CN115814073A - Pig multivalent immunoglobulin and preparation method thereof - Google Patents

Abstract

The invention discloses a pig multivalent immunoglobulin and a preparation method thereof, and belongs to the technical field of biology. The invention adopts the porcine virus (bacteria) vaccine as an antigen, induces a humoral immune system of the xenogeneic equine animal to generate high-efficiency multivalent immunoglobulin after being inoculated to the xenogeneic equine animal, purifies the immunoglobulin through enzyme digestion, salting out, filtration, ultrafiltration molecular interception and other biological technologies, standardizes and industrializes the concentration and refining process of the multivalent immunoglobulin, accords with biological safety, and achieves the emergency prevention and the emergency treatment of the swine diseases, and the cure rate of the swine disease reaches more than 95 percent at the early stage of the disease.

Description

Pig multivalent immunoglobulin and preparation method thereof

Technical Field

The invention belongs to the technical field of vaccines, and particularly relates to porcine multivalent immunoglobulin and a preparation method thereof.

Background

According to the epidemic situation of swine diseases in recent years in China, the prevention and control situation is complex, epidemic diseases are increased continuously, pathogen variation is accelerated, the toxicity is enhanced, particularly, the change of genes of the porcine reproductive and respiratory syndrome and the threat of immunosuppressive diseases are aggravated continuously, and the porcine reproductive and respiratory syndrome, the porcine reproductive and respiratory syndrome and the like destroy lymphocytes of an organism, so that the immunity is reduced, a swine herd is kept in a sub-health threat state after being subjected to immunosuppression, and the antibody level of the organism is influenced after the injection of vaccines of the swine fever, the porcine pseudorabies and the like. Therefore, the pig raising people are very painful in prevention and control, are easy to be infected with respiratory diseases, and are more and more common in mixed infection of various pathogens, such as infection among viruses, mixed infection among porcine reproductive and respiratory syndrome viruses and porcine circovirus, mixed infection among porcine reproductive and respiratory syndrome viruses, mixed infection among bacteria, viruses and parasites, and the like.

At present, diseases are frequently generated, outbreaks occur frequently, mass outbreaks do not have obvious seasonality, and the method is closely related to daily production management and feeding processes. The pig herd is infected for a long time and the basic immunity is low, so the awareness of herd immunity and epidemic prevention is required to be strengthened. In order to reduce the economic loss caused by epidemic diseases, the complicated today, pig virus (bacteria) vaccine is taken as antigen, after the antigen is inoculated to the heterogeneous equine, the humoral immune system is induced to generate high-efficiency multivalent immunoglobulin, then the multivalent immunoglobulin is purified by the biological technologies of enzyme digestion, salting out, filtration, ultrafiltration molecular interception and the like, the swine herd is immunized, and the vertical transmission and the emergency treatment of the epidemic diseases are prevented.

In the prior art, when the immunoglobulin is prepared, a virus strain is adopted to directly immunize an animal, so that the biological safety risk possibly exists; the low-temperature ethanol process of the existing immunoglobulin separation and purification process adopts a three-time low-temperature ethanol method to purify immunoglobulin, has the defects of multiple steps, low temperature required by the operation environment, high energy consumption and flammability and explosiveness of an ethanol solvent, needs production operation in an explosion-proof workshop when a large amount of products are put into production, and has lower product yield of the low-temperature ethanol process. In addition, the separation process is carried out at low temperature, so that the energy consumption is increased, and technical workers can easily cause occupational diseases of rheumatoid arthritis when working in a low-temperature environment for a long time.

Disclosure of Invention

One of the purposes of the invention is to provide a preparation method of porcine multivalent immunoglobulin, which adopts a swine fever live vaccine, a porcine reproductive and respiratory syndrome live vaccine, a pseudorabies live vaccine, a porcine circovirus type 2 inactivated vaccine and a porcine foot-and-mouth disease type O type A bivalent inactivated vaccine as antigens and inoculates heterogeneous equine animals at the same time to induce a humoral immune system of the heterologous equine animals to generate the multivalent immunoglobulin.

