CN1733000A - Ceratopteris richardii extract and its application in preparation of anti-tumor formulation - Google Patents
Ceratopteris richardii extract and its application in preparation of anti-tumor formulation Download PDFInfo
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Abstract
The invention provides a parkeriaceae whose preparing process comprises, washing dried parkeriaceae, loading into an extraction tank, heating and collecting the first extract, charging distilled water, heating and collecting the second and third extracts, merging the extracts, filtering and stewing under room temperature, finally concentrating the filtration solution to extract. The invention further provides the use of the extract in preparing antineoplastic preparations.
Description
Technical field
The invention belongs to tcm field, effective preparation of a kind of specifically antitumor Chinese medicine and the application in pharmacy thereof, more particularly, it relates to Ceratopteris richardii extract and the application in the preparation anti-tumor agent thereof.
Background technology
Animal body inner cell division is regulated out of control and cell infinite multiplication is called tumor cell, i.e. cancerous cell.Normal hyperplasia is limited.Because self-control mechanism is destroyed, cancerous cell is hypertrophy without end.In an entity, need each member's coordination, any member's the unusual reduction that all may cause systematic function.Cancer is that cell growth, breeding are out of hand, makes due to tissue and organs abnormality hypertrophy, reduction or the forfeiture proper function.Cancer be body under the effect of various carcinogenic factors, local organization paraplasm and the neoplasm that forms.Cancer is the result of gene mutation often.Gene mutation is the approach and the inevitable mode of biological evolution.When environmental change, in order to conform, biology must be to self making corresponding finishing.Hypercorrect the time, just form cancer.
According to interrelated data statistics, the whole world because of the cancer mortality number in 1975 about 3,800,000,1980 be 4,000,000,1985 be 5,000,000,1997 be 6,240,000,2000 about 9,000,000, the year two thousand twenty may surpass 10,000,000 according to estimates.Preceding 5 cancers in the whole world are respectively: pulmonary carcinoma (98.7 ten thousand), gastric cancer (77.6 ten thousand), colorectal cancer (49.5 ten thousand), hepatocarcinoma (38.6 ten thousand) and breast carcinoma (37.6 ten thousand).In recent years because the pollution that interior decoration causes causes hematopathy patient number to increase severely.Can only take palliative treatment to above-mentioned various tumor treatment at present, or radiotherapy, chemotherapy and operative treatment.In two kinds of Therapeutic Method of pro-, can kill a certain amount of cancerous cell really, but in killing tumor cell, a large amount of normal cells also is subjected to serious damage, causes immunologic function and hemopoietic function to be destroyed, bring many side effect to treatment.
Apoptosis (apoptosis) is normal cell antagonism viral infection and the effective way of removing damaging cells, and this research has very high value to the treatment of human cancer, neuropathy, autoimmune disease, acquired immune deficiency syndrome (AIDS) etc.Apoptosis refers to a self-desttruction equipment of human body cell, and this device plays a part very important in life process; Self-desttruction equipment goes wrong, and will cause a lot of human diseasess.A lot of cancers are exactly because cell self-destruction process can't normally start, the more and more apoptosis that cause of cell number.Thereby apoptosis has the important biology and the meaning of medical science, has been subjected to widely to pay attention to, and has some idea of from Nobel physiology in 2002 and just issuing of medical science prize.Apoptosis is different from necrocytosis, and it is a body because specific needs and by the cell normality death of Gene Handling.When cell received apoptosis information, cell was crimped to many apoptotic bodies from complete cellular morphology, often was accompanied by nuclear concentrating, the degraded of DNA, but cracking can not take place in intracellular organelle, and cellular content can not leak outside, so apoptotic cell can not cause inflammation.Apoptotic body can be by contiguous cell or macrophage phagocytic, contained content is digested, absorbs enters circulation again, and apoptosis is not only brought in constant renewal in for the body tissue cell and provide the approach of a safety but also a kind of means of to be body remove specific cell in self growth course.So apoptosis claims it is to be programmed cell death, promptly be a kind of physiological of cell, " the conscious suicide " of initiative, these cells are died an death regular, seemingly undertaken by " program " finished, just as the withering and falling of sheet sheet leaves in autumn, so this cell death just is called " apoptosis ".Neonate, baby, child are to teenager, and in the stage before these a series of human development's maturations, the cell birth is many on the whole, and dead lacks, so health could be grown.After reaching maturity, human inner cell's birth and death are in a dynamic