CN1733070A - Chinese medicinal preparation for treating cardiovascular and cerebrovascular diseases and its preparing process - Google Patents
Chinese medicinal preparation for treating cardiovascular and cerebrovascular diseases and its preparing process Download PDFInfo
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Abstract
The invention relates to a Chinese medicinal preparation for treating cardiovascular and cerebrovascular diseases and its preparing process, wherein the preparation is prepared from red sage root, safflower and astragalus root by the weight ratio of 1:0.1-10:0.1-10.
Description
Technical field: the present invention relates to Chinese medicine preparation for the treatment of cardiovascular and cerebrovascular disease and preparation method thereof, belong to the field of medical technology.
Background technology: along with the development of human civilization, the raising of people's living standard, the cardiovascular and cerebrovascular diseases such as coronary heart diseases and angina pectoris have become human second largest killer. Exploitation heart disease class medicine has become instant challenge of pharmacy industry. Same veriety Main Ingredients and Appearance in the market mostly is the red sage root, the general color and luster of its finished product is darker, and transportation and storage are inconvenient, and oxidation stain easily occurs, the pH value also can change thereupon, is restricted so that contain the term of validity and the storage life of the injection products of the red sage root. CN 1272500A discloses safflower total uranidin and has had antithrombotic formation, suppresses platelet aggregation, resists myocardial ischemia, and can treat or prevent cardiovascular and cerebrovascular disease; CN 1247855A discloses preparation method and the purposes of polydanshinolate, can be used for preparing cardiovascular and cerebrovascular medicine. The applicant had once applied for to Patent Office of the People's Republic of China that name was called: " a kind of pharmaceutical composition for the treatment of cardiovascular and cerebrovascular disease and preparation method thereof ", number of patent application are 02153312.1 patent of invention, and the applicant finds that the water extract combination of the water extract combination of red sage root alcohol extract and safflower or red sage root alcohol extract and the red sage root and safflower is had synergy to the treatment cardiovascular and cerebrovascular; We find in research process: the red sage root, safflower all belong to blood-activating drug, curative effect is desirable not enough, and brings out easily hemorrhagely, is unfavorable for having this part patient of hemorrhagic tendency, and red sage root alcohol extract is water-soluble poor in the preparation method who provides, and preparation stability is not ideal.
Summary of the invention: the object of the invention is to: a kind of Chinese medicine preparation of cardiovascular and cerebrovascular disease and other preparation method for the treatment of is provided, it mainly is to add the medicine that is used for tonifying Qi in the preparation that the red sage root, safflower form again: the Radix Astragali, like this can qi and activate blood circulation, treat various syndrome of blood stasis due to qi deficiency. The applicant is under motherland's medical science qi and blood theoretical direction, and in conjunction with clinical practice, it is unable to think that the deficiency of vital energy is then transported blood, and blood stagnates and the stasis of blood without freely, and blood stasis can increase the weight of again the deficiency of vital energy, so assertive evidence is the card of asthenia in origin and asthenia in superficiality, the deficiency of vital energy is this, and blood stasis is mark. It is main controlling beneficial tonifying Qi, is aided with promoting blood circulation and removing blood stasisly, makes unit be so incensed that multiple, and the blood stasis of blood of living is gone, and qi and blood is transferred smooth; This pharmaceutical preparation that utilizes the inventor to provide, can more effectively treat the cardiovascular and cerebrovascular diseases such as coronary heart diseases and angina pectoris, simple, the successful of the prescription of this preparation of while, the quality of production is beneficial to control, its formulation is many, be convenient to the doctor and the patient selects, satisfied the instructions for use of different crowd, varying environment; The invention provides in addition the preparation method of these preparations, particularly be prepared into the method for various injections, can directly instruct suitability for industrialized production.
The present invention consists of like this: the Chinese medicine preparation for the treatment of cardiovascular and cerebrovascular disease: calculate with part by weight: it is by the red sage root: safflower: the Radix Astragali=1: 0.1～10: 0.1～10 or the preparation made from the extract of the medicinal material of corresponding weight ratio. Specifically: calculate with part by weight: it is by the red sage root: safflower: the Radix Astragali=1: 0.1～1: 0.1～1 or the preparation made from the extract of the medicinal material of corresponding weight ratio.
Say accurately: calculate with part by weight: it is by the red sage root: safflower: the Radix Astragali=1: 0.1～0.5: 0.1～0.8 or the preparation made from the extract of the medicinal material of corresponding weight ratio.
Preferably: calculate with part by weight: it is by the red sage root: safflower: the preparation that the extract of the medicinal material of the Radix Astragali=3: 1: 2 or corresponding weight ratio is made.
Preparation of the present invention is injection, capsule, soft capsule, micro-capsule, granule, pill, dripping pill, sustained release preparation, controlled release preparation, quick releasing formulation, targeting preparation, oral liquid, syrup, powder or mixture.
Described injection comprise the injection with small volume that is directly used in drug administration by injection, directly for the venous transfusion of drip-feed, need to be used for after the dilution concentrated solution for injection and the injection sterile powder of drip-feed.
Its preparation method is: get the red sage root, safflower, the Radix Astragali, pulverize, water or ethanolic solution extract separately, make with extra care respectively, merge extract, are prepared into different preparations.
Specifically: get the red sage root, be ground into meal, add alcohol extract, measuring relative density during extract decompression recycling ethanol to 60 ℃ is 1.05～1.15, adopt again one or more methods in flocculent precipitation, extraction, depositing in water method or the column chromatography to unite to use and make with extra care, get thick paste I; Get safflower, be ground into meal, measuring relative density when extracting in water, extract are evaporated to 60 ℃ is 1.05～1.15, adopt again one or more methods in alcohol deposition method, extraction, flocculent precipitation or the column chromatography to unite to use and make with extra care, get thick paste II; Get the Radix Astragali, be ground into the meal of 10 mesh sieves, add alcohol extract, extract filters, measuring relative density when filtrate is concentrated into 60 ℃ is 1.05～1.15, adopt again one or more methods in depositing in water method, flocculent precipitation, extraction or the column chromatography to unite to use and make with extra care, get thick paste II I; Merge above-mentioned thick paste I, II, III, make different preparations.
