAGCTGAGCAAGAAAAGCAGAGGAAGCAAGAGCA

CGAGGAGTGGTGGTG

GTGCACAGCTGATGCCGTCGAGGTCAAGGAGGATCTTTGAGAAGATATGGCCTGG

GAAAGGAGAGATTAG

CAACAAGAGCTCTGAGGTTTCTTCTGGAAGCTCACAAAGTCCGCCAGCCAAGTCTT

CTAGCTCGTCTTCC

CGACGCCGCAGACATTCGATATCGTGA

The albumen [Arabidopis thaliana] of＞KO20 gi|4584521|emb|CAB40752.1| supposition

MPGGYKAFEICAKFCYGMTVTLNAYNITAVRCAAEYLEMTEDADRGNLIYKIEVFLNSGI

FRSWKDSIIV

LQTTRSLLPWSEDLKLVGRCIDSVSAKILVNPETITWSYTFNRKLSGPDKIVEYHREKRE

ENVIPKDWWV

EDVCELEIDMFKRVISVVKSSGRMNNGVIAEALRYYVARWLPESMESLTSEASSNKDLV

ETVVFLLPKVN

RAMSYSSCSFLLKLLKVSILVGADETVREDLVENVSLKLHEASVKDLLIHEVELVHRIVDQ

FMADEKRVS

EDDRYKEFVLGNGILLSVGRLIDAYLALNSELTLSSFVELSELVPESARPIHDGLYKAIDTF

MKEHPELT

KSEKKRLCGLMDVRKLTNEASTHAAQNERLPLRVVVQVLYFEQLRANHSPVASVAASS

HSPVEKTEENKG

EEATKKVELSKKSRGSKSTRSGGGAQLMPSRSRRIFEKIWPGKGEISNKSSEVSSGSS

QSPPAKSSSSSS

RRRRHSIS

KO02173 At5g39470

＞KO02173 gi|18421869:1-513 Arabidopis thaliana F-box protein family (At5g39470) mRNA,

Complete encoding sequence

ATGGTTCTTGCCAGGCTGATCTTCCAAGCAACGATCTATCCCATTTGGCTAGACAAA

ACGGAGGCGTCCG

ACATCAGCAAGCTAGCCACCCAGTTTGGTACATTGAGACTCATCGATGAAGCTATTA

GTGGGAAACTTGC

CTCATACACATCGTACGAACATCTCCAACTAGAAGCTTTAATTGCTTGGTTCCACCA

TCTTCAACCTAAA

TTTGAAAACAACCTAAACGAGAATACCTCAAAGTCTGCGTTATCTTCTGAATTCTGTA

AGGTTGGTGCTT

GCTTGCTTCTTACGCTTCCCGAAGATGTGTTTTCTGTTATCTCTCACTTTCTTTCTCC

AAGCGACATTTG

CGATATAATCTTTTGCTGCAAAAGTCTTTGTGCCCTTGTCGATTCCGAGAAGACATG

GCTTGTTCAATAT

GAAGTCGTTAAGGTGGTGAAGCCTCTTGTTGGGATTTGGGTTCAAAAGAACCCTGT

AATTGGGATTTCTT

ATCCGTTGTTGGATGCCGGATAA

＞KO02173 gi|15241754|ref|NP_198763.1|F-box protein family [Arabidopis thaliana]

MVLARLIFQATIYPIWLDKTEASDISKLATQFGTLRLIDEAISGKLASYTSYEHLQLEALIAW

FHHLQPK

FENNLNENTSKSALSSEFCKVGACLLLTLPEDVFSVISHFLSPSDICDIIFCCKSLCALVDS

EKTWLVQY

EVVKVVKPLVGIWVQKNPVIGISYPLLDAG

KO03466

＞GM59650787 agnoprotein

atgtctgtggaaaggtcgtttgaggcatgggaagaggtgcagcgtcacgggcaggacctagctgaccgtcttgcccagggt

tttagcggtttgattcacacgcatatgagccctccgcaattcgcgtggccgaaccctccgacatcgaagctcttcgatctggag

ttcccttcgcagaactttgggaagagggatttcgctttggcgacccaggagtacgggattaatggcgtgtcagcgatttttgac

atcgggaatcggatcggtcaggccggggcggatttcggtgccagcttgaacgggctggttcagcagtttttccggtcgttgcc

ggtgccgatgccattcaagcacgaggagagttcagtgagggtggagggtggggataaggggtggcagagaggagggg

ttgtggttgctgtgcaggaggatttgggattgcttagtgagaggttgaagaatcgtgggtttgctgagagtgttagtggcagtggt

ggtggaagcgcggaggaagagggtggtggagggtttaaccttgggtctattggtcttctgggcaggcgacagggaatcata

aattttacatcaacttatgatagtagaactcaagaagtggaaggttctttagttgcaaggggagatttgtggagagtagaggc

atcacatggtggttctgcgtctagaaatgaaaattcatctcttttcctggttcagcttggacctcttctcttttatccgtgattcaactct

cctcttgcctgttcatttgtcaaagcagcacttgctgtggtatggctatgatagaaagaatggaatgcattctctttgtccagcagt

gtggtcaaaacacagaaggtggctgttaatgtccatgctttgcctgaatcccctagcttgttcatttgtggatcttcaattccctaa

tgggcaactaacctacgtatctggagagggtctaagtaccagtgctttccttcctgtttatggaggtcttcttcaagctcagggtc

aatatcctggggaaatgagattcagcttttcgtgcaagaataagtggggaacaagaatcacaccaatggtacaatggcctg

acaaatcattttctttgggtcttgctcaagccttggcctggaagcgatctggtctaatggtgaggccatctgttcaattcagtgtgt

gtcctactgttggtggaagcaatccagggttgcgggcagaactcattcattcagttaaagagaaacttaatctaatttgtggat

gtgctttcatgacatatccttctgcctttgcttcagtatctattggaagatcaaagtggaatggaaatgtggggaactcgggtcta

gttctaagagttgatgttcctctctccaccgttgggcgcccttccttctccgttcagataaatagtggcattgagttttga

＞GM59650787 agnoprotein

msversfeaweevqrhgqdladrlaqgfsglihthmsppqfawpnpptsklfdlefpsqnfgkrdalatqeygingvsaifd

lgnrigqagadfgaslnglvqqffrslpvmpfkheessvrveggdkgwqrggvvvavqedlgllserlknrgfaesvsgsg

ggsaeeeggggfnlgslglgrrqgiinftstydsrtqevegslvargdlwrveashggsasmensslflvqlgpllfirdstlllpv

hlskqhllwygydrkngmhslcpavwskhrrwllmsmlclnplacsfvdlqfpngqltyvsgeglstsaflpvyggllqaqgq

ypgemrfsfscknkwgtritpmvqwpdksfslglaqalawkrsglmvrpsvqfsvcptvhgsnpglraelihsvkeklnlicg

