CN1891249A - Chinese medicine preparation for treating cardio-cerebrovascular disease and its preparing method - Google Patents
Chinese medicine preparation for treating cardio-cerebrovascular disease and its preparing method Download PDFInfo
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- CN1891249A CN1891249A CN 200610200651 CN200610200651A CN1891249A CN 1891249 A CN1891249 A CN 1891249A CN 200610200651 CN200610200651 CN 200610200651 CN 200610200651 A CN200610200651 A CN 200610200651A CN 1891249 A CN1891249 A CN 1891249A
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Abstract
The present invention relates to a Chinese medicine preparation for curing angiocardiopathy and cerebrovascular disease. Said Chinese preparation is made up by using Chinese medicinal materials of erigeron breviscapus, Chinese angelica root and notoginseng root through a certain preparation process.
Description
Technical field: the present invention is a kind of Chinese medicine preparation for the treatment of cardiovascular and cerebrovascular disease and preparation method thereof, belongs to technical field of Chinese medicine.
Technical background: we suit the medicine to the illness--and-cardiovascular and cerebrovascular disease is a big persistent ailment that threatens world's healthy population as coronary heart disease, myocardial infarction, rhomboembolia type cerebrovascular, and the trend that progressively rises is arranged in recent years.The height of cardiovascular and cerebrovascular disease mortality rate is to weigh a country, resident's quality of life, the important indicator of sanitary health career, along with the arriving of fairly comfortable life, and the change of meals spectrum, resident's longevity, healthy existence desire improve constantly.In order to prevent, control the generation of cardiovascular and cerebrovascular disease, exploitation cardiovascular and cerebrovascular disease class medicine has become a main trend.So far existing a large amount of Chinese medicine and western medicine emerge.But the side effect of Western medicine is big, and Chinese medicine is long the course of treatment.Therefore, it is low to seek a kind of cost, determined curative effect, and the natural Chinese medicines preparation that has no side effect is very urgent, contrast after deliberation, we develop a kind of Chinese medicine preparation for the treatment of cardiovascular and cerebrovascular disease, by Herba Erigerontis, when being grouped into.Up to now, less than report with both compatibility agents.And through affirmation and the side's of tearing open the experimental study to this combination prescription effectiveness, the result shows, adds Herba Erigerontis, Radix Angelicae Sinensis, the Radix Notoginseng prescription formed behind the Radix Notoginseng and improves myocardial ischemia, improves the better effects if of cardiac hemodynamic.
Summary of the invention: the objective of the invention is to: utilize Herba Erigerontis, Radix Angelicae Sinensis blood circulation promoting and blood stasis dispelling, both are combined into novel formulation develop research, bring into play both in the cooperative compensating effect aspect the angina pectoris by compatibility, select for this type of treatment of diseases increases a new medication.Simultaneously, more excellent through system further investigation Herba Erigerontis, Radix Angelicae Sinensis, Radix Notoginseng three compatibility curative effect.And its effective site or extract formulation system employing advanced technologies are purified to medicine and are made with extra care, and adopt effectively to become content that its quality is controlled, and make active ingredient carry out enrichment, and curative effect is more outstanding.
The present invention constitutes like this: the Chinese medicine preparation of treatment cardiovascular and cerebrovascular disease: calculate according to components by weight percent, it by 99～1 parts of 1～99 part of Herba Erigerontis and Radix Angelicae Sinensis through extracting refining forming; Or be made with Radix Angelicae Sinensis extract or the effective site that corresponding weight portion medical material obtains after extraction by Herba Erigerontis extract or the effective site that corresponding weight portion medical material obtains after extracting.
Specifically: calculate according to components by weight percent, it by 99～1 parts of 1～99 part of Herba Erigerontis, Radix Angelicae Sinensis and Radix Notoginseng through extracting refining forming; Or the extract or the effective site that are obtained after extracting by corresponding weight portion medical material are made.
More specific: calculate according to parts by weight, it is made for 0～60 part by 20～80 parts of Herba Erigerontiss, 10～70 parts of Radix Angelicae Sinensis, Radix Notoginseng, or the extract or the effective site that are obtained after extracting by corresponding weight portion medical material are made.
Accurately: calculate according to parts by weight, it is made for 20 parts by 50 parts of Herba Erigerontiss, 30 parts of Radix Angelicae Sinensis, Radix Notoginseng, or the extract or the effective site that are obtained after extracting by corresponding weight portion medical material are made.
Among the present invention, contain scutellarin in the Herba Erigerontis extract and be not less than 3%, contain ferulic acid in the Radix Angelicae Sinensis extract and be not less than 0.5%, contain arasaponin R1, ginsenoside Rb in the Radix Notoginseng extract
1And ginsenoside Rg
1Total amount be not less than 3%.
Contain flavones ingredient in the effective site Herba Erigerontis total flavones of Herba Erigerontis and be not less than 50%, contain saponin component in the effective site Radix Notoginseng total arasaponins of Radix Notoginseng and be not less than 50%, contain organic acids composition in the effective site Radix Angelicae Sinensis total acid of Radix Angelicae Sinensis and be not less than 50%.
Described preparation is injection and oral formulations; Injection comprises: be directly used in the injection of drug administration by injection, directly supply the venous transfusion of intravenous drip, need be used for the concentrated solution for injection of intravenous drip and injectable sterile powder and the aseptic block that makes with freeze-drying or spray drying method after diluting; Oral formulations comprises: all acceptable dosage forms on the pharmaceuticss such as tablet, dispersible tablet, effervescent tablet, oral cavity disintegration tablet, capsule, soft capsule, microcapsule, granule, pill, powder, drop pill, slow releasing preparation, controlled release preparation, oral liquid, soft extract, extractum, gel, membrane.
The preparation method of the Chinese medicine preparation of treatment cardiovascular and cerebrovascular disease: get Herba Erigerontis, ethanol extraction, the extracting solution decompression recycling ethanol adopts in column chromatography, extraction, the sedimentation method one or more to make with extra care on this basis, Herba Erigerontis extract or effective site; Get Radix Notoginseng, ethanol extraction, the extracting solution decompression recycling ethanol adopts in column chromatography, extraction, the sedimentation method one or more to make with extra care on this basis, Radix Notoginseng extract or effective site; Get Radix Angelicae Sinensis, decocting boils, collecting decoction, filter, filtrate concentrates, and adopts in column chromatography, extraction, the sedimentation method one or more to make with extra care on this basis, get Radix Angelicae Sinensis extract or effective site, merge above-mentioned each extract or effective site, make different preparations.