More specifically: get the red sage root, be ground into the meal of 10 mesh sieves, adding 6～10 times of volume 50%～90% alcohol refluxs extracts 1～4 time, each 0.5～4 hour, merge extract, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, the water-soluble solution that adds 2～4 times of medicinal material volumes, filter, filtrate is crossed D-101 type macroporous absorbent resin, with 3～10 times of resinite ponding flushing impurity, then use 20%～50% ethanol desorb of 2～8 times of resin volumes, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, gets thick paste I; Get safflower, be ground into meal, add 60～90 ℃ of warm lixiviates of 6～12 times of volume water and get each 0.5～2 hour 1～4 time, merge extract, filter, filtrate is crossed D-101 type macroporous absorbent resin, with 3～6 times of resinite ponding and 1～4 times of resin volume 5% alcohol flushing impurity, then use 20～40% ethanol desorbs of 2～6 times of resin volumes, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, gets thick paste II; Get the Radix Astragali, be ground into the meal of 10 mesh sieves, adding 6～12 times of volume 50～80% alcohol refluxs extracts 1～4 time, merge extract, filter, measuring relative density when filtrate is concentrated into 60 ℃ is 1.05～1.15, the water-soluble solution that adds 1～3 times of medicinal material bulking value, filter, filtrate is crossed the AB-8 large pore resin absorption column, with 3～6 times of resinite ponding and 1～4 times of resin volume 15% alcohol flushing resin post, then uses 40～70% ethanol desorbs, collect the ethanol stripping liquid, Recycled ethanol gets thick paste II I; Merge above-mentioned thick paste I, II, III, make again other preparations.
Freeze-dried powder injection in the described preparation prepares like this: get the red sage root, be ground into the meal of 10 mesh sieves, adding 8 times of volume 70% alcohol refluxs extracts 2 times, each 1 hour, merge extract, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, the water-soluble solution that adds 3 times of medicinal material volumes, filter, filtrate is crossed D-101 type macroporous absorbent resin, with 6 times of resinite ponding flushing impurity, then use 30% ethanol desorb of 4 times of resin volumes, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, gets thick paste I; Get safflower, be ground into meal, add 80 ℃ of warm lixiviates of 10 times of volume water and get each 1 hour 3 times, merge extract, filter, filtrate is crossed D-101 type macroporous absorbent resin, with 5 times of resinite ponding and 3 times of resin volume 5% alcohol flushing impurity, then use 30% ethanol desorb of 4 times of resin volumes, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, gets thick paste II; Get the Radix Astragali, be ground into the meal of 10 mesh sieves, add 8 times of volume 70% alcohol refluxs and extract 3 times, merge extract, filter, measuring relative density when filtrate is concentrated into 60 ℃ is 1.05～1.15, adds the water-soluble solution of 2 times of medicinal material bulking values, filter, filtrate is crossed the AB-8 large pore resin absorption column, with 5 times of resinite ponding and 3 times of resin volume 15% alcohol flushing resin posts, then uses 50% ethanol desorb, collect 50% ethanol stripping liquid, Recycled ethanol gets thick paste II I; Merge above-mentioned thick paste I, II, III, it is mixed, add an amount of water for injection, add again the sweet mellow wine mixing, make dissolving, add 0.3% active carbon, heating is boiled, keep little and boiled 15 minutes, cooling is filtered, filtrate adds to the full amount of water for injection, packing, pre-freeze temperature-45 ℃, pre-freeze time 10h; Begin to vacuumize, and be warming up to-40 ℃, keep 8h; Be warming up to again-35 ℃, keep 8h; Be warming up to-25 ℃, keep 8h; Be warming up to-15 ℃, keep 5h; Be warming up to 0 ℃, keep 5h; Be warming up to 10 ℃, keep 2h; Be warming up to 20 ℃, keep 2h; Be warming up to 30 ℃, keep 2h, and get final product.
Injection with small volume and concentrated solution for injection in the described preparation prepare like this: get the red sage root, be ground into the meal of 10 mesh sieves, adding 8 times of volume 70% alcohol refluxs extracts 2 times, each 1 hour, merge extract, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, the water-soluble solution that adds 3 times of medicinal material volumes, filter, filtrate is crossed D-101 type macroporous absorbent resin, with 6 times of resinite ponding flushing impurity, then use 30% ethanol desorb of 4 times of resin volumes, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, gets thick paste I; Get safflower, be ground into meal, add 80 ℃ of warm lixiviates of 10 times of volume water and get each 1 hour 3 times, merge extract, filter, filtrate is crossed D-101 type macroporous absorbent resin, with 5 times of resinite ponding and 3 times of resin volume 5% alcohol flushing impurity, then use 30% ethanol desorb of 4 times of resin volumes, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, gets thick paste II; Get the Radix Astragali, be ground into the meal of 10 mesh sieves, add 8 times of volume 70% alcohol refluxs and extract 3 times, merge extract, filter, measuring relative density when filtrate is concentrated into 60 ℃ is 1.05～1.15, adds the water-soluble solution of 2 times of medicinal material bulking values, filter, filtrate is crossed the AB-8 large pore resin absorption column, with 5 times of resinite ponding and 3 times of resin volume 15% alcohol flushing resin posts, then uses 50 % ethanol desorbs, collect 50% ethanol stripping liquid, Recycled ethanol gets thick paste II I; Above-mentioned thick paste I, II, III are mixed, add water for injection, 4 ℃ of refrigerations are spent the night, and coarse filtration, essence filter divide to install to pacify and cut open bottle, and sealing is sterilized and be get final product.
Glucose intravenous infusion in the described preparation prepares like this: get the red sage root, be ground into the meal of 10 mesh sieves, adding 8 times of volume 70% alcohol refluxs extracts 2 times, each 1 hour, merge extract, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, the water-soluble solution that adds 3 times of medicinal material volumes, filter, filtrate is crossed D-101 type macroporous absorbent resin, with 6 times of resinite ponding flushing impurity, then use 30% ethanol desorb of 4 times of resin volumes, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, gets thick paste I; Get safflower, be ground into meal, add 80 ℃ of warm lixiviates of 10 times of volume water and get each 1 hour 3 times, merge extract, filter, filtrate is crossed D-101 type macroporous absorbent resin, with 5 times of resinite ponding and 3 times of resin volume 5% alcohol flushing impurity, then use 30% ethanol desorb of 4 times of resin volumes, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, gets thick paste II; Get the Radix Astragali, be ground into the meal of 10 mesh sieves, add 8 times of volume 70% alcohol refluxs and extract 3 times, merge extract, filter, measuring relative density when filtrate is concentrated into 60 ℃ is 1.05～1.15, adds the water-soluble solution of 2 times of medicinal material bulking values, filter, filtrate is crossed the AB-8 large pore resin absorption column, with 5 times of resinite ponding and 3 times of resin volume 15% alcohol flushing resin posts, then uses 50% ethanol desorb, collect 50% ethanol stripping liquid, Recycled ethanol gets thick paste II I; Above-mentioned thick paste I, II, III are mixed, add water for injection, 4 ℃ of refrigerations are spent the night, and coarse filtration adds in an amount of glucose solution, add 0.2% active carbon and boil 15 minutes, and coarse filtration, smart filter are diluted and be get final product with water for injection.
