CN1785348A - Compound Chinese medicinal preparation for treating type II diabetes and lowering blood sugar and its preparation method - Google Patents
Compound Chinese medicinal preparation for treating type II diabetes and lowering blood sugar and its preparation method Download PDFInfo
- Publication number
- CN1785348A CN1785348A CN 200510047792 CN200510047792A CN1785348A CN 1785348 A CN1785348 A CN 1785348A CN 200510047792 CN200510047792 CN 200510047792 CN 200510047792 A CN200510047792 A CN 200510047792A CN 1785348 A CN1785348 A CN 1785348A
- Authority
- CN
- China
- Prior art keywords
- rhizoma
- rhizoma paridis
- herba orostachyos
- preparation
- blood sugar
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
A hypoglycemic Chinese medicine for treating diabetes B is prepared from fimbriate orostachys herb and petiolata paris rhizome. Its preparing process is also disclosed. Its advantages are less loss of active components and high curative effect.
Description
Technical field
The present invention relates to medical technical field, relate to a kind of compound Chinese medicinal preparation that is used for the treatment of type ii diabetes, blood sugar lowering clinically and preparation method thereof specifically.
Background technology
Diabetes are the various Developmental and Metabolic Disorder syndromes based on hyperglycemia.Be broadly divided into two kinds on I type (insulin-dependent) and II type (insulin independent form) by the origin cause of formation.Type ii diabetes accounts for more than 95% of diabetics, because insulin secretion is low and insulin susceptibility lowly causes, beta Cell of islet is not destroyed, but the islets of langerhans number constantly reduces.There is the amyloid calmness at the islets of langerhans place.Be mainly in adult or old people, patient's onset is slower, and the state of an illness is lighter, and how fertile build is.The medicine of existing treatment diabetes mostly is oral and extensive use: sulfonylurea drugs such as glyburide, biguanides such as insoral, alpha-glucosidase inhibitor such as acarbose etc.But these medicines mostly are chemical synthetic drug, and the side effect of its clinical practice has report more, and are easy to generate drug resistance, and the patient just can keep curative effect using just must change dressings after a period of time kind of thing of certain medicine.
Summary of the invention
Purpose of the present invention is exactly the defective that has antidiabetic drug now in order to overcome, and a kind of medicine that is used for the treatment of type ii diabetes, hyperglycemia is provided, and its purpose is to improve the curative effect of medicine, reduces side effect.
The present invention takes new thinking, has changed in the past mostly with the means of chemical synthetic drug as the treatment diabetes, turns to and seek low, the eutherapeutic medicine of toxicity from natural product.Specifically, the present invention takes specific technology to obtain the effective extract compatibility from Herba Orostachyos and Rhizoma Paridis, produces synergism, thereby has realized the object of the invention, obtained a kind of evident in efficacy, the medicine that is used for the treatment of type ii diabetes, blood sugar lowering that toxic and side effects is little.
The present invention realizes that the purpose scheme just provides a kind of compound Chinese medicinal preparation for the treatment of type ii diabetes, blood sugar lowering, and composition comprises Herba Orostachyos and Rhizoma Paridis in the preparation.
The compound Chinese medicinal preparation of described treatment type ii diabetes, blood sugar lowering, main component is the mixture of Herba Orostachyos and Rhizoma Paridis in the preparation, is by weight proportion: Herba Orostachyos 1-3.5 part, Rhizoma Paridis 0.5-1.5 part, after writing out a prescription preferably, determine that its ratio is 2.5 parts of Herba Orostachyos, 1 part of Rhizoma Paridis.
The compound Chinese medicinal preparation of described treatment type ii diabetes, blood sugar lowering is made up of according to part by weight following raw material: Herba Orostachyos 1-4 part, Rhizoma Paridis 1-3 part, Fructus Schisandrae Chinensis 0.2-0.5 part; Radix Puerariae 0.3-0.5 part; Radix Panacis Quinquefolii 0.1-0.2 part.
The compound Chinese medicinal preparation of described treatment type ii diabetes, blood sugar lowering is made up of according to part by weight following raw material: Herba Orostachyos 3.5-1 part, Rhizoma Paridis 0.5-1.5 part, Radix Astragali 0.1-0.5 part; Radix Puerariae 0.3-0.5 part; Rhizoma Dioscoreae 0.1-0.4; Rhizoma Atractylodis 0.05-0.25.
The compound Chinese medicinal preparation of described treatment type ii diabetes, blood sugar lowering is made up of according to part by weight following raw material: Herba Orostachyos 5-1 part, Rhizoma Paridis 1.5-3 part, Rhizoma Anemarrhenae 6-8 part; Rhizoma Atractylodis 3-4 part.
The compound Chinese medicinal preparation of described treatment type ii diabetes, blood sugar lowering is made up of according to part by weight following raw material: Herba Orostachyos 5-1 part, Rhizoma Paridis 1-3 part, Rhizoma Coptidis 1-2 part; Radix Astragali 1-5 part; 2 parts of Spica Prunellaes; Rhizoma Dioscoreae 1-2 part; Rhizoma Zingiberis 0.5-1 part; Radix Paeoniae 0.5-1 part.
The compound Chinese medicinal preparation of described treatment type ii diabetes, blood sugar lowering is made up of according to part by weight following raw material: Herba Orostachyos 5-2 part, Rhizoma Paridis 1-5 part, Rhizoma Coptidis 6-9 part; Radix Et Rhizoma Rhei 1-3 part, Radix Glycyrrhizae 1-3 part: Poria 5-8 part, Rhizoma Atractylodis Macrocephalae 5-8 part, Rhizoma Polygonati 9-10 part.
Another object of the present invention is to provide a kind of preparation method of described compound Chinese medicinal preparation.
Pharmaceutic adjuvant in the compound Chinese medicinal preparation of the present invention is selected from: acceptable clinically bioadhesive material is such as chitin, chitosan; Disintegrating agent such as dried starch, carboxymethyl starch sodium or the low hydroxypropyl cellulose that replaces.
The preparation method of the compound Chinese medicinal preparation of treatment type ii diabetes of the present invention, blood sugar lowering comprises following processing step:
1. get the dry herb of Herba Orostachyos, coarse crushing adds alcohol reflux, filters, and medicinal residues wash with small amount of ethanol, merge extractive liquid, and cleaning mixture, and decompression recycling ethanol filters filtrate for later use to not having the alcohol flavor and concentrating;
2. the filtrate of gained in getting 1., spray drying, the powder-tight of gained is preserved;
3. get the dry medical material of Rhizoma Paridis, be ground into particulate, add the water reflux, extract,, filter, medicinal residues wash with low amounts of water, and merge extractive liquid, and cleaning mixture concentrate, and standing over night filters filtrate for later use;
4. the filtrate of gained in getting 3., spray drying, resulting powder-tight is preserved;
5. 3.5-1: the 0.5-1.5 effective ingredient of getting the orostachys fimbriatus effective component of said extracted and Rhizoma Paridis adds suitable pharmaceutic adjuvant and is prepared into Chinese traditional compound medicine of the present invention by weight proportion.
