CN1689422A - Sleep assisting milk and its production method - Google Patents
Sleep assisting milk and its production method Download PDFInfo
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- CN1689422A CN1689422A CN 200410018001 CN200410018001A CN1689422A CN 1689422 A CN1689422 A CN 1689422A CN 200410018001 CN200410018001 CN 200410018001 CN 200410018001 A CN200410018001 A CN 200410018001A CN 1689422 A CN1689422 A CN 1689422A
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Abstract
The sleep-improving milk features the material comprising milk in 84-95 weight portions, whey protein in 2.5-12.5 weight portions, and alpha-lactoalbumin in 0.05-1 weight portions. The production process of the sleep-improving milk is also provided comprising the steps of adding whey protein into milk, adding alpha-lactoalbumin, stirring, stirring, homogenizing and sterilizing. The sleep-improving milk has function of improving sleep quality.
Description
Technical field
The present invention relates to a kind of milk, also relate to the manufacture method of milk, relate in particular to a kind of milk and manufacture method thereof of improving sleep function that have.
Background technology
Awakening and sleep are the necessary processes of human physiological activity.Only under wakefulness, human body just can be worked and other activities.And, can make the energy of human body and muscle power obtain replying by sleep, to keep good wakefulness.But along with factors such as rhythm of life is accelerated, in-depth, the school work of work competition are heavy cause various mental pressure and mental maladjustment, thereby causing increasing of mental sickness such as insomnia, spirit depressing division, is transient insomnia fortunately more than 90% among the insomnia crowd.According to statistics, there is U.S. of 2/3 to admit that promptly 5,000 ten thousand people have sleep disorder nearly approximately.And have numerous sleep disorder person especially in China, at present China has at least 30% its people to suffer from various degree insomnia, the insomnia rate of middle-aged and elderly people then up to 70%! The dyssomnia crowd surpasses 300,000,000.Sleep disorder has comprised the lighter such as awaken night several times, and severe patient can be lain awake all night.The most frequently used method of eliminating sleep disorder is to take sleeping pill.But take sleeping pill for a long time, will produce toleration and addiction.For a long time may occur bouncing behind clothes all standing aypnia and give up effect forms vicious cycle.Therefore people more pay close attention to by the diet orderliness, improve sleep quality, improve sleep state.At home, the sleeping health-product market is day by day ripe, and the sleeping notion is generally accepted by consumer, the quality of people's pay attention to day by day sleep and healthy relation.According to statistics, China's sleep produce market is approximately 5,000,000,000.The traditional view of " Diet cures more than the doctors " that Chinese believe in makes the sleeping preparation of natural nutrition be expected to become novel health product, wherein regulates nerve with " green natural and the food of hypnotic ", improves sleep quality and will become fashion trend.
The material of sleeping at present has
Melatonin
The melatonin formal name used at school is N-acetyl group-5-methoxytryptamine, is the synthetic and excretory a kind of indoles hormone of pineal gland body in the vertebrate brain.Its pure product are faint yellow leaflet crystal, and molecular formula is C
13H
16N
2O
2, relative molecular mass 232.27, fusing point 116-118 ℃.Circadian rhythm and hypnotic pharmacological action thereof to melatonin both at home and abroad all has roundup, and the result shows: melatonin and sleep and dyssomnias are closely related.The experiment of different animals, various dose, different indexs shows that all melatonin and sleep and dyssomnias are closely related, has the effect of tangible improvement sleep.External source is replenished melatonin, can improve sleep and rebuild sleep rhythm.Melatonin has its unique characteristics as a kind of novel hypnotic drug or food: 1) low dose just has the comparatively ideal sleeper effect that improves.2) melatonin works by the adjusting to hormonal system as a kind of endogenous material, is not foreign body concerning body.Melatonin has the metabolic pathway of self in vivo, can not cause female medicine and metabolite thereof accumulating in vivo.3) the melatonin biological half-life is short, promptly reduces to normal person's physiological level after oral several hours, can be because of not accumulating the residual energy that influences next day.4) toxicity is minimum.Zoopery can not surveyed its median lethal dose(LD 50), even also do not cause rats death when heavy dose of (800mg/kg).But take 300mg/ day melatonin continuously after 4 months, have the side effect that suppresses ovulation.Therefore the syngignoscism of melatonin has the characteristics of endogenous, low dose, efficient, low toxicity.Ministry of Public Health approved melatonin is for improving functional factor (the Ernest Hartmann M.D.et al. of sleep health food, Sleep Induced by L-Tryptophan.TheJournal of Nervous and Mental Disease, 167:497-499; Lois A.Steinberg et al., Tryptophan Intake Influences Infants Sleep Latency.J nutr 122:1781-1791; Jin Zonglian, the functional evaluation of health food and exploitation, China Light Industry Press, 2001).But the melatonin product mostly is tablet or capsule at present.
