CN104491860A - Pet viral egg yolk antibody oral solution and preparation method thereof - Google Patents
Pet viral egg yolk antibody oral solution and preparation method thereof Download PDFInfo
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- CN104491860A CN104491860A CN201410762706.1A CN201410762706A CN104491860A CN 104491860 A CN104491860 A CN 104491860A CN 201410762706 A CN201410762706 A CN 201410762706A CN 104491860 A CN104491860 A CN 104491860A
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Abstract
The invention discloses a pet viral egg yolk antibody oral solution and a preparation method thereof. The formula is characterized in that the pet viral egg yolk antibody oral solution comprises the following raw materials in percentage by volume: 95-105 of a whole-egg water solution containing an egg yolk antibody, 0.2-0.3 of octanoic acid, 0.2-0.3 of formaldehyde, 0.01-0.02 of merthiolate and 800-1000 units of mycillin in every milliliter of the oral solution. Whole eggs with high immunoglobulin are adopted during preparation, so that the clear egg yolk antibody oral solution is obtained. The egg yolk antibody oral solution prepared by using the preparation method is not only low in loss, high in immune active substance content, high in biological activity and free of clinical side effects in the production process, but also contains a great number of various proteins in egg white, so that the adverse effects on the antibody can be reduced during oral administration.
Description
Technical field
The invention belongs to pig biological product technical field, relate to viral yolk antibody oral liquid of a kind of anti-house pet and preparation method thereof.
Background technology
Treating viral diseases of pets comprises the viral disease such as canine parvovirus, canine distemper, this virosis clinical epidemiological features such as sickness rate is high clinically, the dead rate of height, infectiousness are high, along with people strengthen the dependency of house pet, also be improve to the treatment level of house pet disease always, if this yolk antibody can be carried out, so can reduce clinically to the drug use of this kind of disease of animal, also can reduce the stimulation to animal simultaneously.
A kind of Mitochondrion IgG that yolk antibody IgY is, IgY is functionally equivalent to mammal IgG, but different in structure, has the space conformation of typical immunoglobulin.Yolk antibody has lot of superiority.(1) due to the gap of birds and mammal phylogenetics, birds are more suitable for the specific antibody of producing resisting mammal antigen.Yolk antibody can not excite complement system, does not react with the antibody of resisting mammal antigen, and not with the Fc receptors bind of mammal and antibacterial, this is significant in immunologic diagnosis.(2) yolk antibody has bioactive immunoglobulin as one, is storing, produces, processes, ingests and ensureing in digestion process that its stability is very crucial.Multitest shows, IgY has good stability, acidproof, alkaline-resisting, heat-resisting.Yolk antibody is more easily preserved, and deposits 6 months antagonist activity and have no significant effect under 4 DEG C of conditions under depositing 5 years or room temperature condition.
Summary of the invention
The invention provides viral yolk antibody oral liquid of a kind of anti-house pet and preparation method thereof, solve the viral yolk antibody inconvenient problem with use of anti-house pet of injection.
The object of the invention is to be realized by following form:
The viral yolk antibody oral liquid of a kind of anti-house pet, comprises the following raw material calculated according to volume ratio: containing the shell egg aqueous solution 95-105 of yolk antibody, sad 0.2-0.3, formaldehyde 0.2-0.3, thimerosal 0.01-0.02; Also comprise the mycillin of 800-1000 units per ml oral liquid.
Preparation process is:
1) the viral inactivated vaccine of house pet is adopted to carry out reinforced immunological to chicken: adopt three immune programme for children, every minor tick 10 days, every each vaccine consumption of chicken is 2ml, when antibody titer reaches 1:(128-256) time, carry out high-immunity egg collection;
2) 0.1-0.2% bromo geramine is used to carry out disinfection to high-immunity egg;
3) by 2) in the egg pulp of high-immunity egg carry out emulsifying, emulsification times 7-10min, then add pH be 4.2-4.3, temperature is the hcl acidifying water of 4-6 DEG C, the addition of hcl acidifying water is 5.5-6.5 times of egg pulp volume, after mixing, be cooled to 3-5 DEG C, leave standstill 18-24 hour;
4) get 3) in the supernatant, be warmed up to 25-32 DEG C, it is sad to add, stir 1-2h, leave standstill 10-12h, filter; Wherein sad volume: supernatant volume is (0.2-0.3): (95-105);
5) formaldehyde, thimerosal, mycillin is added, make the shell egg aqueous solution of yolk antibody: sad: formaldehyde: the volume ratio of thimerosal is (95-105): (0.2-0.3): (0.2-0.3): (0.01-0.02), and the content of mycillin is 800-1000 units per ml oral liquid;
6) subpackage, preserves.