cafmtypsafasvslgrskwngnvgnsglvlrvdvplstvgrpdgsvqinsglef

KO10625 At3g49110

＞KO10625 gi|30693139:50-1114 Arabidopis thaliana peroxidase (At3g49110) mRNA,

Complete encoding sequence

ATGCAATTCTCTTCATCTTCTATTACTTCTTTCACTTGGACAGTTTTAATCACAGTGG

GATGTCTTATGC

TTTGTGCGTCTTTCTCCGATGCTCAACTTACCCCTACTTTTTACGACACTTCATGTCC

TACCGTCACCAA

CATTGTAAGAGATACCATTGTCAACGAGCTAAGATCGGACCCTCGTATCGCCGGGA

GCATCCTTCGTCTT

CACTTCCATGACTGCTTTGTTAATGGTTGTGATGCTTCGATCTTGTTAGACAACACG

ACATCATTTCGAA

CAGAGAAAGATGCACTTGGAAATGCAAATTCAGCCCGAGGATTTCCAGTGATTGATA

GAATGAAAGCTGC

GGTGGAGAGGGCATGCCCAAGAACCGTTTCATGCGCAGATATGCTCACCATTGCTG

CTCAACAATCTGTC

ACTTTGGCAGGAGGTCCTTCTTGGAAGGTTCCTTAGGGAGAAGAGACAGCTTACA

AGCATTTCTAGATC

TTGCTAACGCAAATCTTCCAGCTCCATTCTTCACACTTCCACAGCTTAAAGCCAACTT

CAAAAATGTTGG

CCTCGATCGTCCTTCTGATCTTGTTGCGCTCTCCGGGGCTCACACATTTGGTAAAAA

TCAATGTCGATTC

ATTATGGACAGATTATACAACTTTAGCAACACTGGATTACCTGACCCTACACTCAAC

ACTACTTACCTCC

AAACTCTTCGTGGTCAATGTCCTCGCAATGGTAATCAAAGCGTCTTAGTGGATTTCG

ATCTGCGTACGCC

TTTGGTTTTCGACAACAAATACTATGTGAATCTTAAAGAGCAAAAAGGTCTTATCCAG

AGCGACCAAGAG

TTGTTCTCTAGCCCCAATGCCACTGACACAATCCCCTTGGTGAGAGCATATGCTGAT

GGCACACAAACAT

TCTTCAATGCATTCGTGGAGGCAATGAATAGGATGGGAAATATTACACCAACTACAG

GAACTCAAGGACA

AATCAGGTTGAATTGTAGAGTGGTGAACTCCAACTCTCTACTCCATGATGTGGTGGA

TATCGTTGACTTT

GTAAGTTCTAATGTGA

＞KO0625 gi|15229084|ref|NP_90480.1| peroxidase [Arabidopis thaliana]

MQFSSSSITSFTWTVLITVGCLMLCASFSDAQLTPTFYDTSCPTVTNIVRDTIVNELRSDP

RIAGSILRL

HFHDCFVNGCDASILLDNTTSFRTEKDALGNANSARGFPVIDRMKAAVERACPRTVSCA

DMLTIAAQQSV

TLAGGPSWKVPLGRRDSLQAFLDLANANLPAPFFTLPQLKANFKNVGLDRPSDLVALS

GAHTFGKNQCRF

IMDRLYNFSNTGLPDPTLNTTTYLQTLRGQCPRNGNQSVLVDFDLRTPLVFDNKYYVNLK

EQKGLIQSDQE

LFSSPNATDTIPLVRAYADGTQTFFNAFVEAMNRMGNITPTTGTQGQIRLNCRVVNSNS

LLHDVVDIVDF

VSSM

KO10625 At3g49120

＞KO10625 gi|30693142:169-1230 Arabidopis thaliana peroxidase, (At3g49120) that supposes

MRNA, complete encoding sequence

ATGCATTTCTCTTCGTCTTCAACATCGTCCACTTGGACAATCTTAATCACATTGGGAT

GTCTTATGCTTC

ATGCATCTTTGTCCGCTGCTCAACTCACCCCTACCTTCTACGATAGGTCATGTCCTA

ATGTCACTAACAT

CGTACGAGAAACCATTGTAAATGAGTTAAGGTCGGACCCTCGTATCGCTGCGAGCA

TCCTTCGTCTTCAC

TTCCACGACTGCTTTTTAATGGTTGTGACGCATCCATCTTGTTAGACAACACGACA

TCATTTCGAACAG

AGAAAGATGCGTTTGGAAACGCAAATTCGGCTCGGGGATTTCCAGTGATTGATAGA

ATGAAAGCTGCGGT

GGAGAGGGCATGCCCAAGAACCGTTTCATGCGCAGATATGCTCACCATTGCAGCTC

AACAATCTGTCACT

TTGGCAGGAGGTCCTTCTTGGAGGGTTCCTTTGGGAAGGAGAGACAGTTTACAAGC

ATTCCTGGAACTCG

CTAATGCAAATCTTCCAGCTCCATTCTTTACACTTCCACAACTTAAAGCCAGCTTCAG

AAATGTTGGTCT

CGATCGTCCTTCTGATCTCGTTGCTCTCTCCGGTGGTCACACATTTGGTAAAAATCA

ATGTCAGTTTATT

CTTGACAGATTATACAATTTCAGCAACACAGGTTTACCCGACCCTACACTCAACACT

ACTTACCTCCAAA

CTCTTCGTGGACTATGCCCCCTTAATGGCAATCGAAGTGCCTTGGTAGATTTTGATC

TACGTACGCCTAC

GGTTTTCGACAACAAATACTACGTGAATCTCAAAGAGCGAAAAGGTCTTATCCAGAG

CGACCAAGAGTTG

TTCTCTAGCCCCAATGCCACTGACACAATCCCCTTGGTGAGAGCATATGCTGATGG

CACACAAACATTCT

TCAATGCATTTGTGGAGGCAATGAATAGGATGGGAAACATTACACCAACTACAGGAA

CTCAAGGACAAAT

CAGATTGAACTGTAGAGTTGTGAACTCCAACTCTCTGCTCCATGATGTGGTGGATAT

CGTTGACTTTGTT

AGCTCTATGTGA

＞KO10625 gi|15229095|ref|NP_190481.1| peroxidase, [Arabidopis thaliana] supposed

MHFSSSSTSSTWTILTTLGCLMLHASLSAAQLTPTFYDRSCPNVTNIVRETIVNELRSDPR

IAASILRLH

FHDCFVNGCDASILLDNTTSFRTEKDAFGNANSARGFPVIDRMKAAVERACPRTVSCA

DMLTIAAQQSVT

LAGGPSWRVPLGRRDSLQAFLELANANLPAPFFTLPQLKASFRNVGLDRPSDLVALSG

GHTFGKNQCQFI

LDRLYNFSNTGLPDPTLNTTYLQTLRGLCPLNGNRSALVDFDLRTPTVFDNKYYVNLKE

RKGLIQSDQEL

FSSPNATDTIPLVRAYADGTQTFFNAFVEAMNRMGNITPTTGTQGQIRLNCRVVNSNSL

LHDVVDIVDFV

SSM

KO11479 At4g16930

The disease-resistant albumen of＞KO11479 gi|18414779:1-465 Arabidopis thaliana (TIR-NBS class) is supposed

(At4g16930) mRNA, complete encoding sequence

ATGGTGACTCCGATTTTCTACGAGGTTGATCATTCTGATGTTAGGAAACAGACCGGA

GAATTTGGAAAGG

TCTTTGAAGAGACATGCAAGAACAAAACAGATGATGAGAACAAAGGTGTAGGAAA

GCTCTAGCAGATGT

GGCAAATATGGCTGGAGAGGATTCTCGAAACTGGTGTAATGAAGCAAACATGATTG

AAACAATTTCCAAC

GATGTTCCGAATAAGCTCATAACACCATCGAGTGATTTAGGTGATTTCGTTGGTGTT

GAAGCTCATTTTAG

AGAGATTGAGTTCATTGTTGTGCTTGGAATCTGAAGAAGCTAGAATGGTAGGGATTG

GTAAGAGTACCCT

AGGAAGAGCTCTTTTCAGTCAACTCTCTAGCCAATTCCCCCTTCGCGCTTTCGTAAC

TTATAAACCAACC

GAGAAGAACAGGTTTTATCAGAAATTTTATGTCAAAGGACATAA

The disease-resistant albumen of＞KO11479 gi|15235929|ref|NP_93426.1| (TIR-NBS class), [Arabidopis thaliana] supposed

MVTPIFYEVDHSDVRKQTGEFGKVFEETCKNKTDDEKQRCRKALADVANMAGEDSRN

WCNEANMIETISN

DVPNKLITPSSDLGDFVGVEAHLERLSSLLCLESEEARMVGIGKSTLGRALFSQLSSQFP

LRAFVTYKPT

EKNRFYQKFYVKRT

KO11479 At4g16940

The disease-resistant albumen of＞KO11479 gi|18414780:1-3312 Arabidopis thaliana (TIR-NBS-LRR class) is supposed