Preferably, the process of simultaneously vaccinating the xenogeneic equine animal with the antigen comprises a basal immunization and a hyperimmunization.

More preferably, the basic immunity is: the first injection of basic immunization antigen is 5 times of each pig head, the second injection is carried out after 7 days, the second injection is 20 times of each pig head, subcutaneous injection or intramuscular injection is carried out on the neck and the back, SN antibody determination is carried out by sampling blood for 14 days, horses producing SN antibodies can be used, and high immunization can be carried out after 1 month of basic immunization.

More preferably, the hyperimmunization is:

(1) First-range high immunity: injecting for 3 times, wherein the interval is 7 days between each time, the 1 st needle and the 2 nd needle are used for injecting antigen with the dosage 10 times of that of each pig head, and the 3 rd needle is used for injecting antigen with the dosage 20 times of that of each pig head; and (3) simultaneously carrying out blood test for the second injection and the third injection, measuring the antibody titer of SN in serum, wherein the titer of an indirect hemagglutination antibody detection method is more than or equal to 1:512 is qualified, and the qualified horses are subjected to blood sampling for 12 to 14 days;

(2) Exemption of the second and subsequent courses: after the first blood collection, the horse takes a rest for 7 days, the following high immunity can be carried out, the injection is carried out for 2 times, each time the interval is 7 days, the first injection is carried out according to 20 times of each pig head, the second injection is carried out according to 40 times of each pig head after the interval is 7 days, the blood test is carried out simultaneously, the antibody titer of SN in serum is measured, the first blood collection is carried out on the 12 th to 14 th days after the immunization is finished, the second blood collection is carried out at the interval of 2 days, the blood collection amount is 17 ml/Kg (ml/Kg) of body weight, the second blood collection amount is reduced by 300 ml to 400ml compared with the first blood collection amount, the blood is collected by jugular veins in a blood collection bottle containing 5 percent sodium citrate solution, and the blood plasma is collected after the collected blood precipitates form a clear yellow layer;

(3) Conventional hyperimmunization: the first 20 times of antigen injection per pig head, the second 40 times of antigen injection per pig head, the first blood collection 12-14 days after immunization, the second blood collection 2 days after immunization, the blood collection amount is 17-18ml/Kg body weight, the second blood collection amount is reduced by 300-400ml compared with the first blood collection amount, the second blood collection amount is collected in a blood collection bottle containing 5% sodium citrate solution by jugular vein blood collection, and the indirect hemagglutination antibody titer detection method is more than or equal to 1:512 is qualified, and after the qualified blood precipitates to form a clear yellow layer, the blood plasma is collected and absorbed and stored at the temperature of 2-8 ℃.

More preferably, the method also comprises the following steps of treating the plasma:

(1) Mixing the blood plasma to be refined and purified in the same container, adding water for injection according to 2 times of the amount of the blood plasma, fully and uniformly stirring, adjusting the pH value to 3.3-3.4 by using 2mol/L hydrochloric acid, then adding 6-9 active pepsins and 0.2% toluene according to the total amount per milliliter, uniformly stirring, heating in a water bath at 30 +/-1 ℃ for digesting for 2 hours, and continuously stirring the purified blood plasma during the digestion;

(2) Adding 15% ammonium sulfate according to the total amount of the plasma after digestion, heating with 2N NaOH solution to adjust the pH value to 4.5-4.7 by water bath method, raising the temperature to 1 ℃ per minute, continuously stirring until the temperature is raised to 57-58 ℃, stirring for 30min, standing for 30min, and carrying out the next step of process treatment when the plasma is cooled to below 45 ℃;

(3) The precipitate was filtered until clear, the filtrate was collected and the precipitate discarded.

