CN108956843A - A kind of quick multi information thin-layer identification method of banxia baizhu tianma decoction freeze-dried powder - Google Patents
A kind of quick multi information thin-layer identification method of banxia baizhu tianma decoction freeze-dried powder Download PDFInfo
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- CN108956843A CN108956843A CN201810838406.5A CN201810838406A CN108956843A CN 108956843 A CN108956843 A CN 108956843A CN 201810838406 A CN201810838406 A CN 201810838406A CN 108956843 A CN108956843 A CN 108956843A
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- G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography
Abstract
The present invention relates to a kind of quick multi information thin-layer identification methods of banxia baizhu tianma decoction freeze-dried powder.It is characterized by: obtaining test sample and control medicinal material solution with simple, fast pre-treating method, using same test solution, on two blocks of lamellaes, while four traditional Chinese medicine material authenticated, Radix Glycyrrhizae, Rhizoma Atractylodis Macrocephalae are on one block of lamellae, and Exocarpium Citri Rubrum, Rhizoma Gastrodiae are on another block of lamellae.After thin plate expansion, directly at ultraviolet lamp 365nm, the fluorescence spot of Radix Glycyrrhizae and Exocarpium Citri Rubrum is inspected；After spray is with the colour developing of 10% ethanol solution of sulfuric acid, at ultraviolet lamp 365nm, inspects Rhizoma Atractylodis Macrocephalae and increase the fluorescence spot after fluorescence；After spray is with the mixed solution colour developing of 3% phosphomolybdic acid ethanol solution and 10% ethanol solution of sulfuric acid to mix in equal volume, the brownish red spot of Rhizoma Gastrodiae colour generation is inspected in darkroom through light, it is overlapped, but under the conditions of respectively inspecting, it does not interfere with each other.It is controlled for the quality of banxia baizhu tianma decoction freeze-dried powder, provides simple, fast thin layer method.
Description
Technical field
The present invention relates to a kind of quick multi information thin-layer identification methods of banxia baizhu tianma decoction freeze-dried powder.
Background technique
In compound Chinese medicinal preparation, the compound preparation that especially traditional water decoction is boiled is made according to the extraction principle of similar compatibility
Fat-soluble ingredient a part is it is difficult to extract arriving in agent, and water soluble ingredient extracts more, interferes with each other serious, and water extracts
Later, identified under crude drug item with the thin layer of liposoluble constituent, some can not use, it is necessary to find the identification of water soluble ingredient
Characteristic point is limited to the difficulty of technology, so the thin layer identification that its water extracts preparation is revised and enlarged, rate is low, and quantitative determination index is few.With
The yiganning granules that the water recorded decocts only have revised and enlarged 4 by 13 taste Chinese medicinal compositions for version Chinese Pharmacopoeia one within 2015
The thin layer of taste medicinal material identifies, and it is 30% that rate is revised and enlarged in identification.Not only it is low to revise and enlarge rate for identification, and discrimination method is also very cumbersome, complicated,
The completion of 4 identifications needs sample 85g or 15g (containing lactose), only pre-treatment solvent 386ml, time 10~12 hours.4 mirror
4 kinds of test solutions are not prepared, and on 4 blocks of lamellaes, 4 expansion are completed；The Erding granules recorded are made of 4 taste medicinal materials,
It has only revised and enlarged a thin layer to identify, has revised and enlarged rate 25%.Its pre-treatment need sample 15g or 3g (containing lactose), processing solvent 82ml,
Time 2 h；The Er'bao granule recorded, by ten simply medicinal material form, revised and enlarged 4 taste medicinal materials thin layer identify, revise and enlarge rate 36%.
And discrimination method is also very cumbersome, complicated, 4 identifications will prepare 3 kinds of test solutions, on 4 blocks of lamellaes, be unfolded for 4 times
At.Sample 80g, only pre-treatment solvent 295ml, time 8 hours are needed altogether.Solvent and duration of run are such as added, cost has
Solvent and time, just more, etc., similar example is too numerous to mention.
