CN1778955A - Determination of inorganic phosphorus and its diagnostic reagent kit - Google Patents

Determination of inorganic phosphorus and its diagnostic reagent kit Download PDF

Info

Publication number
CN1778955A
CN1778955A CN 200410065576 CN200410065576A CN1778955A CN 1778955 A CN1778955 A CN 1778955A CN 200410065576 CN200410065576 CN 200410065576 CN 200410065576 A CN200410065576 A CN 200410065576A CN 1778955 A CN1778955 A CN 1778955A
Authority
CN
China
Prior art keywords
reagent
inorganic phosphorus
thio
damping fluid
diagnosis kit
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN 200410065576
Other languages
Chinese (zh)
Inventor
王尔中
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Suzhou ANJ Biotech Co Ltd
Original Assignee
Suzhou ANJ Biotech Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Suzhou ANJ Biotech Co Ltd filed Critical Suzhou ANJ Biotech Co Ltd
Priority to CN 200410065576 priority Critical patent/CN1778955A/en
Publication of CN1778955A publication Critical patent/CN1778955A/en
Pending legal-status Critical Current

Links

Landscapes

  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention is about the measuring method of Inorganic Phosphates and its diagnosis reagent box. Producing hydroperoxide by reacting pyruvate oxidase with pyruvate under the activation ofInorganic Phosphates in the sample of plasma or serum and so on, and producing acetaldehydeby reacting hydroperoxide with alcohol under the existence of hydroperoxide enzyme, and then transferring oxidized coenzyme to reduced coenzyme by reacting acetaldehyde and aldehyde dehydrogenase, testing the ascending range of dominant wave-length34nm absorbance before and after the reaction and finally measuring the content of Inorganic Phosphates. This method has high specificity and would not be contaminated by material of internal and exogenous sources, and the result is precise and accurate. Diving the diagnosis reagent box into double-dose or three-dose can reduces the cross interaction of each element, keeps the stability of the reagent and deposits chronically. Using this method can realize the fast testing in common ultraviolet/ visible light analyzer or semiautomatic/automatic analyzer and doesn't require special or additional apparatus, so the cost is low. Thus, this method can be easily promoted and applied in the whole industry.

