CN1683362A - Method for preparing soybean isoflavone, soybean spanin, oligosaccharide and soybean concentrated protein - Google Patents
Method for preparing soybean isoflavone, soybean spanin, oligosaccharide and soybean concentrated protein Download PDFInfo
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Abstract
The present invention discloses the method of extracting active functional soybean factors, including soybean isoflavone, soybean spanin, soybean oligosaccharide and functional concentrated soybean protein, from defatted soybean residue. Specially, the process of the present invention includes extracting defatted soybean residue with edible alcohol solution to obtain extracted liquid, concentration and alcohol precipitation of the extracted liquid, and conventional refining process to obtain high purity soybean isoflavone, soybean spanin, soybean oligosaccharide and functional concentrated soybean protein. The present invention has product safety, simple technological process, raised product quality and low production cost, and is suitable for industrial production.
Description
Technical field
The present invention relates to prepare the method for soybean isoflavones, soybean saponin, soybean oligosaccharide and soybean protein concentrate.More specifically, the present invention relates to from the skimmed soy beans product, extract the various active functional factor simultaneously, comprise the method for soybean isoflavones, soybean saponin, soybean oligosaccharide and soybean protein concentrate.Simple, at different levels product of this method technology and extraction solvent all can fully utilize, and are a kind of practical techniques that is applicable to large-scale industrialized production soybean isoflavones, soybean saponin, soybean oligosaccharide and soybean protein concentrate.
Background technology
Show that according to data soybean isoflavones has different physiological roles, can combine, oestrogenic hormon is played dual regulation, prevent and treat osteoporosis, prevent and treat climacteric syndrome, safety and having no side effect with estrogen receptor; Also have the provide protection to cardiovascular system of human body, can reduce the low-density lipoprotein in the blood of human body, free radical delays body aging etc. in the quencher human body, therefore can improve people's physique, improves the quality of living.At present found that the isoflavonoid of occurring in nature focuses mostly in the plant of the butterfly-shaped colored subfamily of pulse family, not only isoflavone content height but also component are many in the soybean, wherein are rich in biological value than higher Genistoside etc.
In addition, also contain multiple active function composition in the soybean, for example soybean saponin, soybean oligosaccharide and soybean protein concentrate etc. to the human body beneficial.Wherein soybean saponin (Soyasaponins) is a biologically active factors, and its chemical structure is a pentacyclic triterpene, belongs to triterpenes oleanolic acid type saponin(e, the mixture of being made up of a series of materials.Domestic and international research is verified, and soybean saponin has that the oxidation of lipotropism matter, Green Tea Extract, enhancing immunity are regulated, different physiological roles such as antitumor, antiviral.Food, medicine, cosmetic field have been applied at present.Because it has different physiological roles, has bigger potentiality and wide prospect.
Soybean protein concentrate is the soybean protein powder of protein content about 70%, main as foodstuff additive, because of it contains soy-protein, be the most economical the most economical source of human protein's nutrient substance, have certain functional performance because of soybean protein concentrate again, water conservation is arranged, protect oiliness, gelation, solvability etc. such as it, therefore may be used in the sausage food.
Soybean oligosaccharide is the alpha-galactose-glycoside class, mainly is made up of stachyose tetrose, raffinose and verbascose etc.Soybean after the maturation contains 10% oligose approximately.Soybean oligosaccharide is a kind of low sugariness, sweeting agent low in calories, and its sugariness is 70% of a sucrose, and its heat is every gram 8.36 KJ (kilojoule), only is 1/2 of sucrose heat energy, and safety non-toxic.Its nourishing function mainly is the clean intestines of defaecation, promotes bifidus bacillus propagation in the enteron aisle.Therefore, oligose can be treated constipation, various intestinal tract disease, reconciles human body intestinal microecology balance.In addition, soybean oligosaccharide also has the serum cholesterol of reduction and liver-protective effect.
In view of the excellent function of these soybean prods, hope can obtain all functional factors in the soybean simultaneously with low cost.For example the applicant discloses at patent application CN02109533.7 and extracted isoflavones, saponin, oligose and proteic method simultaneously from de-fatted soybean dregs, adopted solvent extraction, then obtain oligose by column chromatography and ultra-filtration membrane isolation technique, obtain soybean isoflavones and saponin by gradient elution and recrystallization technology, by using multiple production technology, realized the complete separation and Extraction of active substance in the dregs of beans.But still there is complex process in this technology, and the cost problem of higher particularly also needs to adopt the technology of resin absorption in the preparation of soybean isoflavones.