The invention still further relates to a kind of compound Chinese medicinal preparation for the treatment of type ii diabetes, blood sugar lowering that obtains by above-mentioned preparation method.
The compound Chinese medicinal preparation of preferred treatment type ii diabetes of the present invention, blood sugar lowering is to be prepared from by the following method:
1. get the dry herb of Herba Orostachyos and be ground into particulate, add 70% alcohol reflux three times, each 60 minutes of extraction time, filter, medicinal residues wash with small amount of ethanol, merge extractive liquid, and cleaning mixture, decompression recycling ethanol filters to not having the alcohol flavor and concentrating, and cold preservation is standby;
2. the filtrate of gained in getting 1., spray drying, the powder-tight of gained is preserved;
3. get Rhizoma Paridis and be ground into particulate, extracting in water three times, each 60 minutes of extraction time, filter, medicinal residues wash with low amounts of water, and merge extractive liquid, and cleaning mixture concentrate, and placement is spent the night, and filters filtrate for later use;
4. the filtrate of gained in getting 3., spray drying, resulting powder-tight is preserved;
5. 3.5-1: the 0.5-1.5 effective ingredient of getting the orostachys fimbriatus effective component of said extracted and Rhizoma Paridis adds suitable pharmaceutic adjuvant and is prepared into Chinese traditional compound medicine of the present invention by weight proportion.
Compound preparation of the present invention can be made various dosage forms, comprises capsule, tablet, granule etc., and medicine-feeding way is an oral administration.
Compound preparation of the present invention can be made capsule, and its preparation method is:
3.5-1: 0.5-1.5 gets the orostachys fimbriatus effective component that the present invention extracts and adds chitosan and L-HPC with the effective ingredient of Rhizoma Paridis by weight proportion, and mixing adds ethanol as binding agent, stirs granulation, treat the particle drying after, granulate is filled in the capsule shells promptly.The specification of this capsule is 250～350mg/ grain, usage: three times on the one, and each 2-3 grain.
Compound preparation of the present invention can also be made tablet, and its preparation method is:
3.5-1: 0.5-1.5 gets the orostachys fimbriatus effective component that the present invention extracts and adds chitosan and L-HPC mixing with the effective ingredient of Rhizoma Paridis by weight proportion, adds suitable binders system wet granular, drying, and granulate, the adding proper amount of lubricating agent, mixing, tabletting are promptly.The specification of this tablet is 0.2～0.3g/ sheet, usage: three times on the one, and each 2-3 sheet.
Compound preparation of the present invention can also be made granule, and its preparation method is:
3.5-1: 0.5-1.5 gets the orostachys fimbriatus effective component that the present invention extracts and adds chitosan, L-HPC and gas-producing disintegrant mixing with the effective ingredient of Rhizoma Paridis by weight proportion, is binding agent system soft material with ethanol, makes wet granular, loads in pouch, promptly after the drying.The specification of this granule is 0.4～0.5g/ bag, usage: three times on the one, and each one bag.
The used Herba Orostachyos of the present invention is the dry aerial parts of Crassulaceae plant Herba Orostachyos Orostachysfimbriatus (Turcz.) Berg., and sour in the mouth, hardship are cold in nature.Have cooling blood for hemostasis, heat-clearing and toxic substances removing, removing dampness and hold back effects such as skin ulcer.Record Herba Orostachyos removing heat from blood in the Tang Materia Medica can stop blooding and can promoting the circulation of blood end two-way function.Chinese scholars discovers that main component is flavone, oxalic acid etc. in the Herba Orostachyos.
Record in " Chinese medicine voluminous dictionary ", Herba Orostachyos is few medication.Pharmacological research is found now, and the water decoction that the dry herb of Herba Orostachyos is made to the Bufo siccus heart that exsomatizes, Cor Leporis room and Cor Leporis on the throne experiment, all has cardiotonic.Similar to the Electrocardiographic influence of Cavia porcellus to Ouabain.Experiment shows, the non-Ca of its cardiotonic
2+Institute extremely.Clinical research now shows that the Herba Orostachyos antiinflammatory action is good, in the treatment cervical erosion, and Herba Orostachyos group and matched group curative effect there was no significant difference, but method is simple, does not need special installation, does not damage health tissues, no cicatrix, no side reaction.
Pharmacological experiment shows, the ethanol extract of Herba Orostachyos 70% rises to the glucose load rat blood sugar the obvious suppression effect, and the rat administration blood glucose value and the matched group of normal blood glucose value do not had significant change; For the inductive diabetic mice of alloxan, can obviously suppress the rising of blood glucose after two weeks and can reduce the content of the triglyceride in the blood at oral administration.For having genetic KKAy diabetic mice, oral administration just shows after one week and suppresses blood glucose rising activity, and prolongs active further enhancing with administration time, and the while can also be suppressed the rising of triglyceride in the blood.Experiment in vitro shows that the ethanol extract of Herba Orostachyos has stronger aldose reductase inhibition activity.
Rhizoma Paridis is liliaceous plant Rhizoma Paridis Parispolyphylla Smithvar.yunnanensis (Franch.) Hand.-Mazz, or Rhizoma Paridis Paris polyphyllaSmith var, the dry rhizome of chihensis (Fanch.) Hara.Bitter in the mouth, cold in nature, slightly poisonous." (book on Chinese herbal medicine is asked the source " middle record, the king in the grass of Rhizoma Paridis position, its merit is held concurrently ginseng, fine and soft, three phases, adds medicine to for impairment caused by overstrain, controls pestilence, the subduing inflammation magical effect, the effect of cool liver arresting convulsion.About the report of the chemical constituent in the Rhizoma Paridis, report is arranged both at home and abroad repeatly, now therefrom isolate 50 surplus kind of chemical compound.Main effective ingredient is a steroidal saponin in the Rhizoma Paridis, accounts for 80% of total chemical compound number, and contains chemical compounds such as aminoacid, sterone, ecdyson, flavonoid glycoside, and its The Chemical Constituents was focused mostly on the eighties in 20th century, and relevant report is less in recent ten years.Modern pharmacology and clinical research show, the complex chemical composition of Rhizoma Paridis, and pharmacologically active is strong, and clinical application range is wide.