Description

The measuring method of inorganic phosphorus and diagnostic kit thereof
Technical field
The present invention relates to measure the method for inorganic phosphorus, and use the formulated diagnostic kit of this method, belong to medical test determination techniques field.
Background technology
The phosphoric of inside of human body has 87% to be present in the bone approximately, and phosphorus in the blood and the phosphorus in the bone keep dynamic equilibrium relation.On the other hand, certain relation is arranged between the concentration of calcium and phosphorus in the blood, both products keep within limits, are unit with the milligram, and approximately the amassing of the content of calcium and phosphorus is 35~40 in per 100 milliliters of blood plasma.Normal people's blood calcium serium inorganic phosphorus that raises then reduces, and vice versa.When above-mentioned product greater than 40 the time, calcium and phosphorus are deposited on osseous tissue with the bone salts form; Greater than 70 o'clock, metastatic calcification can take place; Less than 35 o'clock, then will hinder the calcification of osseous tissue, even bone salts is dissolved again, influence osteogenesis, cause rickets or richets.Usually, can wait the concentration of regulating calcium and phosphorus in the blood plasma by vitamins D, Rat parathyroid hormone 1-34, thyrocalcitonin.
At present, the phosphorous total amount of human body can't directly be measured, and is that phosphorus acid ion concentration reflects in detection blood plasma or the serum mostly.Because H 2PO 4 -Can stable existence under natural condition such as blood, be the main existence form of inorganic phosphorus, thereby measure H 2PO 4 -Concentration just can represent or know by inference the total amount of inorganic phosphorus indirectly.Detection method commonly used has: phospho-molybdic acid reduction method/non-reduced method, dye binding method, enzyme process, isotopic dilution mass spectrometry, atomic absorption spectrophotometry, flow injection analysis or the like.Wherein, conclusive method is an isotopic dilution mass spectrometry, and the ordinary method that medium laboratory recommends to use is the phospho-molybdic acid colorimetry.
The phospho-molybdic acid reduction method is divided " no proteinemia filtrate method " and " not deproteinate method " again, and the former needs the Deproteinization with blood elder generation, and the latter needs to add nonionic surface active agent to avoid muddy in reagent.The kind of used reductive agent and tensio-active agent is a lot, and the more stable reductive agent of performance has ferrous sulfate, ferrous ammonium sulphate, hydrazonium sulfate, N-methyl p-aminophenol sulfate etc., and tensio-active agent is then the most desirable with polyethylene glycol groups phenyl ether (TritonX-100).
The non-reduced method of phospho-molybdic acid claims " direct ultraviolet method " again, is directly to measure the phospho-molybdic acid complex poly compounds at 340nm or 325nm wavelength, and method is easy, is convenient to automatization.But jaundice, haemolysis, the turbid serum of fat have absorption at 340nm, must do the sample blank, otherwise the result are higher.
Than other detection method, enzyme process is good, highly sensitive with its selectivity, can realize advantage such as automated analysis and enjoy people to favor, and is present people's research focus with the enzymatic assays content of inorganic phosphorus, also is development trend in the future.But, adopting different reagent and test route, the sensitivity of detection, the accuracy of detected result have very big difference, directly have influence on the use and the popularization of this method.
Summary of the invention
The objective of the invention is: a kind of method of enzymatic assays content of inorganic phosphorus is provided, and uses the formulated inorganic phosphorus diagnosis kit of this method.Adopt the reagent in this test kit, can utilize ultraviolet analyser or semi-automatic/automatic clinical chemistry analyzer that the content of inorganic phosphorus in the samples such as blood plasma, serum is carried out fast, accurately measures, so that promote the use of this detection method and diagnostic reagent, make in this area the medical test means of testing obtain abundant and development.
For realizing purpose of the present invention, a kind of method of measuring inorganic phosphorus, undertaken by following steps:
At first,, make it to take place following reaction with needs such as blood plasma, serum sample of measuring and the reagent mixing that contains pyruvic acid, pyruvic oxidase, ethanol, catalase, aldehyde dehydrogenase and oxidized coenzyme,
