CN108660176A - A method of culture scenedesmus obliquus algae produces natural carotenoid - Google Patents
A method of culture scenedesmus obliquus algae produces natural carotenoid Download PDFInfo
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Abstract
The present invention provides a kind of culture scenedesmus obliquus newly to tame the method that algae Scenedesmus obliquus New produce natural carotenoid, by yellow serofluid pretreatment, adjust pH, addition selenium-rich culturing object (bestowing favour from " world's selenium is all "), sterilizing, both sides feux rouges fluorescent tube laterally to optical culture, by methyl jasmonate and top to blue light 7600lux, 21h of light：3h light dark periods, 32 37 DEG C of thermal stimulus, nitrogen hunger, glucose and xylose inducing carotenoid prepare selenium-rich carotenoid dry product using frond cell cryogenic high pressure homogeneous broken wall or ultrasonic wave assisted extraction carotenoid.Every gram of frond dry weight of the present invention can obtain novel selenium-enriched natural carotenoid Scenecarotenoid dry product 123.12mg, and this method has notable help, great development prospect and market value to production selenium natural carotenoid new product.
Description
Technical field
The present invention relates to Extraction of carotenoid pigment fields, and natural class is produced more particularly, to a kind of culture scenedesmus obliquus algae
The method of carrotene.
Background technology
Carotenoid is proved to be one of high-grade nutrient product and drug, is safeguarding bone and dental health, is preventing sclerotin
Osteoporosis, prevention and treatment cataract and macular degeneration, prevention diabetes prevent Alzheimer's disease and prevent cardiovascular and cerebrovascular
Disease has healthcare function outstanding (such as Wu Qingying Chinese foods and nutrition, 2016,22 (4):32-34), it is that cancer is pre-
Anti- dose, ulcer inhibitor and life extension agent.However, human body itself can not synthesize carotenoid, mainly from vegetables and fruit
Middle intake, carotenoid in selling market based on chemical synthesis, far from meeting the needs of modern consumer, and
And the carotenoid that current most of enterprise uses is the chemically synthesized product containing cause gene mutation action component mostly, and
The problem of this respect (China's leap etc., the patent No. 201210001376) is then not present in natural carotenoid.Therefore, it chemically closes
It is a kind of ideal selection at switching to newly tame extraction in algae from scenedesmus obliquus.
Yellow plasm of soybean is generated waste water in bean product process, and yellow plasm of soybean contains vitamin B2, dimension life
The functional components such as plain B12, carbohydrate, soyabean oligosaccharides, isoflavones and soybean whey protein, total sugar content is about
For 0.6-0.7% or so.Carotenoid is produced with yellow plasm of soybean, extends industrial chain, realizing turns waste into wealth, and reduces
Environmental pollution (Li Yunjie etc., science and technology and innovation, 2016,5：9-10), it realizes recycling for resource, and creates certain
Social and economic benefit has important realistic meaning to the good life for realizing yearned for.
Selenium is " long-lived element ", " natural antidote " etc. by good reputation, and China is selenium deficiency big country, and the crowd of selenium deficiency population 400,000,000 lacks
Selenium is related with more than 40 kinds of diseases.Selenium is a kind of biological anti-oxidant, it is likely in the metabolism of scenedesmus obliquus as life
The long factor participates in the positive of carotenogenesis related enzyme systems and adjusts, and when debita spissitudo promotes the growth of frustule and secondary generation
Thank to the synthesis of product.The ingredients such as the astaxanthin in appropriate selenium and natural carotenoid can act synergistically, therefore have high warp
Ji value.And selenium-rich natural carotenoid has important and extensive medicine health care prevention function, as can anti-hypertension, resisting
Diabetes, lipidemia, cancer-resisting, anti-fatty liver, anti-senile dementia disease, anti-inflammatory antiulcer, anti-aging and promote longevity
Deng.
Methyl jasmonate can induce the physiological reaction of many plants as a kind of signaling molecule in the sources Inner, regulate and control plant
Growth and development, resistance reaction and secondary metabolite synthesis.
