CN104991031A - Rapid multiple information thin-layer identification method of smilax glabra and water extract thereof - Google Patents
Rapid multiple information thin-layer identification method of smilax glabra and water extract thereof Download PDFInfo
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Abstract
The invention relates to a rapid multiple information thin-layer identification method of smilax glabra and water extract thereof. The method is characterized in that a convenient and rapid pretreatment method is used to prepare a sample and a reference solution, then on a same thin-layer plate, the multiple information thin-layer identification graph of smilax glabra and water extract thereof is examined under three different examination conditions, and the spots are clear, intersect with each other, and do not interfere with each other at the same time. The multiple information thin-layer chromatogram under different examination conditions improves the quality controllability of smilax glabra and water extract thereof, and at the same time provides multiple information choices for removing the interference, so the provided method can be applied to identify smilax glabra in a compound preparation and has the advantages of low cost and high efficiency. Furthermore, the provided method has the advantages of convenience, rapidness, efficiency, low cost, and no environmental pollution.
Description
Technical field
The present invention relates to the quick multi information thin-layer identification method of a kind of rhizoma smilacis glabrae medicinal material and water extract thereof.
Background technology
There is thin-layer identification method under the medicinal material item that Chinese medicine smilax records in Chinese Pharmacopoeia version in 2010 at present, but in its compound preparation and water extract, also it is not carried out to the report of TLC distinguish, By consulting literatures
[1,2]although there is the report of TLC distinguish in the formulation, but the pre-treatment of sample and control medicinal material all compares very complicated, need by organic solvent purification process repeatedly, only the pre-treatment of sample will spend a few hours, the organic reagent of hundreds of ml, efficiency is low, environmental pollution is serious, endanger the healthy of operating personnel.Now find to obtain smilax thin-layer identification method, enumerate and be analyzed as follows:
The thin-layer identification method of Chinese Pharmacopoeia version in 2010 rhizoma smilacis glabrae medicinal material gets this product powder 1g, adds methyl alcohol 20ml, ultrasonic process 30 minutes, and filter, filtrate is as need testing solution.Separately get astilbin reference substance, add methyl alcohol and make the solution of every 1ml containing 0.1mg, product solution in contrast.Test according to thin-layered chromatography (Chinese Pharmacopoeia version in 2010 annex VIB), draw each 10 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate, with toluene-ethyl acetate-formic acid (13: 32: 9) for developping agent, launch, take out, dry, spray, with aluminium choride test solution, is placed after 5 minutes, is inspected under putting ultraviolet lamp (365nm).In test sample chromatogram, on the position corresponding to reference substance chromatogram, the spot of aobvious same color.
This discrimination method adopts astilbin reference substance to be index, carry out smilax TLC distinguish, although sample pre-treatments is uncomplicated, but quantity of information is single, only inspect spraying aluminium choride test solution, place the fluorescence spot after 5 minutes, differentiate a batch sample, sample pre-treatments spends 0.5 hour, organic solvent 20ml.
Document
[1]in the Jieduxiezhu I granules of report, the thin-layer identification method of smilax gets Jieduxiezhu I granules in right amount, and porphyrize, takes 2g, adds methyl alcohol 50ml, ultrasonic process 30 minutes, and filter, filtrate evaporate to dryness, residue adds 3molL
-1hydrochloric acid 20ml, gradation is dissolved, and is transferred in conical flask, and heating water bath is hydrolyzed 30 minutes, and taking-up lets cool, and adds chloroform 60ml, and point three extractions, combined chloroform layer, filter, evaporate to dryness, residue adds methyl alcohol 2ml makes dissolving, as need testing solution.Separately get diosgenin reference substance appropriate, add methyl alcohol and make the solution of every 1ml containing 0.2mg, product solution in contrast.Test according to thin-layered chromatography (Chinese Pharmacopoeia version in 2010 annex VIB), draw need testing solution 10 μ l, reference substance solution 5 μ l, put respectively on same silica gel g thin-layer plate, with methenyl choloride-methyl alcohol (20: 0.2) for developping agent, presaturation, after 20 minutes, launches, take out, dry, spray with 10% phosphomolybdic acid ethanol solution, be heated to spot development at 105 DEG C clear.In test sample chromatogram, on the position corresponding to reference substance chromatogram, the spot of aobvious same color.