Concrete: get Herba Erigerontis, be ground into the coarse powder of 10 mesh sieves, add 6～12 times of volume 50～80% alcohol reflux 1～4 time, each 0.5～3 hour, merge extractive liquid,, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, the water heating for dissolving that adds 1～4 times of medical material volume, filter, filtrate is crossed resin column, with 2～7 times of resinite hydrops and 1～4 times of resin volume 5% alcohol flushing impurity, use 30% alcohol desorption of 2～8 times of resin volumes then, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, Herba Erigerontis extract; Get Radix Notoginseng, be ground into the coarse powder of 10 mesh sieves, add 6～12 times of volume 50～80% alcohol reflux 1～4 time, each 0.5～3 hour, merge extractive liquid,, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, the water dissolution that adds 1～4 times of medical material volume, filter, filtrate is crossed resin column, with 2～8 times of resinite hydrops and 1～4 times of resin volume 15% alcohol flushing impurity, use 60% alcohol desorption of 2～8 times of resin volumes then, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, Radix Notoginseng extract; Get Radix Angelicae Sinensis, be ground into the coarse powder of 10 mesh sieves, and added 6～12 times of decoctings and boil collecting decoction 1～4 time, filter, measuring relative density when filtrate is concentrated into 60 ℃ is 1.05～1.15, adds ethanol and makes and contain the alcohol amount and reach 50～80%, and cold preservation is spent the night, filter, measuring relative density during filtrate recycling ethanol to 60 ℃ is 1.05～1.15, adds the water dissolution of 1～3 times of medical material bulking value, filters, filtrate is crossed resin column, with 3～8 times of resinite hydrops flushing resin columns, use 2 ~ 6 times of resin volume 25% alcohol desorptions then, collect 25% ethanolysis imbibition, reclaim ethanol and get Radix Angelicae Sinensis extract 1, with the precipitation behind the precipitate with ethanol, add 1～4 times of amount water for injection again, stir evenly, filter, filtrate concentrate Radix Angelicae Sinensis extract 2, merge above-mentioned Herba Erigerontis extract, Radix Notoginseng extract, Radix Angelicae Sinensis extract 1 and 2 is made different preparations.
Freeze-dried powder injection in the described preparation prepares like this: get Herba Erigerontis, be ground into the coarse powder of 10 mesh sieves, add 8 times of volume 70% alcohol reflux 3 times, each 1 hour, merge extractive liquid,, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, the water heating for dissolving that adds 3 times of medical material volumes, filter, filtrate is crossed ZTC-1 type macroporous adsorbent resin, with 5 times of resinite hydrops and 3 times of resin volume 5% alcohol flushing impurity, use 30% alcohol desorption of 4 times of resin volumes then, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, Herba Erigerontis extract; Get Radix Notoginseng, be ground into the coarse powder of 10 mesh sieves, add 8 times of volume 70% alcohol reflux 3 times, each 1 hour, merge extractive liquid,, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, the water dissolution that adds 3 times of medical material volumes, filter, filtrate is crossed ZTC-1 type macroporous adsorbent resin, with 4 times of resinite hydrops and 3 times of resin volume 15% alcohol flushing impurity, use 60% alcohol desorption of 4 times of resin volumes then, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, Radix Notoginseng extract; Get Radix Angelicae Sinensis, be ground into the coarse powder of 10 mesh sieves, adding 8 times of decoctings boils 3 times, collecting decoction, filter, measuring relative density when filtrate is concentrated into 60 ℃ is 1.05～1.15, adds ethanol and makes and contain the alcohol amount and reach 70%, cold preservation is spent the night, filter, measuring relative density during filtrate recycling ethanol to 60 ℃ is 1.05～1.15, adds the water dissolution of 2 times of medical material bulking values, filter, filtrate is crossed the ZTC-1 macroporous adsorptive resins, with 5 times of resinite hydrops flushing resin columns, uses 4 times of resin volume 25% alcohol desorptions then, collect 25% ethanolysis imbibition, reclaim ethanol and get Radix Angelicae Sinensis extract 1; With the precipitation behind the precipitate with ethanol, add 3 times of amount waters for injection again, stir evenly, filter, filtrate concentrate Radix Angelicae Sinensis extract 2, above-mentioned Herba Erigerontis extract is added an amount of water for injection, heating, sodium hydroxide solution with 40%, adjusting pH value is 6.5～7.0, makes dissolving, adds above-mentioned Radix Notoginseng extract, Radix Angelicae Sinensis extract 1 and 2 and the glucose mixing, add water for injection, add 0.3% active carbon again, heated and boiled keeps little and boiled 15 minutes, cooling, filter, filtrate adds to the full amount of water for injection, packing, lyophilization, promptly.
Among the we, Herba Erigerontis, Radix Angelicae Sinensis blood circulation promoting and blood stasis dispelling can make the cardiac function of core function badness improve, and antithrombotic forms and improves hemorheology, and microcirculation improvement is usually used in the treatment of coronary heart disease.Both share disperse blood stasis and dredge collateral, reach the therapeutical effect to coronary heart disease.
Compared with prior art, the applicant has carried out perfect medicament composing prescription compatibility experiment, not merely is simple superposition; By antiplatelet aggregation test, myocardial cell culture experiment, to Herba Erigerontis: Radix Angelicae Sinensis divides the test of pesticide effectiveness of having carried out system screening by 5: 1,5: 2,5: 3,5: 4 and 1: 5 five combination prescription, found that with Herba Erigerontis: the prescription pharmacological action of Radix Angelicae Sinensis combination in 5: 3 is strong and consumption is lower, that is to say that both compatibilities can reach potentiation antidotal effect.Also to the carrying out of this combination prescription effectiveness confirm and the side's of tearing open experimental study that the result shows, improving myocardial ischemia after adding Radix Notoginseng, improve the better effects if of cardiac hemodynamic.
Find that in the process of preparation injection we find that Herba Erigerontis extract is a flavones ingredient in the process of preparation injection, can not dissolve fully in water.So need in this product, add the pH regulator agent, be controlled at a suitable PH scope, can improve the stability of injection formulation effectively, make quality controllable, stable.
The applicant has carried out a series of experiments, can prove that medicine provided by the invention has effective effect;
Experimental example 1: drug regimen compatibility foundation
By antiplatelet aggregation test, myocardial cell culture experiment, to Herba Erigerontis: Radix Angelicae Sinensis: Radix Notoginseng is divided by 5: 1: 0,5: 2: 1,5: 3: 2,5: 4: 3 and 1: 5: 0 five combination prescription and has carried out the test of pesticide effectiveness screening study of system, the result shows Herba Erigerontis: Radix Angelicae Sinensis: Radix Notoginseng=5: 3: 2 combination prescriptions pharmacological action is stronger, is the formal prescription of this project so determine this ratio prescription.Data see the following form.
