CN104698120B - The TLC Identification of the wind-weed in Antibicrobial anti inflammatory capsule - Google Patents
The TLC Identification of the wind-weed in Antibicrobial anti inflammatory capsule Download PDFInfo
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Abstract
The present invention relates to the TLC Identification of the wind-weed in Antibicrobial anti inflammatory capsule. The method, taking sarsasapogenin as reference substance, is used the one in following two kinds of solvent systems: cyclohexane-isopropyl alcohol (8.5-9.5: 0.5-1.5) or cyclohexane-isopropyl alcohol-strong ammonia solution, (8.5-9.5: 0.5-1.5: 0.2). The present invention can ensure, under the prerequisite of identification result, not use toxic agent, to testing crew and environmental friendliness with respect to prior art; Overcome the problem of launching the negative interference of reagent in existing Antibicrobial anti inflammatory capsule wind-weed thin-layer chromatography discriminating.
Description
Technical field
The present invention relates to the quality determining method of Antibicrobial anti inflammatory capsule, especially relate to the thin-layer chromatography discriminating side of the wind-weed in Antibicrobial anti inflammatory capsuleMethod.
Background technology
Antibicrobial anti inflammatory capsule is to record in the anti-inflammation sheet of the 7th of the drug standards promulgated by the ministries or commissions of the Central Government to change formulation kind, by honeysuckle, the tuber of stemona, HuangThe 7 taste Chinese medicines such as a kind of reed mentioned in ancient books, rheum officinale, folium isatidis, the wind-weed, desmodium form, and have effect of heat-clearing, purging intense heat, removing toxic substances. Former tablet standard is not rightIn prescription, any active ingredient is carried out qualitative, quantitative monitoring. In " Chinese pharmacopoeia " (version in 2010), do not take in relevant anti-inflammation glue yetThe method of capsule assay and standard. The wind-weed is the key component of Antibicrobial anti inflammatory capsule, for effectively controlling the quality of Antibicrobial anti inflammatory capsule, inspectionThe wind-weed of surveying is wherein necessary. And the main component of the wind-weed is timosaponin class, its aglycon is mainly sarsasapogenin, sarsasapogeninBe soluble in the low poles such as ethanol, benzinum and benzene to medium polar solvent. In prior art for the discriminating of the wind-weed or its preparation mainly with chinaroot greenbrierSapogenin is reference substance, taking benzinum and benzene as solvent or solvent. Have document taking benzene-acetone (9: 1) as solvent, but benzene there is playToxicity, harmful to experimenter and environment, and this solvent can make, and negative sample generating portion is negative to be disturbed. Should study be thus applicable to the wind-weed orThe non-toxicity solvent of its preparation.
Summary of the invention
The present invention, for overcoming above technological deficiency, provides the TLC Identification of the wind-weed in a kind of Antibicrobial anti inflammatory capsule, and the method makesWith non-toxic solvents and solvent, safe and effective.
During in Antibicrobial anti inflammatory capsule, the thin-layered chromatography of the wind-weed is differentiated, the present invention, taking sarsasapogenin as reference substance, uses following solventSystem:
(1) cyclohexane-isopropyl alcohol, proportioning is 8.5-9.5: 0.5-1.5 by test solution volume ratio;
(2) cyclohexane-isopropyl alcohol-strong ammonia solution, proportioning is 8.5-9.5: 0.5-1.5 by test solution volume ratio: 0.2.
Preferred solvent system is wherein:
A, cyclohexane-isopropyl alcohol, proportioning is 9: 1 by test solution volume ratio;
B, cyclohexane-isopropyl alcohol-strong ammonia solution, proportioning is 9.5: 0.5: 0.2 by test solution volume ratio;
C, cyclohexane-isopropyl alcohol-strong ammonia solution, proportioning is 9.5: 1: 0.2 by test solution volume ratio;
D, cyclohexane: isopropyl alcohol, proportioning is 9: 1 by test solution volume ratio, ammonia steam presaturation 20min.