Sodium chloride intravenous infusion in the described preparation prepares like this: get the red sage root, be ground into the meal of 10 mesh sieves, adding 8 times of volume 70% alcohol refluxs extracts 2 times, each 1 hour, merge extract, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, the water-soluble solution that adds 3 times of medicinal material volumes, filter, filtrate is crossed D-101 type macroporous absorbent resin, with 6 times of resinite ponding flushing impurity, then use 30% ethanol desorb of 4 times of resin volumes, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, gets thick paste I; Get safflower, be ground into meal, add 80 ℃ of warm lixiviates of 10 times of volume water and get each 1 hour 3 times, merge extract, filter, filtrate is crossed D-101 type macroporous absorbent resin, with 5 times of resinite ponding and 3 times of resin volume 5% alcohol flushing impurity, then use 30% ethanol desorb of 4 times of resin volumes, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, gets thick paste II; Get the Radix Astragali, be ground into the meal of 10 mesh sieves, add 8 times of volume 70% alcohol refluxs and extract 3 times, merge extract, filter, the mensuration relative density was when filtrate was concentrated into 60 ℃, 1.05～1.15, the water-soluble solution that adds 2 times of medicinal material bulking values filters, and filtrate is crossed the AB-8 large pore resin absorption column, with 5 times of resinite ponding and 3 times of resin volume 15% alcohol flushing resin posts, then use 50% ethanol desorb, collect 50% ethanol stripping liquid, Recycled ethanol gets thick paste II I; Above-mentioned thick paste I, II, III are mixed, add water for injection, 4 ℃ of refrigerations are spent the night, and coarse filtration adds in an amount of sodium chloride solution, add 0.2% active carbon and boil 15 minutes, and coarse filtration, smart filter are diluted and be get final product with water for injection.
Among the we; it is monarch drug in a prescription that Radix Astragali tonifying Qi and lifting yang, benefit are defended solid table; red sage root promoting blood circulation and cooling blood, the carbuncle that disappears are calmed the nerves; safflower activating blood to promote menstruation, stasis-dispelling and pain-killing; be ministerial drug altogether, qi and activate blood circulation has widely therapeutic action to the links of incidence of coronary heart disease and development; the circulatory system and other important functions of body are improved and regulating action; can improve heart function, coronary artery dilator improves coronary circulation; increase the Myocardial Microcirculation perfusion; reduce platelet aggregation rate, anti-bolt, thrombolysis, Ischemic myocardium; increase muscle power and reduce oxygen demand, regulate immunologic function.
Compared with prior art: the medicine of the present invention preparation is observed through experiment, has promoting blood circulation and removing blood stasisly, and promoting blood circulation, activating collaterals increases the effect of CF. Experimental result shows the obstruction of qi in the chest, the apoplexy that can be used for due to the hemostasis impatency, the treatment of the diseases such as coronary heart disease, miocardial infarction and blood stasis type pulmonary heart disease;
Among the present invention, the applicant has carried out perfect medicament composing prescription Experiment of Compatibility, not merely is simple superposition; By platelet aggregation-against test, cardiac muscle cell's culture experiment, to the red sage root: safflower: the combination prescription of the Radix Astragali minute has carried out the test of pesticide effectiveness screening of system, found that with the red sage root: safflower: the prescription pharmacological action of the Radix Astragali=1: 0.1～10: 0.1～10 combinations is strong and consumption is lower, that is to say that three's compatibility can reach the effect of synergy detoxifcation.
The side's of tearing open experimental study shows, improving myocardial ischemia, improves cardiac hemodynamic behind the Compatibility with Radix Astragali, improves microcirculation and hemorheological property, protecting liver, lowering enzymes, and it is good to strengthen the more existing preparation containing red sange root and safflower of immunization.
The medicine of producing for further proved invention has reliable curative effect, and the applicant tests:
Experimental example 1: drug regimen compatibility foundation
By platelet aggregation-against test, cardiac muscle cell's culture experiment, the result shows with part by weight and calculates: the red sage root: safflower: the Radix Astragali=1: 0.1～10: 0.1～10 combination prescription pharmacological actions are strong and consumption is lower, are the formal prescription of this project so determine this ratio prescription. Data see the following form.
The prescription screening experimental study conclusion of drug regimen compatibility
| Formula number | Prescription forms and ratio | The screening and assessment index | ||||
| The red sage root | Safflower | The Radix Astragali | Platelet aggregation rate (%) | The test of cultivation myocardial cell injury | ||
| LDH reduces % | CK reduces % | |||||
| 1 | 1 | 0.1 | 0 | 62.5％ | 30.97％ | 22.58％ |
| 1 2 3 4 5 6 7 | 1 1 1 3 3 1 1 | 0.1 1 0.5 1 1 10 10 | 0.1 1 0.8 2 0 10 0 | 51.5％ 51.4％ 50.6％ 50.9％ 59.6％ 50.4％ 59.5％ | 42.19％ 42.18％ 42.07％ 45.32％ 30.45％ 42.56％ 30.78％ | 33.51％ 34.10％ 34.26％ 35.91％ 21.30％ 34.70％ 23.06％ |
(2) side's of tearing open research conclusion
Drug efficacy study through system shows; behind the Compatibility with Radix Astragali; aspect promoting blood circulation and removing blood stasis, obvious synergistic function is arranged; aspect the treatment cerebral ischemia obvious synergistic function is being arranged also; can obviously increase simultaneously the afterload of CBF coronarius, reduction Ischemic Heart, the increase of antagonism MCO, thereby better protect ischemic myocardium. The results are shown in following table.
