CN1682593A - Bacillus thuringiesis particle agent and its preparing method and use - Google Patents
Bacillus thuringiesis particle agent and its preparing method and use Download PDFInfo
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- CN1682593A CN1682593A CN 200410031122 CN200410031122A CN1682593A CN 1682593 A CN1682593 A CN 1682593A CN 200410031122 CN200410031122 CN 200410031122 CN 200410031122 A CN200410031122 A CN 200410031122A CN 1682593 A CN1682593 A CN 1682593A
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Abstract
The granular Bacillus thuringiesis preparation has toxicity valence of 200-16000 IU/mg and live spore number of 0.5-40 billion/g. It is prepared with Bacillus thuringiesis spawn in 1-10 wt% and supplementary material in 10-99 wt% by mixing, sterilizing, fermentation, and drying. It is used in preventing and killing lepidopterous pests and ground pests, and the applied quality is 0.375-75 Kg with live spore number of 0.5-40 billion/g or toxicity valence of 200-16000 IU/mg each ha. The present invention has the features of low cost, no toxicity, no residue, long pesticidal effect, etc.
Description
[technical field]
The present invention relates to bacillus thuringiensis,Bt (hereinafter to be referred as Bt) preparation and preparation method thereof, particularly, the present invention relates to Bt granula and preparation method thereof.
[background technology]
In the prior art, there is not pure Bt granula product.When the corn borer of Bt control of maize trumpet period or soil insect, be to apply liquid fermentation compounding patent medicine sand or medicine soil after concentrating gained Bt suspending agent, Bt suspension emulsion or Bt wetting powder etc. and mixings such as a certain proportion of fine sand or fine earth in the corn horn mouth or spread fertilizer over the fields in soil.Its weak point is: 1, be difficult for mixing during user's compounding, and time-consuming; 2, effectively insecticidal constituent easily causes artificial waste with wind drift etc. when being mixed; 3, effective insecticidal constituent is adsorbed in the surface of fine sand or fine earth, easily sees the light passivation.The Bt mixing particle agent, it is to be that the Bt microbial inoculum of carrier and 3% carbofuran Granules mix with 20-40 order river sand that CN 1043603A Chinese patent has disclosed this mixture, its weak point is that the furans pellet is the chemical pesticide of high malicious high residue, the row of the agricultural chemicals of forbidding when being listed in nuisanceless or pollution-free food production by " PIC " signatories to a treaty (comprising China), thereby this Bt mixing particle agent can not use in pollution-free food production.
[summary of the invention]
The technical issues that need to address of the present invention just are to provide a kind of Bt granula and its production and use.
For addressing the above problem, technical scheme provided by the present invention is:
Bacillus thuringiensis,Bt of the present invention (Bt) preparation is a granule, and its toxicity evaluation is 200-16000IU/mg, and Bt granula living spores number is 5-400 hundred million/g.
The preparation method of bacillus thuringiensis,Bt of the present invention (Bt) granula is that the employing percentage by weight is the Bt bacterial classification of 1-10%, and adding percentage by weight is the auxiliary material of 10-99%, mixes sterilization, fermentation, and drying forms.
Bt bacterial classification of the present invention can be the solid fermentation material.
Preferably, Bt bacterial classification of the present invention is Bt bacterium powder or Bt bacterium liquid.
Auxiliary material of the present invention is wheat bran, rice bran, cavings, straw crushed material, corn flour, cake powder, lime.
Fermentation condition of the present invention is that temperature is 20-38 ℃, and relative moisture is 50-100%, and the time is 24-72 hour, and fermentation mode is fermentation of solid open type or the closed fermentation of solid.
Preferably, solid open type fermentation of the present invention is preserved moisture for solid thin layer and is fermented or shallow tray fermentation, and the closed fermentation of described solid is an air pressure oscillation fermenter.
Drying of the present invention comprises natural air drying, oven dry or pneumatic conveying drying, and baking temperature is 40-70 ℃.
