CN1760214A - Method for preparing transparent zinc hyaluronic acid - Google Patents
Method for preparing transparent zinc hyaluronic acid Download PDFInfo
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- CN1760214A CN1760214A CNA2005101176011A CN200510117601A CN1760214A CN 1760214 A CN1760214 A CN 1760214A CN A2005101176011 A CNA2005101176011 A CN A2005101176011A CN 200510117601 A CN200510117601 A CN 200510117601A CN 1760214 A CN1760214 A CN 1760214A
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- zinc
- hyaluronate
- alcoholic solution
- alcohol
- salt
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Abstract
A process for preparing the zinc hyaluronate features the ionic exchange between sodium hyaluronate and Zn salt in alcohol solution.
Description
Technical field
The invention belongs to the bio-pharmaceutical field, relate to a kind of preparation method of zinc hyaluronate.
Background technology
Zinc hyaluronate is hyaluronic zinc salt, the polysaccharide that hyaluronic acid (hyaluronic acid) is made up of the disaccharide repeating unit of glucuronic acid and glucosamine, be present in many reticular tissue such as skin, vitreum, umbilical cord, cartilage, knuckle synovia, and play therein preserve moisture, physiological actions such as nutrition, reparation and pre-antisitic defect, the premium properties that zinc hyaluronate has hyaluronate sodium concurrently also has the antiulcer effect of anti-inflammatory outward.
Hungary has relevant patent (patent publication No. is 1086422) to report the Powdered zinc hyaluronate preparation technology of HA-Zn, is with the hyaluronate sodium dissolving, uses organic solvent deposit behind the adding zinc salt, dehydrates afterwards; The at present domestic method of generally using is a spent ion exchange resin, earlier with the hyaluronic acid dissolving, and last ion exchange column, alcohol precipitation dehydrates again, and both common ground are need be converted under the solution state to carry out, and the production cycle is long, complex process, yield is low.Technology of the present invention by contrast is simple and direct, and it is few to take equipment, and production cost is low, and the yield height has very big superiority.
As bio-pharmaceutical, zinc hyaluronate has excellent biological compatibility, being subjected to the medical circle intensive pays close attention to, narrated the effect of zinc hyaluronate in the Chinese patent 98804672.5 to the treatment peptide ulceration, can treat stomach ulcer, duodenal ulcer and other digestive tract ulcers, have obvious superiority.Because of having stickiness, can form protective membrane on the wound surface, the wound repairing tissue promotes wound healing.To stomatocace, available film is attached on the surface of a wound of oral cavity, has the function that absorbs chronic ulcer tissue secretory product and wound repairing tissue concurrently.
The invention provides a kind of preparation method of Powdered zinc hyaluronate, in alcohol-water solution, can utilize the excess zinc ion that hyaluronate sodium is replaced into zinc hyaluronate under solid state.
Summary of the invention
The invention provides a kind of preparation method of Powdered zinc hyaluronate, in alcohol-water solution, utilize the excess zinc ion that hyaluronate sodium is replaced into zinc hyaluronate under solid state.
Organic solvent of the present invention comprises methyl alcohol and ethanol.
Zinc salt of the present invention, for zinc chloride, zinc sulfate, zinc acetate, zinc nitrate etc. can be dissolved in the inorganic or organic zinc salt of small molecules of alcohol-water solution, wide material sources are easy to get.
Its concentration of alcohol-water solution of the present invention is 40%～100% (volume fraction)
The present invention is the solid powdery product, zinc content 3%～10%.
The preparation technology of this invention zinc hyaluronate is: A. is soaked into hyaluronate sodium in the alcoholic solution of zinc salt, stirs to be beneficial to the speeding-up ion exchange, filters, and removes alcoholic solution.B. repeat A step 2～10 time, obtain the wet solid of zinc hyaluronate.C. soak the wet solid of washing zinc hyaluronate 2～10 times with the alcoholic solution that does not conform to zinc salt.D. the dry zinc hyaluronate that gets.
The specific embodiment of the invention:
Following examples are for illustrating in greater detail the present invention, are not restriction the present invention.