| Pilot project | Prescription forms and ratio | ||
| Compatibility uses | Red red group | Alone Radix Astragali group | |
| Myocardial infarction model trial drug method Myocardial Ischemia model test is improved the mouse microcirculation test and is improved the rheology test of blood stasis model rat blood due to the dog CAL method | Effect strengthens behind the compatibility effect and strengthens that effect strengthens after effect strengthens compatibility behind the compatibility behind the compatibility | Animal pattern LDH and CK and left ventricle infarction size can be significantly reduced, animal pattern HR can be significantly or apparently reduced, obvious elevation model animal MAP, obvious elevation model animal CO can obvious or remarkable elevation model animal-LV+dp/dt. LVSP obviously raises, and the MV02 of animal pattern is significantly reduced. Animal pattern LV+dp/dt and ST displacement are described the open number of remarkable increase model group Mice Auricle point of intersect of the capillary network, increase model mice auricular microcirculation velocity of blood flow, obviously improved Mice Auricle microcirculation blood fluidised form behind the injection Adr without the result without obvious effect, and promoting that Mice Auricle microcirculation third level arteries caliber obviously or significantly resists Adr behind the injection Adr shrinks the vein blood vessel effect. Can obviously, significantly or extremely significantly improve blood stasis model rat serum rheological characteristic index | Act on not obvious without the result describe open number, Mice Auricle microcirculation blood fluidised form of Mice Auricle point of intersect of the capillary network behind the injection Adr and the effect of Mice Auricle microcirculation third level arteries caliber is not obvious, can be significantly or extremely significantly increase model mice auricular microcirculation velocity of blood flow, have obviously or significantly resist Adr and shrink the vein blood vessel effect. Act on not obvious |
| Platelet aggregation-against is tested the inspection of anti-mouse tail thrombotest rabbit fibrin solubility test traditional Chinese medical science model of qi-asthenia Immune Function In Animals | Effect strengthens behind the compatibility effect and strengthens that effect strengthens after effect strengthens compatibility behind the compatibility behind the compatibility | Have and extremely significantly reduce the platelet aggregation rate effect and have and suppress extremely significantly that the thrombosis effect has fibrin in the remarkable accelerate dissolution blood plasma, extremely significantly to reduce in the blood plasma fiber protein content effect effect not obvious | Act on the not obvious obvious rising model of qi-asthenia Mouse Weight of the not obvious effect of not obvious effect, index and spleen index and thymus index |
Papillary increases without overt toxicity.
Experimental example 2: the research of preparation process
(1) red sage root process for refining
1. the selection of eluent
Because red sage root extract contains a large amount of tannins, not only can affect the stability of preparation, and can bring to the use of medicine certain trouble. The applicant adopts macroporous resin column to remove tannin. Find that in trial test the selection of eluent is the key factor that affects effective component yield. So this research has been carried out following test for the selection of eluent.
Except the investigation of tannin effect by tannin detection method under the version Chinese Pharmacopoeia appendix injection item in 2000. Get parenteral solution 1ml, add the physiological saline 5ml that contains 1% egg of new preparation, place 10min, muddiness or precipitation must not occur. Or get parenteral solution 1ml, and add 1 of spirit of vinegar, add again 4～5 of gelatin sodium chloride solutions (aqueous solution of gelatin 1%, sodium chloride 10% faces with newly joining), muddiness and precipitation must not appear.
With the ethanol wash-out of variable concentrations, investigate the loss late of root of red-rooted salvia phenolic acid in the eluent and remove the tannin effect. The result shows the ethanol elution with 60%, and the root of red-rooted salvia phenolic acid yield is high, best except the tannin effect, is eluent so select 60% ethanol.
The wash-out concentration screening
10% ethanol, 30% ethanol, 50% ethanol, 60% ethanol, 90% ethanol
Root of red-rooted salvia phenolic acid yield % 18.7 43.4 28.6 30.3 38.2
Except tannin effect bulk precipitation is clarified muddy slightly muddy slightly muddiness
2. the comparison of polyamide column and macroporous resin column elute effect
With active ingredient in polyamide column, the macroporous resin column method separation and Extraction prescription, investigate the yield of root of red-rooted salvia phenolic acid and remove the tannin effect respectively. Result: this standby good product effect of macroporous resin column legal system.
Root of red-rooted salvia phenolic acid yield (%)
1 group of 2 groups of 3 cell mean of method
Polyamide column method 64.0 62.3 64.063.2
Macroporous resin column method 75.2 71.4 73.273.1
Except the tannin effect
Method is except the tannin effect
The polyamide column method is muddy
The clarification of macroporous resin column method
(2) safflower, Radix Astragali process for refining
The wash-out concentration screening
10% ethanol, 30% ethanol, 50% ethanol, 60% ethanol, 90% ethanol
Total saponin % 18.2 33.6 48.6 34.5 38.9
Carthamin yellow yield % 25.5 43.1 40.1 35.2 18.6
(3) freeze drying conditional filtering
Experimental result shows: spray bottle, atrophy phenomenon appear in condition II gained sample, and finished product appearance character and moisture that condition I and former lyophilisation condition make all meet standard. Consider the actual conditions of production, final short condition I of selected overall time spent, i.e. freeze drying condition is: pre-freeze temperature-45 ℃, pre-freeze time 10h; Begin to vacuumize, and be warming up to-40 ℃, keep 8h; Be warming up to again-35 ℃, keep 8h; Be warming up to-25 ℃, keep 8h; Be warming up to-15 ℃, keep 5h; Be warming up to 0 ℃, keep 5h; Be warming up to 10 ℃, keep 2h; Be warming up to 20 ℃, keep 2h; Be warming up to 30 ℃, keep 2h
Concrete embodiment: (part of using among the embodiment can be gram or kilogram, ton etc.)
Embodiment 1: 30 parts of the reds sage root, 10 parts on safflower, 20 parts of the Radixs Astragali; Get the red sage root, be ground into the meal of 10 mesh sieves, adding 8 times of volume 70% alcohol refluxs extracts 2 times, each 1 hour, merge extract, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, the water-soluble solution that adds 3 times of medicinal material volumes, filter, filtrate is crossed D-101 type macroporous absorbent resin, with 6 times of resinite ponding flushing impurity, then use 30% ethanol desorb of 4 times of resin volumes, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, gets thick paste I; Get safflower, be ground into meal, add 80 ℃ of warm lixiviates of 10 times of volume water and get each 1 hour 3 times, merge extract, filter, filtrate is crossed D-101 type macroporous absorbent resin, with 5 times of resinite ponding and 3 times of resin volume 5% alcohol flushing impurity, then use 30% ethanol desorb of 4 times of resin volumes, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, gets thick paste II; Get the Radix Astragali, be ground into the meal of 10 mesh sieves, add 8 times of volume 70% alcohol refluxs and extract 3 times, merge extract, filter, measuring relative density when filtrate is concentrated into 60 ℃ is 1.05～1.15, adds the water-soluble solution of 2 times of medicinal material bulking values, filter, filtrate is crossed the AB-8 large pore resin absorption column, with 5 times of resinite ponding and 3 times of resin volume 15% alcohol flushing resin posts, then uses 50% ethanol desorb, collect 50% ethanol stripping liquid, Recycled ethanol gets thick paste II I; Above-mentioned thick paste is mixed, add an amount of water for injection, add again the sweet mellow wine mixing, make dissolving, add 0.3% active carbon, heating is boiled, and keeps little and boils 15 minutes, and cooling is filtered, filtrate adds to the full amount of water for injection, packing, pre-freeze temperature-45 ℃, pre-freeze time 10h; Begin to vacuumize, and be warming up to-40 ℃, keep 8h; Be warming up to again-35 ℃, keep 8h; Be warming up to-25 ℃, keep 8h; Be warming up to-15 ℃, keep 5h; Be warming up to 0 ℃, keep 5h; Be warming up to 10 ℃, keep 2h; Be warming up to 20 ℃, keep 2h; Be warming up to 30 ℃, keep 2h, namely get freeze-dried powder injection,, intravenous injection, three times on the one, one time one.
