CN1746193A - Preparation of low-molecular weight chitoglycan or chitooligose - Google Patents
Preparation of low-molecular weight chitoglycan or chitooligose Download PDFInfo
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- CN1746193A CN1746193A CN 200510019438 CN200510019438A CN1746193A CN 1746193 A CN1746193 A CN 1746193A CN 200510019438 CN200510019438 CN 200510019438 CN 200510019438 A CN200510019438 A CN 200510019438A CN 1746193 A CN1746193 A CN 1746193A
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Abstract
Production of low-molecular weight chitoglycan or chitooligose is carried out by fixing dispase on N-succinchitoglycan aquogel ball from cross-linking method, degrading chitoglycan from fixed enzyme, controlling enzymolysis time, regulating degraded product pH to 8-9 from sodium hydroxide, depositing by alcohol, washing and vacuum drying to obtain low-molecular weight chitoglycan or chitooligose with free amidogen. It achieves much yield, less molecular weight distribution and continuous industrial production. It can be used for medicine and food.
Description
Technical field
The present invention relates to the preparation method of a kind of low-molecular weight chitoglycan or oligochitosan, belong to renewable resource chemicobiology field.
Background technology
Low-molecular weight chitoglycan and oligochitosan are the degraded products behind the de-acetyl chitin, have than the better solvability of macromolecule chitosan, biocompatibility.The chitosan of different molecular weight has different bio-physiological activities, so be applied to aspects such as food, medicine for chitosan significant for the chitosan of preparation different molecular weight.The method for preparing at present low-molecular weight chitoglycan and oligochitosan mainly contains chemical method and biological process, and the chemical method reaction process is violent, is not easy to obtain low-molecular weight chitoglycan and product structure and is modified.Enzymic degradation just interrupts β (1-4) glycoside bond of chitosan, generally do not change the residue structure of chitosan molecule, and the specificity enzyme costs an arm and a leg, be difficult for a large amount of the acquisition, is not suitable for the suitability for industrialized production of low-molecular weight chitoglycan and oligochitosan; Use free enzymic hydrolysis to prepare low-molecular weight chitoglycan and oligochitosan, enzyme can not be reused after reaction, uneconomical, severe reaction conditions, product contains the mixture of 0.1% (w/w) sugar and zymoprotein, and this product can not be applied to food, medicine and other fields owing to can cause pyrogen reaction.
Summary of the invention
At above-mentioned problems of the prior art, the invention provides and a kind ofly can prepare the low-molecular weight chitoglycan that contains the zymoprotein mixture hardly or the method for oligochitosan continuously.It is non-specificity enzyme that this method is utilized neutral protease, effective degrade chitosan, can suitability for industrialized production, inexpensive, the advantage of stay in grade, neutral protease is fixed on the N-succinyl-chitosan hydrogel sphere with crosslinking, improved enzyme activity, enlarged the stability of enzyme, avoided the generation of degraded product and zymoprotein mixture temperature and pH, improve the product productive rate, reduced the molecular weight distribution of product.
Technical scheme provided by the invention is: at first use N-succinyl-chitosan hydrogel sphere as the immobilized carrier of neutral protease when preparation low-molecular weight chitoglycan or oligochitosan, make linking agent with glutaraldehyde, make the neutral protease that is fixed on the N-succinyl-chitosan hydrogel sphere, then with the immobilized enzyme hydrolysis chitosan that makes, control the enzyme digestion reaction time as required, can obtain required low-molecular weight chitoglycan or oligochitosan.
The low-molecular weight chitoglycan of no zymoprotein mixture or oligochitosan preparation method's concrete steps are as follows:
(1) the N-succinyl-chitosan is soluble in water, making concentration is the N-succinyl-chitosan aqueous solution of 0.25-0.40g/ml, calcium chloride solution and the dehydrated alcohol under magnetic agitation this solution dropwise clamp-oned by 0.1-0.3g/ml mixed acquisition N-succinyl-chitosan hydrogel sphere in the solidification liquid that forms in 3: 7～7: 3 with volume ratio, N-succinyl-chitosan hydrogel sphere was kept 2-6 hour being fixed carrier N-succinyl-chitosan hydrogel sphere in the solidification liquid system.
