CN1681385A - Protein transport enhancer for transgenic plants - Google Patents
Protein transport enhancer for transgenic plants Download PDFInfo
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- CN1681385A CN1681385A CNA038219751A CN03821975A CN1681385A CN 1681385 A CN1681385 A CN 1681385A CN A038219751 A CNA038219751 A CN A038219751A CN 03821975 A CN03821975 A CN 03821975A CN 1681385 A CN1681385 A CN 1681385A
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- Prior art keywords
- protein
- plant
- transport enhancer
- protein transport
- genetically modified
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/16—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds containing nitrogen-to-oxygen bonds
- A01N33/18—Nitro compounds
- A01N33/20—Nitro compounds containing oxygen or sulfur attached to the carbon skeleton containing the nitro group
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
Expression and stability of desirable proteins in transgenic plants are promoted and maintained by treatment with a protein transport enhancer. Preferably, the transgenic plant is a commodity crop that has been modified to express pesticidally effective protein proteins.
Description
Invention field
The present invention relates to handle genetically modified plants, especially be designed to express the method for the crops of desinsection effective protein proteins.
Technical background
Genetically modified plants have material impact to commercial agriculture, and its interests have prospect but also produced new insect control problem very much.It should be noted that to become poisonous and the selective elemination insect by the food (plant tissue) that makes target pest, reduce or eliminate the consumption of chemical insecticide thus based on crops with the plant of the genetic modification of expressing the effective crystalline protein of desinsection.
Some member who belongs to the gram-positive bacteria of bacillus produces insecticidal proteins.Understanding maximum is bacillus thuringiensis (BT), and wherein insecticidal proteins is lenticular corpusculum and the thalline that forms spore.The feature of this insecticidal crystal protein is it to effectiveness and specificity of specific insect pest (wherein a lot of insects have material impact to agricultural), with and comparatively safe to non-targeted insect kind and vertebrate (particularly people).These insecticidal crystal proteins are used for horticulture for a long time, wherein as the chemical insecticide mixture of crystal and spore of spraying, but do not obtain very ten-strike for the more wide field crop of scope.
This insecticidal proteins is made of the large protein of basic non-activity.When caterpillar had been taken in some insecticidal crystals, the alanine minimizing state of this insect midgut made this crystal separation and discharges crystalline protein.Make non-activity at this stage this albumen, but the certain enzyme in the insect gastro-intestinal Fluid is trimmed to the nuclear of its protease resistant with this albumen, this nuclear is active fully.The insecticidal proteins of activation is then in conjunction with the brush border membrane of midgut tapetum cell, and inserts cell membrane.When about 8 such insertions gathered together, they just formed hole or the passage of wearing film, and allowed cellular content to leak, and caused the death of the necessary cell of nutrient absorption.Insect loses nutrition and final hungry to death rapidly, or because the secondary bacterial infections in 24 hours and death.Dissolution of crystals, proteolysis become active parasite killing albumen and the process of special receptor combination are all made this BT albumen high degree of specificity and very favourable to environment.
Collect several thousand kinds of different bacillus thuringiensis separators and determined its insecticidal proteins composition and activity profile.A large amount of BT insecticidal proteins are now cloned and are checked order.For avoiding confusion, researcher's suggestion is used same naming system to crystal protein gene (Cry gene), according to its protein sequence and its toxicity at the insect type.
Table 1
| Gene type | Insect host | Size (kD) |
| ?CryIA | Caterpillar | 133.2 |
| ?CryIB | Caterpillar | 138.0 |
| ?CryIC | Caterpillar | 134.8 |
| ?CryIIA | Caterpillar | 70.9 |
| ?CryIIB | The fly larva | 70.8 |
| ?CryIIIA | First gram worm larva | 73.1 |
| ?CryIVA | The fly larva | 134.4 |
| ?CryIVB | The fly larva | 127.8 |
| ?CryIVC | The fly larva | 77.8 |
| ?CryIVD | The fly larva | 72.4 |
Similar and only poisonous by the protein sequence of CryI gene code to caterpillar (also being the larva of moth and butterfly, Lepidoptera); The CryII gene code is to Lepidoptera and/or diptera (fly and mosquito) is poisonous and CryIV albumen is only poisonous to diptera.The CryIII genes produce is to the poisonous albumen of beetle (coleoptera) larva.Considered the similitude and the difference of sequence between the different albumen, in above-mentioned main type, segmented.CryI group for example is divided into CryIA at first, and it is 50% same that IB and IC (although having reached CryIG now), wherein different subclass may have only on protein sequence.Also carried out finer classification and CryIA comprises CryIA (a) now, IA (b) and IA (c).
