CN100383525C - Identifying method for Red-bayberry root herb - Google Patents
Identifying method for Red-bayberry root herb Download PDFInfo
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- CN100383525C CN100383525C CNB2005100486650A CN200510048665A CN100383525C CN 100383525 C CN100383525 C CN 100383525C CN B2005100486650 A CNB2005100486650 A CN B2005100486650A CN 200510048665 A CN200510048665 A CN 200510048665A CN 100383525 C CN100383525 C CN 100383525C
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- myricetrin
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Abstract
The invention relates to a qualitative analysis method for the effective component in natural vegetable drug. It uses myricetrin as reference substance to identify waxberry root drug, and adopts thin-layer chromatography to take identification. It includes the following steps: making sample solution, making reference solution, taking thin-layer chromatography identification. The invention has simple process method, rood repeatability, and strong specialization.
Description
Technical field
The present invention relates to a kind of method for qualitative analysis of natural plant crude drugs effective ingredient.
Background technology
Chinese waxmyrtle root is a kind of Chinese medicine medicinal material commonly used, applies in the multiple Chinese patent drug, as thousand purplish red particles, thousand purplish red capsules etc.But this medicinal material never has the strong discrimination method of a kind of specificity.
Summary of the invention
It is the discrimination method of the Red-bayberry root herb of reference substance with the myricetrin that purpose of the present invention aims to provide a kind of.
The discrimination method of Red-bayberry root herb of the present invention adopts thin-layered chromatography to carry out qualitative identification for being reference substance with the myricetrin.
The discrimination method of Red-bayberry root herb of the present invention is made up of following steps:
One, need testing solution preparation:
Get Red-bayberry root herb meal 2g, add the ethyl acetate of 30ml, sonicated 5 minutes filters, the filtrate evaporate to dryness, and the methyl alcohol that residue adds 2ml makes dissolving;
Two, reference substance solution preparation:
Get myricetrin, add methyl alcohol and make the solution that every 1ml contains 1mg;
Three, thin-layered chromatography is differentiated
Draw each 2 μ l of above-mentioned two kinds of solution, putting respectively on same polyamide film, is developping agent with isopropyl alcohol-water 3:1-3, launches, take out, dry, spray is with 1% aluminium choride ethanolic solution, and it is clear that hot blast blows to the spot colour developing, under ultraviolet lamp, inspect, in the test sample chromatogram, with reference substance chromatogram relevant position on, show the fluorescence spot of same color.
Myricetrin thin layer Study on Identification:
This product meal 2g is got in the preparation of need testing solution, adds ethyl acetate 30ml, and sonicated (power 250W, frequency 25KHZ) 5 minutes filters, and filtrate evaporate to dryness, residue add methyl alcohol 2ml makes dissolving, as need testing solution.
The thin layer plate polyamide film, specification 50 * 100mm; Road and bridge tetramethyl biochemical plastic molding and processing plant in Taizhou, Zhejiang Province city produces specification 100 * 200mm.
Developping agent methanol-water (4: 1); Isopropyl alcohol-water (3: 2); Methenyl choloride-methyl alcohol (7: 3); Normal butyl alcohol-alcohol-water (4: 1: 5); The point sample amount is respectively 1,2,5,10 μ l.Launch: ascending development, exhibition is apart from 6～9cm.
Developer 1% aluminium choride ethanolic solution.Room temperature is 18～25 ℃; Humidity is 65～80%.
Test shows that the preparation method of need testing solution is simple and easy to do, adopts commercially available polyamide film, is developping agent with isopropyl alcohol-water (3: 2), and the thin-layer chromatography spot degree of separation that obtains is better, and the point sample amount is 1～3 μ l, and the thin-layer chromatography spot degree of separation that obtains is better.
Myricetrin contains a plurality of phenolic hydroxyl groups for the flavonols composition, after the aluminium choride colour developing, and displaing yellow fluorescence under the uviol lamp.
Temperature Influence is investigated this product when launching for 10 ℃, 25 ℃ and 35 ℃, with the degree of separation of the corresponding spot of reference substance and other spots, the spread condition of spot and the situation of change of Rf value under the constant situation of other conditions.Test findings shows: when launching for 15 ℃, the diffusion of the initial point of need testing solution is more serious; 25 ℃ of expansion, the spot degree of separation that obtains is better, and Rf value is moderate; When launching for 35 ℃, Rf value is too high.Therefore, when launching for 25 ℃, the gained result is comparatively desirable.