(2) remove by filter solidification liquid, N-succinyl-chitosan hydrogel sphere is placed the 0.05-0.5M citric acid-Sodium phosphate dibasic that is dissolved with glutaraldehyde, the pH value is in the buffered soln of 3.0-5.0, the concentration of volume percent of glutaraldehyde is 0.5-1.5% in the solution, the quality of N-succinyl-chitosan hydrogel sphere and linking agent glutaraldehyde (weight in wet base) volume ratio is 1: 3～1: 10, under the room temperature continuous oscillation 4-12 hour, filter then and collect crosslinked N-succinyl-chitosan hydrogel sphere, water or 0.05-0.5M citric acid-Sodium phosphate dibasic damping fluid repetitive scrubbing, do not have absorption at the 245nm place until washings, obtain crosslinked fixation support.
(3) will be that the neutral protease of 0.2-1.5 is dissolved in 0.05-0.5M citric acid-Sodium phosphate dibasic, pH is in the buffered soln of 3.0-5.0 with the fixation support weight ratio, in this enzyme solution, add crosslinked fixation support, room temperature continuous oscillation 2-6 hour, remove by filter enzyme solution, be to wash immobilized enzyme in the buffered soln of 3.0-5.0 with 0.05-0.5M citric acid-Sodium phosphate dibasic, pH again, do not have absorption at the 280nm place until washings, obtain to be fixed on the neutral protease on the N-succinyl-chitosan hydrogel sphere.
(4) the deacetylation scope being dissolved in concentration of volume percent at the chitosan of 75%-95% is in the 0.5-1.5% acetum, adjust the pH value of solution value to 5.0-6.0 with the sodium hydroxide solution of 0.05-1mol/l, add the neutral protease that is fixed on the N-succinyl-chitosan hydrogel sphere then, the enzyme that adds is the 3%-10% of chitosan mass, at 45-55 ℃ of continuous oscillation required time, filter and collect degraded product, obtain low-molecular weight chitoglycan or oligochitosan.
Will be behind the degraded product vacuum concentration transfer pH 8-9 with the sodium hydroxide of 1-5mol/l, with ethanol sedimentation, washing, vacuum-drying can obtain weight-average molecular weight at 2.35 ten thousand～1900 free amino group form low-molecular weight chitoglycan and oligochitosan.When the enzymatic hydrolysis reaction times is between 0.5-5 hour the time, obtains low-molecular weight chitoglycan, when the enzymatic hydrolysis reaction times more than or equal to 5 hours, obtain oligochitosan.
Wash in the buffered soln of 3.0-5.0 if with 0.2M citric acid-Sodium phosphate dibasic, pH value be used immobilized enzyme, can remove the degraded product that adsorbs on the immobilized enzyme, immobilized enzyme is reused, if add new chitosan solution again, just can realize that continuous preparation does not contain the low-molecular weight chitoglycan and the oligochitosan of zymoprotein mixture.
The molecular weight ranges of used chitosan is preferably 12.8 ten thousand-64.8 ten thousand in this method.
The N-succinyl-chitosan is the derivative of chitosan, can be by chitosan and Succinic anhydried prepared in reaction (preparation method of N-succinyl-chitosan can prepare with reference to relevant document).Because Succinic anhydried has been introduced carboxyl when replacing a part of amino of chitosan, stoped the amino of chitosan and hydroxyl to form hydrogen bond, thereby improved water-soluble.Simultaneously, the N-succinyl-chitosan has replaced a part of amino of chitosan, has reduced the consumption of linking agent glutaraldehyde, has kept the higher enzyme of immobilized enzyme to live.In addition, on fixed enzyme vector, introduce carboxyl, increased the avidity of carrier and enzyme, improved the immobilization rate of enzyme.So N-succinyl-chitosan hydrogel sphere is suitable as the fixation support of enzyme.N-succinyl-chitosan hydrogel sphere provided by the invention has mechanical property preferably, and the loss of living of this immobilized enzyme enzyme in use repeatedly is less, and the continuous degradation chitosan still kept 82.5% of its initial vigor in 40 hours.Use the immobilized enzyme degrade chitosan, the mixture that contains sugar/zymoprotein in the hydrolysate hardly, so the low-molecular weight chitoglycan and the oligochitosan that make of method can be owing to the compound zymoprotein produce pyrogen reaction, so be particularly useful for fields such as medicine, food thus.
Embodiment
Preparation provided by the invention there is not the low-molecular weight chitoglycan of zymoprotein mixture or the method for oligochitosan elaborates below by specific embodiment.
