CN1726981A - Antivirus medical broth of compound Neilupaer - Google Patents
Antivirus medical broth of compound Neilupaer Download PDFInfo
- Publication number
- CN1726981A CN1726981A CN 200510200390 CN200510200390A CN1726981A CN 1726981 A CN1726981 A CN 1726981A CN 200510200390 CN200510200390 CN 200510200390 CN 200510200390 A CN200510200390 A CN 200510200390A CN 1726981 A CN1726981 A CN 1726981A
- Authority
- CN
- China
- Prior art keywords
- parts
- neilupaer
- antivirus
- compound
- detect
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
An antiviral decoction for improving immunity and preventing SARS is prepared from nipagin ethylester, benzoic acid, proteose, and 7 Chinese-medicinal materials including water lily flower, chicory herb, dwarf flowering cherry seed, etc.
Description
Technical field
The present invention relates to composite medicine and production method thereof, is a kind of antivirus medical broth of compound Neilupaer and preparation technology thereof, is specially adapted to the prevention to atypical pneumonia.
Background technology
Atypical pneumonia is that a kind of coronavirus by name causes that infectiousness is very strong, serious threat human physical and mental health's respiratory system disease.World Health Organization (WHO) is referred to as SARS (Severe Acute Respiratory Syndrome) (SevereAcuute Respiratory Syndromes) with this severe acute respiratory syndrome, is called for short SARS.
China's Uygur medicine pharmacy is the important component part of Traditional Chinese Medicine, and the Chinese people of Uygur have formed the complete uighur medicine theoretical system of a cover in long-term practical experience of life.Atypical pneumonia, Uygur's medical science is classified one of epidemic diseases as, belong to the category of " Wa Ba " disease (being pestilence), and have realized that this class disease has stronger infectiousness, promptly propagate very fast, the higher epidemiology characteristics of falling ill, as in medical scholar's monumental work that A Weisenna shows great before more than 1,000 years " Airy Ka Nongfei carry than " (curing allusion quotation greatly) with more than 800 year before famous adding in the Malian fourth Acker monograph that Sa Layi shows " Acker Sa Layi " (white palace) with field uighur medicine scholar, once put down in writing this type of disease, and it is referred to as: " exerting wooden Ni Yawabayi ", " assorted carry day also Wa Bayi " (pestilence pneumonia), " platform micromicrowatt crust is complied with " (pestilence heat pyrexia), " Areia of Soviet Union is than Si Wabayi " (pestilence dry cough) etc.Uygur's medical science is thought and is fled in air and putrid special " adding Lars wood " (i.e. virus), by approach such as respiratory tracts, invade human body, make lungs makings that xeothermic property or the impatient drastic changeization of bile matter take place, thereby cause the generation of " Sai Feilayi wood Tai Rekai " (promptly burning bile matter), impel the outburst of this disease.And think that this disease mostly occurs in the relatively poor crowd of lungs vigour weakness, quality, by aerial infection; More symptom is seen and is the heating fever, and the headache feeling of fullness in the head feels cold that it is cold to be afraid of, systemic pain, dyspnea, violent cough mostly is dry cough, pertinacious phlegm is not changed, the time apoplexy due to phlegm blood streak etc. arranged.If in time do not prevent, therapeutic effect is relatively poor.
Summary of the invention
The invention provides a kind of antivirus medical broth of compound Neilupaer and preparation technology thereof, its antivirus medical broth of compound Neilupaer can strengthen humoral immunization and non-specific immunity, help antibody to generate, build up resistance, thereby be specially adapted to prevention atypical pneumonia.
One of technical scheme of the present invention is achieved like this: a kind of antivirus medical broth of compound Neilupaer, it contains 16 parts to 24 parts of 100 parts to 180 parts of Flos nymphaeae (Nymphaea teragona Georgi)s, 100 parts to 180 parts of Herba Cichoriis, 100 parts to 180 parts of Prunus humilis, 80 parts to 120 parts of Flos althaeae roseaes, 80 parts to 120 parts of Hyssopus officinalis, 80 parts to 120 parts of Herba Achilleaes, 48 parts to 72 parts of saxual Fructus Vitis viniferaes, 2% ethyl hydroxybenzoate of conventional survival dose, 10% benzoic acid of conventional survival dose, protein sugar by parts by weight of raw materials.
Two of technical scheme of the present invention is achieved like this: the preparation technology of above-mentioned antivirus medical broth of compound Neilupaer is undertaken by following step: the Flos nymphaeae (Nymphaea teragona Georgi) of above-mentioned requirement, Herba Cichorii, Prunus humilis, Flos althaeae roseae, Hyssopus officinalis, in seven kinds of raw materials of Herba Achilleae and saxual Fructus Vitis viniferae, gross weight by seven kinds of raw materials adds 6 times to 10 times water gagings decoctions 1 time to 3 times, time is 0.5 hour to 2.5 hours, merge extractive liquid,, filter, filtrate decompression is concentrated into medical material: medicinal liquid is 1: 3 to 1: 7 o'clock, in per 100 milliliters of medicinal liquids, add concentration and be 1 milliliter to 3 milliliters of 1% ZTC1+1-III type natural clarifying agent B component, add concentration again and be 2 milliliters to 6 milliliters of 1% ZTC1+1-III type natural clarifying agent A components, stir evenly, 60 ℃ to 70 ℃ water bath heat preservations 0.5 hour to 1.5 hours, 2 ℃ to 6 ℃ left standstill 20 hours to 25 hours, filter, filtrate decompression is concentrated into that relative density is 1.05 to 1.08 in the time of 60 ℃, the 2% ethyl hydroxybenzoate ethanol liquid that adds conventional survival dose, the 10% benzoic acid liquid of conventional survival dose and the protein sugar of requirement add water and make its liquid gross weight to 1000 part obtain antivirus medical broth of compound Neilupaer.
