CN102262123A - Quick biochemical oxygen demand (BOD) measuring instrument for reactor type biosensor - Google Patents
Quick biochemical oxygen demand (BOD) measuring instrument for reactor type biosensor Download PDFInfo
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- CN102262123A CN102262123A CN2010101868598A CN201010186859A CN102262123A CN 102262123 A CN102262123 A CN 102262123A CN 2010101868598 A CN2010101868598 A CN 2010101868598A CN 201010186859 A CN201010186859 A CN 201010186859A CN 102262123 A CN102262123 A CN 102262123A
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Abstract
The invention relates to a quick biochemical oxygen demand (BOD) measuring instrument for a reactor type biosensor, and belongs to a water pollution monitoring instrument. In the quick BOD measuring instrument, an integrated control system and a liquid inlet/outlet control system are combined, and a temperature sensor, a liquid level probe, an electrode probe, a heating sheet and a magnetic force generator are subjected to integrated control by the computer integrated control system; the liquid inlet/outlet control system is controlled by the computer integrated control system; and each process is introduced into a program to realize automated management control. The temperature in a reactor is controlled accurately, so that microbes always keep the activity on the same horizontal line for inflow, outflow and aeration. Dissolved oxygen consumed at a reaction stage can be controlled to the same quantity, and the dissolved oxygen in water reaches balance and dynamic balance by stirring and the aeration. Granules react with organisms to consume the dissolved oxygen in the water, and a corresponding BOD value is measured further by measuring a difference value formed before and after the dissolved oxygen is dissolved. The measuring instrument is simply, conveniently, quickly and accurately operated, high in automation degree, convenient to carry, wide in application range and high in efficiency, and can display figures of the measured BOD automatically.
Description
Technical field
The present invention relates to a kind of Water Pollution Monitoring instrument, particularly a kind of reactor type biology sensor BOD fast measuring instrument.
Background technology
Along with society, science and technology fast development, problems such as chemical industry, environmental pollution have caused the attention of countries in the world.The discharge of wastewater of chemical industry directly has influence on water pollution, is related to the healthy of the mankind, vegeto-animal existence.In water body, exist miscellaneous organic contaminant, be difficult to determine respectively the quantitative value of various components, thereby biochemical oxygen demand (BiochemicalOxygen Demand, BOD), in the water quality assessment process, obtained using widely as a kind of composite target that characterizes the organic contamination degree.BOD represents the dissolved oxygen content that organism is consumed in the water body under microbiological oxidation, unit is mg/L, represents the pollution level of biodegradable part organic contaminant in the water body.At present: 5 days 20 ℃ of cultivations of external and domestic main employing are measured the BOD value in the water samples, are designated as BOD
5Yet, there are many weak points in this method, as measure big, the unsuitable field monitoring of cycle length, complicated operation, poor reproducibility, interference etc., and: existing metering system all is to adopt biological membrane as sensitive element, make to have implacable contradiction between biomass and the biological membrane thickness, consider, need more biomass from the sensitivity that detects; And consider that from the angle of mass transfer wish that then biological membrane is thinner, therefore, traditional BOD sensor measurement instability, poor reproducibility fail to be used widely.At home in the same industry: R﹠D institution and science and technology in enterprise personnel are at the structure of constantly studying, explore, improve reactor type biology sensor BOD fast measuring instrument, press for a kind of simple to operate, change the degree height accurately, fast and automatically, new method that usable range is wide is measured BOD, though obtain some progress technically, but in practice, still exist the technical barrier that does not overcome as yet, wish to work out the reactor type biology sensor BOD fast measuring instrument of high stability and high accuracy.