Scenedesmus obliquus newly tames algae (Scenedesmus obliquus New) and is very easy to large-scale production, is one
The selenium natural carotenoid biological source of kind great potential, this seeds algae have one of the main reason for oxidation resistance outstanding
It is it to synthesize comprising the secondary carotenoid including distinctive astaxanthin and content accumulation height, frond is likely via secondary
The mobility of raw carotenoid efficiently supports frustule to grow.Developing and using selenium-rich natural carotenoid has important quotient
Industry application value.It solves the problems, such as that selenium-supply can expand consumer groups, allows more consumers to enjoy selenium natural carotenoid strong
Benefit caused by big inoxidizability.
Invention content
It is an object of the present invention to provide a kind of scenedesmus obliquus newly to tame algae (Scenedesmus obliquus New) production
The method of natural carotenoid, this method be for a large amount of yellow plasm of soybean as waste be discharged and caused by it is a large amount of
Waste and serious pollution (multifarious extremely etc., the patent No. 201310660674) and the peculiar high anti-oxidation ability of novel scenedesmus obliquus
（Especially Scenecarotenoid）Utilization, it is simplified that a kind of solution yellow serofluid for providing administers problem, technique
The method for producing natural carotenoid new product using yellow serofluid culture scenedesmus obliquus, algae used are tamed and dociled for this laboratory selection and breeding
Change, derives from Jingchu Biological Engineering College of the Institute of Technology.The method of the present invention includes the following steps：
S1, yellow serofluid pretreatment：After yellow serofluid in bean product manufacturing process is collected, using 5-7 layers of filtered through gauze, after filtering
The pH values of yellow serofluid filtrate are adjusted to 7.5-8.0；
S2, selenium-rich yellow serofluid culture medium is produced：The richness that mass volume ratio is 8%-12% is added in the yellow serofluid filtrate made from S1
Selenium potato juice and the concentration selenium-rich spring that mass volume ratio is 25%-35%, yellow serofluid filtrate, selenium-rich potato juice and concentration are rich
The mass ratio of selenium spring is 7:2:1,121 DEG C, the sterilizing of 30min autoclavings are then used, selenium-rich yellow serofluid training is made
Support base；
S3, activation scenedesmus obliquus algae：Scenedesmus obliquus algae is activated with the selenium-rich yellow serofluid medium culture obtained in S2；
S4, algae are tentatively cultivated：Scenedesmus obliquus algae after being activated in selenium-rich yellow serofluid inoculation of medium S3, initial inoculation
Density is 0.95 × 106A/mL is irradiated using feux rouges fluorescent tube and is cultivated, and methyl jasmonate is added in intensity 1500-2000lux,
25 DEG C of static gas wave refrigerators, up to scenedesmus obliquus culture to exponential phase；
S5, algae Fiber differentiation：The cultivation temperature of the scenedesmus obliquus of exponential phase is increased to 32-37 DEG C, in blue light tube
Under irradiation, improves intensity of illumination and carry out Fiber differentiation, light dark period 21h to 7500-7700lux：It is trained in the culturing rack of 3h
It supports, adding in glucose and xylose to culture medium makes glucose and xylose concentration respectively reach 8-12g/L and 27.0-37.0g/L,
Concussion shaking flask is primary daily, cultivates 15-20 days；
S6, chlorophyll and frond breaking-wall cell are destroyed：It takes out algae solution centrifugation in S5 and removes supernatant, collect frond precipitation, it is heavy in frond
The methanol solution that KOH solution and 18%-20% that mass fraction is 2%-4% are added in shallow lake destroys chlorophyll 8-10min, is collected by centrifugation
Precipitation, is dissolved in aqua sterilisa, then carries out cryogenic high pressure homogeneous broken wall treatment or supersonic wave wall breaking handles to obtain the algae after broken wall
Body cell；
S7, carotenoid extraction：It is 75%-90%'s that the mass fraction that ice bath is crossed will be added in frond cell after broken wall in S6
Ethanol solution carries out Extraction of carotenoid pigment, solid-liquid ratio 1:4.2, solid-liquid ratio unit is mg/mL, and extraction time is ice bath 40-
50min, repeats extraction time 2-3 times, until frond bleaches, filters and collects Extraction of carotenoid pigment liquid；
It is prepared by S8, carotenoid dry product：By the Extraction of carotenoid pigment liquid in S7 by boiling off solvent, concentration, crystallization, vacuum are done
Carotenoid dry product is obtained after dry.