More preferably, the method further comprises the step of performing ultrafiltration interception treatment on the collected filtrate:

(1) Removing low molecular weight substances by adopting a 40DK ultrafiltration membrane: using an ultrafilter provided with a 40DK ultrafiltration membrane, and circularly cleaning with 0.4% sodium hydroxide solution for 30 minutes under the pressure of not more than 0.1MPa; washing the concentrated solution and the ultrafiltrate with water for injection, directly discharging, discharging the liquid in the ultrafilter after the pH value of the liquid discharged from the ultrafiltrate is neutral, putting into a normal ultrafiltration program, adjusting the flow rate of the ultrafiltrate as required at the beginning of ultrafiltration, controlling the pressure not to exceed 0.1MPa, leaving the concentrated solution, eliminating the ultrafiltrate until the concentrated solution is three-fourth of the amount of the input plasma, adding the same amount of water for injection, and continuing to ultrafilter until the concentrated solution is three-fourth of the input plasma; washing and ultrafiltering with water for injection for five times to obtain concentrated solution (I);

(2) Removing high molecular weight substances by adopting a 150DK ultrafiltration membrane: circularly cleaning an ultrafilter provided with a 150DK ultrafiltration membrane for 30 minutes by using 0.4% sodium hydroxide solution, wherein the pressure is not more than 0.1MPa; then directly discharging the concentrated solution and the ultrafiltrate by washing with water for injection, discharging the liquid in the ultrafilter after the pH value of the liquid discharged from the ultrafiltrate reaches neutral, putting into normal ultrafiltration procedure, adjusting the flow rate of the ultrafiltrate at the beginning of ultrafiltration as required, controlling the pressure not to exceed 0.1MPa, discarding the concentrated solution, and collecting the ultrafiltrate until the ultrafiltrate is nine-tenth of the concentrated solution (I), thereby obtaining the required immunoglobulin ultrafiltrate.

More preferably, the method further comprises the step of treating the immunoglobulin ultrafiltrate: adding thimerosal 0.0035% and sodium chloride 0.85% into the immunoglobulin ultrafiltrate, stirring to dissolve, adjusting pH to 6.6-6.8 with 2N NaOH solution, sterilizing with 0.2um filter core under positive pressure, sampling, performing semi-finished product inspection, and standing at 2-8 deg.C.

More preferably, the immunoglobulin filtrate is taken, and the titer of classical swine fever per ml is more than or equal to 1: 512. porcine reproductive and respiratory syndrome per milliliter is more than or equal to 1: 16. porcine circovirus per ml is more than or equal to 1: 256. the foot-and-mouth disease per ml of the pig is more than or equal to 1: 512. every ml of porcine pseudorabies is more than or equal to 1: and 16, proportioning, sterilizing and subpackaging, and inspecting the finished product to be qualified for use.

The second purpose of the invention is to provide the porcine multivalent immunoglobulin prepared by the preparation method.

Compared with the prior art, the invention has the following beneficial effects:

(1) The equine animal, which is an animal resource used for preparing the multivalent immunoglobulin, has the advantages of little epidemic disease, strong disease resistance, rich animal sources, easy captivity, large plasma yield, easy recovery of physique after blood is taken twice a month, easy barn feeding, low allergic reaction to animals and the like, and is easy to standardize and industrially produce biological products. Compared with the large donkey in Guandong, the donkey is more in equine animals, easier to buy and more convenient to feed.

(2) The immunogen directly adopts a vaccine product which is produced by a qualified vaccine manufacturer approved by the state and has an approved code, and is not directly prepared by virus liquid or bacterial liquid and the BCG, so that the risk of spreading and diffusing epidemic diseases caused by immunity is avoided, the safety is higher, and the effect of generating antibodies by immunity is better.