The prescription screening experimental study conclusion of drug regimen compatibility
| Formula number | The screening and assessment index | |||||
| Herba Erigerontis | Radix Angelicae Sinensis | Radix Notoginseng | Platelet aggregation rate (%) | The test of cultivation myocardial cell injury | ||
| LDH reduces % | CK reduces % | |||||
| 1 | 5 | 1 | 0 | 68.5％ | 36.19％ | 24.51％ |
| 2 | 5 | 2 | 1 | 57.6％ | 32.11％ | 34.10％ |
| 3 | 5 | 3 | 2 | 50.6％ | 46.02％ | 51.25％ |
| 4 | 5 | 4 | 3 | 59.7％ | 43.22％ | 35.38％ |
| 5 | 1 | 5 | 0 | 53.2％ | 27.40％ | 21.30％ |
(2) side's of tearing open research conclusion
Drug efficacy study through system shows; after two medication combined application; aspect blood circulation promoting and blood stasis dispelling, obvious role in synergism is arranged; aspect the treatment cerebral ischemia obvious role in synergism is being arranged also; adding Radix Notoginseng can also obviously increase the afterload of blood flow coronarius, reduction Ischemic Heart, the increase of antagonism myocardial oxygen consumption, thus the better protection ischemic myocardium.The results are shown in following table.
| Pilot project | Prescription is formed and ratio | ||||
| Herba Erigerontis and Radix Angelicae Sinensis compatibility use | Single Herba Erigerontis group of using | Single with working as grouping | |||
| Myocardial infarction model test due to the dog coronary artery ligation method | Effect strengthens behind the two compatibility, can significantly reduce animal pattern LDH and CK and left ventricle infarction size | Can reduce animal pattern LDH and CK and left ventricle infarction size | The trend that reduces animal pattern LDH and CK and left ventricle infarction size is arranged | ||
| Improve the rheology test of blood stasis model rat blood | Effect strengthens behind the two compatibility, can significantly improve blood stasis model rat serum rheological characteristic index | Can obviously improve blood stasis model rat serum rheological characteristic index | Not obvious to the effect of blood stasis model rat serum rheological characteristic index | ||
| Pilot project | Prescription is formed and ratio | ||||
| Three's compatibility uses | Oil lamp, work as grouping | Single with working as grouping | |||
| The antiplatelet aggregation test | Effect strengthens behind three's compatibility, can significantly reduce platelet aggregation rate | The effect that reduces platelet aggregation rate is arranged | Act on not obvious | ||
| Anti-mouse tail thrombotest | Effect strengthens behind three's compatibility, can significantly suppress thrombosis | Can obviously suppress thrombosis | The thrombotic effect of certain inhibition is arranged |
| The rabbit fibrin solubility test | Effect strengthens behind three's compatibility, fibrin in the remarkable accelerate dissolution blood plasma is arranged, reduce fiber protein content effect in the blood plasma | Can reduce fiber protein content effect in the blood plasma | Act on not obvious |
The selection of experimental example 2 dosing pH value
Dissolve best pH: the pH value of our regulator solution, the preferable dissolving situation of research Herba Erigerontis extract.
Herba Erigerontis extract dissolves best pH
| Herba Erigerontis extract consumption (mg) | Liquor capacity (ml) | PH value | Clarity |
| 50 | 100 | 5.5 | Muddy |
| 50 | 100 | 6.0 | Muddy |
| 50 | 100 | 6.5 | Clarification |
| 50 | 100 | 7.0 | Clarification |
From above-mentioned result of the test as can be seen: Herba Erigerontis extract is more stable in pH value is 6.5～7.0 scope.
The investigation of experimental example 3 freeze-dry process
1. the screening of caffolding agent kind
Whether caffolding agent adds and kind influences the molding of freeze-dried powder, so at first this is screened.Get the medicinal liquid that contains crude drug 4.5g/ml, respectively with the solution mix homogeneously of caffolding agent mannitol, glucose and lactose, with 0.22 μ m membrane filtration postlyophilization, every XiLin bottle-packaging solution 3ml.
Lyophilisation condition: pre-freeze temperature-45 ℃, pre-freeze time 10h; The beginning evacuation, and be warming up to-40 ℃, keep 10h; Be warming up to-35 ℃ again, keep 6h; Be warming up to-20 ℃, keep 6h; Be warming up to-15 ℃, keep 5h; Be warming up to 0 ℃, keep 5h; Be warming up to 10 ℃, keep 4h; Be warming up to 20 ℃, keep 2h; Be warming up to 30 ℃, keep 2h to get finished product.
The screening of caffolding agent kind
| The caffolding agent kind | The caffolding agent solution concentration | Medicinal liquid: caffolding agent (v: v) | The finished product appearance character |
| Mannitol | 150mg/ml | 1∶2 1∶1 2∶1 | Not molding of not molding of not molding of atrophy atrophy atrophy |
| Glucose | 150mg/ml | 1∶2 1∶1 2∶1 | The atrophy molding of partly subsiding |
| Lactose | 150mg/ml | 1∶2 1∶1 2∶1 | The part not molding of not molding of atrophy atrophy of subsiding |
| Blank medicinal liquid | 3ml | Color and luster is inhomogeneous, not molding of atrophy |
By Biao Kede, the lyophilizing medicinal liquid need add caffolding agent; Its herb liquid: caffolding agent is that 2: 1 glucose molding effect is better relatively, so caffolding agent is finally selected glucose for use.
2. lyophilization conditional filtering
The lyophilization conditional filtering
Experimental result shows: spray bottle, atrophy phenomenon, the finished product appearance character that condition I and former lyophilisation condition make and the equal conformance with standard of moisture appear in condition II gained sample.Consider the practical situation of production, the condition I that the finally selected overall time spent is short, i.e. lyophilization condition is: pre-freeze temperature-45 ℃, pre-freeze time 10h; The beginning evacuation, and be warming up to-40 ℃, keep 10h; Be warming up to-35 ℃ again, keep 6h; Be warming up to-20 ℃, keep 6h; Be warming up to-15 ℃, keep 5h; Be warming up to 0 ℃, keep 5h; Be warming up to 10 ℃, keep 4h; Be warming up to 20 ℃, keep 2h; Be warming up to 30 ℃, keep 2h to get finished product.
