CN100340251C - Method for extracting anti-oxygenation active material from Chinese waxmyrtle Bark - Google Patents

Method for extracting anti-oxygenation active material from Chinese waxmyrtle Bark Download PDF

Info

Publication number
CN100340251C
CN100340251C CNB2006100387397A CN200610038739A CN100340251C CN 100340251 C CN100340251 C CN 100340251C CN B2006100387397 A CNB2006100387397 A CN B2006100387397A CN 200610038739 A CN200610038739 A CN 200610038739A CN 100340251 C CN100340251 C CN 100340251C
Authority
CN
China
Prior art keywords
water
solution
extraction
bark
active substance
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CNB2006100387397A
Other languages
Chinese (zh)
Other versions
CN1850133A (en
Inventor
陈笳鸿
汪咏梅
吴冬梅
吴在嵩
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institute of Chemical Industry of Forest Products of CAF
Original Assignee
Institute of Chemical Industry of Forest Products of CAF
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institute of Chemical Industry of Forest Products of CAF filed Critical Institute of Chemical Industry of Forest Products of CAF
Priority to CNB2006100387397A priority Critical patent/CN100340251C/en
Publication of CN1850133A publication Critical patent/CN1850133A/en
Application granted granted Critical
Publication of CN100340251C publication Critical patent/CN100340251C/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Abstract

The present invention relates to a method for extracting natural oxidation protection active substances from Chinese waxmyrtle bark, which comprises the steps: purified Chinese waxmyrtle bark is pulverized by a plant pulverizer; pulverized bark is extracted in water for 60 to 120 min under the condition of the temperature of 50 to 80 DEG C, extracting solution is added with 0.5 to 1% chitosan acetate solution as a flocculating agent, the agitation operation and the standing operation are carried out, and the flocculating agent is cooled to room temperature; the temperature drops to 1 to 7 DEG C, and the standing time is from 24 to 48h, and the clear solution is mixed with water to form column feeding solution whose the solid content is from 4 to 5%w/v; fillers are used for adsorbing and separating non-polar macroporous resin or low polarity macroporous resin which is pre-purified, ethanol is distilled from alcohol water eluent at ordinary pressure, and the obtained product water solution is in vacuum freeze drying so as to obtain powdery natural oxidation protection active substances. The target product prepared by the method has the biological activity functions of strong oxidation protection and free radical clearance.