Embodiment 2: 30 parts of the reds sage root, 10 parts on safflower, 20 parts of the Radixs Astragali; Get the red sage root, be ground into the meal of 10 mesh sieves, adding 8 times of volume 70% alcohol refluxs extracts 2 times, each 1 hour, merge extract, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, the water-soluble solution that adds 3 times of medicinal material volumes, filter, filtrate is crossed D-101 type macroporous absorbent resin, with 6 times of resinite ponding flushing impurity, then use 30% ethanol desorb of 4 times of resin volumes, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, gets thick paste I; Get safflower, be ground into meal, add 80 ℃ of warm lixiviates of 10 times of volume water and get each 1 hour 3 times, merge extract, filter, filtrate is crossed D-101 type macroporous absorbent resin, with 5 times of resinite ponding and 3 times of resin volume 5% alcohol flushing impurity, then use 30% ethanol desorb of 4 times of resin volumes, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, gets thick paste II; Get the Radix Astragali, be ground into the meal of 10 mesh sieves, add 8 times of volume 70% alcohol refluxs and extract 3 times, merge extract, filter, measuring relative density when filtrate is concentrated into 60 ℃ is 1.05～1.15, adds the water-soluble solution of 2 times of medicinal material bulking values, filter, filtrate is crossed the AB-8 large pore resin absorption column, with 5 times of resinite ponding and 3 times of resin volume 15% alcohol flushing resin posts, then uses 50% ethanol desorb, collect 50% ethanol stripping liquid, Recycled ethanol gets thick paste II I; Above-mentioned thick paste is mixed, add water for injection, 4 ℃ of refrigerations are spent the night, and coarse filtration, smart filter divide to install to pacify and cut open bottle, and the sealing sterilization namely gets injection with small volume and concentrated solution for injection.
Embodiment 3: 30 parts of the reds sage root, 10 parts on safflower, 20 parts of the Radixs Astragali; Get the red sage root, be ground into the meal of 10 mesh sieves, adding 8 times of volume 70% alcohol refluxs extracts 2 times, each 1 hour, merge extract, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, the water-soluble solution that adds 3 times of medicinal material volumes, filter, filtrate is crossed D-101 type macroporous absorbent resin, with 6 times of resinite ponding flushing impurity, then use 30% ethanol desorb of 4 times of resin volumes, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, gets thick paste I; Get safflower, be ground into meal, add 80 ℃ of warm lixiviates of 10 times of volume water and get each 1 hour 3 times, merge extract, filter, filtrate is crossed D-101 type macroporous absorbent resin, with 5 times of resinite ponding and 3 times of resin volume 5% alcohol flushing impurity, then use 30% ethanol desorb of 4 times of resin volumes, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, gets thick paste II; Get the Radix Astragali, be ground into the meal of 10 mesh sieves, add 8 times of volume 70% alcohol refluxs and extract 3 times, merge extract, filter, measuring relative density when filtrate is concentrated into 60 ℃ is 1.05～1.15, adds the water-soluble solution of 2 times of medicinal material bulking values, filter, filtrate is crossed the AB-8 large pore resin absorption column, with 5 times of resinite ponding and 3 times of resin volume 15% alcohol flushing resin posts, then uses 50% ethanol desorb, collect 50% ethanol stripping liquid, Recycled ethanol gets thick paste II I; Above-mentioned thick paste is mixed, add water for injection, 4 ℃ of refrigerations are spent the night, and coarse filtration adds in an amount of glucose solution, add 0.2% active carbon and boil 15 minutes, and coarse filtration, smart filter namely get glucose intravenous infusion with the water for injection dilution.
Embodiment 4: 30 parts of the reds sage root, 10 parts on safflower, 20 parts of the Radixs Astragali; Get the red sage root, be ground into the meal of 10 mesh sieves, adding 8 times of volume 70% alcohol refluxs extracts 2 times, each 1 hour, merge extract, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, the water-soluble solution that adds 3 times of medicinal material volumes, filter, filtrate is crossed D-101 type macroporous absorbent resin, with 6 times of resinite ponding flushing impurity, then use 30% ethanol desorb of 4 times of resin volumes, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, gets thick paste I; Get safflower, be ground into meal, add 80 ℃ of warm lixiviates of 10 times of volume water and get each 1 hour 3 times, merge extract, filter, filtrate is crossed D-101 type macroporous absorbent resin, with 5 times of resinite ponding and 3 times of resin volume 5% alcohol flushing impurity, then use 30% ethanol desorb of 4 times of resin volumes, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, gets thick paste II; Get the Radix Astragali, be ground into the meal of 10 mesh sieves, add 8 times of volume 70% alcohol refluxs and extract 3 times, merge extract, filter, measuring relative density when filtrate is concentrated into 60 ℃ is 1.05～1.15, adds the water-soluble solution of 2 times of medicinal material bulking values, filter, filtrate is crossed the AB-8 large pore resin absorption column, with 5 times of resinite ponding and 3 times of resin volume 15% alcohol flushing resin posts, then uses 50% ethanol desorb, collect 50% ethanol stripping liquid, Recycled ethanol gets thick paste II I; Above-mentioned thick paste is mixed, add water for injection, 4 ℃ of refrigerations are spent the night, and coarse filtration adds in an amount of sodium chloride solution, add 0.2% active carbon and boil 15 minutes, and coarse filtration, smart filter namely get sodium chloride intravenous infusion with the water for injection dilution.