The inventor develops a kind of suitability for industrialized production that is suitable on the basis of above-mentioned research, produce improving one's methods of soybean isoflavones, soybean saponin, soybean oligosaccharide and functional soybean protein concentrate simultaneously with low-cost, high yield and high purity.Method of the present invention adopts food grade ethanol, and having got rid of influences the unfavorable factor that product is used for food; Adopt the alcohol precipitation washing process simultaneously, simplified production technique, saved facility investment, reduced cost; The highly purified product that the inventive method can high yield obtains, wherein the purity of soybean isoflavones can be up to 94%.Therefore method of the present invention is fit to the multiple soybean active functional factor of large-scale industrial production very much.
Summary of the invention
The purpose of this invention is to provide a kind of suitability for industrialized production that is suitable for, the method for producing soybean active functional factors such as soybean isoflavones, soybean saponin, soybean oligosaccharide and functional soybean protein concentrate simultaneously with high yield and high purity.
Particularly, method of the present invention adopts the alcohol of high density that soybean extract is carried out alcohol precipitation, and the soybean oligosaccharide in the extracting solution system is carried out initial gross separation.
Term used herein " alcohol precipitation " is meant the technological process that as precipitation agent a certain composition in the solution system is precipitated out with alcohol.
Method of the present invention adopts food grade alcohol, particularly ethanol to carry out the extraction of raw material, the processing steps such as alcohol precipitation of extracting solution, meets the requirement of food to guarantee product.
Method of the present invention also comprises recovery of employed solvent such as ethanol and recycle in the system.
More specifically, the present invention's method of preparing soybean isoflavones, soybean saponin, oligose and soybean protein concentrate may further comprise the steps:
(1) skimmed soy beans such as defatted soybean meal or the soybean germ ethanolic soln with 40-80% is extracted, obtain the dregs of beans after extraction liquid and the extraction after the filtration;
(2) described extraction liquid separates with ultra-filtration membrane with microfiltration membrane, obtains trapped fluid and sees through liquid;
(3) describedly see through concentrated solution that liquid obtains after evaporation concentration (1: 4-6) alcohol that is blended into high density carries out alcohol precipitation and handles by volume, described alcohol can be lower primary alcohols, for example methyl alcohol, ethanol, Virahol, propyl carbinol etc., preferred alcohol, the ethanol that comprises dehydrated alcohol or 95%, more preferably 95% ethanol;
(4) alcohol precipitation is handled the beds of precipitation obtain and is washed with water, and the upper strata liquid that centrifugation obtains obtains the soybean oligosaccharide syrup after decolouring, concentrating, and its further processing can be obtained the soybean oligosaccharide powder;
(5) alcohol precipitation is handled the upper strata liquid obtain add the water centrifugation after ethanol is removed in evaporation, obtains supernatant liquor and throw out, and described throw out obtains high-purity soybean isoflavone through aftertreatment;
(6) supernatant liquor that obtains of above-mentioned steps carries out fractionation by adsorption with Semi-polarity or polar macroporous resin, obtains soybean saponin;
(7) dregs of beans after the extraction that obtains after the first step extraction is filtered is through washing with alcohol, and evaporation obtains soybean protein concentrate (nitrogen soluble index NSI is about 5-10%) after removing and desolvating; Described soybean protein concentrate adds water and is modulated into the soybean protein breast, after optionally carrying out heat treated, handle with neutral proteolytic enzyme, obtain functional soybean protein concentrate through concentrated, spraying drying again, its protein content 〉=70%, nitrogen soluble index NSI 〉=80%
(8) the resulting trapped fluid of step (2) obtains soybean protein milk sap after reclaiming ethanol, and this soybean protein milk sap and step (7) are prepared the soybean protein concentrate breast merging that soybean protein concentrate technology is modulated into, and is used to prepare functional soybean protein concentrate.