equilibrium stage, and all have last trillion cells to be born every day in the adult human body, and last trillion cells " programmed death " are arranged again simultaneously.In the body of health, the life and death of cell extremely always is in the benign dynamic equilibrium, if this balance is destroyed, the people will be ill.If cell that should death does not have death, just may cause the pernicious growth of cell, form cancer.If hypercellularity ground that should not be dead is dead, such as the attack that is subjected to HIV (human immunodeficiency virus) (Human Immunodeficiency Virus), should not the dead large quantities of death of lymphocyte, will destroy the immunocompetence of human body, cause similar acquired immune deficiency syndrome (AIDS) to be shown effect.Apoptotic notion, at first propose in 1972 by Kerr, some national scientists have begun to utilize the mechanism of " programmed cell death " at present, research can be treated the new method of multiple disease, and other scientists continue to inquire into apoptosis-related problem with the biological and chemical way.Nearly stage scientists begins to utilize the apoptotic mechanism of having understood to treat and apoptotic numerous disease, as autoimmune disease, AIDS, T cell depletion, neurasthenia, control cell proliferation and differentiation, the generation of tumor, the process of development.Especially aspect tumor treatment, produced a kind of and the diverse new method of traditional method---apoptosis therapy.For example the endometrial cell apoptosis, propagation unbalance may be one of pathogeny of adenomyosis.Reach the purpose that tumor is dwindled, disappeared by inducing apoptosis of tumour cell, become the research focus of current oncotherapy.At present the clinical research tumor type of suicide gene therapy mainly contains cerebral glioma, ovarian cancer, carcinoma of prostate, hepatocarcinoma, incidence squamous cell carcinoma, malignant mesothe, melanoma, colon cancer, nonsmall-cell lung cancer, cancer eye, carcinoma of penis etc., and the result all shows and utilizes suicide gene to carry out the effectiveness and the safety of oncotherapy.And for example at the finely regulating of cell quantity, the removing of potential danger cell and three aspects such as fetal development of organism, apoptosis plays a significant role.In concrete case, not only have hyperplasia as finding in the gastric cancer, also have apoptosis simultaneously, gastric cancer be apoptosis and hypertrophy result out of proportion.
The hope that the operation of tumor, radiotherapy, chemotherapy and interventional therapy have brought existence for numerous tumor patients, but up to now, tumor is still the mankind is endangered one of maximum disease.Through the research of nearly half a century, it is found that, the anticancer active constituent in the plant, as polysaccharose substance, can killing tumor cell, and normal cell had the certain protection effect.Recently studies show that Chinese medicine is inducing apoptosis of tumour cell effectively, for tumor patient has brought Gospel.
Apoptosis is a kind of spontaneous, the process of cell death initiatively that is prevalent in the multicellular organisms, is that organism is kept the metastable intrinsic mechanism of cell quantity, and this mechanism is unusual as if obstacle or generation are arranged, and just might cause tumor or other pathological changes.And tumor to be a kind of apoptosis very few and breed too much disease, if can suppress the propagation of tumor cell and induce its apoptosis, tumor cell just might stop growing, thereby the herb induction apoptosis of tumor cells has become the new way of a treatment tumor that gets a good chance of.At present the Chinese medicine of inducing apoptosis of tumour cell commonly used has: simple prescription preparation Scorpio infusion extract can be induced the HL-60 apoptosis, and the apoptosis ratio increases with drug level and rises.Rhizoma Amorphophalli is the inducing mouse apoptosis of tumor cells effectively.Rhizoma Bolbostematis all has the obvious suppression effect to the people's granular cell carcinoma of kidney of In vitro culture and the growth of brown rat transplantability people granular cell carcinoma of kidney.The domestic and international medical circle of compound Chinese medicinal preparation reports that successively decoction of ten powerful tonics, Liujunzi Tang, Radix Ginseng skin beautifying soup, Herba Sidae Rhombifoliae soup, Radix Angelicae Sinensis decoction for tonifying blood etc. all have the effect of inducing different apoptosis of tumor cells.The Japan scholar is the most careful to the research of Herba Sidae Rhombifoliae soup, they adopt saikoside, ginsenoside, glycyrrhizin, Radix Scutellariae Saponin and the whole compositions of water solublity thereof of Herba Sidae Rhombifoliae soup, handle hepatoma cell strain, cholangiocarcinoma cell strain, normal rat hepatocyte and normal person's periphery lymphocyte of In vitro culture respectively.Found that each active ingredient uses very low to the apoptosis induction rate of JEG-3 separately, and the effect of Herba Sidae Rhombifoliae soup whole part is the most obvious, and concentration dependent is arranged, and all reagents all do not have influence to normal cell.