Pharmacological experiment shows, Rhizoma Paridis has the blood glucose that the excessive oral saccharic of remarkable inhibition causes and sharply rises, can to diabetes particularly the type ii diabetes patient back blood glucose of taking food rise to inhibitory action on sharply, reduce the damage of rising and causing successively because of blood glucose.Experiment in vitro shows that Rhizoma Paridis suppresses blood glucose rising activity to be caused owing to Rhizoma Paridis can suppress disaccharide compound hydrolysis absorption.
The present invention is mixed in proportion the active part that Herba Orostachyos and Rhizoma Paridis extract, because the mechanism of action of two kinds of activity extracts is different with target spot, with two kinds of active component use in conjunction, can produce synergism, improves curative effect, reduces incidence rate of adverse reaction.The present invention is intended to keep and formerly can reduces toxic and side effects powerful the time, thereby reduces the generation of untoward reaction.
Characteristics of the present invention are: with Herba Orostachyos, Rhizoma Paridis is the preparation raw material medical material, on the basis that Herba Orostachyos, Rhizoma Paridis effective ingredient physicochemical property are fully studied, extract refining according to every flavor medicine self character respectively by specific technology, keep effective ingredient to greatest extent, remove relative substance composition and the composition that causes side effect, the curative effect of medication that the present invention is made is remarkable, and toxic and side effects is low, for be used for the treatment of clinically type ii diabetes, blood sugar lowering provide new by way of.
The specific embodiment
The following example is in order further to describe the present invention for example, rather than limits the present invention by any way, and the following example all is prescriptions that patent applicant of the present invention has very good effect through a large amount of verification experimental verifications.The present invention will be not used in two kinds of medicine Herba Orostachyos controlling diabetes in the past and heavily hug and make up, and can also add the medicine that other crude drug preparation has treatment diabetes effect behind these two kinds of drug regimens.
Embodiment 1 (capsule):
1. get the dry herb 1kg of Herba Orostachyos, coarse crushing adds 70% ethanol 10L at every turn, and reflux, extract, three times filters, and medicinal residues wash with small amount of ethanol, merge extractive liquid, and cleaning mixture, and decompression recycling ethanol filters filtrate for later use to not having the alcohol flavor and being concentrated into 4L;
2. the filtrate of gained in getting 1., spray drying, the powder-tight of gained saves as orostachys fimbriatus effective component;
3. get Rhizoma Paridis 1kg and be ground into particulate, add water 30L and extract three times, each 60 minutes of extraction time, filter, medicinal residues wash with low amounts of water, and merge extractive liquid, and cleaning mixture are concentrated into 4L, and placement is spent the night, and filters, and filtrate for later use is the Rhizoma Paridis effective ingredient;
4. the filtrate of gained in getting 3., spray drying, resulting powder-tight is preserved;
5. 3.5-1: 0.5-1.5 gets the Herba Orostachyos that the present invention extracts and adds chitosan and L-HPC with the effective ingredient of Rhizoma Paridis by weight proportion, and mixing adds ethanol as binding agent, stirs granulation, treat the particle drying after, granulate is filled in No. 1 capsule shells promptly.
Measure by high performance liquid chromatography (appendix VD of Chinese Pharmacopoeia version in 2005), contain flavone (in Quercetin and kaempferide sum) in each capsules and must not be less than 2.89mg, Rhizoma Paridis saponin (in Rhizoma Paridis saponin I and II sum) must not be less than 1.43mg
Embodiment 2 (tablet):
1. get the dry herb 1kg of Herba Orostachyos, coarse crushing adds 70% ethanol 10L at every turn, and reflux, extract, three times filters, and medicinal residues wash with small amount of ethanol, merge extractive liquid, and cleaning mixture, and decompression recycling ethanol filters filtrate for later use to not having the alcohol flavor and being concentrated into 4L;
2. the filtrate of gained in getting 1., spray drying, the powder-tight of gained is preserved;
3. get Rhizoma Paridis 1kg and be ground into particulate, add water 30L and extract three times, each 60 minutes of extraction time, filter, medicinal residues wash with low amounts of water, and merge extractive liquid, and cleaning mixture are concentrated into 4L, and placement is spent the night, and filters, and filtrate for later use is the Rhizoma Paridis effective ingredient;
4. the filtrate of gained in getting 3., spray drying, resulting powder-tight is preserved;
5. 3.5-1: 0.5-1.5 gets the orostachys fimbriatus effective component that the present invention extracts and adds chitosan and L-HPC mixing with the effective ingredient of Rhizoma Paridis by weight proportion, adds suitable binders system wet granular, drying, and granulate, the adding proper amount of lubricating agent, mixing, tabletting are promptly.Every heavily about 0.3g.
Measure by high performance liquid chromatography (appendix VD of Chinese Pharmacopoeia version in 2005), contain flavone (in Quercetin and kaempferide sum) in each sheet and must not be less than 3mg, Rhizoma Paridis saponin (a Rhizoma Paridis saponin I and II sum meter) must not be less than 1.5mg
Embodiment 3 (granule):
1. get the dry herb 1kg of Herba Orostachyos, coarse crushing adds 70% ethanol 10L at every turn, and reflux, extract, three times filters, and medicinal residues wash with small amount of ethanol, merge extractive liquid, and cleaning mixture, and decompression recycling ethanol filters filtrate for later use to not having the alcohol flavor and being concentrated into 4L;
2. the filtrate of gained in getting 1., spray drying, the powder-tight of gained is preserved;
3. get Rhizoma Paridis 1kg and be ground into particulate, add water 30L and extract three times, each 60 minutes of extraction time, filter, medicinal residues wash with low amounts of water, and merge extractive liquid, and cleaning mixture are concentrated into 4L, and placement is spent the night, and filters filtrate for later use;
4. the filtrate of gained in getting 3., spray drying, resulting powder-tight is preserved;
5. 3.5-1: 0.5-1.5 gets the orostachys fimbriatus effective component that the present invention extracts and adds chitosan, L-HPC and gas-producing disintegrant mixing with the effective ingredient of Rhizoma Paridis by weight proportion, is binding agent system soft material with ethanol, makes wet granular, loads in pouch, promptly after the drying.The specification of this granule is 0.4～0.5g/ bag.