Having developed several different methods is used for genes of interest is imported crop plant cells and cultivates the plant that can educate thus.Referring to, United States Patent (USP) 6329574 Hes
Http:// www.cotton.pi.csiro.au / publicat/pest/transgen.htm, its content is all quoted as a reference at this.Institute's choosing method partial dependency targeted species, and cotton modification often relies on the natural gene transfer thing of the self-growth of its plant genetic transformation.The disease that is called Crown Gall is a kind of have soil bacteria pathogeny thing, canker that Agrobacterium causes.Early stage in the 1970's, people recognize that this bacterium causes disease by shifting himself genetic material of part in plant cell dna.The normal biochemical machine of the infected cell of gene disruption that these are parasitic also makes it make the only noval chemical compound of bacterium utilization.Further after the research, scientist can identify that those genes cause disease, and because bacterium is the simple biology that is easy to genetic manipulation, they have replaced Disease-causing gene with the gene of the potentially useful that oneself makes up.These bacteriums can be used for gene from the test tube load to plant cell then.Yet finally can both obtain this new gene behind this bacterium of not every plant contact.Remove the plant cell of not transforming with antibiotic purification.
Plant cell is to the multiple antibiotic sensitive that is used to control the bacterial infection of humans and animals.If can separate the gene that the plant cell of sening as an envoy to has resistance to certain toxicity antibiotic,, can provide and kill the not selective system of those cells of receptor gene in infection then with some genes of interest physical connection and insert Agrobacterium.For many years people have understood those and make bacterial antibiotic that the gene of resistance be arranged, and it makes the nontoxic enzyme of antibiotic have resistance by production decomposition or chemical modification.With above-mentioned gene splicing technology, the gene and having produced that the researcher can transform the enzyme of coding detoxifcation antibiotic kanamycin causes that this enzyme is produced the new heterozygous genes of this enzyme in plant cell and death when preventing that its kanamycin in lethal dose from existing.In conjunction with this antibiotic-screening system and Plant Tissue Breeding step, might gene be introduced various plants with Agrobacterium, comprise from the petunia to the cotton.
Cotton is the crop of particular importance.The transgene cotton of commercial form uses the CryIAc (BOLLGARD of Monsanto
TM) and use CryIAc and the Cry2Ab gene (BOLLGARD of Monsanto
TMII) unite the endotoxin of expressing bacillus thuringiensis.Efficient table road, land for growing field crops shows 50-70% Pest Control cotton bollworm (helicover paarmigera) and Australia cotton bollworm (H.punctigera).Also of great value is the crop plants of transforming with the Su Yun Jin Jing body virulent gene of CryET33 by name and CryET34, and described gene code is to the crystalline protein CryET33 (29-kDa) and the CryET34 (14-kDa) of coleopteran-toxic.CryET33 and CryET34 crystalline protein are poisonous to intending ostomatid (red flour beetle) larva and Japanese beetle (Japanesebeetle) larva.(referring to, United States Patent (USP) 6399330)
The application of transgenic crop has caused the new problem in the struggle of whole insect control.Some problems relate to the ripe endotoxin of expressing that reduces of plant, and it causes may producing possibility increase to endotoxic immunity the later stage season of growth (back 1/3 efficient in cotton growth season reduces) and insect.These shortcomings cause development at plantation " window ", with respect to the insect control strategy of local insect development cycle and the minimum thresholding of insect colony of having specified insecticide to use.
Physical stress of genetically modified plants and physical damage also cause endotoxin protein matter to express decline, and corresponding insect control efficiency reduces.Therefore, prolonged drought and/or high temperature can reduce that endotoxin in the genetically modified crops is expressed speed and be that protection to insect significantly descends, and needing to cause the use of insecticide spraying.
It is not fully aware of that endotoxin protein is expressed the reason that descends.In the BT cotton, theory thinks that because CMV3 5S promoter concentration reduces, gene " silence " or other are transcribed the back incident, so CryIAc gene expression descends.Also the someone thinks that it is the increase that transforms and detain because of in the plant that CryIAc albumen reduces, perhaps because growth and the old and feeble dilution that causes.People's understanding CryIAc transcriptional level in immature and ripe BT vegetable lamb is all unstable.
