CN1132940C

CN1132940C - Method for producing biological chip

Info

Publication number: CN1132940C
Application number: CN 99120439
Authority: CN
Inventors: 吴昌; 吴明
Original assignee: Individual
Current assignee: Individual
Priority date: 1999-12-16
Filing date: 1999-12-16
Publication date: 2003-12-31
Anticipated expiration: 2019-12-16
Also published as: CN1280195A

Abstract

The present invention relates to a method for producing a biological chip, which comprises the following steps: filling biological samples in the distributor of an arranging machine, controlling a mechanical hand by a computer, and arranging liquid samples in the distributor on a chip medium by a distributing head. The present invention is characterized in that the distributor is filled with store liquid and is provided with a guide board, a distributing pipe filled with the samples is inserted into the hole of the guide board to make the guide board lowered, and the distributing head of the distributing pipe is in contact with the chip. The present invention has the advantages of large sample storing quantity and long sample storing period. Each distributing pipe can hold the sample once, the steps of cleaning, drying and filling the sample can be canceled, the distributing pipes can be combined in high density, and the chip can be produced quickly. The present invention is suitable for commercial mass production.

Description

A kind of method of making biochip

The present invention relates to a kind of method of biochip and used device made.

At present, when making biochip, generally all use the arrangement machine, it is mainly by a worktable, can be made up of along mechanical manipulator and a divider that three-dimensional moves computer control.When making biochip,, the sample of being got is discharged on the chip medium, varies behind the cleaning divider, with same divider fresh sample is discharged on the same chip medium again, repeat these actions and can produce biochip with the divider sampling.Existing divider has dispense needles, thereby be also referred to as the pin type divider, during use, be stained with sample with the pin type divider, then sample spot on chip medium, it can only discharge sample tens times, each dispense needles is used for a plurality of samples in the pin type divider, when varying, needs to clean, dry up, fill multiple operation stepss such as sample, and owing to vary, the combined density of dispense needles on the pin type divider is little (＜10/cm ²), film-making speed is slow, only is applicable to the making of the biochip of indefinite form on a small scale.

The object of the present invention is to provide a kind of method of making biochip, it can overcome the above-mentioned deficiency of prior art.

A kind of method of making biochip, comprise divider can biological sample with the arrangement machine, with computer controlling machine tool hand, liquid sample in the divider is emitted on the chip medium through distributor head, it is characterized in that described divider is equipped with holds liquid, and disposes a guide plate, and the distribution piping that pours into sample is inserted in the hole of guide plate, allow guide plate descend, the distributor head of distribution piping is contacted with chip medium.

Implement to make the used device of method of biochip, comprise one by the worktable of computer control and the mechanical manipulator and the divider that can move along three-dimensional arranged, it is characterized in that this divider has distribution piping, distribution piping has distributor head, this distributor head by conduit with hold liquid and be connected, and dispose a guide plate.

Advantage of the present invention is that the amount of storage of sample is big, and the sample staging life is long.A distribution piping is only adorned sample once in the film-making process, save cleaning, drying, fill steps such as sample, so distribution piping can make up to high-density, makes that film-making speed is fast, is suitable for shiploads of merchandise production.

Below by embodiment and description of drawings the present invention.

Accompanying drawing is the structural representation of divider of the present invention.

The used device of the present invention is an arrangement machine, comprise one by the worktable of computer control and mechanical manipulator that can move along three-dimensional and the divider that comprises a plurality of distribution pipings are arranged, it is characterized in that this divider has distributor head (1), this distributor head (1) by conduit (3) with hold liquid (2) and be connected, and dispose a guide plate (4).Distributor head (1) is interchangeable, and it is contained in the lower end of conduit (3), and the upper end of conduit (3) is equipped with and is held liquid (2), distributor head (1), holds liquid (2) and conduit (3) and constitutes a distribution piping.The cross section that wherein holds liquid (2) is circular or oval, rectangle, and the long 1-100mm of body, the girth of its cross section are 0.5-50mm.Conduit (3) is a tubular, external diameter 0.1-5mm, and length is 1-100mm, and the external diameter of distributor head (1) is 0.1-5mm, and length is 0.1-3mm, and the shape of the terminal liquid outlet of distributor head (1) is flat, and is circular arc or pointed.The volume of distribution piping is 0.1 μ l-10ml.The described liquid (2) that holds has lid, can prevent the contamination by dust sample.The plate face area of guide plate (4) is 5mm ²-100cm ², a plurality of holes are arranged above, a guide plate (4) has one deck, two-layer or three ply board face, and the position in the hole on the multi-ply wood face is corresponding up and down, and a distribution piping can be inserted in each group hole, has its intermediate plate of guide plate (4) of three ply board face can lateral adjustments.Described distributor head (1) but in filling sponge, can limit the speed and the flow of sample.These distribution pipings can move with guide plate (4) under the drive of mechanical manipulator.

2,000 kinds of different gene clone samples are arranged in a certain gene pool, after these 2,000 gene samples are amplified, purified, be filled into respectively in 2,000 distribution pipings of arrangement machine, each distribution piping all has identification marking.Each distribution piping has a distributor head (1), and it is contained in the lower end of conduit (3), and the upper end of conduit (3) is equipped with and is held liquid (2).These 2,000 distribution pipings are divided into 5 groups, and every group has 400 distribution pipings, and according to the present invention, a component pipe arrangement and a guide plate (4) are used in combination, and constitute 5 dividers altogether.This guide plate (4) is made up of two-layer porous plate, and the position in hole is corresponding, concentric on each laminate, and a distribution piping can be inserted in each group hole, and the latter can be up and down.5 dividers all by on the worktable that is placed on the arrangement machine, are placed with 100 chip medium simultaneously on worktable.Get the 1st divider with the computer manipulator robot, allow be fixed on and move by the guide plate (4) on the mechanical manipulator of computer control, when guide plate (4) when dropping to certain position, distributor head (1) just contacts with chip medium, and liquid sample is emitted on the chip medium, when guide plate (4) when continuing to move down, the distribution piping that contacts with chip medium can not continue to move down yet, till contacting the medium face.Under the drive of mechanical manipulator, when guide plate moved up, distribution piping also moved on together.When divider was lifted to certain altitude again, all distribution pipings were subjected to the downward contention effect of external force and are returned to starting position again.Like this, just 400 kinds of samples have been emitted on each chip medium, one has 100 chip medium.And then repeat this process, the sample in the 2nd divider is emitted on these 100 chip medium, like this, through 5 same operations, just made 100 each have the gene chip of 2000 sample spot.The required time is 5 minutes.After changing new dieelctric sheet, available original distribution piping is produced another batch chip.

Claims

1. method of making biochip, comprise divider can biological sample with the arrangement machine, with computer controlling machine tool hand, liquid sample in the divider is emitted on the chip medium through distributor head (1), it is characterized in that described divider is equipped with holds liquid (2), and disposes a guide plate (4), and the distribution piping that pours into sample is inserted in the hole of guide plate (4), allow guide plate (4) descend, the distributor head (1) of distribution piping is contacted with chip medium.