Concrete embodiment:
Embodiment 1: 50 parts of Herba Erigerontiss, 30 parts of Radix Angelicae Sinensis, 20 parts of Radix Notoginseng
Get Herba Erigerontis, be ground into the coarse powder of 10 mesh sieves, add 8 times of volume 70% alcohol reflux 3 times, each 1 hour, merge extractive liquid,, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, the water heating for dissolving that adds 3 times of medical material volumes, filter, filtrate is crossed ZTC-1 type macroporous adsorbent resin, with 5 times of resinite hydrops and 3 times of resin volume 5% alcohol flushing impurity, use 30% alcohol desorption of 4 times of resin volumes then, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, Herba Erigerontis extract; Get Radix Notoginseng, be ground into the coarse powder of 10 mesh sieves, add 8 times of volume 70% alcohol reflux 3 times, each 1 hour, merge extractive liquid,, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, the water dissolution that adds 3 times of medical material volumes, filter, filtrate is crossed ZTC-1 type macroporous adsorbent resin, with 4 times of resinite hydrops and 3 times of resin volume 15% alcohol flushing impurity, use 60% alcohol desorption of 4 times of resin volumes then, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, Radix Notoginseng extract; Get Radix Angelicae Sinensis, be ground into the coarse powder of 10 mesh sieves, adding 8 times of decoctings boils 3 times, collecting decoction, filter, measuring relative density when filtrate is concentrated into 60 ℃ is 1.05～1.15, adds ethanol and makes and contain the alcohol amount and reach 70%, cold preservation is spent the night, filter, measuring relative density during filtrate recycling ethanol to 60 ℃ is 1.05～1.15, adds the water dissolution of 2 times of medical material bulking values, filter, filtrate is crossed the ZTC-1 macroporous adsorptive resins, with 5 times of resinite hydrops flushing resin columns, uses 4 times of resin volume 25% alcohol desorptions then, collect 25% ethanolysis imbibition, reclaim ethanol and get Radix Angelicae Sinensis extract 1; With the precipitation behind the precipitate with ethanol, add 3 times of amount waters for injection again, stir evenly, filter, filtrate concentrate Radix Angelicae Sinensis extract 2, above-mentioned Herba Erigerontis extract is added an amount of water for injection, heating, the sodium hydroxide solution with 40%, adjusting pH value is 6.5～7.0, make dissolving, add above-mentioned Radix Notoginseng extract, Radix Angelicae Sinensis extract 1 and 2 and the glucose mixing adds water for injection, add 0.3% active carbon again, heated and boiled keeps little and boiled 15 minutes, cooling, filter, filtrate adds to the full amount of water for injection, packing, lyophilization, promptly get freeze-dried powder injection, intravenous injection, once-a-day, each 1.
Embodiment 2: 50 parts of Herba Erigerontiss, 30 parts of Radix Angelicae Sinensis, 20 parts of Radix Notoginseng
Get Herba Erigerontis, be ground into the coarse powder of 10 mesh sieves, add 8 times of volume 70% alcohol reflux 3 times, each 1 hour, merge extractive liquid,, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, the water heating for dissolving that adds 3 times of medical material volumes, filter, filtrate is crossed ZTC-1 type macroporous adsorbent resin, with 5 times of resinite hydrops and 3 times of resin volume 5% alcohol flushing impurity, use 30% alcohol desorption of 4 times of resin volumes then, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, Herba Erigerontis extract; Get Radix Notoginseng, be ground into the coarse powder of 10 mesh sieves, add 8 times of volume 70% alcohol reflux 3 times, each 1 hour, merge extractive liquid,, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, the water dissolution that adds 3 times of medical material volumes, filter, filtrate is crossed ZTC-1 type macroporous adsorbent resin, with 4 times of resinite hydrops and 3 times of resin volume 15% alcohol flushing impurity, use 60% alcohol desorption of 4 times of resin volumes then, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, Radix Notoginseng extract; Get Radix Angelicae Sinensis, be ground into the coarse powder of 10 mesh sieves, adding 8 times of decoctings boils 3 times, collecting decoction, filter, measuring relative density when filtrate is concentrated into 60 ℃ is 1.05～1.15, adds ethanol and makes and contain the alcohol amount and reach 70%, cold preservation is spent the night, filter, measuring relative density during filtrate recycling ethanol to 60 ℃ is 1.05～1.15, adds the water dissolution of 2 times of medical material bulking values, filter, filtrate is crossed the ZTC-1 macroporous adsorptive resins, with 5 times of resinite hydrops flushing resin columns, uses 4 times of resin volume 25% alcohol desorptions then, collect 25% ethanolysis imbibition, reclaim ethanol and get Radix Angelicae Sinensis extract 1; Again with the precipitation behind the precipitate with ethanol, add 3 times of amount waters for injection, stir evenly, filter, filtrate concentrate Radix Angelicae Sinensis extract 2, above-mentioned Herba Erigerontis extract is added an amount of water for injection, heating, sodium hydroxide solution with 40%, adjusting pH value is 6.5～7.0, make dissolving, add above-mentioned Radix Notoginseng extract, Radix Angelicae Sinensis extract 1 and 2 and the glucose mixing adds water for injection, add 0.3% active carbon again, heated and boiled keeps little and boiled 15 minutes, cooling, coarse filtration, fine straining, add the injection water, divide to install to pacify and cut open bottle, seal sterilization and promptly get this product injection with small volume or concentrated solution for injection.
Embodiment 3: 50 parts of Herba Erigerontiss, 30 parts of Radix Angelicae Sinensis, 20 parts of Radix Notoginseng
Get Herba Erigerontis, be ground into the coarse powder of 10 mesh sieves, add 8 times of volume 70% alcohol reflux 3 times, each 1 hour, merge extractive liquid,, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, the water heating for dissolving that adds 3 times of medical material volumes, filter, filtrate is crossed ZTC-1 type macroporous adsorbent resin, with 5 times of resinite hydrops and 3 times of resin volume 5% alcohol flushing impurity, use 30% alcohol desorption of 4 times of resin volumes then, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, Herba Erigerontis extract; Get Radix Notoginseng, be ground into the coarse powder of 10 mesh sieves, add 8 times of volume 70% alcohol reflux 3 times, each 1 hour, merge extractive liquid,, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, the water dissolution that adds 3 times of medical material volumes, filter, filtrate is crossed ZTC-1 type macroporous adsorbent resin, with 4 times of resinite hydrops and 3 times of resin volume 15% alcohol flushing impurity, use 60% alcohol desorption of 4 times of resin volumes then, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, Radix Notoginseng extract; Get Radix Angelicae Sinensis, be ground into the coarse powder of 10 mesh sieves, adding 8 times of decoctings boils 3 times, collecting decoction, filter, measuring relative density when filtrate is concentrated into 60 ℃ is 1.05～1.15, adds ethanol and makes and contain the alcohol amount and reach 70%, cold preservation is spent the night, filter, measuring relative density during filtrate recycling ethanol to 60 ℃ is 1.05～1.15, adds the water dissolution of 2 times of medical material bulking values, filter, filtrate is crossed the ZTC-1 macroporous adsorptive resins, with 5 times of resinite hydrops flushing resin columns, uses 4 times of resin volume 25% alcohol desorptions then, collect 25% ethanolysis imbibition, reclaim ethanol and get Radix Angelicae Sinensis extract 1; Again with the precipitation behind the precipitate with ethanol, add 3 times of amount waters for injection, stir evenly, filter, filtrate concentrate Radix Angelicae Sinensis extract 2, above-mentioned Herba Erigerontis extract is added an amount of water for injection, heating, the sodium hydroxide solution with 40%, adjusting pH value is 6.5～7.0, make dissolving, add above-mentioned Radix Notoginseng extract, Radix Angelicae Sinensis extract 1 and 2 and the glucose mixing adds water for injection, adds 0.3% active carbon again, heated and boiled, keep little and boiled 15 minutes, cooling, coarse filtration, fine straining, add in an amount of the process glucose solution or sodium chloride solution that boil, promptly get high-capacity injection with the water for injection dilution.