Concrete discrimination method comprises the following steps:
1, the preparation of need testing solution: get Antibicrobial anti inflammatory capsule content, with alcohol heating reflux extraction, concentrated, concentrate hexamethyleneAlkane extracts, and makes by the cyclohexane solution of content 3g/ml;
2, reference substance solution preparation: get sarsasapogenin reference substance, add cyclohexane and make the solution of every 1ml containing 1mg, product in contrastSolution;
3, launch: by Chinese pharmacopoeia version in 2010, an annex VI B tests according to thin-layered chromatography, need testing solution and reference substance solutionPoint sample amount be 5 μ l, put respectively on same silica gel g thin-layer plate, launch with any solvent in above-mentioned solvent;
4, colour developing: spray, with the mixed solution of 8% vanillic aldehyde ethanol solution-sulfuric acid solution, is heated to spot colour developing at 100 DEG C clear,Inspect; In test sample chromatogram, with the corresponding position of reference substance chromatogram on, the spot of aobvious same color is qualified.
Wherein, preferably following methods of need testing solution preparation: get content 3g, add ethanol 20ml, add hot reflux 40 minutes, filter,Filtrate adds hydrochloric acid 1ml, adds hot reflux 1 hour, is concentrated into about 10ml, and the 10ml that adds water, with cyclohexane 20ml jolting extraction, extractEvaporate to dryness, residue adds cyclohexane 1ml to be made to dissolve, as need testing solution.
The present invention has successfully solved the thin-layer chromatography of the wind-weed in Antibicrobial anti inflammatory capsule and has differentiated, particularly effectively control in production or Medicine inspectionThe problem of Antibicrobial anti inflammatory capsule quality processed. The method, with respect to existing discrimination method, is ensureing, under the prerequisite of identification result, not use poisonProperty reagent, to testing crew and environmental friendliness; And overcome the negative problem of disturbing of existing expansion reagent.
Brief description of the drawings
Fig. 1 is solvent system: cyclohexane: the chromatogram of isopropyl alcohol (9: 1).
Fig. 2 is solvent system: cyclohexane: isopropyl alcohol: the chromatogram of strong ammonia solution (9.5: 0.5: 0.2).
Fig. 3 is solvent system: cyclohexane: isopropyl alcohol: the chromatogram of strong ammonia solution (9.5: 1: 0.2).
Fig. 4 is solvent system cyclohexane: isopropyl alcohol (9: 1), the chromatogram of ammonia steam presaturation 20min.
Fig. 5 is the inspection collection of illustrative plates of 10 batch samples.
Detailed description of the invention
The chromatographic condition of the tlc identification method of wind-weed research in Antibicrobial anti inflammatory capsule.
1 instrument and reagent for test
ME203E type electronic balance [Mettler-Toledo Instrument (Shanghai) Co., Ltd.]; Silica G thin-layer chromatography prefabricated board (Qing DaohaiOcean Chemical Co., Ltd.); Silica G thin-layer chromatography prefabricated board (the biochemical plastic molding and processing plant of Taizhou City of Zhejiang Province road and bridge tetramethyl); Silica G thin-layer chromatography is pre-Making sheet (Merck & Co., Inc.); Silica G for thin-layer chromatography (Qingdao Marine Chemical Co., Ltd.); Antibicrobial anti inflammatory capsule (Hang Seng of Zhongshan city medicine company20130305,20131004,20131201,20140201,20140301,20140401,20140405 Co., Ltd, lot number:,20140506,20140701,20140702); The negative preparation of the Antibicrobial anti inflammatory capsule wind-weed (Hengsheng Pharmaceutical Co., Ltd., Zhongshan City, lot number:DP20131101); Sarsasapogenin reference substance (Nat'l Pharmaceutical & Biological Products Control Institute, lot number: 110744-201310); Water is purified water;It is pure that cyclohexane, normal heptane, isopropyl alcohol, acetone, ethyl acetate, formic acid, benzinum (60～90 DEG C), chloroform are analysis.
2 methods and result
2.1 analytical methods:
Get Antibicrobial anti inflammatory capsule content 3g, add ethanol 20ml, add hot reflux 40 minutes, filter, filtrate adds hydrochloric acid 1ml, heats backFlow 1 hour, be concentrated into about 10ml, the 10ml that adds water, with cyclohexane 20ml jolting extraction, extract evaporate to dryness, residue adds cyclohexane 1mlMake to dissolve, as need testing solution. Separately get sarsasapogenin reference substance, add cyclohexane and make the solution of every 1mL containing 1mg, in contrastProduct solution. According to thin-layered chromatography (annex IV B of Chinese pharmacopoeia version in 2010) test, draw Antibicrobial anti inflammatory capsule need testing solution 4 μ lAnd sarsasapogenin reference substance solution 5 μ l, put respectively on same silica gel g thin-layer plate, with solvent, launch, take out, dry sprayWith the mixed liquor (0.5: 5) of 8% vanillic aldehyde ethanol solution and sulfuric acid solution (7 → 10), be heated to spot colour developing at 100 DEG C clear.In test sample chromatogram, with the corresponding position of reference substance chromatogram on, the spot of aobvious same color.