Be used to handle the genetically modified plants of expressing the effective albumen of desinsection if exist, and promote still that when plant maturation, physical stress and physical damage the system of these protein expressions will be very favorable.
The invention summary
The purpose of this invention is to provide the method for handling genetically modified plants, the especially genetically modified crops of expressing insecticidal proteins, and be used in particular for expressing the genetically modified crops of insecticidal protein.
Another object of the present invention provides and prolongs the time method that expressed proteins exists with q.s, and it is the harmful insect of food that described amount is enough to control with processed plant.
According to these and other objects, can find out from this paper, the method of treatment in accordance with the present invention transgenic crop comprises uses protein transport enhancer to the leaf of genetically modified plants, described leaf is expressed the desinsection effective protein proteins, and described reinforcing agent promotes the expression and/or the stability of the effective albumen of desinsection in the processed plant.
Although do not wish to accept by restriction, the inventor thinks that this protein transport enhancer is with following one or more mode effect: (a) form of the protectiveness water substitute of crop cell film when lack of water pressure is arranged; (b) as the protein stabilizing agent of purpose insecticidal proteins; And/or (c) as bond in conjunction with the albumen of the motion of transhipment machine in the promotion process plant.As a result, although the physical stress and the plant injury of lack of water are arranged, the genetically modified crops of handling according to the present invention are expressed insecticidal proteins and it are transported to fruit tissue.The inventor think treatment in accordance with the present invention also will be in plant growing and maturation the insecticidal proteins of continuous expression level of significance.
Detailed Description Of The Invention
Handle genetically modified plants according to the present invention with protein transport enhancer; although described reinforcing agent fully stimulates and/or protects feasible physical damnification, growth and the plant maturation that has lack of water, plant tissue of transhipment machine in cellular expression and the plant, keep the insecticidal proteins of desired level in plant tissue, fruit and the seed.In the genetically modified plants tissue, keep the level of efficiency that destination protein expression and concentration help to keep insect control preferably, further reduce the consumption of the needed insecticide of antagonism decrease in efficiency at present, and will help to prevent the insect survival that contacts and prevent that the resistant insect population from producing.
Must know that percentage used herein is the weight ratio with respect to gross weight, except as otherwise noted.
Be suitable for protein transport enhancer of the present invention and comprise one or more compound and the acceptable salt of agrochemistry according to formula 1 structure:
Formula 1
Wherein:
X is NO
2
Y is H, C
1-C
6Alkyl, C
1-C
6Alkoxyl, C
2-C
6Thiazolinyl, and
Z is C or N.
Specifically, Y part can be methyl, ethyl, propyl group, butyl, different-butyl, amyl group, hexyl, methoxyl group, ethyoxyl, propoxyl group, butoxy, different-butoxy, amoxy and own oxygen base.
The used preferred protein transport enhancer of the present invention comprises that according to one or more compound of following formula structure and the salt of compound, wherein X is the nitro with respect to hydroxyl ortho position or contraposition, and Y is hydrogen or C
1-C
3Alkoxyl, and Z is a carbon atom.The salt that is fit to comprises water-soluble alkali metal salts (especially sodium salt and sylvite), ammonium salt and other non-plant toxicity or the water soluble salt favourable to environment.
Most preferred protein transport enhancer comprises following associating: the sodium salt of p-nitrophenol (A), o-nitrophenol (B) and 2-methoxyl group-5-nitrophenol (C).Especially preferred, protein transport enhancer comprises that these salt are (0.1-10) at A: B: C: (0.1-10): the mixture of 1 proportion.These salt can be from ATONIK Asahi Chemical Mfg.Co., and Ltd is to buy at 2: 3: 1 with A: B: C.
Protein transport enhancer according to the present invention is used with the ratio (gAI/ac) of general every acre of processed field less than every kind of active component of 20 grams.Preferably, these reinforcing agents use with the ratio of 1-20gAI/ac scope and more preferably use with the ratio of 3-18gAI/ac scope.Especially preferred, based on gross weight, use protein transport enhancer salt, and use with the ratio of every acre of 0.5-20 fluid ounce (oz/ac) scope with the scope of 0.01-5 weight %.
According to protein transport enhancer of the present invention can with other material use in conjunction of the agricultural use of one or more active component, spray aids, liniment or suitable target plant.The active component example that can follow protein transport enhancer according to the present invention to unite use comprises weed killer herbicide, plant growing reinforcing agent, plant growth retardant, phyllody fertilizer, fungicide (external application and systematic) and insecticide (external application and systematic).
