CN1679795A - Anti-cancer extracts from thinleaf adina root and its making method and use - Google Patents
Anti-cancer extracts from thinleaf adina root and its making method and use Download PDFInfo
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- CN1679795A CN1679795A CN 200510032665 CN200510032665A CN1679795A CN 1679795 A CN1679795 A CN 1679795A CN 200510032665 CN200510032665 CN 200510032665 CN 200510032665 A CN200510032665 A CN 200510032665A CN 1679795 A CN1679795 A CN 1679795A
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Abstract
An anticancer extract of thinleaf adina root for preparing the medicines in the form of injection, etc to treat tumors is prepared from the root of thinleaf adina through extracting in water or organic solvent, flocculating deposition, regulating acidity, purifying, recovering solvent, extracting, concentrating and drying.
Description
Technical field
The present invention relates to field of medicaments, particularly a kind of Radix Gei japonici antineoplastic extract and its production and use.
Background technology
Radix Gei japonici is the root of Rubiaceae defoliation bush plant Fructus Adinae (Adina rubella (Sieb.etZucc.) Hance), has the effect of heat-clearing and toxic substances removing, diffusing silt pain relieving.People such as Yu Rencun are " tumor is used Chinese herbal medicine always ", traditional Chinese medical science oncology, and report in 1997,72 pages, Radix Gei japonici alcohol extractum every day, L615 had inhibitory action to mouse leukemia with 5g/Kg continuous irrigation stomach or lumbar injection 6 days, and suppression ratio is 21.4%; To mouse cervical cancer cell, white mice sarcoma AK, and the rat sarcoma inhibitory action is all arranged; This prompting Radix Gei japonici alcohol extractum has the broad-spectrum anti-tumor effect.Contain catechin compounds in the flowers and fruits preface of Fructus Adinae, other has ursolic acid, oleanolic acid, cupreol, Fructus Adinae first element and alkaloid etc., but less about the effective ingredient report of Radix Gei japonici.What the people such as goes straight up in " Fructus Adinae The Chemical Constituents ", Chinese herbal medicine, and 1996,26 (6): report in 285～288, they isolate several new Quinovic acid glycoside compounds from Radix Gei japonici, but do not carry out anti-tumor activity research.Quinovic acid glucosides also is found in other anticancer plants, document Raffauf RF was once arranged, etal.Lloydia, 1978,41 (5): 432～434 mention, in ledger bark, found Quinovic acid glycoside compounds, this chemical compound has anti-tumor activity, Raffauf RF had also found this compounds in other has the vegetable drug of anti-tumor activity afterwards, and this shows that Quinovic acid glycoside compounds might also be the main anti-tumor active substance of Radix Gei japonici.
At present, the research of Radix Gei japonici is only rested on the superficial understanding of its medical material, not about the report of Radix Gei japonici anti-tumor effective component and method of separating component, preparation technique.
Summary of the invention
The objective of the invention is to overcome the shortcoming that exists in the prior art, a kind of effective site of the Chinese medicine of purification effectively Radix Gei japonici is provided, be convenient to the Radix Gei japonici antineoplastic extract preparation method of suitability for industrialized production.
Another object of the present invention is to provide a kind of Radix Gei japonici antineoplastic extract by method for preparing.
A further object of the present invention is to provide a kind of purposes of above-mentioned Radix Gei japonici antineoplastic extract.
Purpose of the present invention is achieved through the following technical solutions: the preparation method of this Radix Gei japonici antineoplastic extract comprises the steps:
(1) water or organic solvent lixiviate Radix Gei japonici medical material under 70～100 ℃ of temperature obtains leachate;
(2) add flocculating agent in leachate, the precipitation roguing obtains extracting solution;
(3) pH value with extracting solution is adjusted to 2～5, leaves standstill, and obtains supernatant;
(4) supernatant is crossed macroporous resin, use solvent elution, collect eluent;
(5) eluent is reclaimed solvent after, use ethyl acetate extraction, obtain extract;
(6), obtain the Radix Gei japonici antineoplastic extract with the extract concentrate drying.
In the step (1), described organic solvent is 30～70% (V/V) ethanol; Described Radix Gei japonici medical material can be Radix Gei japonici block or ground product; Preferred 70～80 ℃ of described extraction temperature.