To sum up, thin layer identification is substantially a kind, identifies if any N it is necessary to prepare N kind test solution, N block is thin
N times are unfolded in laminate, identify traditional differential mode of N taste medicinal material.For exclusive PCR, the pre-treatment program of sample is how complicated, tired
It is trivial, it need to use a large amount of organic reagent purification process repeatedly, it is laborious, time-consuming, take reagent, pollution environment, harm health, detection cycle
It is long.In this way, one identifies to detect with the quality standard of binomial assay containing 6~7 thin layers and complete, commonly take up Fei Yizhou's
Time, such as retrial, the time is double, and detection speed seriously restricts modernization of Chinese medicine speed of production.It is mentioned so finding traditional water
The simplicity of preparation is taken, rapid detection method, detection efficiency is improved, reduces testing cost, is that traditional Chinese medicine quality control must be broken through
Difficulty.
Banxia baizhu tianma decoction is the classics recipe that State Administration of Traditional Chinese Medicine announces, by prepared RHIZOMA PINELLIZE without adju-vant, Rhizoma Gastrodiae, Poria cocos, white
Art, 6 taste medicinal material of Radix Glycyrrhizae composition, in addition when water decocts, the ginger and jujube added contain altogether eight taste medicinal materials in freeze-dried powder.
Its prescription proportion and preparation process are as follows:
Prescription: each money of one Qian Wufen of the tuber of pinellia, Rhizoma Gastrodiae, Poria cocos, Exocarpium Citri Rubrum, three money of Rhizoma Atractylodis Macrocephalae, Radix Glycyrrhizae five are divided.
Preparation method: taking the Six-element medicinal material in prescription, add ginger a piece of, and two pieces of jujube, water decocts, decoction liquor concentration, and freezing is dry
It is dry, obtain freeze-dried powder.
For the quality for ensuring preparation, thin layer is carried out to banxia baizhu tianma decoction freeze-dried powder and has identified research.
Summary of the invention
The present invention identifies if any N aiming at the previous kind of mesh it is necessary to prepare N kind test solution, N block thin layer
N times are unfolded in plate, identify traditional differential mode of N taste medicinal material.Pass through the various combination of solvent and its ratio, color developing agent, thin layer
The parameter studies such as carrier and testing conditions are created using same test solution, and on two blocks of lamellaes, 4 kinds different
Under the conditions of inspecting, the survey for identifying 4 taste medicinal materials comments thin layer to identify new model more.Radix Glycyrrhizae, Rhizoma Atractylodis Macrocephalae are on another block of lamellae, tangerine
Red, Rhizoma Gastrodiae is on one block of lamellae.Rhizoma Atractylodis Macrocephalae water extracts the increasing fluorescence detection of preparation, the developing technology of Rhizoma Gastrodiae is to report for the first time.