Measure by high performance liquid chromatography (appendix VD of Chinese Pharmacopoeia version in 2005), contain flavone (in Quercetin and kaempferide sum) in each bag preparation and must not be less than 5.91mg, Rhizoma Paridis saponin (a Rhizoma Paridis saponin I and II sum meter) must not be less than 2.88mg
Embodiment 4 (granule):
1. get the dry herb 2.5kg of Herba Orostachyos, coarse crushing adds 70% ethanol 10L at every turn, and reflux, extract, three times filters, and medicinal residues wash with small amount of ethanol, merge extractive liquid, and cleaning mixture, and decompression recycling ethanol filters filtrate for later use to not having the alcohol flavor and being concentrated into 4L;
2. the filtrate of gained in getting 1., spray drying, the powder-tight of gained is preserved, and is orostachys fimbriatus effective component;
3. get Rhizoma Paridis 1kg and be ground into particulate, add water 30L and extract three times, each 60 minutes of extraction time, filter, medicinal residues wash with low amounts of water, and merge extractive liquid, and cleaning mixture are concentrated into 4L, and placement is spent the night, and filters filtrate for later use;
4. the filtrate of gained in getting 3., spray drying, resulting powder-tight is preserved, and is the Rhizoma Paridis effective ingredient;
5. the orostachys fimbriatus effective component of getting 2. and 4. extracting adds chitosan, L-HPC and gas-producing disintegrant mixing with the effective ingredient of Rhizoma Paridis, is binding agent system soft material with ethanol, makes wet granular, loads in pouch, promptly after the drying.The specification of this granule is 0.4～0.5g/ bag.
Pharmacodynamics embodiment
Embodiment 1 (the Herba Orostachyos active site is to the blood sugar influence experiment of glucose load rat):
1 medicine and reagent
(1) active site of the Herba Orostachyos in the embodiment of the invention
(2) 5% gumwaters
(3) sucrose
(4) 5% glucose injections: Zhiying Pharmaceutical Factory, Shenyang
Lot number: 05030701
(5) ether
Lot number: F 20020802 chemical pure CP
Face with preceding and prepare with 0.9% normal saline
2 laboratory animals
Wistar rat body weight (180～220g) male and female half and half
Laboratory animal is provided by Shenyang Pharmaceutical University's Experimental Animal Center
The quality certification number: SCXK (the Liao Dynasty) 2003-008
3 experimental apparatus
(1) RM6240CD type multi-path physiology signal acquiring system Chengdu instrument maker
(2) balance equipment factory of company of Nereid section on the JA1003 electronic balance
(3) balance equipment factory of company of Nereid section on the JY8001 electronic balance
4 experimental techniques
To be tried rat and be divided into 5 groups at random, 10 every group, went on a hunger strike before the experiment 24 hours, freely drink water.Herba Orostachyos 70% ethanol extraction is suspended in 5% arabic gum, according to 5ml/kg dosage oral administration.Normal group and matched group only give 5% gumwater, and after the administration 30 minutes, the glucose of orally give 1.0g/kg, aqueous sucrose solution, normal group gives same dosage distilled water.0.5, following corner of the eyes vein of etherization got blood 0.4ml after 1,2 hour, blood sample is isolated blood plasma after 5000rpm is centrifugal, usefulness glucose oxidase method is through inspection amount line mensuration blood glucose value.
5 statistical procedures methods
Experimental data represents that with meansigma methods x ± standard deviation (SD) statistics are checked with T between group between group.Enumeration data statistics X
2Check.
6 results and discussion
The result is shown in table 1,2, and Herba Orostachyos 70% extract rises to the blood glucose of sucrose, glucose load rat the obvious suppression effect, and with the increase of dosage, suppresses active and constantly strengthen.
Table 1. Herba Orostachyos 70% ethanol extraction is to the influence of glucose load rat blood sugar value
| n | 0.5hr | 1hr | 2hr | |
| Blank group model group 137.5mg/kg 275mg/kg 550mg/kg | 10 10 10 10 10 | 97.1±2.0** 179.3±3.7 146.8±12.2* 139.8±16.1** 113.3±5.7** | 95.1±3.5** 158.1±7.2 147.3±13.0 125.8±13.4* 128.0±4.4* | 95.4±4.5** 121.3±8.2 123.7±5.8 115.7±16.2 117.1±2.7 |
Administration group and model group compare, * P＜0.05 * * P＜0.01
Table 2. Herba Orostachyos 70% ethanol extraction is to the influence of sucrose load rat blood sugar value
| n | 0.5hr | 1hr | 2hr | |
| Blank group model group 137.5mg/kg | 5 6 5 | 56.6±2.0** 166.3±3.7 146.8±12.2** | 71.3±3.5** 149.1±7.2 147.3±13.0 | 68.8±4.5** 121.3±8.2 123.7±5.8 |
| 275mg/kg 550mg/kg | 5 5 | 134.3±2.6* 121.9±3.0 | 140.5±13.4 132.0±4.4 | 115.7±16.2 117.1±2.7 |
Administration group and model group compare, * P＜0.05 * * P＜0.01
Embodiment 2 (the Herba Orostachyos active site is to the therapeutical effect of KKAy/Ta Jcl diabetic mice):
1 medicine and reagent
(1) active site of the Herba Orostachyos in the embodiment of the invention
(2) 5% gumwaters
(3) troglitazone: Shanghai Zhongxi Pharmaceutical Co., Ltd.
Lot number: 0410004
Consumption: adult's intravenous drip, a 10～16ml is with 5% glucose injection 100
～500ml dilution back is used 1 time on the one
2 laboratory animals
Male 5 the week age KKAy/Ta Jcl diabetic mice, body weight 23-28g,
Laboratory animal is provided by Shenyang Pharmaceutical University's Experimental Animal Center
The quality certification number: SCXK (the Liao Dynasty) 2003-008
3 experimental apparatus
(1) RM6240CD type multi-path physiology signal acquiring system Chengdu instrument maker
(2) balance equipment factory of company of Nereid section on the JA1003 electronic balance
(3) balance equipment factory of company of Nereid section on the JY8001 electronic balance
4 experimental techniques
After the KKAy diabetic mice was tamed through a week, according to the blood sugar level grouping, every afternoon, 5 oral administrations were not kept on a diet before the administration.In 2 weeks of successive administration, measure serum composition under the once non-state of going on a hunger strike weekly.Positive control drug is a troglitazone.
5 statistical procedures methods
Experimental data represents that with meansigma methods x ± standard deviation (SD) statistics are checked with T between group between group.Enumeration data statistics X
2Check.
6 results and discussion
The result is shown in table 3,4,5, and the Herba Orostachyos extract has tangible blood sugar lowering effect to the type ii diabetes model mice, though do not observe significant blood fat reducing effect, from experimental result as can be seen, Herba Orostachyos also has certain downward trend to blood fat.If administration time prolongs, this trend may be more obvious.