The present invention is according to uighur medicine scientific principle opinion, through a large amount of experiments with preferably just obtain.The compound recipe that antivirus medical broth of compound Neilupaer of the present invention is made up of seven flavor medicines such as Flos nymphaeae (Nymphaea teragona Georgi)s, its Herba indigoferae Pseudotinctoriae " Flos nymphaeae (Nymphaea teragona Georgi) " dimension language claims " Neilupaer ", the title of this preparation is the dimension language title according to Flos nymphaeae (Nymphaea teragona Georgi), adds dosage form name formation.
Antivirus medical broth of compound Neilupaer of the present invention, constant product quality, be safe under the clinical dosage condition, its amount can obviously increase the generation of mice serum hemolysin, and external bone-marrow-derived lymphocyte is cultivated tangible proliferation function, the T lymphocyte there is not obvious influence, can obviously strengthen the phagocytic function of Turnover of Mouse Peritoneal Macrophages, it can strengthen humoral immunization and non-specific immunity, helps antibody to generate, build up resistance, thereby be specially adapted to prevention atypical pneumonia.Antivirus medical broth of compound Neilupaer preparation technology of the present invention is convenient to realize industrialization.
The specific embodiment
The present invention is not subjected to the restriction of following embodiment, can determine concrete according to the technical scheme and the practical situation of the invention described above
Embodiment.
Embodiment 1, and this antivirus medical broth of compound Neilupaer contains 16 parts of 100 parts of Flos nymphaeae (Nymphaea teragona Georgi)s, 100 parts of Herba Cichoriis, 100 parts of Prunus humilis, 80 parts of Flos althaeae roseaes, 80 parts of Hyssopus officinalis, 80 parts of Herba Achilleaes, 48 parts of saxual Fructus Vitis viniferaes, 2% ethyl hydroxybenzoate of conventional survival dose, 10% benzoic acid of conventional survival dose, protein sugar by parts by weight of raw materials.
Embodiment 2, and this antivirus medical broth of compound Neilupaer contains 24 parts of 180 parts of Flos nymphaeae (Nymphaea teragona Georgi)s, 180 parts of Herba Cichoriis, 180 parts of Prunus humilis, 120 parts of Flos althaeae roseaes, 120 parts of Hyssopus officinalis, 120 parts of Herba Achilleaes, 72 parts of saxual Fructus Vitis viniferaes, 2% ethyl hydroxybenzoate of conventional survival dose, 10% benzoic acid of conventional survival dose, protein sugar by parts by weight of raw materials.
Embodiment 3, and this antivirus medical broth of compound Neilupaer contains 24 parts of 100 parts of Flos nymphaeae (Nymphaea teragona Georgi)s, 180 parts of Herba Cichoriis, 100 parts of Prunus humilis, 120 parts of Flos althaeae roseaes, 80 parts of Hyssopus officinalis, 120 parts of Herba Achilleaes, 48 parts of saxual Fructus Vitis viniferaes, 2% ethyl hydroxybenzoate of conventional survival dose, 10% benzoic acid of conventional survival dose, protein sugar by parts by weight of raw materials.
Embodiment 4, and this antivirus medical broth of compound Neilupaer contains 16 parts of 180 parts of Flos nymphaeae (Nymphaea teragona Georgi)s, 100 parts of Herba Cichoriis, 180 parts of Prunus humilis, 80 parts of Flos althaeae roseaes, 120 parts of Hyssopus officinalis, 80 parts of Herba Achilleaes, 72 parts of saxual Fructus Vitis viniferaes, 2% ethyl hydroxybenzoate of conventional survival dose, 10% benzoic acid of conventional survival dose, protein sugar by parts by weight of raw materials.
Embodiment 5, and this antivirus medical broth of compound Neilupaer contains 24 parts of 100 parts of Flos nymphaeae (Nymphaea teragona Georgi)s, 100 parts of Herba Cichoriis, 180 parts of Prunus humilis, 120 parts of Flos althaeae roseaes, 80 parts of Hyssopus officinalis, 80 parts of Herba Achilleaes, 72 parts of saxual Fructus Vitis viniferaes, 2% ethyl hydroxybenzoate of conventional survival dose, 10% benzoic acid of conventional survival dose, protein sugar by parts by weight of raw materials.
Embodiment 6, and this antivirus medical broth of compound Neilupaer contains 16 parts of 180 parts of Flos nymphaeae (Nymphaea teragona Georgi)s, 180 parts of Herba Cichoriis, 100 parts of Prunus humilis, 80 parts of Flos althaeae roseaes, 120 parts of Hyssopus officinalis, 120 parts of Herba Achilleaes, 48 parts of saxual Fructus Vitis viniferaes, 2% ethyl hydroxybenzoate of conventional survival dose, 10% benzoic acid of conventional survival dose, protein sugar by parts by weight of raw materials.