Embodiment 4: 20 parts of Herba Erigerontiss, 10 parts of Radix Angelicae Sinensis
Get Herba Erigerontis, be ground into the coarse powder of 10 mesh sieves, added 6 times of volume 50% alcohol reflux 0.5 hour, merge extractive liquid,, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, the water heating for dissolving that adds 1 times of medical material volume, filter, filtrate is crossed ZTC-1 type macroporous adsorbent resin, with 2～7 times of resinite hydrops and 1 times of resin volume 5% alcohol flushing impurity, use 30% alcohol desorption of 2 times of resin volumes then, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, Herba Erigerontis extract; Get Radix Notoginseng, be ground into the coarse powder of 10 mesh sieves, added 6 times of volume 50% alcohol reflux 0.5 hour, merge extractive liquid,, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, the water dissolution that adds 1 times of medical material volume, filter, filtrate is crossed ZTC-1 type macroporous adsorbent resin, with 2～8 times of resinite hydrops and 1 times of resin volume 15% alcohol flushing impurity, use 60% alcohol desorption of 2 times of resin volumes then, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, Radix Notoginseng extract; Get Radix Angelicae Sinensis, be ground into the coarse powder of 10 mesh sieves, add 6 times of decoctings and boil 1 time, collecting decoction filters, and measuring relative density when filtrate is concentrated into 60 ℃ is 1.05～1.15, add ethanol and make and contain the alcohol amount and reach 50%, cold preservation is spent the night, and filters, measuring relative density during filtrate recycling ethanol to 60 ℃ is 1.05～1.15, the water dissolution that adds 1～3 times of medical material bulking value filters, and filtrate is crossed the ZTC-1 macroporous adsorptive resins, with 3 times of resinite hydrops flushing resin columns, use 2 times of resin volume 25% alcohol desorptions then, collect 25% ethanolysis imbibition, reclaim ethanol and get Radix Angelicae Sinensis extract 1, again with the precipitation behind the precipitate with ethanol, add 1 times of amount water for injection, stir evenly, filter, filtrate concentrate Radix Angelicae Sinensis extract 2, merge above-mentioned Herba Erigerontis extract, Radix Notoginseng extract, Radix Angelicae Sinensis extract 1 and 2, drying is pulverized, granulate, promptly get granule.
Embodiment 5: 80 parts of Herba Erigerontiss, 70 parts of Radix Angelicae Sinensis, Radix Notoginseng 60
Get Herba Erigerontis, be ground into the coarse powder of 10 mesh sieves, add 12 times of volume 80% alcohol reflux 4 times, each 3 hours, merge extractive liquid,, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, the water heating for dissolving that adds 4 times of medical material volumes, filter, filtrate is crossed ZTC-1 type macroporous adsorbent resin, with 7 times of resinite hydrops and 4 times of resin volume 5% alcohol flushing impurity, use 30% alcohol desorption of 8 times of resin volumes then, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, Herba Erigerontis extract; Get Radix Notoginseng, be ground into the coarse powder of 10 mesh sieves, add 12 times of volume 80% alcohol reflux 4 times, each 3 hours, merge extractive liquid,, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, the water dissolution that adds 4 times of medical material volumes, filter, filtrate is crossed ZTC-1 type macroporous adsorbent resin, with 8 times of resinite hydrops and 4 times of resin volume 15% alcohol flushing impurity, use 60% alcohol desorption of 8 times of resin volumes then, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, Radix Notoginseng extract; Get Radix Angelicae Sinensis, be ground into the coarse powder of 10 mesh sieves, add 12 times of decoctings and boil 4 times, collecting decoction filters, and measuring relative density when filtrate is concentrated into 60 ℃ is 1.05～1.15, add ethanol and make and contain the alcohol amount and reach 80%, cold preservation is spent the night, and filters, measuring relative density during filtrate recycling ethanol to 60 ℃ is 1.05～1.15, adds the water dissolution of 3 times of medical material bulking values, filters, filtrate is crossed the ZTC-1 macroporous adsorptive resins, with 8 times of resinite hydrops flushing resin columns, use 6 times of resin volume 25% alcohol desorptions then, collect 25% ethanolysis imbibition, reclaim ethanol and get Radix Angelicae Sinensis extract 1, with the precipitation behind the precipitate with ethanol, add 4 times of amount waters for injection again, stir evenly, filter, filtrate concentrate Radix Angelicae Sinensis extract 2, merge above-mentioned Herba Erigerontis extract, Radix Notoginseng extract, Radix Angelicae Sinensis extract 1 and 2, drying, pulverize, granulate, encapsulated, promptly get capsule.
Embodiment 6: 99 parts of 1 part of Herba Erigerontis and Radix Angelicae Sinensis
Get Herba Erigerontis, ethanol extraction, the extracting solution decompression recycling ethanol, refining, get Herba Erigerontis extract; Get Radix Notoginseng, ethanol extraction, the extracting solution decompression recycling ethanol, refining, get Radix Notoginseng extract; Get Radix Angelicae Sinensis, decocting boils, and collecting decoction filters, and filtrate concentrates refining, gets Radix Angelicae Sinensis extract, merges above-mentioned each extract, adds distilled water, promptly gets oral liquid.
