CN1807583A - Method for cultivating phaffiafhodozyma enriched with astaxanthin using waste water from bean curd production - Google Patents
Method for cultivating phaffiafhodozyma enriched with astaxanthin using waste water from bean curd production Download PDFInfo
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- CN1807583A CN1807583A CN 200610045614 CN200610045614A CN1807583A CN 1807583 A CN1807583 A CN 1807583A CN 200610045614 CN200610045614 CN 200610045614 CN 200610045614 A CN200610045614 A CN 200610045614A CN 1807583 A CN1807583 A CN 1807583A
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- Prior art keywords
- astaxanthin
- phaffiafhodozyma
- cultivate
- yellow seriflux
- shrimpanin
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- 238000000034 method Methods 0.000 title claims abstract description 10
- JEBFVOLFMLUKLF-IFPLVEIFSA-N Astaxanthin Natural products CC(=C/C=C/C(=C/C=C/C1=C(C)C(=O)C(O)CC1(C)C)/C)C=CC=C(/C)C=CC=C(/C)C=CC2=C(C)C(=O)C(O)CC2(C)C JEBFVOLFMLUKLF-IFPLVEIFSA-N 0.000 title claims description 44
- MQZIGYBFDRPAKN-ZWAPEEGVSA-N astaxanthin Chemical compound C([C@H](O)C(=O)C=1C)C(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)C(=O)[C@@H](O)CC1(C)C MQZIGYBFDRPAKN-ZWAPEEGVSA-N 0.000 title claims description 44
- 229940022405 astaxanthin Drugs 0.000 title claims description 44
- 235000013793 astaxanthin Nutrition 0.000 title claims description 44
- 239000001168 astaxanthin Substances 0.000 title claims description 44
- 238000004519 manufacturing process Methods 0.000 title claims description 13
- 239000002351 wastewater Substances 0.000 title abstract description 6
- 235000013527 bean curd Nutrition 0.000 title 1
- 210000005253 yeast cell Anatomy 0.000 claims abstract description 13
- 210000004027 cell Anatomy 0.000 claims abstract description 9
- 239000002994 raw material Substances 0.000 claims abstract description 8
- 230000001954 sterilising effect Effects 0.000 claims abstract description 8
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 8
- 239000007788 liquid Substances 0.000 claims description 18
- 238000000855 fermentation Methods 0.000 claims description 10
- 230000004151 fermentation Effects 0.000 claims description 10
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 6
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 6
- 230000001580 bacterial effect Effects 0.000 claims description 6
- 102000004169 proteins and genes Human genes 0.000 claims description 6
- 108090000623 proteins and genes Proteins 0.000 claims description 6
- 238000007796 conventional method Methods 0.000 claims description 5
- 238000000605 extraction Methods 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 5
- 238000004321 preservation Methods 0.000 claims description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 4
- 239000008103 glucose Substances 0.000 claims description 4
- 238000003811 acetone extraction Methods 0.000 claims description 2
- 238000011081 inoculation Methods 0.000 claims description 2
- 235000016709 nutrition Nutrition 0.000 claims description 2
- 230000035764 nutrition Effects 0.000 claims description 2
- 238000009423 ventilation Methods 0.000 claims description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 abstract 2
- 210000002421 cell wall Anatomy 0.000 abstract 2
- 238000012258 culturing Methods 0.000 abstract 2
- 239000000654 additive Substances 0.000 abstract 1
- 230000000996 additive effect Effects 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 230000003313 weakening effect Effects 0.000 abstract 1
- 239000002028 Biomass Substances 0.000 description 4
- 210000000130 stem cell Anatomy 0.000 description 3
- 235000021466 carotenoid Nutrition 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- 230000001965 increasing effect Effects 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000081271 Phaffia rhodozyma Species 0.000 description 1
- LPQOADBMXVRBNX-UHFFFAOYSA-N ac1ldcw0 Chemical compound Cl.C1CN(C)CCN1C1=C(F)C=C2C(=O)C(C(O)=O)=CN3CCSC1=C32 LPQOADBMXVRBNX-UHFFFAOYSA-N 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000003674 animal food additive Substances 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 229940088623 biologically active substance Drugs 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 150000001747 carotenoids Chemical class 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- -1 keto-acid carotenoid Chemical class 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
A method for culturing shrimpanin-rich red-hair yeast with yellow pulp-water. Use yellow pulp-water as raw material; add cell wall weakening agent; inoculate red-hair yeast after sterilization; ferment for some time; collect the shrimpanin-rich red-hair yeast cell. Also we can furtherly do cell wall breaking treatment and extrat shrimpanin from the cell with acetone. The red-hair yeast achieved has high biogauge and yield much shrimpanin per volume waste water. Its advantanges contain: investment little; raw materials cost little; reduce the evironment pollution and resourse wasting because of waste water exclution. Both the achieved red-hair yeast and the shrimpanin extracted from it can be used as feedingstuff additive for top-grage aquatic products and birds, and can make the culturing products have high commondity value.