Alpha-lactalbumin
Alpha-lactalbumin is a kind of genuine milk albumen that is present in the milk, and its tryptophane is higher.The extra alpha-lactalbumin of adding makes and contains high-load tryptophan in the product in milk.And tryptophan is a kind of of essential amino acids, still is the precursor of 5-hydroxy tryptamine, the i.e. synthetic in vivo precursor of melatonin simultaneously.The biosynthetic in vivo precursor of melatonin is a tryptophan, therefore the high food of external complementary colors histidine content also can increase secretory volume (the C Rob Markus et al of melatonin, The bovine protein α-lactalbumin increase the plasma ratio of tryptophan to the other large neutralamino acids, and in vulnerable subjects raises brain serotonin activity, reducescortisol concentration, and improves mood under stress.Am J Clin Nutr2000; 71:1536-44; J.Minet-Ringuet et al., A tryptophan-rich protein dietefficiently restores sleep after food deprivation in the rat.Elsevier 2003; C RobMarkus et al., Whey protein rich in α-lactalbumin increases the ratio of plasmatryptophan to the sum of the other large neutral amino acid and improvescognitive performance in stress-vulnerable subjects.Am J Clin Nutr 2002; 75:1051-6).Yet in the experimentation of rarely seen these foreign literatures of alpha-lactalbumin hypnotic effect, it is used in the baby food at present more, and what still do not have suitability for industrialized production is the improvement sleep product of characteristic with it.
And milk is the source of high quality protein.Be rich in the calcium that easily is absorbed by the body in the milk, calcium is an important nutrient to movement human.Iodine in the milk, zinc and lecithin can improve the work efficiency of human brain; Magnesium in the milk can increase heart and neural fatigue resistance.
Can the nutrient structure of milk makes it naturally have certain sleeping effect, on this basis, can have more the effect of natural sleeping for existing milk, and have good organoleptic quality by strengthening the various nutrient substance that help sleeping; And how to guarantee that those nutrient substance are not destroyed in whole process of preparation, still await further research.
Summary of the invention
The present invention is just at the development of as above problem, one of purpose is that disclosing a kind of is carrier with milk, strengthen protein content with lactalbumin, add alpha-lactalbumin, interpolation or do not add nutrient substance such as melatonin, add or do not add emulsifying agent, thickening agent, flavor substance, the milk product of after sterilization, making; Two of purpose provides the preparation method of the above-mentioned easypro milk of sleeping.
The object of the present invention is achieved like this: a kind of milk of sleeping that relaxes is characterized in that the raw material of this milk comprises 84-95 part milk, 2.5-12.5 part lactalbumin, 0.05-1 part alpha-lactalbumin.
The milk of sleeping that relaxes of the present invention can also contain 0.05-2 part emulsifying agent+stabilizing agent, 0-0.1 part flavor substance and/or 0.0001-0.002 part melatonin.
The definition of milk among the present invention: ordinary milk or the recombined milk or the beverage containing milk that are mixed with milk powder, whey powder, rare butter or other milk ingredients.
Lactalbumin comprises: whey protein concentrate, whey isolate protein, whey powder and other are the proteinaceous whey product of raw material production with the milk surum.
