CN1772288A - Recipe of skin repairing composition - Google Patents

Abstract

The recipe of skin repairing composition includes: EDTA-2Na, glycerin, 1, 3-butanediol, metallothionein zinc, heparin sodium, recombinant human epidermal growth factor, liposoluble preservative, recombinant human stem cell factor, and buffering solution. The skin repairing composition in the recipe can speed proliferation of stem cell to split and convert into great amount of transient proliferating cells. The addition of the epidermal growth factor can induce the fast proliferation of the transient proliferating cells and improve intercellular substance circumstance to regulate the growth of skin cell. The metallothionein can eliminate free radical and other cell metabolism products during cell growth specifically, so as to create clean circumstance for sustained effective action of stem cell factor and epidermal growth factor on the cells.

Description

A kind of Recipe of skin repairing composition

Technical field:

The present invention relates to a kind of Recipe of skin repairing composition, relate to the medical cosmetology product scope.

Technical background:

Skin is the organ of human body maximum, and epidermis is positioned at the outermost layer of skin, be can self renewal tissue (upgrading 1 time every month approximately).(keratinocyte stem cell KSC) as the tissue specificity stem cell, has powerful multiplication capacity to the epidermal stem cells of basal layer, is the origin of epidermis various types of cells.In recent years, because the research of aspects such as tissue engineering skin, skin genetic diseases and genetic treatment of tumor, KSC receives publicity day by day, simultaneously, becomes the focus and the focus of research aspect medical cosmetology.The propagation of KSC is relevant with following factor with differentiation: (one) stem cell factor (SCF).RhMGF (recommbintant human stemcell, rhSCF) be that the C-KIT site combines having of producing of human body self cell on the stem cell that is in specificity, high efficiency, specificity and the human body under " dormancy " state, thereby activate stem cell and enter division growth, the metabolism of Skin Cell is speeded, change the skin aging structure rapidly, make skin present rejuvenation.Clinical experiment confirms: after SCF was injected into human body, a large amount of stem cell that exist had specific stimulation in the bone marrow, can induce at short notice to produce a large amount of complete blood cell.The research group experiment confirm of U.S. Boston university and Dermatology system of Columbia university, SCF has the effect of efficiently stimulating proliferation to the epidermal stem cells (KSC) of skin basal layer, has convincingly demonstrated the application prospect of stem cell factor (SCF) in skin repair.(2) epidermal growth factor (EGF).Over 40 years, people have had sufficient understanding to the mechanism of action and the actual effect of EGF from Cohn table of discovery skin growth factor (EGF).EGF has two effect characteristics in skin: 1, sphere of action is extensive, comprises epidermis cell, Interstitial cell, fibroblast, vascular endothelial cell and smooth muscle cell.2, EGF can directly or indirectly regulate extracellular matrix (ECM) by cell, influences molecular composition, physics and chemistry state and the metabolic function of ECM.ECM is the microenvironment of cells survival, is also undertaking the cell signalling function, is directly connected to cell proliferation, growth and survival condition.EGF uses in a large number at medicine and cosmetic field at present, under the effect of EGF, quickens the renewal of epidermis greatly, makes the water content of epidermal tissue also become higher, makes skin become delicate.The epidermis cell rejuvenation, chromatoplast is corresponding less in the endochylema, and the dead cell residue reduces in the epidermis, and it is fair-skinned flawless in vain that cutaneous manifestations is gone out, and EGF has the physiology class's effect of dispelling, and widely applies to confirm that its effect is also very clear and definite.(3) metallothionein (MT).Nineteen fifty-seven Margoshes and Vallee isolate a kind of new protein from the animal organ when research metal biological action, it contains abundant sulfydryl, can chela and a large amount of heavy metal ion.Present scientific research has confirmed metallothionein, and (Metallothionein MT) has three big functions: 1, remove free radical.It removes free radical scavenger one superoxide dismutase (SOD) Qiang Yiwan full-time in the energy force rate body of free radical doubly.2, biologic detoxication function can be in conjunction with heavy metal of body (as lead, arsenic, hydrargyrum etc.), the zinc element that lacks in the while added body.3, radiation-resisting functional can be effectively to ionizing radiation-resistant and electromagnetic radiation, is one of effective substances of finding at present.The applied research of metallothionein (MT) aspect medicine, food, health product and cosmetics is relatively more abundant, and existing a large amount of application.