Available weed killer herbicide comprises triazine (for example, atrazine), urea, glyphosate, sulphosate (sulfosate), glyfosinate and sethoxydim (sethoxydim).
Be suitable for plant growing reinforcing agent of the present invention and comprise auxin, at least a as in the gibberellin of identifying in 84 (gibberillin), and preferred GA
3, GA
4, GA
5, GA
7, and GA
9, cell factor (for example, zeatin, kinetin, benzyladenine, dihydro zeatin and isopentenyl gland purine); Growth hormone (for example, heteroauxin (IAA), indolebutyric acid (IBA) and methyl (NAA)); With 2,4, the multi-hydroxy carboxy acid of 5 and 6 carbon structures; Ethephon and change crust.
Be suitable for plant growth retardant of the present invention and comprise chlorine chlormequat, first piperazine (mepiquat chloride) and maleic acid hydrazides and salt thereof.This plant growth regulating thing influence and change vegetable metabolic process and strengthen or hinder plant growing.All these materials can use according to application rate and time that its manufacturer provides in the description of product.
Systemic fungicide of the present invention be will have benefited from and tridemorph, metalaxyl, iprodione, phosethyl-Al, topsin, benomyl, triazolone, carboxin, oxycarboxin, carbendazim, thiabendazole, Milstem, bupirimate and Milcurb comprised.
The systemic insecticides that is fit to comprises Aldicarb, orthen, furadan, Rogor, thimet and Terbufos.
Make and express and the affected specific mechanisms of transporting mechanism and unclear.For example, test this handled thing of wheat spike of not transformation to the albumen that is transported in the wheat plant nucleus of the seed with radiolabeled nitrophenolate.The hydrating agents effect of phenol material that studies show that in addition as cell membrane.
In cotton, people recognize that cotton seeds needs a large amount of albumen, and seed produces velveteen from carbohydrate in botanical system.Show the increase of seed and function with ATONIK test normal plants (promptly not genetic modification or change) to express insecticidal proteins, the normal part of crop plants metabolic function.This effect is consistent with the effect of plant growth regulating thing, but anything of the relevant effect of handling plant of hint not necessarily, and described plant is by genetic modification and the part of the albumen crop normal function of production appointment.
The genetically modified plants that can handle according to the present invention generally are those plants that contained the gene of expressing required desinsection effective protein proteins by the wild type transformation.Preferably, genetically modified plants comprise the gene of expressing the desinsection effective protein proteins, and described albumen provides resistance to the attack and the infection of insect, bacterium, fungi, mould, acarid etc.
Two kinds of special efficient genes are CryIAc gene (by the CMV35S promoters driven) and Cry2Ab gene.These genes can separately or be united insertion cotton, corn, wheat, Chinese sorghum, soybean and similar daily crops so that the protection to multiple harmful insect to be provided.Valuable especially is to transform and the cotton dried (for example BT cotton) of expression desinsection effective protein proteins.The seed that stays of the BT cotton that can handle according to the present invention can trade mark BOLLGARD
Or BOLLGARD
II (MonsantoCo.) buys.
Protein transport enhancer according to the present invention is applied to genetically modified plants with certain ratio and time in plant growing cycle or season, make that keeping insecticidal proteins in plant tissue expresses and stabilisation.Following table has been identified preferred genetically modified plants to be processed and generally useful, preferably and the amount of the protein transport enhancer of very preferred application rate.
Table 2
| Genetically modified plants | Application rate (Oz/Ac) | Preferred application rate (Oz/Ac) | Preferred application rate (Oz/Ac) |
| The BT cotton | 1-100 | ?1-50 | ?5-30 |
| The BT cotton | 1-100 | ?1-50 | ?5-30 |
Embodiment
Vegetable lamb in transgenosis Bt cotton in 2 liters the basin (Deltapine Nucotn B) and not transformation is sprayed with ATONIK under controlled condition, this is a kind of product of being commonly considered as growth regulator that contains, but this growth regulator to the daily crops of genetic modification as the protein transport enhancer function well.ATONIK contains the sodium salt of p-nitrophenol (0.3%), o-nitrophenol (0.2%) and 5-nitrophenol (0.1%).(TL) handles cotton at the 7th true leaf, and the trunk leaf that stretch out on sampling top after 10 days.Maintain the temperature at 76-86 degrees Fahrenheit and sufficient moisture.Pressure condition with ATONIK spraying, and is accepted: the water supply (drying condition) of rising temperature (86-102 degrees Fahrenheit) and the deficiency trunk leaf that stretch out on (7TL+15 days) top of taking a sample in sampling back (7TL+10 days) after 5 days.After this 5 days (7TL+20 days) are in the same terms, to plant sampling leaf and squares.