The employed raw material of the inventive method is any skimmed prod of soybean, for example de-fatted soybean dregs or soybean embryo.
Extraction step described in the inventive method can be that intermittent type carries out, and also can carry out in a continuous manner.When described extraction step is carried out continuously, can adopt counter current continuous extraction equipment, carry out with the mode of counter-current extraction.Even solvent enters from staying, the raw material dregs of beans is carried by auger from the bottom up, and extraction liquid flows out in the lower end, and the dregs of beans after the extraction is discharged from top.When extraction step is wherein carried out with intermittent mode, a plurality of extractor serial or parallel connections can be connected, its extracting process can adopt the extraction adverse current, or the mode that combines with forward extraction of adverse current, and the selection that extracts mode is a purpose to reach quick mass transfer effect.No matter adopt the sort of extraction equipment and extraction mode, extraction step is carried out under temperature 40-65 ℃ usually, and the extraction time is usually more than 3 hours, for example 3-6 hour.
Extraction liquid of the present invention after filtration, the extracting solution that obtains after concentrating carries out alcohol precipitation with the alcohol of high density.The purpose of the present invention's " alcohol precipitation " step is with the oligose in the alcohol precipitation extracting solution of high density.Thereby simplified whole process flow.Preferably extracting solution being concentrated into pol in the enrichment step that is carried out before the alcohol precipitation step is 15-35 °.So-called " high density " alcohol is meant after adding this alcohol, and the determining alcohol in the system is reached more than 80%, and working concentration is at the alcohol more than 95% or 95% usually.Described alcohol is lower primary alcohols normally, for example has the primary alconol of 1-4 carbon atom, as methyl alcohol, ethanol, Virahol, propyl carbinol etc., and preferred edible alcohol, as ethanol, particularly 95% ethanol or dehydrated alcohol, most preferably 95% ethanol.The alcohol precipitation step normally stirs for some time earlier, again standing demix.Stir in the alcohol precipitation process is in order to make alcohol and material reach homogeneous, to be beneficial to obtain floss.Therefore the stirring velocity of alcohol precipitation process is not crucial, but stirring velocity should not be too fast, for example stirring velocity can be set at 20-60rpm, and churning time is 20-60min.And then left standstill maturation 2-6 hour in room temperature, described floss thoroughly is precipitated out, layering becomes the upper strata liquid and the beds of precipitation.
The supernatant liquor that obtains behind the alcohol precipitation is through concentrating, washing, centrifugation separates the throw out of back acquisition and handles through conventional method, obtains the soybean isoflavones powder after for example vacuum-drying, pulverizing, the screening, the purity of this isoflavones can reach more than 90%, is preferably more than 94%.
Obtaining that method obtains, nitrogen soluble index NSI with the present invention is that soybean protein concentrate about 5-10% can carry out heat denatured and handles or do not carry out heat denatured and handle, and carries out enzymic hydrolysis then, to obtain highly purified functional soybean protein concentrate.Wherein said heat denatured need be heated to more than 85 ℃ usually, is cooled to then about 45 ℃.Described enzymic hydrolysis is to handle with neutral protease, and described treatment process is to add neutral protease by enzyme-to-substrate than 1500-5000U/g, as papoid, bromeline, 1398 proteolytic enzyme etc., is 45-65 ℃ of reaction 3-6 hour in temperature.
Carry out separation and purification with Semi-polarity or polar macroporous resin when adopting the inventive method to prepare soybean saponin.Wherein said Semi-polarity or polar macroporous resin can be the resins of phenylethylene or oleic series, and for example, the operable polar macroporous resin such as the U.S. produce AmberliteXAD-6, the precious grace chemical industry in Cangzhou company limited produces HPD-300 etc.; The operable Semi-polarity macroporous resin such as the U.S. produce Amberlite XAD-10, Chemical Plant of Nankai Univ. produces ADS-15, AB-8 etc.
Employed water in the inventive method is that for example pure water, distilled water or reverse osmosis water as process water, are considered the cost problem, preferably use reverse osmosis water through the water of purifying in various degree.
The quality of one of product of the present invention soybean protein concentrate can its protein content and nitrogen soluble index NSI control, and so-called nitrogen soluble index is meant that water-soluble nitrogen accounts for the percentage of total nitrogen, and the solubility of the high more explanation product of nitrogen soluble index is good more, and quality is high more.