Alpha-lactalbumin comprises: be raw material with milk, alpha-lactalbumin content is higher than conventional milk after treatment, and the newborn basigamy material of the stable alpha-lactalbumin content that can measure is arranged.
Emulsifying agent, thickening agent, flavor substance comprise: various all kinds of emulsifying agents, thickening agent and edible essence, the spice that allow use in GB14880, GB2760.
The preparation method of the above-mentioned easypro milk of sleeping may further comprise the steps:
1. lactalbumin adds in the milk;
2. add alpha-lactalbumin;
3. stir;
4. pass through homogenizing;
5. promptly obtain the milk of sleeping that relaxes of the present invention after the sterilization.
Wherein, this homogenizing is the one-level homogenizing, at 60-80 ℃, carries out under the 150-300bar.
Above-mentioned homogenizing can also be double-stage homogenization, and at 60-80 ℃, the first order is at 30-50bar, and carry out under 100-250bar the second level.
Above-mentioned sterilization conditions is 65-140 ℃, and the time is 3 seconds to 30 minutes.
Method of the present invention can also add emulsifying agent+stabilizing agent in step in 2., and/or flavor substance; Be preferably in this moment before the adding above-mentioned substance, promptly 1. stirred 5-10 minute the back in step earlier.
In addition, add melatonin in 2., or through 5. step is sterilized and cooled off after, add melatonin and stir in step, can be according to the adjustment of process equipment situation, wherein said chilling temperature is preferably 4-25 ℃.And the flavor substance adding technology is constant.
The following expression of the key technical indexes of the easypro milk of sleeping of the present invention:
Index:
| Project | Index |
| Fat | ????0-4％ |
| Protein | 2.9-5.0％ |
| Non-fat solid | 6.0-11.0％ |
| Alpha-lactalbumin | 120mg/100ml～1200mg/100ml |
| Melatonin | 0～20ppm |
Lactalbumin among the present invention is employed for many years as the high-nutrition food additive.The essential amino acids content height of lactalbumin, its biological value is than other meals albumen height.The content of lactalbumin sulfur-containing amino acid is higher relatively, and especially cysteine has immune activation function (U.S.Dairy ExportCouncil, U.S.'s milk surum and lactose prod reference manual, 2004).The easypro milk of sleeping of the present invention can reach the effect of natural sleeping, and existing ordinary milk more can improve sleep function.
The specific embodiment
Embodiment 1
Prescription:
| Raw material | Ingredient requirement | Addition (weight portion) |
| Milk | Fat≤0.5%, protein 〉=2.5%, non-fat solid 〉=8.1% | ????86.5 |
| Lactalbumin | Protein 〉=17% | ????12.5 |
| Alpha-lactalbumin | Alpha-lactalbumin 〉=20% | ????1 |
| Melatonin | ????0.002 |
Preparation process is as follows:
1. lactalbumin adds in the milk, stirs 5 minutes;
2. add alpha-lactalbumin and melatonin;
3. stirred 5 minutes;
4. at 60 ℃, carry out the one-level homogenizing under the 150bar;
5. sterilize down at 65 ℃ and promptly obtained the cattle milk of sleeping that relaxes of the present invention in 30 minutes; For suitability for industrialized production needs, also can
6. be cooled to 4 ℃ and carry out fill.
Assay is as follows:
Assay:
| Project | Index |
| Fat | ????0.40％ |
| Protein | ????5.00％ |
| Non-fat solid | ????11.0％ |
| Alpha-lactalbumin | ????1200mg/100ml |
| Melatonin | ????20ppm |
Embodiment 2
Prescription:
| Raw material | Ingredient requirement | Addition (weight portion) |
| Milk | Fat 〉=1.0%, protein 〉=1.5%, non-fat solid 〉=6.0% | ?94.35 |
| Lactalbumin | Protein 〉=17% | ?5.0 |
| Alpha-lactalbumin | Lactalbumin 〉=20% | ?0.05 |
| Emulsifying agent, thickening agent | ?0.5 | |
| Orange flavor | ?0.1 |
Preparation process is as follows:
1. lactalbumin adds in the milk, stirs 6 minutes;
2. add alpha-lactalbumin, emulsifying agent, thickening agent and orange flavor;
3. stirred 7 minutes;
4. at 75 ℃, carry out double-stage homogenization under the 30/100bar;
5. sterilize down at 120 ℃ and promptly obtain the cattle milk of sleeping that relaxes of the present invention 10 seconds; For suitability for industrialized production needs, also can
6. be cooled to 10 ℃ and carry out fill.