Table 3. Herba Orostachyos 70% ethanol extraction is to the influence of KKAy blood glucose in diabetic mice value
| Dose | n | Blood glucose levels(mg/100ml) |
| (mg/kg/day， p.o.) | 0week | 1week | 2week | ||
| Model group administration group positive controls | - 275 550 100 | 11 10 10 10 | 379.2±28.4 351.4±24.9 359.6±25.0 367.9±23.9 | 459.3±28.1 374.1±37.0 382.2±33.8 329.5±26.4* | 527.5±23.8 404.5±46.1* 374.7±32.2** 405.3±29.0* |
Administration group and model group compare, * P＜0.05 * * P＜0.01
Table 4. Herba Orostachyos 70% ethanol extraction is to the influence of triglyceride in the KKAy diabetic mice serum
| Dosage (mg/kg/day, p.o.) | Blood triglyceride levels(mg/100ml) | |||
| n | 1week | 2week | ||
| Model group administration group positive controls | - 275 550 100 | 11 10 10 10 | 339.3±14.2 347.1±22.7 294.2±27.4 287.5±19.0 | 524.8±21.4 451.1±39.2 451.4±41.2 429.5±50.3 |
Administration group and model group compare, * P＜0.05 * * P＜0.01
Table 5. Herba Orostachyos 70% ethanol extraction is to the influence of free fatty acid in the KKAy diabetic mice serum
| Dose (mg/kg/day， p.o.) | n | Blood free fatty acid levels (mEq/L) | ||
| 1week | 2week | |||
| Model group administration group positive controls | - 275 550 100 | 11 10 10 10 | 3.0±0.6 3.2±0.8 3.4±0.9 2.3±0.6 | 2.9±0.7 2.8±0.6 3.2±0.8 2.7±0.7 |
Administration group and model group compare, * P＜0.05 * * P＜0.01
Embodiment 3 (the Rhizoma Paridis active site rises to the glucose load rat blood sugar to be influenced):
1 medicine and reagent
(1) active site of the Rhizoma Paridis in the embodiment of the invention
(2) 5% gumwaters
(3) sucrose
(4) 5% glucose injections: Zhiying Pharmaceutical Factory, Shenyang
Lot number: 05030701
(5) ether
Lot number: F 20020802 chemical pure CP
Face with preceding and prepare with 0.9% normal saline
2 laboratory animals
Wistar rat body weight (180～220g) male and female half and half
Laboratory animal is provided by Shenyang Pharmaceutical University's Experimental Animal Center
The quality certification number: SCXK (the Liao Dynasty) 2003-008
3 experimental apparatus
(1) RM6240CD type multi-path physiology signal acquiring system Chengdu instrument maker
(2) balance equipment factory of company of Nereid section on the JA1003 electronic balance
(3) balance equipment factory of company of Nereid section on the JY8001 electronic balance
4 experimental techniques
To be tried rat and be divided into 5 groups at random, 10 every group, went on a hunger strike before the experiment 24 hours, freely drink water.The Rhizoma Paridis water extract is suspended in 5% arabic gum, according to 5ml/kg dosage oral administration.Normal group and matched group only give 5% gumwater, and after the administration 30 minutes, the aqueous sucrose solution of orally give 1.0g/kg, normal group gives same dosage distilled water.0.5, following corner of the eyes vein of etherization got blood 0.4ml after 1,2 hour, blood sample is isolated blood plasma after 5000rpm is centrifugal, usefulness glucose oxidase method is through inspection amount line mensuration blood glucose value, blood sugar test result is shown in table 1,2.
5 statistical procedures methods
Experimental data represents that with meansigma methods x ± standard deviation (SD) statistics are checked with T between group between group.Enumeration data statistics X
2Check.
6 results and discussion
The result is as shown in table 6, Rhizoma Paridis is the same with Herba Orostachyos, also have the blood glucose that the excessive oral saccharic of remarkable inhibition causes and sharply rise, can to diabetes particularly after type ii diabetes patient's the feed blood glucose rise to inhibitory action on sharply, therefore reduce the damage of rising and causing because of blood glucose.In conjunction with interior animal experiment result and glucose hydrolysis enzyme experimental result, determined that Rhizoma Paridis suppresses blood glucose rising activity and causes owing to Rhizoma Paridis can suppress disaccharide compound hydrolysis absorption.
Table 6. Rhizoma Paridis water extract is to the influence of sucrose load rat blood sugar value
| n | 0.5hr | 1hr | 2hr | |
| Blank group model group 55mg/kg 110mg/kg 220mg/kg | 6 6 6 6 6 | 89.1±3.3** 184.0±6.4 139.3±6.8** 104.3±3.1** 94.2±3.3** | 94.5±4.6** 155.0±5.4 135.2±4.6* 120.1±1.5** 110.0±5.4* | 94.1±3.1** 124.8±3.6 120.0±1.8 116.5±3.1 107.3±4.4** |
Administration group and model group compare, * P＜0.05 * * P＜0.01
Embodiment 4 (influence that Herba Orostachyos and Rhizoma Paridis combination drug rise to blood glucose):
1 medicine and reagent
(1) active site of the Rhizoma Paridis in the embodiment of the invention
(2) 5% gumwaters
(3) sucrose
(4) 5% glucose injections: Zhiying Pharmaceutical Factory, Shenyang
Lot number: 05030701
(5) ether
Lot number: F 20020802 chemical pure CP
2 laboratory animals
Wistar rat body weight (180～220g) male and female half and half
Laboratory animal is provided by Shenyang Pharmaceutical University's Experimental Animal Center
3 experimental apparatus
(1) RM6240CD type multi-path physiology signal acquiring system Chengdu instrument maker
(2) balance equipment factory of company of Nereid section on the JA1003 electronic balance
(3) balance equipment factory of company of Nereid section on the JY8001 electronic balance
4 experimental techniques
Suppress the effect that blood glucose rises because Herba Orostachyos and Rhizoma Paridis all have, but activity mechanism but there is significant difference, considers that therefore Rhizoma Paridis and Herba Orostachyos combination drug will produce stronger blood glucose rising and suppress active.
To be tried rat and be divided into 5 groups at random, 10 every group, went on a hunger strike before the experiment 24 hours, freely drink water.The Rhizoma Paridis water extract is suspended in 5% arabic gum, according to 5ml/kg dosage oral administration.Normal group and matched group only give 5% gumwater, and after the administration 30 minutes, the aqueous sucrose solution of orally give 1.0g/kg, normal group gives same dosage distilled water.0.5, following corner of the eyes vein of etherization got blood 0.4ml after 1,2 hour, blood sample is isolated blood plasma after 5000rpm is centrifugal, usefulness glucose oxidase method is through inspection amount line mensuration blood glucose value, blood sugar test result is shown in table 11,12.
