CN1709284A - Extraction of anti-tumour active ingredient fatty acid from ground beetle - Google Patents
Extraction of anti-tumour active ingredient fatty acid from ground beetle Download PDFInfo
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- CN1709284A CN1709284A CNA2005100406637A CN200510040663A CN1709284A CN 1709284 A CN1709284 A CN 1709284A CN A2005100406637 A CNA2005100406637 A CN A2005100406637A CN 200510040663 A CN200510040663 A CN 200510040663A CN 1709284 A CN1709284 A CN 1709284A
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- fatty acid
- acid
- extraction
- eupolyphaga seu
- seu steleophaga
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Abstract
The present invention relates to an antitumor active component fatty acid separated and extracted from Chinese medicinal material wingless cockroach by adopting organic solvent and column chromatography. Said fatty acid is unsaturated fatty acid, and its main component includes 7-octadecenic acid, hexadecylic acid, 9-hexadecenoic acid and octadecane acid. Said fatty acid has the action for inhibiting several malignant tumors and has the action for resisting tumors and resisting mutation.
Description
Technical field
The present invention relates to a kind of antitumorigenic substance, particularly a kind of anti-tumor effective component fatty acid that from the Chinese medicine Eupolyphaga Seu Steleophaga, separates, extracts.
Background technology
At present, the development and the application development of anti-tumor chemotherapeutic new drug are rapid, the chemotherapeutics that particularly is taken from method developments such as natural drug is modified, synthetic is constantly asked the city, as paclitaxel, how uncommon paclitaxel, JM-216, strongly select, Vinorebine, open generally open up, medicines such as xeloda, Ali's rice tower, have certain therapeutic effect.Wherein, Chinese medicine is the natural drug treasure-house of China's uniqueness.
Before the present invention, the world of medicine has realized that the single medicinal material Eupolyphaga Seu Steleophaga has antitumor action and curative effect, external the leukaemia is had inhibitory action, but it is unclear to be which concrete composition in the Eupolyphaga Seu Steleophaga can effectively suppress tumor development on earth, also is familiar with unclear to its antitumor mechanism.The understanding majority of present stage rests on presumably, cupreol composition in the Eupolyphaga Seu Steleophaga of saying so is arranged and based on the unsaturated fatty acid of oleic acid antitumaous effect is arranged probably, the another Eupolyphaga Seu Steleophaga of saying has mutation and anti-tumor function at aspects such as the external hepatocarcinoma of treatment, gastric cancer.But these are inferred and saying all is theoretically unsound and Analysis on Mechanism, more lack experiment confirm.Thereby, just it really can the antineoplastic active ingredient can't be extracted, separate, development special for treating antitumor drug, and realize industrialization.
Summary of the invention
Purpose of the present invention just is to overcome above-mentioned defective, separates, extracts antineoplastic effective site fatty acid in the Eupolyphaga Seu Steleophaga.
Technical scheme of the present invention is: extract the anti-tumor effective component fatty acid from Eupolyphaga Seu Steleophaga, its major technique is characterised in that extracts the effective site fatty acid from Eupolyphaga Seu Steleophaga.
Further technical scheme of the present invention is: effective site is mainly unsaturated fatty acid.
Advantage of the present invention is to adopt simple method to separate, extract the fatty acid that really has antitumor action and curative effect in the Eupolyphaga Seu Steleophaga with effect, and has wide spectrum through experiment confirm and kill the tumor effect, and multiple malignant cell is had stronger inhibitory action.Preparation method is simple, and the medicine cost is low.
The specific embodiment
At first carry out organic solvent extraction:, after saponification, get fatty acid with organic solvent reflux, extract, such as dehydrated alcohol or petroleum ether.Detailed process and step:
Get 1000g, organic solvent reflux, extract, such as dehydrated alcohol or petroleum ether get extracting solution; Reclaim solvent, get crude fat; Can also get 1000g, supercritical extraction gets crude fat; Add dehydrated alcohol or methanol, KOH again, hot reflux, solvent evaporated gets solids; Water dissolution is used a small amount of extracted with diethyl ether then, and the water intaking layer adds HCL, regulates pH value to acid, and extracted with diethyl ether is got ether layer; Fling to solvent, get croude fatty acids;
Carry out column chromatography again: with silica gel etc. is adsorbent, is used for separation of fatty acids; With organic solvents such as petroleum ether, chloroform, methanol, ether, ethyl acetate, acetone and acetic acid and any ratio combination solvent is that eluant carries out chromatography, obtains the effective site fatty acid.