Embodiment 7: 1 part of 99 parts of Herba Erigerontiss and Radix Angelicae Sinensis
Get Herba Erigerontis, ethanol extraction, the extracting solution decompression recycling ethanol, refining, get Herba Erigerontis extract; Get Radix Notoginseng, ethanol extraction, the extracting solution decompression recycling ethanol, refining, get Radix Notoginseng extract; Get Radix Angelicae Sinensis, decocting boils, and collecting decoction filters, and filtrate concentrates refining, gets Radix Angelicae Sinensis extract, merges above-mentioned each extract, and pill promptly gets pill.
Embodiment 8: 99 parts of Herba Erigerontis extracts, 1 part of Radix Angelicae Sinensis extract
Get Herba Erigerontis, ethanol extraction, the extracting solution decompression recycling ethanol, refining, get Herba Erigerontis extract; Get Radix Notoginseng, ethanol extraction, the extracting solution decompression recycling ethanol, refining, get Radix Notoginseng extract; Get Radix Angelicae Sinensis, decocting boils, and collecting decoction filters, and filtrate concentrates refining, gets Radix Angelicae Sinensis extract, merges above-mentioned each extract, pulverizes, and promptly gets powder.
Embodiment 9: 1 part of Herba Erigerontis extract, 99 parts of Radix Angelicae Sinensis extracts
Get Herba Erigerontis extract, Radix Angelicae Sinensis extract, merge above-mentioned each extract, add carrageenan, promptly get gel.
Embodiment 10: 80 parts of Herba Erigerontis extracts, 70 parts of Radix Angelicae Sinensis extracts, Radix Notoginseng extract 60
Get Herba Erigerontis extract, Radix Angelicae Sinensis extract, merge above-mentioned each extract, the extruding pill promptly gets pellet.
Embodiment 11: 20 parts of Herba Erigerontis extracts, 10 parts of Radix Angelicae Sinensis extracts
Get Herba Erigerontis extract, Radix Angelicae Sinensis extract, merge above-mentioned each extract, pulverize, granulate, tabletting promptly gets tablet.
Embodiment 12: 50 parts of Herba Erigerontis extracts, 30 parts of Radix Angelicae Sinensis extracts, Radix Notoginseng extract 20
Get Herba Erigerontis extract, Radix Angelicae Sinensis extract, merge above-mentioned each extract, granulate, promptly get granule.
Embodiment 13: 99 parts of 1 part of Herba Erigerontis, Radix Angelicae Sinensis and Radix Notoginseng
Get Herba Erigerontis, ethanol extraction, the extracting solution decompression recycling ethanol, refining, get Herba Erigerontis extract; Get Radix Notoginseng, ethanol extraction, the extracting solution decompression recycling ethanol, refining, get Radix Notoginseng extract; Get Radix Angelicae Sinensis, decocting boils, and collecting decoction filters, and filtrate concentrates refining, gets Radix Angelicae Sinensis extract, merges above-mentioned each extract, pulverizes, and granulates, and promptly gets granule.
Embodiment 14: 1 part of 99 parts of Herba Erigerontiss, Radix Angelicae Sinensis and Radix Notoginseng
Get Herba Erigerontis, ethanol extraction, the extracting solution decompression recycling ethanol, refining, get Herba Erigerontis extract; Get Radix Notoginseng, ethanol extraction, the extracting solution decompression recycling ethanol, refining, get Radix Notoginseng extract; Get Radix Angelicae Sinensis, decocting boils, and collecting decoction filters, and filtrate concentrates refining, gets Radix Angelicae Sinensis extract, merges above-mentioned each extract, pulverizes, and granulates, and tabletting promptly gets tablet.
Embodiment 15: 99 parts of Herba Erigerontis effective sites, 1 part of Radix Angelicae Sinensis effective site
Get Herba Erigerontis, Radix Angelicae Sinensis effective site, mixing adds distilled water, promptly gets oral liquid.
Embodiment 16: 1 part of Herba Erigerontis effective site, 99 parts of Radix Angelicae Sinensis effective sites
Get Herba Erigerontis effective site, Radix Angelicae Sinensis effective site, mixing adds carrageenan, promptly gets gel.
Embodiment 17: 80 parts of Herba Erigerontis effective sites, 70 parts of Radix Angelicae Sinensis effective sites, 60 parts of Sanchi effective-components
Get Herba Erigerontis effective site, Radix Angelicae Sinensis effective site, Sanchi effective-component, mixing, the extruding pill promptly gets pellet.
Embodiment 18: 20 parts of Herba Erigerontis effective sites, 10 parts of Radix Angelicae Sinensis effective sites
Get Herba Erigerontis effective site, Radix Angelicae Sinensis effective site, mixing is pulverized, and granulates, and tabletting promptly gets tablet.
Embodiment 19: 50 parts of Herba Erigerontis effective sites, 30 parts of Radix Angelicae Sinensis effective sites, 20 parts of Sanchi effective-components
Get Herba Erigerontis effective site, Radix Angelicae Sinensis effective site, Sanchi effective-component, mixing is granulated, and promptly gets granule.
Embodiment 20: 50 parts of Herba Erigerontis effective sites, 30 parts of Radix Angelicae Sinensis effective sites, 20 parts of Sanchi effective-components
Get Herba Erigerontis effective site, Radix Angelicae Sinensis effective site, Sanchi effective-component, mixing, with the polyoxyethylene monostearate is substrate, and the proportioning of medicine and substrate is 1: 1.5, mixing, splash in the coolant methyl-silicone oil, dripping the system footpath is the dropper of 6.1mm, and 20～30/min splashes in the long cooling column of 120cm, adopts the gradient cooling, the Temperature Distribution of gradient liquid coolant is 40 ℃～50 ℃, 20 ℃～30 ℃, 0 ℃～4 ℃, promptly gets drop pill.
Embodiment 21: 50 parts of Herba Erigerontis extracts, 30 parts of Radix Angelicae Sinensis extracts, 20 parts of Radix Notoginseng extracts
Get Herba Erigerontis extract, Radix Angelicae Sinensis extract, Radix Notoginseng extract, mixing, with the polyoxyethylene monostearate is substrate, and the proportioning of medicine and substrate is 1: 1.5, mixing, splash in the coolant methyl-silicone oil, dripping the system footpath is the dropper of 6.1mm, and 20～30/min splashes in the long cooling column of 120cm, adopts the gradient cooling, the Temperature Distribution of gradient liquid coolant is 40 ℃～50 ℃, 20 ℃～30 ℃, 0 ℃～4 ℃, promptly gets drop pill.