(At4g16940) mRNA, complete encoding sequence

ATGGCTAGCCGGAGATACGACGTTTTCCCAAGCTTCAGTGGGGTAGATGTTCGCAA

AACGTTCCTCAGCC

ATCTAATCGAGGCGCTCGACCGCAGATCAATCAATACATTCATGGATCACGGCATC

GTGAGAAGCTGCAT

AATCGCCGATGAGCTTATAAACGGCCATTAGAGAAGCGAGGATCTCAATAGTTATCTT

CTCTGAGAACTAT

GCTTCTTCCACGTGGTGCTTGAATGAATTGGTGGAGATCCACAAGTGTCACAAGGA

CAAAGACTTGGATC

AAATGGTGATTCCGGTTTTCTACGGCGTTGATCCTTCTCATGTTAGAAAACAGATCG

GTGGCTTTGGCGA

TGTCTTTAAAAAGACATGCGAGGACAAACCAGAGGATCAGAAACAAAGATGGGTTA

AAGCTCTCACAGAT

ATATCAAATTTAGCCGGGGAGGATCTTCGGAACGGGCCTAGTGAAGCAGCCATGGT

TGTAAAGATAGCTA

ATGATGTTTCGAATAAACTTTTTCCTCTGCCAAAGGGTTTTGGTGACTTAGTCGGAAT

TGAGGATATAT

AGAGGCAATAAAATTAAAACTGTGCTTGGAATCCAAGGAAGCTAGAATAATGGTCGG

GATTTGGGGACAG

TCAGGGATTGGTAAGAGTACTATAGGAAGAGCTCTTTTCAGTCAACTCTCTAGCCAG

TTCCACCATCGCG

CTTTCATAACTTATAAAAGCACCAGTGGTAGTGACGTCTCTGGCATGAAGTTGAGTT

GGGAAAAAGAACT

TCTCTCGGAAATCTTAGGTCAAAAGGACATAAAGATAGAGCATTTTGGTGTGGTGGA

GCAAAGGTTGAAG

CACAAGAAAGTTCTTATCCTTCTTGATGATGTGGATAATCTAGAGTTTCTTAGGACCT

TGGTGGGAAAAG

CTGAATGGTTTGGATCTGGAAGCAGAATAATTGTGATCACTCAAGATAGGCAACTTC

TCAAGGCTCATGA

GATTGACCTTATATATGAGGTGAAGCTCCCATCTCAAGGTCTTGCTCTTAAGATGAT

ATGCCAATATGCT

TTTGGGAAATACTCTCCACCTGATGATTTTAAGGAACTAGCATTTGAAGTTGCAAAG

CTTGCCGGTAATC

TTCCTTTGGGTCTCAGTGTCCTTGGTTCGTCTTTAAAACGAAGGAGCAAAGAAGAGT

GGATGGAGATGCT

GGCTGAGCTCCAAAATGGTTTGAACAGAGATATTATGAAAACATTAAGAGTCAGCTA

CGTTAGATTAGAT

CCAAAAGATCAAGATATATTCCATTACATTGCATGGTTATTCAATGGTTGGAAAGTCA

AATCCATCAAAG

ACTTCCTCGGAGATGGTGTTAATGTTAACATTAGGCTCAAAACGTTGGATGATAAGT

CCCTCATACGTTT

AACACCGAATGATACTATAGAGATGCACAATTTGCTTCAGAAGTTGGCTACAGAAAT

TGATCGTGAAGAG

TCTAATGGTAATCCTGGAAAACGTCGATTTCTGGAGAATGCTGAGGAAATTCTAGAC

GTATTTACCGATA

ATACCGGCACTGAAAAATTGCTCGGAATAGATTTCAGCACGTCATCAGATTCACAAA

TCGATAAGCCATT

TATTTCAATAGATGAAAACTCGTTCCAAGGCATGTTTAATCTCCAATTTCTAAATATT

CATGATCATTAC

TGGTGGCAACCGAGAGAAACCAGATTGCGTCTACCTAACGGCCTCGTTTACTTGCC

ACGTAAACTCAAAT

GGCTACGGTGGGAAAATTGTCCATTGAAGCGTTTGCCTTCTAATTTTAAGGCTGAGT

ATCTGGTTGAACT

CAGAATGGAGAATAGTGCCCTTGAGAAGCTGTGGAATGGAACTCAGCCTCTTGGAA

GTCTCAAGAAGATG

AATTTGAGGAATTCCAACAATTTGAAAGAAATTCCAGATCTTTCTTTAGCCACAAACC

TCGAGGAATTAG

ATCTTTGTAACTGCGAAGTGCTAGAAAGTTTTCCAAGTCCTCTCAACTCGGAATCTC

TTAAGTTCCTCAA

TCTCCTACTATGCCCCCGGTTGAGAAATTTCCCTGAGATTATAATGCAAAGTTTCAT

CTTTACAGATGAA

ATTGAGATCGAGGTAGCAGATTGTTTATGGAACAAGAATCTCCCTGGACTCGATTAT

CTCGATTGCCTTA

GGAGATGTAATCCAAGTAAATTTCGCCCAGAACATCTCAAAAACCTCACAGTGAGAG

GCAACAACATGCT

TGAGAAGCTATGGGAAGGCGTCCAGTCGCTTGGGAAACTCAAGAGGGTGGATCTG

TCAGAATGTGAAAAC

ATGATAGAAATTCCAGACCTTTCAAAGGCCACCAATCTGGAGATTTTGGATCTCTCA

AATTGCAAAAGTT

TGGTGATGTTACCTTCTACAATTGGGAATCTCCAAAAATTATACACGTTAAATATGGA

AGAATGCACAGG

GCTGAAGGTTCTTCCTATGGATATCAACTTGTCATCTCTCCATACAGTCCATCTCAAA

GGGTGCTCAAGT

TTGAGATTTATCCCTCAGATTTCAAAAAGTATTGCAGTACTCAATCTAGATGACACTG

CCATTGAAGAAG

TTCCATGTTTTGAGAATTTCTCGAGGCTCATGGAATTATCGATGCGTGGTTGCAAGT

CGTTGAGAAGATT

TCCTCAGATTTCAACTAGTATTCAAGAACTCAATCTAGCTGACACCGCCATTGAACA

AGTTCCCTGCTTC

ATTGAGAAATTTTCGAGGCTCAAGGTACTAAATATGAGTGGTTGCAAAATGTTGAAA

AACATATCCCCGA

ACATTTTCAGACTGACAAGGCTTATGAAGGTCGACTTTACAGACTGTGGAGGTGTCA

TCACAGCGTTGAG

TCTTCTATCTAAATTAGACGTCAATGATGTGGAATTTAAGTTTAACGGGACGAGAGT

AAAAAGATGCGGC

ATACGACTCTTGAATGTGTCTACATCTCCGGATGATAGTGAGGGAAGCTCTGAAACA

GAATCTCCGGATG

ATAGTGATGGAGACTCTGTAACAGAGTACCACCAACAGTCTGGAGAAAAATGTGAT

GATGTAGAGACTGA

AAGTAGCAAGAAGCGGATGCGGATGACATTAGGAAACTCTGAAAAATATTTCAACTT

ACCCTGTGGCCAA

ATAGTAACAGACACTGTTCCGTTAGGGTGGGGAGAATCATCATCAGTTTCTTTTAAT

CCATGGCTGGAGG

GGGAAGCTTTGTGTGTTGATTCCATGATTACTGAACAACAAGATGCACAAATTCATA

TAGCTAATGTGGA

TTGGGAGTGGGAGTTATGGTAA

The disease-resistant albumen of＞KO11479 gi|15235930|ref|NP_193427.1| (TIR-NBS-LRR class),