(3) The immunization program adopts basic immunization for 2 times at intervals of 7 days, the titer is detected, and horses capable of producing antibodies are selected for the next immunization. Hyperimmunization used two course of immunization: hyperimmunity was performed 3 times with 7 days intervals. The immune dose is gradually increased to stimulate animals to generate antibodies, the 2 nd to 3 rd processes of immunity are carried out while the blood test titer is tested, and qualified horses are subjected to blood collection 12 to 14 days after the last process; hyperimmunity was performed 2 times with 7 days intervals. Compared with 6 times of basic immunization and 2 times of hyperimmunity, the method increases the hyperimmunity blood sampling of the horses with excellent antibody production by basic immunization selection, shortens the hyperimmunity times and time, adjusts the immunization blood sampling for 12-14 days, and collects blood when the highest gradient of the antibody production of animals can be reached.

(4) The biological product of the invention is transparent clear liquid, and the content of multivalent immunoglobulin can be detected and quantified. After being treated by a sterilizer, the product can be stored for two years at normal temperature (2-10 ℃), and has stable properties, no reduction in efficacy, no reduction in curative effect and guaranteed quality.

(5) The preparation method adopts enzyme digestion-salting out-ultrafiltration molecular carrying, so that the average immunoglobulin content in the preparation is improved by about 1-2 times, and the titer in unit volume is stable. The secondary precipitation and the running water dialysis process in the ammonium sulfate precipitation method are replaced by adding the ultrafiltration molecular interception process: ultrafiltration interception of the low molecular weight 40DK removes small molecular substances including Fc fragments and the like; the ultrafiltration of 150DK with high molecular weight removes macromolecular substances, more effectively retains the needed immunoglobulin molecular substances, greatly improves the purity of the immunoglobulin, shortens the purification process time, avoids heat source substances brought to products in the dialysis process, greatly reduces the anaphylactic reaction and heat source reaction of animal organisms in clinical application, and has more obvious and more targeted clinical treatment effect.

(6) The existing immunoglobulin separation and purification process also has a low-temperature ethanol process, the immunoglobulin is purified by adopting a three-time low-temperature ethanol method, the defects of multiple steps, low temperature required by the operation environment, high energy consumption and flammability and explosiveness of an ethanol solvent exist, the production operation in an explosion-proof workshop is required for mass production, and the product yield of the low-temperature ethanol process is lower. In addition, the separation process is carried out at low temperature, so that the energy consumption is increased, and technical workers can easily cause occupational diseases of rheumatoid arthritis when working in a low-temperature environment for a long time.

(7) The product only contains 0.0035% of thimerosal, and does not need gentamycin sulfate and chloroform.

(8) The sick animals can be cured by three days, can be recovered basically without sequela, and can also be prevented by sows and boars before mating, the viremia and the vertical transmission generated by the viremia can be prevented, and the pregnant sows can lay healthy piglets without other toxic and side effects. The traditional Chinese medicine composition can be used for purifying and emergently treating epidemic diseases such as swine fever, porcine reproductive and respiratory syndrome, porcine circovirus disease, porcine foot and mouth disease, porcine pseudorabies and the like, and the cure rate can reach more than 95% at the early stage of disease.

Drawings

FIG. 1 is a flow chart of the preparation of the immunoglobulin of the present invention.

Detailed Description

Example 1

When the multivalent immunoglobulin is used for preparing the pig, virus (bacteria) vaccines for hog cholera, porcine reproductive and respiratory syndrome, porcine circovirus, porcine foot and mouth disease, porcine pseudorabies and the like are adopted as antigens, and the heterologous equine animal is artificially immunized. The various vaccine formulations are shown in table 1.

TABLE 1

(1) Basic immunity: and (4) performing two basic immunizations on the horses which are qualified through inspection. The primary immunization with the primary immunization antigen was performed in 5-fold amount per pig portion, and the 2 nd vaccination with the primary immunization antigen was performed 7 days later (20-fold amount per pig portion). Injecting subcutaneously or intramuscularly at the back of the neck, and sampling blood for 14 days to determine SN antibody, which is a parameter basis for high hands-free. Horses producing SN antibodies can be hyperimmunized 1 month after completion of the basic immunization.