Embodiment 22: 50 parts of Herba Erigerontiss, 30 parts of Radix Angelicae Sinensis, 20 parts of Radix Notoginseng
Get Herba Erigerontis, be ground into the coarse powder of 10 mesh sieves, add 12 times of volume 80% alcohol reflux 4 times, each 3 hours, merge extractive liquid,, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, the water heating for dissolving that adds 4 times of medical material volumes, filter, filtrate is crossed ZTC-1 type macroporous adsorbent resin, with 7 times of resinite hydrops and 4 times of resin volume 5% alcohol flushing impurity, use 30% alcohol desorption of 8 times of resin volumes then, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, Herba Erigerontis extract; Get Radix Notoginseng, be ground into the coarse powder of 10 mesh sieves, add 12 times of volume 80% alcohol reflux 4 times, each 3 hours, merge extractive liquid,, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, the water dissolution that adds 4 times of medical material volumes, filter, filtrate is crossed ZTC-1 type macroporous adsorbent resin, with 8 times of resinite hydrops and 4 times of resin volume 15% alcohol flushing impurity, use 60% alcohol desorption of 8 times of resin volumes then, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, Radix Notoginseng extract; Get Radix Angelicae Sinensis, be ground into the coarse powder of 10 mesh sieves, adding 12 times of decoctings boils 4 times, collecting decoction, filter, measuring relative density when filtrate is concentrated into 60 ℃ is 1.05～1.15, adding ethanol makes and contains the alcohol amount and reach 80%, cold preservation is spent the night, filter, measuring relative density during filtrate recycling ethanol to 60 ℃ is 1.05～1.15, adds the water dissolution of 3 times of medical material bulking values, filter, filtrate is crossed the ZTC-1 macroporous adsorptive resins, with 8 times of resinite hydrops flushing resin columns, uses 6 times of resin volume 25% alcohol desorptions then, collect 25% ethanolysis imbibition, reclaim ethanol and get Radix Angelicae Sinensis extract 1,, add 4 times of amount waters for injection again with the precipitation behind the precipitate with ethanol, stir evenly, filter, filtrate concentrate Radix Angelicae Sinensis extract 2, merge above-mentioned Herba Erigerontis extract, Radix Notoginseng extract, Radix Angelicae Sinensis extract 1 and 2, mixing, with the polyoxyethylene monostearate is substrate, and the proportioning of medicine and substrate is 1: 1.5, mixing, splash in the coolant methyl-silicone oil, dripping the system footpath is the dropper of 6.1mm, and 20～30/min splashes in the long cooling column of 120cm, adopts the gradient cooling, the Temperature Distribution of gradient liquid coolant is 40 ℃～50 ℃, 20 ℃～30 ℃, 0 ℃～4 ℃, promptly get drop pill.
Extract of the present invention can be commercially available or be made by the inventive method, wherein contains scutellarin in the Herba Erigerontis extract and is not less than 3%, contains ferulic acid in the Radix Angelicae Sinensis extract and is not less than 0.5%, contains arasaponin R1, ginsenoside Rb in the Radix Notoginseng extract
1And ginsenoside Rg
1Total amount be not less than 3%.
Effective site of the present invention can be commercially available or be made by the inventive method, wherein contain flavones ingredient in the effective site Herba Erigerontis total flavones of Herba Erigerontis and be not less than 50%, contain saponin component in the effective site Radix Notoginseng total arasaponins of Radix Notoginseng and be not less than 50%, contain organic acids composition in the effective site Radix Angelicae Sinensis total acid of Radix Angelicae Sinensis and be not less than 50%.
Claims (10)
1. Chinese medicine preparation for the treatment of cardiovascular and cerebrovascular disease is characterized in that: calculate according to components by weight percent, it by 99～1 parts of 1～99 part of Herba Erigerontis and Radix Angelicae Sinensis through extracting refining forming; Or be made with Radix Angelicae Sinensis extract or the effective site that corresponding weight portion medical material obtains after extraction by Herba Erigerontis extract or the effective site that corresponding weight portion medical material obtains after extracting.
2. according to the Chinese medicine preparation of the described treatment cardiovascular and cerebrovascular disease of claim 1, it is characterized in that: calculate according to components by weight percent, it by 99～1 parts of 1～99 part of Herba Erigerontis, Radix Angelicae Sinensis and Radix Notoginseng through extracting refining forming; Or the extract or the effective site that are obtained after extracting by corresponding weight portion medical material are made.
3. according to the Chinese medicine preparation of the described treatment cardiovascular and cerebrovascular disease of claim 2, it is characterized in that: calculate according to parts by weight, it is made for 0～60 part by 20～80 parts of Herba Erigerontiss, 10～70 parts of Radix Angelicae Sinensis, Radix Notoginseng, or the extract or the effective site that are obtained after extracting by corresponding weight portion medical material are made.
4. according to the Chinese medicine preparation of the described treatment cardiovascular and cerebrovascular disease of claim 3, it is characterized in that: calculate according to parts by weight, it is made for 20 parts by 50 parts of Herba Erigerontiss, 30 parts of Radix Angelicae Sinensis, Radix Notoginseng, or the extract or the effective site that are obtained after extracting by corresponding weight portion medical material are made.
5. according to the Chinese medicine preparation of any described treatment cardiovascular and cerebrovascular disease among the claim 1-4, it is characterized in that: contain scutellarin in the Herba Erigerontis extract and be not less than 3%, contain ferulic acid in the Radix Angelicae Sinensis extract and be not less than 0.5%, the total amount that contains arasaponin R1, ginsenoside Rb1 and ginsenoside Rg1 in the Radix Notoginseng extract is not less than 3%.
6. according to the Chinese medicine preparation of any described treatment cardiovascular and cerebrovascular disease among the claim 1-4, it is characterized in that: contain flavones ingredient in the effective site Herba Erigerontis total flavones of Herba Erigerontis and be not less than 50%, contain saponin component in the effective site Radix Notoginseng total arasaponins of Radix Notoginseng and be not less than 50%, contain organic acids composition in the effective site Radix Angelicae Sinensis total acid of Radix Angelicae Sinensis and be not less than 50%.
7. according to the Chinese medicine preparation of any described treatment cardiovascular and cerebrovascular disease among the claim 1-4, it is characterized in that: described preparation is injection and oral formulations; Injection comprises: be directly used in the injection of drug administration by injection, directly supply the venous transfusion of intravenous drip, need be used for the concentrated solution for injection of intravenous drip and injectable sterile powder and the aseptic block that makes with freeze-drying or spray drying method after diluting; Oral formulations comprises: all acceptable dosage forms on the pharmaceuticss such as tablet, dispersible tablet, effervescent tablet, oral cavity disintegration tablet, capsule, soft capsule, microcapsule, granule, pill, powder, drop pill, slow releasing preparation, controlled release preparation, oral liquid, soft extract, extractum, gel, membrane.