Scenedesmus obliquus algae used is this laboratory selection and breeding domestication, derives from Jingchu Biological Engineering College of the Institute of Technology, tiltedly
The astaxanthin-containing of raw scenedesmus algae is 0.09%, is cultivated by the method for ultraviolet radioactive and nitrosoguanidine Combined Stress.
The selenium content of selenium-rich potato juice is 1.75-1.95mg/kg, and concentration selenium-rich spring selenium content is 20-24mg/L.
Feux rouges fluorescent tube is the left and right sides to light, and the distance between both sides feux rouges fluorescent tube and algae solution are 12-14cm, selenium-rich Huang
Methyl jasmonate concentration is increased to 5.25mg/mL in 3 days before exponential phase by 3.5mg/mL in pulp-water culture medium.
Blue light tube is top to light, and the distance between top and algae solution are 32-34cm, stress-inducing carotenoid.
The condition of cryogenic high pressure homogeneous broken wall is:9 DEG C, 70MPa, solid-liquid ratio 1:5.5, solid-liquid ratio unit is mg/mL,
Cycle 2-3 times.
Present invention utilizes bean product yellow serofluid culture scenedesmus obliquus newly to tame algae (Scenedesmus obliquus
New natural carotenoid substance) is produced, a kind of new purposes is opened for comprehensive utilization bean product yellow serofluid, turns waste into wealth,
First production selenium-rich natural carotenoid new product is used for (health care）It is used in food and feed additive, to improve bean product
Business Economic Benefit.
The beneficial effects of the invention are as follows：（1）Appropriate selenium accelerates frond growth, and frond breeding is ultrafast, and every gram of frond dry weight is most
After can obtain 123.12 mg of new type natural carotenoid Scenecarotenoid dry products, take full advantage of scenedesmus obliquus and newly tame
Algae (Scenedesmus obliquus New) produces the feature efficient, with short production cycle of carotenoid, to realizing work
The production of industry metaplasia has apparent help.（2）Selenium-rich Extraction of carotenoid pigment rate is high in frond cell, and product does not have toxic residue, safety
Property it is high, under the premise of mending Organic Selenium and becoming new fashion, great development prospect and market value.
Description of the drawings
Fig. 1 is the process flow diagram of the method for the present invention.
Specific implementation mode
Below with reference to shown in attached drawing and embodiment invention is further described in detail, the present invention in " mass body
Product ratio " refers to that the quality of selenium-rich potato juice and concentration selenium-rich spring accounts for ratio with yellow serofluid filtrate volume, wherein working as selenium-rich
When the quality of potato juice and concentration selenium-rich spring is using " g " as unit, the unit of corresponding volume is " L ".