The influence of expansion cylinder pre-equilibration is investigated expansion cylinder and was launched without saturated, saturated 5 minutes and saturated 10 minutes, to the influence of thin-layer chromatography under the constant situation of other conditions.Test findings shows that unsaturation is launched, and duration of run is longer, the forward position out-of-flatness; Launched in saturated 5 minutes, the degree of separation of spot is better, and Rf value is moderate; Launched in saturated 10 minutes and saturated 5 minutes, the two difference is not obvious.Therefore adopt after saturated 5 minutes and launch, the result is comparatively desirable.
The elite myricetrin of the discrimination method of Red-bayberry root herb of the present invention is a reference substance, and thin layer differentiates that test sample is handled simple, favorable reproducibility, specificity is strong.Can list it in standard, as the Red-bayberry root herb specific method.
Embodiment
Embodiment:
Get Red-bayberry root herb meal (pressing the Chinese Pharmacopoeia regulation) 2g, add ethyl acetate 30ml, sonicated (power 250W, frequency 25KHZ) 5 minutes filters, and filtrate evaporate to dryness, residue add methyl alcohol 2ml makes dissolving, as need testing solution.Other gets myricetrin (C
21H
20O
12) reference substance, add methyl alcohol and make the solution that every 1ml contains 1mg, in contrast product solution.According to thin-layered chromatography (" an appendix VI of Chinese pharmacopoeia B) test.Drawing each 2 μ l of above-mentioned two kinds of solution, put respectively on same polyamide film, is developping agent with isopropyl alcohol-water (3: 2), launch, take out, dry, spray is with 1% (W/W) aluminium choride ethanolic solution, and it is clear that hot blast blows to the spot colour developing, inspects under ultraviolet lamp (365nm).In the test sample chromatogram, with reference substance chromatogram relevant position on, show the fluorescence spot of same color.Be Red-bayberry root herb.
More than each reagent be analyze pure, myricetrin purity 〉=95%.
Claims (1)
1. the discrimination method of a Red-bayberry root herb is characterized in that with the myricetrin being reference substance, adopts thin-layered chromatography to carry out qualitative identification, it is characterized in that being made up of following steps:
One, need testing solution preparation:
Get Red-bayberry root herb meal 2g, add the ethyl acetate of 30ml, sonicated 5 minutes filters, the filtrate evaporate to dryness, and the methyl alcohol that residue adds 2ml makes dissolving;
Two, reference substance solution preparation:
Get myricetrin, add methyl alcohol and make the solution that every 1ml contains 1mg;
Three, thin-layered chromatography is differentiated
Draw each 2 μ l of above-mentioned two kinds of solution, putting respectively on same polyamide film, is developping agent with isopropyl alcohol-water 3:1-3, launches, take out, dry, spray is with 1% aluminium choride ethanolic solution, and it is clear that hot blast blows to the spot colour developing, under ultraviolet lamp, inspect, in the test sample chromatogram, with reference substance chromatogram relevant position on, show the fluorescence spot of same color.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100486650A CN100383525C (en) | 2005-12-02 | 2005-12-02 | Identifying method for Red-bayberry root herb |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100486650A CN100383525C (en) | 2005-12-02 | 2005-12-02 | Identifying method for Red-bayberry root herb |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1979160A CN1979160A (en) | 2007-06-13 |
| CN100383525C true CN100383525C (en) | 2008-04-23 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2005100486650A Active CN100383525C (en) | 2005-12-02 | 2005-12-02 | Identifying method for Red-bayberry root herb |
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| CN (1) | CN100383525C (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107167528A (en) * | 2017-04-26 | 2017-09-15 | 江南大学 | A kind of method that high flux detects naringenin |
| CN114137142B (en) * | 2021-11-25 | 2024-08-13 | 广西壮族自治区食品药品检验所 | Thin-layer chromatography identification method of Chinese sumac |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1368071A (en) * | 2001-02-05 | 2002-09-11 | 杨孟君 | Nano medicine 'Qianzihong' and its preparing process |
| CN1679795A (en) * | 2005-01-04 | 2005-10-12 | 华南理工大学 | Anti-cancer extracts from thinleaf adina root and its making method and use |
-
2005
- 2005-12-02 CN CNB2005100486650A patent/CN100383525C/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1368071A (en) * | 2001-02-05 | 2002-09-11 | 杨孟君 | Nano medicine 'Qianzihong' and its preparing process |
| CN1679795A (en) * | 2005-01-04 | 2005-10-12 | 华南理工大学 | Anti-cancer extracts from thinleaf adina root and its making method and use |
Non-Patent Citations (2)
| Title |
|---|
| 云南民间药矮杨梅根的化学成分研究(I). 文旭.中国民族民间医药杂志，第26期. 1997 * |
| 民族药杨梅跟的生药鉴定. 刘昆云等.中国民族民间医药杂志，第20期. 1996 * |
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| CN1979160A (en) | 2007-06-13 |
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