In the step (1), described lixiviate number of times is 1～3 time; Each extraction time is 1～2 hour; The Radix Gei japonici of lixiviate for the first time and the weight ratio of water or organic solvent are 1: 8～10; Be 1: 4～5 with the Radix Gei japonici of lixiviate for the third time and the weight ratio of water or organic solvent for the second time.
In the step (2), leachate can be concentrated earlier, and then flocculation sediment; Described flocculating agent can be chitin, ZTC flocculating agent or gelatin, and consumption is 0.1～1% of a Radix Gei japonici medical material weight; Described precipitation can adopt filters or the centrifugalize precipitation.
In the step (3), adopt acid reagent to regulate the pH value of extracting solution, preferred hydrochloric acid, acetic acid.
In the step (4), described macroporous resin can be the macroporous resin of various nonpolar or low poles; Described solvent is an aqueous solvent, preferred alcohol water or acetone water.
In the step (5), the consumption of described ethyl acetate is 1～3 times of volume of eluent.
In the step (6), described drying can be vacuum drying, spray drying or lyophilization.
Adopt method for preparing to obtain the Radix Gei japonici antineoplastic extract; Prepared Radix Gei japonici antineoplastic extract effective ingredient (Polyphenols) content can reach 80～90%.
This Radix Gei japonici antineoplastic extract combines with the acceptable drug carrier to make as active component and can be used for treating the oral of various tumors or injection folk prescription and compound preparation, as being the tablet made of adjuvant with starch etc., being the capsule made of adjuvant with the microcrystalline Cellulose, being the injection made of support etc. with mannitol.
The present invention compared with prior art has following advantage and effect:
(1) the present invention to raw medicinal material adopt high temperature, repeatedly, long-time lixiviate, extracting effect is good, antitumor effective ingredient rate of transform height in the raw medicinal material;
(2) technology is simple, and settling step has adopted flocculent precipitation, and that can avoid alcohol deposition method reclaims the processing step that increases because of precipitating the loss and the ethanol that not exclusively cause;
(3) the product purity height is convenient to suitability for industrialized production, adopts the acid adjustment method to remove acid non-soluble substance on the one hand, improves the macroporous resin absorption effect; Adopt purification by macroporous resin Radix Gei japonici antineoplastic effective site on the other hand, and adopt the ethyl acetate extraction method further to improve the purity of effective site, improve active constituent content and precision.
The specific embodiment
Below in conjunction with embodiment the present invention is done further detailed description, but embodiments of the present invention are not limited thereto.
Embodiment 1
With the Radix Gei japonici medical material of 1Kg, add the water of 10L, remained under 100 ℃ of temperature lixiviate 120 minutes, filter then and obtain leachate for the first time; The water that adds 5L again in filtering residue remained under 100 ℃ of temperature lixiviate 90 minutes, filtered then and obtained leachate for the second time; Merge leachate twice.
It heavily is 1.05 that leachate is evaporated to ratio of specific heat, adds chitin 1g, stirs the centrifugal precipitation of going in back, obtains extracting solution; With 10% hydrochloric acid the pH value of extracting solution is transferred to 2, leave standstill then, the centrifugal precipitation of going, obtain supernatant; Supernatant is crossed macroporous resin D101, use 70% ethanol water elution then,, use the ethyl acetate extraction 3 times of 1 times of volume then again with the eluent decompression recycling ethanol; At last the extract normal pressure is concentrated, vacuum drying, make the Radix Gei japonici antineoplastic extract of 30g.Its polyphenol content is 85% after measured.
Embodiment 2
The Radix Gei japonici pulverizing medicinal materials of 1Kg is become coarse powder, add the water of 8L, remained under 80 ℃ of temperature lixiviate 60 minutes, filter then and obtain leachate for the first time; The water that adds 4L again in filtering residue remained under 80 ℃ of temperature lixiviate 60 minutes, filtered then and obtained leachate for the second time; Merge leachate twice.
Leachate is added gelatin 10g, stir the centrifugal precipitation of going in back, obtain extracting solution; With 20% acetic acid the pH value of extracting solution is transferred to 5, leave standstill then, the centrifugal precipitation of going, obtain supernatant; Supernatant is crossed macroporous resin HPD300, use 70% acetone water elution then,, use the ethyl acetate extraction 1 time of 3 times of volumes then again with eluent reclaim under reduced pressure acetone; With extract concentrating under reduced pressure, lyophilization, make the Radix Gei japonici antineoplastic extract of 35g at last.Its polyphenol content is 87% after measured.