Then, reaction mixture is placed under ultraviolet analyser or half, the automatic clinical chemistry analyzer, detect the lift velocity of the absorbancy of predominant wavelength 340nm, and then calculate the content of inorganic phosphorus in the sample.
In the mensuration process, the usage ratio of sample and reagent by volume was controlled at 1: 10~1: 500, and temperature of reaction is controlled at 20 ℃~50 ℃, and the reaction times was controlled at 2~30 minutes, set commplementary wave length during detection more than 405nm.
Realize that it can be single agent that the inventive method is measured the diagnostic kit of content of inorganic phosphorus in the samples such as blood plasma, serum, is grouped into by following one-tenth:
Damping fluid 40~200mmol/l,
Pyruvic acid 1~30mmol/l,
Ethanol 1~20mmol/l,
Oxidized coenzyme 0.5~5mmol/l,
Pyruvic oxidase 1000~50000U/l,
Catalase 1000~50000U/l,
Aldehyde dehydrogenase 1000~50000U/l,
Stablizer/reagent cumulative volume 10~80%.
Also the various compositions in above single agent can be made up, be mixed with two agent, such as:
Composition Content range
Reagent I Damping fluid 40~200mmol/l
Ethanol 1~20mmol/l
Oxidized coenzyme 0.5~5mmol/l
Pyruvic oxidase 1000~50000U/l
Catalase 1000~50000U/l
Aldehyde dehydrogenase 1000~50000U/l
Stablizer/reagent I cumulative volume 10~80%
Reagent Damping fluid 40~200mmol/l
Pyruvic acid 1~30mmol/l
II Stablizer 10~50mmol/l
The prescription of two agent is not limited only in the above-mentioned table listed, the wherein composition of reagent I---ethanol, oxidized coenzyme, pyruvic oxidase, catalase, aldehyde dehydrogenase etc., can be placed on reagent II, pyruvic acid among the reagent II also can be put into reagent I, so can form multiple formulations, enumerate no longer one by one.
For making reagent more stable, can also be mixed with three doses, such as:
Composition Content range
Reagent I Damping fluid 40~200mmol/l
Ethanol 1~20mmol/l
Oxidized coenzyme 0.5~5mmol/l
Stablizer 10~50mmol/l
Reagent II Damping fluid 40~200mmol/l
Pyruvic oxidase 1000~50000U/l
Catalase 1000~50000U/l
Aldehyde dehydrogenase 1000~50000U/l
Stablizer/reagent II cumulative volume 10~80%
Reagent III Damping fluid 40~200mmol/l
Pyruvic acid 1~30mmol/l
Stablizer 10~50mmol/l
Similar with two agent, three doses prescription also is not limited only to above-mentioned prescription, wherein the ethanol of reagent I, oxidized coenzyme can be placed among reagent II or the reagent III, pyruvic oxidase among the reagent II, catalase, aldehyde dehydrogenase etc. can be placed among reagent I or the reagent III, pyruvic acid among the reagent III also can be put among reagent I or the reagent II, so can form multiple formulations, enumerate no longer one by one.
In the middle of the composition of above-mentioned diagnostic kit, selecting the basic demand of damping fluid is that pH value is within 6.0~11.0 scopes, can be: " Tutofusin tris~hydrochloric acid (Tris-HCl) damping fluid ", " trolamine (Triethanolamine) damping fluid ", " 2-amino-2-methyl-1-propanol (2-Amino-2-methyl-1-propanol) damping fluid ", " imidazoles~hydrochloric acid (Imidazole-HCl) damping fluid ", " glycylglycine (Glycylglycine) damping fluid ", " citric acid~sodium citrate buffer solution ", " Veronal sodium~hydrochloride buffer ", " boric acid~borate buffer solution ", " glycine~sodium hydrate buffer solution ", at least a in " borax~sodium hydrate buffer solution " or " yellow soda ash~sodium bicarbonate buffer liquid ", but range of choice be not subjected to these enumerate limit.
In addition, for reducing the cross influence between each reagent composition, the stability of maintenance reagent, so that standing storage, usually add stablizer in the middle of the reagent I/ reagent II of above single agent, two agent, three doses the reagent I/ reagent II/ reagent III, its consumption accounts for 10~80% (perhaps concentration is within 10~50mmol/l scopes) of place reagent cumulative volume.
Material with used as stabilizers can be: at least a in ethylene glycol, propylene glycol, glycerine, ammonium sulfate, thioglycol, adenosine diphosphate (ADP), bovine serum albumin, carbonate, cholate, dextran, ethylenediamine tetraacetic acid (EDTA), flavin adenine dinucleotide, vitamin B2 phosphate, glucose, glutaminate, reduced glutathion, lactose, N.F,USP MANNITOL, succinate or the sodium-chlor.
In the middle of the reagent composition of above-mentioned inorganic phosphorus diagnosis kit, described oxidized coenzyme is---NAD NAD +, NADP+ NADP +, thio-NAD+ thio-NAD +Perhaps thio-NADP+ thio-NADP +Described reduced coenzyme is---nicotinamide adenine dinucleotide reduced NADH, NADPH NADPH, Thio-NADH thio-NADH or thio-NADPH thio-NADPH.