[Arabidopis thaliana] supposed

MASRRYDVFPSFSGVDVRKTFLSHLIEALDRRSINTFMDHGIVRSCIIADEliTAIREARISI

VIFSENY

ASSTWCLNELVEIHKCHKDKDLDQMVIPVFYGVDPSHVRKQIGGFGDVFKKTCEDKPE

DQKQRWVKALTD

ISNLAGEDLRNGPSEAAMVVKIANDVSNKLFPLPKGFGDLVGIEDHIEAIKLKLCLESKEA

RIMVGIWGQ

SGIGKSTIGRALFSQLSSQFHHRAFITYKSTSGSDVSGMKLSWEKELLSEILGQKDIKIEH

FGWEQRLK

HKKVLILLDDVDNLEFLRTLVGKAEWFGSGSRIIVITQDRQLLKAHEIDLIYEVKLPSQGLA

LKMICQYA

FGKYSPPDDFKELAFEVAKLAGNLPLGLSVLGSSLKRRSKEEWMEMLAELQNGLNRDI

MKTLRVSYVRLD

PKDQDIFHYIAWLFNGWKVKSIKDFLGDGVNVNIRLKTLDDKSLIRLTPNDTIEMHNLLQK

LATEIDREE

SNGNPGKRRFLENAEEILDVFTDNTGTEKLLGIDFSTSSDSQIDKPFISIDENSFQGMLNL

QFLNIHDHY

WWQPRETRLRLPNGLVYLPRKLKWLRWENCPLKRLPSNFKAEYLVELRMENSALEKL

WNGTQPLGSLKKM

NLRNSNNLKEIPDLSLATNLEELDLCNCEVLESFPSPLNSESLKFLNLLLCPRLRNFPEIIM

QSFIFTDE

IEIEVADCLWNKNLPGLDYLDCLRRCNPSKFRPEHLKNLTVRGNNMLEKLWEGVQSLG

KLKRVDLSECEN

MIEIPDLSKATNLEILDLSNCKSLVMLPSTIGNLQKLYTLNMEECTGLKVLPMDINLSSLHT

VHLKGCSS

LRFIPQISKSIAVLNLDDTAIEEVPCFENFSRLMELSMRGCKSLRRFPQISTSIQELNLADT

AIEQVPCF

IEKFSRLKVLNMSGCKMLKNISPNIFRLTRLMKVDFTDCGGVITALSLLSKLDVNDVEFKF

NGTRVKRCG

IRLLNVSTSPDDSEGSSETESPDDSDGDSVTEYHQQSGEKCDDVETESSKKRMRMTL

GNSEKYFNLPCGQ

IVTDTVPLGWGESSSVSFNPWLEGEALCVDSMITEQQDAQIHIANVDWEWELW

KO18461 At1g07410

＞KO18461 (gi|7206858) is from the genome sequence of the Arabidopis thaliana BAC F22G5 of karyomit(e) I,

Complete sequence

ATGGCGAATAGAATAGATCATGAGTACGATTACTTGTTCAAGATCGTCCTGATCGGC

GATTCCGGTGTTG

GTAAATCCAACATTCTCTCTCGATTCACCAGAAACGAGTTCTGTCTCGAATCCAAAT

CCACCATTGGCGT

CGAATTCGCCACCCGGACTTTACAGGTCATCTCTCTTCTCTCGCTTTCTCTAAATCT

AGACAATTTCCCT

CCAGATCAATTTGGCAAAACAGTGAAGGCTCAGATTTGGGACACTGCAGGTCAAGA

GCGTTATCGAGCAA

TCACAAGTGCTTACTACAGAGGAGCTGTTGGAGCTCTTCTTGTCTACGACATAACCA

AGAGACAAACTTT

TGAGAATGTCTTGAGATGGTTACGTGAGCTAAGGGATCATGCTGATTCCAACATTGT

TATCATGATGGCT

GGAAACAAATCAGACCTGAATCACTTGAGATCTGTTGCTGATGAAGATGGTCGCTCT

CTCGCCGAGAAGG

AAGGTTTGTCGTTTCTCGAGACATCTGCTTTAGAAGCGACTAACATCGAGAAAGCGT

TTCAGACCATTTT

GTCTGAGATTTATCATATCATAAGCAAGAAAGCTTTAGCGGCACAAGAAGCTGCAGG

TAATCTTCCGGGC

CAAGGAACAGCGATCAATATATCAGATTCATCTGCAACTAACAGAAAAGGATGCTGT

TCTACCTAA

＞KO18461 gi|8778562|gb|AAF79570.1|AC022464_28 F22G5.24[Arabidopis thaliana]

MANRIDHEYDYLFKIVLIGDSGVGKSNILSRFTRNEFCLESKSTIGVEFATRTLQVISLLSL

SLNLDNFP

PDQFGKTVKAQIWDTAGQERYRAITSAYYRGAVGALLVYDITKRQTFENVLRWLRELRD

HADSNIVIMMA

GNKSDLNHLRSVADEDGRSLAEKEGLSFLETSALEATNIEKAFQTILSEIYHIISKKALAAQ

EAAGNLPG

QGTAINISDSSATNRKGCCST

＞BN42015236 gtp binding protein Rab11

atggcgaatagagtggatcaggaatacgattatttgtttaagatcgtgttgatcggagactcgggtgtggggaaatcgaacat

attgtccagattcacgaggaacgagttttgcttggaatccaaatccaccatcggtgtcgaattcgccaccaggactactcagg

tggaaggaaagacgatcaaagctcagatctgggatactgcaggtcaggagaggtacagagctatcactagcgcttactac

cgaggcgcagtgggtgccctccttgctacgacatcaccaagaggcagacctttgacaatgccttgaggtggctccgcgaa

ctcagagaccatgctgattccaacatcgtcatcatgatggctggcaacaaatccgatcttaaccacttgagatccgttgctga

ggaagacggtcacaatctggccgagaaggaaggttctctttcctggagacttctgctctcgaagcaacaaacgtcgaga

aagcctttcagaccatcttaggagagatctaccatatcataagcaaaaaggcactggctgcacaagaagcggctgctgct

aactccgccattccagggcaaggaactaacgattaacgtcgatgacacatctggaggcgtgaaacgaggctgctgctctac

ctaa

＞BN42015236 gtp binding protein Rab11

manrvdqeyylfkivligdsgvgksnilsrftnefclleskstigefatrttqvegktikaqiwdtagqeryraitsayyrgavg

allvyditkrqtfnalrwrelrdhadsnivimmagnksdlnhlrsaeedghnlaekeglsfletsaleatnvekafqtilgeiy

hiiskkalaaqeaaaansaipgqgttinvddtsggvkrgccst*

The gtp binding protein rab11 of＞BN48870948 supposition

atggcgaatcgaatagaccatgagtacgattacttgttcaagatcgtcctcatcggcgactccggtgtcggcaaatccaacat

cctctccagattacccgaaacgagttctgcctcgaatccaaatccaccatcggcgttgaattcgccaccaggactctacag

gttgaaggcaaaacagtgaaggctcagatttgggacacggcagggcaagagcgttaccgagccatcacgagcgcttact

acagaggagccgtcggtgctctcctcgtctacgacatcaccaagagacaaaccttcgagaacgtcctgaggtggctacgc

gagcttagggaccatgccgattccaacattgtgatcatgatggctgggaacaaatcagatctaaaccacctgagatccgttg

ccgacgaagatggtcggtctctagctgagaaggaaggtttgtcgtttctcgagacgtctgctttggaggcgagtaacatcgag

aaagcgtttcagacgattttatctgagatttatcatatcataagcaagaaggcgttggcggcgcaagaagctgcgggtaatct

tcaggttccggggcaaggtaactgccattaacataacggattcgtctgtggctaagagtaaaggatgctgttctacctag

The gtp binding protein rab11 of＞BN48870948 supposition

manridheydylfkivllgdsgvgksnilsrftrnefcleskstigvefatrtlqvegktvkaqlwdtagqeryraitsayyrgavg

allvyitkrqtfenvlrwlrelrdhadsnivimmagnksdlnhlrsvadedgrslaekeglsfletsaleasniekafqtilseiyh

iiskkalaaqeaagnlqvpgqgtainitdssvakskgccst*

＞GM47092542 RAB11C

atggcgcatcgagtggaccacgagtatgactatctgttcaagatcgttttgatcggagactcaggtgtaggaaaatctaacat