This experiment shows that Chinese medicine compound is more effective than the Chinese medicine extraction composition aspect inducing apoptosis of tumour cell.
It is the anticarcinogen that extracts from the natural plants Ramulus et folium taxi cuspidatae that Chinese medicine effectively extracts the composition paclitaxel, can induce the apoptosis of people's acute promyelocytic leukemia, human breast cancer cell, human esophagus cancer cell.Elemene is the effective ingredient that extracts from the Chinese medicine Rhizoma Curcumae, has broad-spectrum anti-tumor activity, can induce human leukemia cell and people's hepatic ascites apoptosis of tumor, and obviously influences the division cycle of tumor cell.Camptothecine is the effective ingredient that extracts from the Chinese medicine Fructus seu radix camptothecae acuminatae (Fructus Camptothecae Acuminatae), but inducing leukemia cell, human pancreatic cancer cell apoptosis.Venenum Bufonis is the decorating film that forms after drying from Bufo siccus secretions, except that having cardiotonic, also human leukemia cell line, colorectal carcinoma cell line is had apoptosis-induced effect.Venenum Bufonis has specificity to human cancer cell, to the almost not influence of cancerous cell of rat and mice.Quercetin, icariine, leaf autumn alkali, curcumin, Radix Sanguisorbae tannin, apigenin, trichosanthin, sinomenine, ursolic acid etc. all have apoptosis-induced effect to tumor cell.
A large amount of clinical practice and studies have shown that adopts Chinese medicine in conjunction with the chemotherapeutic treatment tumor, can obtain more simple one and adopt the better therapeutic effect of chemotherapy.Do like this, the one, can strengthen the kill capability of chemotherapeutics to cancerous cell, alleviate the toxicity of chemotherapy, chemotherapy is finished smoothly, and effectively improved the life quality and the survival rate of tumor patient.The 2nd, can protect and raise immunity, suppress tumor growth.First Ganoderma cancer therapy drug sunrecome of the whole world (former Chinese ganoderma extract) is " the accurate word of traditional Chinese medicines " Ganoderma anticancer drugs of the unique approval of China, is become first the Chinese Chinese medicine medicine for preventing that enters American market by the U.S..This is the anticancer maximum achievement of present Chinese medicine.
Chemotherapy can cause body's immunity low.Immunologic hypofunction is one of root of tumour progression, recurrence and transfer.The Chinese medicine of traditional Chinese medical science the body resistance strengthening and constitution consolidating (as the Radix Astragali, Radix Pseudostellariae, the Rhizoma Atractylodis Macrocephalae, Poria, Semen Coicis, Fructus Ligustri Lucidi, Fructus Lycii etc.) both can alleviate chemotherapeutic toxicity and untoward reaction symptom, hemopoietic, immunosuppressive action that again can the antagonism chemotherapy.In recent years, inducer remission-set upright treatment-after treatment-set upright the treatment pattern or the program of treatment has been widely used in the treatment of solid tumor.Treatment by Chinese herbs cancer, inducing apoptosis of tumour cell have advantages such as the little and difficult recurrence of toxic and side effects, in tumor operation, radiotherapy, chemotherapy and interventional therapy, principle according to tcm treatment according to syndrome differentiation, select relevant Chinese medicine to allocate in decoct or the compound recipe, can improve clinical efficacy, prolong the tumor patient life span, improve tumor patient quality of life.