Embodiment 7, and this antivirus medical broth of compound Neilupaer contains 24 parts of 100 parts of Flos nymphaeae (Nymphaea teragona Georgi)s, 100 parts of Herba Cichoriis, 100 parts of Prunus humilis, 80 parts of Flos althaeae roseaes, 120 parts of Hyssopus officinalis, 120 parts of Herba Achilleaes, 72 parts of saxual Fructus Vitis viniferaes, 2% ethyl hydroxybenzoate of conventional survival dose, 10% benzoic acid of conventional survival dose, protein sugar by parts by weight of raw materials.
Embodiment 8, and this antivirus medical broth of compound Neilupaer contains 16 parts of 180 parts of Flos nymphaeae (Nymphaea teragona Georgi)s, 180 parts of Herba Cichoriis, 180 parts of Prunus humilis, 120 parts of Flos althaeae roseaes, 80 parts of Hyssopus officinalis, 80 parts of Herba Achilleaes, 48 parts of saxual Fructus Vitis viniferaes, 2% ethyl hydroxybenzoate of conventional survival dose, 10% benzoic acid of conventional survival dose, protein sugar by parts by weight of raw materials.
Embodiment 9, and this antivirus medical broth of compound Neilupaer contains 20 parts of 140 parts of parts of Flos nymphaeae (Nymphaea teragona Georgi), 140 parts of Herba Cichoriis, 140 parts of Prunus humilis, 100 parts of Flos althaeae roseaes, 100 parts of Hyssopus officinalis, 100 parts of Herba Achilleaes, 60 parts of saxual Fructus Vitis viniferaes, 2% ethyl hydroxybenzoate of conventional survival dose, 10% benzoic acid of conventional survival dose, protein sugar by parts by weight of raw materials.
Antivirus medical broth of compound Neilupaer in the foregoing description can obtain by following preparation technology:
Flos nymphaeae (Nymphaea teragona Georgi) at above-mentioned requirement, Herba Cichorii, Prunus humilis, Flos althaeae roseae, Hyssopus officinalis, in seven kinds of raw materials of Herba Achilleae and saxual Fructus Vitis viniferae, adding 6 times or 10 times of water gagings by the gross weight of seven kinds of raw materials decocts 1 time or 3 times, time is 0.5 hour or 2.5 hours, merge extractive liquid,, filter, filtrate decompression is concentrated into medical material: medicinal liquid is 1: 3 or 1: 7 o'clock, in per 100 milliliters of medicinal liquids, add concentration and be 1 milliliter or 3 milliliters of 1% ZTC1+1-III type natural clarifying agent B component, add concentration again and be 2 milliliters or 6 milliliters of 1% ZTC1+1-III type natural clarifying agent A components, stir evenly, 60 ℃ or 70 ℃ of water bath heat preservations 0.5 hour or 1.5 hours, 2 ℃ or 6 ℃ left standstill 20 hours or 25 hours, filter, filtrate decompression is concentrated into that relative density is 1.05 or 1.08 in the time of 60 ℃, adds 2% ethyl hydroxybenzoate ethanol liquid of conventional survival dose, the 10% benzoic acid liquid of conventional survival dose and the protein sugar of requirement add water and make its liquid gross weight to 1000 part obtain antivirus medical broth of compound Neilupaer.
In the present invention: used ethyl hydroxybenzoate ethanol liquid and benzoic acid liquid all are pharmaceutics antiseptic commonly used, its consumption all is controlled in the scope of conventional survival dose, for example: in the 4th edition " pharmaceutics " that Bi Dianzhou chief editor, People's Health Publisher publish, antiseptic kind, consumption that pharmaceuticss such as ethyl hydroxybenzoate ethanol liquid and benzoic acid liquid are commonly used have all been done detailed argumentation; Used ZTC1+1-III type natural clarifying agent A and ZTC1+1-III type natural clarifying agent B are the food additive that adopts among the DB12-065-96 of tianjin local standards.
It is as follows below the foregoing description products obtained therefrom to be that antivirus medical broth of compound Neilupaer carries out pharmacodynamics test, toxicological experiment and stability experiment:
One. to the influence of mice serum hemolysin generation
Experiment material: 40 of NIH mices; Tried thing: the foregoing description products obtained therefrom antivirus medical broth of compound Neilupaer.
Experimental technique:
The preparation of 1 sheep red blood cell (SRBC) suspension: under aseptic condition, get blood 50ml, place the sterilization conical flask that fills glass bead, shake 10 minutes, add the Alsever liquid that is equivalent to 2 times of Sanguis caprae seu ovis volumes and shake up with except that defibrinating from healthy adult sheep external jugular vein.Put in 4 ℃ of refrigerators and to preserve standbyly, face the time spent and wash (centrifugal 5 minutes of 1500rpm) 3 times with normal saline, abandon supernatant, with normal saline (V/V) dilution in 3: 5, making blood cell concentration is 2,000,000,000/mL.
