CN1790002A - Reactor type instrument for quickly determining biochemical oxygen demand - Google Patents
Reactor type instrument for quickly determining biochemical oxygen demand Download PDFInfo
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- CN1790002A CN1790002A CN 200510086986 CN200510086986A CN1790002A CN 1790002 A CN1790002 A CN 1790002A CN 200510086986 CN200510086986 CN 200510086986 CN 200510086986 A CN200510086986 A CN 200510086986A CN 1790002 A CN1790002 A CN 1790002A
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Abstract
The invention discloses a BOD rapid detecting analyzer of water pollution monitor, which is characterized by the following: setting a cage in the biochemistry reaction measuring chamber; placing the solidified microbial particle in the cage; contacting the detected water sample in the biochemistry reaction measuring chamber directly through microscale aerated conduit; adapting metal hot container to cover the thermostatic device. The invention uses the aerated conduit instead of the magnetic blending device to accomplish rapid mass transformation and dissolved oxygen balance, which shortens the measuring time and changes the device periodically.
Description
Technical field
The present invention relates to a kind of Water Pollution Monitoring mesophytization oxygen demand (BOD) fast measuring analytical instrument, particularly a kind of structural design of reactor type instrument for quickly determining biochemical oxygen demand belongs to the environmental monitoring technology field.
Background technology
Adopt biology sensor to carry out the fast measuring of BOD, external typical products has the NisshinBOD-300 of Japan and the Biomonitor of Germany, the domestic MS-1BOD analyzer that Wuhan Virology Institute,Chinan academy of Sciences's development is arranged.Because MS-1BOD and BOD-300 are basic identical on principle and measuring method, only fluidised form is slightly different in the measuring chamber.
BOD-300 and Biomonitor instrument all are divided into four parts on function: sampling unit, water sample converting unit, measuring unit, control, data recording and processing unit, wherein measuring unit is the core structure of sensor.The remarkable different place of these two kinds of instruments just is the measurement state of microorganism in the measuring unit: microorganism is adopted biological membrane embedding mode in the BOD-300 instrument, be biological membrane and with tight contact of dissolved oxygen sensor probe, this structure also is moment sensor research and commercial typical structure.Microorganism is in the dispersion suspension state among the Biomonitor, thereby microorganism can directly contact with the test water sample.
Though the Biomonitor instrument of dispersion suspension state has solved the problem of biological membrane mode to a great extent, this measuring method also has the drawback of himself.The one, its employed microorganism need be measured the scene of need and be provided.Therefore it generally only is used for the mensuration of the BOD of sewage treatment plant concentration, but can't satisfy the natural water body monitoring away from sewage treatment plant.The 2nd, frequent replacing sensitive material microorganism will be organized the inconsistent problem that causes measuring the unified benchmark of shortage of microbial activity state because of each.
Microorganism is adopted the BOD-300 instrument of biological membrane embedding mode, though biological membrane is to be provided by the producer that produces instruments in the biological membrane embedding mode, the measurement desired microorganisms is guaranteed, but this instrument also exists following problem: one, what dissolved oxygen probe was measured in the instrument is not real dissolved oxygen content in the test water sample, but diffuse through biomembranous oxygen amount, make electric signal weaken 3-6 the order of magnitude.So this instrument not only needs good measurement environment, and the support of the split hair dissolved oxygen DO instrument of needs, this just makes the price of integral sensors rise, and reaches more than 10,000 dollars.They are two years old, the measurement range of this BOD determining instrument not only is subjected to the restriction of microorganism self metabolism, and controlled by the mass transfer of the perforated membrane of imbedded microbe, it is low to cause measuring linear range, generally only can arrive tens mg/litre, be lower than the BOD level of municipal effluent, measure again after can only diluting.
Deficiency and defective at above-mentioned instrument, patent CN1138141C discloses a kind of " biological sensor of oxygen amount needed biochemically ", this sensor adopts immobilization microorganism particles to combine with limited aeration by magnetic agitation with the mixed solution of water sample to be measured with buffer solution or buffer solution, can reach rapid mass transfer and dissolved oxygen DO equilibrium activity, effectively enlarge linear measurement range.But this device adopts the form of magnetic agitation to be difficult to control in the real reaction process, and magnetic stir bar is out of control easily, causes a whipping process need repeatedly start magnetic stirring apparatus, has increased minute greatly.In addition then, the immobilized microorganism in this device directly is placed in the biochemical reaction measuring chamber, causes the freeing port obstruction easily, is unfavorable for that also immobilized microorganism periodically takes out.In addition, this instrument is positioned over measuring chamber in the water bath with thermostatic control, causes the device overall volume bigger, and complex structure is unfavorable for the development of instrument miniaturization.