Summary of the invention
The objective of the invention is to overcome above deficiency, a kind of reactor type biology sensor BOD fast measuring instrument is provided, overcome the problem that exists in the biological pattern measuring method, adopt integrated control system to combine, utilize the computing machine integrated control system that temperature sensor, liquid-level probe, electrode probe, heating plate, magnetic force generator are carried out integrated control with the liquid in-out control system.The liquid in-out control system is made up of run-down pipe, drain pipe, aeration tube, feed tube, control by the computing machine integrated control system simultaneously, program is enrolled in each road technological process, unified management, accurately the temperature in the controlling reactor makes activation of microorganism remain at water inlet and water outlet and aeration on the same horizontal line.The dissolved oxygen DO that the stage of reaction consumed just can be controlled at quantitatively same, stirs with aeration to make the dissolved oxygen DO in the water reach balance and mobile equilibrium.Particle and biological respinse, dissolved oxygen DO in the consume water, determine the dissolving before and after difference, at the difference inverse iteration in typical curve, corresponding BOD value can be measured, and the biological respinse difference that the organic contaminant in microbe granular and the water sample takes place can be effectively controlled.This analyzer is simple to operation, quick, accurate, automated procedures are high, can show the numeral of measuring BOD automatically, and is easy to carry, applied widely, the efficient height.
The technical solution adopted for the present invention to solve the technical problems is: contain integrated control system, the liquid in-out control system, temperature sensor, liquid-level probe, electrode probe, heating plate, run-down pipe, drain pipe, the magnetic force generator, magnetic bar, the microorganism cylinder mould, glass reactor, plexiglass tent, screw rod supports, lower bottom base, slide is supported in lifting, feed tube, the lifter slide block, aeration tube, the upper support seat, the cylinder mould cross bar, lower bottom base is a square shape, four jiaos are provided with screw rod and support with the upper support seat and be connected to form a framework on lower bottom base, in framework, be provided with plexiglass tent, in plexiglass tent, be provided with glass reactor, the upper support seat is a square, the centre is provided with circular hole and glass reactor upper end bore matches, the right-hand upper end of glass reactor is provided with aeration tube, the right-hand lower end of glass reactor is provided with feed tube, the glass reactor lower end is connected with the magnetic force generator, the lower end of magnetic force generator is connected with lower bottom base, above the magnetic force generator, be provided with heating plate, a magnetic bar is arranged above heating plate, be provided with lifting on the right side of lower bottom base and support slide, on lifting support slide, be provided with the lifter slide block, the top of lifter slide block is connected with an end of cylinder mould cross bar, the cylinder mould cross bar other end hangs the microorganism cylinder mould, the microorganism cylinder mould is arranged in the glass reactor, temperature sensor, liquid-level probe, electrode probe is arranged in the glass reactor, temperature sensor, electrode probe, liquid-level probe, heating plate, the electronic circuit of magnetic force generator is connected with integrated control system, integrated control system adopts integrated package control, on integrated control system, be provided with display screen and digital open-close on-off, the liquid in-out control system is provided with run-down pipe and drain pipe, the other end of run-down pipe is connected with the upper end of glass reactor, the other end of drain pipe is connected with the lower end of glass reactor, has constituted a complete reactor type biology sensor BOD fast measuring instrument.