This discrimination method adopts diosgenin to be index, carries out smilax TLC distinguish, differentiate a batch sample, only sample pre-treatments want spended time 3 hours, organic solvent 230ml.
Document
[2]in the Yinxie capsule of report, the TLC distinguish of smilax gets Yinxie capsule content 3g, and add water 60ml, ultrasonic process 30 minutes, let cool, be transferred in separating funnel, add hydrochloric acid and adjust pH to 2, then add ethyl acetate and jolt extraction 2 times, each 20ml, merge extract, by after the funnel that is covered with anhydrous sodium sulfate, by filtrate evaporate to dryness, adding methyl alcohol 1ml makes residue dissolve, and lysate is as need testing solution.Separately fetch earth Poria cocos control medicinal material 5g, and add water 100ml, adds hot reflux 4 hours, and filter, filtrate is concentrated into about 10ml, adds ethanol 15ml, stirs, and leaves standstill, and filter, filtrate evaporate to dryness, by the preparation method of need testing solution, prepares control medicinal material solution.Test according to thin-layered chromatography (Chinese Pharmacopoeia version in 2010 annex VIB), draw need testing solution and each 10 μ l of control medicinal material solution, put respectively on same silica gel g thin-layer plate, with methenyl choloride-methyl alcohol-formic acid (15: 5: 1) for developping agent, launch, take out, dry, spray with ferric trichloride ethanolic solution, in test sample chromatogram, on the position corresponding to control medicinal material chromatogram, the spot of aobvious same color.
This discrimination method is with smilax control medicinal material for contrast TLC distinguish, and differentiate a batch sample, only spended time 7-8 hour, organic solvent 97ml are wanted in the pre-treatment of sample and control medicinal material.
By above-mentioned smilax TLC distinguish example in the formulation, describe so far, the unicity of its triviality differentiated, complicacy, time-consuming, quantity of information, to the contaminative of surrounding environment and high cost, poor efficiency.So improve detection efficiency, reduction testing cost, minimizing environmental pollution, create the quick multi information thin-layer identification method of rhizoma smilacis glabrae medicinal material and water extract thereof, become the instant objective of the struggle of testing staff, we are exactly under this background condition, have invented the quick multi information thin-layer identification method of rhizoma smilacis glabrae medicinal material and water extract thereof.
Preparation method's smilax medicine materical crude slice 7000-8000g of rhizoma smilacis glabrae medicinal material water extract or granule, add water 8 times amount respectively, decocts and extract secondary, each 1-2 hour, filters, filtrate 70 DEG C of reduced pressure concentrations, concentrate spraying dry or 70 DEG C of drying under reduced pressure, obtain smilax water extract dried cream powder; Or it is appropriate to add dextrin, mixing, granulates, makes 1000g respectively, packing, obtain smilax water extraction and get granule.
Summary of the invention
The present invention does not also have thin-layer identification method for smilax water extract and its TLC distinguish in compound preparation is loaded down with trivial details, time-consuming, quantity of information is single, the drawback of method contaminated environment and high cost, has invented the quick multi information thin-layer identification method of a kind of rhizoma smilacis glabrae medicinal material and water extract thereof.Namely, test sample and control medicinal material solution is obtained with the most simple, fast pre-treating method, on same thin layer plate, obtain smilax and its water extract at ultraviolet lamp 365nm and 254nm, and after spraying 10% ethanol solution of sulfuric acid develops the color, daylight, inspect the multi information TLC distinguish figure under condition for 3 kinds, clear spot, interlaced, but do not interfere with each other (see Fig. 1,2,3).Compared with single information thin layer of routine, not only increase substantially the quality controllability of rhizoma smilacis glabrae medicinal material and extract thereof, be conducive to quality supervision, but also improve detection efficiency, saved cost, decrease environmental pollution.And be the quick TLC distinguish of smilax in the compound preparation that extracts, provide three kinds of thin-layer chromatograms inspected under condition and select reference, in order to exclusive PCR composition.