Description

Extract the method for natural anti-oxidation active substance from the hair Cortex Myricae Rubrae
Technical field
The present invention relates to a kind of method, relate in particular to a kind of method of extracting the natural anti-oxidation active substance from the hair Cortex Myricae Rubrae from vegetable material extraction natural anti-oxidation active substance.
Background technology
Hair Fructus Myricae rubrae (Myrica esculenta) has wide resource distribution at China's southern area.The hair Cortex Myricae Rubrae is rich in plant polyphenol; its main component is the poly-former delphinidin (polymeric prodelphinidins) of local galloylization; this phenomenon is rare in plant kingdom, because many and procyanidin (procyanidins) coexistence at plant Central Plains delphinidin.Former delphinidin and procyanidin belong to proanthocyanidin (proanthocyanidins) class.Studies show that, oligomeric proanthocyanidins (oligomeric proanthocyanidins, OPC) has very strong antioxidation biology activity, can remove harmful free radicals in the human body, improve the immunity of human body, can be used as the main effective ingredient of anti-cancer, mutation, control cardiovascular disease medicine and as the new type natural antioxidant of safety non-toxic and free radical scavenger etc., become the important additives of the important source material of medicine, health product and food, cosmetics.
Abroad to the bioactive research of proanthocyanidin (mainly being procyanidin) existing decades of history, particularly since the eighties, countries such as Germany, France, Italy, Austria, Japan, India, Hungary, Korea S have carried out number of research projects.China has added the ranks of world research exploitation proanthocyanidin from the eighties.
Nineteen fifty-one, France Jacques Masquelier found the antioxygenic property of procyanidin first, and successfully extracted procyanidin from the seashore Cortex Pini Massonianae.U.S. Joslyn in 1967 etc. isolate the proanthocyanidin polyphenolic substance from Pericarpium Vitis viniferae and Semen Vitis viniferae.Bombardelli had invented the method for extracting the high assay proto cyaniding mixture from Semen Vitis viniferae in 1976, and Fructus Vitis viniferae (seed and skin) is subjected to people's attention day by day as the valuable source of procyanidin.Jacques Masquelier in 1979 further studies and extracts procyanidin and realized industrialization from Semen Vitis viniferae.Nineteen eighty-three France D.Cagnian etc. has successfully synthesized the polymer of catechin and epicatechin.Foreign literature also has the report of extraction separation proanthocyanidin from different natural products the same period.
Abroad have about the patent of extracting proanthocyanidin is comparatively representational: French Jacques Masquellier from the maritime pine bark with boiling water extraction, the ethyl acetate extraction crude extract, the chloroform precipitated product was applied for a patent in 1969.It is raw material with Aesculus hippocastanum bark that there is He Minming in Japan, uses water extraction, petroleum ether, resin absorption product.U.S. Tochiakl Ariga from Cortex Pini with methanol extraction, petroleum ether, ethyl acetate extraction, liquid-phase chromatographic column separate and to obtain product, apply for a patent in 1989.Poland OszmianskiJan is a solvent with acetone, adopts ultrasound wave to extract procyanidin from Semen Vitis viniferae, ethyl acetate extraction, and the chloroform precipitated product was applied for a patent in 1996.U.S. Henkel company extracts procyanidin from Semen Vitis viniferae, with boiling water extraction, and the ethyl acetate extraction crude extract, chloroform precipitation product was applied for a patent in 1997.
Current existing multiple procyanidin is as the research report of the Natural antioxidant, and existing health promoting product appearance, for example: from Pinus pinaste bark and the pycnogenol (pycnogenol) that from other Pinus bark, extracts, the procyanidin that from Semen Vitis viniferae and skin, extracts etc.
The research of China's proanthocyanidin mostly is in the fitochemical studies stage generally.In the last few years, domestic extraction and purification process and anti-oxidation function to procyanidin done preliminary research, and manufacturer production Semen Vitis viniferae and Cortex Pini Massonianae extract are also arranged, and came into the market as natural powerful antioxidant.
In sum, proanthocyanidin extraction and separation technology and the active research of antioxidation biology thereof have been become the focus of domestic and international native compound research field, increasingly extensive and deep.But most research and development, mainly concentrate on the natural anti-oxidation active substance based on procyanidin of extraction separation from Semen Vitis viniferae (skin) and Cortex Pini raw material, and the present invention relates to from the natural anti-oxidation active substance of hair Cortex Myricae Rubrae extraction based on former delphinidin.