According to former experimental result, adopt Herba Orostachyos and Rhizoma Paridis moderate to suppress blood glucose rising dosage, be respectively Herba Orostachyos maximal dose 275mg/kg, the Rhizoma Paridis maximal dose is 110mg/kg, change the proportioning of two kinds of medicines gradually, set up 5 administration groups altogether, be respectively Rhizoma Paridis 110mg/kg, (Rhizoma Paridis: Herba Orostachyos 100: 0), Rhizoma Paridis 82.5mg/kg+ Herba Orostachyos 68.75mg/kg (Rhizoma Paridis: Herba Orostachyos 75: 25), Rhizoma Paridis 55mg/kg+ Herba Orostachyos 137.5mg/kg (Rhizoma Paridis: Herba Orostachyos 50: 50), Rhizoma Paridis 27.5mg/kg+ Herba Orostachyos 206.25mg/kg (Rhizoma Paridis: Herba Orostachyos 25: 75), Herba Orostachyos 275mg/kg (Rhizoma Paridis: Herba Orostachyos 0: 100), the consistent unanimity effect of discussing the blood glucose rising with above experimental technique.
5 statistical procedures methods
Experimental data represents that with meansigma methods x ± standard deviation (SD) statistics are checked with T between group between group.Enumeration data statistics X
2Check.
6 results and discussion
The result is as shown in table 7, and Rhizoma Paridis and Herba Orostachyos are about at 1: 1 to 1: 3 o'clock in weight ratio, all can produce active facilitation, and the proportional quantity of Herba Orostachyos increases, and by about 80% o'clock, does not have active facilitation basically.
The inhibitory action that under the table 7. Rhizoma Paridis Herba Orostachyos different conditions of mixture ratios blood glucose is risen
| Dose (mg/kg，p.o.) | n | Blood glucose levels(mg/100ml) | |||
| 0.5hr | 1hr | 2hr | |||
| Blank group model group Rhizoma Paridis Rhizoma Paridis+Herba Orostachyos Herba Orostachyos | - - 110 82.5+68.57 55+137.5 27.5+206.25 275 | 5 6 5 5 5 5 6 | 63.2±7.3 168.9±2.8 110.8±19.9** 99.8±6.4** 101.9±6.6** 129.7±12.2** 133.1±5.0** | 64.8±6.0 147.6±4.6 106.5±14.3** 99.8±9.2** 100.7±14.7** 130.8±6.0 138.3±8.2 | 61.3±5.6 113.1±5.2 87.2±3.6 100.5±13.2 100.7±5.7 136.7±5.7 118.4±7.4 |
Administration group and model group compare, * P＜0.05 * * P＜0.01
Embodiment 5
The present embodiment medicine is made up of according to part by weight following raw material: 1 part of Herba Orostachyos, 1.5 parts of Rhizoma Paridis; Preparation method is with embodiment 4.
Embodiment 6
The present embodiment medicine is made up of according to part by weight following raw material: 3.5 parts of Herba Orostachyos, 0.5 part of Rhizoma Paridis; Preparation method is with embodiment 4.
Embodiment 7
The present embodiment medicine is made up of according to part by weight following raw material: 1 part of Herba Orostachyos, 3 parts of Rhizoma Paridis, 0.5 part of Fructus Schisandrae Chinensis; 0.5 part of Radix Puerariae; 0.2 part of Radix Panacis Quinquefolii.
It is prepared into capsule comprises following processing step:
1. get the dry herb 1kg of Herba Orostachyos, coarse crushing adds 70% ethanol 10L at every turn, and reflux, extract, three times filters, and medicinal residues wash with small amount of ethanol, merge extractive liquid, and cleaning mixture, and decompression recycling ethanol filters filtrate for later use to not having the alcohol flavor and being concentrated into 4L;
2. the filtrate of gained in getting 1., spray drying, the powder-tight of gained saves as orostachys fimbriatus effective component;
3. get Rhizoma Paridis 3kg and be ground into particulate, add water 30L and extract three times, each 60 minutes of extraction time, filter, medicinal residues wash with low amounts of water, and merge extractive liquid, and cleaning mixture are concentrated into 4L, and placement is spent the night, and filters, and filtrate for later use is the Rhizoma Paridis effective ingredient;
4. the filtrate of gained in getting 3., spray drying, resulting powder-tight is preserved;
5. get pulverize after Fructus Schisandrae Chinensis 0.5kg, Radix Puerariae 0.5kg, Radix Panacis Quinquefolii 0.2kg mix fine powder A, standby;
6. get Herba Orostachyos that the present invention extracts with the effective ingredient of Rhizoma Paridis and the fine powder A that makes 5. add chitosan and L-HPC, mixing adds ethanol as binding agent, the stirring granulation, treat the particle drying after, granulate is filled in No. 1 capsule shells promptly.
Embodiment 8
The present embodiment medicine is made up of according to part by weight following raw material: 4 parts of Herba Orostachyos, 1 part of Rhizoma Paridis, 0.2 part of Fructus Schisandrae Chinensis; 0.3 part of Radix Puerariae; 0.1 part of Radix Panacis Quinquefolii.
Preparation method is with embodiment 7.
Embodiment 9
The present embodiment medicine is made up of according to part by weight following raw material: 3.5 parts of Herba Orostachyos, 0.5 part of Rhizoma Paridis, 0.1 part of the Radix Astragali; 0.3 part of Radix Puerariae; Rhizoma Dioscoreae 0.1; Rhizoma Atractylodis 0.05.
Its preparation method by: the embodiment 7 described preparation method steps thing of being got it filled in is 5. changed into the Radix Astragali, Radix Puerariae, Rhizoma Dioscoreae, Rhizoma Atractylodis and gets final product, and other is with embodiment 7.
Embodiment 10
The present embodiment medicine is made up of according to part by weight following raw material: 1 part of Herba Orostachyos, 1.5 parts of Rhizoma Paridis, 0.5 part of the Radix Astragali; 0.5 part of Radix Puerariae; Rhizoma Dioscoreae 0.4; Rhizoma Atractylodis 0.25.
Its preparation method by: the embodiment 7 described preparation method steps thing of being got it filled in is 5. changed into the Radix Astragali, Radix Puerariae, Rhizoma Dioscoreae, Rhizoma Atractylodis and gets final product, and other is with embodiment 7.
