CN1709382A - Method for extracting anti-tumour active ingredient from ground beetle - Google Patents
Method for extracting anti-tumour active ingredient from ground beetle Download PDFInfo
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- CN1709382A CN1709382A CN 200510040664 CN200510040664A CN1709382A CN 1709382 A CN1709382 A CN 1709382A CN 200510040664 CN200510040664 CN 200510040664 CN 200510040664 A CN200510040664 A CN 200510040664A CN 1709382 A CN1709382 A CN 1709382A
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- acid
- eupolyphaga seu
- seu steleophaga
- fatty acid
- effective component
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Abstract
The present invention relates to a method for separating and extracting antineoplastic active region fatty acid from Chinese medicinal material wingless cockroach. Said invention adopts organic solvent to extract wingless cockroach to obtain crude fatty acid, then utilizes column chromatography to make separation so as to obtain active region fatty acid, mainly including 7-octadecenic acid, hexadecylic acid, 9-hexadecenoic acid and octadecane acid. Said wingless cockroach extract can be used for curing several malignant tumors.
Description
Technical field
The present invention relates to a kind of extracting method, particularly a kind of method of from the Chinese medicine Eupolyphaga Seu Steleophaga, separating, extracting antineoplastic effective site fatty acid.
Background technology
At present, the development and the application development of anti-tumor chemotherapeutic new drug are rapid, the chemotherapeutics that particularly is taken from method developments such as natural drug is modified, synthetic is constantly asked the city, as paclitaxel, how uncommon paclitaxel, JM-216, strongly select, Vinorebine, open generally open up, medicines such as xeloda, Ali's rice tower, have certain therapeutic effect.Wherein, Chinese medicine is the natural drug treasure-house of China's uniqueness.
Before the present invention, the world of medicine has realized that the single medicinal material Eupolyphaga Seu Steleophaga has antitumor action and curative effect, external the leukaemia is had inhibitory action, but it is unclear to be which concrete composition in the Eupolyphaga Seu Steleophaga can effectively suppress tumor development on earth, also is familiar with unclear to its antitumor mechanism.The understanding majority of present stage rests on presumably, cupreol composition in the Eupolyphaga Seu Steleophaga of saying so is arranged and antitumaous effect is probably arranged based on the unsaturated fatty acid of oleic acid, the another Eupolyphaga Seu Steleophaga of saying has mutation and anti-tumor function at aspects such as the external hepatocarcinoma of treatment, gastric cancer, but be theoretically unsound and Analysis on Mechanism, more lack experiment confirm.Thereby, just it really can the antineoplastic active ingredient can't be extracted, separate, development special for treating antitumor drug, and realize industrialization.
Summary of the invention
Purpose of the present invention just is to overcome above-mentioned defective, develops a kind of separation, extracts the method for antineoplastic active ingredient in the Eupolyphaga Seu Steleophaga.
Technical scheme of the present invention is: a kind ofly extract the method for anti-tumor effective component from Eupolyphaga Seu Steleophaga, its major technique is characterised in that Eupolyphaga Seu Steleophaga got croude fatty acids through organic solvent extraction, again through column chromatography separate the effective site fatty acid.
Further technical scheme of the present invention is: extracting anti-tumor effective component fatty acid Main Ingredients and Appearance from Eupolyphaga Seu Steleophaga is 7-octadecenoic acid, hexadecanoic acid, palmitoleic acid, stearic acid.
The fatty acid composition that advantage of the present invention and effect are to adopt simple method to separate, extract and really have antitumor action and curative effect in the Eupolyphaga Seu Steleophaga, and carry thing through experiment confirm single medicinal material Eupolyphaga Seu Steleophaga organic solvent and have wide spectrum and kill the tumor effect, can be used for treating multiple malignant tumor.