The present invention solves scheme used by its technical problem are as follows:
A. banxia baizhu tianma decoction freeze-dried powder 0.5g is taken, methanol 2ml is added, is ultrasonically treated 10 minutes, centrifugation, supernatant conduct
Test solution；Rhizoma Atractylodis Macrocephalae control medicinal material 0.2g separately is taken, adds methanol 2ml, is ultrasonically treated 20 minutes, supernatant is as Rhizoma Atractylodis Macrocephalae comparison medicine
Material solution；Extracting liquorice control medicinal material 0.1g again adds water 30ml, and small fire decocts 20 minutes, and filtration, filtrate is concentrated to dryness, and residue adds
Methanol 2ml makes to dissolve, as Radix Glycyrrhizae control medicinal material solution；It is molten to draw 2~3 μ l of Radix Glycyrrhizae control medicinal material solution, Rhizoma Atractylodis Macrocephalae control medicinal material
6~8 μ l of liquid, 8~10 μ l of test solution, put respectively in same silica G F254On lamellae, with volume ratio 13: 2: 3: 0.5
The dense ammonia of chloroform-acetate-methanol-is solvent, is unfolded, and is taken out, and hot blast drying is set and inspected under ultraviolet lamp 365nm,
In sample chromatogram, on position corresponding with Radix Glycyrrhizae reference medicine chromatography, same color fluorescence principal spot (Fig. 1) is shown；It sprays again
With 10% ethanol solution of sulfuric acid, 105 DEG C to be heated to spot development clear, sets and inspects under ultraviolet lamp 365nm, sample chromatogram
In, on position corresponding with Rhizoma Atractylodis Macrocephalae reference medicine chromatography, show same color fluorescence principal spot (Fig. 2)；
B. Rhizoma Gastrodiae control medicinal material and each 0.2g of Exocarpium Citri Rubrum control medicinal material are taken, respectively plus methanol 2ml, is ultrasonically treated 20 minutes, on
Clear liquid is as respective control medicinal material solution；Separately take Gastrodin and aurantiamarin reference substance appropriate, respectively plus methanol is made every 1ml and contains
The solution of 1mg, as respective reference substance solution；Draw 3~4 μ l of Gastrodin reference substance solution, 5~6 μ of aurantiamarin reference substance solution
L, each 5~6 μ l of control medicinal material solution, 6~8 μ l of test solution under A, put respectively in same silica G F254On lamellae,
Using ethyl acetate-butanone-methyl alcohol-formic acid-water of volume ratio 8: 3: 1: 0.2: 0.2 as solvent, exhibition to about 3cm is taken out, hot wind
Drying, then set exhibition in same solvent and taken out, hot blast drying is set and inspected under ultraviolet lamp 365nm, sample chromatogram to 10cm
In, on position corresponding with Exocarpium Citri Rubrum reference medicine chromatography, show identical bright blue fluorescence principal spot (Fig. 3)；Set ultraviolet lamp
It is inspected under 254nm, in sample chromatogram, on position corresponding with aurantiamarin reference substance and Exocarpium Citri Rubrum reference medicine chromatography, respectively
The principal spot (Fig. 4) of aobvious same color；3% phosphomolybdic acid ethanol solution and 10% sulfuric acid ethyl alcohol mixed with volume ratio 1: 1 is sprayed again
The mixed solution of solution, 105 DEG C are heated to clear spot, set in darkroom, inspect through light, in sample chromatogram, with day
On numb element and the corresponding position of Rhizoma Gastrodiae reference medicine chromatography, same color principal spot (Fig. 5) is shown respectively.
The principle of the present invention is as follows:
Chemical structure and property according to each effective component of Chinese medicine, it then follows the extraction principle of similar compatibility, using suitable
Extraction solvent, simplicity quickly prepare test sample and control medicinal material solution.Again with different component, different proportion, opposed polarity
Solvent is unfolded, and various chemical components will be with different solvents, and foundation Adsorption and desorption is attached, adsorbs, solves again again
The ability of absorption is different, and is able to good separation on respective lamellae.Again by the approximate effective component of various polarity,
On same lamellae, it is different inspect under the conditions of, although overlapping, by different colour developing means, makes it in different layers
It on secondary, does not interfere with each other, shows respectively different colored speckles, obtain the thin-layer chromatogram of multi information.Realize it is easy, quick,
Inexpensive, efficient thin layer hope.