Embodiment 5: 10 parts of the reds sage root, 1 part on safflower, 1 part of the Radix Astragali; Get the red sage root, be ground into the meal of 10 mesh sieves, adding 6 times of volume 50% alcohol refluxs extracted 0.5 hour, merge extract, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, the water-soluble solution that adds 2 times of medicinal material volumes, filter, filtrate is crossed D-101 type macroporous absorbent resin, with 3～10 times of resinite ponding flushing impurity, then use 20% ethanol desorb of 2 times of resin volumes, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, gets thick paste I; Get safflower, be ground into meal, add 60 ℃ of warm lixiviates of 6 times of volume water and got 0.5 hour, merge extract, filter, filtrate is crossed D-101 type macroporous absorbent resin, with 3 times of resinite ponding and 1 times of resin volume 5% alcohol flushing impurity, then use 20% ethanol desorb of 2 times of resin volumes, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, gets thick paste II; Get the Radix Astragali, be ground into the meal of 10 mesh sieves, add 6 times of volume 50% alcohol refluxs and extract 1 time, merge extract, filter, measuring relative density when filtrate is concentrated into 60 ℃ is 1.05～1.15, adds the water-soluble solution of 1 times of medicinal material bulking value, filter, filtrate is crossed the AB-8 large pore resin absorption column, with 3 times of resinite ponding and 1 times of resin volume 15% alcohol flushing resin post, then uses 40% ethanol desorb, collect the ethanol stripping liquid, Recycled ethanol gets thick paste II I; Drying is pulverized, and compressing tablet namely gets tablet.
Embodiment 6: 10 parts of the reds sage root, 5 parts on safflower, 8 parts of the Radixs Astragali; Get the red sage root, be ground into the meal of 10 mesh sieves, adding 10 times of volume 90% alcohol refluxs extracts 4 times, each 4 hours, merge extract, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, the water-soluble solution that adds 4 times of medicinal material volumes, filter, filtrate is crossed D-101 type macroporous absorbent resin, with 10 times of resinite ponding flushing impurity, then use 50% ethanol desorb of 8 times of resin volumes, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, gets thick paste I; Get safflower, be ground into meal, add 60～90 ℃ of warm lixiviates of 12 times of volume water and get each 2 hours 4 times, merge extract, filter, filtrate is crossed D-101 type macroporous absorbent resin, with 6 times of resinite ponding and 4 times of resin volume 5% alcohol flushing impurity, then use 40% ethanol desorb of 6 times of resin volumes, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, gets thick paste II; Get the Radix Astragali, be ground into the meal of 10 mesh sieves, add 12 times of volume 80% alcohol refluxs and extract 4 times, merge extract, filter, measuring relative density when filtrate is concentrated into 60 ℃ is 1.05～1.15, adds the water-soluble solution of 3 times of medicinal material bulking values, filter, filtrate is crossed the AB-8 large pore resin absorption column, with 6 times of resinite ponding and 4 times of resin volume 15% alcohol flushing resin posts, then uses 70% ethanol desorb, collect the ethanol stripping liquid, Recycled ethanol gets thick paste II I; Drying is pulverized, and granulates, and namely gets granule.
Embodiment 7: 10 parts of the reds sage root, 10 parts on safflower, 10 parts of the Radixs Astragali; Get the red sage root, be ground into meal, add alcohol extract, measuring relative density during extract decompression recycling ethanol to 60 ℃ is 1.05～1.15, adopt again one or more methods in flocculent precipitation, extraction, depositing in water method and the column chromatography to unite to use and make with extra care, get thick paste I; Get safflower, be ground into meal, measuring relative density when extracting in water, extract are evaporated to 60 ℃ is 1.05～1.15, adopt again one or more methods in alcohol deposition method, extraction, flocculent precipitation and the column chromatography to unite to use and make with extra care, get thick paste II; Get the Radix Astragali, be ground into the meal of 10 mesh sieves, add alcohol extract, extract filters, measuring relative density when filtrate is concentrated into 60 ℃ is 1.05～1.15, adopt again one or more methods in depositing in water method, flocculent precipitation, extraction and the column chromatography to unite to use and make with extra care, get thick paste II I; Merge above-mentioned thick paste I, II, III, add distilled water, namely get oral liquid.
Embodiment 8: 10 parts of the reds sage root, 100 parts on safflower, 100 parts of the Radixs Astragali; Get the red sage root, safflower, the Radix Astragali, pulverize, water or ethanolic solution extract separately, make with extra care respectively, merge extract, and drying is pulverized, and granulates, and is encapsulated, namely gets capsule.
Embodiment 9: 10 parts of Salvia root P.Es, 100 parts of safflower extracts, 100 parts of Astragalus Root P.Es; Get commercially available Salvia root P.E, commercially available safflower extract, commercially available Astragalus Root P.E, drying is pulverized, and granulates, and is encapsulated, namely gets capsule.
Embodiment 10: 10 parts of Salvia root P.Es, 1 part of safflower extract, 1 part of Astragalus Root P.E; Get commercially available Salvia root P.E, commercially available safflower extract, commercially available Astragalus Root P.E, drying is pulverized, and granulates, and namely gets granule.
Claims (13)
1. Chinese medicine preparation for the treatment of cardiovascular and cerebrovascular disease, it is characterized in that: calculate with part by weight: it is by the red sage root: safflower: the Radix Astragali=1: 0.1～10: 0.1～10 or the preparation made from the extract of the medicinal material of corresponding weight ratio.
2, according to the Chinese medicine preparation for the treatment of cardiovascular and cerebrovascular disease claimed in claim 1, it is characterized in that: calculate with part by weight: it is by the red sage root: safflower: the Radix Astragali=1: 0.1～1: 0.1～1 or the preparation made from the extract of the medicinal material of corresponding weight ratio.
3, according to the Chinese medicine preparation for the treatment of cardiovascular and cerebrovascular disease claimed in claim 2, it is characterized in that: calculate with part by weight: it is by the red sage root: safflower: the Radix Astragali=1: 0.1～0.5: 0.1～0.8 or the preparation made from the extract of the medicinal material of corresponding weight ratio.
4, according to the Chinese medicine preparation for the treatment of cardiovascular and cerebrovascular disease claimed in claim 3, it is characterized in that: calculate with part by weight: it is by the red sage root: safflower: the preparation that the extract of the medicinal material of the Radix Astragali=3: 1: 2 or corresponding weight ratio is made.
5, according to the Chinese medicine preparation of the described treatment cardiovascular and cerebrovascular disease of any one in the claim 1～4, it is characterized in that: described preparation is injection, capsule, soft capsule, micro-capsule, granule, pill, dripping pill, sustained release preparation, controlled release preparation, quick releasing formulation, targeting preparation, oral liquid, syrup, powder or mixture.