8. as the preparation method of the Chinese medicine preparation of any described treatment cardiovascular and cerebrovascular disease among the claim 1-7, it is characterized in that: get Herba Erigerontis, ethanol extraction, the extracting solution decompression recycling ethanol, adopt in column chromatography, extraction, the sedimentation method one or more to make with extra care on this basis, Herba Erigerontis extract or effective site; Get Radix Notoginseng, ethanol extraction, the extracting solution decompression recycling ethanol adopts in column chromatography, extraction, the sedimentation method one or more to make with extra care on this basis, Radix Notoginseng extract or effective site; Get Radix Angelicae Sinensis, decocting boils, collecting decoction, filter, filtrate concentrates, and adopts in column chromatography, extraction, the sedimentation method one or more to make with extra care on this basis, get Radix Angelicae Sinensis extract or effective site, merge above-mentioned each extract or effective site, make different preparations.
9. according to the preparation method of the Chinese medicine preparation of the described treatment cardiovascular and cerebrovascular disease of claim 8, it is characterized in that: get Herba Erigerontis, be ground into the coarse powder of 10 mesh sieves, add 6～12 times of volume 50～80% alcohol reflux 1～4 time, each 0.5～3 hour, merge extractive liquid,, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, the water heating for dissolving that adds 1～4 times of medical material volume, filter, filtrate is crossed resin column, with 2～7 times of resinite hydrops and 1～4 times of resin volume 5% alcohol flushing impurity, use 30% alcohol desorption of 2～8 times of resin volumes then, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, Herba Erigerontis extract; Get Radix Notoginseng, be ground into the coarse powder of 10 mesh sieves, add 6～12 times of volume 50～80% alcohol reflux 1～4 time, each 0.5～3 hour, merge extractive liquid,, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, the water dissolution that adds 1～4 times of medical material volume, filter, filtrate is crossed resin column, with 2～8 times of resinite hydrops and 1～4 times of resin volume 15% alcohol flushing impurity, use 60% alcohol desorption of 2～8 times of resin volumes then, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, Radix Notoginseng extract; Get Radix Angelicae Sinensis, be ground into the coarse powder of 10 mesh sieves, and added 6～12 times of decoctings and boil collecting decoction 1～4 time, filter, measuring relative density when filtrate is concentrated into 60 ℃ is 1.05～1.15, adds ethanol and makes and contain the alcohol amount and reach 50～80%, and cold preservation is spent the night, filter, measuring relative density during filtrate recycling ethanol to 60 ℃ is 1.05～1.15, adds the water dissolution of 1～3 times of medical material bulking value, filters, filtrate is crossed resin column, with 3～8 times of resinite hydrops flushing resin columns, use 2 ~ 6 times of resin volume 25% alcohol desorptions then, collect 25% ethanolysis imbibition, reclaim ethanol and get Radix Angelicae Sinensis extract 1, with the precipitation behind the precipitate with ethanol, add 1～4 times of amount water for injection again, stir evenly, filter, filtrate concentrate Radix Angelicae Sinensis extract 2, merge above-mentioned Herba Erigerontis extract, Radix Notoginseng extract, Radix Angelicae Sinensis extract 1 and 2 is made different preparations.
10. according to the preparation method of the Chinese medicine preparation of the described treatment cardiovascular and cerebrovascular disease of claim 9, it is characterized in that: the freeze-dried powder injection in the described preparation prepares like this: get Herba Erigerontis, be ground into the coarse powder of 10 mesh sieves, add 8 times of volume 70% alcohol reflux 3 times, each 1 hour, merge extractive liquid,, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, the water heating for dissolving that adds 3 times of medical material volumes, filter, filtrate is crossed ZTC-1 type macroporous adsorbent resin, with 5 times of resinite hydrops and 3 times of resin volume 5% alcohol flushing impurity, use 30% alcohol desorption of 4 times of resin volumes then, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, Herba Erigerontis extract; Get Radix Notoginseng, be ground into the coarse powder of 10 mesh sieves, add 8 times of volume 70% alcohol reflux 3 times, each 1 hour, merge extractive liquid,, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, the water dissolution that adds 3 times of medical material volumes, filter, filtrate is crossed ZTC-1 type macroporous adsorbent resin, with 4 times of resinite hydrops and 3 times of resin volume 15% alcohol flushing impurity, use 60% alcohol desorption of 4 times of resin volumes then, collect stripping liquid, measuring relative density during decompression recycling ethanol to 60 ℃ is 1.05～1.15, Radix Notoginseng extract; Get Radix Angelicae Sinensis, be ground into the coarse powder of 10 mesh sieves, adding 8 times of decoctings boils 3 times, collecting decoction, filter, measuring relative density when filtrate is concentrated into 60 ℃ is 1.05～1.15, adds ethanol and makes and contain the alcohol amount and reach 70%, cold preservation is spent the night, filter, measuring relative density during filtrate recycling ethanol to 60 ℃ is 1.05～1.15, adds the water dissolution of 2 times of medical material bulking values, filter, filtrate is crossed the ZTC-1 macroporous adsorptive resins, with 5 times of resinite hydrops flushing resin columns, uses 4 times of resin volume 25% alcohol desorptions then, collect 25% ethanolysis imbibition, reclaim ethanol and get Radix Angelicae Sinensis extract 1; With the precipitation behind the precipitate with ethanol, add 3 times of amount waters for injection again, stir evenly, filter, filtrate concentrate Radix Angelicae Sinensis extract 2, above-mentioned Herba Erigerontis extract is added an amount of water for injection, heating, sodium hydroxide solution with 40%, adjusting pH value is 6.5～7.0, makes dissolving, adds above-mentioned Radix Notoginseng extract, Radix Angelicae Sinensis extract 1 and 2 and the glucose mixing, add water for injection, add 0.3% active carbon again, heated and boiled keeps little and boiled 15 minutes, cooling, filter, filtrate adds to the full amount of water for injection, packing, lyophilization, promptly.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102119960A (en) * | 2010-01-07 | 2011-07-13 | 大道隆达（北京）医药科技发展有限公司 | Medicinal composition for activating blood, dissolving stasis, tonifying qi and freeing vessels |
| CN105878569A (en) * | 2014-12-04 | 2016-08-24 | 杜泽均 | Powder for tonifying kidneys, removing stasis and dredging collaterals |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102119960A (en) * | 2010-01-07 | 2011-07-13 | 大道隆达（北京）医药科技发展有限公司 | Medicinal composition for activating blood, dissolving stasis, tonifying qi and freeing vessels |
| CN105878569A (en) * | 2014-12-04 | 2016-08-24 | 杜泽均 | Powder for tonifying kidneys, removing stasis and dredging collaterals |
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