After each sampling, be placed on tissue sample in the sealed bag and take immediately and carry out corn earworm and feed on the equipment of test.The spray applications sample begins to feed to the first time the 24th, 48,72 and 96 hours and to the second time spray applications sample measured the corn earworm lethality on the the 72nd and 96 hour.
After finishing lethality research, will take away from the blade section of representative cotton plants and carry out analysis of protein.Store at the place of its 2nd, 6,8 and 10 joints collection blade and in-80 degrees Fahrenheits from the top.
The corn earworm lethality of the blade of collecting in the sample of first collection is shown in table 3
Table 3
| Handle for the first time | Corn earworm lethality (at 7TL+10 days) | |||
| 24 hours | 48 hours | 72 hours | 96 hours | |
| Contrast (Bt cotton) | ?0 | ?28.3 | ?56.7 | ?58.3 |
| ?ATONIK?5oz/ac | ?0 | ?35.0 | ?60.0 | ?68.3 |
| ?ATONIK?10oz/ac | ?1.7 | ?43.3 | ?61.7 | ?71.7 |
| ?ATONIK?20oz/ac | ?3.3 | ?60.0 | ?78.3 | ?81.7 |
| Contrast (non-Bt cotton) | ?0 | ?1.7 | ?1.7 | ?3.3 |
Be shown in table 4 in the corn earworm lethality of the blade of spraying back the 5th day and collection in the 10th day for the second time.
Table 4
| Handle for the second time | Corn earworm lethality (at 7TL+x days) | |||
| 15 days | 20 days | |||
| 72 hours | 96 hours | 72 hours | 96 hours | |
| Contrast (Bt cotton) | ?68.3 | ?71.1 | ?90.0 | ?95.0 |
| ?ATONIK?5oz/ac | ?63.3 | ?71.1 | ?81.7 | ?91.7 |
| ?ATONIK?10oz/ac | ?85.0 | ?90.0 | ?85.0 | ?96.7 |
| ?ATONIK?20oz/ac | ?83.3 | ?90.0 | ?90.0 | ?98.3 |
| Contrast (non-Bt cotton) | ?0 | ?0 | ?5.0 | ?6.7 |
The corn earworm lethality of the square of collecting 7TL+20 days (the 2nd time handle) (square) is shown in table 5.
Table 5
| Handle for the first time | Corn earworm lethality (at 7TL+20 days) | |
| 72 hours | 96 hours | |
| Contrast (Bt cotton) | ?75.0 | ?81.7 |
| ?ATONIK?5oz/ac | ?95.0 | ?98.3 |
| ?ATONIK?10oz/ac | ?88.3 | ?93.3 |
| ?ATONIK?20oz/ac | ?86.7 | ?95.0 |
| Contrast (non-Bt cotton) | ?0 | ?3.3 |
Table 6 has shown with ATONIK to be handled the height of Bt cotton and the influence of joint number amount.
Table 6
| Handle | Plant height (cm) | Joint number amount (on average) |
| Contrast (Bt cotton) | ?63.5 | ?16.7 |
| ?ATONIK?5oz/ac | ?61.7 | ?15.8 |
| ?ATONIK?10oz/ac | ?62.0 | ?16.0 |
| ?ATONIK?20oz/ac | ?62.2 | ?17.0 |
| Contrast (non-Bt cotton) | ?61.0 | ?13.8 |
The result shows that (table 4 and 5) all has higher corn earworm lethality under the pressure condition of using protein transport enhancer processing according to the present invention to cause optimum growh state (table 3) and temperature and lack of water.Yet handle with ATONIK and not cause the increase of nourishing and growing.(seeing Table 6)
Claims (8)
1. keep the method for the insecticide efficiency of genetically modified plants, these genetically modified plants comprise the gene of expressing the desinsection effective protein proteins, and described method comprises:
Handle described genetically modified plants with protein transport enhancer, this reinforcing agent is stablized the transhipment of desinsection effective protein proteins in the described plant.
2. according to the process of claim 1 wherein that described genetically modified plants are cotton or corn.
