CN1765370A - Method for preparing anti-aftosa serum - Google Patents
Method for preparing anti-aftosa serum Download PDFInfo
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- CN1765370A CN1765370A CNA2004100111710A CN200410011171A CN1765370A CN 1765370 A CN1765370 A CN 1765370A CN A2004100111710 A CNA2004100111710 A CN A2004100111710A CN 200410011171 A CN200410011171 A CN 200410011171A CN 1765370 A CN1765370 A CN 1765370A
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- mouth disease
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/70—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in livestock or poultry
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- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
The invention relates to a process for preparing foot-and-mouth disease resisting serum, which comprises immunizing livestock by utilizing foot-and-mouth disease inactivated vaccine so as to produce antiserum, finally sampling blood and separating the antiserum.
Description
Technical field
The invention belongs to field of biological product, be specifically related to the preparation method of anti-foot and mouth disease serum
Background technology
Foot and mouth disease (FMD) is a kind of acute deadly infectious disease of ruminant that is caused by foot and mouth disease virus (FMDV).Foot and mouth disease infection rate and sickness rate are all high, about 3 days incubation periods.The hobby tissue of foot and mouth disease virus is the hoof hairiness and the epithelial tissue and the oral mucosa cell that do not have the hair intersection, and replication-competent virus particle in cytoplasm is interconnected into blister after making cell impaired, occurs lapsing to and toxin expelling after the symptom.
The method of the anti-foot and mouth disease of using has at present: foot and mouth disease inactivated vaccine inoculation animal makes animal produce antibody; Also useful Chinese medicine preparation treatment and prevention foot and mouth disease.
Behind foot and mouth disease inactivated vaccine inoculation animal, the time more than 15 days, just produce stronger immunity, but protective rate has only about 80%.With Chinese medicine preparation treatment and prevention foot and mouth disease, also produce little effect.China stock raising amount is big in addition, dispersion, epidemic prevention system are unsound, vaccine supply and force inoculation to be difficult to implement, and vaccine quality is still waiting to improve.When epidemic situation took place, to non-immune domestic animal, the domestic animal that the foot-and-mouth disease antibody level is low was in preclinical domestic animal, vaccination but antibody horizontal does not also reach the domestic animal of antibody protection value, and the herd that the vaccine protective rate is low will produce serious harm.
Anti-foot and mouth disease serum has overcome above-mentioned existing anti-foot and mouth disease technical deficiency part, can be used for when epidemic situation takes place to non-immune domestic animal urgent prevention and the treatment of the domestic animal that the foot-and-mouth disease antibody level is low.Also can be used for the preventive use of domestic animal in long-distance transport.
Summary of the invention
The preparation method that the purpose of this invention is to provide a kind of anti-foot and mouth disease serum.
The inventive method is to realize with step by following technical solution: 1) select healthy domestic animal, comprising: pig, horse, cattle; 2) with the foot and mouth disease inactivated vaccine domestic animal is carried out fundamental immunity, immunization route is that neck, back are subcutaneous, injection twice, 3ml for the first time, 6 points, every some 0.5ml; 14 days at interval, inject 6ml, 6 points, every some 1ml for the second time; 3) with the foot and mouth disease inactivated vaccine domestic animal is carried out reinforced immunological, the beginnings in 21 days after fundamental immunity is finished, immunization route is neck, subcutaneous, the muscle in back.Inject three times, inject 7ml, subcutaneous 4 points, 3 points of muscle, every some 1ml for the first time; 5-7 days at interval, inject 11ml for the third time, subcutaneous 4 points, 2 points of muscle, subcutaneous 2 points of basal part of the ear portion, 1 point of Houhai acupoint, 2 points of shoulder lymph node, every some 1ml; 4) antiserum titre is measured with forward indirect hemagglutination (IHA) experiment box and liquid phase blocking-up enzyme-linked immunosorbent assay (LB-ELISA) experiment box in the 10th day behind the reinforced immunological; 5) blood sampling, (LBE 〉=3.01 (1/1024) are during IHA 〉=11log2) when antibody titer reaches peak value, about 12-15 days, carry out the blood sampling first time, carried out taking a blood sample 6 the second time in 3-5 days at interval) the serum separation, adopting the nature pressurization 7 of condensing) antiserum extracts, and adopts sad extraction method.