Embodiment 11
The present embodiment medicine is made up of according to part by weight following raw material: 5 parts of Herba Orostachyos, 1.5 parts of Rhizoma Paridis, 6 parts of the Rhizoma Anemarrhenaes; 3 parts of Rhizoma Atractylodis.
Its preparation method by: embodiment 7 described preparation method steps are got it filled in 5., and thing changes the Rhizoma Anemarrhenae into, Rhizoma Atractylodis get final product, and other is with embodiment 7.
Embodiment 12
The present embodiment medicine is made up of according to part by weight following raw material: 1 part of Herba Orostachyos, 3 parts of Rhizoma Paridis, 8 parts of the Rhizoma Anemarrhenaes; 4 parts of Rhizoma Atractylodis.
Its preparation method by: embodiment 7 described preparation method steps are got it filled in 5., and thing changes the Rhizoma Anemarrhenae into, Rhizoma Atractylodis get final product, and other is with embodiment 7.
Embodiment 13
The present embodiment medicine is made up of according to part by weight following raw material: 5 parts of Herba Orostachyos, 1 part of Rhizoma Paridis, 1 part of Rhizoma Coptidis; 1 part of the Radix Astragali; 2 parts of Spica Prunellaes; 1 part of Rhizoma Dioscoreae; 0.5 part of Rhizoma Zingiberis; 0.5 part of Radix Paeoniae.
Its preparation method by: the embodiment 7 described preparation method steps thing of being got it filled in is 5. changed into Rhizoma Coptidis, the Radix Astragali, Spica Prunellae, Rhizoma Dioscoreae, Rhizoma Zingiberis, Radix Paeoniae and gets final product, and other is with embodiment 7.
Embodiment 14
The present embodiment medicine is made up of according to part by weight following raw material: 1 part of Herba Orostachyos, 3 parts of Rhizoma Paridis, 2 parts of Rhizoma Coptidis; 5 parts of the Radixs Astragali; 2 parts of Spica Prunellaes; 2 parts of Rhizoma Dioscoreaes; 1 part of Rhizoma Zingiberis; 1 part of Radix Paeoniae.
Its preparation method by: the embodiment 7 described preparation method steps thing of being got it filled in is 5. changed into Rhizoma Coptidis, the Radix Astragali, Spica Prunellae, Rhizoma Dioscoreae, Rhizoma Zingiberis, Radix Paeoniae and gets final product, and other is with embodiment 7.
Embodiment 15
The present embodiment medicine is made up of according to part by weight following raw material: 5 parts of Herba Orostachyos, 1 part of Rhizoma Paridis, 6 parts of Rhizoma Coptidis; 1 part of Radix Et Rhizoma Rhei, 1 part in Radix Glycyrrhizae: 5 parts in Poria, 5 parts of the Rhizoma Atractylodis Macrocephalaes, 9 parts of Rhizoma Polygonatis.
Its preparation method by: the embodiment 7 described preparation method steps thing of being got it filled in is 5. changed into Rhizoma Coptidis, Radix Et Rhizoma Rhei, Radix Glycyrrhizae, Poria, the Rhizoma Atractylodis Macrocephalae, Rhizoma Polygonati and gets final product, and other is with embodiment 7.
Embodiment 16
The present embodiment medicine is made up of according to part by weight following raw material: 2 parts of Herba Orostachyos, 5 parts of Rhizoma Paridis, 9 parts of Rhizoma Coptidis; 3 parts of Radix Et Rhizoma Rhei, 3 parts in Radix Glycyrrhizae: 8 parts in Poria, 8 parts of the Rhizoma Atractylodis Macrocephalaes, 10 parts of Rhizoma Polygonatis.
Its preparation method by: the embodiment 7 described preparation method steps thing of being got it filled in is 5. changed into Rhizoma Coptidis, Radix Et Rhizoma Rhei, Radix Glycyrrhizae, Poria, the Rhizoma Atractylodis Macrocephalae, Rhizoma Polygonati and gets final product, and other is with embodiment 7.
Claims (10)
1, a kind of compound Chinese medicinal preparation for the treatment of type ii diabetes, blood sugar lowering is characterized in that composition comprises Herba Orostachyos and Rhizoma Paridis in the preparation.
2, the compound Chinese medicinal preparation of treatment type ii diabetes according to claim 1, blood sugar lowering is characterized in that main component is the mixture of Herba Orostachyos and Rhizoma Paridis in the preparation, is by weight proportion: Herba Orostachyos 1-3.5 part, Rhizoma Paridis 0.5-1.5 part.
3, the compound Chinese medicinal preparation of treatment type ii diabetes according to claim 2, blood sugar lowering, the part by weight that it is characterized in that Herba Orostachyos and Rhizoma Paridis are 2.5 parts of Herba Orostachyos, 1 part of Rhizoma Paridis.
4, the compound Chinese medicinal preparation of treatment type ii diabetes according to claim 1, blood sugar lowering is characterized in that being made up of according to part by weight following raw material: Herba Orostachyos 1-4 part, Rhizoma Paridis 1-3 part, Fructus Schisandrae Chinensis 0.2-0.5 part; Radix Puerariae 0.3-0.5 part; Radix Panacis Quinquefolii 0.1-0.2 part.
5, the compound Chinese medicinal preparation of treatment I type i diabetes according to claim 1, blood sugar lowering is characterized in that being made up of according to part by weight following raw material: Herba Orostachyos 3.5-1 part, Rhizoma Paridis 0.5-1.5 part, Radix Astragali 0.1-0.5 part; Radix Puerariae 0.3-0.5 part; Rhizoma Dioscoreae 0.1-0.4; Rhizoma Atractylodis 0.05-0.25.
6, the compound Chinese medicinal preparation of treatment type ii diabetes according to claim 1, blood sugar lowering is characterized in that being made up of according to part by weight following raw material: Herba Orostachyos 5-1 part, Rhizoma Paridis 1.5-3 part, Rhizoma Anemarrhenae 6-8 part; Rhizoma Atractylodis 3-4 part.
7, the compound Chinese medicinal preparation of treatment I type i diabetes according to claim 1, blood sugar lowering is characterized in that being made up of according to part by weight following raw material: Herba Orostachyos 5-1 part, Rhizoma Paridis 1-3 part, Rhizoma Coptidis 1-2 part; Radix Astragali 1-5 part; 2 parts of Spica Prunellaes; Rhizoma Dioscoreae 1-2 part; Rhizoma Zingiberis 0.5-1 part; Radix Paeoniae 0.5-1 part.
