CN1786692A - Process for determining content of potassium ion by enzyme method and kit for diagnosing potassium ion thereof - Google Patents
Process for determining content of potassium ion by enzyme method and kit for diagnosing potassium ion thereof Download PDFInfo
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- CN1786692A CN1786692A CN 200410066183 CN200410066183A CN1786692A CN 1786692 A CN1786692 A CN 1786692A CN 200410066183 CN200410066183 CN 200410066183 CN 200410066183 A CN200410066183 A CN 200410066183A CN 1786692 A CN1786692 A CN 1786692A
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Abstract
The invention relates to a method to measure potassium ion content by enzyme method and potassium ion diagnosing kit. The adenosine diphosphate and phosphoric acid enol pyruvic acid take reaction to form into pyruvic acid by utilizing the characteristic of potassium ion activate pyruvate kinase, and pyruvic acid would take reaction with lactate dehydrogenase to form into lactic acid, and at the same time the reduced coenzyme would oxygenated into oxygenated type coenzyme, by measuring the rate of decent of dominant wavelength at 340nm absorbance to quantitative response the content of potassium ion in sample. The invention has high specificity, and has no impact from the sodion in the sample.
Description
Technical field
The present invention relates to the method for potassium content in the samples such as enzymatic assays serum, blood plasma, and use the formulated potassium ion diagnostic kit of this method, belong to medical test determination techniques field.
Background technology
Potassium content in the serum (being called for short " blood potassium ") has very important meaning clinically, and blood in human body in potassium has rational reference range under the normal condition.When being lower than under the reference value, blood potassium prescribes a time limit, show hypopotassaemia, its reason has renal tubular disease, high aldosterone disease, hyperinsulinism, metabolic alkalosis, or uses the insulin for treating diabetes ketosis, uses in some lysis of diuretic therapy potassium ion to transfer to cell interior etc.; Blood potassium is higher than on the reference value in limited time, may be because renal glomerular disease, renal function are weak, the poisoning of diabetes ketosis, hypoadrenocorticism etc. cause; When blood potassium meets or exceeds 7.5mmol/l, arrhythmia cordis will appear in patient, should consider to determine suitable therapeutic scheme; Blood potassium surpasses 10mmol/l, ventricular fibrillation, cardiac arrest can take place and death.High potassium makes cardiac arrest diastole.
The common method of measuring potassium content in the prior art has: isotope dilution mass spectrometry, neutron activation method, flare photometer, chemical assay, ion selective electrode method, atom spectrophotometric method, enzyme kinetics method etc.Wherein, decisive method is isotope dilution mass spectrometry and neutron activation method, secondly is flare photometer, ion selective electrode method.
Flare photometer---nineteen fifty is brought into use and uses till today always, can detect the Na in serum, urine, cerebrospinal fluid and the ascites pleural fluid
+And K
+, be a kind of emission spectrometry method, assay method is divided into internal reference method and two kinds of outer standard laws.Outer standard law operate miss is bigger, does not generally adopt.The main now internal standard method of using is promptly added the internal standard element lithium or the caesium of same concentrations and is measured in sample and titer, during operation, the solution that will contain lithium is measured K simultaneously as dilution
+, Na
+And Li
+Concentration, with the Na of sample and titer
+/ Li
+With K
+/ Li
+Ratio calculates Na
+, K
+Concentration.Because serum diluting multiple is big, the influence of serum proteins viscosity almost can be ignored.
Ion selective electrode method---being called for short the ISE method, is to adopt sensitive specific electrode special, the K in body fluid such as the cleer and peaceful urine of the enterprising promoting circulation of blood of instrumentation
+, Na
+Mensuration, because of the sample consumption is few, quick and precisely, the trend that replaces other method is arranged almost.Its measuring principle is, combines with ion-selective electrode and contrast electrode, is immersed in the sample solution to be measured and detects.Present existing type of electrodes has: the glass-membrane electrode, inductive material is a glass film, and PH electrode, K are arranged
+Electrode and Na
+Electrode; immobilon-p electrode by the extrusion forming of slightly solubility metallics, as sense film, has Cl with solid film or odd-numbered day film
-Electrode and F
-Electrode; liquid film electrode as sense film, has Ca with epoxy resin or interior dress polyvinyl chloride
2+Electrode; The K that makes with the valinomycins film
+Electrode.Above-mentioned electrode all has certain serviceable life because electrode use after a period of time will automatic aging, the term of validity is different in size.Ion selective electrode method application at present is comparatively general, but electrode cost costliness.