More specifically, the present invention's method of extracting the soybean active functional factor may further comprise the steps:
1, preparation high-purity soybean isoflavone:
(1) be raw material with de-fatted soybean dregs or soybean embryo, extract as solvent that its solid-liquid ratio is 1 with the 40-80% ethanolic soln: 4-10, extraction temperature is 40-65 ℃, the extraction time is 3-6 hour;
(2) extraction liquid is by coarse filtration, obtain seeing through liquid and trapped fluid through smart filtration of microfiltration membrane and ultra-filtration membrane; To concentrate evaporation simultaneously through liquid reclaims and obtains extracting solution behind the ethanol:
(3) this extracting solution being concentrated into pol is 15-35 °, and by volume 1: 4-6 adds 95% ethanol to carry out alcohol precipitation and handle, and stirs 20-60min at 20-60rpm, leaves standstill then maturation 2-6 hour, obtains supernatant liquor and throw out;
(4) above-mentioned supernatant liquor reclaims ethanol through evaporation concentration, obtains concentrated solution, and by volume 1: 4-6 adds entry, stirs 30min at 20-60rpm, in the 2000-4000rpm centrifugation, obtains throw out and supernatant liquor;
(5) above-mentioned throw out is pulverized the back screening and is obtained the soybean isoflavones powder through the negative-pressure vacuum drying, and the purity of isoflavones is more than 90%;
2, preparation soybean oligosaccharide:
The beds of precipitation that above-mentioned steps (3) is obtained stir (20-60rpm) 20-30min with 2-6 times of water washing, obtain the soybean oligosaccharide syrup through activated carbon decolorizing, vacuum concentration, and total pol is greater than 75%.
Can obtain the soybean oligosaccharide powder after the drying and crushing.
3, preparation soybean saponin:
(1) the resulting supernatant liquor of step (4) with above-mentioned preparation soybean isoflavones carries out fractionation by adsorption with Semi-polarity or polar macroporous resin, the beds of precipitation that the step (3) of the effluent liquid that is not adsorbed and 1, preparation high-purity soybean isoflavone obtains merge, and are used to prepare soybean oligosaccharide;
(2) ethanolic soln with 50-60% carries out desorb, obtains stripping liquid;
(3) this stripping liquid reclaims ethanol through evaporation process, and the concentrated solution that obtains carries out spraying drying and obtains the soybean saponin powder, and purity is greater than 75%.
4, preparation functional soybean protein concentrate:
(1) de-fatted soybean dregs after extraction, 90% ethanolic soln with solid-liquid ratio 1: 2-6 washs, washings that obtains and extraction liquid merge, soybean meal after the extraction is through carrying out precipitation treatment, carry out negative pressure drying, pulverizing, screening again and obtain soybean protein concentrate, its nitrogen soluble index is about 5-10%;
(2) above-mentioned soybean protein concentrate water is modulated into the soybean protein breast by solid-liquid ratio 1: 8-12, after heating more than 85 ℃ makes the soybean protein sex change, is cooled to 45 ℃, carries out enzymic hydrolysis again, but also can directly carry out enzymic hydrolysis without heat denatured.Enzyme hydrolysis step is pressed enzyme-to-substrate than 1: 1500-5000U/g adds neutral protease to described soybean protein concentrate Ruzhong, and for example papoid, bromeline or 1398 proteolytic enzyme etc. were at temperature 45-65 ℃ of following stirring reaction 4-6 hour;
(3) mode with heating makes enzyme deactivation in the hydrolyzed solution, obtains the soy proteins breast; It is concentrated, spraying drying obtains functional soybean protein concentrate, its protein content 〉=70%, NSI 〉=80%.
Method of the present invention has the following advantages:
(1) introduces the alcohol precipitation step, simplified production technique, particularly simplified the production technique of soybean isoflavones, improved product purity;
(2) obtain multiple product simultaneously, raw material is fully used;
(3) use edible alcohol, particularly ethanol solvent, avoided the pollution of product, make the product that obtains meet food sanitation standard fully as extraction and various processing;
(4) all recyclable and recycle of the ethanol that uses in each production process has reduced production cost;
(5) this production method does not have the soda acid discharging, meets environmental protection requirement.