Description
Technical field
The present invention relates to the preparation field of biologically active substance, particularly a kind of method of utilizing yeast fermentation to prepare natural astaxanthin.
Background technology
Can produce a large amount of waste water in the bean product course of processing, it mainly is yellow seriflux, total amount is the heavy 5.5-7 of beans times, contains nutritive ingredients such as monose, VITAMIN, organic acid, water-soluble protein, amino acid, lipid in the water, belongs to the high waste water of high density biodegradability degree.At present a large amount of yellow plasm of soybean is used as discharge of wastewater, has not only wasted resource but also contaminate environment, has also increased the load of environmental improvement.Astaxanthin is a kind of keto-acid carotenoid with high economic worth, extensively is present in the organic sphere, particularly in aquatic animal such as shrimp, crab, fish and the plumage.Because astaxanthin has unique colouring function, extremely strong oxidation-resistance, on anticancer change, enhancing immunity and anti-biological function such as photosensitive, far be better than other carotenoid, thereby have broad application prospects in feed, food, makeup and medicine and other fields.The main source of current astaxanthin is to extract from the fishery products waste, utilizes algae and yeast culture production.Phaffiafhodozyma (Phaffia rhodozyma) is considered to one of bacterial classification of realizing the tool potentiality of fermentation method suitability for industrialized production natural astaxanthin.Compare with other sophisticated tunning, utilize the scale of microbiological industry production astaxanthin to differ greatly, subject matter is still and yields poorly, the fermentation costs height.For reducing production costs, the research worker is seeking cheap substrate as a research focus both at home and abroad.
Summary of the invention
Purpose of the present invention is intended to utilize a large amount of yellow serifluxes that produce in the bean product course of processing, produces natural astaxanthin, makes full use of resource to reach, and reduces environmental pollution, and reduces the purpose of astaxanthin fermentation production cost.
The technical solution adopted for the present invention to solve the technical problems is: a kind of method of utilizing yellow seriflux to cultivate the phaffiafhodozyma that is rich in astaxanthin is provided, it is characterized in that: utilize the discarded yellow seriflux that produces in the production process of bean products to be basic material, add or do not add the exogenous nutrition material, after sterilization and inoculation, cultivate by the liquid ventilation, the phaffiafhodozyma of astaxanthin is rich in acquisition, and then this yeast cell passed through broken wall treatment, astaxanthin with in the acetone extraction cell comprises the steps:
The processing of a, raw material: interpolation 0-50 gram glucose in yellow seriflux/rise yellow seriflux, be heated to 115-125 ℃ of sterilization 20min then, standby;
B, liquid seeds cultivate: the slant preservation bacterial classification is activated, ventilates and cultivate 24h in cultivating 22 ℃ of liquid in the YM substratum in 22 ℃ on the wort slant medium of 10Bx;
C, phaffiafhodozyma ventilating fermentation: with the yellow seriflux volumeter, in the fermention medium yellow seriflux, inoculate the phaffiafhodozyma seed liquor of 4-16% (volume), ventilate in 22 ℃ of liquid and cultivate phaffiafhodozyma 48-96h; Collect yeast cell.
D, extraction astaxanthin: measure the content astaxanthin in the yeast cell, extract astaxanthin then according to a conventional method, and then fox extracting thallus protein.
In yellow seriflux, suitably add nutritive substances such as glucose, help the growth of phaffiafhodozyma cell, and then can improve the output of astaxanthin by increasing the output of phaffiafhodozyma cell.