No matter studies show that, take all factors into consideration from the accuracy of measurement result and economy two aspects of preparation cost, be single agent, two agent or three doses, and the inorganic phosphorus diagnosis kit of following system component relation is comparatively desirable, also is preferred version of the present invention:
Damping fluid 80~120mmol/l,
Pyruvic acid 4~10mmol/l,
Ethanol 2~8mmol/l,
Oxidized coenzyme 1~3mmol/l,
Pyruvic oxidase 10000~20000U/l,
Catalase 10000~20000U/l,
Aldehyde dehydrogenase 10000~20000U/l,
Stablizer/reagent cumulative volume 10~50%.
The present invention utilizes under the activation of the inorganic phosphorus of pyruvic oxidase in samples such as blood plasma, serum and generates hydrogen peroxide with the pyruvic acid effect, hydrogen peroxide generates acetaldehyde with ethanol synthesis under catalatic effect, acetaldehyde is reacted into reduced coenzyme with oxidized coenzyme again under the effect of aldehyde dehydrogenase.Because reduced coenzyme has very significantly absorption peak at 340nm, and the oxidized coenzyme of their correspondences does not have absorption peak at 340nm, therefore, measure the ascensional range of 340nm place, reaction front and back absorbancy, just can reflect the content of inorganic phosphorus in the sample well.
The outstanding substantive distinguishing features and the obvious improvement of technical solution of the present invention mainly shows:
(1) the present invention utilizes Enzymology method fully, and enzyme digestion reaction has the high characteristics of specificity, and by oxidized coenzyme is reacted into reduced coenzyme, quantitative response goes out the content of inorganic phosphorus in the sample, and test result is accurate;
(2) reacted constituent of participation enzyme linked reaction system all adds, and is not subjected to the pollution of inside and outside source material, test process tolerance range height;
(3) this method is easy, easy to operate, can obtain detected result fast, and the reaction be under buffer conditions, to carry out, do not pollute the environment;
(4) but this method just rapid detection on general ultraviolet/visible light analysis instrument or half, automatic clinical chemistry analyzer does not need special or additional instruments, testing cost is cheap, is convenient to apply in the industry;
(5) use the reagent that measuring method provided by the invention can be made various ways such as liquid reagent, powdered reagent, dried reagent, be mainly used in the content of measuring serium inorganic phosphorus in human body and other animal body, also can be, the content that supplementary means is measured inorganic phosphorus in other sample such as concentrate in conjunction with dilution;
(6) liquid inorganic phosphorus diagnosis kit provided by the invention, good stability, the three-D space structure that is present in wherein each kind of enzyme is kept perfectly, and has guaranteed the application testing effect well.Make after two agent or three doses, can further reduce the cross influence between the various compositions, improve the stability of reagent, be convenient to standing storage.
Embodiment
Below in conjunction with specific embodiment technical solution of the present invention is described further.These examples only are some exemplary applications, can not be interpreted as a kind of restriction to claim protection domain of the present invention.
Embodiment one (single agent)
Prepare inorganic phosphorus diagnosis kit in the serum by following composition and consumption:
Glycylglycine damping fluid 80mmol/l,
Pyruvic acid 4mmol/l,
Ethanol 2mmol/l,
thio-NAD + 1mmol/l,
Pyruvic oxidase 10000U/l,
Catalase 10000U/l,
Aldehyde dehydrogenase 10000U/l,
Ethylene glycol 50% (accounting for the reagent cumulative volume).
Go up to set at automatic clinical chemistry analyzer (Hitachi-7080): 37 ℃ of temperature, 10 minutes reaction times, test predominant wavelength 340nm, more than the test commplementary wave length 405nm, the volume ratio of sample and reagent is 1: 25, the Direction of Reaction is positive reaction (absorbancy rises, down together).
Adding serum sample and the single agent that is made into, both detect, write down the rising situation of 340nm absorbancy at the inner mixing automatically of analyser.Detect altogether and read a little 31 times, approximately every 20 seconds record one secondary data, according to methods such as rate method, end-point methods, the contrast corresponding standard curve calculates the content of inorganic phosphorus in the serum sample.Same batch sample is repeatedly repeated above process, test result good reproducibility, tolerance range height.
Embodiment two (two agent)