Filtered by the filter membrane of 1.0mm, 0.45mm, 0.22mm successively when step 4) is filtered.
The present invention is by clarifying yolk antibody oral liquid to obtaining after the extraction of high-immunity egg shell egg, low temperature acidification, sad process.Shell egg uses in the extraction of yolk antibody by the present invention, can directly orally use clinically, containing a large amount of different kinds of proteins in Ovum Gallus domesticus album, the value of high-immunity egg can be made full use of, improve immunity and the stress ability of animal body, bioavailability is high, has the impregnable advantage of biological activity.
Detailed description of the invention
Embodiment 1:
The formula of anti-house pet viral yolk antibody oral liquid comprises following raw material: containing shell egg aqueous solution 95 L of yolk antibody, sad 0.2 L, formaldehyde 0.3 L, thimerosal 0.02 L, and the mycillin of 1000 units per ml oral liquids.
Its preparation method comprises the following steps:
1) adopt commercially available existing commercially available canine parvovirus to carry out reinforced immunological, every each vaccine consumption of chicken is 2ml, and every minor tick 10 days, carries out three immune programme for children, when antibody titer reaches 1:256, starts to collect high-immunity egg.
2) high-immunity egg will be collected arrange, 0.2% bromo geramine sterilization, for subsequent use.
3) water dilution method acidification: by 2) step high-immunity egg shell egg squeeze into carry out in Pasteur's tank body fully emulsified, emulsification times 7min, then the egg pulp after emulsifying is added the hcl acidifying water that pH is 4.2, temperature is 4 DEG C, hcl acidifying water addition is 6 times of egg pulp volume, mixing, place in 4 DEG C of environment and staticly settle for 18 hours, get supernatant; Wherein, hcl acidifying water in water, adds hydrochloric acid make its pH value reach 4.2.
4) sad process; By the supernatant of step 3), the shell egg aqueous solution namely containing yolk antibody weighs, and be warmed up to 25 DEG C, it is sad to add, and fully mixes 1h, then leaves standstill 10h; Wherein, by volume, the supernatant: sad=95:0.2;
5) step 4) gained clear liquor is used successively to the membrane filtration of 1.0mm, 0.45mm, 0.22mm, leave and take filtrate;
6) to step 5) gained filtrate adds formaldehyde, thimerosal, mycillin; By volume, containing the shell egg shell egg aqueous solution of yolk antibody: sad: formaldehyde: thimerosal=95:0.2:0.3:0.02; The content of mycillin in this oral liquid is 1000 units per ml;
7) subpackage, preserves.
For determining yolk antibody oral liquid placement effect duration at normal temperatures, do following test: this product be kept in 37 DEG C of incubators, preserve 24 months, 1 week, 2 weeks respectively, January, February, April, August, December, after 16 months, 20 months, 24 months, by agar diffusion (AGP) method, the yolk antibody measuring each point in time sampling product is tired change, for Canine Parvovirus, the results are shown in Table 1.
Table 1. room temperature places the change that oral liquid different time sections is tired
result shows:yolk antibody can deposit 16 months under the preservation of room temperature.
Quality standard:
[character] this product is weak yellow liquid.
[steriling test] is undertaken by " Chinese veterinary pharmacopoeia ", asepsis growth.
[mycoplasma inspection] is undertaken by " Chinese veterinary pharmacopoeia ", grows without mycoplasma.
[exogenous virus inspection] is undertaken by " Chinese veterinary pharmacopoeia ", conforms with the regulations.
[safety verification] with 7 Japanese instar chickling 5, each oral 5 plumage parts, observe 2 weeks, all should be good for and live.