Embodiment 1
By taking JN-6001 type cryogenic high pressure homogenizers carry out cryogenic high pressure homogeneous broken wall and ethyl alcohol extraction as an example
A kind of method of scenedesmus obliquus algae production natural selenium-rich natural carotenoid of the present invention, includes the following steps：S1、
Yellow serofluid pre-processes：After yellow serofluid in bean product manufacturing process is collected, using 5-7 layers of filtered through gauze, used after filtering
NaOH adjusts the pH values of yellow serofluid filtrate to 7.5-8.0；
S2, selenium-rich yellow serofluid culture medium is produced：The richness that mass volume ratio is 8%-12% is added in the yellow serofluid filtrate made from S1
Selenium potato juice (bestowing favour from " world's selenium is all ") and the concentration selenium-rich spring that mass volume ratio is 25%-35% (come from " world's selenium
All " bestow favour), the mass ratio of yellow serofluid filtrate, selenium-rich potato juice and concentration selenium-rich spring is 7:2:1, then use 121 DEG C,
30min autoclavings sterilize, and selenium-rich yellow serofluid culture medium is made；
S3, activation scenedesmus obliquus algae：Scenedesmus obliquus algae is activated with the selenium-rich yellow serofluid medium culture obtained in S2；
S4, algae are tentatively cultivated：Scenedesmus obliquus algae after being activated in selenium-rich yellow serofluid inoculation of medium S3, initial inoculation
Density is 0.95 × 106A/mL is irradiated using feux rouges fluorescent tube and is cultivated, and methyl jasmonate is added in intensity 1500-2000lux,
25 DEG C of static gas wave refrigerators, up to scenedesmus obliquus culture to exponential phase；
S5, algae Fiber differentiation：The cultivation temperature of the scenedesmus obliquus of exponential phase is increased to 32-37 DEG C, in blue light tube
Under irradiation, improves intensity of illumination and carry out Fiber differentiation, light dark period 21h to 7500-7700lux：It is trained in the culturing rack of 3h
It supports, adding in glucose and xylose to culture medium makes glucose and xylose concentration respectively reach 8-12g/L and 27.0-37.0g/L,
Opposite nitrogen lacks, and a concentration of the 0 of sodium nitrate, concussion shaking flask is primary daily, cultivates 15-20 days；
S6, chlorophyll and frond breaking-wall cell are destroyed：It takes out algae solution centrifugation in S5 and removes supernatant, collect frond precipitation, it is heavy in frond
The methanol solution that KOH solution and 18%-20% that mass fraction is 2%-4% are added in shallow lake destroys chlorophyll 8-10min, is collected by centrifugation
Precipitation, is dissolved in aqua sterilisa, then uses JN-6001 type cryogenic high pressure homogenizers（Guangdong Juneng Biology ＆ Technology Co., Ltd.）Into
Row cryogenic high pressure homogeneous broken wall treatment obtains the frond cell after broken wall；
S7, carotenoid extraction：The mass fraction that ice bath is crossed will be added in frond cell after broken wall in S6 as 87% ethyl alcohol
Solution carries out Extraction of carotenoid pigment, solid-liquid ratio 1:4.2, solid-liquid ratio unit is mg/mL, and extraction time is ice bath 40-
50min, repeats extraction time 2-3 times, until frond bleaches, filters and collect Extraction of carotenoid pigment liquid, carotenoid
Sucrose extraction is 93% or more；
It is prepared by S8, carotenoid dry product：By the Extraction of carotenoid pigment liquid in S7 by boiling off solvent, concentration, crystallization, vacuum are done
Carotenoid dry product is obtained after dry.
The astaxanthin-containing of scenedesmus obliquus algae is 0.09%, is cultivated by ultraviolet radioactive and the method for nitrosoguanidine Combined Stress
It forms, uses《Hubei agricultural science》In《Produce the cultivation of the scenedesmus obliquus algae of astaxanthin》In method cultivated to obtain
Scenedesmus obliquus newly tame algae (Scenedesmus obliquus New).
The selenium content of selenium-rich potato juice is 1.75-1.95mg/kg, and concentration selenium-rich spring selenium content is 20-24mg/L.
Feux rouges fluorescent tube is 22W, inside sets light adjusting knob, and feux rouges fluorescent tube is the left and right sides to light, both sides feux rouges fluorescent tube and algae
The distance between liquid is 12-14cm, in selenium-rich yellow serofluid culture medium methyl jasmonate concentration before exponential phase 3 days by
3.5mg/mL is increased to 5.25mg/mL.
Blue light tube is 20W, inside sets light adjusting knob, and blue light tube is top to light, the distance between top and algae solution
For 32-34cm, stress-inducing carotenoid.