Analysis of components: be mainly 7-octadecenoic acid, hexadecanoic acid, palmitoleic acid, stearic acid, these four kinds of materials are with single extremely a plurality of arbitrary proportions combinations.
Except that containing above-mentioned 7-octadecenoic acid, hexadecanoic acid, palmitoleic acid, stearic acid, also contain myristic acid, 20 acid, oleic acid etc.
Cellulotoxic experiment:
Experiment in vitro
Experimental results show that the effectiveness of effective site of the present invention by the cell in vitro poison.
Experimental drug thing: effective site of the present invention
Experimental technique: adopt tetramethyl azo azoles indigo plant (MTT) development process to determine that drug level Eupolyphaga Seu Steleophaga effective site fatty acid promptly has the obvious suppression effect at the dosage of 100 μ g/ml to people's poorly differentiated adenocarcinoma JEG-3 BGC-823, colon-cancer cell strain LOVO and primary hepatocarcinoma cell strain HepG-II, suppression ratio reaches more than 80%.Promptly began in 2-4 hour to work, reach maximum effect effect and this effect after 24 hours and prolong in time and keep stable.
Claims (3)
1. extract the anti-tumor effective component fatty acid from Eupolyphaga Seu Steleophaga, it is characterized in that from Eupolyphaga Seu Steleophaga, extracting the effective site fatty acid.
2. according to claim 1 from Eupolyphaga Seu Steleophaga extraction anti-tumor effective component fatty acid, it is characterized in that effective site is mainly unsaturated fatty acid.
3. according to claim 2 from Eupolyphaga Seu Steleophaga extraction anti-tumor effective component fatty acid, it is characterized in that effective site is for containing 7-octadecenoic acid, hexadecanoic acid, palmitoleic acid, stearic acid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2005100406637A CN1709284A (en) | 2005-06-23 | 2005-06-23 | Extraction of anti-tumour active ingredient fatty acid from ground beetle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2005100406637A CN1709284A (en) | 2005-06-23 | 2005-06-23 | Extraction of anti-tumour active ingredient fatty acid from ground beetle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1709284A true CN1709284A (en) | 2005-12-21 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2005100406637A Pending CN1709284A (en) | 2005-06-23 | 2005-06-23 | Extraction of anti-tumour active ingredient fatty acid from ground beetle |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101032525B (en) * | 2007-04-10 | 2010-12-08 | 浙江省医学科学院 | Extracting active principles having extracorporeal anti-tumor function from housefly larva |
| CN103450142A (en) * | 2013-09-04 | 2013-12-18 | 浙江大学 | Chroman compound as well as extracting method and application thereof |
| CN103755547A (en) * | 2014-01-22 | 2014-04-30 | 中国科学院地质与地球物理研究所兰州油气资源研究中心 | Novel method for extracting fatty acid in natural products by utilizing column chromatography on silica gel |
| CN105982883A (en) * | 2015-02-17 | 2016-10-05 | 复旦大学 | Application of palmitic acid to preparation of drug for treating colon cancer |
-
2005
- 2005-06-23 CN CNA2005100406637A patent/CN1709284A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101032525B (en) * | 2007-04-10 | 2010-12-08 | 浙江省医学科学院 | Extracting active principles having extracorporeal anti-tumor function from housefly larva |
| CN103450142A (en) * | 2013-09-04 | 2013-12-18 | 浙江大学 | Chroman compound as well as extracting method and application thereof |
| CN103755547A (en) * | 2014-01-22 | 2014-04-30 | 中国科学院地质与地球物理研究所兰州油气资源研究中心 | Novel method for extracting fatty acid in natural products by utilizing column chromatography on silica gel |
| CN103755547B (en) * | 2014-01-22 | 2015-05-27 | 中国科学院地质与地球物理研究所兰州油气资源研究中心 | Novel method for extracting fatty acid in natural products by utilizing column chromatography on silica gel |
| CN105982883A (en) * | 2015-02-17 | 2016-10-05 | 复旦大学 | Application of palmitic acid to preparation of drug for treating colon cancer |
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