Prepare inorganic phosphorus diagnosis kit in the serum by following composition and consumption:
Reagent I---
Imidazoles~hydrochloride buffer 100mmol/l,
Ethanol 5mmol/l,
NADP + 2mmol/l,
Pyruvic oxidase 15000U/l,
Catalase 15000U/l,
Aldehyde dehydrogenase 15000U/l,
Glycerine 50% (accounting for reagent I cumulative volume);
Reagent II---
Imidazoles~hydrochloride buffer 100mmol/l,
Pyruvic acid 7mmol/l,
Ethylene glycol 50% (accounting for reagent II cumulative volume).
Go up to set at automatic clinical chemistry analyzer (Hitachi-7080): 30 ℃ of temperature, 15 minutes reaction times, test predominant wavelength 340nm, more than the test commplementary wave length 405nm, sample is 1: 25 with the ratio of the cumulative volume of reagent I and reagent II, reagent I is 4: 1 with the ratio of the consumption of reagent II, and the Direction of Reaction is positive reaction.
Adding serum sample and reagent I add reagent II after 5 minutes earlier, and the three detects, writes down the rising situation of 340nm absorbancy at the inner mixing automatically of analyser.According to methods such as rate method, end-point methods, the contrast corresponding standard curve calculates the content of inorganic phosphorus in the serum sample.Same batch sample is repeatedly repeated above process, test result good reproducibility, tolerance range height.
Embodiment three (three doses)
Prepare inorganic phosphorus diagnosis kit in the serum by following composition and consumption:
Reagent I---
Trolamine damping fluid 120mmol/l,
Ethanol 8mmol/l,
thio-NADP + 3mmol/l,
Propylene glycol 20mmol/l
Reagent II---
Trolamine damping fluid 120mmol/l,
Pyruvic oxidase 20000U/l,
Catalase 20000U/l,
Aldehyde dehydrogenase 20000U/l,
Propylene glycol 50% (accounting for reagent II cumulative volume);
Reagent III---
Trolamine damping fluid 120mmol/l,
Pyruvic acid 10mmol/l,
Ethylene glycol 20mmol/l.
Go up to set at automatic clinical chemistry analyzer (Hitachi-7080): 25 ℃ of temperature, 20 minutes reaction times, test predominant wavelength 340nm, more than the test commplementary wave length 405nm, sample is 1: 25 with the ratio of the cumulative volume of reagent I, reagent II and reagent III, the consumption of reagent I, reagent II and reagent III is 8: 1: 1, and the Direction of Reaction is positive reaction.
Adding serum sample and reagent I, reagent II add reagent III after 5 minutes earlier, and they detect, write down the rising situation of 340nm absorbancy at the inner mixing automatically of analyser.According to methods such as rate method, end-point methods, the contrast corresponding standard curve calculates the content of inorganic phosphorus in the serum sample.Same batch sample is repeatedly repeated above process, test result good reproducibility, tolerance range height.
Embodiment four (two agent preference)
Prepare the serium inorganic phosphorus diagnostic kit by following composition and consumption:
Reagent I---
Tris-HCl damping fluid 100mmol/l,
Pyruvic acid 10mmol/l,
Ethanol 4mmol/l,
NAD + 1mmol/l,
Glycerine 20mmol/l;
Reagent II---
Tris-HCl damping fluid 100mmol/l,
Pyruvic oxidase 10000U/l,
Catalase 15000U/l,
Aldehyde dehydrogenase 20000U/l,
Glycerine 50% (accounting for reagent II cumulative volume).
Go up to set at automatic clinical chemistry analyzer (Hitachi-7080): 37 ℃ of temperature, 10 minutes reaction times, test predominant wavelength 340nm, more than the test commplementary wave length 405nm, sample is 1: 25 with the ratio of the cumulative volume of reagent I and reagent II, reagent I is 4: 1 with the ratio of the consumption of reagent II, and the Direction of Reaction is positive reaction.
Add serum sample and reagent I earlier, add reagent II after 5 minutes, following principle reaction takes place at the inner mixing automatically of analyser in the three:
Detect, write down the rising situation of 340nm absorbancy.According to methods such as rate method, end-point methods, the contrast corresponding standard curve calculates the content of inorganic phosphorus in the serum sample.Same batch sample is repeatedly repeated above process, test result good reproducibility, tolerance range height.
In a word, experimental results show that and adopt measuring method of the present invention, can pass through ultraviolet analyser or semi-automatic/automatic clinical chemistry analyzer device fully, determine the content of inorganic phosphorus in the samples such as blood plasma, serum, measurement sensitivity height, tolerance range are good, are not subjected to the pollution of inside and outside source material.And, inorganic phosphorus diagnosis kit provided by the invention, good stability still can accurately detect the content of inorganic phosphorus in all kinds sample after the long storage time.