In addition, chemical assay mainly utilizes multiple ring crown compound such as cave crown ether or spherical crown ether, measures as ionophore.Owing in the macrocyclic structure hole is arranged, intramolecular oxygen atom has not, and share electron pair can combine with metallic ion, according to the hole size, alternative metallic ion in conjunction with different-diameter, thereby reach the purpose (measure blood potassium, generally adopt common crown ether anionic dye to carry out colorimetric assay) of measuring ion concentration; The atom spectrophotometric method also can be used for detecting K in the serum
+, Na
+, but trivial operations, error is bigger, and is easy not as good as flame photometry.
The method of enzymatic assays potassium content, with flare photometer or ion selective electrode method consistance is preferably arranged all, this method strong interference immunity, good stability, but can not measure potassium ion and sodion content separately simultaneously with Biochemical Analyzer, cause this method not applied.
Summary of the invention
The objective of the invention is: a kind of method that can overcome the enzymatic assays potassium content of prior art defective is provided, and uses the formulated potassium ion diagnostic kit of this method.Adopt the reagent in this kit, can utilize ultraviolet analyser or semi-automatic/automatic clinical chemistry analyzer to determine potassium content in the samples such as serum, blood plasma, the mensuration process is not subjected to the influence of sodion, finding speed is fast, accuracy is high, provides technical support for promoting enzyme process detection potassium content in clinical and the chemical analysis.
For realizing one of purpose of the present invention, a kind of method of enzymatic assays potassium content, adopt following steps to carry out:
At first,, make it to take place following reaction with needs sample of measuring and the reagent mixing that contains adenosine diphosphate, phosphoenolpyruvic acid, pyruvate kinase, lactic dehydrogenase and reduced coenzyme,
Then, reaction mixture is placed under ultraviolet analyser or the semi-automatic/automatic clinical chemistry analyzer, detect the decline rate of the absorbance of predominant wavelength 340nm, and then calculate the content of potassium ion in the sample.
In the mensuration process, the usage ratio of sample and reagent by volume was controlled at 1: 10～1: 500, and temperature of reaction is controlled at 20 ℃～50 ℃, and the reaction time was controlled at 2～30 minutes, set commplementary wave length during detection more than 405nm.
Realizing another one purpose of the present invention---potassium ion diagnostic kit, can be the single agent that is grouped into by following one-tenth:
Damping fluid 40～200mmol/l,
Adenosine diphosphate 1～10mmol/l,
Phosphoenolpyruvic acid 1～5mmol/l,
Reduced coenzyme 0.2～0.3mmol/l,
Pyruvate kinase 4000～20000U/l,
Lactic dehydrogenase 10 000～50000U/l,
Stabilizing agent/reagent cumulative volume 10～80%.
Also the various compositions in above single agent can be carried out formulated in combination and become two agent, be beneficial to eliminate inside and outside source pyruvic acid and pollute, such as:
Two agent prescriptions are not limited only in the above-mentioned table listed, and wherein the composition reduced coenzyme of reagent I and lactic dehydrogenase can be placed on reagent II; Adenosine diphosphate among the reagent II, phosphoenolpyruvic acid and pyruvate kinase also can be put into reagent I, so can form multiple formulations, enumerate no longer one by one.
Reagent can also be made into following three reagent, not only more help eliminating inside and outside source pyruvic acid and pollute, it is more stable to also help reagent:
| Agent III | Pyruvate kinase | 4000～20000U/l |
| Stabilizing agent/reagent III cumulative volume | 10～80％ |
Similar with two agent, three agent prescriptions also are not limited only to above-mentioned prescription, wherein reduced coenzyme among the reagent I and lactic dehydrogenase can be placed among reagent II or the reagent III, adenosine diphosphate among the reagent II and phosphoenolpyruvic acid can be placed among reagent I or the reagent III, pyruvate kinase among the reagent III also can be put among reagent I or the reagent II, so can form multiple formulations, enumerate no longer one by one.