Embodiment 3
The Radix Gei japonici pulverizing medicinal materials of 1Kg is become coarse powder, add the water of 9L, remained under 90 ℃ of temperature lixiviate 90 minutes, filter then and obtain leachate for the first time; The water that adds 5L again in filtering residue remained under 90 ℃ of temperature lixiviate 90 minutes, filtered then and obtained leachate for the second time; The water that adds 5L again in filtering residue remained under 90 ℃ of temperature lixiviate 90 minutes, filtered then and obtained leachate for the third time; Merge three times leachate.
The leachate normal pressure is concentrated into ratio of specific heat weighs 1.03, add ZTC flocculating agent 3g, stir the centrifugal precipitation of going in back, obtain extracting solution; With 5% hydrochloric acid the pH value of extracting solution is transferred to 3, leave standstill then, the centrifugal precipitation of going, obtain supernatant; Supernatant is crossed macroporous resin HPD600, use 50% acetone water elution then,, use the ethyl acetate extraction 3 times of 2 times of volumes then again with eluent reclaim under reduced pressure acetone; At last the extract normal pressure is concentrated, spray drying, make the Radix Gei japonici antineoplastic extract of 36g.Its polyphenol content is 83% after measured.
Embodiment 4
The Radix Gei japonici pulverizing medicinal materials of 1Kg is become coarse powder, add 70% ethanol of 10L, remained under 70 ℃ of temperature lixiviate 90 minutes, filter then and obtain leachate for the first time; 70% ethanol that adds 5L again in filtering residue remained under 70 ℃ of temperature lixiviate 60 minutes, filtered then and obtained leachate for the second time; Merge leachate twice.
Leachate is evaporated to ratio of specific heat weighs 1.05, add chitin 3g, stir the centrifugal precipitation of going in back, obtain extracting solution; With 5% hydrochloric acid the pH value of extracting solution is transferred to 2, leave standstill then, the centrifugal precipitation of going, obtain supernatant; Supernatant is crossed macroporous resin HPD300, use 50% ethanol water elution then,, use the ethyl acetate extraction 2 times of 3 times of volumes then again with the eluent decompression recycling ethanol; With extract concentrating under reduced pressure, lyophilization, make the Radix Gei japonici antineoplastic extract of 38g at last.Its polyphenol content is 93% after measured.
Embodiment 5
The Radix Gei japonici pulverizing medicinal materials of 1Kg is become coarse powder, add 50% ethanol of 8L, remained under 80 ℃ of temperature lixiviate 60 minutes, filter then and obtain leachate for the first time; 50% ethanol that adds 4L again in filtering residue remained under 80 ℃ of temperature lixiviate 60 minutes, filtered then and obtained leachate for the second time; 50% ethanol that adds 4L again in filtering residue remained under 80 ℃ of temperature lixiviate 60 minutes, filtered then and obtained leachate for the third time; Merge three times leachate.
The leachate normal pressure is concentrated into ratio of specific heat weighs 1.03, add gelatin 8g, stir the centrifugal precipitation of going in back, obtain extracting solution; With 20% acetic acid the pH value of extracting solution is transferred to 4, leave standstill then, the centrifugal precipitation of going, obtain supernatant; Supernatant is crossed macroporous resin HPD600, use 70% acetone water elution then,, use the ethyl acetate extraction 2 times of 2 times of volumes then again with eluent reclaim under reduced pressure acetone; At last the extract normal pressure is concentrated, spray drying, make the Radix Gei japonici antineoplastic extract of 36g.Its polyphenol content is 96% after measured.
Embodiment 6
With the Radix Gei japonici medical material of 1Kg, add 30% ethanol of 9L, remained under 80 ℃ of temperature lixiviate 120 minutes, filter then and obtain leachate for the first time; 30% ethanol that adds 5L again in filtering residue remained under 80 ℃ of temperature lixiviate 90 minutes, filtered then and obtained leachate for the second time; Merge leachate twice.
In leachate, add ZTC flocculating agent 5g, stir the centrifugal precipitation of going in back, obtain extracting solution; With 10% hydrochloric acid the pH value of extracting solution is transferred to 3, leave standstill then, the centrifugal precipitation of going, obtain supernatant; Supernatant is crossed macroporous resin D101, use 70% ethanol water elution then,, use the ethyl acetate extraction 3 times of 1 times of volume then again with the eluent decompression recycling ethanol; At last the extract normal pressure is concentrated, vacuum drying, make the Radix Gei japonici antineoplastic extract of 32g.Its polyphenol content is 90% after measured.