Embodiment 1: the N-succinyl-chitosan is made the 0.25g/ml aqueous solution, under magnetic agitation, dropwise clamp-on in the mixed solution of 30ml 0.3g/ml calcium chloride and the formation of 70ml dehydrated alcohol with syringe (3# syringe needle), room temperature continues to stir 6 hours, obtains N-succinyl-chitosan hydrogel sphere.Take by weighing this hydrogel sphere 4g (weight in wet base), place 12ml to be dissolved with in citric acid-disodium hydrogen phosphate buffer solution that volume percent is 0.5% glutaraldehyde (0.05M, pH 3.0), room temperature vibration 12 hours.Filter and collect crosslinked N-succinyl-chitosan hydrogel sphere, the water repetitive scrubbing does not have absorption until washings at the 245nm place.To be dissolved in 0.05M citric acid-disodium hydrogen phosphate buffer solution in (pH 3.0) than the neutral protease that is 0.2 with fixation support weight (weight in wet base), in this enzyme solution, add crosslinked fixation support, room temperature continuous oscillation 6 hours.Then, remove by filter enzyme solution,, do not have absorption at the 280nm place, obtain to be fixed on the neutral protease on the N-succinyl-chitosan hydrogel sphere until washings with (pH 3.0) washing immobilized enzyme in 0.05M citric acid-disodium hydrogen phosphate buffer solution.With 0.1g chitosan (weight-average molecular weight 64.8 ten thousand, deacetylation 75%) is dissolved in 10ml 0.5% (v/v) acetum, sodium hydroxide solution with 0.05mol/l is adjusted pH value of solution value to 5.0, add zymoprotein and chitosan mass than the immobilized enzyme that is 3%, 45 ℃ of continuous oscillations 0.5 hour, filter and collect degraded product, sodium hydroxide with 1mol/l behind the vacuum concentration is transferred pH 8-9, ethanol sedimentation, washing with alcohol, it is 2.35 ten thousand that vacuum-drying obtains weight-average molecular weight, molecular weight distribution 5.31, the free amino group form low-molecular weight chitoglycan of productive rate 91.4%.
Embodiment 2: the N-succinyl-chitosan is made the 0.30g/ml aqueous solution, under magnetic agitation, dropwise clamp-on in the mixed solution of 40ml 0.15g/ml calcium chloride and the formation of 60ml dehydrated alcohol with syringe (4# syringe needle), room temperature continues to stir 3 hours, obtains N-succinyl-chitosan hydrogel sphere.Take by weighing this hydrogel sphere 4g (weight in wet base), place 16ml to be dissolved with in citric acid-disodium hydrogen phosphate buffer solution that volume percent is 0.8% glutaraldehyde (0.2M, pH 3.5), room temperature vibration 10 hours.Filter and collect crosslinked N-succinyl-chitosan hydrogel sphere, the water repetitive scrubbing does not have absorption until washings at the 245nm place.To be dissolved in 0.2M citric acid-disodium hydrogen phosphate buffer solution in (pH 3.5) than the neutral protease that is 0.45 with fixation support weight (weight in wet base), in this enzyme solution, add crosslinked fixation support, room temperature continuous oscillation 3 hours.Then, remove by filter enzyme solution,, do not have absorption at the 280nm place, obtain to be fixed on the neutral protease on the N-succinyl-chitosan hydrogel sphere until washings with (pH 3.5) washing immobilized enzyme in 0.2M citric acid-disodium hydrogen phosphate buffer solution.With 0.1g chitosan (weight-average molecular weight 38.6 ten thousand, deacetylation 86%) is dissolved in 10ml 0.8% (v/v) acetum, sodium hydroxide solution with 0.1mol/l is adjusted pH value of solution value to 5.4, add zymoprotein and chitosan mass than the immobilized enzyme that is 5%, 50 ℃ of continuous oscillations 1 hour, filter and collect degraded product, sodium hydroxide with 2mol/l behind the vacuum concentration is transferred pH 8-9, ethanol sedimentation, washing with alcohol, it is 1.58 ten thousand that vacuum-drying obtains weight-average molecular weight, molecular weight distribution 4.77, the free amino group form low-molecular weight chitoglycan of productive rate 87.9%.