(2) High immunity: the antigen injections, immunization methods and procedures were performed as in table 2.

TABLE 2 hyperimmunization program table

(1) First-range high immunity: the injection is carried out for 3 times, the interval is 7 days, the 1 st needle and the 2 nd needle are used for injecting antigen dose which is 10 times of the amount of each pig head, and the 3 rd needle is used for injecting antigen dose which is 20 times of the amount of each pig head. And (3) simultaneously carrying out blood test for the second injection and the third injection, measuring the antibody titer of SN in serum, and measuring the titer of an indirect hemagglutination antibody detection method to be more than or equal to 1:512 is qualified, and the qualified horses are subjected to blood sampling for 12-14 days.

(2) Exemption of the second and subsequent courses: after the first blood sampling, the horse is rested for 7 days, the following high immunity can be performed, 2 times of injection are performed, the interval of each time is 7 days, the injection antigen dosage can be increased and decreased according to the previous serum titer, the first injection is 20 times of the quantity of each part of the pig, and the second injection is 40 times of the quantity of each part of the pig every 7 days. The injection is performed with blood test, and the SN antibody titer in the serum is determined. The first blood collection is carried out 12-14 days after the immunization is finished, and the second blood collection is carried out at an interval of 2 days. The blood collection amount is 17-18ml/Kg body weight, the second blood collection amount is reduced by about 300-400ml compared with the first blood collection amount, the blood is collected by jugular vein in a blood collection bottle containing 5% sodium citrate solution, and the blood is collected and absorbed after the collected blood precipitates to form a clear yellow layer.

(3) After immune horses enter hyperimmunity, special attention should be paid to their health status, the raising management should be strengthened, when diseases or possible infectious diseases occur, injection and blood sampling should be stopped immediately, and isolated observation should be carried out, and necessary measures should be taken.

(4) Conventional hyperimmunization (2 needles): typically 2 injections are given. The antigen is injected for the first time in an amount which is 20 times that of each pig head part per dose, and the antigen is injected for the second time in an amount which is 40 times that of each pig head part per dose at intervals of 7 days. The first blood collection is carried out 12-14 days after the immunization is finished, and the second blood collection is carried out at an interval of 2 days. The amount of blood collected is 17-18ml/Kg of body weight, the amount of blood collected in the second time is reduced by about 300-400ml compared with the first time, the blood is collected by jugular vein in a blood collection bottle containing 5% sodium citrate solution, and the blood is sampled and detected at the same time, and the indirect hemagglutination antibody detection and the neutralization antibody detection titer swine fever per ml are more than or equal to 1: 512. the porcine reproductive and respiratory syndrome per milliliter is more than or equal to 1: 16. porcine circovirus per ml is more than or equal to 1: 256. the foot-and-mouth disease per ml of the pig is more than or equal to 1: 512. the porcine pseudorabies per ml is more than or equal to 1: and 16 is qualified, and after the qualified blood precipitates to form a clear yellow layer, collecting and sucking blood plasma, and storing at 2-8 ℃.

Example 2 purification of immunoglobulin (Ig)

1. The plasma prepared in the example 1 is mixed in the same container, the injection water is added according to 2 times of the amount of the serum (plasma), the mixture is fully and evenly stirred, the pH value is adjusted to 3.3 to 3.4 by 2moI/L hydrochloric acid, then 6 to 9 active pepsins and 0.2 percent toluene are added according to the total amount per milliliter, the mixture is evenly stirred, and the mixture is heated in a water bath at 30 +/-1 ℃ for digestion for 2 hours. The purified plasma is continuously stirred during digestion.

2. Adding 15% ammonium sulfate based on the total amount of plasma after digestion, dissolving, adjusting pH to 4.5-4.7 with 2N NaOH solution, heating with water bath method at 1 deg.C per minute under stirring, heating to 57-58 deg.C, stirring for 30min, standing for 30min, and cooling plasma below 45 deg.C for further processing.