Claims (9)

1. method of measuring inorganic phosphorus may further comprise the steps:
1. with testing sample and the reagent mixing that contains pyruvic acid, pyruvic oxidase, ethanol, catalase, aldehyde dehydrogenase and oxidized coenzyme, make it to take place following reaction,
2. reaction mixture is placed under ultraviolet analyser or the semi-automatic/automatic clinical chemistry analyzer, detect the ascensional range of the absorbancy of predominant wavelength 340nm, calculate the content of inorganic phosphorus in the sample.
2. the method for mensuration inorganic phosphorus according to claim 1 is characterized in that: the usage ratio of testing sample and reagent by volume was controlled at 1: 10~1: 500.
3. the method for mensuration inorganic phosphorus according to claim 1 and 2 is characterized in that: temperature of reaction is controlled at 20 ℃~50 ℃, and the reaction times was controlled at 2~30 minutes, sets commplementary wave length during detection more than 405nm.
4. inorganic phosphorus diagnosis kit, reagent is grouped into by following one-tenth in the box:
Damping fluid 40~200mmol/l,
Pyruvic acid 1~30mmol/l,
Ethanol 1~20mmol/l,
Oxidized coenzyme 0.5~5mmol/l,
Pyruvic oxidase 1000~50000U/l,
Catalase 1000~50000U/l,
Aldehyde dehydrogenase 1000~50000U/l,
Stablizer/reagent cumulative volume 10~80%.
5. inorganic phosphorus diagnosis kit according to claim 4 is characterized in that: the PH scope of described damping fluid is 6.0~11.0.
6. inorganic phosphorus diagnosis kit according to claim 5 is characterized in that: described damping fluid is " Tutofusin tris~hydrochloride buffer ", " trolamine damping fluid ", " 2-amino-2-methyl-1-propanol damping fluid ", " imidazoles~hydrochloride buffer ", " glycylglycine damping fluid ", " citric acid~sodium citrate buffer solution ", " Veronal sodium~hydrochloride buffer ", " boric acid~borate buffer solution ", " glycine~sodium hydrate buffer solution ", at least a in " borax~sodium hydrate buffer solution " or " yellow soda ash~sodium bicarbonate buffer liquid ".
7. inorganic phosphorus diagnosis kit according to claim 4 is characterized in that: described stablizer is at least a in ethylene glycol, propylene glycol, glycerine, ammonium sulfate, thioglycol, adenosine diphosphate (ADP), bovine serum albumin, carbonate, cholate, dextran, ethylenediamine tetraacetic acid (EDTA), flavin adenine dinucleotide, vitamin B2 phosphate, glucose, glutaminate, reduced glutathion, lactose, N.F,USP MANNITOL, succinate or the sodium-chlor.
8. inorganic phosphorus diagnosis kit according to claim 4 is characterized in that: described oxidized coenzyme is---NAD NAD +, NADP+ NADP +, thio-NAD+ thio-NAD +Perhaps thio-NADP+ thio-NADP +Described reduced coenzyme is---nicotinamide adenine dinucleotide reduced NADH, NADPH NADPH, Thio-NADH thio-NADH or thio-NADPH thio-NADPH.
9. any inorganic phosphorus diagnosis kit in the claim 4~8 is characterized in that: described reagent is made into single agent, two agent or three doses.
CN 200410065576 2004-11-23 2004-11-23 Determination of inorganic phosphorus and its diagnostic reagent kit Pending CN1778955A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 200410065576 CN1778955A (en) 2004-11-23 2004-11-23 Determination of inorganic phosphorus and its diagnostic reagent kit