The drug resistance of tumor cell has limited the therapeutic effect of chemicals on the other hand, is the main cause of chemotherapy failure.The drug resistance of tumor is meant the insensitivity of tumor to chemicals.According to statistics, it is relevant with drug resistance to accept the death of tumor patient of chemotherapy more than 90%.According to the drug resistance characteristics of tumor cell, drug resistance can be divided into primary resistance (PDR) and multidrug resistance (MDR) two big classes, and multidrug resistance is meant that tumor cell contacts the toleration that produces after a kind of cancer therapy drug multiple cancer therapy drug.Therefore, people begin to seek effective ingredient or compound recipe with reversion MDR from Chinese herbal medicine, and first meeting achievement.
Calcium channel blocking: some Chinese medicines have calcium channel blocking action, and Chinese scholar is subjected to the inspiration of the anti-multidrug resistance effect of calcium channel blocker verapamil, have done the research of a large amount of Chinese medicine reversion MDRs.Confirm the multidrug resistance that Chinese medicine calcium ion channel blocker ginsenoside monomer (Rb1) can reversing multi-medicine drug resistance of leukaemia cell line (K-562/HHT), and be dose dependent; Bisbenzylisoquinoline alkaloid is the Chinese medicine monomer with calcium channel blocking action, and there have MDR to reverse in experiment in vitro to be active, and its mechanism of action is similar to verapamil, and the film regulating action is all arranged.This research has also taken the lead in carrying out the research with the Chinese medicine reversal of multidrug resistance of tumor cells at home and abroad, and these Chinese medicines mainly contain Radix Salviae Miltiorrhizae, ligustrazine, Dauricine, paeonol, panaxoside Rg 3, elemene and derivant thereof, paclitaxel, Radix Paeoniae Rubra, Radix Et Rhizoma Rhei, Semen Persicae, Fructus Psoraleae, Realgar, arsenic trioxide, scorpion venom etc.Studies show that above-mentioned Chinese medicine has the effect of tangible reverse multiple drug resistance of tumor, wherein clinical toxic and side effects such as Radix Salviae Miltiorrhizae, ligustrazine, paeonol are little, can heavy dose ofly use clinical; Elemene, paclitaxel, Realgar etc. itself are exactly antineoplastic agent, clinically with other antineoplastic agent use in conjunction, both can bring into play the tumor effect of killing certainly of its body, have the reverse multiple drug resistance of tumor effect again concurrently.
This shows that in the R﹠D process of anticancer drugs, Chinese medicine is very potential research direction.Chinese medicine is controlled cancer for symptom that alleviates cancer patient and misery, improves life quality, prolongs life, reduces the mortality rate of cancer, and its important meaning is all arranged.
As everyone knows, the pteridophyta and the mankind's relation is very close, and China working people cures the disease with pteridophyta very early.Up to the present,, has kind more than 100 at least, as the climing Lycopodium clavatum energy of China fir wind-damp dispelling, relaxing muscles and tendons to promote blood circulation as medicinal fern; Herba Clinopodii Polycephali can be controlled suppurative osteomyelitis; Folium Stenolomatis can be controlled bacillary dysentery, acute enteritis, sore; The long handle Folium Pyrrosiae can be controlled acute and chronic nephritis, pyelonephritis etc.; Dryopteris crassirhizoma and its many allied species can be controlled the liver fluke disease of cattle and sheep; Spora Lygodii is controlled urinary tract infection, calculus of urethra; The Herba Selaginellae external application, it is hemorrhage to control knife injury; Herba Selaginellae Involventis is controlled diseases such as jaundice due to damp-heat, edema, haematemesis; Herba Botrychii is controlled infantile convulsion; The hard bone the kidney invigorating of osteomiosis complementary energy, promoting blood circulation and stopping pain; It is hemorrhage that the scale of Cibotium barometz (L.) J. Sm. can end knife injury; The Mongolian oak Herba pteridii latiusculi can be mended bone analgesia, dispelling the wind and dampness pathogens numbness; The root stock of Rhizoma Osmundae can be controlled diseases such as abdominal pain due to worm stagnation, influenza, also as the deinsectization pesticide, and the venom medicine or the like.The purposes of the Dai nationality tradition wild plant and introduce a fine variety the record Herba Ceratopteridis Thalictroidis can treating tonsillitis.