Embodiment 1
With hyaluronate sodium 200 grams, join in 2 liters of spirituous solutions that contain 2% zinc acetate (alcoholic strength is 45%), stirred 6 hours, leave standstill, the alcohol supernatant liquor inclines, replace altogether 6 times, use 95% ethanol dehydration afterwards 5 times, to alcoholic strength 93%, centrifugal, remove ethanol, put the vacuum drying oven drying, to moisture 7%.
Detected result: viscosity: 21mm
2/ s, glucuronic acid content: 42.3%, zinc content 8.9%, sodium content: 0.2%.
Embodiment 2
With hyaluronate sodium 190 grams, stir, add 2 liters of 95% methyl alcohol that contain 3% zinc sulfate, open stirring, after 8 hours, stop to stir, the methyl alcohol supernatant liquor inclines, repeat above step, replace altogether 8 times, use the analytical pure methanol dehydration afterwards 3 times, centrifugal, remove methyl alcohol, put the vacuum drying oven drying, to moisture 7%.
Detected result: viscosity: 19mm
2/ s, glucuronic acid content: 43.1%, zinc content 6.2%, sodium content: 2.7%.
Embodiment 3
With hyaluronate sodium 150 gram, join in 2 liters of ethanolic solns that contain 5% zinc chloride 95%, stirred 10 hours, leave standstill, the alcohol supernatant liquor that inclines repeats above step, replaces altogether 8 times, uses the analytical pure ethanol dehydration afterwards 5 times, centrifugal, remove alcohol, put the vacuum drying oven drying, to moisture 7%.
Detected result: viscosity: 23mm
2/ s, glucuronic acid content: 41.9%, zinc content 9.1%, sodium content: 0.2%.
Claims (6)
- The preparation method of 1 one kinds of zinc hyaluronates.
- Zinc hyaluronate described in 2 claims 1 is called glass acid zinc again.
- The preparation method of the transparent relieving haperacidity zinc described in 3 claims 1, it is characterized in that with the hyaluronate sodium pressed powder in the alcoholic solution of zinc salt by the method for ion-exchange, directly change into zinc hyaluronate.
- Zinc salt described in 4 claims 3, for zinc chloride, zinc sulfate, zinc acetate, zinc nitrate etc. can be dissolved in the inorganic or organic zinc salt of small molecules of alcoholic solution, its concentration in alcoholic solution is 1%～15%.
- Alcohol is methyl alcohol or ethanol described in 5 claims 3 or 4, and its strength of solution is 40%～100% (volume fraction).
- 6 this invent and be the solid powdery product, zinc content is 3%～10%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005101176011A CN100355790C (en) | 2005-11-04 | 2005-11-04 | Method for preparing transparent zinc hyaluronic acid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005101176011A CN100355790C (en) | 2005-11-04 | 2005-11-04 | Method for preparing transparent zinc hyaluronic acid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1760214A true CN1760214A (en) | 2006-04-19 |
| CN100355790C CN100355790C (en) | 2007-12-19 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNB2005101176011A Active CN100355790C (en) | 2005-11-04 | 2005-11-04 | Method for preparing transparent zinc hyaluronic acid |
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| CN (1) | CN100355790C (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011129370A1 (en) * | 2010-04-14 | 2011-10-20 | キユーピー株式会社 | Process for producing hyaluronic acid metal salt, process for producing cosmetic containing hyaluronic acid metal salt, and zinc hyaluronate and process for producing same |
| CN102552309A (en) * | 2010-12-15 | 2012-07-11 | 北京理工大学 | Application and preparation method of gold hyaluronic acid |
| CN102584657A (en) * | 2011-12-27 | 2012-07-18 | 杭州海虹精细化工有限公司 | Preparation method of persulfide/sulfide zinc acetate |
| CN111647100A (en) * | 2020-07-14 | 2020-09-11 | 山东华熙海御生物医药有限公司 | Preparation method of high molecular weight zinc hyaluronate |