The present invention solves the scheme that its technical matters adopts:
(1) to get rhizoma smilacis glabrae medicinal material and its control medicinal material appropriate for the TLC distinguish of rhizoma smilacis glabrae medicinal material, and porphyrize, takes 0.3g respectively, and add each 2ml of 70% methyl alcohol, ultrasonic process 20 minutes, supernatant is respectively as need testing solution and control medicinal material solution; Draw above-mentioned control medicinal material solution and each 5 ~ 8 μ l of need testing solution, put respectively in same silica G F
254on thin layer plate, with volume ratio be the methenyl choloride-acetate-methanol-formic acid of 8: 2: 3: 0.3 for developping agent, launch, take out, hot blast drying, inspects under putting ultraviolet lamp 365nm, in test sample chromatogram, on the position corresponding to control medicinal material chromatogram, aobvious same color fluorescence principal spot; Inspect under putting ultraviolet lamp 254nm, in test sample chromatogram, on the position corresponding to control medicinal material chromatogram, aobvious same color principal spot; Spray with 10% ethanol solution of sulfuric acid again, 105 DEG C to be heated to spot development clear, in test sample chromatogram, on the position corresponding to control medicinal material chromatogram, and the principal spot of aobvious same color.
(2) TLC distinguish of smilax water extract gets this product in right amount, and porphyrize, takes 0.2g, adds 70% methyl alcohol 5ml, ultrasonic process 10 minutes, and filter, filtrate is as need testing solution.Separately fetch earth Poria cocos control medicinal material 0.3g, adds 70% methyl alcohol 2ml, ultrasonic process 20 minutes, gets supernatant medicinal material solution in contrast.According to thin-layered chromatography (annex VIB) test, draw above-mentioned control medicinal material solution and each 5 ~ 8 μ l of need testing solution, put respectively in same silica G F
254on thin layer plate, with volume ratio be the methenyl choloride-acetate-methanol-formic acid of 8: 2: 3: 0.3 for developping agent, launch, take out, hot blast drying, inspects under putting ultraviolet lamp 365nm, in test sample chromatogram, on the position corresponding to control medicinal material chromatogram, aobvious same color fluorescence principal spot; Inspect under putting ultraviolet lamp 254nm, in test sample chromatogram, on the position corresponding to control medicinal material chromatogram, aobvious same color principal spot; Spray with 10% ethanol solution of sulfuric acid again, 105 DEG C to be heated to spot development clear, in test sample chromatogram, on the position corresponding to control medicinal material chromatogram, and the principal spot of aobvious same color.
Principle of the present invention is as follows:
According to chemical constitution and the character of Chinese medicine each effective constituent, follow the extraction principle of similar compatibility, adopt suitable Extraction solvent, easy, prepare test sample and control medicinal material solution quickly.Again by the movement of developping agent, the difference of the attached ability of the Adsorption and desorption of each composition on thin layer plate, makes its spot be separated.Again by each compositional polarity size, choose the effective constituent that polarity is approximate, under different conditions of inspecting, present different spot colors, on same thin layer plate, different inspect condition under, obtain the thin-layer chromatogram of multi information.
Innovative point of the present invention and beneficial effect as follows:
1. the pre-treating method of rhizoma smilacis glabrae medicinal material and control medicinal material is: take 0.3g rhizoma smilacis glabrae medicinal material and its control medicinal material respectively, add methyl alcohol 2ml, and after ultrasonic process, supernatant is directly as test sample and control medicinal material solution; Easy, quick, only about 20 minutes pre-treatment time of cost, organic solvent 4ml.Compared with official method, although only save 10 minutes processing times, Extraction solvent 16ml, quantity of information is three times of report method.
2. the pre-treating method of smilax water extract also: take a morsel smilax water extract and control medicinal material, adds a small amount of 70% methyl alcohol, and after ultrasonic process, filtrate and supernatant are directly as test sample and control medicinal material solution; Spend 20 minutes pre-treatment time, organic solvent 7ml altogether.Compared with report method, often detect a collection of medicinal material, save time 2.5-7 hour, organic solvent 90-223ml.
3. the quantity of information detected is 3 colored speckles after the bright blue look fluorescence principal spot under ultraviolet lamp (365nm), 2 fluorescence quenching spots under ultraviolet lamp (254nm) and colour developing under daylight.Three kinds of differences inspect the multi information collection of illustrative plates under condition, and the condition of inspecting when providing exclusive PCR composition in compound preparation is selected.