It innovative point of the invention and has the beneficial effect that:
1. using the dense ammonia of chloroform-acetate-methanol-of volume ratio 13: 2: 3: 0.5 as solvent, expansion Radix Glycyrrhizae and
Rhizoma Atractylodis Macrocephalae inspects the fluorescence principal spot of Radix Glycyrrhizae first at ultraviolet lamp 365nm, and Rhizoma Atractylodis Macrocephalae is without any information spot；Spray with
10% ethanol solution of sulfuric acid, 105 DEG C to be heated to spot development clear, sets and is inspected after Rhizoma Atractylodis Macrocephalae increases fluorescence under ultraviolet lamp 365nm
Fluorescence principal spot, clear spot is vivid, easily inspects very much.What its innovation was that 1) Rhizoma Atractylodis Macrocephalae constant testing be all it is fat-soluble at
The colored speckles point to develop the color with vanillin-sulfuric acid solution, sensitivity is not high, yet there are no after inspecting and developing the color with ethanol solution of sulfuric acid
Water soluble ingredient highly sensitive fluorescence spot；2) this solvent is alkaline, can not only be made very big by one in compound preparation
Partially acidic ingredient slows down the attached ability of its Adsorption and desorption at salt, reduces the interference of thin layer background, makes by acid ingredient covering
Rhizoma Atractylodis Macrocephalae spot releases covering, moreover it is possible to the fluorescence intensity of the Rhizoma Atractylodis Macrocephalae fluorescence spot after enhancing colour developing；3) it solves so far, Rhizoma Atractylodis Macrocephalae
After water decocts, liposoluble constituent is lost, and finds the problem less than thin layer authentication information spot；4) what this identification was inspected is white
The water-soluble maximum fluorescence spot of art, and the solvent just all shifts some fat-soluble phosphor dots of control medicinal material onto
The forward position of solvent, solving Rhizoma Atractylodis Macrocephalae in preparation is mainly water soluble ingredient, and Rhizoma Atractylodis Macrocephalae control medicinal material liposoluble constituent content is high,
Cause the two different cause of spot, and problem can not be described.5) the Rhizoma Atractylodis Macrocephalae detection in compound preparation is decocted for water, provides row
Effective discrimination method, have innovation and practicality.
2. Rhizoma Gastrodiae is unfolded using ethyl acetate-butanone-methyl alcohol-formic acid-water of volume ratio 8: 3: 1: 0.2: 0.2 as solvent
And Exocarpium Citri Rubrum, it is first inspected at ultraviolet lamp 365nm, 3 fluorescence principal spots of Exocarpium Citri Rubrum, then inspect Exocarpium Citri Rubrum at ultraviolet lamp 254nm
With the sepia spot of aurantiamarin, aurantiamarin and sapphirine spot are two kinds of chemical components (Fig. 6) being located next to.Spray again with etc.
3% phosphomolybdic acid ethanol solution of volume mixture and the mixed solution of 10% ethanol solution of sulfuric acid, 105 DEG C are baked to clear spot,
It sets in darkroom, the brownish red principal spot of Gastrodin and Rhizoma Gastrodiae control medicinal material is inspected through light；This identification of comprehensive analysis, it is believed that:
It is the sepia spot of aurantiamarin below bright blue fluorescence spot, Chong Die with bright fluorescence spot blue there are also the days after developing the color
Numb element spot, although they are overlapped.But an observation fluorescence spot, an observation sepia spot, after an observation colour developing
Spot has been accomplished not interfereing with each other, to report for the first time.
3. the color developing agent so far, identified in relation to Rhizoma Gastrodiae thin layer is all 10% phosphomolybdic acid ethanol solution, it is one
The color developing agent of wide scope, all reacts with most of ingredient, all shows the spot of blue, it is difficult to the identification feature spot from color
Point.Moreover, and a kind of solution that 10% phosphomolybdic acid ethanol solution is blue has color back to the lamellae after spraying
Scape interference, and color speed is very slow, it is necessary to easily there is the situation not in place that develops the color very much in long-time heating, influences identification
Accuracy judgement.And the application is using 3% phosphomolybdic acid ethanol solution and 10% ethanol solution of sulfuric acid to mix in equal volume
Mixed solution, be not only obviously improved the color speed of Rhizoma Gastrodiae, also made the color of the effective component Gastrodin spot of Rhizoma Gastrodiae, by
Blue becomes brownish red, hence it is evident that distinguishes with the blue spot of aurantiamarin, facilitates promotion testing staff and sentence to thin layer spot
Cutting capacity.