| WO2023202613A1 (en) * | 2022-04-20 | 2023-10-26 | 华熙生物科技股份有限公司 | Preparation method and application of zinc hyaluronate |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| HUP0900717A3 (en) * | 2009-11-18 | 2012-02-28 | Richter Gedeon Nyrt | Pharmaceutical composition for urological use containing zinc hyaluronate |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| HU225991B1 (en) * | 1997-04-29 | 2008-02-28 | Richter Gedeon Nyrt | Use of the zinc hyaluronate associate (complex) for the preparation of pharmaceutical compositions for oral use against peptic ulcer |
| JP3779200B2 (en) * | 2001-11-28 | 2006-05-24 | 電気化学工業株式会社 | Stabilized composition of aqueous solution of hyaluronic acid and / or salt thereof |
| CZ12015U1 (en) * | 2002-01-18 | 2002-02-25 | Cpn Spol. S R.O. | Preparation for preventing adhesion of a bandage to a wound |
| CZ13928U1 (en) * | 2003-10-08 | 2004-01-20 | Cpn Spol. S R.O. | Dietetic preparation for the prevention and treatment of osteoporosis |
| CN1634118A (en) * | 2004-11-05 | 2005-07-06 | 凌沛学 | Antivirus formulation containing polysaccharide polyanion zinc salt and its preparing process |
-
2005
- 2005-11-04 CN CNB2005101176011A patent/CN100355790C/en active Active
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011129370A1 (en) * | 2010-04-14 | 2011-10-20 | キユーピー株式会社 | Process for producing hyaluronic acid metal salt, process for producing cosmetic containing hyaluronic acid metal salt, and zinc hyaluronate and process for producing same |
| CN102834417A (en) * | 2010-04-14 | 2012-12-19 | 丘比株式会社 | Process for producing hyaluronic acid metal salt, process for producing cosmetic containing hyaluronic acid metal salt, and zinc hyaluronate and process for producing same |
| CN102834417B (en) * | 2010-04-14 | 2015-11-25 | 丘比株式会社 | The manufacture method of hyaluronic acid metal salt, the manufacture method comprising the makeup of hyaluronic acid metal salt and zinc hyaluronate and manufacture method thereof |
| JP5852560B2 (en) * | 2010-04-14 | 2016-02-03 | キユーピー株式会社 | Method for producing metal hyaluronate, method for producing cosmetics containing metal hyaluronate, zinc hyaluronate and method for producing the same |
| CN102552309A (en) * | 2010-12-15 | 2012-07-11 | 北京理工大学 | Application and preparation method of gold hyaluronic acid |
| CN102552309B (en) * | 2010-12-15 | 2013-06-05 | 北京理工大学 | Application and preparation method of gold hyaluronic acid |
| CN102584657A (en) * | 2011-12-27 | 2012-07-18 | 杭州海虹精细化工有限公司 | Preparation method of persulfide/sulfide zinc acetate |
| CN111647100A (en) * | 2020-07-14 | 2020-09-11 | 山东华熙海御生物医药有限公司 | Preparation method of high molecular weight zinc hyaluronate |
| WO2023202613A1 (en) * | 2022-04-20 | 2023-10-26 | 华熙生物科技股份有限公司 | Preparation method and application of zinc hyaluronate |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100355790C (en) | 2007-12-19 |
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Address after: 250014 No. 264 mountain road, Lixia District, Shandong, Ji'nan Patentee after: Shandong Freda Biopharm Co., Ltd. Address before: 250014 No. 264 mountain road, Lixia District, Shandong, Ji'nan Patentee before: Furuida Biological Chemical Co., Ltd., Shandong |
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Address after: Tianchen Avenue, Ji'nan hi tech Development Zone of Shandong Province, No. 678 250101 Patentee after: Bloomage Freda Biopharm Co., Ltd. Address before: 250014 No. 264 mountain road, Lixia District, Shandong, Ji'nan Patentee before: Shandong Freda Biopharm Co., Ltd. |
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Address after: 250101 678 Tianchen street, hi tech Development Zone, Ji'nan, Shandong Patentee after: Huaxi Biotechnology Co., Ltd. Address before: 250101 678 Tianchen street, hi tech Development Zone, Ji'nan, Shandong Patentee before: Bloomage Freda Biopharm Co., Ltd. |