8, the compound Chinese medicinal preparation of treatment type ii diabetes according to claim 1, blood sugar lowering is characterized in that being made up of according to part by weight following raw material: Herba Orostachyos 5-2 part, Rhizoma Paridis 1-5 part, Rhizoma Coptidis 6-9 part; Radix Et Rhizoma Rhei 1-3 part, Radix Glycyrrhizae 1-3 part: Poria 5-8 part, Rhizoma Atractylodis Macrocephalae 5-8 part, Rhizoma Polygonati 9-10 part.
9, a kind of preparation method for the treatment of the compound Chinese medicinal preparation of type ii diabetes, blood sugar lowering is characterized in that it comprises following processing step:
1. get the dry herb of Herba Orostachyos, coarse crushing adds alcohol reflux, filters, and medicinal residues wash with small amount of ethanol, merge extractive liquid, and cleaning mixture, and decompression recycling ethanol filters filtrate for later use to not having the alcohol flavor and concentrating;
2. the filtrate of gained in getting 1., spray drying, the powder-tight of gained is preserved;
3. get the dry medical material of Rhizoma Paridis, be ground into particulate, add the water reflux, extract,, filter, medicinal residues wash with low amounts of water, and merge extractive liquid, and cleaning mixture concentrate, and standing over night filters filtrate for later use;
4. the filtrate of gained in getting 3., spray drying, resulting powder-tight is preserved;
5. 3.5-1: the 0.5-1.5 effective ingredient of getting the orostachys fimbriatus effective component of said extracted and Rhizoma Paridis adds suitable pharmaceutic adjuvant and is prepared into Chinese traditional compound medicine of the present invention by weight proportion.
10, claim 1 or the 2 described medicines application in preparation treatment diabetes medicament.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100477929A CN100355440C (en) | 2005-11-23 | 2005-11-23 | Compound Chinese medicinal preparation for treating type II diabetes and lowering blood sugar and its preparation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100477929A CN100355440C (en) | 2005-11-23 | 2005-11-23 | Compound Chinese medicinal preparation for treating type II diabetes and lowering blood sugar and its preparation method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1785348A true CN1785348A (en) | 2006-06-14 |
| CN100355440C CN100355440C (en) | 2007-12-19 |
Family
ID=36783016
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2005100477929A Expired - Fee Related CN100355440C (en) | 2005-11-23 | 2005-11-23 | Compound Chinese medicinal preparation for treating type II diabetes and lowering blood sugar and its preparation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100355440C (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102641362A (en) * | 2012-04-19 | 2012-08-22 | 陈正梅 | Medicine for treating II type diabetes mellitus |
| CN115089678A (en) * | 2022-08-10 | 2022-09-23 | 王玉刚 | A Chinese medicinal composition for treating diabetes, and its preparation method |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1257724C (en) * | 2003-10-20 | 2006-05-31 | 中国农业科学院蜜蜂研究所 | Composition for preventing and treating diabets mellitus |
-
2005
- 2005-11-23 CN CNB2005100477929A patent/CN100355440C/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102641362A (en) * | 2012-04-19 | 2012-08-22 | 陈正梅 | Medicine for treating II type diabetes mellitus |
| CN115089678A (en) * | 2022-08-10 | 2022-09-23 | 王玉刚 | A Chinese medicinal composition for treating diabetes, and its preparation method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100355440C (en) | 2007-12-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1243559C (en) | Herbal composition and medicament against diabetes mellitus type II manufactured thereof | |
| CN1840166A (en) | Modern Chinese medicinal oral liquid of 'Wen Dan Tang' and preparation method thereof | |
| CN1899501A (en) | Chinese medicine compound preparation for treating laryngopharyngitis and tonsillitis and its preparing method | |
| CN1557842A (en) | Process for extracting polygonatum polysaccharides, preparing process for medical preparation and use thereof | |
| CN1220518C (en) | Chinese medicinal composition for anti-cancer and analgesia, its preparation method and its application in the preparation of product and medicine for treating cancer and analgesia | |
| CN1200723C (en) | White peony and licorice extract and its prepn process | |
| CN101028320A (en) | Vegetable medicinal composition with hypoglycemic and hypolipidemic functions and its making method | |
| CN1899536A (en) | Medicine for treating diabetes and hephrosis and its preparing method | |
| CN1785348A (en) | Compound Chinese medicinal preparation for treating type II diabetes and lowering blood sugar and its preparation method | |
| CN100344315C (en) | Medicinal composition for promoting bone fracture healing and its preparing method | |
| CN1562337A (en) | Chinese traditional medicine for treating insufficiency of kidney-yang and preparaton method | |
| CN1511583A (en) | Chinese rose extract and its preparing method and use | |
| CN100337665C (en) | Oral medicine for treating diabetes | |
| CN1857588A (en) | Quality control method for Xianlinggubao preparation | |
| CN1733089A (en) | Medicine for treating diabetes and its complications and process for preparing the same | |
| CN1194743C (en) | Chinese medicinal composition for treating atrophic arthritis, preparing method and quality controlling method thereof | |
| CN1634463A (en) | Medicine for treating diabetes and production method thereof | |
| CN1569195A (en) | Rhodiola sacra soft capsule and its preparation | |
| CN1903279A (en) | Method for extraction and separation of Immature Bitter orange | |
| CN1742936A (en) | Natural medicine preparation for treating diabets and preparing method | |
| CN1872232A (en) | Composition of medication for curing cerebrovascular disease, and preparation method | |
| CN1775277A (en) | Natural medicine composition for preventing and treating metabolic syndrome | |
| CN1569160A (en) | Chinese medicinal preparation for treating cardiovascular and cerebrovascular diseases and its preparing process | |
| CN1682934A (en) | Yuquan extractum and its medicine composition and use | |
| CN1253145C (en) | Blood sugar reducing skin-care cream specially for curing diabetes and its producing method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: SHENYANG JINCHANG PHARMACEUTICAL CO., LTD. Free format text: FORMER OWNER: LIAONING MEDICINE GROUP CO., LTD. Effective date: 20110610 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 110005 NO. 33, SOUTH LIUMA ROAD, HEPING DISTRICT, SHENYANG CITY, LIAONING PROVINCE TO: 110179 NO. 99, GAOKE ROAD, HUNNAN NEW DISTRICT, SHENYANG CITY, LIAONING PROVINCE |
|
| TR01 | Transfer of patent right |
Effective date of registration: 20110610 Address after: Hi Tech road Shenyang city Liaoning province 110179 Hunnan New District No. 99 Patentee after: Shenyang Jinchang Pharmaceutical Co., Ltd. Address before: 110005, No. six, South 33 Road, Heping District, Liaoning, Shenyang Patentee before: Liaoning Medicine Group Co., Ltd. |
|
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20071219 Termination date: 20121123 |