Embodiment 3: the N-succinyl-chitosan is made the 0.30g/ml aqueous solution, under magnetic agitation, dropwise clamp-on in the mixed solution of 50ml 0.2g/ml calcium chloride and the formation of 50ml dehydrated alcohol with syringe (5# syringe needle), room temperature continues to stir 4 hours, obtains N-succinyl-chitosan hydrogel sphere.Take by weighing this hydrogel sphere 4g (weight in wet base), place 20ml to be dissolved with in citric acid-disodium hydrogen phosphate buffer solution that volume percent is 1.0% glutaraldehyde (0.3M, pH 4.0), room temperature vibration 8 hours.Filter and collect crosslinked N-succinyl-chitosan hydrogel sphere,, do not have absorption at the 245nm place until washings with 0.3M citric acid-disodium hydrogen phosphate buffer solution (pH 4.0) repetitive scrubbing.To be dissolved in 0.3M citric acid-disodium hydrogen phosphate buffer solution in (pH 4.0) than the neutral protease that is 0.6 with fixation support weight (weight in wet base), in this enzyme solution, add crosslinked fixation support, room temperature continuous oscillation 4 hours.Then, remove by filter enzyme solution,, do not have absorption at the 280nm place, obtain to be fixed on the neutral protease on the N-succinyl-chitosan hydrogel sphere until washings with (pH 4.0) washing immobilized enzyme in 0.3M citric acid-disodium hydrogen phosphate buffer solution.With 0.1g chitosan (weight-average molecular weight 26.8 ten thousand, deacetylation 92%) is dissolved in 10ml 1.0% (v/v) acetum, sodium hydroxide solution with 0.5mol/l is adjusted pH value of solution value to 5.7, add zymoprotein and chitosan mass than the immobilized enzyme that is 5%, 55 ℃ of continuous oscillations 2 hours, filter and collect degraded product, sodium hydroxide with 3mol/l behind the vacuum concentration is transferred pH 8-9, ethanol sedimentation, washing with alcohol, it is 1.08 ten thousand that vacuum-drying obtains weight-average molecular weight, molecular weight distribution 4.18, the free amino group form low-molecular weight chitoglycan of productive rate 81.3%.
Embodiment 4: the N-succinyl-chitosan is made the 0.40g/ml aqueous solution, under magnetic agitation, dropwise clamp-on in the mixed solution of 60ml 0.3g/ml calcium chloride and the formation of 40ml dehydrated alcohol with syringe (6# syringe needle), room temperature continues to stir 6 hours, obtains N-succinyl-chitosan hydrogel sphere.Take by weighing this hydrogel sphere 4g (weight in wet base), place 30ml to be dissolved with in citric acid-disodium hydrogen phosphate buffer solution that volume percent is 1.2% glutaraldehyde (0.45M, pH 4.5), room temperature vibration 6 hours.Filter and collect crosslinked N-succinyl-chitosan hydrogel sphere,, do not have absorption at the 245nm place until washings with 0.45M citric acid-disodium hydrogen phosphate buffer solution (pH 4.5) repetitive scrubbing.To be dissolved in 0.45M citric acid-disodium hydrogen phosphate buffer solution in (pH 4.5) than the neutral protease that is 1.2 with fixation support weight (weight in wet base), in this enzyme solution, add crosslinked fixation support, room temperature continuous oscillation 4 hours.Then, remove by filter enzyme solution,, do not have absorption at the 280nm place, obtain to be fixed on the neutral protease on the N-succinyl-chitosan hydrogel sphere until washings with (pH 4.5) washing immobilized enzyme in 0.45M citric acid-disodium hydrogen phosphate buffer solution.With 0.1g chitosan (weight-average molecular weight 12.8 ten thousand, deacetylation 95%) is dissolved in 10ml 1.0% (v/v) acetum, sodium hydroxide solution with 0.8mol/l is adjusted pH value of solution value to 5.4, add zymoprotein and chitosan mass than the immobilized enzyme that is 8%, 48 ℃ of continuous oscillations 4 hours, filter and collect degraded product, sodium hydroxide with 4mol/l behind the vacuum concentration is transferred pH 8-9, ethanol sedimentation, washing with alcohol, it is 4500 that vacuum-drying obtains weight-average molecular weight, molecular weight distribution 3.32, the free amino group form low-molecular weight chitoglycan of productive rate 72.4%.