The Bt granula moisture that the present invention makes is 0-20%, and the living spores number is at 5-400 hundred million/g, and toxicity evaluation is at 200-2000IU/mg, and particle diameter is the 1-100 order.
The purposes of bacillus thruringiensis granule of the present invention is that it is used to prevent and treat lepidoptera pest and soil insect, and it is that 5-400 hundred million/g or toxicity evaluation are the Bt granula 0.375-75kg of 200-16000IU/mg that per hectare is used the living spores number.
Lepidoptera pest of the present invention is corn borer, cotton bollworm, oriental tobacco budworm, beet armyworm, prodenia litura, and described soil insect is wireworm, grub, mole cricket, cutworm and ground maggot.
During use, the corn trumpet period with the Bt granula artificial or mechanical means lose in the horn mouth of executing at corn, be in the lobus cardiacus of corn, lepidoptera pests such as control of maize snout moth's larva, cotton bollworm, it is that 5-400 hundred million/g or toxicity evaluation are the Bt granula 0.375-75kg of 200-16000IU/mg that per hectare is used the living spores number.
Perhaps, when whole ground, evenly be spread on the soil table after his granula of Bt granula or B and inorganic fertilizer, bio-fertilizer, fertilizer mixed thoroughly or apply in the soil layer, soil insects such as control wireworm, grub, mole cricket, cutworm and ground maggot, per hectare counts 5-400 hundred million/g with living spores or toxicity evaluation is the Bt granula 0.375-75kg of 200-16000IU/mg.
Above-mentioned inorganic fertilizer comprises urea, MAP, diammonium phosphate, potassium dihydrogen phosphate, various Nitrogen, Phosphorus and Potassium, compound fertilizer, bio-fertilizer comprises the single bacterial manure of biological N..P.K, biological N..P.K composite bacterial fertilizer, and fertilizer comprises compoiste fertilizer, mixed organic manure.
When using, can be according to actual conditions medications such as controlling object, content, insect pest situation generation weight and bacterial classifications.With the Bt granula of a kind of bacterial classification and content, insect pest situation takes place use high magnitude when heavy, and insect pest situation takes place when light with hanging down value; Different content Bt granula, when using high-load, consumption will lack, and when using low content, it is high that consumption is wanted.When corn trumpet period control of maize snout moth's larva, as being the Bt granula of 2000IU/mg with 10,000,000,000/gram bacterial strain, toxicity evaluation, using dosage is the 3-6kg/ hectare; And be the 15-30kg/ hectare with the same bacterial classification Bt granula usage amount of toxicity evaluation 800IU/mg.When controlling underground pest, as the Bt granula with 10,000,000,000/gram bacterial strain, toxicity evaluation 4000IU/mg, using dosage is the 6-12kg/ hectare.
Controlling object and using dosage are listed as follows:
| Controlling object | Prevent and treat optimum period | Using dosage (gram/mu) | The dispenser position | Interval (my god) | Application process |
| Diamond-back moth, Oeobia undalis | Before the third-instar larvae Sheng phase | 100-150 | Blade back, cabbage heart | 7-10 | Spray |
| Beet armyworm, prodenia litura | Lay eggs the Sheng phase just | 200-400 | Blade back, cabbage heart | 4-5 | |
| The beautiful snout moth's larva of melon, cabbage caterpillar, rice plant skipper, greenish brown hawk moth, oriental tobacco budworm | Larva peak period | 100-200 | Blade back, tender leaf feeding site | 10-14 |
| The wild snout moth's larva of beans, lima bean pod borer | Lay eggs the Sheng phase just | 200-300 | Bud, young pod | 4-5 | Mist |
| Cotton bollworm, rice leaf roller | Lay eggs the Sheng phase just | 200-300 | Last middle part tender leaf (point) | 4-5 | |
| Cha Chi Xiao, Zao Chi Xiao | Lay eggs the Sheng phase just | 200-300 | The harm portion of laying eggs | 4-5 | |
| Pine caterpillars, tea caterpillar | Larval phase | 600-800 | Feeding site | 10-14 | |
| Corn borer | Larval phase | 300-400 | In the horn mouth | (granule) loses the heart | |
Advantage of the present invention is:
1, cost is low, than reproducing the low 40-50% of grain system Bt granula behind the liquid fermentation;
2, the residue that the Bt solid fermentation is carried after dense is turned waste into wealth
3, substitute lepidoptera pest and soil insects such as high malicious high residue chemical pesticide carbofuran Granules control of maize snout moth's larva, cotton bollworm with biopesticide Bt granula nontoxic, noresidue;
4, effective insecticidal constituent of Bt granula (gemma, crystal, exotoxin) has slow releasing function, and the desinsection longevity of residure is long;
5, easy to use, simple, save labor, do not need to add fine sand or fine earth, directly just can lose and execute or spread fertilizer over the fields.