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 200410065576 CN1778955A (en) 2004-11-23 2004-11-23 Determination of inorganic phosphorus and its diagnostic reagent kit

Publications (1)

Publication Number Publication Date
CN1778955A true CN1778955A (en) 2006-05-31

Family

ID=36769410

Family Applications (1)

Application Number Title Priority Date Filing Date
CN 200410065576 Pending CN1778955A (en) 2004-11-23 2004-11-23 Determination of inorganic phosphorus and its diagnostic reagent kit

Country Status (1)

Country Link
CN (1) CN1778955A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101324613A (en) * 2007-06-13 2008-12-17 苏州艾杰生物科技有限公司 Inorganic phosphorus diagnosis/ determination reagent kit and method for determining inorganic phosphorus concentration
CN104819888A (en) * 2015-03-30 2015-08-05 中国农业科学院兰州兽医研究所 Sample diluting solution for ELISA, and preparation method thereof

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101324613A (en) * 2007-06-13 2008-12-17 苏州艾杰生物科技有限公司 Inorganic phosphorus diagnosis/ determination reagent kit and method for determining inorganic phosphorus concentration
CN104819888A (en) * 2015-03-30 2015-08-05 中国农业科学院兰州兽医研究所 Sample diluting solution for ELISA, and preparation method thereof
CN104819888B (en) * 2015-03-30 2018-01-09 中国农业科学院兰州兽医研究所 A kind of ELISA sample diluting liquids and preparation method thereof

Similar Documents

Publication Publication Date Title
CN1769481A (en) Blood ammonia content determination method and blood ammonia diagnosis kit
CN1778955A (en) Determination of inorganic phosphorus and its diagnostic reagent kit
CN1778953A (en) Determination of inorganic phosphorus and its diagnostic reagent kit
CN1778963A (en) Determination of blood ammonia content and blood ammonia diagnostic reagent kit
CN1778954A (en) Determination of inorganic phosphorus and its diagnostic reagent kit
CN1778941A (en) Determination of inorganic phosphorus and its diagnostic reagent kit
CN1778943A (en) Determination of inorganic phosphorus and its diagnostic reagent kit
CN1778956A (en) Determination of magnesium ion content from enzyme method and magnesium ion diagnostic reagent kit
CN1778960A (en) Determination of magnesium ion content from enzyme method and magnesium ion diagnostic reagent kit
CN1778962A (en) Determination of magnesium ion content from enzyme method and magnesium ion diagnostic reagent kit
CN1778958A (en) Determination of magnesium ion content from enzyme method and magnesium ion diagnostic reagent kit
CN1778957A (en) Determination of magnesium ion content from enzyme method and magnesium ion diagnostic reagent kit
CN1786692A (en) Process for determining content of potassium ion by enzyme method and kit for diagnosing potassium ion thereof
CN1778959A (en) Determination of magnesium ion content from enzyme method and magnesium ion diagnostic reagent kit
CN1778961A (en) Determination of magnesium ion content from enzyme method and magnesium ion diagnostic reagent kit
CN1778942A (en) Determination of inorganic phosphorus and its diagnostic reagent kit
CN1779464A (en) Determination of inorganic phosphorus and its diagnostic kit
CN1789427A (en) Monoamine oxidase activity determination method and monoamine oxidase diagnostic kit
CN1786187A (en) Enzymatic method for determining potassium ion content and potassium ion diagnosis kit
CN1778952A (en) Determination of inorganic phosphorus and its diagnostic reagent kit
CN1789428A (en) Monoamine oxidase activity determination method and monoamine oxidase diagnostic kit
CN1786694A (en) Process for determining content of carbon dioxide and kit for diagnosing carbon dioxide therefor
CN1786691A (en) Process for determining contect of potassium ion by enzyme method and kit for diagnosing potassium ion thereof
CN1302119C (en) Method for measuring 5'-nucleotidase activity and diagnostic reagent kit of 5'-nucleotidase
CN1789429A (en) Monoamine oxidase activity determination method and monoamine oxidase diagnostic kit

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C02 Deemed withdrawal of patent application after publication (patent law 2001)
WD01 Invention patent application deemed withdrawn after publication