3. Filtering the precipitate, filtering the above liquid with filter cloth in a filter tank (or filter press), collecting filtrate, and discarding the precipitate.

4. And (3) carrying out ultrafiltration interception process on the collected filtrate:

(1) And (3) removing low molecular weight substances by adopting a 40DK ultrafiltration membrane: circularly cleaning an ultrafilter provided with a 40DK ultrafiltration membrane for 30 minutes by using 0.4% sodium hydroxide solution, wherein the pressure is not more than 0.1MPa; and (3) directly discharging the concentrated solution and the ultrafiltrate by using the injection water, discharging the liquid in the ultrafilter after the pH value of the liquid discharged from the ultrafiltrate reaches neutral about 40 minutes, and putting the liquid into a normal ultrafiltration procedure. Adjusting the flow rate of the ultrafiltrate at the beginning of ultrafiltration, controlling the pressure not to exceed 0.1MPa, and leaving the concentrated solution to eliminate the ultrafiltrate. Until the concentrate is three-quarters of the amount of plasma dosed. Adding equal amount of water for injection, and continuously ultrafiltering until the concentrated solution is three-fourths of the added blood plasma; the ultrafiltration was repeated five times with water for injection to obtain a concentrate (I).

(2) Removing high molecular weight substances by adopting a 150DK ultrafiltration membrane: using an ultrafilter provided with a 150DK ultrafiltration membrane, and circularly cleaning with 0.4% sodium hydroxide solution for 30 minutes under the pressure of not more than 0.1MPa; and (4) directly discharging the concentrated solution and the ultrafiltrate by using the water for injection for about 40 minutes, discharging the liquid in the ultrafilter after the pH value of the liquid discharged from the ultrafiltrate reaches neutral, and putting into a normal ultrafiltration procedure. Adjusting the flow rate of the ultrafiltrate at the beginning of ultrafiltration, controlling the pressure not to exceed 0.1MPa, discarding the concentrated solution (II), and collecting the ultrafiltrate. Until the ultrafiltrate is nine tenth of the concentrated solution (I), the required immunoglobulin ultrafiltrate is obtained.

5. Adding thimerosal 0.0035% and sodium chloride 0.85% into the immunoglobulin ultrafiltrate, stirring to dissolve, and adjusting pH to 6.6-6.8 with 2N NaOH solution. Sterilizing and filtering with 0.2um filter core under positive pressure, sampling, inspecting semi-finished product, standing at 2-8 deg.C.

6. And (5) after the semi-finished product is inspected to be qualified, grouping and subpackaging. Taking the semi-finished product of the immune globulin filtrate, and neutralizing the titer of the finished product of the antibody according to the ratio of hog cholera per milliliter of more than or equal to 1: 512. porcine reproductive and respiratory syndrome per milliliter is more than or equal to 1: 16. porcine circovirus per ml is more than or equal to 1: 256. the foot-and-mouth disease per ml of the pig is more than or equal to 1: 512. every ml of porcine pseudorabies is more than or equal to 1:16, sterilizing and subpackaging according to a proportion, and inspecting the finished product to be qualified for use.

Three batches of experimental products were prepared as in example 1 and example 2, and the statistical results are shown in Table 3 (80000 ml was put into production and 54000 ml of semi-finished product was obtained by purification).

TABLE 3

Test example 1

100 sows are bred in two xxx and xxx pig farms in Liaoning, and the situations of sanitation, purification and epidemic prevention measures are shown in the table 4.