2.1.1 the preparation of need testing solution
Get this product content 3g, add ethanol 20ml, add hot reflux 40 minutes, filter, filtrate adds hydrochloric acid 1ml, adds hot reflux 1 hour,Be concentrated into about 10ml, the 10ml that adds water, with cyclohexane 20ml jolting extraction, extract evaporate to dryness, residue adds cyclohexane 1ml to be made to dissolve, and doesFor need testing solution.
2.1.2 the preparation of reference substance solution
Get sarsasapogenin reference substance, add cyclohexane and make the solution of every 1ml containing 1mg, product solution in contrast.
2.1.3 the preparation of negative control solution
Get the negative preparation 3g of the Antibicrobial anti inflammatory capsule wind-weed, prepare by need testing solution preparation method.
2.2 the research of chromatographic condition
2.2.1 fix phase, colour developing and inspection method
The fixing silica gel g thin-layer plate that adopts mutually; Colour developing and inspection method are for spray is with 8% vanillic aldehyde ethanol solution-[sulfuric acid solution (7 → 10)]The mixed solution of (1: 10), is heated to spot colour developing at 100 DEG C clear, under daylight, inspects.
2.2.2 solvent
Use respectively following seven kinds of different solvent systems, investigate with different solvent ratios, and attempt adding in solvent a small amount ofFormic acid, strong ammonia solution or put in the pre-saturated expansion cylinder of ammonia steam and launch to improve spot, the expansion effect of more different solvent systems.
(1) cyclohexane-acetone system, investigate respectively following solvent:
1. cyclohexane: acetone (9: 1);
2. cyclohexane: acetone (7: 1;
3. cyclohexane: acetone (5: 1);
4. cyclohexane: acetone: formic acid (7: 1: 0.1).
Result: by the comparative analysis of gained thin-layer chromatography, adopt the development system of cyclohexane-acetone aforementioned proportion, in each test sample chromatogram,With all spots of aobvious same color of the corresponding position of reference substance chromatogram, but spot is clear not; And negative control chromatogram and reference substance chromatogramCorresponding positions is equipped with other lighter color spot, easily causes interference.
(2) cyclohexane-ethyl acetate system, investigate respectively following solvent:
1. cyclohexane: ethyl acetate (7: 3);
2. cyclohexane: ethyl acetate: formic acid (7: 3: 0.1);
3. cyclohexane: ethyl acetate: formic acid (4: 1: 0.1).
Result, from the comparative analysis of gained thin-layer chromatography, adopts in the development system of cyclohexane-ethyl acetate aforementioned proportion,In test sample chromatogram, with all spots of aobvious same color of the corresponding position of reference substance chromatogram, but negative control chromatogram shows other in relevant positionColor spot, causes obvious interference.
(3) normal heptane-acetone system, investigate respectively following solvent:
1. normal heptane: acetone (4: 1);
2. normal heptane: acetone: formic acid (8: 2: 0.1).
Result: adopt normal heptane: the development system of acetone aforementioned proportion, in each test sample chromatogram, with reference substance chromatogramOn corresponding position, all show the spot of same color, but negative control there is obvious interference.
(4) normal heptane-ethyl acetate system, investigate respectively following solvent:
1. normal heptane: ethyl acetate (2: 1);
2. normal heptane: ethyl acetate (3: 1);
3. normal heptane: ethyl acetate (7: 3).
Result: adopt normal heptane: the development system of ethyl acetate aforementioned proportion, in each test sample chromatogram, with reference substance lookCompose all spots of aobvious same color of corresponding position, but negative control there is obvious interference.
(5) normal heptane-isopropyl alcohol system, investigate respectively following solvent:
1. normal heptane: isopropyl alcohol (4: 1);
2. normal heptane: isopropyl alcohol (9: 1);
3. normal heptane: isopropyl alcohol: strong ammonia solution (9: 1: 0.2).