cctctccaggttcactcgaaacgagttctgtttagagtccaaatccactatcggagttgagttcgccaccagaactcttcaggt

agagggaaagactgtgaaagcacagatctgggacacagcaggtcaagagcggtaccgtgccattaccagtgcttattac

agaggagctgttggagctctactcgtatatgacataaccaagaggcaaacctttgacaatgtccaaaggtggttgcgtgaac

tgagggaccatgcagactctaatatagttaatcatgattggctggaaataaatctgatttgagccatcttagagcggtttcagagg

atgatggtcaagcattggcagagagggaaggtctctcgtttcttgagacatctgcactggaagcaaccaacattgagaagg

cattccaaaccattttgacagagatttatcatattgttagcaaaaaggcacttgcggctcaggaagcagctgttggtaccaca

cttcctggtcaaggtaccaccatcaatgttggggatgcatctgggaatacaaagagaggctgctgctccacttaa

＞GM47092542 RAB11C

mahrvdheydylfkivligdsgvgksnilsrftmefcleskstigvefatrtlqvegktvkaqiwdtagqeryraitsayyrgavg

allvyditkrqtfdnvqrwlrelrdhadsnivimmagnksdlshlravseddgqalaereglsfletsaleatniekafqtilteiyh

ivskkalaaqeaavgttlpgqgttinvgdasgntkrgccst*

＞GM50564537 RAB11C

atggcgcatcgagtagaccacgagtatgactatctgttcaagatcgttttgatcggagactcaaggtgtaggcaaatccaacat

cctctccaggttcactcgaaacgagttctgtttggagtccaaatccactatcggagttgagttcgccaccagaactcttcaggt

agagggtaaaactgtgaaagcacagatctgggacacagcaggtcaagagcggtaccgtgccattaccagtgcttattaca

gaggagctgttggtgctctacttgtatatgacataaccaagaggcaaacctttgacaatgtccaaaggtggttgcgtgaactg

agggaccatgcggattctaatatagttatcatgatggctggaaataaatctgatttgagccatcttagagcagtttcggaggat

gatggtcaagcattggcagagagggaaggtctctcgtttcttgagacatctgcactggaagcaaccaacattgagaaggca

ttccaaaccattttgacagagatttatcatattgttagcaaaaaggcgctggctgctcaggaagcagctgttggtaccatacttc

ctggtcaaggtaccaccatcaatgttggggatgcatctgggaatacaaagagaggctgctgctccacttaa

＞GM50564537 RAB11C

mahrvdheydylfkivligdsgvgksnilsrftmefcleskstlgvefatrtlqvegktykaqwdtagqeryraitsayyrgavg

allvyditlrqtfdnvqrwlrelrdhadsnivimmagnksdlshlravseddgqalaereglsfletsaleatniekafqtilteiyh

ivskkalaaqeaavgtilpgqgttinvgdasgntkrgccst*

KO28574 At2g20190

＞KO28574 gi|30680912:246-238 Arabidopis thaliana expressed proteins (At2g20190) mRNA,