The condition of cryogenic high pressure homogeneous broken wall is:9 DEG C, 70MPa, solid-liquid ratio 1:5.5, solid-liquid ratio unit is mg/mL,
Cycle 2-3 times.
The culturing rack uses the culture in the utility model patent " a kind of microdisk electrode case " that I has applied
Frame.
Embodiment 2
By taking ultrasonic wave auxiliary and ethyl alcohol extraction as an example
S1, yellow serofluid pretreatment：After yellow serofluid in bean product manufacturing process is collected, using 5-7 layers of filtered through gauze, after filtering
The pH values of yellow serofluid filtrate are adjusted to 7.5-8.0 using NaOH；
S2, selenium-rich yellow serofluid culture medium is produced：The richness that mass volume ratio is 8%-12% is added in the yellow serofluid filtrate made from S1
Selenium potato juice and the concentration selenium-rich spring that mass volume ratio is 25%-35%, yellow serofluid filtrate, selenium-rich potato juice and concentration are rich
The mass ratio of selenium spring is 7:2:1,121 DEG C, the sterilizing of 30min autoclavings are then used, selenium-rich yellow serofluid training is made
Support base；
S3, activation scenedesmus obliquus algae：Scenedesmus obliquus algae is activated with the selenium-rich yellow serofluid medium culture obtained in S2；
S4, algae are tentatively cultivated：Scenedesmus obliquus algae after being activated in selenium-rich yellow serofluid inoculation of medium S3, initial inoculation
Density is 0.95 × 106A/mL is irradiated using feux rouges fluorescent tube and is cultivated, and methyl jasmonate is added in intensity 1500-2000lux,
25 DEG C of static gas wave refrigerators, up to scenedesmus obliquus culture to exponential phase；
S5, algae Fiber differentiation：The cultivation temperature of the scenedesmus obliquus of exponential phase is increased to 32-37 DEG C, in blue light tube
Under irradiation, improves intensity of illumination and carry out Fiber differentiation, light dark period 21h to 7500-7700lux：It is trained in the culturing rack of 3h
It supports, adding in glucose and xylose to culture medium makes glucose and xylose concentration respectively reach 8-12g/L and 27.0-37.0g/L,
Concussion shaking flask is primary daily, cultivates 15-20 days；The utility model patent that the culturing rack uses me to apply is " a kind of
Culturing rack in microdisk electrode case ".
S6, chlorophyll and frond breaking-wall cell are destroyed：It takes out algae solution centrifugation in S5 and removes supernatant, frond precipitation is collected, in algae
The methanol solution that KOH solution and 18%-20% that mass fraction is 2%-4% are added in body precipitation destroys chlorophyll 8-10min, centrifugation
Precipitation is collected, is dissolved in aqua sterilisa, supersonic cell broken wall is carried out after dissolving；Ultrasonic wave assists the extraction of 38 DEG C of broken wall, when extraction
Between 45min, the sucrose extraction of carotenoid is relatively high；
S7, carotenoid extraction：The mass fraction that ice bath is crossed will be added in frond cell after broken wall in S6 as 75% ethyl alcohol
Solution, solid-liquid ratio 1:6, solid-liquid ratio unit is mg/mL, and extraction time is ice bath 40-50min, repeats extraction time 2-3
It is secondary, until frond bleaches, filters and collect Extraction of carotenoid pigment liquid；
It is prepared by S8, carotenoid dry product：By the Extraction of carotenoid pigment liquid in S7 by boiling off solvent, concentration, crystallization, vacuum are done
Carotenoid dry product is obtained after dry.
The astaxanthin-containing of scenedesmus obliquus algae is 0.09%, is cultivated by ultraviolet radioactive and the method for nitrosoguanidine Combined Stress
It forms, uses《Hubei agricultural science》In《Produce the cultivation of the scenedesmus obliquus algae of astaxanthin》In method cultivated to obtain
Scenedesmus obliquus newly tame algae (Scenedesmus obliquus New).