The know-why that this reactor type biology sensor BOD fast measuring instrument adopts is: adopt integrated control system to combine with the liquid in-out control system, utilize the computing machine integrated control system that temperature sensor, liquid-level probe, electrode probe, heating plate, magnetic force generator are carried out integrated control.The liquid in-out control system is made up of run-down pipe, drain pipe, aeration tube, feed tube, control by the computing machine integrated control system simultaneously, program is enrolled in each road technological process, unified management, accurately the temperature in the controlling reactor makes activation of microorganism remain at water inlet and water outlet and aeration on the same horizontal line.The dissolved oxygen DO that the stage of reaction consumed just can be controlled at quantitatively same, stirs with aeration to make the dissolved oxygen DO in the water reach balance and mobile equilibrium.Particle and biological respinse, dissolved oxygen DO in the consume water, determine the dissolving before and after difference, at the difference inverse iteration in typical curve, corresponding BOD value can be measured, and the biological respinse difference that the organic contaminant in microbe granular and the water sample takes place can be effectively controlled.When starting analyzer, the entry into service of liquid in-out control system, the feed tube that is positioned at the glass reactor bottom righthand side passes plexiglass tent, input sewage to be measured is to the run-down pipe upper end in glass reactor, sewage to be measured floods probe, liquid-level probe, electrode probe and the microorganism cylinder mould of temperature sensor, the sewage to be measured that has more flow in the liquid in-out control system by run-down pipe, guarantees to detect quality.Immobilization microorganism particles in the microorganism cylinder mould mixes in glass reactor with the sewage to be measured of input, the magnetic force generator begins reaction, the heating plate of top begins heating, heating plate is the part of temperature control system, microbial reaction need carry out under the condition of constant temperature, by the heating of heating plate, add heat control temperature conditions to water sample and reach predetermined measurement temperature, make solution keep the effect of constant temperature.The magnetic bar of heating plate top begins rotation by the magneticaction of magnetic force generator after the energising, make microorganism mix, stir with sewage to be measured, makes mixeding liquid temperature and dissolved oxygen content even.The aeration tube that is positioned at the glass reactor upper right side carries out aeration to mixed liquor, airborne oxygen is forced to shift to mixed liquor, make mixed liquor obtain enough dissolved oxygen DOs, and prevent that the mixed liquor in the glass reactor from producing precipitation, the interior organism of reinforced glass reactor contacts with microbe granular and dissolved oxygen DO, guarantee that microorganism is having under the condition of sufficient dissolved oxygen DO, to organic oxygenolysis in the sewage to be measured.Electrode probe can be measured the dissolved oxygen content in the mixed solution, dissolved oxygen content what directly reaction be what of the voltage signal of electrode output, the voltage signal of exporting by the processing electrode, the dissolved oxygen DO in can volumetric solution.Liquid-level probe is surveyed solution level in the glass reactor, the solution amount in the control glass reactor, and when solution flooded probe, output signal control liquid in-out control system was closed work.In glass reactor, utilize biosensor principle, with immobilization microorganism particles as biological identification element, organism in the sewage to be measured is with after microorganism contacts, decomposed by microorganism, the variation that the dissolved oxygen concentration of linear relationship is arranged with BOD be can monitor out, the stability of analyzer, the reappearance and the accuracy of measurement result improved.Be arranged in starting point and terminal point that microorganism cylinder mould on right-hand lifter, cylinder mould cross bar, lifter slide block, lifting support the organic contaminant generation biochemical reaction of control immobilization microorganism particles that slide can be strict and water sample, guarantee the stability that analyzer is measured, after measurement is finished, the lifter slide block that is positioned on the lifting support slide will up slide, thereby drive the cylinder mould cross bar and do upward movement, make the microorganism cylinder mould leave sewage to be measured, simultaneously, sewage to be measured is also discharged by drain pipe input liquid in-out control system.The concentration of wastewater and the voltage difference that show different time sections on the display screen of integrated control system obtain calculating the data of BOD.Be provided with bolt between the upper support seat of analyzer and the lower bottom base and support, make whole device firm, stable.
Beneficial effect of the present invention is: adopt integrated control system to combine with the liquid in-out control system, utilize the computing machine integrated control system that temperature sensor, liquid-level probe, electrode probe, heating plate, magnetic force generator are carried out integrated control.The liquid in-out control system is made up of run-down pipe, drain pipe, aeration tube, feed tube, control by the computing machine integrated control system simultaneously, program is enrolled in each road technological process, realization automatic management control, accurately the temperature in the controlling reactor makes activation of microorganism remain at water inlet and water outlet and aeration on the same horizontal line.The dissolved oxygen DO that the stage of reaction consumed just can be controlled at quantitatively same, stirs with aeration to make the dissolved oxygen DO in the water reach balance and mobile equilibrium.Particle and biological respinse, dissolved oxygen DO in the consume water, determine the dissolving before and after difference, at the difference inverse iteration in typical curve, corresponding BOD value can be measured, and the biological respinse difference that the organic contaminant in microbe granular and the water sample takes place can be effectively controlled.This analyzer is simple to operation, quick, accurate, automated procedures are high, can show the numeral of measuring BOD automatically, and is easy to carry, applied widely, the efficient height.