The natural fragrant and sweet flavor that 6, can keep corn is not damaged because of the dispenser desinsection makes the mouthfeel of corn (particularly sweet-waxy maizes).
[embodiment]
Embodiment 1
After 400 wheat bran that restrain, 1-10 gram lime, 430 gram water mixing sterilizations, inoculum density is the abundant No. 1 Bt solid fermentation material of 1: 50 Wan Nongda 830 grams in temperature is that 20 ℃, relative moisture are under 98% the condition, through Bt solid open type fermentation 28 hours, when reaching 30%, gemma and the crystal rate of taking off stop fermentation and discharging in time, dry in the heated kang drying room about 55 ℃, making moisture is≤10%, and the living spores number is in the Bt of 50-400 hundred million/g granula.
Embodiment 2
Cavings, bran mixture with 500 grams, after the lime of 1-10 gram, the water of 500 grams mix sterilization, inoculum density is former powder 1000 grams of abundant No. 1 Bt of 1: 45 Wan Nongda, in temperature is that 38 ℃, relative moisture are under 50% the condition, through Bt solid open type fermentation 24 hours, when reaching 30%, gemma and the crystal rate of taking off stop fermentation and timely discharging, dry in the heated kang drying room about 40 ℃, making moisture is 10%, and the living spores number is in the Bt of 50-400 hundred million/g granula.
Embodiment 3
After the lime of the corn flour with 400,1-10 gram, the water of 500 grams mix sterilization, inoculum density is the abundant No. 1 Bt wetting powder of 1: 50 Wan Nongda 1000 grams in temperature is that 26 ℃, relative moisture are under 70% the condition, through Bt solid open type fermentation 48 hours, when reaching 30%, gemma and the crystal rate of taking off stop fermentation and discharging in time, dry in the heated kang drying room about 70 ℃, making moisture is 5%, and the living spores number is in the Bt of 50-400 hundred million/g granula.
Embodiment 4
After the lime of the soybean-cake flour with 500,1-10 gram, the water of 500 grams mix sterilization, inoculum density is abundant No. 1 Bt suspending agent 1000 grams of 1: 45 Wan Nongda, in temperature is that 26 ℃, relative moisture are under 70% the condition, through Bt solid open type fermentation 72 hours, when reaching 30%, gemma and the crystal rate of taking off stop fermentation and discharging in time, dry in the heated kang drying room about 55 ℃, making moisture is 5%, and the living spores number is in the Bt of 50-400 hundred million/g granula.
Embodiment 5
Select area 35m in the Linquan County, Anhui Province
2, control of maize snout moth's larva on the 187 strain summer corns, with the Bt granula 10.5g of embodiment 1 preparation, wherein 184 strains manually apply the Bt granula in the corn lobus cardiacus at the corn trumpet period, and the residual effect toxicity evaluation was 400IU/mg when the control efficiency after 5 days was 89.7%, 20 day.With the same toxicity evaluation of bacterial strain, mix summer that fine sand prevents and treats same area, homophyletic number during with full trumpet period corn borer with the Bt wetting powder of dosage, its control efficiency after 5 days is that the residual effect toxicity evaluation after 79.2%, 20 day is 0IU/mg.