Embodiment 5: the N-succinyl-chitosan is made the 0.40g/ml aqueous solution, under magnetic agitation, dropwise clamp-on in the mixed solution of 70ml 0.1g/ml calcium chloride and the formation of 30ml dehydrated alcohol with syringe (7# syringe needle), room temperature continues to stir 2 hours, obtains N-succinyl-chitosan hydrogel sphere.Take by weighing this hydrogel sphere 4g (weight in wet base), place 40ml to be dissolved with in citric acid-disodium hydrogen phosphate buffer solution that volume percent is 1.5% glutaraldehyde (0.5M, pH 5.0), room temperature vibration 4 hours.Filter and collect crosslinked N-succinyl-chitosan hydrogel sphere,, absorb in 245nm place nothing until washings with 0.5M citric acid-disodium hydrogen phosphate buffer solution (pH 5.0) repetitive scrubbing.To be dissolved in 0.5M citric acid-disodium hydrogen phosphate buffer solution in (pH 5.0) than the neutral protease that is 1.5 with fixation support weight (weight in wet base), in this enzyme solution, add crosslinked fixation support, room temperature continuous oscillation 2 hours.Then, remove by filter enzyme solution,, do not have absorption at the 280nm place, obtain to be fixed on the neutral protease on the N-succinyl-chitosan hydrogel sphere until washings with (pH 5.0) washing immobilized enzyme in 0.5M citric acid-disodium hydrogen phosphate buffer solution.With 0.1g chitosan (weight-average molecular weight 50.8 ten thousand, deacetylation 81%) is dissolved in 10ml 1.5% (v/v) acetum, sodium hydroxide solution with 1mol/l is adjusted pH value of solution value to 6.0, add zymoprotein and chitosan mass than the immobilized enzyme that is 10%, 50 ℃ of continuous oscillations 5 hours, filter and collect degraded product, sodium hydroxide with 5mol/l behind the vacuum concentration is transferred pH 8-9, ethanol sedimentation, washing with alcohol, it is 1900 that vacuum-drying obtains weight-average molecular weight, molecular weight distribution 1.28, the free amino group form oligochitosan of productive rate 61.4%.
Claims (6)
1, the preparation method of a kind of low-molecular weight chitoglycan or oligochitosan, it is characterized in that: at first use N-succinyl-chitosan hydrogel sphere as the immobilized carrier of neutral protease when preparation low-molecular weight chitoglycan or oligochitosan, make linking agent with glutaraldehyde, make the neutral protease that is fixed on the N-succinyl-chitosan hydrogel sphere, then with the immobilized enzyme hydrolysis chitosan that makes, control the enzyme digestion reaction time as required, can obtain required low-molecular weight chitoglycan or oligochitosan.
2, the preparation method of low-molecular weight chitoglycan according to claim 1 or oligochitosan is characterized in that the concrete steps that adopt are as follows:
(1) the N-succinyl-chitosan is soluble in water, making concentration is the N-succinyl-chitosan aqueous solution of 0.25-0.40g/ml, calcium chloride solution and the dehydrated alcohol under magnetic agitation this solution dropwise clamp-oned by 0.1-0.3g/ml mixed acquisition N-succinyl-chitosan hydrogel sphere in the solidification liquid that forms in 3: 7～7: 3 with volume ratio, N-succinyl-chitosan hydrogel sphere was kept 2-6 hour being fixed carrier N-succinyl-chitosan hydrogel sphere in the solidification liquid system.
(2) remove by filter solidification liquid, N-succinyl-chitosan hydrogel sphere is placed the 0.05-0.5M citric acid-Sodium phosphate dibasic that is dissolved with glutaraldehyde, the pH value is in the buffered soln of 3.0-5.0, the concentration of volume percent of glutaraldehyde is 0.5-1.5% in the solution, the quality of N-succinyl-chitosan hydrogel sphere and linking agent glutaraldehyde (weight in wet base) volume ratio is 1: 3～1: 10, under the room temperature continuous oscillation 4-12 hour, filter then and collect crosslinked N-succinyl-chitosan hydrogel sphere, water or 0.05-0.5M citric acid-Sodium phosphate dibasic damping fluid repetitive scrubbing, do not have absorption at the 245nm place until washings, obtain crosslinked fixation support.