2. immunity and administration: 40 of mices are divided at random according to body weight, sex: the normal saline matched group; Antivirus medical broth of compound Neilupaer of the present invention is little, in, big (3.0g/Kg, 6.0g/Kg, 12.0g/Kg) 3 various dose groups.Continuous 8 days of ig administration, 2h after the administration in the 3rd day, each only organizes equal lumbar injection 20% sheep erythrocyte suspension 0.2ml/, and back 5 days eye sockets of immunity are got blood, survey serum hemolysin.
3, hemolysin is measured: eye socket is got blood in the 1ml plastic centrifuge tube, places 1h, and centrifugal 10 minutes of 2000rpm gets serum, experimentizes with according to the form below after 500 times of the normal saline dilutions.Be incubated 10 minutes then in 37 ℃ of water-baths, 2000rpm is centrifugal 5 minutes after the ice bath cessation reaction, gets supernatant 1ml, and other adds Dou Shi reagent 3ml in vitro.Get sheep red blood cell (SRBC) diluent 0.25ml, Dou Shi reagent 4ml simultaneously in another in vitro, fully shake up, place after 10 minutes, each Guan Jun does blank with contrast in 540nm, measure respectively and respectively manage trap.
The sample cell control tube
500 times of dilute serum 1ml
Normal saline? 1ml
Sheep red blood cell (SRBC) diluent 0.5ml 0.5ml
1: 10 guinea pig serum diluent 1ml 1ml
4, calculate: the half hemolysis value HC that calculates every mice sample by following formula
50
Sample HC
50=(inhale during absorption of sample degree average/sheep red blood cell HD50
Receipts degree value) * serum diluting multiple
The HC of each group of administration
50And mean t check between matched group work group.The results are shown in Table 1
Table 1 antivirus medical broth of compound Neilupaer of the present invention is to the influence of serum hemolysin (X ± SD)
Group n dosage (g/kg) HC
50
Normal saline group 10 10.0 53.2 ± 20.3
Gained decoction 10 3.0 64.8 ± 36.3* of the present invention
10 6.0 80.3±35.7**
10 12.0 95.5±39.2**
* p＞0.05, * * p＜0.05, * * * p＜0.01 all with the NS group relatively.
Conclusion: in the presentation of results antivirus medical broth of compound Neilupaer of the present invention, heavy dose can obviously increase the generation of mice serum hemolysin, and nonspecific immunity is had potentiation, can increase the generation of antibody, strengthen with dosage increase effect.
Two. the lymphopoietic influence of mice B, T
Material: NIH mice, MTT, MERCK, Lot474314105.Microplate reader, Bio-Rad, Mode 550.ConA, Beijing Medical Depot packing, LPS, Sigma, lot number, 69H4046.
Method: get 6 of NIH mices, body weight 18-22 gram, male, to pluck the eyeball blood-letting and cause death, the sterilization of 0.1% bromo geramine is cut open the belly and is got spleen, shreds 100 mesh sieves, and the centrifugal supernatant that goes adds 0.17mol/L NH
4Cl removes erythrocyte, washes splenocyte 3 times with HBSS liquid, adds the 10%NBS-RPMI-1640 culture fluid, and adjusting cell concentration is 1 * 10
6Individual/ml.Every hole adds splenocyte suspension 100ul/ hole, medicinal liquid 100ul, ConA (5mg/L) 50ul, or LPS (10mg/L) 50ul (above operation is all carried out under aseptic condition), 37 ℃, 5%CO
2Temperature was incubated 44 hours, added MTT (1g/L) 50ul, and 37 ℃ of incubations 4 hours remove supernatant, added DMS0300ul and shook up, and microplate reader 570nm measures.(the results are shown in Table 2)
Table 2 antivirus medical broth of compound Neilupaer of the present invention
To the lymphopoietic influence of mice B, T (n=3 X ± SD)
Mg/L (crude drug) ConA resting stage (5mg/L) LPS (10mg/L)
Contrast 0.0 0.453 ± 0.121 0.371 ± 0.142 0.433 ± 0.142
0.0001 0.442±0.132 0.363±0.122 0.437±0.133
0.001 0.463±0.114 0.350±0.123 0.545±0.153
**
0.01 0.445±0.123 0.354±0.131 0.576±0.142
**
0.1 0.467±0.111 0.360±0.124 0.577±0.139
**
1.0 0.434±0.121 0.353±0.126 0.669±0.133
**
10.0 0.440±0.132 0.340±0.126
*?0.565±0.139
**
100.0 0.442±0.134 0.345±0.119
*?0.443±0.140
All compare with matched group * p＜0.05, * * * p＜0.01.
Experimental result shows that antivirus medical broth of compound Neilupaer of the present invention has proliferation function to external bone-marrow-derived lymphocyte between 0.001-10mg/L, can generate by enhancing antibody, and dose-effect relationship is arranged, to the not influence of T lymphopoiesis.
Three. to the influence of Turnover of Mouse Peritoneal Macrophages phagocytic function
Method: 6 of (1) NIH mices, ip 0.5% starch normal saline (boiling 15 minutes) 0.5ml/ only plucks the eyeball blood-letting and causes death lumbar injection HBSS 2ml after 3 days, soft abdominal part, and in abdominal scissors one osculum, with dropper sucking-off peritoneal fluid, centrifugal 1500rpm 10 minutes, abandon supernatant, add HBSS liquid 5ml, it is inferior to give a baby a bath on the third day after its birth, and transfers cell concentration to 1 * 10 with 15%NBS-1640 nutritional solution (not containing HEPES)
6Individual/ml, more than operation is all carried out under 0-4 ℃ of aseptic condition.
