CN1772388A - Multichannel array micro liquid transferrer - Google Patents
Multichannel array micro liquid transferrer Download PDFInfo
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- CN1772388A CN1772388A CN 200410094575 CN200410094575A CN1772388A CN 1772388 A CN1772388 A CN 1772388A CN 200410094575 CN200410094575 CN 200410094575 CN 200410094575 A CN200410094575 A CN 200410094575A CN 1772388 A CN1772388 A CN 1772388A
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Abstract
The multichannel array micro liquid transferrer is for mass metering, transferring and distribution of micro volume liquid. It has base board, channel on the base board, sampling needle in the bottom of valve channel and with liquid metering channel communicated with the valve channel. The liquid metering channel has cross section area of several square microns to several square millimeters, greater than that of the intersection between the liquid metering channel and the valve channel. It may be used as the multichannel micro liquid transferring pipet, multichannel micro sampler, 2D array sample applicator, etc in lab. Owing to the pressure for solution to enter the valve channel greater than that to enter the liquid metering channel, the valve channel acts as valve. Canceling negative pressure or applying positive pressure can outflow the solution from the liquid metering channel to complete the solution metering, transferring and distribution in the volume level of microliter or even picoliter.
Description
Technical field
The present invention relates to a kind of transfer device of measuring of small volume of liquid, especially a kind ofly can carry out the multichannel array micro liquid transferrer that multichannel micro liquid is measured and shifted simultaneously.
Background technology
The importance of life science and environmental science is growing, and the analysis of the screening of medical diagnosis, novel drugs and a large amount of samples all needs parallel big flux to shift the technology of minimum volume (microlitre, receive liter, skin liter) liquid to save sample consumption and protection environment.
The conventional multichannel pipette (multi channel pipette) that uses can only reach micro updating and cost an arm and a leg.More small size (skin rise to receive liter) solution is measured the device and the technology of transfer at present, mainly can be divided into two big classes: first kind method is ink jet type (Inkjet), mainly take near jet apertures, to install piezo-electric crystal (as the Nano-Plotter technology of German GeSiM company, point sample technology with U.S. Packard company), or use syringe pump to spray small volume solution in conjunction with the method for high-speed solenoid valve (as U.S. CartesianTechnologies company), B.J.Larson (the B.J.Larson of nearest U.S. Wisconsin-Madison university, Reviews of Scientific Instruments, 2004, Vol.75, No.4 832-836) uses ultrasonic wave to drive piezo-electric crystal.Second class methods are that the pin contact shifts (pin transfer), and its cardinal principle is knocked at the surface of solids after getting solution for using the rigidity fine needle, dipping in, and the liquid of micro-volume is transferred on the solid.The frame for movement of this two classes technology is more complicated all, can't array, shift when also can only carry out the dozens of sample simultaneously at the most.
In addition, Monica M.Palcic (the J.Lorieau of Canada Alberta university, G.K.Shoemaker and M.M.Palcic, Anal.Chem., 2003,75,6351-6354) before syringe needle, connect a capillary, utilize the transmission of gas pressure decrement to receive upgrading the measuring of solution, and it is applied to the Capillary Electrophoresis sample introduction, this method can not array.Germany Freiburg Roland Zengerle (the Roland Zengerle of university, Deutsche Bundespatent, 19,913,076,1999, the same year PCT) the TopSpot technology of invention and the array sampling head technology of the Tseng Fangang of Taiwan National Tsing Hua University invention, can carry out the micro solution of array and distribute, but it just distributes the solution of appended liquid storing part several times, can't carry out the transfer of measuring of solution.Minoru Seki (the Masumi Yamada and MinoruSeki of Japan Osaka Prefecture university, Anal.Chem., 2004,76,895-899) and AniruddhaPuntambekar (the Aniruddha Puntambekar of U.S. Cincinnati university, et al., Lab on a chip, 2002,2,213-128) integrated a plurality of aggressive valve on the PDMS micro-fluidic chip be used for the solution to microchannel injection dispense nanoliter level, but this type of technology can't be carried out measuring and shifting of solution.
In sum, still do not have at present and can array (one dimension or two dimension) measure the technology that shifts small volume (the microlitre order of magnitude to skin rises the order of magnitude).
Summary of the invention
The present invention aims to provide a kind of multichannel array micro liquid transferrer that can measure, shift and distribute small volume (the microlitre order of magnitude to skin rises the order of magnitude) liquid in enormous quantities.Can be used as laboratory multichannel micropipette, multichannel micro-sampling device, two-dimensional array spot sample device or the like.