6, according to the Chinese medicine preparation for the treatment of cardiovascular and cerebrovascular disease claimed in claim 5, it is characterized in that: described injection comprise the injection with small volume that is directly used in drug administration by injection, directly for the venous transfusion of drip-feed, need to be used for after the dilution concentrated solution for injection and the injection sterile powder of drip-feed.
7, such as the preparation method of the Chinese medicine preparation of the described treatment cardiovascular and cerebrovascular disease of any one in the claim 1～6, it is characterized in that: get the red sage root, safflower, the Radix Astragali, pulverize, water or ethanolic solution extract separately, make with extra care respectively, merge extract, be prepared into different preparations.
8, according to the preparation method of the Chinese medicine preparation for the treatment of cardiovascular and cerebrovascular disease claimed in claim 7, it is characterized in that: get the red sage root, be ground into meal, add alcohol extract, measuring relative density during extract decompression recycling ethanol to 60 ℃ is 1.05～1.15, adopt again one or more methods in flocculent precipitation, extraction, depositing in water method or the column chromatography to unite to use and make with extra care, get thick paste I; Get safflower, be ground into meal, measuring relative density when extracting in water, extract are evaporated to 60 ℃ is 1.05～1.15, adopt again one or more methods in alcohol deposition method, extraction, flocculent precipitation or the column chromatography to unite to use and make with extra care, get thick paste II; Get the Radix Astragali, be ground into the meal of 10 mesh sieves, add alcohol extract, extract filters, measuring relative density when filtrate is concentrated into 60 ℃ is 1.05～1.15, adopt again one or more methods in depositing in water method, flocculent precipitation, extraction or the column chromatography to unite to use and make with extra care, get thick paste II I; Merge above-mentioned thick paste I, II, III, make different preparations.
9, according to the preparation method of the Chinese medicine preparation for the treatment of cardiovascular and cerebrovascular disease claimed in claim 8, it is characterized in that: get the red sage root, be ground into the meal of 10 mesh sieves, adding 6～10 times of volume 50%～90% alcohol refluxs extracts 1～4 time, each 0.5～4 hour, merge extract, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, the water-soluble solution that adds 2～4 times of medicinal material volumes, filter, filtrate is crossed D-101 type macroporous absorbent resin, with 3～10 times of resinite ponding flushing impurity, then use 20%～50% ethanol desorb of 2～8 times of resin volumes, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, gets thick paste I; Get safflower, be ground into meal, add 60～90 ℃ of warm lixiviates of 6～12 times of volume water and get each 0.5～2 hour 1～4 time, merge extract, filter, filtrate is crossed D-101 type macroporous absorbent resin, with 3～6 times of resinite ponding and 1～4 times of resin volume 5 % alcohol flushing impurity, then use 20～40% ethanol desorbs of 2～6 times of resin volumes, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, gets thick paste II; Get the Radix Astragali, be ground into the meal of 10 mesh sieves, adding 6～12 times of volume 50～80 % alcohol refluxs extracts 1～4 time, merge extract, filter, measuring relative density when filtrate is concentrated into 60 ℃ is 1.05～1.15, the water-soluble solution that adds 1～3 times of medicinal material bulking value, filter, filtrate is crossed the AB-8 large pore resin absorption column, with 3～6 times of resinite ponding and 1～4 times of resin volume 15% alcohol flushing resin post, then uses 40～70% ethanol desorbs, collect the ethanol stripping liquid, Recycled ethanol gets thick paste II I; Merge above-mentioned thick paste I, II, III, make again other preparations.
10 preparation methods according to the Chinese medicine preparation for the treatment of cardiovascular and cerebrovascular disease claimed in claim 9, it is characterized in that: the freeze-dried powder injection in the described preparation prepares like this: get the red sage root, be ground into the meal of 10 mesh sieves, adding 8 times of volume 70% alcohol refluxs extracts 2 times, each 1 hour, merge extract, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, the water-soluble solution that adds 3 times of medicinal material volumes, filter, filtrate is crossed D-101 type macroporous absorbent resin, with 6 times of resinite ponding flushing impurity, then use 30% ethanol desorb of 4 times of resin volumes, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, gets thick paste I; Get safflower, be ground into meal, add 80 ℃ of warm lixiviates of 10 times of volume water and get each 1 hour 3 times, merge extract, filter, filtrate is crossed D-101 type macroporous absorbent resin, with 5 times of resinite ponding and 3 times of resin volume 5% alcohol flushing impurity, then use 30% ethanol desorb of 4 times of resin volumes, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, gets thick paste II; Get the Radix Astragali, be ground into the meal of 10 mesh sieves, add 8 times of volume 70% alcohol refluxs and extract 3 times, merge extract, filter, measuring relative density when filtrate is concentrated into 60 ℃ is 1.05～1.15, adds the water-soluble solution of 2 times of medicinal material bulking values, filter, filtrate is crossed the AB-8 large pore resin absorption column, with 5 times of resinite ponding and 3 times of resin volume 15% alcohol flushing resin posts, then uses 50% ethanol desorb, collect 50% ethanol stripping liquid, Recycled ethanol gets thick paste II I; Merge above-mentioned thick paste I, II, III, it is mixed, add an amount of water for injection, add again the sweet mellow wine mixing, make dissolving, add 0.3% active carbon, heating is boiled, keep little and boiled 15 minutes, cooling is filtered, filtrate adds to the full amount of water for injection, packing, pre-freeze temperature-45 ℃, pre-freeze time 10h; Begin to vacuumize, and be warming up to-40 ℃, keep 8h; Be warming up to again-35 ℃, keep 8h; Be warming up to-25 ℃, keep 8h; Be warming up to-15 ℃, keep 5h; Be warming up to 0 ℃, keep 5h; Be warming up to 10 ℃, keep 2h; Be warming up to 20 ℃, keep 2h; Be warming up to 30 ℃, keep 2h, and get final product.