(2) white Portugal's bacterium liquid preparation
Wash white Portugal bacterium on the solid medium with physiological saline solution, transfer A=0.5 (32 times of the reuse 15%NBS-1640 nutritional solution dilutions of λ=620nm) with normal saline.
(3) antivirus medical broth of compound Neilupaer: be made into 0.001 grams per milliliter concentration with normal saline, the time spent causes desired concn with the nutritional solution dilution.
(4) concrete operations:
The white Portugal of group dosage M Φ bacterium nutritive medical liquid
(μg·L
-1) (μl) (μl) (μl) (μl)
| NS | ---- | --- | ?100 | ?200 | |
| NS+MΦ | --- | 100 | ?100 | ?100 | |
| Gained decoction of the present invention | 1.0 | 100 | 100 | ?100 | |
| 10.0 | 100 | 100 | ?100 | ||
| 100.0 | 100 | 100 | ?100 |
Table 3 antivirus medical broth of compound Neilupaer of the present invention
Influence to the peritoneal macrophage phagocytic function
Group dosage exit dose phagocytic index (PI)
μg·L
-1 dmp ％
NS group-5680.2 ± 1121.0
NS+M Φ group-3625.3 ± 1,324 37.2
Gained decoction 1.0 2624.0 of the present invention ± 1031.0 54.3
10.0 2046.0±894.0 63.8
100.0 1623.0±886.0 72.1
The result shows: each dosage group of antivirus medical broth of compound Neilupaer of the present invention all has the effect that strengthens the peritoneal macrophage phagocytic function, and 10.0,100.0 μ g/ml dosage group differences have significance, and effect strengthens with dosage.
Conclusion: antivirus medical broth of compound Neilupaer of the present invention can obviously increase the generation of mice serum hemolysin at 6.0g/Kg, 12.0g/Kg dosage, between 0.001-10.0mg/L, external bone-marrow-derived lymphocyte cultivation there is tangible proliferation function, the T lymphocyte is not had obvious influence, and external 10-100 μ g/L can obviously strengthen the phagocytic function of Turnover of Mouse Peritoneal Macrophages.Comprehensive above result of the test explanation can strengthen humoral immunization and non-specific immunity, helps antibody to generate, and builds up resistance.
In sum, generate by measuring serum hemolysin, mtt assay is surveyed the propagation of T, bone-marrow-derived lymphocyte,
3The H-TdR method is measured the phagocytic function of Turnover of Mouse Peritoneal Macrophages.Result: antivirus medical broth of compound Neilupaer (4.0 of the present invention, .0g/kg) can obviously strengthen the mice serum hemolysin and generate (P＜0.05), antivirus medical broth of compound Neilupaer of the present invention (0.01-10mg/L) can promote the external bone-marrow-derived lymphocyte propagation of mice, the T lymphocyte is not had obvious influence, can strengthen the external phagocytic function of Turnover of Mouse Peritoneal Macrophages.Conclusion: antivirus medical broth of compound Neilupaer of the present invention can the enhancing non-specific immunity function.
Three, acute toxicity test purpose:
1. purpose: observe antivirus medical broth of compound Neilupaer and give mice acute toxic reaction and death condition that oral back produces.
2. animal: the NIH mice, male and female half and half, body weight 19.9 ± 1.4g, does Xinjiang medical experiment animal center provide, and produces certificate: the moving word the (96) 16 of new doctor? 01.
3. medicine: the foregoing description gained antivirus medical broth of compound Neilupaer.
4. method and result:
(1) trial test: choose 4 of mices, antivirus medical broth of compound Neilupaer is irritated stomach with the 0.4ml/10g capacity, observes 24 hours, does not see a death, and spirit and active state are also no abnormal, and explanation can't be measured LD
50, can only do the maximum tolerated dose experiment in accordance with the law.
(2) mensuration of maximum tolerated dose (MTD)
Choose 20 of healthy mices, male and female half and half, body weight 19.9 ± 1.4g, with 0.4ml/10g gastric infusion secondary, 6 hours at interval, observed four hours continuously after the administration, every morning, an observation in afternoon afterwards once, observed seven days the result continuously: it is no abnormal with active state to be tried mice spirit, and also none is only dead.The mice average weight was 24.1 ± 1.3g when experiment finished.So maximum tolerated dose should be 22.4g/kg by crude drug.
5. conclusion
The maximum tolerated dose of antivirus medical broth of compound Neilupaer is counted 22.4g/kg by crude drug, is equivalent to 80 times of adult's (body weight is by 60kg) per kilogram every day consumption.Illustrate that this preparation does not have overt toxicity, oral medication is safe.
Four, stability experiment
Carry out the stability test of embodiment of the invention gained antivirus medical broth of compound Neilupaer with reference to the method for regulation under two appendix XI of Chinese Pharmacopoeia version in 2000 C medicine stability test guideline item.