The inventive method is a basic material with the discarded yellow seriflux that produces in the production process of bean products, the phaffiafhodozyma cell biomass that obtains is higher, the astaxanthin yield height, tropina after therefrom astaxanthin of Ti Quing or astaxanthin extract all can be used for the breeding feed additive of high-grade aquatic products and bird etc., can improve the commodity value of fishery products or birds, beasts and eggs etc. greatly.Advantages such as employing the inventive method is produced natural astaxanthin, not only utilizes resource fully, reduces environmental pollution, and has reduced the astaxanthin fermentation production cost significantly, has less investment, and raw materials cost is low have very high utilization and extention and are worth.
The invention will be further described below in conjunction with embodiment and Figure of description.
Description of drawings
Fig. 1 utilizes yellow seriflux to cultivate the process flow sheet of the phaffiafhodozyma that is rich in astaxanthin.
Embodiment
Embodiment 1.
The processing of a, raw material: yellow seriflux is heated to 115 ℃ of sterilization 20min, standby;
B, liquid seeds cultivate: the slant preservation bacterial classification is activated, ventilates and cultivate 24h in cultivating 22 ℃ of liquid in the YM substratum in 22 ℃ on the wort slant medium of 10Bx;
C, phaffiafhodozyma ventilating fermentation: with the yellow seriflux volumeter, in the fermention medium yellow seriflux, inoculate the phaffiafhodozyma seed liquor of 8% (volume), ventilate in 22 ℃ of liquid and cultivate phaffiafhodozyma 72h; Collect yeast cell.
D, extraction astaxanthin: measure the content astaxanthin in the yeast cell, extract astaxanthin then according to a conventional method, and then fox extracting thallus protein.
The acquisition biomass is 4.74g/L, astaxanthin volumetric production 2.43mg/L, cell content astaxanthin 512ug/g stem cell.
Embodiment 2.
The processing of a, raw material: in yellow seriflux, add 10,20,30,40 respectively, 50g glucose/L rises yellow seriflux, is heated to 120 ℃ of sterilization 20min then, and is standby;
B, liquid seeds cultivate: the slant preservation bacterial classification is activated, ventilates and cultivate 24h in cultivating 22 ℃ of liquid in the YM substratum in 22 ℃ on the wort slant medium of 10Bx;
C, phaffiafhodozyma ventilating fermentation: with the yellow seriflux volumeter, in the fermention medium yellow seriflux, inoculate the phaffiafhodozyma seed liquor of 4% (volume), ventilate in 22 ℃ of liquid and cultivate phaffiafhodozyma 96h; Collect yeast cell.
D, extraction astaxanthin: measure the content astaxanthin in the yeast cell, extract astaxanthin then according to a conventional method, and then fox extracting thallus protein.
Obtain that biomass is respectively 8.41,10.67,13.22,14.53,15.20g/L, the astaxanthin volumetric production is respectively 4.21,4.83,5.55,6.20, the 6.73mg/L fermented liquid, and the cell content astaxanthin is respectively 501,453,420,428, the 442ug/g stem cell.
Embodiment 3.
The processing of a, raw material: yellow seriflux is heated to 125 ℃ of sterilization 20min, standby;
B, liquid seeds cultivate: the slant preservation bacterial classification is activated, ventilates and cultivate 24h in cultivating 22 ℃ of liquid in the YM substratum in 22 ℃ on the wort slant medium of 10Bx;
C, phaffiafhodozyma ventilating fermentation: with the yellow seriflux volumeter, in the fermention medium yellow seriflux, inoculate the phaffiafhodozyma seed liquor of 16% (volume), ventilate in 22 ℃ of liquid and cultivate phaffiafhodozyma 48h; Collect yeast cell.
D, extraction astaxanthin: measure the content astaxanthin in the yeast cell, extract astaxanthin then according to a conventional method, and then fox extracting thallus protein.
The acquisition biomass is 4.68g/L, astaxanthin volumetric production 3.58mg/L, cell content astaxanthin 485ug/g stem cell.