Embodiment 2:
The formula of anti-house pet viral yolk antibody oral liquid comprises following raw material: containing shell egg aqueous solution 105 L of yolk antibody, sad 0.2 L, formaldehyde 0.2 L, thimerosal 0.01 L, and the mycillin of 800 units per ml oral liquids.
Its preparation method comprises the following steps:
1) adopt commercially available existing commercially available canine parvovirus to carry out reinforced immunological, every each vaccine consumption of chicken is 2ml, and every minor tick 10 days, carries out three immune programme for children, when antibody titer reaches 1:128, starts to collect high-immunity egg.
2) high-immunity egg will be collected arrange, 0.1% bromo geramine sterilization, for subsequent use.
3) water dilution method acidification: by 2) step high-immunity egg shell egg squeeze into carry out in Pasteur's tank body fully emulsified, emulsification times 10min, then the egg pulp after emulsifying is added the hcl acidifying water that pH is 4.3, temperature is 6 DEG C, hcl acidifying water addition is 5.5 times of egg pulp volume, mixing, place in 3 DEG C of environment and staticly settle for 24 hours, get supernatant; Wherein, hcl acidifying water in water, adds hydrochloric acid make its pH value reach 4.3.
4) sad process; By the supernatant of step 3), the shell egg aqueous solution namely containing yolk antibody weighs, and be warmed up to 32 DEG C, it is sad to add, and fully mixes 2h, then leaves standstill 12h; Wherein, by volume, the supernatant: sad=105:0.2;
5) step 4) gained clear liquor is used successively to the membrane filtration of 1.0mm, 0.45mm, 0.22mm, leave and take filtrate;
6) to step 5) gained filtrate adds formaldehyde, thimerosal, mycillin; By volume, containing the shell egg shell egg aqueous solution of yolk antibody: sad: formaldehyde: thimerosal=105:0.2:0.2:0.01; The content of mycillin in this oral liquid is 800 units per ml;
7) subpackage, preserves.
For determining yolk antibody oral liquid placement effect duration at normal temperatures, do following test: this product be kept in 37 DEG C of incubators, preserve 24 months, 1 week, 2 weeks respectively, January, February, April, August, December, after 16 months, 20 months, 24 months, by agar diffusion (AGP) method, the yolk antibody measuring each point in time sampling product is tired change, the results are shown in Table 1.For Canine Parvovirus, the results are shown in Table 2.
Table 2. room temperature places the change that injection different time sections is tired
result shows:yolk antibody can deposit 16 months under the preservation of room temperature.
Quality standard:
[character] this product is weak yellow liquid.
[steriling test] is undertaken by " Chinese veterinary pharmacopoeia ", asepsis growth.
[mycoplasma inspection] is undertaken by " Chinese veterinary pharmacopoeia ", grows without mycoplasma.
[exogenous virus inspection] is undertaken by " Chinese veterinary pharmacopoeia ", conforms with the regulations.
[safety verification] with 7 Japanese instar chickling 5, each oral 5 parts, observe 2 weeks, all should be good for and live.
Embodiment 3:
The formula of anti-house pet viral yolk antibody oral liquid comprises following raw material: containing shell egg aqueous solution 100 L of yolk antibody, sad 0.3 L, formaldehyde 0.3 L, thimerosal 0.02 L, and the mycillin of 1000 units per ml oral liquids.
Its preparation method comprises the following steps:
1) adopt commercially available canine parvovirus to carry out reinforced immunological, every each vaccine consumption of chicken is 2ml, and every minor tick 10 days, carries out three immune programme for children, when antibody titer reaches 1:256, starts to collect high-immunity egg.
2) high-immunity egg will be collected arrange, 0.2% bromo geramine sterilization, for subsequent use.
3) water dilution method acidification: by 2) step high-immunity egg shell egg squeeze into carry out in Pasteur's tank body fully emulsified, emulsification times 8min, then the egg pulp after emulsifying is added the hcl acidifying water that pH is 4.3, temperature is 5 DEG C, hcl acidifying water addition is 6.5 times of egg pulp volume, mixing, place in 5 DEG C of environment and staticly settle for 20 hours, get supernatant; Wherein, hcl acidifying water in water, adds hydrochloric acid make its pH value reach 4.3.