Description of drawings
Be in conjunction with the accompanying drawings and embodiments the present invention to be further described below:
It among the figure reactor type biology sensor BOD fast measuring instrument structural representation
In the drawings: 1. integrated control system, 2. the liquid in-out control system, 3. temperature sensor, 4. liquid-level probe, 5. electrode probe, 6. heating plate, 7. run-down pipe, 8. drain pipe, 9. the magnetic force generator, 10. magnetic bar, 11. microorganism cylinder moulds, 12. glass reactors, 13. plexiglass tents, the support of 14. screw rods, 15. lower bottom bases, 16. liftings support slide, 17. feed tubes, 18. lifter slide blocks, 19. aeration tubes, 20. upper support seats, 21. cylinder mould cross bars
Embodiment
In the drawings: lower bottom base 15 is square shapes, four jiaos are provided with screw rod and support 14 and be connected to form a framework with upper support seat 20 on lower bottom base 15, in framework, be provided with plexiglass tent 13, in plexiglass tent 13, be provided with glass reactor 12, upper support seat 20 is squares, the centre is provided with circular hole and glass reactor 12 upper end bores match, the right-hand upper end of glass reactor 12 is provided with aeration tube 19, glass reactor 12 right-hand lower ends are provided with feed tube 17, glass reactor 12 lower ends are connected with magnetic force generator 9, the lower end of magnetic force generator 9 is connected with lower bottom base 15, above magnetic force generator 9, be provided with heating plate 6, a magnetic bar 10 is arranged above heating plate 6, be provided with lifting on the right side of lower bottom base 15 and support slide 16, on lifting support slide 16, be provided with lifter slide block 18, the top of lifter slide block 18 is connected with an end of cylinder mould cross bar 21, cylinder mould cross bar 21 other ends hang microorganism cylinder mould 11, microorganism cylinder mould 11 is arranged in the glass reactor 12, temperature sensor 3, liquid-level probe 4, electrode probe 5 is arranged in the glass reactor 12, temperature sensor 3, electrode probe 5, liquid-level probe 4, heating plate 6, the electronic circuit of magnetic force generator 9 is connected with integrated control system 1, integrated control system 1 adopts integrated package control, on integrated control system 1, be provided with display screen and digital open-close on-off, liquid in-out control system 2 is provided with run-down pipe 7 and drain pipe 8, the other end of run-down pipe 7 is connected with the upper end of glass reactor 12, the other end of drain pipe 8 is connected with the lower end of glass reactor 12, has constituted a complete reactor type biology sensor BOD fast measuring instrument.