Complete encoding sequence

ATGGAGGTTTCATCTCCGACGATTATAGTGGAGAGAGCTGGTTCGTATGCTTGGAT

GCATAAGAGTTGGA

GAGTTAGGGAAGAGTTTGCGCGTACTGTTACATCGGCGATTGGTCTTTTCGCATCTA

CGGAACTTCCTCT

TCAGCGTGTTATACTTGCTCCGATACTTCAGATGTTAAATGACCCTAATCAAGCAGT

TAGGGAAGCTGCT

ATTTTGTGCATTGAGGAGATGTATATGCAAGGTGGGTCTCAATTTCGAGAAGAGCTT

CAACGTCACCATC

TTCCATCGTATATGGTGAAGGACATTAATGCTAGACTAGAACGTATTGAGCCACAAC

TGCGTTCTACAGA

TGGCCGTAGTGCCCACCATGTTGTTAATGAGGTGAAGGCATCAAGTGTCAATCCCA

AAAAGAGCAGTCCC

AGGGCAAAGGCTCCTACGAGGGAGAACTCTTTATTTGGGGGAGATGCCGACATCAC

TGAAAAACCCATTG

AGCCAATCAAAGTGTACTCAGAGAAGGAGTTAATACGAGAATTTGAGAAAATTGCTG

CAACACTCGTCCC

AGAGAAAGACTGGTCAATGCGTATTTCAGCTATGCGGAGGGTTGAAGGACTTGTTG

CAGGAGGTGCGACT

GATTACTCCTGCTTTCGAGGTCTCCTGAAGCAACTTGTTGGTCCTTTAAGTACTCAA

TTAGCTGACCGGA

GATCTACCATTGTTAAGCAGGCCTGTCATCTCTTGTGTCTCTTATCAAAAGAGCTAC

TGGGAGATTTTGA

GGCATGCGCTGAGACGTTTATTCCAGTGCTTTTCAAGCTGGTTGTGATTACTGTGCT

TGTAATTGCAGAA

TCTGCTGATAACTGCATAAAAACGATGCTGCGTAACTGCAAAGCTGCCCGTGTACTT

CCTCGCATAGCTG

AATCAGCAAAACATGACCGTAATGCAATTCTGCGAGCAAGATGTTGTGAATATGCAT

TGTTAACACTTGA

ACATTGGCCTGATGCTCCAGAAATTCAACGATCAGTTGATTTATATGAAGATCTGATT

AGATGCTGTGTT

GCAGATGCTATGAGTGAGGTGCGGGCAACTGCTAGAATGTGCTACAGAATGTTTGC

AAAAACTTGGCCGG

ATCGTTCTCGCCGGTTGTTTTCGTCCTTTGACCCTGTCATTCAAAGGCTAATAAATG

AAGAAGATGGTGG

AATTCATAGGAGACACGCCTCACCATCTGTCCGTGAGAGACATTCCCAGCCTTCATT

TTCTCAGACGTCT

GCTCCTTCTAACCTACCTGGCTATGGAACATCAGCTATAGTCGCTATGGATAGAAGT

TCAAATTTATCAT

CTGGAGGATCTCTTTCTTCTGGGTTACTCCTTTCGCAATCAAAGGATGTCAATAAAG

GTTCTGAACGTAG

TCTGGAAAGTGTGTTACAATCAAGCAAGCAGAAGGTCAGTGCAATTGAAAGTATGCT

CCGAGGACTGCAT

ATATCTGATAGACAAAATCCTGCAGCCCTTCGTTCAAGTAGTTTGGATCTAGGAGTT

GACCCTCCATCGT

CTCGTGATCCTCCTTTCCATGCTGTTGCTCCAGCATCCAATAGTCACACAAGTAGCG

CAGCTGCTGAATC

AACACATAGTATCAACAAAGGCAGTAATCGCAATGGTGGCCTTGGTTTGTCAGATAT

CATCACCCAAATT

CAAGCTTCAAAGGACTCAGGAAGATCATCTTACCGTGGCAATCTGTTGTCCGAGTCT

CATCCTACTTTTT

CATCCTTGACCGCTAAACGGGGCTCAGAGAGAAATGAGAGAAGTTCTCTTGAGGAA

AGCAATGATGCCAG

AGAGGTGAGGCGGTTTATGGCTGGTCATTTTGACCGACAGCAGATGGATACTGCTT

ATAGAGATTTGACT

TTCAGGGAATCAAACGCTAGCCATGTTCCCAATTTCCAGAGGCCACTTTTGAGGAA

GAATGTAGGGGGAA

GAATGTCTGCAGGCCGGAGGAGGAGTTTTGATGATAGCCAACTGCAAATTGGTGAC

ATATCAAATTTTGT

TGATGGTCCAGCTTCCCTGAACGAGGCCCTTAACGACGGACTGAACTCAAGTTCTG

ATTGGTGTGCCAGA

GTTGCAGCTTTTAATTTTCTCCAAACTCTGCTGCAGCAAGGCCCAAAAGGTGCTCAA

GAAGTAATTCAAA

GTTTTGAGAAAGTAATGAAACTATTTCTCCGGCATTTGGATGATCCTCACCACAAGG

TCGCACAAGCAGC

ACTGTCGACACTTGCAGATCTTATACCATCTTGCCGAAAGCCTTTTGAGAGCTACAT

GGAAAGAGTCCTA

CCCCATGTGTTTTCACGGCTAATTGACCCTAAAGAAGTAGTTAGACAACCTTGCTCC

TCAACCTTGGAAA

TTGTCAGCAAAACCTACAGTGTGGATTCCCTTTTACCTGCATTGCTTCGTTCACTGG

ATGAACAGAGATC

ACCAAAGGCTAAATTAGCTGTGATTGAATTTGCCATCAACTCCTTCAACAGGTACGC

TGGTAACCCTGAA

ATTTCGGGTAATAGTGGCATCTTAAAGTTGTGGCTGGCAAAGTTGACGCCATTAACC

CGCGACAAAAATA

CCAAGTTGAAAGAAGCTTCCATTACTTGCATCATATCTGTTTACAATCATTATGATTC

TGCGGGACTGCT

AAATTACATTCTTAGTTTGTCGGTTGAGGAGCAAAACTCTCTGAGAAGAGCCCTCAA

ACAATATACTCCC

CGCATCGAGGTGGACCTGTTAAACTATATGCAGAGTAAAAAGGAAAAACAGAGAATT

AAGTCTTATGACC

CATCTGATGCCATTGGGACATCATCTGAGGAAGGATATGCTGGTGCCTCCAAGAAG

AATATATTCCTTGG

CCGGTATTCTGGGGGTTCTATTGACAGTGATAGTGGCAGGAAGTGGAGTTCTTCCC

AGGAGCCAACAATG

ATCACTGGTGGTGTTGGTCAAAATGTTTCCAGTGGAACCCAGGAAAAGCTGTATCA

GAACGTTAGAACTG

GGATCAGTTCAGCTAGTGATCTGTTGAACCCCAAGGATTCTGATTACACATTTGCTT

CAGCTGGTCAGAA

TTCGATATCAAGAACTAGCCCCAATGGAAGCTCAGAAAACATCGAAATCTTGGATGA

CTTATCTCCACCA

CATTTGGAGAAAAATGGTCTAAATCTGACAAGCGTTGATTCCTTGGAAGGAAGACAT

GAAAATGAGGTCT

CCCGCGAATTAGATTTAGGTCACTACATGCTCACATCTATTAAGGTCAACACAACAC

CGGAATCTGGACC

TAGCATTCCTCAGATTCTACATATGATCAACGGGAGTGATGGAAGCCCTTCTTCTAG

CAAGAAATCTGGA

CTCCAGCAATTAATTGAAGCCTCTGTAGCTAACGAGGAATCAGTTTGGACCAAGTAC

TTCAATCAAATTT

TGACGGTTGTTCTTGAAGTGCTCGATGACGAAGATTTTTCAATCAAAGAGCTTGCTC

TTTCATTGATTTC

TGAAATGCTAAAGAGCCAGAAAGATGCCATGGAAGACTCTGTTGAAATAGTGATCG

AAAAGCTGCTTCAT

GTCTCAAAGGACACCGTTCCAAAAGTTTCCACTGAAGCTGAGCAATGTTTGACCACA

GTCTTGTCCCAAT

ACGATCCTTTCAGATGCTTAAGCGTTATTGTCCCATTATTGGTGACGGAAGATGAGA

AAACTCTTGTCGC

TTGCATAAATTGTTTAACGAAGCTTGTGGGTAGGCTCTCGCAAGAGGAATTAATGGA

TCAATTGTCGTCT

TTTTTGCCTGCGGTTTTTGAAGCATTTGGGAGCCAAAGCGCGGATGTCCGCAAGAC

AGTGGTGTTCTGTC

TAGTAGACATATATATAATGCTTGGGAAAGCATTTTTTGCCGTATTTGGAAGGTCTAAA

CAGCACGCAGGT

TCGTCTAGTGACCATCTATGCAAACCGGATCTCGCAGGCTAGAAACGGTGCCCCTA

TCGACGCAGACACC

TGA

＞KO28574 gi|30680g13|ref|NP_849997.1| expressed proteins [Arabidopis thaliana]

MEVSSPTIIVERAGSYAWMHKSWRVREEFARTVTSAIGLFASTELPLQRVILAPILQMLN

DPNQAVREAA

ILCIEEMYMQGGSQFREELQRHHLPSYMVKDINARLERIEPQLRSTDGRSAHHVVNEVK

ASSVNPKKSSP

RAKAPTRENSLFGGDADITEKPIEPIKVYSEKELIREFEKIAATLVPEKDWSMRISAMRRV

EGLVAGGAT

DYSCFRGLLKQLVGPLSTQLADRRSTIVKQACHLLCLLSKELLGDFEACAETFIPVLFKLV

VITVLVIAE

SADNCIKTMLRNCKAARVLPRIAESAKHDRNAILRARCCEYALLTLEHWPDAPEIQRSVD

LYEDLIRCCV

ADAMSEVRATARMCYRMFAKTWPDRSRRLFSSFDPVIQRLINEEDGGIHRRHASPSVR

ERHSQPSFSQTS

APSNLPGYGTSAIVAMDRSSNLSSGGSLSSGLLLSQSKDVNKGSERSLESVLQSSKQK

VSAIESMLRGLH

ISDRQNPAALRSSSLDLGVDPPSSRDPPFHAVAPASNSHTSSAAAESTHSINKGSNRNG

GLGLSDIITQI

QASKDSGRSSYRGNLLSESHPTFSSLTAKRGSERNERSSLEESNDAREVRRFMAGHF

DRQQMDTAYRDLT

FRESNASHVPNFQRPLLRKNVGGRMSAGRRRSFDDSQLQIGDISNFVDGPASLNEALN

DGLNSSSDWCAR

VAAFNFLQTLLQQGPKGAQEVIQSFEKVMKLFLRHLDDPHHKVAQAALSTLADLIPSCR

KPFESYMERVL

PHVFSRLIDPKEVVRQPCSSTLEIVSKTYSVDSLLPALLRSLDEQRSPKAKLAVIEFAINSF

NRYAGNPE

ISGNSGILKLWLAKLTPLTRDKNTKLKEASITCIISVYNHYDSAGLLNYILSLSVEEQNSLR

RALKQYTP

RIEVDLLNYMQSKKEKQRIKSYDPSDAIGTSSEEGYAGASKKNIFLGRYSGGSIDSDSGR

KWSSSQEPTM

ITGGVGQNVSSGTQEKLYQNVRTGISSASDLLNPKDSDYTFASAGQNSISRTSPNGSSE

NIEILDDLSPP

HLEKNGLNLTSVDSLEGRHENEVSRELDLGHYMLTSIKVNTTPESGPSIPQILHMINGSD

GSPSSSKKSG

LQQLIEASVANEESVWTKYFNQILTVVLEVLDDEDFSIKELALSLISEMLKSQKDAMEDSV

EIVIEKLLH

VSKDTVPKVSTEAEQCLTTVLSQYDPFRCLSVIVPLLVTEDEKTLVACINCLKLVGRLS

QEELMDQLSS

FLPAVFEAFGSQSADVRKTVVFCLVDIYIMLGKAFLPYLEGLNSTQVRLVTIYANRISQAR

NGAPIDADT

Claims (35)

1. compare the transformed plant cells of metabolic activity with change with corresponding unconverted wild-type plant cell, wherein metabolic activity by inactivation or downward modulation gene alteration and cause comparing patience and/or the resistance that environment-stress improves with corresponding unconverted wild-type plant cell.