Description of drawings
Fig. 1 is the process flow sheet of the inventive method.
Embodiment
Further specify method of the present invention with embodiment below, be understandable that, the specific embodiment of the present invention can not constitute any restriction to protection domain of the present invention.Embodiment 1 alcohol deposition method separation and purification soybean isoflavones, soybean saponin, soybean oligosaccharide and functional soybean protein concentrate
(1) takes by weighing low temperature desolventizing dregs of beans 400 grams, be divided in four 1000ml tool plug triangular flasks, add 70% ethanolic soln 600ml respectively, be placed on after shaking all in 60 ± 2 ℃ the water-bath, shake single-trip container every 10min, take out triangular flask after three hours, filter, the dregs of beans after obtaining filtered liquid and extracting with 200 mesh sieves;
(2) repeat above-mentioned steps, merge the filtered liquid of each time, this is called extraction liquid A; Dregs of beans after obtaining simultaneously extracting;
(3) the extraction liquid A small-sized ultra-filtration equipment that U.S. pall company produces, zirconium white mineral membrane element, the aperture is 20nm, and film pressure is set at 20psi (0.14Mpa), and flow velocity is set at 7-7.5ml.min
-1, obtain trapped fluid C and see through liquid B.Being concentrated into pol through liquid B with rotary negative pressure evaporation device is 35 °, obtains concentrated solution D 170ml, reclaims ethanol simultaneously;
(4) in concentrated solution D, add 95% ethanol 1020ml, stir 30min at normal temperature (20-25 ℃), static maturation 3 hours, natural separation obtains upper strata liquid E and beds of precipitation F 86 grams behind the alcohol precipitation.Upper strata liquid E reclaims ethanol with rotary negative pressure evaporation device, obtains concentrated solution G, is 151ml;
(5) in concentrated solution G, add 755ml distilled water, stir (30rpm) 20min, separate (3000rpm) 15min with the LD5-10 whizzer and obtain throw out H and supernatant liquor I with agitator.It is 93.5% soybean isoflavones that throw out H obtained 0.52 gram content in 1.5 hours through vacuum-drying (0.096Mpa);
(6) beds of precipitation F of alcohol precipitation step (4) is with 516ml distilled water wash, filtration, after the processing of walnut activated carbon decolorizing, with the concentrated oligose syrup 68ml (total pol is 85%) that obtains of rotary negative pressure evaporation device;
(7) supernatant liquor I crosses AB-8 (Chemical Plant of Nankai Univ.) macroporous resin column, cross post 45min, carry out fractionation by adsorption, to wash pol be zero degree with distillation again, carries out desorb with 50% ethanolic soln then, obtains stripping liquid 215ml, reclaim ethanol with rotary negative pressure evaporation device, obtain concentrated solution J, concentrated solution J was obtained soybean saponin 1.76 grams in 1.5 hours with negative-pressure vacuum drying (0.096Mpa), content is 70%;
(8) after the dregs of beans drying after the extraction was handled, pulverizing, screening obtained 305 gram soybean protein concentrates [protein (butt): 72.1.5%, NSI 5.6%];
(9) soybean protein concentrate 50 grams that previous step made suddenly add in the 1000ml beaker, press solid-liquid ratio and added distilled water in 1: 15, place water-bath, add papoid with enzyme-to-substrate than 3500 units/gram, bath temperature is transferred to 60 ℃, is stirred 30rpm, hydrolysis 3 hours, the steaming 5min that boils, concentrate 1.5 hours with the negative-pressure vacuum drying installation, 60 ± 2 ℃ of temperature, vacuum tightness transfer to 0.096Mpa,, take out the back naturally cooling, obtain 48 gram functional soybean protein concentrates (NSI 81.5%) with pulverizer pulverizing, screening.
The suitability for industrialized production example of embodiment 2 alcohol deposition method separation and purification soybean isoflavones, soybean saponin, soybean oligosaccharide and functional soybean protein concentrate
(1) from opening for feed, puts into the low temperature desolventizing soybean meal, adopt counter current continuous extraction equipment and Vltrasonic device to extract, regulating input speed is 300Kg/ hour, and solvent flow rate is 800Kg/ hour, extraction temperature is 60 ℃, and extraction liquid is transported to filter bank through the transfusion impeller pump.