Summary of the invention
The purpose of this invention is to provide a kind of reactor type instrument for quickly determining biochemical oxygen demand, make it can not only effectively enlarge its linear measurement range, shorten Measuring Time, and simple in structure, easy to operate, and volume is little, and is in light weight, thereby is convenient to development and utilization.
Technical scheme of the present invention is as follows:
A kind of reactor type instrument for quickly determining biochemical oxygen demand, contain the biochemical reaction measuring chamber, be placed on the immobilization microorganism particles in the biochemical reaction measuring chamber, place the dissolved oxygen electrode of biochemical reaction measuring chamber, aerator and the thermostat that is arranged on biochemical reaction measuring chamber outside, it is characterized in that: be provided with a cylinder mould in the described biochemical reaction measuring chamber, immobilization microorganism particles is placed in the cylinder mould; Described aerator directly contacts with water sample to be measured in the biochemical reaction measuring chamber by the limited aeration pipe, and described thermostat adopts the metal fever inclusion body.
Metal fever inclusion body of the present invention is by metal shell, and the electric heating tube and the radiator valve that are arranged in the metal shell are formed.
The present invention compared with prior art, have the following advantages and the high-lighting effect: 1. the present invention has cancelled original magnetic stirring apparatus, replace stirring apparatus with suitable increase aeration rate, can reach rapid mass transfer and dissolved oxygen DO equilibrium activity, effectively enlarge linear measurement range (effective range of detection is 0-200mg/l), simplify testing process again, avoided the complicated pipelines design, shortened Measuring Time; 2. immobilized microorganism is put in the cylinder mould, both has been difficult for stopping up freeing port, also be convenient to immobilized microorganism and periodically change; 3. original constant temperature water bath apparatus is designed to the metal inclusion body, the overall volume of this instrument and weight are greatly reduced, have that volume is little, the characteristics of lightweight miniaturization, be convenient to exploitation and on-the-spot the use.
Description of drawings:
Fig. 1 is a reactor type instrument for quickly determining biochemical oxygen demand structure diagram provided by the invention.
Embodiment
Below in conjunction with accompanying drawing principle of work of the present invention, concrete structure and enforcement are further described.
Reactor type instrument for quickly determining biochemical oxygen demand provided by the invention, mainly comprise biochemical reaction measuring chamber 2, be placed on the immobilization microorganism particles 8 in the biochemical reaction measuring chamber, place the dissolved oxygen electrode 6 of biochemical reaction measuring chamber, with the limited aeration pipe 1 that water sample to be measured in the biochemical reaction measuring chamber directly contacts, be arranged on cylinder mould 7 in the biochemical reaction measuring chamber and the metal fever inclusion body 3 that is arranged on biochemical reaction measuring chamber outside.The metal fever inclusion body is by metal shell, is arranged on electric heating tube 4, temperature sensor 9 and the thermostatically-controlled equipment 5 that links to each other with temperature sensor with electric heating tube in the housing.
During measurement, immobilization microorganism particles 8 is placed in the cylinder mould 7, and immobilization microorganism particles mixes in biochemical reaction measuring chamber 1 with testing sample, stirs and aeration with limited aeration pipe 1, make that wherein dissolved oxygen DO is saturated, continue limited aeration and reduce to a new steady state (SS) until dissolved oxygen DO.Difference between two stable states of dissolved oxygen DO is promptly represented the BOD value of the solution of surveying.The present invention can make the microorganism in the biochemical reaction measuring chamber be in suspended state by suitably improving aeration rate (400-600ml/min), has cancelled original magnetic stirring apparatus like this, has simplified the structure of measuring chamber.When measuring beginning, microorganism is in the endogenous respiration state, and the dissolved oxygen concentration of measuring chamber empty solution keeps steady state (SS).After injecting the test water sample, microorganism transfers external source to and breathes, and the dissolved oxygen concentration in the solution continues to descend, until reaching a new steady state (SS).The difference between these two dissolved oxygen stable states and the BOD concentration of water sample have metastable linear dependence relation in the scope of 0～200mg/l.
Embodiment
Carry out the mensuration of standard BOD sample with instrument provided by the present invention.
Disposing BOD concentration respectively is 5mg/l, 20mg/l, and 50mg/l, 100mg/l, the standard GGA solution of 200mg/l is made 15min and is detected.Test condition is: 30 ± 1 ℃ of water-baths, and the deionized water dilution, aeration rate is 400-600ml/min.It is the shortest that the BOD value reaches equilibration time for the GGA titer of 5mg/l detects, 5min consuming time, and BOD value is the longest for the GGA titer detection of 200mg/l reaches the balance required time, is 13min.