Herba Ceratopteridis Thalictroidis belongs to pteridophyta, is Parkeriaceae (Parkeriaceae), be born in both sides, remote, thickly forested mountains ditch or the water or dark and damp ground, formal name used at school is Ceratopteris thalictroides (Linn.) Brongn, (" Chinese Higher plant illustrated handbook " the 1st, Science Press's version in 1972).Be 1 year aquatic draft, green succulence, mesophyll matter, root stock is short, stands up, and leafage gives birth to.Blade is about the 10-35 centimetre, smooth no hair, two types, two to three times pinniform decomposite leafs, nutrition petiole and blade are isometric approximately, and netted veins do not have free scun between mesh, be the shape of growing thickly, the long and narrow ellipse of trophophyll, blade is shorter, sliver broad, flat, grass green or yellow green, the pinnule depression often has adventitious bud.Sporophyll is then longer, divide thinner, the long and narrow linear drastic crack of leaf, Sporangium then is born in the warp place, edge of sporophyll.It is distributed widely in ground such as China Jiangsu, Zhejiang, Fujian, Taiwan, Guangdong, Yunnan, Sichuan, Hubei and Anhui.Herba Ceratopteridis Thalictroidis also originates in Southeast Asia and Australia; But it is scattered to distribute, as one of China's protective plant.China's example that has the use Herba Ceratopteridis Thalictroidis to cure the disease among the people.The 1669th page of record of Ming Dynasty's Li Shizhen (1518-1593 A.D.) work Compendium of Material Medica volume two: Herba Ceratopteridis Thalictroidis is like Herba pteridii latiusculi, in the unboiled water.Abnormal smells from the patient is sweet, bitter, cold, nontoxic, and detoxifcation, hemostasis are arranged, and controls effects such as mass in the abdomen, bacillary dysentery, fetal toxicosis, traumatic injury." the thousand kinds of medical herbs in south China " record: Herba Ceratopteridis Thalictroidis " detoxifcation, hemostasis, dysentery relieving, cough-relieving ".
The patent No. is a kind of for the CN1176306A Chinese invention patent discloses in addition is Herba Ceratopteridis Thalictroidis wine and the processing method thereof that raw material is made with the Herba Ceratopteridis Thalictroidis, and this drinking utensils has the heat-clearing and toxic substances removing inflammation-diminishing function, and pharyngolaryngitis, gastroenteritis, hepatitis are had beyond thought unique curative effect.But to so far, the report of not seeing the Herba Ceratopteridis Thalictroidis water extract as yet and in the preparation anti-tumor agent, using.
Summary of the invention
Technical problem to be solved by this invention provides a kind of Ceratopteris richardii extract on the one hand, provides the application of above-mentioned Ceratopteris richardii extract in the preparation anti-tumor agent on the other hand, with the propagation of effective inhibition tumor cell and the apoptosis of promotion tumor cell.
The technical problem that the present invention solves can be achieved through the following technical solutions:
Ceratopteris richardii extract, this Herba Ceratopteridis Thalictroidis water extract can be prepared by following method: take by weighing Herba Ceratopteridis Thalictroidis mummification plant, put into to extract after cleaning and irritate, add distilled water then, heating extraction is also collected primary extracting solution; Add distilled water again, twice of heating extraction also collected the second time and extracting solution for the third time respectively; Merge three times extracting solution, filter and leave standstill in room temperature, filtrate is concentrated into the extractum shape through rotary evaporator, promptly gets Ceratopteris richardii extract.