1. accelerated test
Get 1,2,3 three batches of antivirus medical broth of compound Neilupaer test samples of the foregoing description, press commercially available back, place in artificial intelligence's climate box, adjust the temperature to 40 ± 2 ℃, relative humidity 75 ± 5% was placed sampling interval time: 06 months, 1,2,3,6 months, finished at present all the elements of accelerated test, result of the test sees Table 1.
Preparation stability result of the test under table 1 acceleration environment
Sample: embodiment 1 gained antivirus medical broth of compound Neilupaer
| 0 month: 2003..8.6 | January: 2003..9.6: | February: 2003.10.6 | March: 2003.11.6 | June: 2004.2.8 | ||
| Character | Yellowish-brown is to brown liquid; Gas perfume (or spice), it is sweet to distinguish the flavor of, little hardship | With 0 month | With 0 month | With 0 month | With 0 month | |
| Differentiate | Another name for Sichuan Province TLC in last of the ten Heavenly stems Herba Achilleae TLC | Detect Flos althaeae roseae and detect Herba Achilleae | Detect Flos althaeae roseae and detect Herba Achilleae | Detect Flos althaeae roseae and detect Herba Achilleae | Detect Flos althaeae roseae and detect Herba Achilleae | Detect Flos althaeae roseae and detect Herba Achilleae |
| Check | (1) loading amount (2) relative density (3) pH value | Up to specification 1.023 6.06 | Up to specification 1.024 6.03 | Up to specification 1.027 6.01 | Up to specification 1.020 6.08 | Up to specification 1.019 6.03 |
| Content | Chlorogenic acid mg/ props up | 15.22 | 15.73 | 15.69 | 15.42 | 15.79 |
| Health examination | Total number of bacteria (/ml) total number of molds (/ml) escherichia coli | Do not detect below 50 below 100 | Do not detect below 50 below 100 | Do not detect below 50 below 100 | Do not detect below 50 below 100 | Do not detect below 50 below 100 |
Sample: embodiment 2 gained antivirus medical broth of compound Neilupaer
| 0 month: 2003..8.6 | January: 2003..9.6: | February: 2003.10.6 | March: 2003.11.6 | June: 2004.2.8 | ||
| Character | Yellowish-brown is to brown liquid; Gas perfume (or spice), it is sweet to distinguish the flavor of, little hardship | With 0 month | With 0 month | With 0 month | With 0 month | |
| Differentiate | Flos althaeae roseae TLC Herba Achilleae TLC | Detect Flos althaeae roseae and detect Herba Achilleae | Detect Flos althaeae roseae and detect Herba Achilleae | Detect Flos althaeae roseae and detect Herba Achilleae | Detect Flos althaeae roseae and detect Herba Achilleae | Detect Flos althaeae roseae and detect Herba Achilleae |
| Check | (1) loading amount (2) relative density (3) pH value | Up to specification 1.023 6.44 | Up to specification 1.029 6.19 | Up to specification 1.023 6.43 | Up to specification 1.022 6.28 | Up to specification 1.022 6.38 |
| Content | Chlorogenic acid mg/ props up | 15.42 | 15.42 | 15.58 | 15.59 | 15.28 |
| Health examination | Total number of bacteria (/ml) total number of molds (/ml) escherichia coli | Do not detect below 50 below 100 | Do not detect below 50 below 100 | Do not detect below 50 below 100 | Do not detect below 50 below 100 | Do not detect below 50 below 100 |
Sample: embodiment 3 gained antivirus medical broth of compound Neilupaer
| 0 month: 2003.8.6 | January: 2003..9.6: | February: 2003.10.6 | March: 2003.11.6 | June: 2004.2.8 | ||
| Character | Yellowish-brown is to brown liquid; Gas perfume (or spice), it is sweet to distinguish the flavor of, little hardship | With 0 month | With 0 month | With 0 month | With 0 month | |
| Differentiate | Flos althaeae roseae TLC Herba Achilleae TLC | Detect Flos althaeae roseae and detect Herba Achilleae | Detect Flos althaeae roseae and detect Herba Achilleae | Detect Flos althaeae roseae and detect Herba Achilleae | Detect Flos althaeae roseae and detect Herba Achilleae | Detect Flos althaeae roseae and detect Herba Achilleae |
| Check | (1) loading amount (2) relative density (3) pH value | Up to specification 1.025 6.24 | Up to specification 1.024 6.33 | Up to specification 1.027 6.41 | Up to specification 1.020 6.38 | Up to specification 1.019 6.33 |
| Content | Chlorogenic acid mg/ props up | 16.41 | 16.37 | 16.29 | 16.21 | 16.22 |
| Health examination | Total number of bacteria (/ml) total number of molds (/ml) escherichia coli | Do not detect below 50 below 100 | Do not detect below 50 below 100 | Do not detect below 50 below 100 | Do not detect below 50 below 100 | Do not detect below 50 below 100 |
2. long-term stable experiment
Get three batches of antivirus medical broth of compound Neilupaer test samples, lot number: 030802,030803 and 030804, press commercially available back, 25 ± 2 ℃ of temperature, stored 24 months sampling interval time: 0 under the condition of relative humidity 60 ± 10%, 3,6,9,12,18,24 months, only obtain preceding 6 months test data at present, the results are shown in Table 2, test still underway.