3. according to the process of claim 1 wherein that described genetically modified plants are to have transformed the cotton of expressing bacillus thuringiensis albumen.
4. according to the process of claim 1 wherein that described protein transport enhancer comprises the mixture of phenolate and guaiacolate.
5. according to the method for claim 4, wherein said protein transport enhancer is applied to described plant with the ratio of 1-100oz/ acre scope.
6. according to the method for claim 5, wherein said protein transport enhancer is applied to described plant with the ratio of 1-50oz/ acre scope.
7. according to the method for claim 6, wherein said protein transport enhancer is applied to described plant with the ratio of 5-30oz/ acre scope.
8. according to the process of claim 1 wherein that described protein transport enhancer comprises the polyalcohol that aldehyde and ketone group by reduced carbohydrate obtain.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US39971602P | 2002-08-01 | 2002-08-01 | |
| US60/399,716 | 2002-08-01 | ||
| US10/429,974 US6770598B1 (en) | 2003-05-06 | 2003-05-06 | Protein transport enhancer for transgenic plants |
| US10/429,974 | 2003-05-06 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1681385A true CN1681385A (en) | 2005-10-12 |
Family
ID=31498600
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA038219751A Pending CN1681385A (en) | 2002-08-01 | 2003-07-31 | Protein transport enhancer for transgenic plants |
Country Status (9)
| Country | Link |
|---|---|
| EP (1) | EP1538899A4 (en) |
| CN (1) | CN1681385A (en) |
| AR (1) | AR040751A1 (en) |
| AU (1) | AU2003257029B2 (en) |
| BR (1) | BR0313099A (en) |
| CO (1) | CO5690503A2 (en) |
| MX (1) | MXPA05001216A (en) |
| WO (1) | WO2004013286A2 (en) |
| ZA (1) | ZA200501290B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105409619A (en) * | 2015-11-19 | 2016-03-23 | 中国农业科学院棉花研究所 | Method for increasing content of exogenous insecticidal protein in transgenic Bt cotton |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP7029186B2 (en) | 2017-12-24 | 2022-03-03 | Ｏａｔアグリオ株式会社 | Synergistic fungicide composition containing nitrophenol compound and bactericidal active compound |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5650372A (en) * | 1995-05-30 | 1997-07-22 | Micro Flo Company | Plant treatment with bacillus strain ATCC |
| US5919999A (en) * | 1996-11-14 | 1999-07-06 | Queen's University At Kingston | Enhanced transport with a plastid membrane transport protein |
-
2003
- 2003-07-31 WO PCT/US2003/023823 patent/WO2004013286A2/en not_active Application Discontinuation
- 2003-07-31 AU AU2003257029A patent/AU2003257029B2/en not_active Ceased
- 2003-07-31 BR BRPI0313099-1A patent/BR0313099A/en not_active IP Right Cessation
- 2003-07-31 EP EP03766985A patent/EP1538899A4/en not_active Withdrawn
- 2003-07-31 AR AR20030102758A patent/AR040751A1/en unknown
- 2003-07-31 MX MXPA05001216A patent/MXPA05001216A/en active IP Right Grant
- 2003-07-31 CN CNA038219751A patent/CN1681385A/en active Pending
-
2005
- 2005-02-14 ZA ZA2005/01290A patent/ZA200501290B/en unknown
- 2005-03-01 CO CO05018883A patent/CO5690503A2/en not_active Application Discontinuation
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105409619A (en) * | 2015-11-19 | 2016-03-23 | 中国农业科学院棉花研究所 | Method for increasing content of exogenous insecticidal protein in transgenic Bt cotton |
| CN105409619B (en) * | 2015-11-19 | 2018-07-20 | 中国农业科学院棉花研究所 | A method of improving transgenic Bt cotton external source insecticidal proteins content |
Also Published As
| Publication number | Publication date |
|---|---|
| EP1538899A2 (en) | 2005-06-15 |
| AR040751A1 (en) | 2005-04-20 |
| CO5690503A2 (en) | 2006-10-31 |
| ZA200501290B (en) | 2005-11-30 |
| MXPA05001216A (en) | 2005-08-18 |
| WO2004013286A2 (en) | 2004-02-12 |
| EP1538899A4 (en) | 2006-06-28 |
| AU2003257029A1 (en) | 2004-02-23 |
| AU2003257029B2 (en) | 2008-08-28 |
| BR0313099A (en) | 2007-07-17 |
| WO2004013286A3 (en) | 2004-08-05 |
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