(3) will be that the neutral protease of 0.2-1.5 is dissolved in 0.05-0.5M citric acid-Sodium phosphate dibasic, pH is in the buffered soln of 3.0-5.0 with the fixation support weight ratio, in this enzyme solution, add crosslinked fixation support, room temperature continuous oscillation 2-6 hour, remove by filter enzyme solution, be to wash immobilized enzyme in the buffered soln of 3.0-5.0 with 0.05-0.5M citric acid-Sodium phosphate dibasic, pH again, do not have absorption at the 280nm place until washings, obtain to be fixed on the neutral protease on the N-succinyl-chitosan hydrogel sphere.
(4) the deacetylation scope being dissolved in concentration of volume percent at the chitosan of 75%-95% is in the 0.5-1.5% acetum, adjust the pH value of solution value to 5.0-6.0 with the sodium hydroxide solution of 0.05-1mol/l, add the neutral protease that is fixed on the N-succinyl-chitosan hydrogel sphere then, the enzyme that adds is the 3%-10% of chitosan mass, at 45-55 ℃ of continuous oscillation required time, filter and collect degraded product, obtain low-molecular weight chitoglycan or oligochitosan.
3, the preparation method of low-molecular weight chitoglycan according to claim 2 or oligochitosan, it is characterized in that: regulate the pH value to 8-9 with the sodium hydroxide of 1-5mol/l after will filtering the degraded product vacuum concentration of collecting, with ethanol sedimentation, washing, vacuum-drying obtains low-molecular weight chitoglycan or the oligochitosan of weight-average molecular weight in 2.35 ten thousand～1900 free amino group form.
4, the preparation method of low-molecular weight chitoglycan according to claim 2 or oligochitosan, it is characterized in that: when the enzymatic hydrolysis reaction times is between 0.5-5 hour the time, obtain low-molecular weight chitoglycan, when the enzymatic hydrolysis reaction times more than or equal to 5 hours, obtain oligochitosan.
5, the preparation method of low-molecular weight chitoglycan according to claim 2 or oligochitosan, it is characterized in that: be the buffered soln washing of 3.0-5.0 with 0.05-0.5M citric acid-Sodium phosphate dibasic, pH with the exhausted immobilized enzyme at last, remove the degraded product that adsorbs on the immobilized enzyme, it can be reused.
6, the preparation method of low-molecular weight chitoglycan according to claim 1 and 2 or oligochitosan is characterized in that: the molecular weight ranges of used chitosan is 12.8 ten thousand-64.8 ten thousand.
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| CN100439496C (en) * | 2006-05-12 | 2008-12-03 | 成都医学院 | Fixed trypsinase and its preparation method |
| CN100584955C (en) * | 2006-12-27 | 2010-01-27 | 江西师范大学 | Chitosan oligosaccharide/chito-oligomer single enzyme production process |
| US7943597B2 (en) | 2008-04-08 | 2011-05-17 | Cypress Pharmaceutical, Inc. | Phosphate-binding chitosan and uses thereof |
| CN102140143A (en) * | 2011-05-09 | 2011-08-03 | 广州优宝生物科技有限公司 | Method for extracting chitin from crab shells |
| CN1975419B (en) * | 2006-12-11 | 2012-05-09 | 武汉大学 | Water soluble chitin pH probe and producing method thereof |
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| CN108003257A (en) * | 2017-10-11 | 2018-05-08 | 中科荣信（苏州）生物科技有限公司 | A kind of chitosan oligosaccharide with specific structure and its preparation method and application |
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| JPH0775546A (en) * | 1993-06-28 | 1995-03-20 | New Japan Radio Co Ltd | Immobilized biologically related substance column |
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| CN100439496C (en) * | 2006-05-12 | 2008-12-03 | 成都医学院 | Fixed trypsinase and its preparation method |
| CN1975419B (en) * | 2006-12-11 | 2012-05-09 | 武汉大学 | Water soluble chitin pH probe and producing method thereof |
| CN100584955C (en) * | 2006-12-27 | 2010-01-27 | 江西师范大学 | Chitosan oligosaccharide/chito-oligomer single enzyme production process |
| US7943597B2 (en) | 2008-04-08 | 2011-05-17 | Cypress Pharmaceutical, Inc. | Phosphate-binding chitosan and uses thereof |
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| CN102140143B (en) * | 2011-05-09 | 2012-11-21 | 广州优宝生物科技有限公司 | Method for extracting chitin from crab shells |
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| CN110628848A (en) * | 2019-10-22 | 2019-12-31 | 江南大学 | Method for efficiently preparing chitosan oligosaccharide with polymerization degree of 2-6 |
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