Summary of the invention
The invention provides a kind of is the method that raw material extracts the natural anti-oxidation active substance with the hair Cortex Myricae Rubrae.
Concrete grammar is as follows:
(1) pulverize: the hair Cortex Myricae Rubrae raw material after will purifying is pulverized sieve aperture Φ=2~4mm with the plant pulverizer;
(2) extract: with water is solvent, and the bark raw material water that crushes under 50~80 ℃ of temperature conditions, is extracted 60~120min, extracting solution; Specifically can select following any method to extract:
Jar group adverse current continuous extraction method: every jar of bark raw material and water that adding crushes, the solid-liquid mass volume ratio is 1: 5~10, and placing extraction temperature is in 60~80 ℃ of thermostatic water bath, and single jar of extraction time is 60~120min, several 4~6 of jar.
Ultrasound-enhanced extraction method: bark that crushes and water place in the reactive tank, and the solid-liquid mass volume ratio is 1: 5~10, and ultrasonic operating frequency is 50~100kHz, ultrasonic power is 100~200W, and extracting temperature is 55~65 ℃, and the time is 60~90min, filter cleaner gets extracting solution.
(3) flocculating sedimentation: extracting solution add concentration be 0.5~1% chitosan acetic acid solution as flocculating agent, stirring is left standstill, and is cooled to room temperature; The consumption of chitosan is 0.025~0.05% (extracting solution can be concentrated into 6~7 ° of Be ' earlier) of bark material quantity;
(4) freeze and separate: the extracting solution that will add flocculating agent is cooled to 1~7 ℃, leaves standstill 24~48h, isolates clear liquid, and precipitate discards;
(5) resin absorption separates: above-mentioned clear liquid water is made into the upper prop liquid that solid content is 4~5%w/v, adopting filler is in advance through the nonpolar or low polar macroporous adsorption resin of purified treatment, the upper prop flow velocity is 1.4~1.7Bv/h, absorption is back with pure washing post, the reuse volume ratio is the flow velocity eluting of the ethanol water of 60~70%v/v with 1.4~1.7Bv/h, collects eluent;
(6) desolventizing: at normal pressure (1.01 * 10 5Pa) from pure water elution liquid, steam ethanol under,, get product water solution with the pure water dilution;
(7) vacuum lyophilization: product water solution carries out dehydrate under the vacuum low-pressure at 0~0.01Mpa after freezing, promptly gets powdery natural anti-oxidation active substance.
Compare with existing bibliographical information from plant resources extraction proanthocyanidin, the present invention has following advantage:
1. the current research relevant with proanthocyanidin both at home and abroad mainly concentrates on from Semen Vitis viniferae, skin and Cortex Pini raw material extraction separation based on the natural anti-oxidation active substance of procyanidin, and the present invention is from the natural anti-oxidation active substance of hair Cortex Myricae Rubrae extraction based on former delphinidin.The hair Fructus Myricae rubrae has wide resource distribution at China's southern area, is rich in former delphinidin in the bark, and therefore target product raw material sources of the present invention are abundant.
2. can analyze deduction by the similarities and differences of the chemical structure characteristic of former delphinidin and procyanidin, the present invention from the hair Cortex Myricae Rubrae extract based on the polyphenolic substance of former delphinidin with compare with the polyphenolic substance that Semen Vitis viniferae extracts from Cortex Pini based on procyanidin, have the bioactive functions of stronger antioxidation, removing free radical.
Proanthocyanidin is meant a class plant polyphenol that forms, can produce anthocyanidin through hot acid-pure processing in plant.Proanthocyanidin is pressed dissimilar that hydroxyl replaces in the molecule, and it is multiple to be divided into procyanidin, former delphinidin, former acacetin (prorobinetinidins), former luxuriant and rich with fragrance plucked instrument element (profisetinidins) etc.The chemical constitution difference of procyanidin and former delphinidin is: the former molecular structure component units is catechin (1), and the latter's molecular structure component units is Gallate catechin (2).
(1) the molecular structure component units of the former delphinidin of structure component units (2) of former colored cream element
(3) poly-former delphinidin