When more than preparing the potassium ion diagnostic kit, selecting the basic demand of damping fluid is that pH value is within 6.0～11.0 scopes, can be: " trishydroxymethylaminomethane～hydrochloric acid (Tris-HCl) damping fluid ", " triethanolamine (Triethanolamine) damping fluid ", " 2-amino-2-methyl-1-propanol (2-Amino-2-methyl-1-propanol) damping fluid ", " imidazoles～hydrochloric acid (Imidazole-HCl) damping fluid ", " glycylglycine (Glycylglycine) damping fluid ", " citric acid～sodium citrate buffer solution ", " barbital sodium～hydrochloride buffer ", " sodium carbonate～sodium bicarbonate buffer liquid ", " boric acid～borate buffer solution ", " glycocoll～sodium hydrate buffer solution ", " borax～sodium hydrate buffer solution ", at least a in " sodium phosphate (Sodium Phosphate) damping fluid " or " phosphate (Phosphate-Sodium Chloride; PBS) damping fluid ", but the range of choice of damping fluid be not subjected to these enumerate limit.
In addition, for reducing the cross influence between each reagent composition, the stability of maintenance reagent, so that standing storage, usually add stabilizing agent in the middle of the reagent I/ reagent II of above single agent, two agent, three doses the reagent I/ reagent II/ reagent III, its consumption accounts for 10～80% (perhaps concentration is within 10～50mmol/l scopes) of place reagent cumulative volume.Material with used as stabilizers can be: at least a in ethylene glycol, propylene glycol, glycerine, ammonium sulfate, thioglycol, glucose, adenosine diphosphate, bovine serum albumin(BSA), carbonate, cholate, glucosan, ethylenediamine tetraacetic acid, flavin adenine dinucleotide (FAD), flavin mononucleotide (FMN), glutamate, reduced glutathione, lactose, sweet mellow wine, succinate or the sodium chloride, but range of choice be not subjected to equally these enumerate limit.
In the middle of the composition of above-mentioned diagnostic kit, described oxidized coenzyme is---oxidized form two nucleoside of nicotinamide adenine (NAD
+), NADP+ (NADP
+), thio-NAD+ (thio-NAD
+) or thio-NADP+ (thio-NADP
+); Described reduced coenzyme is---nicotinamide adenine dinucleotide reduced (NADH), NADPH (NADPH), Thio-NADH (thio-NADH) or thio-NADPH (thio-NADPH).
No matter studies show that, take all factors into consideration from the accuracy of measurement result and economy two aspects of preparation cost, be single agent, two agent or three doses, and the diagnostic kit of following formula components relation is comparatively desirable, also is preferred version of the present invention:
Damping fluid 80～120mmol/l,
Adenosine diphosphate 2～8mmol/l,
Phosphoenolpyruvic acid 1～5mmol/l,
Reduced coenzyme 0.2～0.3mmol/l,
Pyruvate kinase 6000～12000U/l,
Lactic dehydrogenase 10 000～30000U/l,
Stabilizing agent/reagent cumulative volume 10～50%.
General technical route of the present invention belongs to the content of pyruvate kinase coupling lactic dehydrogenase enzyme reaction colorimetric method for determining potassium ion, be specially: utilize potassium ion to intensify the characteristic of pyruvate kinase, make the reaction of adenosine diphosphate and phosphoenolpyruvic acid generate pyruvic acid, pyruvic acid generates lactic acid with the lactic dehydrogenase enzyme reaction again, simultaneously reduced coenzyme is oxidized to oxidized coenzyme.Because the degree that the pyruvate kinase activity intensifies is directly proportional with the content of potassium ion in the sample, and reduced coenzyme has very significantly absorption peak at 340nm, the oxidized coenzyme of their correspondences does not have absorption peak at 340nm, therefore, the variation of the absorbance that produced of reaction is directly proportional with the content of potassium ion in the sample.Like this, by in Fixed Time Interval, measuring the decline rate of predominant wavelength 340nm place absorbance, just can quantitative response go out the content of potassium ion in the sample.