4. the mixed solution being made of isometric 3% phosphomolybdic acid ethanol solution and 10% ethanol solution of sulfuric acid, as thin
Layer identifies color developing agent, and not only color speed is fast, and can also make the different effective components of Chinese medicine, generates different colours, yet there are no
Report identifies field for thin layer and provides a kind of new color developing agent, has innovative and popularization and application foreground.
5. the identification is completed compared with traditional thin layer for enumerating under background technique item identifies, only need sample 0.5g, Rhizoma Atractylodis Macrocephalae,
Exocarpium Citri Rubrum, each 0.2g of Rhizoma Gastrodiae control medicinal material, Radix Glycyrrhizae control medicinal material 0.1g, organic process solvent 11ml, solvent 30ml, time 1 are small
When.Method is easy, quick, efficient, and as many as detection information, report method does not have at present.For Banxia Baizhu day
The quality surveillance of numb soup freeze-dried powder, provides effective quality control method.Embody detection technique innovation necessity and
The significant beneficial effect of bring.
Detailed description of the invention
Fig. 1 is the Radix Glycyrrhizae TLC figure that banxia baizhu tianma decoction freeze-dried powder is inspected at ultraviolet lamp 365nm.
Fig. 2 is after 10% ethanol solution of sulfuric acid of banxia baizhu tianma decoction freeze-dried powder develops the color, to inspect at ultraviolet lamp 365nm
Rhizoma Atractylodis Macrocephalae TLC figure.
Fig. 3 is the Exocarpium Citri Rubrum TLC figure that banxia baizhu tianma decoction freeze-dried powder is inspected at ultraviolet lamp 365nm
Fig. 4 is the Exocarpium Citri Rubrum that banxia baizhu tianma decoction freeze-dried powder is inspected at ultraviolet lamp 254nm and aurantiamarin TLC figure
Fig. 5 is 3% phosphomolybdic acid ethanol solution and 10% of the banxia baizhu tianma decoction freeze-dried powder spray to be mixed with volume ratio 1: 1
After the mixed solution colour developing of ethanol solution of sulfuric acid, the Rhizoma Gastrodiae and Gastrodin TLC inspected through light are schemed
Fig. 6 is TLC figure of the banxia baizhu tianma decoction freeze-dried powder at double light source ultraviolet lamp 365nm and 254nm
Fig. 1,2 be same lamellae, difference inspect under the conditions of thin-layer chromatogram, wherein 1. Rhizoma Atractylodis Macrocephalae blank, 2. Rhizoma Atractylodis Macrocephalae
6. Radix Glycyrrhizae blank of control medicinal material 3.4.5 sample, 7. Radix Glycyrrhizae control medicinal material
Fig. 3,4 be same lamellae, difference inspect under the conditions of thin-layer chromatogram, wherein 1. Gastrodin, 2. Rhizoma Gastrodiae pair
According to 6. Exocarpium Citri Rubrum control medicinal material of medicinal material 3.4.5 sample, 7. aurantiamarin reference substance
4. Exocarpium Citri Rubrum control medicinal material of 1.2.3 sample, 5. aurantiamarin in Fig. 6
The specific embodiment of the invention is as follows:
A. banxia baizhu tianma decoction freeze-dried powder 0.5g is taken, methanol 2ml is added, is ultrasonically treated 10 minutes, centrifugation, supernatant conduct