Result: from chromatogram, adopt normal heptane: the development system of isopropyl alcohol aforementioned proportion, in each test sample chromatogram, with contrastThe corresponding position of product chromatogram is the spot of aobvious same color all, and negative control is noiseless, add strong ammonia solution after spot shape be improved. ButEach spot point separating degree is bad.
(6) cyclohexane-isopropyl alcohol system, investigate respectively following solvent:
A. cyclohexane-isopropyl alcohol, proportioning is 7: 0.3 by test solution volume ratio;
B. cyclohexane-isopropyl alcohol, proportioning is 8: 1 by test solution volume ratio;
C. cyclohexane-isopropyl alcohol, proportioning is 8.5: 0.5 by test solution volume ratio;
D. cyclohexane-isopropyl alcohol, proportioning is 9: 1 by test solution volume ratio;
E. cyclohexane-isopropyl alcohol, proportioning is 9.5: 1.5 by test solution volume ratio;
F. cyclohexane-isopropyl alcohol, proportioning is 9.5: 1 by test solution volume ratio;
G. cyclohexane-isopropyl alcohol-strong ammonia solution, proportioning is 8: 0.5: 0.1 by test solution volume ratio;
H. cyclohexane-isopropyl alcohol-strong ammonia solution, proportioning is 8.5: 1.5: 0.2 by test solution volume ratio;
I. cyclohexane-isopropyl alcohol-strong ammonia solution, proportioning is 9: 1: 0.2 by test solution volume ratio;
J. cyclohexane-isopropyl alcohol-strong ammonia solution, proportioning is 9.5: 0.5: 0.2 by test solution volume ratio;
K. cyclohexane-isopropyl alcohol-strong ammonia solution, proportioning is 9.5: 1: 0.2 by test solution volume ratio;
L. cyclohexane-isopropyl alcohol-strong ammonia solution, proportioning is 10: 1: 0.1 by test solution volume ratio;
M. cyclohexane-isopropyl alcohol-strong ammonia solution, proportioning is 10: 1.5: 0.2 by test solution volume ratio;
N, cyclohexane-isopropyl alcohol, proportioning is 9: 1 by test solution volume ratio, ammonia steam presaturation 20min.
More than testing sample sequence number 1 in chromatogram is wind-weed negative control, and 2 is Antibicrobial anti inflammatory capsule, and 3 is Antibicrobial anti inflammatory capsule, and 4 areAntibicrobial anti inflammatory capsule, 5 is sarsasapogenin reference substance. The sample one that in solvent screening chromatogram of the present invention, the chromatogram of same sequence number is usedCause.
Result: from the chromatogram contrast of gained, except A, B, G, L and M group, in other test sample chromatograms of respectively organizing, withThe corresponding position of reference substance chromatogram is the spot of aobvious same color all, and negative control is noiseless. Especially with the separating degree of following several groups of spotsBest:
1. cyclohexane: isopropyl alcohol (9: 1); Gained chromatogram is shown in Fig. 1.
2. cyclohexane: isopropyl alcohol: strong ammonia solution (9.5: 0.5: 0.2); Gained chromatogram is shown in Fig. 2.
3. cyclohexane: isopropyl alcohol: strong ammonia solution (9.5: 1: 0.2); Gained chromatogram is shown in Fig. 3.
4. cyclohexane: isopropyl alcohol (9: 1), ammonia steam presaturation 20min. Gained chromatogram is shown in Fig. 4.
Therefore, determine with cyclohexane-isopropyl alcohol (8.5-9.5: 0.5-1.5) and cyclohexane-isopropyl alcohol-strong ammonia solution(8.5-9.5: 0.5-1.5: 0.2) as solvent.
(7) other development system: in research process, researcher once investigated " toluene: acetone (9: 1), toluene: acetone:Formic acid (9: 1: 0.2), toluene: isopropyl alcohol (9: 0.5), benzinum (60～90 DEG C): acetone (7: 1), chloroform: acetone(7: 2) " etc. other development system, but effect is all not ideal enough.
The investigation conclusion of solvent: the to sum up investigation result of several solvent systems, with cyclohexane: isopropyl alcohol: strong ammonia solution (9.5:0.5: 0.2) for solvent launch chromatogram in, the separating degree of each spot is good, in test sample chromatogram with the corresponding position of reference substance chromatogramThe spot of upper aobvious same color, negative control is noiseless, and method is easy and simple to handle. Therefore tentatively selected this solvent.