In the above-mentioned preparation process, when extracting for the first time, the amount that adds distilled water is 7 times of volumes of Herba Ceratopteridis Thalictroidis mummification plant, and the proposition time is 60 minutes; When extracting for the second time, the amount that adds distilled water is 2 times of volumes of Herba Ceratopteridis Thalictroidis mummification plant, and extraction time is 30 minutes; When extracting for the third time, the amount that adds distilled water is 1/3 times of volume of Herba Ceratopteridis Thalictroidis mummification plant, and extraction time is 15 minutes.
Described filtrate is in the room temperature standing over night.
The Ceratopteris richardii extract that is obtained, per 1 gram extractum equals 2.857 gram crude drugs.
When Ceratopteris richardii extract of the present invention is used to prepare anti-tumor agent, can effectively suppress the propagation of tumor cell and the apoptosis of promotion tumor cell.
The specific embodiment
Come further to set forth the beneficial effect of anti-tumor agent aspect the apoptosis of propagation that effectively suppresses tumor cell and promotion tumor cell of Ceratopteris richardii extract of the present invention and this Ceratopteris richardii extract preparation by the following examples.
It should be noted that the following examples only as illustrating, content of the present invention is not limited thereto.
The Herba Ceratopteridis Thalictroidis water extract can be prepared by following method: take by weighing Herba Ceratopteridis Thalictroidis mummification plant, with twice of distilled water rinsing, put into to extract and irritate, 7 times of volume distilled waters that add Herba Ceratopteridis Thalictroidis mummification plant then, with slow fire boiling 60 minutes, collect primary extracting solution, add the distilled water of 2 times of volumes again, with slow fire boiling 30 minutes, collect secondary extracting solution, add the distilled water of 1/3 volume at last, decocted 15 minutes, collect extracting solution for the third time, merge three times extracting solution, with 8 layers of filtered through gauze, room temperature standing over night, be concentrated into extractum shape (1 gram extractum=2.857 gram crude drugs) through rotary evaporator, promptly get the Herba Ceratopteridis Thalictroidis water extract.
The HL-60 tumor cell proliferation is suppressed and apoptosis is tested the application of this Ceratopteris richardii extract in preparing anti-tumor agent is described below by Ceratopteris richardii extract.
Test material:
1). Ceratopteris richardii extract: after being made into the concentration of 0.1g/ml with extractum (1 gram=2.857 gram crude drugs), sterilization, 4 ℃ of refrigerators are preserved.
2) .HL-60 cell: the last marine growth of human leukaemia cell is bought, and treats that cell is in exponential phase and promptly can be used for experiment.
3). newborn calf serum (Fcs): magnificent company, 56 ℃, deactivation in 30 minutes.
Test method:
The preparation of the experimental drug of Ceratopteris richardii extract: take by weighing Ceratopteris richardii extract 1 gram with electronic balance, fully dissolve with the deionization distilled water, its concentration is 100mg/ml.15 minutes postcooling of autoclave up sterilization are put into 4 ℃ of refrigerators and are preserved stand-by.
Cell culture: the RPMI RPMI-1640 with containing 10% calf serum places 37 ℃, 5%CO
2Saturated humidity incubator cultured cell.Went down to posterity once with standby in per 48 hours.The trophophase cell of taking the logarithm during drug treating, cell concentration is adjusted into 1*10
5Individual/ml, be made into different systems with the Herba Ceratopteridis Thalictroidis medicine of 10% calf serum, RPMI1640 culture fluid and variable concentrations respectively, be inoculated in 96 orifice plates every hole 0.1ml respectively.Establish 8 holes at every, put saturated humidity, 37 degree Celsius, 5%CO
2The concentration incubator is cultivated, leave standstill cultivate 2～3 days to be detected.
Observed result sees Table 1 and table 2.