4. this discrimination method is also with the difference of report method: the developping agent of this discriminating is by methenyl choloride, ethyl acetate, methyl alcohol, the organic solvent composition of formic acid 4 kinds of opposed polarities, report method is made up of the organic solvent of 2 kinds or 3 kinds opposed polarities, consider from the separating resulting of similar compatibility, the separating effect of developping agent and the kind of solvent are proportionate, but the difficulty obtaining best developping agent component proportion is also proportionate with the kind of solvent, so through the component of multiple developping agent and ratio discuss and study, obtain developping agent best of breed, obtain three kinds and inspect multi information collection of illustrative plates under condition, and the separating effect of each information spot is all more satisfactory.
5. smilax water extract is compared with the thin-layer identification method of smilax in the preparation of report, and detection efficiency is high, and testing cost is low, non-environmental-pollution, for the quick TLC distinguish extracting smilax in compound preparation provides three kinds of collection of illustrative plates references of inspecting under condition.
Accompanying drawing explanation
Fig. 1 is that rhizoma smilacis glabrae medicinal material and the thin layer TLC of extract under ultraviolet lamp 365nm thereof scheme.
Fig. 2 is that rhizoma smilacis glabrae medicinal material and the thin layer TLC of extract under ultraviolet lamp 254nm thereof scheme.
Fig. 3 is that after rhizoma smilacis glabrae medicinal material and extract thereof develop the color, the thin layer TLC under daylight schemes.
Fig. 1,2,3 is same thin layer plate, and difference inspects the thin-layer chromatogram under condition, and wherein, 1.2.3 is smilax water extract; 5.6.7 be rhizoma smilacis glabrae medicinal material; 4. be smilax control medicinal material
The specific embodiment of the invention is as follows:
(1) to get rhizoma smilacis glabrae medicinal material and its control medicinal material appropriate for the TLC distinguish of rhizoma smilacis glabrae medicinal material, and porphyrize, takes 0.3g respectively, and add each 2ml of 70% methyl alcohol, ultrasonic process 20 minutes, supernatant is respectively as need testing solution and control medicinal material solution; Draw above-mentioned control medicinal material solution and each 5 ~ 8 μ l of need testing solution, put respectively in same silica G F
254on thin layer plate, with volume ratio be the methenyl choloride-acetate-methanol-formic acid of 8: 2: 3: 0.3 for developping agent, launch, take out, hot blast drying, inspects under putting ultraviolet lamp 365nm, in test sample chromatogram, on the position corresponding to control medicinal material chromatogram, aobvious same color fluorescence principal spot; Inspect under putting ultraviolet lamp 254nm, in test sample chromatogram, on the position corresponding to control medicinal material chromatogram, aobvious same color principal spot; Spray with 10% ethanol solution of sulfuric acid again, 105 DEG C to be heated to spot development clear, in test sample chromatogram, on the position corresponding to control medicinal material chromatogram, and the principal spot of aobvious same color;
(2) TLC distinguish of smilax water extract gets this product in right amount, and porphyrize, takes 0.2g, adds 70% methyl alcohol 5ml, ultrasonic process 10 minutes, and filter, filtrate is as need testing solution.Separately fetch earth Poria cocos control medicinal material 0.3g, adds 70% methyl alcohol 2ml, ultrasonic process 20 minutes, gets supernatant medicinal material solution in contrast.According to thin-layered chromatography (annex VIB) test, draw above-mentioned control medicinal material solution and each 5 ~ 8 μ l of need testing solution, put respectively in same silica G F
254on thin layer plate, with volume ratio be the methenyl choloride-acetate-methanol-formic acid of 8: 2: 3: 0.3 for developping agent, launch, take out, hot blast drying, inspects under putting ultraviolet lamp 365nm, in test sample chromatogram, on the position corresponding to control medicinal material chromatogram, aobvious same color fluorescence principal spot; Inspect under putting ultraviolet lamp 254nm, in test sample chromatogram, on the position corresponding to control medicinal material chromatogram, aobvious same color principal spot; Spray with 10% ethanol solution of sulfuric acid again, 105 DEG C to be heated to spot development clear, in test sample chromatogram, on the position corresponding to control medicinal material chromatogram, and the principal spot of aobvious same color.