Adopt integrated control system 1 to combine, utilize computing machine integrated control system 1 that temperature sensor 3, liquid-level probe 4, electrode probe 5, heating plate 6, magnetic force generator 9 are carried out integrated control with liquid in-out control system 2.Liquid in-out control system 2 is made up of run-down pipe 7, drain pipe 8, aeration tube 19, feed tube 17, simultaneously by 1 control of computing machine integrated control system, program is enrolled in each road technological process, unified management, accurately the temperature in the controlling reactor makes activation of microorganism remain at water inlet and water outlet and aeration on the same horizontal line.The dissolved oxygen DO that the stage of reaction consumed just can be controlled at quantitatively same, stirs with aeration to make the dissolved oxygen DO in the water reach balance and mobile equilibrium.Particle and biological respinse, dissolved oxygen DO in the consume water, determine the dissolving before and after difference, at the difference inverse iteration in typical curve, corresponding BOD value can be measured, and the biological respinse difference that the organic contaminant in microbe granular and the water sample takes place can be effectively controlled.When starting analyzer, 2 entrys into service of liquid in-out control system, the feed tube 17 that is positioned at glass reactor 12 bottom righthand sides passes plexiglass tent 13, input sewage to be measured is to run-down pipe 7 upper ends in glass reactor 12, sewage to be measured floods probe, liquid-level probe 4, electrode probe 5 and the microorganism cylinder mould 11 of temperature sensor 3, the sewage to be measured that has more flow in the liquid in-out control system 2 by run-down pipe 7, guarantees to detect quality.Immobilization microorganism particles in the microorganism cylinder mould 11 mixes in glass reactor 12 with the sewage to be measured of input, magnetic force generator 9 begins reaction, the heating plate 6 of top begins heating, heating plate 6 is parts of temperature control system, microbial reaction need carry out under the condition of constant temperature, by the heating of heating plate 6, add heat control temperature conditions to water sample and reach predetermined measurement temperature, make solution keep the effect of constant temperature.The magnetic bar 10 of heating plate 6 tops begins rotation by the magneticaction of magnetic force generator 9 after the energising, make microorganism mix, stir with sewage to be measured, makes mixeding liquid temperature and dissolved oxygen content even.The 19 pairs of mixed liquors of aeration tube that are positioned at glass reactor 12 upper right sides carry out aeration, airborne oxygen is forced to shift to mixed liquor, make mixed liquor obtain enough dissolved oxygen DOs, and prevent that the mixed liquor in the glass reactor 12 from producing precipitation, reinforced glass reactor 12 interior organism contact with microbe granular and dissolved oxygen DO, guarantee that microorganism is having under the condition of sufficient dissolved oxygen DO, to organic oxygenolysis in the sewage to be measured.Electrode probe 5 can be measured the dissolved oxygen content in the mixed solution, dissolved oxygen content what directly reaction be what of the voltage signal of electrode output, the voltage signal of exporting by the processing electrode, the dissolved oxygen DO in can volumetric solution.Liquid-level probe 4 is surveyed solution level in the glass reactor 12, the solution amount in the control glass reactor 12, and when solution flooded probe, output signal control liquid in-out control system 2 was closed work.In glass reactor 12, utilize biosensor principle, with immobilization microorganism particles as biological identification element, organism in the sewage to be measured is with after microorganism contacts, decomposed by microorganism, the variation that the dissolved oxygen concentration of linear relationship is arranged with BOD be can monitor out, the stability of analyzer, the reappearance and the accuracy of measurement result improved.Be arranged in starting point and terminal point that microorganism cylinder mould 11 on right-hand lifter, cylinder mould cross bar 21, lifter slide block 18, lifting support the organic contaminant generation biochemical reaction of control immobilization microorganism particles that slide 16 can be strict and water sample, guarantee the stability that analyzer is measured, after measurement is finished, the lifter slide block 18 that is positioned on the lifting support slide 16 will up slide, thereby drive cylinder mould cross bar 21 and do upward movement, make microorganism cylinder mould 11 leave sewage to be measured, simultaneously, sewage to be measured is also discharged by drain pipe 8 input liquid in-out control system 2.The concentration of wastewater and the voltage difference that show different time sections on the display screen of integrated control system 1 obtain calculating the data of BOD.Be provided with bolt between the upper support seat 20 of analyzer and the lower bottom base 15 and support, make whole device firm, stable.This reactor type biology sensor method BOD fast measuring instrument utilizes the brand-new biological identification element of immobilization microorganism particles as BOD fast measuring instrument, broken through and adopted the defective of biological membrane as biological identification element, invented reactor type biology sensor method BOD fast measuring instrument, compare with traditional biological membrane type reactor, dissolved oxygen DO, the diffusional resistance of organic molecule significantly reduces, and full contact with microorganism, improved the stability and the accuracy of measurement result, this method has successfully overcome the poor reproducibility that traditional biomembrance process exists, shortcomings such as sensitivity is low.