The specific embodiment
1. select health pig
2. with the foot and mouth disease inactivated vaccine pig is carried out fundamental immunity, immunization route is that neck, back are subcutaneous, injection twice, 3ml for the first time, 6 points, every some 0.5ml; 14 days at interval, inject 6ml, 6 points, every some 1ml for the second time;
3. with the foot and mouth disease inactivated vaccine pig is carried out reinforced immunological, the beginnings in 21 days after fundamental immunity is finished, immunization route is neck, subcutaneous, the muscle in back.Inject three times, inject 7ml, subcutaneous 4 points, 3 points of muscle, every some 1ml for the first time; 5-7 days at interval, inject 11ml for the third time, subcutaneous 4 points, 2 points of muscle, subcutaneous 2 points of basal part of the ear portion, 1 point of Houhai acupoint, 2 points of shoulder lymph node, every some 1ml;
4. antiserum titre is measured with forward indirect hemagglutination (IHA) experiment box and liquid phase blocking-up enzyme-linked immunosorbent assay (LB-ELISA) experiment box in the 10th day behind the reinforced immunological;
5. blood sampling, when antibody titer reach peak value (LBE 〉=3.01 (1/1024), during IHA 〉=11log2), about 12-15 days, carry out blood sampling first time, the interval was carried out the second time in 3-5 days and is taken a blood sample
6. serum separates, and adopts the nature pressurization of condensing
7. antiserum extracts, and adopts sad extraction method
(1) waits the product of getting sample (serum), with 60m mol/L ph4.0 acetate buffer solution 3-4 doubly, transfer ph to 4.5;
(2) room temperature, the powerful limit of stirring, limit adds sad 25 μ l/ml, stirs 30-40 ';
(3) the centrifugal 30 ' qualitative filter paper of 10000Xg filters, and gets filtrate;
(4) add 1 times of volume PBS, transfer PH to 7.4;
(5) ice bath is molten to 4 ℃, after 0.27g ammonium sulfate/ml mixes, and stirring 30 ';
(6) 5000xg centrifugal 15 ', collecting precipitation is dissolved among a small amount of PBS;
(7) 1500xg centrifugal 20 '.Get supernatant, filter promptly with the doughnut filtration system.
Claims (2)
1, the preparation method of anti-foot and mouth disease serum for animals such as a boar, cattle is characterized in that adopting the foot and mouth disease inactivated vaccine that domestic animal is carried out immunity, produces antiserum, and blood sampling also separates and obtains antiserum.
2, the preparation method of anti-foot and mouth disease serum according to claim 1 is characterized in that described method comprises the steps 1) select healthy domestic animal, comprising: pig, horse, cattle; 2) with the foot and mouth disease inactivated vaccine domestic animal is carried out fundamental immunity, immunization route is that neck, back are subcutaneous, injection twice, 3ml for the first time, 6 points, every some 0.5ml; 14 days at interval, inject 6ml, 6 points, every some 1ml for the second time; 3) with the foot and mouth disease inactivated vaccine domestic animal is carried out reinforced immunological, the beginnings in 21 days after fundamental immunity is finished, immunization route is neck, subcutaneous, the muscle in back.Inject three times, inject 7ml, subcutaneous 4 points, 3 points of muscle, every some 1ml for the first time; 5-7 days at interval, inject 11ml for the third time, subcutaneous 4 points, 2 points of muscle, subcutaneous 2 points of basal part of the ear portion, 1 point of Houhai acupoint, 2 points of shoulder lymph node, every some 1ml; 4) antiserum titre is measured with forward indirect hemagglutination (IHA) experiment box and liquid phase blocking-up enzyme-linked immunosorbent assay (LB-ELISA) experiment box in the 10th day behind the reinforced immunological; 5) blood sampling, (LBE 〉=3.01 (1/1024) are during IHA 〉=11log2) when antibody titer reaches peak value, about 12-15 days, carry out the blood sampling first time, carried out taking a blood sample 6 the second time in 3-5 days at interval) the serum separation, adopting the nature pressurization 7 of condensing) antiserum extracts, and adopts sad extraction method.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2004100111710A CN1765370A (en) | 2004-10-26 | 2004-10-26 | Method for preparing anti-aftosa serum |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2004100111710A CN1765370A (en) | 2004-10-26 | 2004-10-26 | Method for preparing anti-aftosa serum |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1765370A true CN1765370A (en) | 2006-05-03 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2004100111710A Pending CN1765370A (en) | 2004-10-26 | 2004-10-26 | Method for preparing anti-aftosa serum |
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| CN (1) | CN1765370A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105675866A (en) * | 2016-04-07 | 2016-06-15 | 郑州中道生物技术有限公司 | Solid-phase blocked ELISA (Enzyme Linked Immune Sorbent Assay) kit for detecting O-type FMDV (Foot and Mouth Disease Virus) antibody |
-
2004
- 2004-10-26 CN CNA2004100111710A patent/CN1765370A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105675866A (en) * | 2016-04-07 | 2016-06-15 | 郑州中道生物技术有限公司 | Solid-phase blocked ELISA (Enzyme Linked Immune Sorbent Assay) kit for detecting O-type FMDV (Foot and Mouth Disease Virus) antibody |
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| WD01 | Invention patent application deemed withdrawn after publication |