The component units flavan-3-alcohol structure of the poly-former delphinidin (3) that extracts from the hair Cortex Myricae Rubrae, last 3,4,5 of B ring has the vicinal hydroxyl groups that 3 performances are enlivened very much; Its another feature is local galloylization: the unit, bottom is epigallocatechin-3-O-gallate fat, galloyl degree about 40%.
Though procyanidin, former delphinidin belong to proanthocyanidins, the difference that the difference on the molecular structure will inevitably cause its antioxidation and remove the free radical activity power.Whether phenolic compound has the ability of catching free radical, depend on that can its phenolic hydroxyl group as H atom donor, thereby generate the stable chemical compound of conjugation with combined with radical, the blocking-up free chain reaction, its ability of removing free radical and its molecular structure (PheO-H bond dissociation energy, PheO conjugation delocalization, sterically hindered etc.) and molecular weight thereof are relevant.That is hydroxyl value and mutual alignment play an important role to its biological activity in the molecular structure of procyanidin and former delphinidin, and adjacent phenolic hydroxyl group biological activity at most is strong.
3. in addition, from the polyphenolic substance that the hair Cortex Myricae Rubrae extracts, also contain a small amount of other polyphenolic substance such as castalagin, gallic acid, epigallocatechin-3-O-gallic acid ester, epigallocatechin-3-O-galloyl-epigallocatechin etc., all have the biological activity of good antioxidation, removing free radical, also can play synergism the former delphinidin of main component.
The specific embodiment
Embodiment 1:
A kind of method of extracting the natural anti-oxidation active substance from the hair Cortex Myricae Rubrae:
(1) pulverizes, the hair Cortex Myricae Rubrae raw material after purifying is pulverized (Φ 2~4mm sieve aperture) with the plant pulverizer;
(2) extracting, is solvent with water, and the bark raw material water that crushes under 50~80 ℃ of constant temperatures, is extracted 60~120min, extracting solution; Wherein temperature can be: 55 ℃, 60 ℃, 65 ℃, 70 ℃, 80 ℃.
(3) flocculating sedimentation, extracting solution add concentration be 0.5~1% chitosan acetic acid solution as flocculating agent, stirring is left standstill, and is cooled to room temperature; The consumption of chitosan is 0.025~0.05% of a bark material quantity; Wherein the concentration of chitosan acetic acid solution can be: 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%.
(4) freeze and separate is cooled to 1~7 ℃ with the extracting solution that adds flocculating agent, leaves standstill 24~48h, isolates clear liquid, and precipitate discards;
(5) resin absorption separates, above-mentioned clear liquid water is made into the upper prop liquid that solid content is 4~5%w/v, adopting filler is in advance through the nonpolar or low polar macroporous adsorption resin of purified treatment, the upper prop flow velocity is 1.4~1.7Bv/h, absorption is back with pure washing post, the reuse volume ratio is the flow velocity eluting of the ethanol water of 60~70%v/v with 1.4~1.7Bv/h, collects eluent;
(6) desolventizing is under normal pressure (1.01 * 10 5Pa) from pure water elution liquid, steam ethanol,, get product water solution with the pure water dilution;
(7) vacuum lyophilization, product water solution are carried out dehydrate under the vacuum low-pressure at 0~0.01Mpa after freezing, promptly get powdery natural anti-oxidation active substance.
Embodiment 2:
Will through purify, air-dry and pulverize and (use the plant pulverizer, by Φ 2mm sieve plate) the hair Cortex Myricae Rubrae be loaded in the lixiviate jar, totally 6 jars, every canned raw material 50g and water 500mL, place in 80 ℃ of thermostatic water bath, by the adverse current extraction, every jar of extraction time is 60min, shared raw material 300g, fluid 2500mL;
2. extracting solution is concentrated into 550mL (measured specific gravity is 4.9 ° of Be '), adds concentration and be 1% chitosan acetic acid solution 15mL, leave standstill after the strong mixing while hot, be chilled to be placed in 7 ℃ of refrigerators after the room temperature and leave standstill 48h, tell clear liquid 534mL (5.2 ° of Be ');
3. above-mentioned clear liquid being diluted to concentration with pure water is 5% (w/v), as upper prop liquid it is divided into 2 parts, adds to two respectively with the flow velocity of 1.4Bv/h and has passed through pretreated AB-8 type macroporous adsorptive resins (Bv=250cm 3), then use the pure washing post of 2.4Bv/h respectively, use 70% ethanol water 430mL flow velocity eluting more respectively with 1.7Bv/h, merge eluent;
4. under normal pressure (1.01 * 10 5Pa) steam ethanol (reuse) in the ethanol water elution liquid, with the pure water dilution, product water solution;