Test solution；Rhizoma Atractylodis Macrocephalae control medicinal material 0.2g separately is taken, adds methanol 2ml, is ultrasonically treated 20 minutes, supernatant is as Rhizoma Atractylodis Macrocephalae comparison medicine
Material solution；Extracting liquorice control medicinal material 0.1g again adds water 30ml, and small fire decocts 20 minutes, and filtration, filtrate is concentrated to dryness, and residue adds
Methanol 2ml makes to dissolve, as Radix Glycyrrhizae control medicinal material solution；It is molten to draw 2~3 μ l of Radix Glycyrrhizae control medicinal material solution, Rhizoma Atractylodis Macrocephalae control medicinal material
6~8 μ l of liquid, 8~10 μ l of test solution, put respectively in same silica G F254On lamellae, with volume ratio 13: 2: 3: 0.5
The dense ammonia of chloroform-acetate-methanol-is solvent, is unfolded, and is taken out, and hot blast drying is set and inspected under ultraviolet lamp 365nm,
In sample chromatogram, on position corresponding with Radix Glycyrrhizae reference medicine chromatography, same color fluorescence principal spot is shown；It is sprayed again with 10%
Ethanol solution of sulfuric acid, 105 DEG C to be heated to spot development clear, sets and inspects under ultraviolet lamp 365nm, in sample chromatogram, with
On the corresponding position of Rhizoma Atractylodis Macrocephalae reference medicine chromatography, same color fluorescence principal spot is shown；
B. Rhizoma Gastrodiae control medicinal material and each 0.2g of Exocarpium Citri Rubrum control medicinal material are taken, respectively plus methanol 2ml, is ultrasonically treated 20 minutes, on
Clear liquid is as respective control medicinal material solution；Separately take Gastrodin and aurantiamarin reference substance appropriate, respectively plus methanol is made every 1ml and contains
The solution of 1mg, as respective reference substance solution；Draw 3~4 μ l of Gastrodin reference substance solution, 5~6 μ of aurantiamarin reference substance solution
L, each 5~6 μ l of control medicinal material solution, 6~8 μ l of test solution under A, put respectively in same silica G F254On lamellae,
Using ethyl acetate-butanone-methyl alcohol-formic acid-water of volume ratio 8: 3: 1: 0.2: 0.2 as solvent, exhibition to about 3cm is taken out, hot wind
Drying, then set exhibition in same solvent and taken out, hot blast drying is set and inspected under ultraviolet lamp 365nm, sample chromatogram to 10cm
In, on position corresponding with Exocarpium Citri Rubrum reference medicine chromatography, show identical bright blue fluorescence principal spot；Set ultraviolet lamp 254nm
Under inspect, in sample chromatogram, on position corresponding with aurantiamarin reference substance and Exocarpium Citri Rubrum reference medicine chromatography, show respectively identical
The principal spot of color；The mixing for spraying 3% phosphomolybdic acid ethanol solution and 10% ethanol solution of sulfuric acid that mix with volume ratio 1: 1 again is molten
Liquid, 105 DEG C are heated to clear spot, set in darkroom, inspect through light, in sample chromatogram, with Gastrodin and Rhizoma Gastrodiae pair
According on the corresponding position of medicinal material chromatography, same color principal spot is shown respectively.