Testing result shows that the sensing range of this instrument is 0-200mg/l, and measuring the linear related coefficient that is reached is 0.996, satisfies the requirement of actual waste water on-line measurement.
The linearly dependent coefficient of typical curve is 0.9908, and standard equation is Δ DO=0.0082 * BOD.
Claims (2)
1. reactor type instrument for quickly determining biochemical oxygen demand, contain biochemical reaction measuring chamber (2), be placed on the immobilization microorganism particles (8) in the biochemical reaction measuring chamber, place the dissolved oxygen electrode (6) of biochemical reaction measuring chamber, aerator and the thermostat that is arranged on biochemical reaction measuring chamber outside, it is characterized in that: be provided with a cylinder mould (7) in the described biochemical reaction measuring chamber, immobilization microorganism particles is placed in the cylinder mould; Described aerator directly contacts with water sample to be measured in the biochemical reaction measuring chamber by limited aeration pipe (1), and described thermostat adopts metal fever inclusion body (3).
2. according to the described reactor type instrument for quickly determining biochemical oxygen demand of claim 1, it is characterized in that: described metal fever inclusion body is by metal shell, and the electric heating tube (4), temperature sensor (9) and the radiator valve (5) that are arranged in the metal shell are formed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510086986 CN1790002A (en) | 2005-11-25 | 2005-11-25 | Reactor type instrument for quickly determining biochemical oxygen demand |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510086986 CN1790002A (en) | 2005-11-25 | 2005-11-25 | Reactor type instrument for quickly determining biochemical oxygen demand |
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| CN1790002A true CN1790002A (en) | 2006-06-21 |
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| CN 200510086986 Pending CN1790002A (en) | 2005-11-25 | 2005-11-25 | Reactor type instrument for quickly determining biochemical oxygen demand |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101358959A (en) * | 2008-05-07 | 2009-02-04 | 江苏同和涂装机械有限公司 | Lifting mechanism of regular microorganism particle in biosensor BOD rapid measuring instrument |
| WO2011022921A1 (en) * | 2009-08-31 | 2011-03-03 | 清华大学 | Reactor-type rapid measuring instrument for biochemical oxygen demand (bod) and measurement method thereof |
| CN101000341B (en) * | 2006-12-29 | 2011-09-28 | 中国科学院长春应用化学研究所 | Sensor type on-line monitor of biochemical oxygen demand |
| CN101413915B (en) * | 2008-12-01 | 2012-05-16 | 中国科学院长春应用化学研究所 | Method for rapidly detecting biochemical oxygen demand by using on site culture organism |
| CN104267166A (en) * | 2014-10-13 | 2015-01-07 | 吉林市光大分析技术有限责任公司 | Rapid biological oxygen consumption detection device |
| CN109061208A (en) * | 2018-08-02 | 2018-12-21 | 上海宝中盈仪器仪表有限公司 | Full-automatic biochemical oxygen demand detector |
| CN111982746A (en) * | 2020-07-16 | 2020-11-24 | 北京城市排水集团有限责任公司 | Aeration performance evaluation method of aeration device |
-
2005
- 2005-11-25 CN CN 200510086986 patent/CN1790002A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101000341B (en) * | 2006-12-29 | 2011-09-28 | 中国科学院长春应用化学研究所 | Sensor type on-line monitor of biochemical oxygen demand |
| CN101358959A (en) * | 2008-05-07 | 2009-02-04 | 江苏同和涂装机械有限公司 | Lifting mechanism of regular microorganism particle in biosensor BOD rapid measuring instrument |
| CN101413915B (en) * | 2008-12-01 | 2012-05-16 | 中国科学院长春应用化学研究所 | Method for rapidly detecting biochemical oxygen demand by using on site culture organism |
| WO2011022921A1 (en) * | 2009-08-31 | 2011-03-03 | 清华大学 | Reactor-type rapid measuring instrument for biochemical oxygen demand (bod) and measurement method thereof |
| CN104267166A (en) * | 2014-10-13 | 2015-01-07 | 吉林市光大分析技术有限责任公司 | Rapid biological oxygen consumption detection device |
| CN104267166B (en) * | 2014-10-13 | 2016-05-11 | 吉林市光大分析技术有限责任公司 | BOD device for fast detecting |
| CN109061208A (en) * | 2018-08-02 | 2018-12-21 | 上海宝中盈仪器仪表有限公司 | Full-automatic biochemical oxygen demand detector |
| CN111982746A (en) * | 2020-07-16 | 2020-11-24 | 北京城市排水集团有限责任公司 | Aeration performance evaluation method of aeration device |
| CN111982746B (en) * | 2020-07-16 | 2021-06-11 | 北京城市排水集团有限责任公司 | Aeration performance evaluation method of aeration device |
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