2. the plant transformed cell of claim 1, wherein changed and the relevant metabolic activity of following one or more metabolites, described metabolite is selected from: 2,3-dimethyl-5-leaf chloro chinol, the 2-hydroxy-palmitic acid, 3,4-dopa (=DOPA), the 3-hydroxy-palmitic acid, the 5-oxyproline, L-Ala, alpha-linolenic acid (c18:3 (c9, c12, c15)), alpha-tocopherol, aminoadipic acid, anhydroglucose, arginine, Aspartic Acid, β-A Piaohuluobusuquan, β-Hu Luobusu, β-sitosterol, 5,8-dimethyl tocol, (Δ-7-cis, the 10-cis) hexadecadienoic acid, hiragonic acid, margaric acid, Δ-15-cis nervonic acid, forulic acid, Brassicasterin, cerinic acid (c26:0), citrulline, cryptoxanthin, eicosenoic acid (20:1), fructose, fumaric acid, semi-lactosi, γ-An Jidingsuan, Gamma-Tocopherol, gluconic acid, glucose, L-glutamic acid, glutamine, R-Glyceric acid, Glycerose, glycerine, glycerol-3-phosphate, glycine, homoserine, inositol, Isoleucine, isomaltose, isopentenylpyrophosphate, leucine, Lignoceric acid (c24:0), linolic acid (c18:2 (c9, c12)), luteine, Lyeopene, oxysuccinic acid, seminose, methionine(Met), the methyl furan galactoside, the methyl galactopyranoside, the methyl galactopyranoside, palmitinic acid (c16:0), phenylalanine, phosphoric acid, proline(Pro), putrescine, pyruvic acid, raffinose, ribonic acid, Serine, shikimin, sinapinic acid, stearic acid (c18:0), succsinic acid, sucrose, Threonine, triacontanoic acid, tryptophane, tyrosine, ubiquinone, udp-glucose, Xie Ansuan, zeaxanthin.

3. claim 1 or 2 transformed plant cells, wherein by the one or more inactivations of one or more nucleic acid sequence encodings or the gene alteration metabolic activity of downward modulation, described nucleotide sequence is selected from:

A) nucleic acid molecule of polypeptide shown in encoding sequence Fig. 1 a, 1b, 1c or the 1d;

B) contain the nucleic acid molecule of nucleic acid molecule shown in sequence chart 1a, 1b, 1c or the 1d;

C) nucleic acid molecule, it contains because the nucleotide sequence that the degeneracy of genetic code can be derived from the described peptide sequence of sequence chart 1a, 1b, 1c or 1d;

D) nucleic acid encoding molecule, described polypeptide with (a) have at least 50% identity to the coded amino acid sequence of polypeptide of the nucleic acid molecule of (c), and have the biological activity that sequence chart 1a, 1b, 1c or the described protein of 1d are shown;

E) nucleic acid encoding molecule, described polypeptide separates by the monoclonal antibody of one of the nucleic acid molecule that arrives (d) at (a) coded polypeptide, and has the biological activity that sequence chart 1a, 1b, 1c or the described protein of 1d are shown;

F) can be by the nucleic acid molecule that under stringent hybridization condition, obtains with probe or the suitable nucleic acid library of probe fragment screening, and described nucleic acid molecule encoded polypeptide has the biological activity that protein showed that causes the patience of environment-stress raising when reducing or lack, wherein said probe contains (a) sequence to one of (b) nucleic acid molecule, or described probe fragment has (a) 15nt at least to (c) institute characterisation of nucleic acids molecule, preferred 20nt, 30nt, 50nt, 100nt, 200nt or 500nt

Perhaps described nucleic acid contains and above-mentioned nucleic acid molecule complementary sequence.

4. the transformed plant cells of claim 3, it contains and sequence chart 1a, 1b, the one or more nucleotide sequences of one of the sequence of 1c or 1d homologous, wherein plant is selected from corn, wheat, rye, oat, triticale, rice, barley, soybean, peanut, cotton, Semen Brassicae campestris, rape, cassava, pepper, Sunflower Receptacle, flax, the Borrago officinalis, safflower, Semen Lini, Flower of Beltleaf Primrose, Semen Brassicae campestris, turniprape, Flower of Aztec Marigold, plant of Solanaceae, potato, tobacco, eggplant, tomato, Vicia, pea, clover, coffee, cocoa, tea, Salix, oil palm, coconut, per nnial herb, fodder crop and Arabidopis thaliana.

5. the transformed plant cells of claim 4, the described sequence of its amplifying nucleic acid and sequence chart 1a, 1b, 1c or 1d has the homology at least about 30%.

6. the transformed plant cells of claim 4, the described sequence of its amplifying nucleic acid and sequence chart 1a, 1b, 1c or 1d has the homology at least about 50%.

7. each transformed plant cells among the claim 1-6, wherein environment-stress is selected from salinity, arid, temperature, metal, chemistry, cause of disease and oxidative stress or their combination.

8. each transformed plant cells among the claim 1-7, it is from monocotyledons.

9. each transformed plant cells among the claim 1-7, it is from dicotyledons.

10. each transformed plant cells among the claim 1-9, wherein plant is selected from corn, wheat, rye, oat, triticale, rice, barley, soybean, peanut, cotton, Semen Brassicae campestris, rape, cassava, pepper, Sunflower Receptacle, flax, Borrago officinalis, safflower, Semen Lini, Flower of Beltleaf Primrose, Semen Brassicae campestris, turnip rape, Flower of Aztec Marigold, plant of Solanaceae, potato, tobacco, eggplant, tomato, Vicia, pea, clover, coffee, cocoa, tea, Salix, oil palm, coconut, per nnial herb, fodder crop and Arabidopis thaliana.

11. each transformed plant cells among the claim 1-7, it is from naked cotyledon plant.

12. each transformed plant cells in claim 1-7 or 11, wherein plant is selected from dragon spruce, pine and fir.

13. by the conversion plant that each vegetable cell among the claim 1-10 produces, described plant is unifacial leaf or dicotyledons.

14. the conversion plant of claim 13, described plant are selected from corn, wheat, rye, oat, triticale, rice, barley, soybean, peanut, cotton, Semen Brassicae campestris, rape, cassava, pepper, Sunflower Receptacle, flax, Borrago officinalis, safflower, Semen Lini, Flower of Beltleaf Primrose, Semen Brassicae campestris, turnip rape, Flower of Aztec Marigold, plant of Solanaceae, potato, tobacco, eggplant, tomato, Vicia, pea, clover, coffee, cocoa, tea, Salix, oil palm, coconut, per nnial herb, fodder crop and Arabidopis thaliana.

15. by the conversion plant that each vegetable cell among the claim 1-7,11 or 12 produces, described plant is a gymnosperm.

16. the conversion plant of claim 15, described plant is selected from dragon spruce, pine and fir.

17. the seed that each conversion plant produces among the claim 13-16, wherein seed for inactivation or down the timing gene that causes comparing the patience that environment-stress improves with wild-type plant be hereditary heterozygosis at least.

18. produce the method for comparing the conversion plant of metabolic activity with corresponding unconverted wild-type plant cell with change, described method causes patience and/or the resistance that compares the environment-stress raising with corresponding unconverted wild-type plant by inactivation or down-regulated gene in transforming plant, and this method comprises:

(a) by inactivation or reduce one or more genes and come transformed plant cells, described gene is preferably by one or more nucleic acid encodings of the sequence that is selected from sequence chart 1a, 1b, 1c or 1d and/or its homologue; And

(b) compare the conversion plant that environment-stress has the patience and/or the resistance of raising from this vegetable cell generation with corresponding wild type plant.

19. induce in each the plant among each vegetable cell or the claim 13-16 in claim 1-12 by changing metabolic activity with corresponding unconverted wild-type plant and compare the patience that environment-stress improves and/or the method for resistance, the change of described metabolic activity is to be realized by one or more genes of one or more nucleic acid encodings of the sequence that is selected from sequence chart 1a, 1b, 1c or 1d or its homologue by inactivation or downward modulation.

20. the method for claim 18 or 19, wherein the sequence of the nucleic acid of encoding gene and sequence chart 1a, 1b, 1c or 1d has the homology at least about 30%.

21. the method for claim 20, wherein the sequence of the nucleic acid of encoding gene and sequence chart 1a, 1b, 1c or 1d has the homology at least about 50%.