(2) extraction liquid obtains extraction liquid A through coarse filtration, the smart filtration by being pumped in the hold tank.
(3) holding back Membralox aperture that 10t extraction liquid A produces with U.S. pall company is that micro-filtration and the Membralox aperture of 5.0 μ m (2-aluminum oxide) is 20nm (zirconium white) ultra-filtration equipment, carry out micro-filtration and uf processing, pressure is that 0.14Mpa, flow velocity are set at 100Kg.min
-1, obtain seeing through liquid B and trapped fluid C.See through liquid B and reclaim ethanol with falling film evaporator and concentrate simultaneously, temperature is that 60 ℃, pressure are 0.095Mpa, and obtaining concentrated solution D is 0.76 ton.
(4) concentrated solution D in Alcohol-settling tank, slowly adds 95% ethanol 3.8t with pump delivery, stirs 30min, static maturation 3 hours down at normal temperature (20-25 ℃), and natural separation obtains upper strata liquid E and beds of precipitation F 0.39t behind the alcohol precipitation.Upper strata liquid E reclaims ethanol with the falling film type evaporation device, and obtaining concentrated solution G is 0.65t;
(5) in concentrated solution G, add the 3.25t reverse osmosis water, stir well that (30rpm 20min), obtains throw out H and supernatant liquor I with the separation of GQ142G type tubular-bowl centrifuge.Throw out H packs into and is sent in the vacuum drying oven in the drying tray, and pressure is 0.096Mpa, and temperature is 60 ℃, pulverize with pulverizer after dry 1.5 hours, and bolting, obtaining 2.16kg content is 91.2% soybean isoflavones;
(6) beds of precipitation F of alcohol precipitation step (4), with the washing of 2.1t reverse osmosis water, filter, slowly by the activated carbon decolorizing post decolour handle after, obtain oligose syrup 145kg (always pol is 80%) with the concentration tank evaporation concentration;
(7) supernatant liquor I crosses AB-8 (Chemical Plant of Nankai Univ.) macroporous resin column, carry out fractionation by adsorption, again with reverse osmosis water wash to pol be zero degree, carry out the through post desorb with 50% ethanolic soln then, obtain stripping liquid 0.6t, reclaim ethanol with falling film type negative pressure evaporation device, obtain concentrated solution J, with concentrated solution J spraying drying, obtain soybean saponin 7.5kg, content is 70%;
(8) after the dregs of beans drying after the extraction was handled, pulverizing, screening obtained soybean protein concentrate [protein (butt) content 70.2%, NSI 5.6%];
(9) get 1 ton of the soybean protein concentrate that makes in (8), press solid-liquid ratio and add reverse osmosis water at 1: 15, stir evenly, with pump delivery in flash tank, after 85 ℃ of heat treated, cool the temperature to about 65 ℃ by plate cooler, be sent in the hydrolytic decomposition pot by infusion pump, add papoid with enzyme-to-substrate than 3500U/g, temperature is transferred to 60 ℃, stir 30rpm, hydrolysis 3 hours is steamed the 5min that boils then enzyme is lost vigor, concentrate in vacuum concentration pot with pump delivery, 60 ± 2 ℃ of temperature, vacuum tightness transfers to 0.096Mpa, uses the spray-drier drying then, and screening obtains 965Kg functional soybean protein concentrate (NSI 82.3%).
Described embodiment of the present invention now in detail, clearly can do a lot of improvement and variation for a person skilled in the art and can not deviate from essence spirit of the present invention.All these changes and improvements are all within protection scope of the present invention.