Table 2 long-term stable experiment preparation preliminarily stabilised test report
Sample: embodiment 5 gained antivirus medical broth of compound Neilupaer
| 0 month: 2003..8.6 | March: 2003.11.6 | June: 2004.2.8 | ||
| Character | Yellowish-brown is to brown liquid; Gas perfume (or spice), it is sweet to distinguish the flavor of, little hardship | With 0 month | With 0 month | |
| Differentiate | Flos althaeae roseae TLC Herba Achilleae TLC | Detect Flos althaeae roseae and detect Herba Achilleae | Detect Flos althaeae roseae and detect Herba Achilleae | Detect Flos althaeae roseae and detect Herba Achilleae |
| Check | (1) loading amount (2) relative density (3) pH value | Up to specification 1.023 6.06 | Up to specification 1.020 6.10 | Up to specification 1.019 6.00 |
| Content | Chlorogenic acid mg/ props up | 15.22 | 15.12 | 15.09 |
| Health examination | Total number of bacteria (/ml) total number of molds (/ml) escherichia coli | Do not detect below 50 below 100 | Do not detect below 50 below 100 | Do not detect below 50 below 100 |
The disease-resistant mould decoction of sample: embodiment 7 gained compound recipe Neilupaers
| 0 month: 2003.8.6 | March: 2003.11.6 | June: 2004.2.8 | ||
| Character | Yellowish-brown is to brown liquid; Gas perfume (or spice), it is sweet to distinguish the flavor of, little hardship | With 0 month | With 0 month | |
| Differentiate | Flos althaeae roseae TLC Herba Achilleae TLC | Detect Flos althaeae roseae and detect Herba Achilleae | Detect Flos althaeae roseae and detect Herba Achilleae | Detect Flos althaeae roseae and detect Herba Achilleae |
| Check | (1) loading amount (2) relative density (3) pH value | Up to specification 1.023 6.44 | Up to specification 1.025 6.25 | Up to specification 1.020 6.30 |
| Content | Chlorogenic acid mg/ props up | ?15.42 | ?15.49 | ?15.20 |
| Health examination | Total number of bacteria (/ml) total number of molds (/ml) escherichia coli | Do not detect below 50 below 100 | Do not detect below 50 below 100 | Do not detect below 50 below 100 |
The disease-resistant mould decoction of sample: embodiment 9 gained compound recipe Neilupaers
| 0 month: 2003..8.6 | March: 2003.11.6 | June: 2004.2.8 | ||
| Character | Yellowish-brown is to brown liquid; Gas perfume (or spice), it is sweet to distinguish the flavor of, little hardship | With 0 month | With 0 month | |
| Differentiate | Flos althaeae roseae TLC Herba Achilleae TLC | Detect Flos althaeae roseae ì and go out Herba Achilleae | Detect Flos althaeae roseae and detect Herba Achilleae | Detect Flos althaeae roseae and detect Herba Achilleae |
| Check | (1) loading amount (2) relative density (3) pH value | Up to specification 1.025 6.24 | Up to specification 1.028 6.22 | Up to specification 1.055 6.18 |
| Content | Chlorogenic acid mg/ props up | 16.41 | 16.21 | 16.22 |
| Health examination | Total number of bacteria (/ml) total number of molds (/ml) escherichia coli | Do not detect below 50 below 100 | Do not detect below 50 below 100 | Do not detect below 50 below 100 |
3. conclusion: 6 months accelerated test result shows that antivirus medical broth of compound Neilupaer adopts commercially available back, 40 ± 2 ℃ of temperature, places 6 months under relative humidity 75 ± 5% conditions, and every index is not seen significant change.
Claims (2)
1, a kind of antivirus medical broth of compound Neilupaer is characterized in that containing 16 parts to 24 parts of 100 parts to 180 parts of Flos nymphaeae (Nymphaea teragona Georgi)s, 100 parts to 180 parts of Herba Cichoriis, 100 parts to 180 parts of Prunus humilis, 80 parts to 120 parts of Flos althaeae roseaes, 80 parts to 120 parts of Hyssopus officinalis, 80 parts to 120 parts of Herba Achilleaes, 48 parts to 72 parts of saxual Fructus Vitis viniferaes, 2% ethyl hydroxybenzoate of conventional survival dose, 10% benzoic acid of conventional survival dose, protein sugar by parts by weight of raw materials.