The present invention is provided with substrate, substrate is provided with the passage of at least one up/down perforation, claim valve passage, locate to establish sampling probe at base plate bottom corresponding to valve passage bottom (claiming valve passage import or inlet), the amount liquid passage of sampling probe communicates with valve passage, the cross-sectional area of amount liquid passage is greater than the cross-sectional area of amount liquid passage and valve passage intersection, for counting square micron extremely between several square millimeters.
The surface of amount liquid passage and valve passage is lyophoby surface (as to measure solution be the aqueous solution, then is hydrophobic surface, otherwise, then be water-wetted surface).Valve passage can be cylindricality, or bell, or taper, or cydariform, or spherical.Amount liquid passage can be cylindricality, or bell, or taper, or cydariform, or spherical, and its volume rises between several microlitres for the number skin.
Its character of surperficial lyophoby of said amount liquid passage and valve passage is the character of the material of the character of bulk material or finishing or coating.The spacing of channel unit is between several microns to several millimeters.The length of sampling probe is between several microns to several centimetres.The diameter of sampling probe is between several microns to several centimetres.
The similar integrated package of the present invention, be integrated with the channel unit that several connects to thousands of roads, channel unit is made up of the amount liquid passage and the valve passage that communicate, the sectional area of both intersections is less than the sectional area of amount liquid passage, the surface of amount liquid passage and valve passage is the lyophoby surface, and (as to measure solution be the aqueous solution, then be hydrophobic surface, otherwise, then be water-wetted surface).When the outlet side at valve passage applied negative pressure, solution was inlet liquid passage.Because solution enters valve passage need be than the much bigger pressure of inlet liquid passage, therefore regulate suitable pressure, can make the solution amount of being full of liquid passage and do not enter valve passage, promptly valve passage plays the valve effect.Remove negative pressure or apply malleation, the solution in then can the amount of making liquid passage flows out, thereby finishes measuring of solution, shifts and distributes.This shows that the advantage that the present invention has is: move that liquid is long-pending can to reach microlitre to the skin liter, parallel flux greatly (for example can tens of roads one row, thousands of road two-dimensional array), simple in structure and price is very cheap, washing easily, reusable, also can disposablely use, reduce cross pollution.Can be used as laboratory multichannel micropipette, multichannel micro-sampling device, the two-dimensional array spot sample device, or the like.
Description of drawings
Fig. 1 is the structural representation of the embodiment of the invention.
Fig. 2 is the component units structural representation of Fig. 1.
The specific embodiment
Following examples will be in conjunction with the accompanying drawings, introduces particular content of the present invention in detail.
As shown in Figure 1, the present invention is provided with substrate 3, substrate 3 is provided with number to thousands of channel units, the sampling probe 2 of hollow is located to establish in valve passage 1 bottom (claiming the valve passage inlet) at up/down perforation, the amount liquid passage 4 of sampling probe 2 communicates with valve passage 1, the cross-sectional area of amount liquid passage is greater than the cross-sectional area of amount liquid passage 4 with valve passage 1 intersection, for counting square micron extremely between several square millimeters.For example, the cross-sectional area of amount liquid passage is at least 5 times of the cross-sectional area of amount liquid passage 4 and valve passage 1 intersection.Valve passage and amount liquid passage are cylindricality, and the surface of amount liquid passage and valve passage is lyophoby surface (as to measure solution be the aqueous solution, then is hydrophobic surface, otherwise, then be water-wetted surface).The volume of amount liquid passage is that skin rises between the microlitre.
Its character of surperficial lyophoby of said amount liquid passage and valve passage is the character of the material of the character of bulk material or finishing or coating.The spacing of each channel unit is between several microns to several centimetres, also can directly sampling probe is inserted among the Xiao Chi of plate, carry out batch sampling/sample introduction according to the pond of ELISA Plate or PCR plate apart from setting.The length of sampling probe is between several microns to several centimetres.The diameter of sampling probe is between several microns to several centimetres.
In Fig. 2, amount liquid passage 4 and valve passage 1 communicate.The sectional area of both intersections is less than the sectional area of amount liquid passage, and for example the sectional area of valve passage is tens of microns, and the sectional area of amount liquid passage is hundreds of microns.Amount liquid passage and outer wall form the sampling probe of hollow.Outlet side at valve passage applies negative pressure, makes solution inlet liquid passage.Because solution enters valve passage need be than the big pressure of inlet liquid passage, therefore regulate suitable pressure, can make the solution amount of being full of liquid passage and do not enter valve passage, promptly valve passage plays the valve effect.When removing negative pressure or applying malleation, the solution in then can the amount of making liquid passage flows out, thereby finishes measuring, shift and distributing of solution.The volume of measuring solution can be by the volume settings of amount liquid passage.As to measure the liquid passage be not that cylindricality but bell or taper then can be regulated by pressure, and the height of control solution inlet liquid passage is measured the solution of different volumes.