Assay is as follows:
Assay:
| Project | Index |
| Fat | ????1.0％ |
| Protein | ????2.9％ |
| Non-fat solid | ????6.0％ |
| Alpha-lactalbumin | ????120mg/100ml |
Embodiment 3
Prescription:
| Raw material | Ingredient requirement | Addition (weight portion) |
| Milk | Fat 〉=3.8%, protein 〉=1.4%, non-fat solid 〉=7.1% | ?87.85 |
| Lactalbumin | Protein 〉=17% | ?9.6 |
| Alpha-lactalbumin | Alpha-lactalbumin 〉=20% | ?0.5 |
| Melatonin | ?0.0001 | |
| Emulsifying agent, thickening agent | ?2 | |
| Strawberry essence | ?0.05 |
Preparation process is as follows:
1. lactalbumin adds in the milk, stirs 7 minutes;
2. add alpha-lactalbumin, emulsifying agent, thickening agent and strawberry essence;
3. stirred 5 minutes;
4. at 70 ℃, carry out the one-level homogenizing under the 300bar;
5. sterilized for 30 seconds down at 110 ℃;
6. be cooled to 15 ℃, add the melatonin restir and promptly obtained the milk of sleeping that relaxes of the present invention in 8 minutes.
Assay is as follows:
Assay:
| Project | Index |
| Fat | ????4.0％ |
| Protein | ????3.5％ |
| Non-fat solid | ????7.0％ |
| Alpha-lactalbumin | ????800mg/100ml |
| Melatonin | ????1ppm |
Embodiment 4
Prescription:
| Raw material | Ingredient requirement | Addition (weight portion) |
| Milk | Fat 〉=3.0%, protein 〉=1.0%, non-fat solid＞8.5% | ?92.3 |
| Lactalbumin | Protein 〉=23% | ?7.5 |
| Alpha-lactalbumin | Alpha-lactalbumin 〉=20% | ?0.2 |
Preparation process is as follows:
1. lactalbumin adds in the milk;
2. add alpha-lactalbumin;
3. stirred 10 minutes;
4. at 80 ℃, carry out the one-level homogenizing under the 220bar;
5. sterilize down at 95 ℃ and promptly obtained the milk of sleeping that relaxes of the present invention in 5 minutes.
Assay is as follows:
Assay:
| Project | Index |
| Fat | ????3.0％ |
| Protein | ????4.0％ |
| Non-fat solid | ????9.0％ |
| Alpha-lactalbumin | ????300mg/10ml |
Embodiment 5
Prescription:
| Raw material | Ingredient requirement | Addition (weight portion) |
| Milk | Fat 〉=1.8%, protein 〉=1.7%, non-fat solid 〉=9.5% | ?92.65 |
| Lactalbumin | Protein 〉=34% | ?6.5 |
| Alpha-lactalbumin | Alpha-lactalbumin 〉=20% | ?0.8 |
| Melatonin | ?0.001 | |
| Flavoring pineapple essence | ?0.05 |
Preparation process is as follows:
1. lactalbumin adds in the milk, stirs 9 minutes;
2. add alpha-lactalbumin and flavoring pineapple essence;
3. stirred 9 minutes;
4. at 70 ℃, carry out double-stage homogenization under the 40/150bar;
5. sterilized 16 seconds down at 85 ℃;
6. be cooled to 25 ℃, stir behind the adding melatonin and promptly obtained the milk of sleeping that relaxes of the present invention in 6 minutes.