2, a kind of antivirus medical broth of compound Neilupaer preparation technology according to claim 1, it is characterized in that being undertaken: the Flos nymphaeae (Nymphaea teragona Georgi) of above-mentioned requirement by following step, Herba Cichorii, Prunus humilis, Flos althaeae roseae, Hyssopus officinalis, in seven kinds of raw materials of Herba Achilleae and saxual Fructus Vitis viniferae, gross weight by seven kinds of raw materials adds 6 times to 10 times water gagings decoctions 1 time to 3 times, time is 0.5 hour to 2.5 hours, merge extractive liquid,, filter, filtrate decompression is concentrated into medical material: medicinal liquid is 1: 3 to 1: 7 o'clock, in per 100 milliliters of medicinal liquids, add concentration and be 1 milliliter to 3 milliliters of 1% ZTC1+1-III type natural clarifying agent B component, add concentration again and be 2 milliliters to 6 milliliters of 1% ZTC1+1-III type natural clarifying agent A components, stir evenly, 60 ℃ to 70 ℃ water bath heat preservations 0.5 hour to 1.5 hours, 2 ℃ to 6 ℃ left standstill 20 hours to 25 hours, filter, filtrate decompression is concentrated into that relative density is 1.05 to 1.08 in the time of 60 ℃, the 2% ethyl hydroxybenzoate ethanol liquid that adds conventional survival dose, the 10% benzoic acid liquid of conventional survival dose and the protein sugar of requirement add water and make its liquid gross weight to 1000 part obtain antivirus medical broth of compound Neilupaer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005102003908A CN1329059C (en) | 2005-07-12 | 2005-07-12 | Antivirus medical broth of compound Neilupaer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005102003908A CN1329059C (en) | 2005-07-12 | 2005-07-12 | Antivirus medical broth of compound Neilupaer |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1726981A true CN1726981A (en) | 2006-02-01 |
| CN1329059C CN1329059C (en) | 2007-08-01 |
Family
ID=35926619
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2005102003908A Active CN1329059C (en) | 2005-07-12 | 2005-07-12 | Antivirus medical broth of compound Neilupaer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1329059C (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101264181B (en) * | 2008-04-28 | 2010-06-23 | 汪甬伟 | Auxiliary treating hepatitis Chinese and Western medicines composition containing biphenyldicarboxylate and Chinese medicine extraction |
| CN106039277A (en) * | 2016-08-05 | 2016-10-26 | 王豪鹏 | Compound Neelubar cold-treating granules and production method thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1291729C (en) * | 2003-09-23 | 2006-12-27 | 新疆维吾尔自治区药物研究所 | Antiviral Chinese medicine prepn of Hypericum perforatumand its prepn process |
-
2005
- 2005-07-12 CN CNB2005102003908A patent/CN1329059C/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101264181B (en) * | 2008-04-28 | 2010-06-23 | 汪甬伟 | Auxiliary treating hepatitis Chinese and Western medicines composition containing biphenyldicarboxylate and Chinese medicine extraction |
| CN106039277A (en) * | 2016-08-05 | 2016-10-26 | 王豪鹏 | Compound Neelubar cold-treating granules and production method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1329059C (en) | 2007-08-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1247121C (en) | Longan aril and wolfberry fruit polysaccharide health-care liquid and its preparing method and use | |
| RU2559784C2 (en) | Composition for mitigating fatigue, formulation and using them | |
| CN1921771A (en) | Composition for preventing a cold | |
| WO2020118576A1 (en) | Use of lactobacillus plantarum twk10 in preparation of composition for resistance to post-exercise inflammation or for reducing body fat | |
| CN105661515B (en) | A kind of ferment lozenge for preventing three high diseases and preparation method thereof | |
| AU2019202205A1 (en) | Method to Discovery of Potential Bioactive Compounds in Herbals with Regrouping after Chromatography | |
| CN104799286B (en) | Composition for improving immunity and preparation method of composition | |
| CN102357105A (en) | Combination with effect of enhancing immunity and application thereof | |
| TWI791941B (en) | Use of a composition containing an extract of mushroom and/ or culture substrate thereof for treating drpression and emotional regulation | |
| CN108813610A (en) | A kind of saussurea involucrata composition and its application for improving immunity | |
| CN1726981A (en) | Antivirus medical broth of compound Neilupaer | |
| KR20080009695A (en) | Healthfood and pharmaceutical composition for amelioration of disease induced by metabolic disorder in cartilage | |
| EP2842433A1 (en) | Food for nutritional therapy of aids | |
| CN102823862A (en) | Composition for qi-insufficiency constitution as well as preparation method and purpose of composition | |
| CN106135878A (en) | Jilin Radix Ginseng oligopeptide purposes in preparation improves the food with sexual function improving or health food | |
| CN108542898A (en) | Purposes of the cajanin and combinations thereof in preparing medicament for treatment of depression | |
| CN1107501C (en) | Albendazole emulsion | |
| CN111166774A (en) | Composition for preventing and treating cerebral ischemia diseases and application thereof | |
| CN1602855A (en) | Application of forsythin in the process for preparing adiposis treating oral medicine and healthy food | |
| CN1296089C (en) | Zedoary injection preparation and its preparing method | |
| KR20220106158A (en) | Cannabinoids and Lycopene Anti-inflammatory Synergistic Combination | |
| Wang et al. | Efficacy of an Oral Solution Prepared from the Ultrasonic Extract of Radix dichroae roots against Eimeria tenella in Broiler Chickens | |
| CN1067270C (en) | Nutritious health care medicine | |
| CN104288236A (en) | Veterinary Shuanghuanglian oral liquid preparation processed by using probiotics and preparation method thereof | |
| CN1248715C (en) | Stomachic medication and preparation method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20230410 Address after: No. 142 Hanggui Road, Luopu County, Hotan Prefecture, Xinjiang Uygur Autonomous Region Patentee after: HETIAN UYGUR PHARMACEUTICAL Co.,Ltd. Address before: 830002 No. 9 Xinhua South Road, Xinjiang, Urumqi Patentee before: XINJIANG INSTITUTE OF MATERIA MEDICA |
|
| TR01 | Transfer of patent right |