TABLE 4

Test example 2

The treatment of porcine reproductive and respiratory syndrome and porcine circovirus mixed infection in a pig farm is shown in table 5:

1. 263 head of a certain pig farm in Jilin, three-element white pig in French series, weaned pigs, clinically manifested as depressed spirit, decreased feed intake, dyspnea, increased cough, dyspnea, cold intolerance, body temperature up to above 41 ℃, cyanosis of skin, loose and disorderly fur, diarrhea, conjunctivitis, orbital edema, malnutrition, general weakness, few pigs with weakness of hind body, and purple spots on the skin of ears, tail tips and limbs. Laboratory diagnosis: collecting heart blood, liver, spleen, kidney and tonsil, and detecting blue ear disease, porcine circovirus disease and swine fever by ELISA detection method. The detection result shows that the porcine reproductive and respiratory syndrome is positive, the porcine circovirus disease is positive, and the swine fever is negative.

2. Treatment: strengthening sanitation management, and treating by intramuscular injection or intravenous injection of 0.1-0.2ml per kg body weight once a day for twice a day.

TABLE 5

The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.

Claims (9)

1. A preparation method of pig multivalent immunoglobulin is characterized in that a swine fever live vaccine, a pig blue ear live vaccine, a pseudorabies live vaccine, a pig circovirus type 2 inactivated vaccine and a pig foot-and-mouth disease type A bivalent inactivated vaccine are adopted as antigens and are simultaneously inoculated to a heterogeneous equine animal, and then a humoral immune system of the heterogeneous equine animal is induced to generate the multivalent immunoglobulin.

2. The method of claim 1, wherein the antigen is administered to the xenogeneic equine animal simultaneously by a process comprising both prime and hyperimmunization.

3. The method of claim 2, wherein the basic immunization is: the first injection of basic immunization antigen is 5 times of each pig head, the second injection is carried out after 7 days, the second injection is 20 times of each pig head, subcutaneous injection or intramuscular injection is carried out on the neck and the back, SN antibody determination is carried out by sampling blood for 14 days, horses producing SN antibodies can be used, and high immunization can be carried out after 1 month of basic immunization.

4. The method of claim 3, wherein the hyperimmunization process comprises:

(1) First-range high immunity: injecting for 3 times, wherein the interval is 7 days between each time, the 1 st needle and the 2 nd needle are used for injecting antigen with the dosage 10 times of that of each pig head, and the 3 rd needle is used for injecting antigen with the dosage 20 times of that of each pig head; and (3) simultaneously carrying out blood test for the second injection and the third injection, measuring the antibody titer of SN in serum, wherein the titer of an indirect hemagglutination antibody detection method is more than or equal to 1:512 is qualified, and the qualified horses are subjected to blood sampling for 12 to 14 days;

(2) Exemption of the second and subsequent courses: after the first blood collection, the horse is rested for 7 days, then the next hyperimmunization can be carried out, the injection is carried out for 2 times, each time the injection is 7 days, the first injection is carried out according to 20 times of each pig head, the second injection is carried out according to 40 times of each pig head every 7 days, the blood is tested simultaneously, the antibody titer of SN in serum is measured, the first blood collection is carried out on 12 th to 14 th days after the immunization is finished, the second blood collection is carried out on 2 days, the blood collection amount is 17 ml/Kg (body weight), the second blood collection amount is reduced by 300 ml to 400ml compared with the first blood collection amount, the blood is collected by jugular vein in a blood collection bottle containing 5 percent sodium citrate solution, and the blood plasma is collected after the collected blood precipitates to form a clear yellow layer;

(3) Conventional hyperimmunization: the first 20 times of antigen injection per pig head, the second 40 times of antigen injection per pig head, the first blood collection 12-14 days after immunization, the second blood collection 2 days after immunization, the blood collection amount is 17-18ml/Kg body weight, the second blood collection amount is reduced by 300-400ml compared with the first blood collection amount, the second blood collection amount is collected in a blood collection bottle containing 5% sodium citrate solution by jugular vein blood collection, and the indirect hemagglutination antibody titer detection method is more than or equal to 1:512 is qualified, collecting blood plasma after the qualified blood precipitates to form clear yellow layer, and storing at 2-8 deg.C.