The present invention utilizes up-to-date biotechnology, thinks that through a large amount of theoretic discussion and laboratory researches the reconstruction of skin repair and epidermis cell is a very complex process, and the growth inducing factor mechanism of Skin Cell also is complicated.The a certain factor of single use, after using a certain amount of and time, its marginal effect rate can sharply descend and (strengthen factor use amount and increase factor action time, and the growing multiplication speed of Skin Cell and amount can in direct ratioly not increase, may can suppress cell division, growth on the contrary), because human body is regulated body cell by positive-negative feedback all the time, make it to keep a kind of dynamic balance state.From the cytobiology angle, owing to when using the growth of effect sex factor effect inducing cells, accelerated the metabolism of cell, produced a large amount of metabolite (as free radical etc.) a large amount of.The accumulation of a large amount of metabolites in cell and in the intercellular substance can have serious destruction and inhibitory action by pair cell, why Skin Cell growth and repair and can all do not finished by monofactor that Here it is.

Summary of the invention:

For solving in the medical cosmetology cosmetics, avoid Skin Cell that there is toleration in cosmetics simultaneously and cause the cost of cosmetics soaring skin nursing and cytothesis high efficiency.The present invention releases a kind of Recipe of skin repairing composition.At first, the specificity of stem cell factor (SCF), high efficiency, transitivity start the stem cell that is in " dormancy " state, promote stem cell fast breeding, division to be converted into a large amount of of short duration proliferative cells.At this moment, EGF's adds affiliation from inducing of short duration proliferative cell cell fast breeding and improving the growth that the intercellular substance environment is regulated and control Skin Cell.The products of cellular metabolism such as free radical that produce in the metallothionein "white expert" department scavenger cell growth course, its effect are just as rubbish " street cleaner ", for SCF, EGF continuous and effective act on the environment that cell is created " totally ".

Technical scheme:

This novel skin reparation combination is undertaken by following technical scheme:

(1) preparation of buffer solution (being upgraded to example) to prepare 1

1. sodium hydrogen phosphate (Na2HPO4.12H2O 0.01mol/L)-----720ml

2. potassium dihydrogen phosphate (KH2PO4 0.01mol/L)-----280ml

3. pH value is adjusted to about 7.15 after above two kinds of liquid mixing

Below used liquid be above PBS buffer system.

(2) preparation of novel skin reparation combination:

Material name content (g/kg)

EDTA-2Na 1.0

Glycerol 50.0

1,3 butanediol 50.0

Metallothionein white zine (MT) 5mg

Oil-solvent preservative 2.0

(buffer solution 849ml)

Heparin sodium 5.0

Recombinant human epidermal growth factor (rhEGF) 2.0mg

RhMGF (rhSCF) 1.0mg

The invention has the beneficial effects as follows:

A kind of Recipe of skin repairing composition for mainly containing effective constituent, is mainly reflected in following several respects from the practical clinical effect with SCF, EGF and MT:

1, SCF directly acts on stem cell, starts the differentiation and proliferation of stem cell.Keep " activation " state that is in of cell, allowed Skin Cell be in " youth " state.

2, EGF is converted into sophisticated epidermis cell in a large number by inducing of short duration proliferative cell, Skin Cell is in fast updates.

3, MT removes metabolite (free radical), for the effect of SCF, EGF provides an inside and outside environment of good cell, simultaneously, because it has powerful effect dispeling subcutaneous mottle, thereby has remedied the deficiency of SCF, EGF effect in this respect.

Claims (2)

1, a kind of Recipe of skin repairing composition is characterized in that, this Recipe of skin repairing composition is by following preparation of raw material: EDTA-2Na; Glycerol; 1,3 butanediol; Metallothionein white zine (MT); Heparin sodium; Recombinant human epidermal growth factor (rhEGF); Oil-solvent preservative; RhMGF (rhSCF); Buffer solution.2, a kind of manufacture method that contains the detoxication vinegar of tea polyphenols composition according to claim 1 is characterized in that polyphenol content is 0.1%-0.5% in traditional vinegar.

2, a kind of Recipe of skin repairing composition according to claim 1 is characterized in that this novel skin reparation combination undertaken by following technical scheme:

(1) preparation of buffer solution (being upgraded to example) to prepare 1:

Sodium hydrogen phosphate (Na2HPO4.12H2O 0.01mol/L)-----720ml

Potassium dihydrogen phosphate (KH2PO4 0.01mol/L)-------280ml

More than after two kinds of liquid mixing pH value be adjusted to about 7.15

Below used liquid be above PBS buffer system,

(2) preparation of novel skin reparation combination:

Material name content (g/kg)

EDTA-2Na 1.0

Glycerol 50.0

1,3 butanediol 50.0

Metallothionein white zine (MT) 5mg

Oil-solvent preservative 2.0

(buffer solution 849ml)

Heparin sodium 5.0

Recombinant human epidermal growth factor (rhEGF) 2.0mg

RhMGF (rhSCF) 1.0mg.