Embodiment 7
The cell experiment of Radix Gei japonici antineoplastic extract:
Method: with tumor cell (2 * 10
6/ ml), put into 96 aseptic hole culture plates, every hole adds tumor cell suspension 50 μ l, add the RPMI-1640 50 μ l that contain 15% calf serum, the aqueous solution 10 μ l that add the Radix Gei japonici antineoplastic extract of embodiment 1～3 preparation, every group 3 hole adds the normal saline vibration mixing of equivalent in the hole, put into 5% CO then
2Incubator (37 ℃) is cultivated 24h, adds tetrazolium salts (MTT) phosphate buffer, and every hole 10 μ l (every ml contains MTT5mg) continue to cultivate 4h, add dimethylsulfone 100ml then, cessation reaction is with enzyme glue immune detection instrument, respectively at wavelength 570nm, the 630nm place measures optical density OD value, result of calculation.
Computing formula:
Result: in gastric cancer RF cell strain and the experiment of rectal cancer LS174T cell strain tumor cell culture, find, the Radix Gei japonici antineoplastic extract is significantly higher than the water extract (not passing through the water extracting liquid of precipitation, acid adjustment, macroporous resin adsorption and extraction) of its medical material to the suppression ratio of two kinds of tumors, and is dosage escalation.The results are shown in subordinate list 1.
Subordinate list 1 Radix Gei japonici effective site is to the inhibition experiment of gastric cancer and colorectal cancer cells
| Handle | Concentration (mg/ml) | To gastric cancer suppression ratio % (x ± SD) | To colon cancer suppression ratio % (x ± SD) |
| The aqueous solution of the aqueous solution embodiment 3 of the aqueous solution embodiment 2 of the water extract embodiment 1 of Radix Gei japonici medical material | ????5 ????1 ????2 ????5 | ????23.5±4.5 ????43.7±6.2 ????72.7±4.9 ????96.3±8.4 | ????25.3±5.7 ????45.3±5.1 ????76.8±6.4 ????95.9±9.3 |
Embodiment 8
The Radix Gei japonici compound effective component suppresses the zoopery of tumor
Method: get mice S180 tumor piece, dilution and adjustment cell number (1 * 10 after the homogenate
7/ ml), it is subcutaneous to inject the right axil of mice with 0.2ml, and random packet administration after 24 hours wherein, is organized gastric infusion in contrast with normal saline; CTX (cyclophosphamide) organizes intraperitoneal administration; The Radix Gei japonici antineoplastic extract of water extract of Radix Gei japonici medical material (not passing through the water extracting liquid of precipitation, acid adjustment, macroporous resin adsorption and extraction) and embodiment 3,6 preparations is gastric infusion, the execution mice of weighing in the 11st day is got the tumor piece, claim that tumor is heavy and survey the tumor volume, calculate tumour inhibiting rate.
The result: the water extract of Radix Gei japonici medical material is 38.3% to the S180 tumour inhibiting rate, and the tumour inhibiting rate of the Radix Gei japonici antineoplastic extract of isodose the present invention's preparation is 66.4%, there were significant differences with matched group, and not remarkable with the difference of CTX group, points out it to have good antitumor action.The results are shown in subordinate list 2.
Subordinate list 2 Radix Gei japonici effective sites are to the inhibition zoopery of S180 sarcoma
| Grouping | Dosage (g/Kg) | Tumor heavy (g) (x ± SD) | Tumor volume (cm 3) ???( x±SD) | Tumour inhibiting rate (%) |
| Water extract embodiment 3 embodiment 6 of matched group CTX group Radix Gei japonici medical material | ? ?25 ?20 ?10 ?20 | ????3.42±0.46 ????1.12±0.26 ????2.11±0.26 ????1.45±0.41 ????1.15±0.29 | ???2.85±0.73 ???0.81±0.37 ???2.01±0.28 ???1.49±0.52 ???0.89±0.23 | ? ???67.2 ???38.3 ???51.2 ???66.4 |
Claims (10)
1, a kind of preparation method of Radix Gei japonici antineoplastic extract is characterized in that comprising the steps:
(1) water or organic solvent lixiviate Radix Gei japonici medical material under 70～100 ℃ of temperature obtains leachate;
(2) add flocculating agent in leachate, the precipitation roguing obtains extracting solution;
(3) pH value with extracting solution is adjusted to 2～5, leaves standstill, and obtains supernatant;
(4) supernatant is crossed macroporous resin, use solvent elution, collect eluent;
(5) eluent is reclaimed solvent after, use ethyl acetate extraction, obtain extract;
(6), obtain the Radix Gei japonici antineoplastic extract with the extract concentrate drying.