Claims (9)
1, multichannel array micro liquid transferrer, it is characterized in that being provided with substrate, substrate is provided with the passage of at least one up/down perforation, claim valve passage, locate to establish sampling probe at base plate bottom corresponding to valve passage bottom (claiming valve passage import or inlet), the amount liquid passage of sampling probe communicates with valve passage, and the cross-sectional area of amount liquid passage is greater than the cross-sectional area of amount liquid passage and valve passage intersection, for counting square micron extremely between several square millimeters.
2, multichannel array micro liquid transferrer as claimed in claim 1 is characterized in that the surface of said amount liquid passage and valve passage is the lyophoby surface.
3, multichannel array micro liquid transferrer as claimed in claim 1 is characterized in that valve passage is a cylindricality, or bell, or taper, or cydariform, or spherical.
4, array channel pipettor as claimed in claim 1, the amount of it is characterized in that liquid passage is a cylindricality, or bell, or taper, or cydariform, or spherical.
5, as claim 1 or 4 described multichannel array micro liquid transferrers, its volume of the amount of it is characterized in that liquid passage is that skin rises between the microlitre.
6, multichannel array micro liquid transferrer as claimed in claim 1 or 2, its character of surperficial lyophoby that it is characterized in that said amount liquid passage and valve passage are the character of the material of the character of bulk material or finishing or coating.
7, multichannel array micro liquid transferrer as claimed in claim 1, the spacing that it is characterized in that channel unit is between several microns to several centimetres.
8, multichannel array micro liquid transferrer as claimed in claim 1, the length that it is characterized in that sampling probe is between several microns to several centimetres.
9, as claim 1 or 8 described multichannel array micro liquid transferrers, the diameter that it is characterized in that sampling probe is between several microns to several millimeters.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100945750A CN100348324C (en) | 2004-11-10 | 2004-11-10 | Multichannel array micro liquid transferrer |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100945750A CN100348324C (en) | 2004-11-10 | 2004-11-10 | Multichannel array micro liquid transferrer |
Publications (2)
| Publication Number | Publication Date |
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| CN1772388A true CN1772388A (en) | 2006-05-17 |
| CN100348324C CN100348324C (en) | 2007-11-14 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNB2004100945750A Expired - Fee Related CN100348324C (en) | 2004-11-10 | 2004-11-10 | Multichannel array micro liquid transferrer |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102847567A (en) * | 2012-08-24 | 2013-01-02 | 浙江硕华医用塑料有限公司 | Microscale sampling rod |
| CN103140283A (en) * | 2010-10-29 | 2013-06-05 | 国际商业机器公司 | Multilayer microfluidic probe head with immersion channels and fabrication thereof |
| CN107754963A (en) * | 2017-10-31 | 2018-03-06 | 南京航空航天大学 | Ultrasonic pipettor |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6713309B1 (en) * | 1999-07-30 | 2004-03-30 | Large Scale Proteomics Corporation | Microarrays and their manufacture |
| CN1132940C (en) * | 1999-12-16 | 2003-12-31 | 吴昌 | Method for producing biological chip |
| CN1195984C (en) * | 2002-07-12 | 2005-04-06 | 厦门大学 | Surface tension driving liquid flow chiplized high-density micro-array liquid transferring equipment |
| JP3865134B2 (en) * | 2003-01-09 | 2007-01-10 | 横河電機株式会社 | Biochip cartridge |
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2004
- 2004-11-10 CN CNB2004100945750A patent/CN100348324C/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103140283A (en) * | 2010-10-29 | 2013-06-05 | 国际商业机器公司 | Multilayer microfluidic probe head with immersion channels and fabrication thereof |
| CN103140283B (en) * | 2010-10-29 | 2015-10-07 | 国际商业机器公司 | There is the multilayer miniflow detecting head and making thereof that immerse passage |
| US9745949B2 (en) | 2010-10-29 | 2017-08-29 | International Business Machines Corporation | Multilayer microfluidic probe head with immersion channels and fabrication thereof |
| CN102847567A (en) * | 2012-08-24 | 2013-01-02 | 浙江硕华医用塑料有限公司 | Microscale sampling rod |
| CN107754963A (en) * | 2017-10-31 | 2018-03-06 | 南京航空航天大学 | Ultrasonic pipettor |
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| Publication number | Publication date |
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| CN100348324C (en) | 2007-11-14 |
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