Assay is as follows:
Testing result:
| Project | Index |
| Fat | ????2.0％ |
| Protein | ????4.5％ |
| Non-fat solid | ????10.0％ |
| Alpha-lactalbumin | ????1000mg/100ml |
| Melatonin | ????10ppm |
Embodiment 6
Prescription:
| Raw material | Ingredient requirement | Addition (weight portion) |
| Milk | Fat 〉=1.0%, protein 〉=1.6%, non-fat solid 〉=8.0% | ??93.0 |
| Lactalbumin | Protein 〉=34% | ??2.5 |
| Alpha-lactalbumin | Alpha-lactalbumin 〉=20% | ??0.3 |
| Emulsifying agent, thickening agent | ??1 |
Preparation process is as follows:
1. lactalbumin, alpha-lactalbumin and emulsifying agent, thickening agent are added in the milk, stirred 7 minutes;
2. at 75 ℃, carry out double-stage homogenization under the 50/250bar;
3. sterilize down at 100 ℃ and promptly obtained the cattle milk of sleeping that relaxes of the present invention in 15 seconds; For suitability for industrialized production needs, also can
4. be cooled to 10 ℃ and carry out fill.
Assay is as follows:
Assay:
| Project | Index |
| Fat | ????1.5％ |
| Protein | ????4.0％ |
| Non-fat solid | ????8.0％ |
| Alpha-lactalbumin | ????500mg/100ml |
The subjective appreciation result of the easypro milk of sleeping of effect embodiment 1 the present invention
With embodiment 4 is example, the results are shown in following table:
| Index | Milk | The milk of sleeping relaxes |
| Milk | ????36 | ????54 | |
| Mouthfeel | ????40 | ????50 | |
| Color and luster | ????34 | ????50 | |
| Overall assessment (person-time) | Excellent | ????0 | ????16 |
| Very | ????8 | ????2 | |
| Generally | ????12 | ????2 | |
Illustrate: the subjective appreciation experiment is finished by 20 valuation officers.The high person of score is then effective, estimates high.
By above data show, relaxing sleeps suckles has bigger improvement than ordinary milk on local flavor, and it is obvious to improve sleeper effect through the zoopery proof.
The functional characteristic evaluation that effect embodiment 2 the present invention are relaxed and slept and suckle
Improve the method for inspection of sleep effect and select other adults of healthy unicity for use, recommend to use BALB/c mouse.Righting reflex loss with mice is the sleep index.When mice places supine position, can right the body position immediately, can not the person of righting as surpassing 30～60s, promptly think righting reflex loss to enter sleep.Right emission and recover to be the animal awakening.Righting emission disappears to recovering to be during this period of time the animal sleep time.Observe and tried whether mice peace and quiet occur behind the thing, close order, drowsiness or sleep phenomenon, and use mechanism of action clear and definite, typical central depressant thing is as instrument, observe to be tried thing and whether known drug has synergism.Usually select barbiturates tranquilizers such as pentobarbital sodium, barbital sodium for use.
1, directly sleep test
2, prolong the pentobarbital sodium test length of one's sleep
3, pentobarbital sodium (or barbital sodium) subliminal hypnosis experiment
4, barbital sodium sleep experiment incubation period
In sum, the functional evaluation zoopery project of improving the sleep product mainly comprises: the length of one's sleep, sleep incidence rate, autonomic activities (being above-mentioned experiment 2,3,4), two indexs are positive in three indexs of zoopery can judge that then this sample has the function (list of references: Shao person of outstanding talent that improves sleep, the health food engineering, Hunan science tech publishing house, 2001.Jin Zonglian, the functional evaluation of health food and exploitation, China Light Industry Press, 2001).
(1) material and method
1. embodiment 4 samples
Milky white liquid, the 250ml/ bag, the human body recommended dose is 250ml/ days.
2. laboratory animal
The BALB/c1 monthly age that this experiment selects for use Chinese Academy of Medical Sciences's animal center breeding field to provide male secondary mice, body weight 18～22g is divided into 4 groups at random by body weight, body weight is through t check no significant difference (p＞0.05) between group.