The selenium content of selenium-rich potato juice is 1.75-1.95mg/kg, and concentration selenium-rich spring selenium content is 20-24mg/L.
Feux rouges fluorescent tube is 22W, inside sets light adjusting knob, and feux rouges fluorescent tube is the left and right sides to light, both sides feux rouges fluorescent tube and algae
The distance between liquid is 12-14cm, in selenium-rich yellow serofluid culture medium methyl jasmonate concentration before exponential phase 3 days by
3.5mg/mL is increased to 5.25mg/mL.
Blue light tube is 20W, inside sets light adjusting knob, and blue light tube is top to light, the distance between top and algae solution
For 32-34cm, stress-inducing carotenoid.
The comparative experiments of two kinds of extracting methods is shown in Table 1：
Table 1
The results show that extraction time is all 45 min（That is class or so is especially suitable for the innovation undertaking of the groups such as student）'s
Under the conditions of, the method for the present invention preferred alcohol is extracted, and in the extracting method of selection, ethyl alcohol does not have toxic residue, and dosage is few, production
Product are safe.123.12 mg/ of natural carotenoid can be produced with method culture scenedesmus obliquus domestication novel species in embodiment 1
G dry weights, the carotenoid output extracted than method in embodiment 2 improve by about one time, and low temperature promotes recovery rate, and ethyl alcohol makes
It is improved with concentration, income increases.
This seminar was in national inventing patent " a kind of preparation method (Ling Shanfeng of selenium-rich astaxanthin chlorella powder in 2017
Deng patent disclosure (bulletin) number 107384907A）" enzyme process such as endogenous enzymes is used to combine high-pressure homogeneous extraction astaxanthin, it obtains
Excellent effect, so, this seminar be now conducted on further use the thick enzyme of shrimp head capsule endogenous enzymes or（With）Cellulose
The enzyme process assisted extraction scenedesmus obliquus natural carotenoid such as enzyme.
The above-mentioned only present invention preferably feasible embodiment, the non-therefore limitation scope of the present invention, according to above-described embodiment
Made various modifications are applied mechanically within this technical solution protection domain.
Claims (6)
1. a kind of method of culture scenedesmus obliquus algae production natural carotenoid, it is characterised in that include the following steps：
S1, yellow serofluid pretreatment：After yellow serofluid in bean product manufacturing process is collected, using 5-7 layers of filtered through gauze, after filtering
The pH values of yellow serofluid filtrate are adjusted to 7.5-8.0；
S2, selenium-rich yellow serofluid culture medium is produced：The richness that mass volume ratio is 8%-12% is added in the yellow serofluid filtrate made from S1
Selenium potato juice and the concentration selenium-rich spring that mass volume ratio is 25%-35%, yellow serofluid filtrate, selenium-rich potato juice and concentration are rich
The mass ratio of selenium spring is 7:2:1,121 DEG C, the sterilizing of 30min autoclavings are then used, selenium-rich yellow serofluid training is made
Support base；
S3, activation scenedesmus obliquus algae：Scenedesmus obliquus algae is activated with the selenium-rich yellow serofluid medium culture obtained in S2；
S4, algae are tentatively cultivated：Scenedesmus obliquus algae after being activated in selenium-rich yellow serofluid inoculation of medium S3, initial inoculation
Density is 0.95 × 106A/mL is irradiated using feux rouges fluorescent tube and is cultivated, and methyl jasmonate is added in intensity 1500-2000lux,
25 DEG C of static gas wave refrigerators, up to scenedesmus obliquus culture to exponential phase；
S5, algae Fiber differentiation：The cultivation temperature of the scenedesmus obliquus of exponential phase is increased to 32-37 DEG C, in blue light tube
Under irradiation, improves intensity of illumination and carry out Fiber differentiation, light dark period 21h to 7500-7700lux：It is trained in the culturing rack of 3h