The outstanding substantive distinguishing features and the obvious improvement of technical solution of the present invention mainly shows:
(1) the present invention utilizes Enzymology method fully, enzyme digestion reaction has the high characteristics of specificity, and by reduced coenzyme is reacted into oxidized coenzyme, quantitative response goes out the content of potassium ion in the sample, test result is accurate, is not subjected to whether to exist in the sample influence of sodion;
(2) composition of participation enzyme coupling reaction all adds, and is not subjected to the pollution of inside and outside source material, test process degree of accuracy height;
(3) this method is easy, easy to operate, can obtain testing result fast, and the reaction be under buffer conditions, to carry out, do not pollute the environment;
(4) but this method just fast detecting on general ultraviolet/visible light analysis instrument or semi-automatic/automatic clinical chemistry analyzer, do not need special or additional instruments, testing cost is cheap, be convenient to apply in the industry, be expected in clinical and chemical analysis, to replace flare photometer, make potassium content become the conventional analysis project;
(5) use the reagent that assay method provided by the invention can be made various ways such as liquid reagent, powdered reagent, dried reagent, be mainly used in the content of measuring potassium ion in human body and other animal body, also can be, supplementary means such as concentrate and measure potassium ion in other sample in conjunction with dilution;
(6) liquid potassium ion diagnostic kit provided by the invention, good stability, the three-D space structure that is present in wherein each kind of enzyme is kept perfectly, and has guaranteed the application testing effect well.Be made into after two agent or three doses, can further reduce the cross influence between the various compositions, testing result is more credible, and reagent is more stable, can store for a long time.
Embodiment
Below in conjunction with specific embodiment technical solution of the present invention is described further.These examples only are some exemplary applications, can not be interpreted as a kind of restriction to claim protection domain of the present invention.
Embodiment one (single agent)
Prepare the potassium ion diagnostic kit by following composition and consumption:
Glycylglycine damping fluid 80mmol/l,
Adenosine diphosphate 2mmol/l,
Phosphoenolpyruvic acid 1mmol/l,
thio-NADH 0.2mmol/l，
Pyruvate kinase 6000U/l,
Lactic dehydrogenase 10 000U/l,
Ethylene glycol 50% (accounting for the reagent cumulative volume).
Go up to set at automatic clinical chemistry analyzer (Hitachi-7080): 37 ℃ of temperature, 10 minutes reaction time, test predominant wavelength 340nm, more than the test commplementary wave length 405nm, the volume ratio of tested potassium ion sample and reagent is 1: 25, the Direction of Reaction is negative reaction (absorbance descends, down together).
Adding sample and the single agent that is made into, both detect, write down the decline situation of 340nm absorbance at the inner mixing automatically of analyser.According to methods such as rate method, end-point methods, the contrast corresponding standard curve calculates the content of potassium ion in the sample.Same batch sample is repeatedly repeated above process, test result good reproducibility, degree of accuracy height.
Embodiment two (two agent)
Prepare the potassium ion diagnostic kit by following composition and consumption:
Reagent I---
Imidazoles～hydrochloride buffer 100mmol/l,
NADPH 0.25mmol/l，
Lactic dehydrogenase 20000U/l,
Glycerine 50% (accounting for reagent I cumulative volume);
Reagent II---
Imidazoles～hydrochloride buffer 100mmol/l,
Adenosine diphosphate 5mmol/l,
Phosphoenolpyruvic acid 3mmol/l,
Pyruvate kinase 10000U/l,
Ethylene glycol 50% (accounting for reagent II cumulative volume).
Go up to set at automatic clinical chemistry analyzer (Hitachi-7080): 30 ℃ of temperature, 15 minutes reaction time, test predominant wavelength 340nm, more than the test commplementary wave length 405nm, tested potassium ion sample is 1: 25 with the ratio of the cumulative volume of reagent I and reagent II, reagent I is 4: 1 with the ratio of the consumption of reagent II, and the Direction of Reaction is negative reaction.
Adding sample and reagent I add reagent II after 5 minutes earlier, and the three detects, writes down the decline situation of 340nm absorbance at the inner mixing automatically of analyser.According to methods such as rate method, end-point methods, the contrast corresponding standard curve calculates the content of potassium ion in the sample.Same batch sample is repeatedly repeated above process, test result good reproducibility, degree of accuracy height.
Embodiment three (three doses)
Prepare the potassium ion diagnostic kit by following composition and consumption:
Reagent I---
Triethanolamine damping fluid 120mmol/l,
thio-NADPH 0.3mmol/l，
Lactic dehydrogenase 30000U/l,
Propylene glycol 50% (accounting for reagent I cumulative volume);
Reagent II---
Triethanolamine damping fluid 120mmol/l,
Adenosine diphosphate 8mmol/l,
Phosphoenolpyruvic acid 5mmol/l,
Propylene glycol 20mmol/l;
Reagent III---
Triethanolamine damping fluid 120mmol/l,
Pyruvate kinase 12000U/l,
Ethylene glycol 50% (accounting for reagent III cumulative volume).