3. dosage is selected
Tried thing human body recommended dose and be 250ml/ days approved product, the dose,equivalent of mice is equivalent to 10 times of human body recommended dose, respectively with 1.67 times, 3.33 times, 10 times of human body recommended dose as basic, normal, high dosage.Take administration by gavage, matched group is irritated common whole milk, and each dosage group gives various dose respectively and tried thing.Each organize mice tried thing once after, measure every index.
4. instrument and reagent
AE100 electronic balance, DT500 electronic balance, stopwatch, pentobarbital sodium, barbital sodium, normal saline.
5. detection method
(1) prolongs the inductive mouse sleep time test of pentobarbital sodium
Animal is tried thing after 26 days continuously, gives each treated animal lumbar injection 30mg/kg body weight pentobarbital sodium, and injection volume is the 0.1ml/10g body weight.With the mice righting reflex loss is the sleep index, observes to be tried thing and whether prolong pentobarbital sodium inductive length of one's sleep.Test was carried out at night.
(2) sub-threshold dose pentobarbital sodium sleep incidence rate test
Animal is tried thing after 26 days continuously, gives each treated animal lumbar injection 26mg/kg body weight pentobarbital sodium, and injection volume is the 0.1ml/10g body weight.Reaching the above person of 1min with the mice righting reflex loss is sleeping standard, sleeping number of animals in the record 30min.Test was carried out at night.Can observation is tried thing improve sleep incidence rate under the pentobarbital sodium threshold.
(3) barbital sodium sleep latency test
Animal is tried thing after 26 days continuously, gives each treated animal lumbar injection 240mg/kg body weight barbital sodium, and injection volume is the 0.1mg/10g body weight.With the mice righting reflex loss is the sleep index, observes and is tried thing to preclinical influence of barbital sodium sleep.
6. Data Processing in Experiment
Experimental data adopts variance analysis and x
2Check is analyzed.
(2) result
1. tried the influence of thing to the normal mouse body weight
By table 1 as seen, compare with matched group, each dosage group body weight does not all have significant difference (p＞0.05).Therefore being tried thing does not influence the mice body weight.
| Table 1 relaxes and to sleep milk to the influence of normal mouse body weight (x ± SD) | |||||
| Group | ???n | Body weight (g) before the experiment | The P value | Experiment back body weight (g) | The P value |
| Matched group | ???15 | ????18.1±1.1 | ??/ | ????19.4±1.9 | ?/ |
| Low dose group | ???15 | ????17.4±0.9 | ??0.0847 | ????18.4±1.4 | ?0.1226 |
| Middle dosage group | ???15 | ????17.8±0.9 | ??0.4925 | ????18.4±0.9 | ?0.0845 |
| High dose group | ???15 | ????17.6±0.8 | ??0.1554 | ????18.5±0.9 | ?0.1200 |
2. tried thing to the influence of the length of one's sleep of pentobarbital sodium inducing mouse
By table 2 as seen, what per os gave the mice various dose was tried thing after 26 days, compare with matched group, the basic, normal, high dosage group pentobarbital sodium induced hypnotic time is improved 116% (p＜0.001), 135% (p＜0.001) and 123% (p＜0.001) respectively, shows to be tried thing and the threshold dose pentobarbital sodium has collaborative sleep effect.
| Table 2 relaxes and to sleep milk to the pentobarbital sodium inducing mouse influence of the length of one's sleep (x ± SD) | |||
| Group | ????n | The length of one's sleep (min) | The P value |
| The blank group | ????15 | ??26.0±17.9 | ????/ |
| Low dose group | ????15 | ??56.2±22.4 *** | ????3.390E-04 |
| Middle dosage group | ????15 | ??61.1±21.2 *** | ????3.701E-05 |
| High dose group | ???15 | ??58.1±21.5 *** | ????1.272E-04 |
* *With matched group utmost point significant difference (p＜0.001) is arranged relatively.