2.3 methodological study
2.3.1 specificity
Test method: draw Antibicrobial anti inflammatory capsule need testing solution, sarsasapogenin reference substance solution and Antibicrobial anti inflammatory capsule wind-weed feminine genderContrast solution, puts respectively on same silica gel g thin-layer plate, taking cyclohexane: isopropyl alcohol: strong ammonia solution (9.5: 0.5: 0.2) is as launchingAgent, launches, and takes out, and dries, and sprays the mixed solution with 8% vanillic aldehyde ethanol solution-[sulfuric acid solution (7 → 10)] (1: 10),Be heated to spot colour developing at 100 DEG C clear, under daylight, inspect.
Result of the test: in Antibicrobial anti inflammatory capsule test sample chromatogram, with the corresponding position of sarsasapogenin reference substance chromatogram on, aobvious identicalThe spot of color, and in wind-weed negative control chromatogram, noiselessly on relevant position shows that this method has specially the discriminating of sarsasapogeninAttribute.
2.3.2 durability
(1) different lamellaes
Test method: use respectively following different types of lamellae to test, investigate according to the method under 2.3.1 item.
1. silica G self-control hand bed board (glass): 10cm × 10cm, thickness 0.5mm.
2. silica G thin-layer chromatography prefabricated board (aluminium foil): 10cm × 10cm, thickness 0.25mm, the biochemical plastics of Taizhou City of Zhejiang Province road and bridge tetramethylFactory.
3. silica G thin-layer chromatography prefabricated board (glass): 10cm × 10cm, thickness 0.25mm, Qingdao Marine Chemical Co., Ltd..
4. silica G thin-layer chromatography prefabricated board (aluminium foil): 10cm × 10cm, thickness 0.25mm, Merck & Co., Inc..
Result of the test: from gained chromatogram, adopt the lamellae of above different manufacturers, unlike material to carry out chromatography, each sample chromatogramSpot all can effectively separate, and shows that this method has durability to the variation of lamellae type. Therefore, in text, do not specify to use which kind of classThe lamellae of type.
Glass plate duration of run is longer, needs 50min, and aluminum foil plate only needs 25min. Therefore following investigation is all used aluminum foil plate to carry out.
(2) point sample mode
Test method: carry out point sample by round point shape, two kinds of point sample modes of ribbon respectively, investigate according to the method under 2.3.1 item.
Result of the test shows: adopt round point shape and ribbon point sample mode, the spot of each sample chromatogram all can effectively separate, and shows this methodDifference sample loading mode is had to durability, therefore, in text, do not specify to use which kind of point sample mode.
(3) point sample amount
Test method: according to the method under 2.3.1 item, need testing solution is got respectively 2 μ l, 4 μ l, 5 μ l, 10 μ l, reference substance solutionAnd negative control solution gets 5 μ l by primary standard specified content and carry out point sample, investigate.
Result of the test: in the test sample chromatogram of above-mentioned 4 kinds of different point sample amounts, with equal aobvious identical face on the corresponding position of reference substance chromatogramThe spot of look, shows that this method has durability to the little variation of point sample amount. And from each chromatogram, need testing solution point sample amount be 2 μ l,When 4 μ l, the spot colors of test sample chromatogram and reference substance chromatogram relevant position is lighter, and when point sample amount is 10 μ l, volume containing the sample is excessive,Spot has diffusion. And point sample amount is while being 5 μ l, test sample chromatogram and the corresponding spot shape of reference substance chromatogram, sizableness. Therefore, intendThe point sample amount of determining need testing solution is 5 μ l.
(4) different temperatures
Test method: according to the method under 2.3.1 item, launch under normal temperature (10～30 DEG C) and low temperature (4～10 DEG C) condition respectively, enterRow is investigated.
Result of the test: launch each spot and all can effectively separate under normal temperature and low temperature. Compare cryogenic conditions, the spot under normal temperature condition separatesDegree better.
The inspection of (5) 10 batches of products
Test method: get respectively the Antibicrobial anti inflammatory capsule product in 10 batches of different production periods, test according to the method under 2.3.1 item.