List of references
[1] Zhang Mingsheng, Gao Jiarong, Wei Liangbing " the indentification by TLC research of Jieduxiezhu I granules " Anhui medicine, 2007,11 (4) 350-351
[2] Chen Xiuyuan, Li Hongyan, Zhao Hua " the thin-layer chromatography Qualitive test of Yinxie capsule " Chinese Medicine science, 2013,3 (1) 111-112
Claims (3)
1. the present invention relates to the quick multi information thin-layer identification method of a kind of rhizoma smilacis glabrae medicinal material and water extract thereof, it is characterized in that:
(1) to get rhizoma smilacis glabrae medicinal material and its control medicinal material appropriate for the TLC distinguish of rhizoma smilacis glabrae medicinal material, and porphyrize, takes 0.3g respectively, and add each 2ml of 70% methyl alcohol, ultrasonic process 20 minutes, supernatant is respectively as need testing solution and control medicinal material solution; Draw above-mentioned control medicinal material solution and each 5 ~ 8 μ l of need testing solution, put respectively in same silica G F
254on thin layer plate, with volume ratio be the methenyl choloride-acetate-methanol-formic acid of 8: 2: 3: 0.3 for developping agent, launch, take out, hot blast drying, inspects under putting ultraviolet lamp 365nm, in test sample chromatogram, on the position corresponding to control medicinal material chromatogram, aobvious same color fluorescence principal spot; Inspect under putting ultraviolet lamp 254nm, in test sample chromatogram, on the position corresponding to control medicinal material chromatogram, aobvious same color principal spot; Spray with 10% ethanol solution of sulfuric acid again, 105 DEG C to be heated to spot development clear, in test sample chromatogram, on the position corresponding to control medicinal material chromatogram, and the principal spot of aobvious same color;
(2) TLC distinguish of smilax water extract gets this product in right amount, and porphyrize, takes 0.2g, adds 70% methyl alcohol 5ml, ultrasonic process 10 minutes, and filter, filtrate is as need testing solution.Separately fetch earth Poria cocos control medicinal material 0.3g, adds 70% methyl alcohol 2ml, ultrasonic process 20 minutes, gets supernatant medicinal material solution in contrast.According to thin-layered chromatography (annex V I B) test, draw above-mentioned control medicinal material solution and each 5 ~ 8 μ l of need testing solution, put respectively in same silica G F
254on thin layer plate, with volume ratio be the methenyl choloride-acetate-methanol-formic acid of 8: 2: 3: 0.3 for developping agent, launch, take out, hot blast drying, inspects under putting ultraviolet lamp 365nm, in test sample chromatogram, on the position corresponding to control medicinal material chromatogram, aobvious same color fluorescence principal spot; Inspect under putting ultraviolet lamp 254nm, in test sample chromatogram, on the position corresponding to control medicinal material chromatogram, aobvious same color principal spot; Spray with 10% ethanol solution of sulfuric acid again, 105 DEG C to be heated to spot development clear, in test sample chromatogram, on the position corresponding to control medicinal material chromatogram, and the principal spot of aobvious same color.
2. the quick multi information thin-layer identification method of a kind of rhizoma smilacis glabrae medicinal material according to claim 1 and water extract thereof, is characterized in that the described suitable primary crude drug 7 ~ 8g of the every 1g of radix scrophulariae water extract.