11, according to the preparation method of the Chinese medicine preparation for the treatment of cardiovascular and cerebrovascular disease claimed in claim 9, it is characterized in that: injection with small volume and concentrated solution for injection in the described preparation prepare like this: get the red sage root, be ground into the meal of 10 mesh sieves, adding 8 times of volume 70% alcohol refluxs extracts 2 times, each 1 hour, merge extract, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, the water-soluble solution that adds 3 times of medicinal material volumes, filter, filtrate is crossed D-101 type macroporous absorbent resin, with 6 times of resinite ponding flushing impurity, then use 30% ethanol desorb of 4 times of resin volumes, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, gets thick paste I; Get safflower, be ground into meal, add 80 ℃ of warm lixiviates of 10 times of volume water and get each 1 hour 3 times, merge extract, filter, filtrate is crossed D-101 type macroporous absorbent resin, with 5 times of resinite ponding and 3 times of resin volume 5 % alcohol flushing impurity, then use 30% ethanol desorb of 4 times of resin volumes, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, gets thick paste II; Get the Radix Astragali, be ground into the meal of 10 mesh sieves, add 8 times of volume 70% alcohol refluxs and extract 3 times, merge extract, filter, measuring relative density when filtrate is concentrated into 60 ℃ is 1.05～1.15, adds the water-soluble solution of 2 times of medicinal material bulking values, filter, filtrate is crossed the AB-8 large pore resin absorption column, with 5 times of resinite ponding and 3 times of resin volume 15% alcohol flushing resin posts, then uses 50% ethanol desorb, collect 50% ethanol stripping liquid, Recycled ethanol gets thick paste II I; Above-mentioned thick paste I, II, III are mixed, add water for injection, 4 ℃ of refrigerations are spent the night, and coarse filtration, essence filter divide to install to pacify and cut open bottle, and sealing is sterilized and be get final product.
12, according to the preparation method of the Chinese medicine preparation for the treatment of cardiovascular and cerebrovascular disease claimed in claim 9, it is characterized in that: the glucose intravenous infusion in the described preparation prepares like this: get the red sage root, be ground into the meal of 10 mesh sieves, adding 8 times of volume 70% alcohol refluxs extracts 2 times, each 1 hour, merge extract, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, the water-soluble solution that adds 3 times of medicinal material volumes, filter, filtrate is crossed D-101 type macroporous absorbent resin, with 6 times of resinite ponding flushing impurity, then use 30% ethanol desorb of 4 times of resin volumes, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, gets thick paste I; Get safflower, be ground into meal, add 80 ℃ of warm lixiviates of 10 times of volume water and get each 1 hour 3 times, merge extract, filter, filtrate is crossed D-101 type macroporous absorbent resin, with 5 times of resinite ponding and 3 times of resin volume 5% alcohol flushing impurity, then use 30% ethanol desorb of 4 times of resin volumes, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, gets thick paste II; Get the Radix Astragali, be ground into the meal of 10 mesh sieves, add 8 times of volume 70% alcohol refluxs and extract 3 times, merge extract, filter, measuring relative density when filtrate is concentrated into 60 ℃ is 1.05～1.15, adds the water-soluble solution of 2 times of medicinal material bulking values, filter, filtrate is crossed the AB-8 large pore resin absorption column, with 5 times of resinite ponding and 3 times of resin volume 15% alcohol flushing resin posts, then uses 50% ethanol desorb, collect 50% ethanol stripping liquid, Recycled ethanol gets thick paste II I; Above-mentioned thick paste I, II, III are mixed, add water for injection, 4 ℃ of refrigerations are spent the night, and coarse filtration adds in an amount of glucose solution, add 0.2% active carbon and boil 15 minutes, and coarse filtration, smart filter are diluted and be get final product with water for injection.
13, according to the preparation method of the Chinese medicine preparation for the treatment of cardiovascular and cerebrovascular disease claimed in claim 9, it is characterized in that: the sodium chloride intravenous infusion in the described preparation prepares like this: get the red sage root, be ground into the meal of 10 mesh sieves, adding 8 times of volume 70% alcohol refluxs extracts 2 times, each 1 hour, merge extract, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, the water-soluble solution that adds 3 times of medicinal material volumes, filter, filtrate is crossed D-101 type macroporous absorbent resin, with 6 times of resinite ponding flushing impurity, then use 30% ethanol desorb of 4 times of resin volumes, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, gets thick paste I; Get safflower, be ground into meal, add 80 ℃ of warm lixiviates of 10 times of volume water and get each 1 hour 3 times, merge extract, filter, filtrate is crossed D-101 type macroporous absorbent resin, with 5 times of resinite ponding and 3 times of resin volume 5% alcohol flushing impurity, then use 30% ethanol desorb of 4 times of resin volumes, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, gets thick paste II; Get the Radix Astragali, be ground into the meal of 10 mesh sieves, add 8 times of volume 70% alcohol refluxs and extract 3 times, merge extract, filter, measuring relative density when filtrate is concentrated into 60 ℃ is 1.05～1.15, adds the water-soluble solution of 2 times of medicinal material bulking values, filter, filtrate is crossed the AB-8 large pore resin absorption column, with 5 times of resinite ponding and 3 times of resin volume 15% alcohol flushing resin posts, then uses 50% ethanol desorb, collect 50% ethanol stripping liquid, Recycled ethanol gets thick paste II I; Above-mentioned thick paste I, II, III are mixed, add water for injection, 4 ℃ of refrigerations are spent the night, and coarse filtration adds in an amount of sodium chloride solution, add 0.2% active carbon and boil 15 minutes, and coarse filtration, smart filter are diluted and be get final product with water for injection.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101049338B (en) * | 2006-04-05 | 2011-05-04 | 山西亚宝药业集团股份有限公司 | Composition of Chinese traditional medicine for treating cardiovascular disease and cerebrovascular disease |
| CN105497790A (en) * | 2015-12-28 | 2016-04-20 | 高春喜 | Medicine composition for preventing and treating cardiovascular and cerebrovascular infarction |
| CN109224059A (en) * | 2018-10-26 | 2019-01-18 | 湛江市通灵医学生物工程有限公司 | compound preparation and preparation method thereof |
| CN110075222A (en) * | 2019-05-17 | 2019-08-02 | 王兴东 | A kind of logical Chinese materia medica preparation of heart and brain |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101049338B (en) * | 2006-04-05 | 2011-05-04 | 山西亚宝药业集团股份有限公司 | Composition of Chinese traditional medicine for treating cardiovascular disease and cerebrovascular disease |
| CN105497790A (en) * | 2015-12-28 | 2016-04-20 | 高春喜 | Medicine composition for preventing and treating cardiovascular and cerebrovascular infarction |
| CN109224059A (en) * | 2018-10-26 | 2019-01-18 | 湛江市通灵医学生物工程有限公司 | compound preparation and preparation method thereof |
| CN110075222A (en) * | 2019-05-17 | 2019-08-02 | 王兴东 | A kind of logical Chinese materia medica preparation of heart and brain |
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