Result of the test (referring to Fig. 5): in test sample chromatogram, all feature spots of aobvious sarsasapogenin reference substance, and negative noiseless.
In Fig. 5,1 is wind-weed negative control product, and 2-11 is respectively 10 batches of Antibicrobial anti inflammatory capsule samples, and 12 is sarsasapogenin reference substance.
The comprehensively result of above-mentioned " research of chromatographic condition " and " methodological study ", the sarsasapogenin mirror in the Antibicrobial anti inflammatory capsule wind-weedOther method is specially: get this product content 3g, add ethanol 20ml, add hot reflux 40 minutes, filter, filtrate adds hydrochloric acid 1ml, heatingReflux 1 hour, be concentrated into about 10ml, the 10ml that adds water, with cyclohexane 20ml jolting extraction, extract evaporate to dryness, residue adds cyclohexane 1mlMake to dissolve, as need testing solution. Separately get sarsasapogenin reference substance, add cyclohexane and make the solution of every 1ml containing 1mg, in contrastProduct solution. According to thin-layered chromatography (annex VI B of Chinese pharmacopoeia version in 2010) test, draw the each 5 μ l of above-mentioned two kinds of solution, respectivelyPoint, on same silica gel g thin-layer plate, taking cyclohexane-isopropyl alcohol-strong ammonia solution (9.5: 0.5: 0.2) as solvent, launches, takes out,Dry, spray, with the mixed solution of 8% vanillic aldehyde ethanol solution-[sulfuric acid solution (7 → 10)] (1: 10), is heated to spot at 100 DEG CPoint colour developing is clear, under daylight, inspects. In test sample chromatogram, with the corresponding position of reference substance chromatogram on, the spot of aobvious same color.
Claims (4)
1. the TLC Identification of the wind-weed in Antibicrobial anti inflammatory capsule, comprises need testing solution preparation, reference substance solution systemStandby, expansion and development step, taking sarsasapogenin as reference substance; It is characterized in that:
The solvent of described method is: cyclohexane-isopropyl alcohol, and proportioning is 8.5-9.5: 0.5-1.5 by test solution volume ratio; Or
Cyclohexane-isopropyl alcohol-strong ammonia solution, proportioning is 8.5-9.5: 0.5-1.5 by test solution volume ratio: 0.2;
Described need testing solution preparation process is: get Antibicrobial anti inflammatory capsule content, and with alcohol heating reflux extraction, concentrated,Concentrate extracts with cyclohexane, makes by the cyclohexane solution of content 3g/ml;
Described reference substance solution preparation process is: get sarsasapogenin reference substance, add cyclohexane and make molten containing 1mg of every 1mlLiquid, in contrast product solution.
2. the method for claim 1, is characterized in that, described solvent is:
(1) cyclohexane-isopropyl alcohol, proportioning is 9: 1 by test solution volume ratio; Or
(2) cyclohexane-isopropyl alcohol-strong ammonia solution, proportioning is 9.5: 0.5: 0.2 by test solution volume ratio; Or
(3) cyclohexane-isopropyl alcohol-strong ammonia solution, proportioning is 9.5: 1: 0.2 by test solution volume ratio; Or
(4) cyclohexane: isopropyl alcohol, proportioning is 9: 1 by test solution volume ratio, ammonia steam presaturation 20min.
3. method as claimed in claim 1 or 2, is characterized in that: described need testing solution preparation process is; In gettingTolerant 3g, adds ethanol 20ml, adds hot reflux 40 minutes, filters, and filtrate adds hydrochloric acid 1ml, adds hot reflux 1 hour, concentratedTo about 10ml, the 10ml that adds water, with cyclohexane 20ml jolting extraction, extract evaporate to dryness, residue adds cyclohexane 1ml to be made to dissolve,As need testing solution.
4. method as claimed in claim 1 or 2, is characterized in that:
Described deployment step is: by Chinese pharmacopoeia version in 2010, an annex VI B tests according to thin-layered chromatography, test sampleThe point sample amount of solution and reference substance solution is 5 μ l, puts respectively on same silica gel g thin-layer plate;
Described development step is: spray, with the mixed solution of 8% vanillic aldehyde ethanol solution-sulfuric acid solution, adds at 100 DEG CHeat is clear to spot colour developing, inspects; In test sample chromatogram, with the corresponding position of reference substance chromatogram on, the spot of aobvious same colorPoint.
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