Go up to set at automatic clinical chemistry analyzer (Hitachi-7080): 25 ℃ of temperature, 20 minutes reaction time, test predominant wavelength 340nm, more than the test commplementary wave length 405nm, tested potassium ion sample is 1: 25 with the ratio of the cumulative volume of reagent I, reagent II and reagent III, the consumption of reagent I, reagent II and reagent III is 8: 1: 1, and the Direction of Reaction is negative reaction.
Adding sample and reagent I, reagent II add reagent III after 5 minutes earlier, and they detect, write down the decline situation of 340nm absorbance at the inner mixing automatically of analyser.According to methods such as rate method, end-point methods, the contrast corresponding standard curve calculates the content of potassium ion in the sample.Same batch sample is repeatedly repeated above process, test result good reproducibility, degree of accuracy height.
Embodiment four (two agent preference)
Prepare the potassium ion diagnostic kit by following composition and consumption:
Reagent I---
Tris-HCl damping fluid 100mmol/l,
Adenosine diphosphate 2mmol/l,
Phosphoenolpyruvic acid 2mmol/l,
NADH 0.3mmol/l，
Lactic dehydrogenase 16000U/l,
Glycerine 50% (accounting for reagent I cumulative volume);
Reagent II---
Tris-HCl damping fluid 100mmol/l,
Pyruvate kinase 10000U/l,
Glycerine 50% (accounting for reagent II cumulative volume).
Go up to set at automatic clinical chemistry analyzer (Hitachi-7080): 37 ℃ of temperature, 10 minutes reaction time, test predominant wavelength 340nm, more than the test commplementary wave length 405nm, sample is 1: 25 with the ratio of the cumulative volume of reagent I and reagent II, reagent I is 4: 1 with the ratio of the consumption of reagent II, and the Direction of Reaction is negative reaction.
Add sample and reagent I earlier, add reagent II after 5 minutes, following principle reaction takes place at the inner mixing automatically of analyser in the three:
Detect, write down the decline situation of 340nm absorbance.According to methods such as rate method, end-point methods, the contrast corresponding standard curve calculates the content of potassium ion in the sample.Same batch sample is repeatedly repeated above process, test result good reproducibility, degree of accuracy height.
One of striking features of the present invention is the pollution that can eliminate inside and outside source pyruvic acid.The effect of eliminating inside and outside source pyruvic acid occurs in the first half of entire reaction time period, in time second half section, contaminated inside and outside source pyruvic acid is consumed totally, and the needed pyruvic acid of second half section time detecting all is to be derived from the sample potassium ion to the effect that intensifies of pyruvate kinase activity.
In a word, experimental results show that and adopt assay method of the present invention, can pass through ultraviolet analyser or semi-automatic/automatic clinical chemistry analyzer device fully, determine the content of the middle potassium ion of sample, measurement sensitivity height, degree of accuracy are good, be not subjected to whether to exist in the sample influence of sodion, be not subjected to the pollution of inside and outside source pyruvic acid yet.And, potassium ion diagnostic kit provided by the invention, good stability, long storage time still can accurately detect the content of potassium ion in all kinds sample afterwards.
Claims (9)
1. the method for an enzymatic assays potassium content may further comprise the steps:
1. with testing sample and the reagent mixing that contains adenosine diphosphate, phosphoenolpyruvic acid, pyruvate kinase, lactic dehydrogenase and reduced coenzyme, make it to take place following reaction,
2. reaction mixture is placed under ultraviolet analyser or the semi-automatic/automatic clinical chemistry analyzer, detect the decline rate of the absorbance of predominant wavelength 340nm, calculate the content of potassium ion in the sample.
2. the method for enzymatic assays potassium content according to claim 1 is characterized in that: the usage ratio of testing sample and reagent by volume was controlled at 1: 10～1: 500.
3. the method for enzymatic assays potassium content according to claim 1 and 2 is characterized in that: temperature of reaction is controlled at 20 ℃～50 ℃, and the reaction time was controlled at 2～30 minutes, sets commplementary wave length during detection more than 405nm.