5. with the vacuum lyophilization at low temperatures of product water solution, obtain natural anti-oxidation active substance powder product 35g, yield is 14.3% (the bark raw material is by over dry).
Embodiment 3:
1. will place in the KH-200DB type numerical control ultrasonic generator groove by embodiment 2 methods pretreated hair Cortex Myricae Rubrae 350g and lixiviate water 2500mL, under ultrasound-enhanced effect, extract, supersonic frequency is 50kHz, ultrasonic power is 200W, the control temperature is at 60 ± 5 ℃, and extraction time is 60min, and then take out the back, filter and remove residue gets extracting solution 2150mL;
2. add 17.5mL concentration and be 1% chitosan acetic acid solution in extracting solution, leave standstill after the strong mixing and be chilled to room temperature, put into and tell clear liquid 2020mL after 5 ℃ of refrigerators leave standstill 24h, surveying its solid content is 53.7g;
3. above-mentioned clear liquid is concentrated into 1080mL, surveying its concentration is 5% (w/v);
4. above-mentioned solution is divided into 3 parts and goes up the macroporous resin adsorption post, the upper prop liquid of every post is 360mL;
5. press the operation of (3) among the embodiment 2, (4), (5), obtain natural anti-oxidation active substance powder product 36.8g, yield is 12.9% (the bark raw material is by over dry).
The antioxidant activity of target product obtains checking (seeing attached 1, attached 2, attached 3) by test among the present invention: consaturated oil is had the antioxidative effect; Metal ion to the catalyzed oil lipid peroxidation has complexing power; The ability of removing free radical is arranged.
Attached 1: target product is measured the antioxygenic property of consaturated oil among the present invention
Adopt the inhibition ability of Schaal oven method measuring target product to the Vegetable oil lipoprotein thermal oxide.The ratio that adds the 0.4g antioxidant in 1kg substrate oil, take by weighing the target product sample 12mg of preparation among the embodiment 2, in container, be dissolved in 60% ethanol of 3mL, add 30g substrate oleum lini then, add 1 Span 80 again, vibration mixes makes it fully emulsified, places in 60 ± 2 ℃ of baking ovens, the 3g that draws oil sample at regular intervals measures its peroxide value POV (meq/kg) with iodimetric titration (GB/T 5538-1995 " oil peroxidation pH-value determination pH method ").The colleague is blank.The result is as follows:
Result data shows that target product has the antioxidation to consaturated oil.
Attached 2: target product is measured the complex performance of metal ion among the present invention
Target product energy and Fe 2+Ion generates colored complex, at wavelength 584nm place characteristic absorption peak is arranged with spectrophotometer, and its absorbance is directly proportional with the concentration of complex.
Take by weighing the target product sample 0.2g (being accurate to 0.1mg) of preparation among the embodiment 2, be dissolved in the 200mL distilled water, its concentration is 1mg/mL, takes by weighing O.1g (being accurate to 0.1mg) FeSO 47H 2O is dissolved in the 100mL distilled water Fe 2+Concentration is 0.2mg/mL.
In the sample solution of 5mL, add the Fe of O, 1,1.5,2.0,2.5,3.O, 3.5,4.0,4.5mL respectively 2+Solution is settled to 10mL with distilled water respectively again, measures absorbance with spectrophotometer respectively at the 584nm place, works as Fe 2+The solution addition increases to absorbance when keeping constant, shows that sample solution is to Fe 2+Ionic complexation is saturated.
Calculate target product thus to Fe 2+Ionic complexing power is 181mg/g.
Result data shows that target product has the complexing power to metal ion.
Attached 3: target product is measured the removing ability of free radical among the present invention
(DPPH, U.S. Sigma company) is reference material with diphenyl-picryl-diazanyl free radical, with the clearance rate of spectrophotometry target product of the present invention to DPPH.
The preparation working solution:
The alcoholic solution of DPPH, concentration are 0.025mg/mL; The alcoholic solution of target product sample, concentration are 0.07mg/mL.
Spectrophotometric determination (λ=517nm; The 1cm cuvette):
The original absorbance of DPPH solution: DPPH solution 5mL adds ethanol 1mL, absorbance 0.485.
The DPPH solution absorbency situation of change that adds the target product sample: DPPH solution 5mL adds sample solution 1mL, promptly begin to measure absorbance after the mixing, every subsequently interval 1min measures 1 time, and absorbance continues to descend, till maintenance was invariable, timing was also calculated.
Measurement result:
Absorbance drops to the time of steady state value: 7min;
DPPH clearance rate: 92.0%;
DPPH clearance rate: 0.046mg/min.
Result data shows that target product has the removing ability to free radical (DPPH).