2, the preparation method of Radix Gei japonici antineoplastic extract according to claim 1 is characterized in that: in the step (1), described organic solvent is 30～70% ethanol.
3, the preparation method of Radix Gei japonici antineoplastic extract according to claim 1 is characterized in that: in the step (1), extraction temperature is 70～80 ℃.
4, the preparation method of Radix Gei japonici antineoplastic extract according to claim 1 is characterized in that: in the step (1), described lixiviate number of times is 1～3 time; Each extraction time is 1～2 hour; The Radix Gei japonici of lixiviate for the first time and the weight ratio of water or organic solvent are 1: 8～10; Be 1: 4～5 with the Radix Gei japonici of lixiviate for the third time and the weight ratio of water or organic solvent for the second time.
5, the preparation method of Radix Gei japonici antineoplastic extract according to claim 1 is characterized in that: in the step (2), the consumption of described flocculating agent is 0.1～1% of a Radix Gei japonici medical material weight.
6, the preparation method of Radix Gei japonici antineoplastic extract according to claim 1 is characterized in that: in the step (3), adopt hydrochloric acid or acetic acid to regulate the pH value of extracting solution.
7, the preparation method of Radix Gei japonici antineoplastic extract according to claim 1 is characterized in that: in the step (4), described solvent is ethanol water or acetone water.
8, the preparation method of Radix Gei japonici antineoplastic extract according to claim 1 is characterized in that: in the step (5), the consumption of described ethyl acetate is 1～3 times of volume of eluent.
9, a kind of Radix Gei japonici antineoplastic extract according to each described method preparation of claim 1～8.
10, the application of Radix Gei japonici antineoplastic extract according to claim 9 is characterized in that: the Radix Gei japonici antineoplastic extract is combined with the acceptable drug carrier to make as active component be used for the treatment of the oral of various tumors or injection folk prescription and compound preparation.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100340251C (en) * | 2006-03-09 | 2007-10-03 | 中国林业科学研究院林产化学工业研究所 | Method for extracting anti-oxygenation active material from Chinese waxmyrtle Bark |
| CN100383525C (en) * | 2005-12-02 | 2008-04-23 | 云南白药集团股份有限公司 | Identifying method for Red-bayberry root herb |
| CN100383522C (en) * | 2005-12-02 | 2008-04-23 | 云南白药集团股份有限公司 | Red-bay berry root herb content detecting method |
| CN103520651A (en) * | 2013-10-25 | 2014-01-22 | 刘瑾 | Traditional Chinese medicine composition for treating cervical cancer |
| CN111686085A (en) * | 2020-06-30 | 2020-09-22 | 贵州益佰女子大药厂有限责任公司 | Preparation method of throat-clearing preparation |
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2005
- 2005-01-04 CN CNB2005100326651A patent/CN100546601C/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100383525C (en) * | 2005-12-02 | 2008-04-23 | 云南白药集团股份有限公司 | Identifying method for Red-bayberry root herb |
| CN100383522C (en) * | 2005-12-02 | 2008-04-23 | 云南白药集团股份有限公司 | Red-bay berry root herb content detecting method |
| CN100340251C (en) * | 2006-03-09 | 2007-10-03 | 中国林业科学研究院林产化学工业研究所 | Method for extracting anti-oxygenation active material from Chinese waxmyrtle Bark |
| CN103520651A (en) * | 2013-10-25 | 2014-01-22 | 刘瑾 | Traditional Chinese medicine composition for treating cervical cancer |
| CN111686085A (en) * | 2020-06-30 | 2020-09-22 | 贵州益佰女子大药厂有限责任公司 | Preparation method of throat-clearing preparation |
| CN111686085B (en) * | 2020-06-30 | 2022-09-06 | 贵州益佰女子大药厂有限责任公司 | Preparation method of throat clearing preparation |
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