5. The method of claim 4, further comprising the steps of treating the plasma:

(1) Mixing the blood plasma to be refined and purified in the same container, adding water for injection according to 2 times of the amount of the blood plasma, fully and uniformly stirring, adjusting the pH value to 3.3-3.4 by using 2mol/L hydrochloric acid, then adding 6-9 active pepsins and 0.2% toluene according to the total amount per milliliter, uniformly stirring, heating in a water bath at 30 +/-1 ℃ for digesting for 2 hours, and continuously stirring the purified blood plasma during the digestion;

(2) Adding 15% ammonium sulfate according to the total amount of the plasma after digestion, heating with 2N NaOH solution to adjust the pH value to 4.5-4.7 by water bath method, raising the temperature to 1 ℃ per minute, continuously stirring until the temperature is raised to 57-58 ℃, stirring for 30min, standing for 30min, and carrying out the next step of process treatment when the plasma is cooled to below 45 ℃;

(3) The precipitate is filtered until clear, the filtrate is collected and the precipitate is discarded.

6. The method of claim 5, further comprising the step of performing ultrafiltration interception on the collected filtrate:

(1) And (3) removing low molecular weight substances by adopting a 40DK ultrafiltration membrane: using an ultrafilter provided with a 40DK ultrafiltration membrane, and circularly cleaning with 0.4% sodium hydroxide solution for 30 minutes under the pressure of not more than 0.1MPa; cleaning the concentrated solution and the ultrafiltrate with water for injection, directly discharging, discharging the liquid in the ultrafilter after the pH value of the liquid discharged from the ultrafiltrate reaches neutral, putting into normal ultrafiltration procedure, adjusting the flow rate of the ultrafiltrate at the beginning of ultrafiltration as required, controlling the pressure not to exceed 0.1MPa, leaving the concentrated solution, eliminating the ultrafiltrate until the concentrated solution is three-fourth of the input plasma, adding the same amount of water for injection, and continuing to ultrafilter until the concentrated solution is three-fourth of the input plasma; washing and ultrafiltering with water for injection for five times to obtain concentrated solution (I);

(2) Removing high molecular weight substances by adopting a 150DK ultrafiltration membrane: using an ultrafilter provided with a 150DK ultrafiltration membrane, and circularly cleaning with 0.4% sodium hydroxide solution for 30 minutes under the pressure of not more than 0.1MPa; and then directly discharging the concentrated solution and the ultrafiltrate by using the injection water, discharging the liquid in the ultrafiltrate after the pH value of the liquid discharged from the ultrafiltrate reaches neutral, putting into a normal ultrafiltration procedure, adjusting the flow rate of the ultrafiltrate at the beginning of ultrafiltration as required, controlling the pressure to be not more than 0.1MPa, discarding the concentrated solution, and collecting the ultrafiltrate until the ultrafiltrate is nine tenth of the concentrated solution (I) to obtain the required immunoglobulin ultrafiltrate.

7. The method of claim 6, further comprising the step of treating the immunoglobulin ultrafiltrate by: adding thimerosal 0.0035% and sodium chloride 0.85% into the immunoglobulin ultrafiltrate, stirring to dissolve, adjusting pH to 6.6-6.8 with 2N NaOH solution, sterilizing with 0.2um filter core under positive pressure, sampling, performing semi-finished product inspection, and standing at 2-8 deg.C.

8. The method of claim 7, wherein the immunoglobulin filtrate is extracted according to the indirect hemagglutination antibody detection and neutralization antibody detection titer hog cholera per ml of at least 1: 512. porcine reproductive and respiratory syndrome per milliliter is more than or equal to 1: 16. porcine circovirus per milliliter is more than or equal to 1: 256. the foot-and-mouth disease per ml of the pig is more than or equal to 1: 512. every ml of porcine pseudorabies is more than or equal to 1: and 16, proportioning, sterilizing and subpackaging, and inspecting the finished product to be qualified for use.

9. Porcine multivalent immunoglobulin prepared by the method of any one of claims 1 to 8.

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