4) sad process; By the supernatant of step 3), the shell egg aqueous solution namely containing yolk antibody weighs, and be warmed up to 30 DEG C, it is sad to add, and fully mixes 2h, then leaves standstill 11h; Wherein, by volume, the supernatant: sad=100:0.3;
5) step 4) gained clear liquor is used successively to the membrane filtration of 1.0mm, 0.45mm, 0.22mm, leave and take filtrate;
6) to step 5) gained filtrate adds formaldehyde, thimerosal, mycillin; By volume, containing the shell egg shell egg aqueous solution of yolk antibody: sad: formaldehyde: thimerosal=100:0.3:0.3:0.02; The content of mycillin in this oral liquid is 1000 units per ml;
7) subpackage, preserves.
Embodiment 4:
The formula of anti-house pet viral yolk antibody oral liquid comprises following raw material: containing shell egg aqueous solution 99 L of yolk antibody, sad 0.3 L, formaldehyde 0.2 L, thimerosal 0.01 L, and the mycillin of 1000 units per ml oral liquids.
Its preparation method comprises the following steps:
1) adopt commercially available canine parvovirus to carry out reinforced immunological, every each vaccine consumption of chicken is 2ml, and every minor tick 10 days, carries out three immune programme for children, when antibody titer reaches 1:128, starts to collect high-immunity egg.
2) high-immunity egg will be collected arrange, 0.1% bromo geramine sterilization, for subsequent use.
3) water dilution method acidification: by 2) step high-immunity egg shell egg squeeze into carry out in Pasteur's tank body fully emulsified, emulsification times 9min, then the egg pulp after emulsifying is added the hcl acidifying water that pH is 4.3, temperature is 6 DEG C, hcl acidifying water addition is 5.5 times of egg pulp volume, mixing, place in 5 DEG C of environment and staticly settle for 22 hours, get supernatant; Wherein, hcl acidifying water in water, adds hydrochloric acid make its pH value reach 4.3.
4) sad process; By the supernatant of step 3), the shell egg aqueous solution namely containing yolk antibody weighs, and be warmed up to 28 DEG C, it is sad to add, and fully mixes 2h, then leaves standstill 12h; Wherein, by volume, the supernatant: sad=99:0.3;
5) step 4) gained clear liquor is used successively to the membrane filtration of 1.0mm, 0.45mm, 0.22mm, leave and take filtrate;
6) to step 5) gained filtrate adds formaldehyde, thimerosal, mycillin; By volume, containing the shell egg shell egg aqueous solution of yolk antibody: sad: formaldehyde: thimerosal=99:0.3:0.2:0.01; The content of mycillin in this oral liquid is 1000 units per ml;
7) subpackage, preserves.
With 30 age in days dog 30, first group 10 feeding embodiment 3 products, second group 10 feeding embodiment 4 products, 3rd group 10 only as positive controls, isolated rearing, intramuscular inoculation canine parvovirus dilution virus liquid, when showing clinical symptoms, start treatment, one group, two groups every draught animals take embodiment product 5ml, the 3rd group of every oral normal saline 5ml, every day 1 time, continuous 3 days, every day 1 time, continuous 3 days.72h after treatment, records each group of morbidity and treatment situation.Table 3.
Table 3 experimental conditions
Product of the present invention also can be used for the house pet such as cat, rabbit.
Embodiment 5:
The formula of anti-house pet viral yolk antibody oral liquid comprises following raw material: containing shell egg aqueous solution 103 L of yolk antibody, sad 0.2 L, formaldehyde 0.3 L, thimerosal 0.01 L, and the mycillin of 800 units per ml oral liquids.
Its preparation method comprises the following steps:
1) adopt commercially available canine parvovirus to carry out reinforced immunological, every each vaccine consumption of chicken is 2ml, and every minor tick 10 days, carries out three immune programme for children, when antibody titer reaches 1:256, starts to collect high-immunity egg.
2) high-immunity egg will be collected arrange, 0.2% bromo geramine sterilization, for subsequent use.