Claims (5)

1. one kind is extracted the method for natural anti-oxidation active substance from the hair Cortex Myricae Rubrae, it is characterized in that:
(1) pulverize: the hair Cortex Myricae Rubrae raw material after will purifying is pulverized with the plant pulverizer;
(2) extract: with water is solvent, and the bark raw material water that crushes under 50~80 ℃ of temperature conditions, is extracted 60~120min, extracting solution;
(3) flocculating sedimentation: extracting solution add concentration be 0.5~1% chitosan acetic acid solution as flocculating agent, stirring is left standstill, and is cooled to room temperature; The consumption of chitosan is 0.025~0.05% of a bark material quantity;
(4) freeze and separate: the extracting solution that will add flocculating agent is cooled to 1~7 ℃, leaves standstill 24~48h, isolates clear liquid, and precipitate discards;
(5) resin absorption separates: above-mentioned clear liquid water is made into the upper prop liquid that solid content is 4~5%w/v, adopting filler is in advance through the nonpolar or low polar macroporous adsorption resin of purified treatment, the upper prop flow velocity is 1.4~1.7Bv/h, absorption is back with pure washing post, the reuse volume ratio is the flow velocity eluting of the ethanol water of 60~70%v/v with 1.4~1.7Bv/h, collects eluent;
(6) desolventizing: under normal pressure, from pure water elution liquid, steam ethanol,, get product water solution with the pure water dilution;
(7) vacuum lyophilization: product water solution carries out dehydrate under the vacuum low-pressure at 0~0.01Mpa after freezing, promptly gets powdery natural anti-oxidation active substance.
2. a kind of method from hair Cortex Myricae Rubrae extraction natural anti-oxidation active substance according to claim 1 is characterized in that: the sieve aperture Φ=2~4mm of pulverizer in the step (1).
3. a kind of method according to claim 1 from hair Cortex Myricae Rubrae extraction natural anti-oxidation active substance, it is characterized in that: step (2) is extracted with following manner:
Jar group adverse current continuous extraction method: every jar of bark raw material and water that adding crushes, the solid-liquid mass volume ratio is 1: 5~10, and placing extraction temperature is in 60~80 ℃ of thermostatic water bath, and single jar of extraction time is 60~120min, several 4~6 of jar.
4. a kind of method according to claim 1 from hair Cortex Myricae Rubrae extraction natural anti-oxidation active substance, it is characterized in that: step (2) can also be extracted with following manner:
Ultrasound-enhanced extraction method: bark that crushes and water place in the reactive tank, and the solid-liquid mass volume ratio is 1: 5~10, and ultrasonic operating frequency is 50~100kHz, and ultrasonic power is 100~200W, and extracting temperature is 55~65 ℃, and the time is 60~90min.
5. a kind of method from hair Cortex Myricae Rubrae extraction natural anti-oxidation active substance according to claim 1, it is characterized in that: step (3) flocculating sedimentation, extracting solution can be concentrated into 6~7 ° of Be ' earlier.
CNB2006100387397A 2006-03-09 2006-03-09 Method for extracting anti-oxygenation active material from Chinese waxmyrtle Bark Expired - Fee Related CN100340251C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CNB2006100387397A CN100340251C (en) 2006-03-09 2006-03-09 Method for extracting anti-oxygenation active material from Chinese waxmyrtle Bark