Table 1: the Herba Ceratopteridis Thalictroidis water extract is to the influence of HL-60 tumor cell proliferation effect
Component groups n X ± S
The concentration of Herba Ceratopteridis Thalictroidis water extract (mg/ml)
Matched group 37 ± 1
1 3 13.33±2.08
10 3 25.67±1.53
20 3 35.67±2.52★
30 3 44.33±2.08★
Annotate: compare with matched group, check through t: P＜0.01, ★ P＜0.001
Table 2: the Herba Ceratopteridis Thalictroidis water extract is to the influence of HL-60 apoptosis of tumor cells rate
Component groups n X ± S
The concentration of Herba Ceratopteridis Thalictroidis water extract (mg/ml)
Matched group 3 10.67+1.53
1 3 16+2
10 3 30.33+2.52★
20 3 38.33+1.52★
30 3 48.33+4.52★
Annotate: compare with matched group, check through t: P＜0.05, ★ P＜0.01
The result shows that the Herba Ceratopteridis Thalictroidis water extract can increase the mortality rate of HL-60 tumor cell, and behind the Herba Ceratopteridis Thalictroidis medicine of adding variable concentrations, the mortality rate of cell obviously increases, and mortality rate is with concentration linear growth (r=0.996282).The Herba Ceratopteridis Thalictroidis water extract can be induced the apoptosis of HL-60 tumor cell simultaneously, and behind the Herba Ceratopteridis Thalictroidis medicine of adding variable concentrations, the apoptosis rate of cell obviously increases, and apoptosis rate is with concentration linear growth (r=0.992973).
Claims (5)
1, Ceratopteris richardii extract is characterized in that: prepared by following method: take by weighing Herba Ceratopteridis Thalictroidis mummification plant, put into to extract after cleaning and irritate, add distilled water then, heating extraction is also collected primary extracting solution; Add distilled water again, twice of heating extraction also collected the second time and extracting solution for the third time respectively; Merge three times extracting solution, filter and leave standstill in room temperature, filtrate is concentrated into the extractum shape through rotary evaporator, promptly gets Ceratopteris richardii extract.
2, Ceratopteris richardii extract according to claim 1 is characterized in that: when extracting for the first time, the amount that adds distilled water is 7 times of volumes of Herba Ceratopteridis Thalictroidis mummification plant, and the proposition time is 60 minutes; When extracting for the second time, the amount that adds distilled water is 2 times of volumes of Herba Ceratopteridis Thalictroidis mummification plant, and extraction time is 30 minutes; When extracting for the third time, the amount that adds distilled water is 1/3 times of volume of Herba Ceratopteridis Thalictroidis mummification plant, and extraction time is 15 minutes.
3, Ceratopteris richardii extract according to claim 1 is characterized in that: described filtrate is in the room temperature standing over night.
4, Ceratopteris richardii extract according to claim 1 is characterized in that: the Ceratopteris richardii extract that is obtained, per 1 gram extractum equals 2.857 gram crude drugs.
5, the application of a kind of Ceratopteris richardii extract as claimed in claim 1 in the preparation anti-tumor agent.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101849975B (en) * | 2010-06-18 | 2012-05-23 | 福建省立医院 | Application of moonwort extract in preparation of medicament for inhibiting tumor metastasis |
| CN103636499A (en) * | 2013-12-05 | 2014-03-19 | 长春大学 | Rapid induction method for inserting adventitious bud on bean halve green leaf |
| CN106358831A (en) * | 2016-08-31 | 2017-02-01 | 界首市金玖江农业发展有限公司 | Walnut cuttage and seedling culture method |
| CN111991474A (en) * | 2020-09-07 | 2020-11-27 | 深圳市桦健量子生物科技有限公司 | Lucid ganoderma oral liquid and preparation method thereof |
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2005
- 2005-08-29 CN CN 200510029187 patent/CN1733000A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101849975B (en) * | 2010-06-18 | 2012-05-23 | 福建省立医院 | Application of moonwort extract in preparation of medicament for inhibiting tumor metastasis |
| CN103636499A (en) * | 2013-12-05 | 2014-03-19 | 长春大学 | Rapid induction method for inserting adventitious bud on bean halve green leaf |
| CN106358831A (en) * | 2016-08-31 | 2017-02-01 | 界首市金玖江农业发展有限公司 | Walnut cuttage and seedling culture method |
| CN111991474A (en) * | 2020-09-07 | 2020-11-27 | 深圳市桦健量子生物科技有限公司 | Lucid ganoderma oral liquid and preparation method thereof |
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