Claims (2)
1. a kind of quick multi information thin-layer identification method of banxia baizhu tianma decoction freeze-dried powder, it is characterised in that:
A. banxia baizhu tianma decoction freeze-dried powder 0.5g is taken, methanol 2ml is added, is ultrasonically treated 10 minutes, centrifugation, supernatant is used as examination
Product solution；Rhizoma Atractylodis Macrocephalae control medicinal material 0.2g separately is taken, adds methanol 2ml, is ultrasonically treated 20 minutes, supernatant is molten as Rhizoma Atractylodis Macrocephalae control medicinal material
Liquid；Extracting liquorice control medicinal material 0.1g again adds water 30ml, and small fire decocts 20 minutes, and filtration, filtrate is concentrated to dryness, and residue adds methanol
2ml makes to dissolve, as Radix Glycyrrhizae control medicinal material solution；Absorption 2~3 μ l of Radix Glycyrrhizae control medicinal material solution, Rhizoma Atractylodis Macrocephalae control medicinal material solution 6~
8 μ l, 8~10 μ l of test solution, put respectively in same silica G F254On lamellae, with three chloromethanes of volume ratio 13: 2: 3: 0.5
The dense ammonia of alkane-acetate-methanol-is solvent, is unfolded, and is taken out, and hot blast drying is set and inspected under ultraviolet lamp 365nm, test sample
In chromatography, on position corresponding with Radix Glycyrrhizae reference medicine chromatography, same color fluorescence principal spot is shown；It is sprayed again with 10% sulfuric acid second
Alcoholic solution, 105 DEG C to be heated to spot development clear, sets and inspects under ultraviolet lamp 365nm, in sample chromatogram, with Rhizoma Atractylodis Macrocephalae pair
According on the corresponding position of medicinal material chromatography, same color fluorescence principal spot is shown；
B. Rhizoma Gastrodiae control medicinal material and each 0.2g of Exocarpium Citri Rubrum control medicinal material are taken, respectively plus methanol 2ml, is ultrasonically treated 20 minutes, supernatant
As respective control medicinal material solution；Separately take Gastrodin and aurantiamarin reference substance appropriate, respectively plus methanol is made every 1ml and contains 1mg
Solution, as respective reference substance solution；Draw 3~4 μ l of Gastrodin reference substance solution, 5~6 μ l of aurantiamarin reference substance solution,
Each 5~6 μ l of control medicinal material solution, 6~8 μ l of test solution under A, put respectively in same silica G F254On lamellae, with
Ethyl acetate-butanone-methyl alcohol-formic acid-water of volume ratio 8: 3: 1: 0.2: 0.2 is solvent, and exhibition to about 3cm is taken out, and hot wind is blown
It is dry, then set and opened up in same solvent to 10cm, it taking out, hot blast drying is set and is inspected under ultraviolet lamp 365nm, in sample chromatogram,
On position corresponding with Exocarpium Citri Rubrum reference medicine chromatography, identical bright blue fluorescence principal spot is shown；It sets and is examined under ultraviolet lamp 254nm
Depending on position corresponding with aurantiamarin reference substance and Exocarpium Citri Rubrum reference medicine chromatography, showing same color respectively in sample chromatogram
Principal spot；The mixed solution of 3% phosphomolybdic acid ethanol solution and 10% ethanol solution of sulfuric acid that mix with volume ratio 1: 1 is sprayed again,
105 DEG C are heated to clear spot, set in darkroom, inspect through light, in sample chromatogram, with Gastrodin and Rhizoma Gastrodiae comparison medicine
Wood color is composed on corresponding position, shows same color principal spot respectively.
2. the quick multi information thin-layer identification method of a kind of banxia baizhu tianma decoction freeze-dried powder according to claim 1, special
Sign is the suitable 2.4~2.8g of primary crude drug of the every 1g of freeze-dried powder.
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| CN201810838406.5A CN108956843B (en) | 2018-07-27 | 2018-07-27 | Rapid multi-information thin-layer identification method for pinellia ternate, bighead atractylodes rhizome and gastrodia elata decoction freeze-dried powder |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110031588A (en) * | 2019-03-26 | 2019-07-19 | 浙江金大康动物保健品有限公司 | An a kind of quick thin-layer identification method of plate multiple medicine taste of livestock and poultry antiviral granule |
| CN114689783A (en) * | 2020-12-31 | 2022-07-01 | 四川新绿色药业科技发展有限公司 | Rapid thin-layer identification method for poria cocos, cassia twig, rhizoma atractylodis and licorice root decoction freeze-dried powder |
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| CN114689783B (en) * | 2020-12-31 | 2023-09-12 | 四川新绿色药业科技发展有限公司 | Quick thin-layer identification method for poria, cassia, rhizoma atractylodis and sweet soup freeze-dried powder |
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