Claims (10)
1, the method for preparing soybean isoflavones, soybean saponin, oligose and soybean protein concentrate, this method may further comprise the steps:
(1) defatted soybean meal or the soybean embryo ethanolic soln with 40-80% is extracted, obtain the dregs of beans after extraction liquid and the extraction after the filtration;
(2) extraction liquid separates with ultra-filtration membrane after filtration with microfiltration membrane, obtains trapped fluid and sees through liquid;
(3) (1: 4-6) alcohol of adding high density carries out the alcohol precipitation processing to the concentrated solution that obtains after evaporation concentration through liquid, obtains the upper strata liquid and the beds of precipitation, and wherein said alcohol is lower primary alcohols by volume;
(4) alcohol precipitation is handled the beds of precipitation obtain and is washed with water, and the upper strata liquid that centrifugation obtains obtains the soybean oligosaccharide syrup in decolouring, after concentrating, and further processing can obtain the soybean oligosaccharide powder;
(5) alcohol precipitation is handled the upper strata liquid obtain and is removed through evaporation and add the water centrifugation after desolvating, and obtains supernatant liquor and throw out, and described throw out obtains high-purity soybean isoflavone after treatment;
(6) supernatant liquor that obtains of above-mentioned steps carries out fractionation by adsorption with Semi-polarity or polar macroporous resin, obtains soybean saponin;
(7) dregs of beans after the extraction that obtains of the first step extraction is through washing with alcohol, and evaporation obtains soybean protein concentrate (nitrogen soluble index NSI is about 5-10%) after removing and desolvating; Described soybean protein concentrate is added water be modulated into the soybean protein breast, handle, obtain functional soybean protein concentrate, its nitrogen soluble index NSI 〉=80% through heating with neutral proteolytic enzyme.
2, the method for preparing soybean isoflavones, soybean saponin, oligose and soybean protein concentrate according to claim 1, extraction step wherein may be carried out batchwise, and also can carry out continuously.
When the method that 3, prepares soybean isoflavones, soybean saponin, oligose and soybean protein concentrate according to claim 2, wherein said extraction step are carried out continuously, can adopt counter current continuous extraction equipment, carry out with the counter-current extraction mode.
4, the method for preparing soybean isoflavones, soybean saponin, oligose and soybean protein concentrate according to claim 2, the extractor serial or parallel connection can be connected when wherein the extraction step intermittent type carries out, and can adopt counter-current extraction, or the mode that combines with forward extraction of adverse current, to reach the effect of quick mass transfer.
5, the method for preparing soybean isoflavones, soybean saponin, oligose and soybean protein concentrate according to claim 1, extraction step is wherein carried out 40-65 ℃ temperature range.
6, the method for preparing soybean isoflavones, soybean saponin, oligose and soybean protein concentrate according to claim 1, wherein the resulting trapped fluid of step (2) obtains soybean protein milk sap after reclaiming ethanol, the synthetic soybean protein concentrate breast of institute in this soybean protein milk sap and step (7) the preparation functional soybean protein concentrate technology is merged, be used to prepare functional soybean protein concentrate.
7, the method for preparing soybean isoflavones, soybean saponin, oligose and soybean protein concentrate according to claim 1, the lower primary alcohols preferred alcohol that wherein said alcohol precipitation step adopts.
8, the method for preparing soybean isoflavones, soybean saponin, oligose and soybean protein concentrate according to claim 7, wherein said ethanol are 95% ethanol.
9, the method for preparing soybean isoflavones, soybean saponin, oligose and soybean protein concentrate according to claim 1, the high density alcohol in the wherein said alcohol precipitation step be meant add behind this alcohol in the system should alcohol concentration reach more than 80%.
10, the method for preparing soybean isoflavones, soybean saponin, oligose and soybean protein concentrate according to claim 1, the method of enzymatically treating of wherein said soybean protein concentrate is to add neutral protease by enzyme-to-substrate than 1500-5000U/g, and reacts at temperature 45-65 ℃.
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| CN111944010B (en) * | 2020-08-20 | 2023-05-09 | 运城学院 | Extraction equipment and method for soybean protein, isoflavone and oligosaccharide in bean product wastewater |
| CN114306421A (en) * | 2021-06-18 | 2022-04-12 | 山东东宇生态科技有限公司 | Method for preparing isoflavone and saponin in soybean |
| CN117362084A (en) * | 2023-10-27 | 2024-01-09 | 中山市承铭农业技术开发有限公司 | Amino acid water-soluble fertilizer prepared from soybeans and production process |
| CN117362084B (en) * | 2023-10-27 | 2024-07-05 | 中山市承铭农业技术开发有限公司 | Amino acid water-soluble fertilizer prepared from soybeans and production process |
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