3. the quick multi information thin-layer identification method of a kind of rhizoma smilacis glabrae medicinal material according to claim 1 and water extract thereof, is characterized in that described water extract also can be the granule that its water extraction is got.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108535399A (en) * | 2018-04-09 | 2018-09-14 | 吉林省中研药业有限公司 | A kind of detection method of Fuyankang pill |
| CN111024879A (en) * | 2020-01-08 | 2020-04-17 | 河北中医学院 | Rapid multi-information thin-layer identification method for fresh motherwort herb freeze-dried powder, particles and standard decoction dry powder |
| CN112326862A (en) * | 2020-09-29 | 2021-02-05 | 鸿翔中药科技有限责任公司 | Poria cocos formula granules, preparation method thereof and thin-layer identification method |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08127532A (en) * | 1994-10-31 | 1996-05-21 | Takeshi Atai | Thermocataplasm |
| JPH08283172A (en) * | 1995-04-14 | 1996-10-29 | Kose Corp | Active oxygen scavenger and skin preparation for external use containing the scavenger |
| US20030152588A1 (en) * | 2002-01-14 | 2003-08-14 | Hsu-Shan Huang | Chinese traditional medicines for psoriasis |
| CN101317941A (en) * | 2007-06-08 | 2008-12-10 | 北京中医药大学 | Medicament for promoting diuresis and easing pain |
| CN101530506A (en) * | 2009-05-05 | 2009-09-16 | 杨文龙 | Method for analyzing poisonous clear tablet |
| CN102198210A (en) * | 2010-03-26 | 2011-09-28 | 云南良方制药有限公司 | Quality control method of xiaojiean preparation |
| CN103028065A (en) * | 2012-12-07 | 2013-04-10 | 贵州师范大学 | Preparation containing herba violae, rhizoma cyperi and herba leonuri and preparation method and detection method thereof |
-
2015
- 2015-07-23 CN CN201510437686.5A patent/CN104991031B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08127532A (en) * | 1994-10-31 | 1996-05-21 | Takeshi Atai | Thermocataplasm |
| JPH08283172A (en) * | 1995-04-14 | 1996-10-29 | Kose Corp | Active oxygen scavenger and skin preparation for external use containing the scavenger |
| US20030152588A1 (en) * | 2002-01-14 | 2003-08-14 | Hsu-Shan Huang | Chinese traditional medicines for psoriasis |
| CN101317941A (en) * | 2007-06-08 | 2008-12-10 | 北京中医药大学 | Medicament for promoting diuresis and easing pain |
| CN101530506A (en) * | 2009-05-05 | 2009-09-16 | 杨文龙 | Method for analyzing poisonous clear tablet |
| CN102198210A (en) * | 2010-03-26 | 2011-09-28 | 云南良方制药有限公司 | Quality control method of xiaojiean preparation |
| CN103028065A (en) * | 2012-12-07 | 2013-04-10 | 贵州师范大学 | Preparation containing herba violae, rhizoma cyperi and herba leonuri and preparation method and detection method thereof |
Non-Patent Citations (6)
| Title |
|---|
| FUMIYUKI KIUCHI 等: "Inhibition of Prostaglandin Biosynthesis by the Constituents of Medicinal Plants", 《CHEMICAL AND PHARMACEUTICAL BULLETIN》 * |
| ZHANG QING-FENG: "Antioxidant activity of Rhizoma Smilacis Glabrae extracts and its key constituent-astilbin", 《FOOD CHEMISTRY》 * |
| 代云桃 等: "复方青黛胶囊质量控制研究", 《中成药》 * |
| 张爱荣 等: "肾复康胶囊质量标准研究", 《山西医科大学学报》 * |
| 石小鹏 等: "参蛇软膏的质量标准研究", 《西北药学杂志》 * |
| 闫旭 等: "儿童排铅咀嚼片的质量标准研究", 《西部中医药》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108535399A (en) * | 2018-04-09 | 2018-09-14 | 吉林省中研药业有限公司 | A kind of detection method of Fuyankang pill |
| CN108535399B (en) * | 2018-04-09 | 2020-05-08 | 吉林省中研药业有限公司 | Detection method of Fuyankang pills |
| CN111024879A (en) * | 2020-01-08 | 2020-04-17 | 河北中医学院 | Rapid multi-information thin-layer identification method for fresh motherwort herb freeze-dried powder, particles and standard decoction dry powder |
| CN111024879B (en) * | 2020-01-08 | 2021-08-31 | 河北中医学院 | Rapid multi-information thin-layer identification method for fresh motherwort herb freeze-dried powder, particles and standard decoction dry powder |
| CN112326862A (en) * | 2020-09-29 | 2021-02-05 | 鸿翔中药科技有限责任公司 | Poria cocos formula granules, preparation method thereof and thin-layer identification method |
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| CN104991031B (en) | 2016-10-19 |
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