Claims (1)
1. reactor type biology sensor BOD fast measuring instrument, contain integrated control system, the liquid in-out control system, temperature sensor, liquid-level probe, electrode probe, heating plate, run-down pipe, drain pipe, the magnetic force generator, magnetic bar, the microorganism cylinder mould, glass reactor, plexiglass tent, screw rod supports, lower bottom base, slide is supported in lifting, feed tube, the lifter slide block, aeration tube, the upper support seat, the cylinder mould cross bar, it is characterized in that: lower bottom base is a square shape, four jiaos are provided with screw rod and support with the upper support seat and be connected to form a framework on lower bottom base, in framework, be provided with plexiglass tent, in plexiglass tent, be provided with glass reactor, the upper support seat is a square, the centre is provided with circular hole and glass reactor upper end bore matches, the right-hand upper end of glass reactor is provided with aeration tube, the right-hand lower end of glass reactor is provided with feed tube, the glass reactor lower end is connected with the magnetic force generator, the lower end of magnetic force generator is connected with lower bottom base, above the magnetic force generator, be provided with heating plate, a magnetic bar is arranged above heating plate, be provided with lifting on the right side of lower bottom base and support slide, on lifting support slide, be provided with the lifter slide block, the top of lifter slide block is connected with an end of cylinder mould cross bar, the cylinder mould cross bar other end hangs the microorganism cylinder mould, the microorganism cylinder mould is arranged in the glass reactor, temperature sensor, liquid-level probe, electrode probe is arranged in the glass reactor, temperature sensor, electrode probe, liquid-level probe, heating plate, the electronic circuit of magnetic force generator is connected with integrated control system, integrated control system adopts integrated package control, on integrated control system, be provided with display screen and digital open-close on-off, the liquid in-out control system is provided with run-down pipe and drain pipe, the other end of run-down pipe is connected with the upper end of glass reactor, the other end of drain pipe is connected with the lower end of glass reactor, has constituted a complete reactor type biology sensor BOD fast measuring instrument.
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Cited By (5)
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CN102297888A (en) * | 2010-06-25 | 2011-12-28 | 江苏同和涂装机械有限公司 | Magnetic stirring type aeration and constant-temperature reaction device |
CN103645224A (en) * | 2013-12-02 | 2014-03-19 | 中山欧麦克仪器设备有限公司 | On-line BOD (biochemical oxygen demand) measurer |
CN104267082A (en) * | 2014-10-14 | 2015-01-07 | 四川农业大学 | Method for rapidly measuring BOD (biochemical oxygen demand) by collagenous fiber immobilized escherichia coli serving as biosensor recognition element |
CN104267166A (en) * | 2014-10-13 | 2015-01-07 | 吉林市光大分析技术有限责任公司 | Rapid biological oxygen consumption detection device |
CN104289163A (en) * | 2014-09-29 | 2015-01-21 | 四川氟迪新能源有限公司 | Adjustable damping reactor base |
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102297888A (en) * | 2010-06-25 | 2011-12-28 | 江苏同和涂装机械有限公司 | Magnetic stirring type aeration and constant-temperature reaction device |
CN103645224A (en) * | 2013-12-02 | 2014-03-19 | 中山欧麦克仪器设备有限公司 | On-line BOD (biochemical oxygen demand) measurer |
CN104289163A (en) * | 2014-09-29 | 2015-01-21 | 四川氟迪新能源有限公司 | Adjustable damping reactor base |
CN104267166A (en) * | 2014-10-13 | 2015-01-07 | 吉林市光大分析技术有限责任公司 | Rapid biological oxygen consumption detection device |
CN104267166B (en) * | 2014-10-13 | 2016-05-11 | 吉林市光大分析技术有限责任公司 | BOD device for fast detecting |
CN104267082A (en) * | 2014-10-14 | 2015-01-07 | 四川农业大学 | Method for rapidly measuring BOD (biochemical oxygen demand) by collagenous fiber immobilized escherichia coli serving as biosensor recognition element |
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Application publication date: 20111130 |