The specific embodiment
Organic solvent extraction---with organic solvent reflux, extract, such as dehydrated alcohol or petroleum ether, after saponification, get croude fatty acids; Again through column chromatography---carry out chromatography.Detailed process and step:
Get 1000g, organic solvent hot refluxs such as dehydrated alcohol or petroleum ether are extracted, and get extracting solution; Reclaim solvent, get crude fat; Can also get 1000g, supercritical extraction gets crude fat; Add dehydrated alcohol or methanol, KOH again, hot reflux, solvent evaporated gets solids; Water dissolution is used extracted with diethyl ether then, and the water intaking layer adds HCL, regulates pH value to acid, and extracted with diethyl ether is got ether layer; Fling to solvent, get croude fatty acids; Be adsorbent with silica gel etc. again, be used for separation of fatty acids; With organic solvents such as petroleum ether, chloroform, methanol, ether, ethyl acetate, acetone and acetic acid and any ratio combination solvent is that eluant carries out chromatography, obtains the effective site fatty acid.
Analysis of components: be mainly 7-octadecenoic acid, hexadecanoic acid, palmitoleic acid, stearic acid.
Cellulotoxic experiment: experiment in vitro
Experimental results show that the effectiveness of effective site of the present invention by the cell in vitro poison.
Experimental drug thing: effective site of the present invention
Experimental technique: adopt tetramethyl azo azoles indigo plant (MTT) development process to determine drug level
Eupolyphaga Seu Steleophaga effective site fatty acid promptly has the obvious suppression effect at the dosage of 100 μ g/ml to people's poorly differentiated adenocarcinoma JEG-3 BGC-823, colon-cancer cell strain LOVO and primary hepatocarcinoma cell strain HepG-II, and suppression ratio reaches more than 80%.Promptly began in 2-4 hour to work, reach maximum effect effect and this effect after 24 hours and prolong in time and keep stable.
The present invention is in research, analyzes the separation of carrying out fatty acid on the basis that the fatty acid draw in the Eupolyphaga Seu Steleophaga has antitumor action, so protection scope of the present invention is not limited in the description of the above-mentioned specific embodiment.
Claims (4)
1. one kind is extracted the method for anti-tumor effective component from Eupolyphaga Seu Steleophaga, it is characterized in that Eupolyphaga Seu Steleophaga is got croude fatty acids through organic solvent extraction, again through column chromatography separate the anti-tumor effective component fatty acid.
2. a kind of method from Eupolyphaga Seu Steleophaga extraction anti-tumor effective component according to claim 1 is characterized in that the effective site fatty acid Main Ingredients and Appearance that extracts is 7-octadecenoic acid, hexadecanoic acid, palmitoleic acid, stearic acid.
3. a kind of method from Eupolyphaga Seu Steleophaga extraction anti-tumor effective component according to claim 1 is characterized in that organic solvent hot reflux extraction, and saponification gets croude fatty acids.
4. according to claim 1ly a kind ofly extract the method for anti-tumor effective component, it is characterized in that croude fatty acids carrying out chromatography with eluant again through adsorbents adsorb from Eupolyphaga Seu Steleophaga, the effective site fatty acid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510040664 CN1709382A (en) | 2005-06-23 | 2005-06-23 | Method for extracting anti-tumour active ingredient from ground beetle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510040664 CN1709382A (en) | 2005-06-23 | 2005-06-23 | Method for extracting anti-tumour active ingredient from ground beetle |
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| Publication Number | Publication Date |
|---|---|
| CN1709382A true CN1709382A (en) | 2005-12-21 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200510040664 Pending CN1709382A (en) | 2005-06-23 | 2005-06-23 | Method for extracting anti-tumour active ingredient from ground beetle |
Country Status (1)
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| CN (1) | CN1709382A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101032525B (en) * | 2007-04-10 | 2010-12-08 | 浙江省医学科学院 | Extracting active principles having extracorporeal anti-tumor function from housefly larva |
| CN108904540A (en) * | 2018-07-18 | 2018-11-30 | 广东工业大学 | Extraction, purification process and the application of alkaloid in a kind of eupolyphoge sinensis |
-
2005
- 2005-06-23 CN CN 200510040664 patent/CN1709382A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101032525B (en) * | 2007-04-10 | 2010-12-08 | 浙江省医学科学院 | Extracting active principles having extracorporeal anti-tumor function from housefly larva |
| CN108904540A (en) * | 2018-07-18 | 2018-11-30 | 广东工业大学 | Extraction, purification process and the application of alkaloid in a kind of eupolyphoge sinensis |
| CN108904540B (en) * | 2018-07-18 | 2020-10-23 | 广东工业大学 | Extraction and purification method and application of alkaloid in ground beetle |
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