22. each method among the claim 18-21, the inactivation of wherein said gene or downward modulation by double-stranded RNA interfere (dsRNAi), introduce antisense nucleic acid, ribozyme, with the antisense nucleic acid of ribozyme combination, nucleic acid, the proteinic nucleic acid of coding dominant negative regulation, the described gene of target or RNA or protein DNA binding factor or the RNA binding factor or the protein bound factor that coding suppresses son altogether, induce the RNA degraded viral nucleic acid and expression system, induce system, the sudden change of described gene or the above-mentioned combination of described dna homolog reorganization to realize.

23. contain the expression of plants box of nucleic acid construct, described expression of plants box method by claim 22 when expressing makes one or more gene inactivations or the downward modulation by one or more nucleic acid encodings of the sequence that is selected from sequence chart 1a, 1b, 1c or 1d and/or its homologue and/or its part.

24. detect the method for environment-stress in vegetable cell or the plant, described method comprises that screening compares the metabolic activity that vegetable cell changes with non-stress conditions.

25. the patience that screening vegetable cell or plant are improved environment-stress and/or the method for resistance, described method comprise that screening compares the metabolic activity that vegetable cell changes with non-stress conditions under stress conditions.

26. cultivate patience and/or the vegetable cell of resistance or the method for plant that environment-stress is improved, described method is included in screens the metabolic activity of comparing the vegetable cell change with non-stress conditions under the stress conditions, and selects the vegetable cell that environment-stress is had the patience and/or the resistance of raising.

27. each method among the claim 24-26, wherein changed and the relevant metabolic activity of following one or more metabolites, described metabolite is selected from: 2,3-dimethyl-5-leaf chloro chinol, the 2-hydroxy-palmitic acid, 3,4-dopa (=DOPA), the 3-hydroxy-palmitic acid, the 5-oxyproline, L-Ala, alpha-linolenic acid (c18:3 (c9, c12, c15)), alpha-tocopherol, aminoadipic acid, anhydroglucose, arginine, Aspartic Acid, β-A Piaohuluobusuquan, β-Hu Luobusu, β-sitosterol, 5,8-dimethyl tocol, (Δ-7-cis, the 10-cis) hexadecadienoic acid, hiragonic acid, margaric acid, Δ-15-cis nervonic acid, forulic acid, Brassicasterin, cerinic acid (c26:0), citrulline, cryptoxanthin, eicosenoic acid (20:1), fructose, fumaric acid, semi-lactosi, γ-An Jidingsuan, Gamma-Tocopherol, gluconic acid, glucose, L-glutamic acid, glutamine, R-Glyceric acid, Glycerose, glycerine, glycerol-3-phosphate, glycine, homoserine, inositol, Isoleucine, isomaltose, isopentenylpyrophosphate, leucine, Lignoceric acid (c24:0), linolic acid (c18:2 (c9, c12)), luteine, Lyeopene, oxysuccinic acid, seminose, methionine(Met), the methyl furan galactoside, the methyl galactopyranoside, the methyl galactopyranoside, palmitinic acid (c16:0), phenylalanine, phosphoric acid, proline(Pro), putrescine, pyruvic acid, raffinose, ribonic acid, Serine, shikimin, sinapinic acid, stearic acid (c18:0), succsinic acid, sucrose, Threonine, triacontanoic acid, tryptophane, tyrosine, ubiquinone, udp-glucose, Xie Ansuan, zeaxanthin.

28. each method among the claim 25-27, wherein the metabolic activity of Gai Bianing is because the gene of one or more inactivations or downward modulation.

29. each method among the claim 25-28, wherein inactivation or the downward modulation by one or more genes changes metabolic activity, and described gene is by the one or more nucleic acid sequence encodings that are selected from nucleotide sequence shown in sequence chart 1a, 1b, 1c or the 1d and/or its homologue.

30. contain the transformed plant cells of the gene of inactivation or downward modulation, described gene is coded by the nucleotide sequence of the sequence that is selected from sequence chart 1a, 1b, 1c or 1d and/or its homologue.

31. contain the isolated nucleic acid molecule that is selected from following nucleic acid molecule:

A) coding contains the nucleic acid molecule of the polypeptide of polypeptide shown in sequence chart 1a, 1b, 1c or the 1d;

B) contain the nucleic acid molecule of polynucleotide shown in sequence chart 1a, 1b, 1c or the 1d;

C) nucleic acid molecule, it contains because the nucleotide sequence that the degeneracy of genetic code can be derived from (b) described peptide sequence, and described polypeptide has the biological activity that protein showed of sequence chart 1a, 1b, 1c or 1d;

D) nucleic acid encoding molecule, described polypeptide with (a) or the coded amino acid sequence of polypeptide of nucleic acid molecule (c) have at least 50% identity, and have the biological activity that protein showed of sequence chart 1a, 1b, 1c or 1d;

E) nucleic acid encoding molecule, described polypeptide separates by the monoclonal antibody of one of the nucleic acid molecule that arrives (d) at (a) coded polypeptide, and has the activity that protein X is shown;

F) can be by the nucleic acid molecule that under stringent hybridization condition, obtains with probe or the suitable nucleic acid library of probe fragment screening, and described nucleic acid molecule encoded polypeptide has the activity that protein X is shown, wherein said probe contains (a) sequence to one of (b) nucleic acid molecule, or described probe fragment has (a) 15nt at least to (i) institute characterisation of nucleic acids molecule, preferred 20nt, 30nt, 50nt, 100nt, 200nt or 500nt;

G) nucleic acid molecule that has at least 70% sequence identity with the polynucleotide that are selected from polynucleotide shown in sequence chart 1a, 1b, 1c or the 1d;

Perhaps described nucleic acid contains and above-mentioned nucleic acid molecule complementary sequence; Nucleic acid molecule according to (a) to (g) is different from the described sequence of sequence chart 1a, 1b, 1c or 1d at least on one or more Nucleotide thus, and coding is different from the protein of sequence chart 1a, 1b, 1c or the described protein sequence of 1d at least on one or more amino acid.

32. by the coded isolated polypeptide of the nucleic acid molecule of claim 31.

33. polypeptid specificity bonded antibody with claim 32.

34. the plant transformed cell, wherein patience that environment-stress is improved and/or resistance are provided by the gene of one or more inactivations or downward modulation, and described gene is selected from following nucleic acid sequence encoding by one or more:

A) nucleic acid molecule of polypeptide shown in encoding sequence Fig. 1 a, 1b, 1c or the 1d;

B) contain the nucleic acid molecule of nucleic acid molecule shown in sequence chart 1a, 1b, 1c or the 1d;

C) nucleic acid molecule, it contains because the nucleotide sequence that the degeneracy of genetic code can be derived from the described peptide sequence of sequence chart 1a, 1b, 1c or 1d;

D) nucleic acid encoding molecule, described polypeptide with (a) have at least 50% identity to the coded amino acid sequence of polypeptide of the nucleic acid molecule of (c), and have the biological activity that sequence chart 1a, 1b, 1c or the described protein of 1d are shown;

E) nucleic acid encoding molecule, described polypeptide separates by the monoclonal antibody of one of the nucleic acid molecule that arrives (d) at (a) coded polypeptide, and has the biological activity that sequence chart 1a, 1b, 1c or the described protein of 1d are shown;

A) can be by the nucleic acid molecule that under stringent hybridization condition, obtains with probe or the suitable nucleic acid library of probe fragment screening, and described nucleic acid molecule encoded polypeptide has the biological activity that protein showed that causes the patience of environment-stress raising when reducing or lack, wherein said probe contains (a) sequence to one of (b) nucleic acid molecule, or described probe fragment has (a) 15nt at least to (c) institute characterisation of nucleic acids molecule, preferred 20nt, 30nt, 50nt, 100nt, 200nt or 500nt; With

G) nucleic acid molecule that has at least 70% sequence identity with the polynucleotide that are selected from polynucleotide shown in sequence chart 1a, 1b, 1c or the 1d;

Perhaps described nucleic acid contains and above-mentioned nucleic acid molecule complementary sequence;

Or contain nucleic acid molecule with its complementary sequence.

35. contain the plant of the cell of claim 34.