3. tried the influence of thing to sub-threshold dose pentobarbital sodium inducing mouse sleep incidence rate
By table 3 as seen, what per os gave the mice various dose was tried thing after 26 days, compare with matched group, basic, normal, high dosage group sub-threshold dose pentobarbital sodium induced hypnotic incidence rate improves 265% (p＜0.05), 365% (p＜0.01) and 365% (p＜0.01), shows to be tried the incidence rate that thing can increase the sleep of sub-threshold dose pentobarbital sodium inducing mouse.
| Table 3 relaxes and to sleep milk to the influence of sub-threshold dose pentobarbital sodium inducing mouse sleep incidence rate (x ± SD) | |||
| Group | ????n | The sleep incidence rate | The P value |
| The blank group | ????15 | ????20％ | ????/ |
| Low dose group | ????15 | ????73％ * | ????0.0159 |
| Middle dosage group | ????15 | ????93％ ** | ????0.0033 |
| High dose group | ????15 | ????93％ ** | ????0.0033 |
*Relatively there were significant differences (p＜0.05) with matched group
*With matched group highly significant difference (p＜0.01) is arranged relatively
4. tried thing to preclinical influence of barbital sodium inducing mouse sleep
By table 4 as seen, what per os gave the mice various dose was tried thing after 26 days, compare with matched group, basic, normal, high dosage group barbital sodium inducing mouse is slept and is shortened 13% (p＜0.05), 30% (p＜0.001), 22% (p＜0.001) incubation period, shows and is tried the incubation period that thing can prolong the barbital sodium induced hypnotic.
| Table 4 relaxes and to sleep milk to the barbital sodium inducing mouse preclinical influence (x ± SD) that sleeps | |||
| Group | ????n | Sleep incubation period (min) | The p value |
| The blank group | ????15 | ????30.7±4.0 | ????/ |
| Low dose group | ????15 | ????26.8±4.3 * | ????0.0166 |
| Middle dosage group | ????15 | ????21.6±3.3 *** | ????2.327E-07 |
| High dose group | ????15 | ????24.0±3.7 *** | ????5.789E-05 |
*Relatively there were significant differences (p＜0.05) with matched group
* *With matched group utmost point significant difference (p＜0.001) is arranged relatively.
Raw material sources in the embodiment of the invention:
Milk: Shanghai Bright Dairy ﹠ Food Co., Ltd the 7th, nine pastures.
Lactalbumin: New Zealand's dairy starting material (China) company limited, Halib food company (U.S.), PROLIANT company (U.S.)
Alpha-lactalbumin: Dai Weilin trade (Shanghai) Co., Ltd., Glanbia batching company (U.S.), Arla food company (Denmark)
Melatonin: the scientific and technological Food Co., Ltd in city far away
In this description said percentage when umber all be weight percentage and weight portion.
Claims (10)
1, a kind of milk of sleeping that relaxes is characterized in that the raw material of this milk comprises that weight portion is the milk of 84-95 part, the lactalbumin of 2.5-12.5 part, the alpha-lactalbumin of 0.05-1 part.
2, the milk of sleeping that relaxes according to claim 1 is characterized in that also containing 0.05-2 part emulsifying agent and stabilizing agent, 0-0.1 part flavor substance and/or 0.0001-0.002 part melatonin.
3, the preparation method of the easypro milk of sleeping as claimed in claim 1 is characterized in that this method may further comprise the steps:
1. lactalbumin adds in the milk;
2. add alpha-lactalbumin;
3. stir;
4. pass through homogenizing;
5. promptly obtain the milk of sleeping that relaxes of the present invention after the sterilization.
4, method as claimed in claim 3 is characterized in that this homogenizing is the one-level homogenizing, at 60-80 ℃, carries out under the 150-300bar.
5, method as claimed in claim 3 is characterized in that this homogenizing is a double-stage homogenization, and at 60-80 ℃, the first order is at 30-50bar, and carry out under 100-250bar the second level.
6, method as claimed in claim 3 is characterized in that this sterilization conditions is 65-140 ℃, and the time is 3 seconds to 30 minutes.
7, method as claimed in claim 3 is characterized in that also adding emulsifying agent and stabilizing agent in step in 2., and/or flavor substance.
8, method as claimed in claim 7 is characterized in that 1. stirring 5-10 minute the back in step earlier.