Claims (1)
1, a kind of method of utilizing yellow seriflux to cultivate the phaffiafhodozyma that is rich in astaxanthin, it is characterized in that: utilize the discarded yellow seriflux that produces in the production process of bean products to be basic material, add or do not add the exogenous nutrition material, after sterilization and inoculation, cultivate by the liquid ventilation, obtain to be rich in the phaffiafhodozyma of astaxanthin, and then this yeast cell is passed through broken wall treatment, astaxanthin with in the acetone extraction cell comprises the steps:
The processing of a, raw material: interpolation 0-50 gram glucose in yellow seriflux/rise yellow seriflux, be heated to 115-125 ℃ of sterilization 20min then, standby;
B, liquid seeds cultivate: the slant preservation bacterial classification is activated, ventilates and cultivate 24h in cultivating 22 ℃ of liquid in the YM substratum in 22 ℃ on the wort slant medium of 10Bx;
C, phaffiafhodozyma ventilating fermentation: with the yellow seriflux volumeter, in the fermention medium yellow seriflux, inoculate the phaffiafhodozyma seed liquor of 4-16% (volume), ventilate in 22 ℃ of liquid and cultivate phaffiafhodozyma 48-96h; Collect yeast cell;
D, extraction astaxanthin: measure the content astaxanthin in the yeast cell, extract astaxanthin then according to a conventional method, and then fox extracting thallus protein.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100456147A CN100386426C (en) | 2006-01-05 | 2006-01-05 | Method for cultivating phaffiafhodozyma enriched with astaxanthin using waste water from bean curd production |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100456147A CN100386426C (en) | 2006-01-05 | 2006-01-05 | Method for cultivating phaffiafhodozyma enriched with astaxanthin using waste water from bean curd production |
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| Publication Number | Publication Date |
|---|---|
| CN1807583A true CN1807583A (en) | 2006-07-26 |
| CN100386426C CN100386426C (en) | 2008-05-07 |
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| CNB2006100456147A Expired - Fee Related CN100386426C (en) | 2006-01-05 | 2006-01-05 | Method for cultivating phaffiafhodozyma enriched with astaxanthin using waste water from bean curd production |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101579040B (en) * | 2009-06-14 | 2011-10-12 | 中国热带农业科学院热带作物品种资源研究所 | Preparation method of astaxanthin biological feed |
| CN105087737A (en) * | 2015-08-19 | 2015-11-25 | 华南理工大学 | Method for preparing astaxanthin by fermenting Jerusalem artichoke and application thereof |
| CN108660176A (en) * | 2018-05-03 | 2018-10-16 | 荆楚理工学院 | A method of culture scenedesmus obliquus algae produces natural carotenoid |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2203315B1 (en) * | 2002-02-04 | 2005-06-16 | Antibioticos, S.A.U. | ASTAXANTINE PRODUCTION PROCEDURE THROUGH THE FERMENTATION OF SELECTED VINTAGES OF XANTHOPHYLLOMYCES DENDRORHOUS. |
| EP1543143A2 (en) * | 2002-09-27 | 2005-06-22 | DSM IP Assets B.V. | Astaxanthin production using fed-batch fermentation process by phaffia rhodozyma |
| CN1225546C (en) * | 2003-07-23 | 2005-11-02 | 天津大学 | Method for preparing astaxanthin from alga cultivated by using residue of fermenting yeast |
| CN1282749C (en) * | 2003-11-25 | 2006-11-01 | 华南理工大学 | Course feeding fermentation method for preparing astaxanthin with molasses or starch sugar as materials |
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2006
- 2006-01-05 CN CNB2006100456147A patent/CN100386426C/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101579040B (en) * | 2009-06-14 | 2011-10-12 | 中国热带农业科学院热带作物品种资源研究所 | Preparation method of astaxanthin biological feed |
| CN105087737A (en) * | 2015-08-19 | 2015-11-25 | 华南理工大学 | Method for preparing astaxanthin by fermenting Jerusalem artichoke and application thereof |
| CN105087737B (en) * | 2015-08-19 | 2018-07-20 | 华南理工大学 | It is a kind of to utilize the method and application that jerusalem artichoke is fermenting raw materials production astaxanthin |
| CN108660176A (en) * | 2018-05-03 | 2018-10-16 | 荆楚理工学院 | A method of culture scenedesmus obliquus algae produces natural carotenoid |
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| CN100386426C (en) | 2008-05-07 |
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