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CNB2006100387397A CN100340251C (en) 2006-03-09 2006-03-09 Method for extracting anti-oxygenation active material from Chinese waxmyrtle Bark

Publications (2)

Publication Number Publication Date
CN1850133A CN1850133A (en) 2006-10-25
CN100340251C true CN100340251C (en) 2007-10-03

Family

ID=37131645

Family Applications (1)

Application Number Title Priority Date Filing Date
CNB2006100387397A Expired - Fee Related CN100340251C (en) 2006-03-09 2006-03-09 Method for extracting anti-oxygenation active material from Chinese waxmyrtle Bark

Country Status (1)

Country Link
CN (1) CN100340251C (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102643259B (en) * 2012-04-11 2014-04-16 浙江大学 Ultrasonic preparation method of myrica rubra leaf proanthocyanidin oligomer

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1679795A (en) * 2005-01-04 2005-10-12 华南理工大学 Anti-cancer extracts from thinleaf adina root and its making method and use

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1679795A (en) * 2005-01-04 2005-10-12 华南理工大学 Anti-cancer extracts from thinleaf adina root and its making method and use

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
FLAVONOLS FROM MYRICA ESCULENTA BARK Sun Dawang,林产化学与工业,第11卷第4期 1991 *
毛杨梅及油柑树皮单宁组分的研究 赵祖春等,林产化学与工业,第7卷第3期 1987 *

Also Published As

Publication number Publication date
CN1850133A (en) 2006-10-25

Similar Documents

Publication Publication Date Title
Xu et al. Characteristics and bioactivities of different molecular weight polysaccharides from camellia seed cake
CN103494862B (en) Method for extracting olive polyphenol from olive processing waste liquor
CN105267275B (en) Method for extracting flavone from chrysanthemum
CN102210786B (en) Method for extracting natural antioxidant from shells of camellia oleifera
CN107362080B (en) A kind of extracting method of polyphenol in passion fruit pericarp
CN101239962B (en) Method for extracting proanthocyanidins from cranberry
CN101204417B (en) Method of extracting natural oxidationresistant active substanceoil from orange tree bark
CN101301319A (en) Preparation of granada flower polyphenol and uses thereof
Min et al. Antioxidative and anti-inflammatory activities of Citrus unshiu peel extracts using a combined process of subcritical water extraction and acid hydrolysis
CN108578317B (en) Method for extracting active ingredients from bamboo leaves and skin care application of active ingredients
CN1546483A (en) Method for extracting high purity seabuckthorn flavone aglycone
Chuo et al. A Glimpse into the extraction methods of active compounds from plants
CN100340251C (en) Method for extracting anti-oxygenation active material from Chinese waxmyrtle Bark
CN104000935B (en) A kind of method that anti-oxidant phenolic acid is extracted in the slag from jacket
CN104983915A (en) Preparation method of natural lycium ruthenicum composite antioxidant
CN110066305B (en) Mechanochemical extraction method for preparing crude naphthopyrone extract from berchemilla lineate
CN101066960A (en) Production process of extracting isoflavone from fern
CN100581535C (en) Method for extracting and purifying flavonoids from apple powder agent and application thereof
CN102362876A (en) Chinese redwood bark extract, preparation method thereof and purpose thereof
CN103393785A (en) Method for preparing malus baccata leaf alcohol extract, and antioxidation and liver protection effects thereof
Yin et al. Study on separation and purification of oligomeric proanthocyanidin from Rhodiola rosea
CN106924117A (en) The washing product composition prepared with remaining artemisia annua residue after extraction qinghaosu
CN107468746A (en) A kind of method that middle extraction polyphenol and flavones are spent from Zhu Ying
Ye et al. Free radical scavenging activity and anti-inflammatory property of the saponin from seeds of Camellia oleifera abel
Chimsook et al. Withdrawn article–Effect of Ultrasonic-Assisted Extraction on Phenolic Content of Freshwater Macroalgae in Northern Thailand

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
C17 Cessation of patent right
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20071003

Termination date: 20110309