9,, it is characterized in that adding melatonin in 2. in step as the described method of the arbitrary claim of claim 3-8; Or after 5. step is sterilized and cooled off, add melatonin, promptly obtain the milk of sleeping that relaxes of the present invention behind the restir.
10, method as claimed in claim 9 is characterized in that described chilling temperature is 4-25 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410018001 CN1689422A (en) | 2004-04-28 | 2004-04-28 | Sleep assisting milk and its production method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410018001 CN1689422A (en) | 2004-04-28 | 2004-04-28 | Sleep assisting milk and its production method |
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| Publication Number | Publication Date |
|---|---|
| CN1689422A true CN1689422A (en) | 2005-11-02 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200410018001 Pending CN1689422A (en) | 2004-04-28 | 2004-04-28 | Sleep assisting milk and its production method |
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| Country | Link |
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| CN (1) | CN1689422A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101248816B (en) * | 2008-03-28 | 2010-07-21 | 新希望乳业控股有限公司 | Low lactose pasteurizing sterilizing milk and preparation thereof |
| CN103327826A (en) * | 2010-12-10 | 2013-09-25 | 脱其泰有限责任公司 | Food composition for hemophagous insects |
| WO2013068565A3 (en) * | 2011-11-10 | 2014-05-01 | Eratech S.R.L. | Melatonin-based solutions and powders for their preparation |
| CN105941629A (en) * | 2016-06-12 | 2016-09-21 | 河北三元食品有限公司 | Pressure-reducing sleep-promoting milk-containing functional beverage capable of reducing stress negative reactions of white-collar workers and students |
| CN107467647A (en) * | 2016-06-07 | 2017-12-15 | 内蒙古伊利实业集团股份有限公司 | It is a kind of that there is the functional composition for improving sleep and its application |
| CN107771945A (en) * | 2017-10-28 | 2018-03-09 | 卜球 | A kind of formula milk and its manufacture method for helping to improve middle-aged and old sleep qualities |
| CN110801015A (en) * | 2019-10-25 | 2020-02-18 | 运鸿集团股份有限公司 | Walnut, perilla and fish oil capsule with brain strengthening, nerve calming and memory enhancing functions and preparation method thereof |
-
2004
- 2004-04-28 CN CN 200410018001 patent/CN1689422A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101248816B (en) * | 2008-03-28 | 2010-07-21 | 新希望乳业控股有限公司 | Low lactose pasteurizing sterilizing milk and preparation thereof |
| CN103327826A (en) * | 2010-12-10 | 2013-09-25 | 脱其泰有限责任公司 | Food composition for hemophagous insects |
| WO2013068565A3 (en) * | 2011-11-10 | 2014-05-01 | Eratech S.R.L. | Melatonin-based solutions and powders for their preparation |
| JP2014533251A (en) * | 2011-11-10 | 2014-12-11 | エラテック エス．アール．エル．Ｅｒａｔｅｃｈ Ｓ．Ｒ．Ｌ． | Melatonin-based solutions and powders for their production |
| CN107467647A (en) * | 2016-06-07 | 2017-12-15 | 内蒙古伊利实业集团股份有限公司 | It is a kind of that there is the functional composition for improving sleep and its application |
| CN107467647B (en) * | 2016-06-07 | 2022-07-15 | 内蒙古伊利实业集团股份有限公司 | Functional composition capable of improving sleep and application thereof |
| CN105941629A (en) * | 2016-06-12 | 2016-09-21 | 河北三元食品有限公司 | Pressure-reducing sleep-promoting milk-containing functional beverage capable of reducing stress negative reactions of white-collar workers and students |
| CN107771945A (en) * | 2017-10-28 | 2018-03-09 | 卜球 | A kind of formula milk and its manufacture method for helping to improve middle-aged and old sleep qualities |
| CN110801015A (en) * | 2019-10-25 | 2020-02-18 | 运鸿集团股份有限公司 | Walnut, perilla and fish oil capsule with brain strengthening, nerve calming and memory enhancing functions and preparation method thereof |
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