3) water dilution method acidification: by 2) step high-immunity egg shell egg squeeze into carry out in Pasteur's tank body fully emulsified, emulsification times 10min, then the egg pulp after emulsifying is added the hcl acidifying water that pH is 4.2, temperature is 4 DEG C, hcl acidifying water addition is 6 times of egg pulp volume, mixing, place in 5 DEG C of environment and staticly settle for 24 hours, get supernatant; Hcl acidifying water in water, adds hydrochloric acid make its pH value reach 4.2.
4) sad process; By the supernatant of step 3), the shell egg aqueous solution namely containing yolk antibody weighs, and be warmed up to 30 DEG C, it is sad to add, and fully mixes 2h, then leaves standstill 12h; Wherein, by volume, the supernatant: sad=103:0.2;
5) step 4) gained clear liquor is used successively to the membrane filtration of 1.0mm, 0.45mm, 0.22mm, leave and take filtrate;
6) to step 5) gained filtrate adds formaldehyde, thimerosal, mycillin; By volume, containing the shell egg shell egg aqueous solution of yolk antibody: sad: formaldehyde: thimerosal=103:0.2:0.3:0.01; The content of mycillin in this oral liquid is 800 units per ml;
7) subpackage, preserves.
Claims (3)
1. the viral yolk antibody oral liquid of anti-house pet, is characterized in that: comprise the following raw material calculated according to volume ratio: containing the shell egg aqueous solution 95-105 of yolk antibody, sad 0.2-0.3, formaldehyde 0.2-0.3, thimerosal 0.01-0.02; Also comprise the mycillin of 800-1000 units per ml oral liquid.
2. a preparation method for the viral yolk antibody oral liquid of anti-house pet as claimed in claim 1, is characterized in that concrete steps are:
1) the viral inactivated vaccine of house pet is adopted to carry out reinforced immunological to chicken: adopt three immune programme for children, every minor tick 10 days, every each vaccine consumption of chicken is 2ml, when antibody titer reaches 1:(128-256) time, carry out high-immunity egg collection;
2) 0.1-0.2% bromo geramine is used to carry out disinfection to high-immunity egg;
3) by 2) in the egg pulp of high-immunity egg carry out emulsifying, emulsification times 7-10min, then add pH be 4.2-4.3, temperature is the hcl acidifying water of 4-6 DEG C, the addition of hcl acidifying water is 5.5-6.5 times of egg pulp volume, after mixing, be cooled to 3-5 DEG C, leave standstill 18-24 hour;
4) get 3) in the supernatant, be warmed up to 25-32 DEG C, it is sad to add, stir 1-2h, leave standstill 10-12h, filter; Wherein sad volume: supernatant volume is (0.2-0.3): (95-105);
5) formaldehyde, thimerosal, mycillin is added, make the shell egg aqueous solution of yolk antibody: sad: formaldehyde: the volume ratio of thimerosal is (95-105): (0.2-0.3): (0.2-0.3): (0.01-0.02), and the content of mycillin is 800-1000 units per ml oral liquid;
6) subpackage, preserves.
3. the preparation method of the viral yolk antibody oral liquid of anti-house pet according to claim 2, is characterized in that: filtered by the filter membrane of 1.0mm, 0.45mm, 0.22mm successively when step 4) is filtered.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109111518A (en) * | 2018-08-31 | 2019-01-01 | 广西壮族自治区兽医研究所 | The special yolk antibody and preparation method thereof for preventing and treating cat distemper heat |
-
2014
- 2014-12-14 CN CN201410762706.1A patent/CN104491860A/en active Pending
Non-Patent Citations (1)
| Title |
|---|
| 张小莺 等: "第五章 卵黄抗体的提取与分离纯化方法", 《卵黄抗体技术》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109111518A (en) * | 2018-08-31 | 2019-01-01 | 广西壮族自治区兽医研究所 | The special yolk antibody and preparation method thereof for preventing and treating cat distemper heat |
| CN109111518B (en) * | 2018-08-31 | 2022-03-15 | 广西壮族自治区兽医研究所 | Specific egg yolk antibody for preventing and treating feline distemper and preparation method thereof |
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