4. potassium ion diagnostic kit, reagent is grouped into by following one-tenth in the box:
Damping fluid 40～200mmol/l,
Adenosine diphosphate 1～10mmol/l,
Phosphoenolpyruvic acid 1～5mmol/l,
Reduced coenzyme 0.2～0.3mmol/l,
Pyruvate kinase 4000～20000U/l,
Lactic dehydrogenase 10 000～50000U/l,
Stabilizing agent/reagent cumulative volume 10～80%.
5. potassium ion diagnostic kit according to claim 4 is characterized in that: the PH scope of described damping fluid is 6.0～11.0.
6. potassium ion diagnostic kit according to claim 5 is characterized in that: described damping fluid is " trishydroxymethylaminomethane～hydrochloride buffer ", " triethanolamine damping fluid ", " 2-amino-2-methyl-1-propanol damping fluid ", " imidazoles～hydrochloride buffer ", " glycylglycine damping fluid ", " citric acid～sodium citrate buffer solution ", " barbital sodium～hydrochloride buffer ", " sodium carbonate～sodium bicarbonate buffer liquid ", " boric acid～borate buffer solution ", " glycocoll～sodium hydrate buffer solution ", " borax～sodium hydrate buffer solution ", at least a in " sodium phosphate buffer " or " phosphate buffer ".
7. potassium ion diagnostic kit according to claim 4 is characterized in that: described stabilizing agent is at least a in ethylene glycol, propylene glycol, glycerine, ammonium sulfate, thioglycol, glucose, adenosine diphosphate, bovine serum albumin(BSA), carbonate, cholate, glucosan, ethylenediamine tetraacetic acid, flavin adenine dinucleotide (FAD), flavin mononucleotide (FMN), glutamate, reduced glutathione, lactose, sweet mellow wine, succinate or the sodium chloride.
8. potassium ion diagnostic kit according to claim 4 is characterized in that: described oxidized coenzyme is---NAD NAD
+, NADP+ NADP
+, thio-NAD+ thio-NAD
+Perhaps thio-NADP+ thio-NADP
+Described reduced coenzyme is---nicotinamide adenine dinucleotide reduced NADH, NADPH NADPH, Thio-NADH thio-NADH or thio-NADPH thio-NADPH.
9. any one potassium ion diagnostic kit in the claim 4～8 is characterized in that: described reagent is made into single agent, two agent or three doses.
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|---|---|---|---|
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|---|---|---|---|
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103175795A (en) * | 2011-12-20 | 2013-06-26 | 四川科伦药物研究有限公司 | Method for measuring content of potassium element in hydroxyethyl starch injection preparation |
| CN105420344A (en) * | 2015-12-12 | 2016-03-23 | 山东博科生物产业有限公司 | Stable serum-potassium detection reagent with high anti-interference capability and detection method |
| CN105784699A (en) * | 2016-04-25 | 2016-07-20 | 山东博科生物产业有限公司 | Potassium ion reagent with better stability and higher sensitivity and detection method |
| CN111208176A (en) * | 2020-01-15 | 2020-05-29 | 湖南工业大学 | Electrochemical test strip for detecting potassium ion and sodium ion concentration and detection method thereof |
-
2004
- 2004-12-10 CN CN 200410066183 patent/CN1786692A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103175795A (en) * | 2011-12-20 | 2013-06-26 | 四川科伦药物研究有限公司 | Method for measuring content of potassium element in hydroxyethyl starch injection preparation |
| CN105021557A (en) * | 2011-12-20 | 2015-11-04 | 四川科伦药物研究院有限公司 | Method for measuring content of potassium elements in hydroxyethyl starch injection preparation |
| CN105420344A (en) * | 2015-12-12 | 2016-03-23 | 山东博科生物产业有限公司 | Stable serum-potassium detection reagent with high anti-interference capability and detection method |
| CN105784699A (en) * | 2016-04-25 | 2016-07-20 | 山东博科生物产业有限公司 | Potassium ion reagent with better stability and higher sensitivity and detection method |
| CN111208176A (en) * | 2020-01-15 | 2020-05-29 | 湖南工业大学 | Electrochemical test strip for detecting potassium ion and sodium ion concentration and detection method thereof |
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