It supports, adding in glucose and xylose to culture medium makes glucose and xylose concentration respectively reach 8-12g/L and 27.0-37.0g/L,
Concussion shaking flask is primary daily, cultivates 15-20 days；
S6, chlorophyll and frond breaking-wall cell are destroyed：It takes out algae solution centrifugation in S5 and removes supernatant, collect frond precipitation, it is heavy in frond
The methanol solution that KOH solution and 18%-20% that mass fraction is 2%-4% are added in shallow lake destroys chlorophyll 8-10min, is collected by centrifugation
Precipitation, is dissolved in aqua sterilisa, then carries out cryogenic high pressure homogeneous broken wall treatment or supersonic wave wall breaking handles to obtain the algae after broken wall
Body cell；
S7, carotenoid extraction：It is 75%-90%'s that the mass fraction that ice bath is crossed will be added in frond cell after broken wall in S6
Ethanol solution carries out Extraction of carotenoid pigment, solid-liquid ratio 1:4.2, solid-liquid ratio unit is mg/mL, and extraction time is ice bath 40-
50min, repeats extraction time 2-3 times, until frond bleaches, filters and collects Extraction of carotenoid pigment liquid；
It is prepared by S8, carotenoid dry product：By the Extraction of carotenoid pigment liquid in S7 by boiling off solvent, concentration, crystallization, vacuum are done
Carotenoid dry product is obtained after dry.
2. a kind of method of culture scenedesmus obliquus algae production natural carotenoid according to claim 1, feature exist
It is 0.09% in the astaxanthin-containing of scenedesmus obliquus algae, is cultivated by the method for ultraviolet radioactive and nitrosoguanidine Combined Stress.
3. a kind of method of culture scenedesmus obliquus algae production natural carotenoid according to claim 1, feature exist
It is 1.75-1.95mg/kg in the selenium content of selenium-rich potato juice, concentration selenium-rich spring selenium content is 20-24mg/L.
4. a kind of method of culture scenedesmus obliquus algae production natural carotenoid according to claim 1, feature exist
It is the left and right sides to light in feux rouges fluorescent tube, the distance between both sides feux rouges fluorescent tube and algae solution are 12-14cm, and selenium-rich yellow serofluid is trained
It supports methyl jasmonate concentration in base and 5.25mg/mL was increased to by 3.5mg/mL in 3 days before exponential phase.
5. a kind of method of culture scenedesmus obliquus algae production natural carotenoid according to claim 1, feature exist
It is top to light in blue light tube, the distance between top and algae solution are 32-34cm, stress-inducing carotenoid.
6. a kind of method of culture scenedesmus obliquus algae production natural carotenoid according to claim 1, feature exist
It is in the condition of cryogenic high pressure homogeneous broken wall:9 DEG C, 70MPa, solid-liquid ratio 1:5.5, solid-liquid ratio unit is mg/mL, recycles 2-3
It is secondary.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109456903A (en) * | 2018-12-29 | 2019-03-12 | 嘉必优生物技术（武汉）股份有限公司 | Selenium-rich richness carotenoid trispore Bruce mould, fermentative carotenoid product and fermentation process |
| CN110692848A (en) * | 2018-11-08 | 2020-01-17 | 荆楚理工学院 | Chicken feed containing selenium astaxanthin and DHA thereof |
| CN111802389A (en) * | 2020-08-07 | 2020-10-23 | 中国农业科学院茶叶研究所 | Exogenous induction composition, exogenous inducer and method for improving carotenoid in tea |
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| CN109456903B (en) * | 2018-12-29 | 2021-04-13 | 嘉必优生物技术（武汉）股份有限公司 | Selenium-rich carotenoid-rich Blakeslea trispora, carotenoid fermentation product and fermentation method |
| CN111802389A (en) * | 2020-08-07 | 2020-10-23 | 中国农业科学院茶叶研究所 | Exogenous induction composition, exogenous inducer and method for improving carotenoid in tea |
| CN111802389B (en) * | 2020-08-07 | 2021-12-28 | 中国农业科学院茶叶研究所 | Exogenous induction composition, exogenous inducer and method for improving carotenoid in tea |
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