Embodiment 6
Select area 333.35m in the Linquan County, Anhui Province
2, autumn planting wheatland control soil insect.After mixing thoroughly with 25kg three elements inorganic composite fertilizer with the Bt granula 250g of embodiment 2 preparation, spread fertilizer over the fields in the face of land, cultivated bonnet is under soil.Wheat cultivation was emerged in back 30 day time, all observed the wheat seeding situation that causes harm every day, was as the criterion with dead seedling, and the dead seedling number of its Bt granula is 100 strains/30 day.With the same place of production, same bacterial classification, same toxicity evaluation, same dosage, mix 25kg with plot Bt wetting powder and spread fertilizer over the fields in the face of land with the three elements inorganic composite fertilizer in the place of production, plough bonnet under soil, behind the insemination and emergence in 30 day time, observe the wheat seeding death condition every day, dead seedling number is 450 strains/30 day, and is that the dead seedling number of rand of uptake is some more when spreading fertilizer over the fields.
Claims (10)
1, a kind of bacillus thuringiensis,Bt (Bt) preparation is characterized in that it is a granule, and its toxicity evaluation is 200-16000IU/mg, and Bt granula living spores number is 5-400 hundred million/g.
2, a kind of preparation method of bacillus thruringiensis granule is characterized in that it is that the employing percentage by weight is the Bt bacterial classification of 1-10%, and adding percentage by weight is the auxiliary material of 10-99%, mixes sterilization, fermentation, and drying forms.
3, preparation method according to claim 2 is characterized in that described Bt bacterial classification is a Bt solid fermentation material.
4, preparation method according to claim 2 is characterized in that described Bt bacterial classification is Bt bacterium powder or Bt bacterium liquid.
5, preparation method according to claim 2 is characterized in that described auxiliary material is wheat bran, rice bran, cavings, straw crushed material, corn flour, cake powder, lime.
6, preparation method according to claim 2 is characterized in that described fermentation condition is that temperature is 20-38 ℃, and relative moisture is 50-100%, and the time is 24-72 hour, and fermentation mode is fermentation of solid open type or the closed fermentation of solid.
7, the preparation method who states according to claim 6, it is characterized in that the fermentation of described solid open type is preserved moisture for solid thin layer ferments or shallow tray fermentation, and the closed fermentation of described solid is an air pressure oscillation fermenter.
8, preparation method according to claim 2 is characterized in that described drying comprises natural air drying, oven dry or pneumatic conveying drying, and baking temperature is 40-70 ℃.
9, the purposes of bacillus thruringiensis granule according to claim 1, it is characterized in that: it is used to prevent and treat lepidoptera pest and soil insect, and it is that 5-400 hundred million/g or toxicity evaluation are the Bt granula 0.375-75kg of 200-16000IU/mg that per hectare is used the living spores number.
10, purposes according to claim 9 is characterized in that described lepidoptera pest is corn borer, cotton bollworm, oriental tobacco budworm, beet armyworm, prodenia litura, and described soil insect is wireworm, grub, mole cricket, cutworm and ground maggot.
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| CN 200410031122 CN1682593A (en) | 2004-04-12 | 2004-04-12 | Bacillus thuringiesis particle agent and its preparing method and use |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109511680A (en) * | 2018-11-27 | 2019-03-26 | 何美英 | A kind of biological pesticide and preparation method thereof of the fatty glyceride of Residual oil containing rice residue |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109511680A (en) * | 2018-11-27 | 2019-03-26 | 何美英 | A kind of biological pesticide and preparation method thereof of the fatty glyceride of Residual oil containing rice residue |
| CN109511680B (en) * | 2018-11-27 | 2021-12-07 | 上海沪联生物药业（夏邑）股份有限公司 | Biopesticide containing rice residue oil and fatty acid glyceride and preparation method thereof |
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