CN1673351A - Short lactobacillus producing gamma-aminobutyric acid and use thereof - Google Patents
Short lactobacillus producing gamma-aminobutyric acid and use thereof Download PDFInfo
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- CN1673351A CN1673351A CN 200510049188 CN200510049188A CN1673351A CN 1673351 A CN1673351 A CN 1673351A CN 200510049188 CN200510049188 CN 200510049188 CN 200510049188 A CN200510049188 A CN 200510049188A CN 1673351 A CN1673351 A CN 1673351A
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- aminobutyric acid
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Abstract
The present invention discloses one kind of gamma-aminobutyric acid producing lactobacillus brevis and its use. The lactobacillus brevis has preservatioin number of CGMCC No. 1306; exhibits pairs or chains; and has no motion, no spore, small colony and smooth surface. It belongs to Gram positive bacteria and facultative anaerobe, and may be used in producing gamma-aminobutyric acid. By means of biological synthesis process, the high activity glutamate decarboxylase connected in the lactobacillus brevis and sodium L-glutamate as substrate, the sodium L-glutamate is decarboxylated to produce gamma-aminobutyric acid and CO2. Thus produced gamma-aminobutyric acid is safe in food level.
Description
Technical field
The present invention relates to a kind of short lactobacillus that produces γ-An Jidingsuan and uses thereof.
Background technology
Up to now, the mankind have had been found that nearly tens kinds of neurotransmitters.After deliberation the proof, some amino acids mediators be one of factor of bringing out multiple nervous system disorders (as epilepsy, Parkinson's disease) unusually.γ-An Jidingsuan (being called for short GABA) claims aminobutyric acid again, is a kind of important nonprotein amino acid, as the important neurotransmitter of central nervous system, has the important physical function.The lot of documents report, GABA can be hypotensive and cholesterol, be prevented that neurocyte is overheated, thereby have and calm the nerves and resist the depressed effect that removes, and can also cerebral function improvement and long-term memory, increase growth hormone secretion, prevent obesity, the sharp kidney of strong liver, improve function such as climacteric syndrome.In May, 2003, internal authority academic journals " science " magazine has been published the Zhou Yifeng of Chinese University of Science and Technology and has been taught the current research of cooperating to finish with U.S. scientist.Studies show that the aging of people's brain might cause because its brain can't obtain the chemicals γ-An Jidingsuan (GABA) of q.s.Along with the research that deepens continuously to the GABA physiologically active, GABA also has been subjected to more concern more and more." integration of drinking and medicinal herbs " is a kind of methods of treatment of advocating at present, seeks and develop the focus that pure natural medical has become research.Therefore, GABA both can develop becomes a kind of food that had both had health, can be developed to the medicine with remarkable pharmacological action again.In recent years, utilize Rice plumule, development of raw materials such as tealeaves produce the health foodstuff series batching that is rich in GABA.With tea drinks in Japan Gabaron is representative, confirms that through animal experiment and clinical trial high gamma-aminobutyric acid tea has tangible blood pressure lowering effect, does not have any physically different phenomenon.Recently, Japanese PCI Company utilizes the fermentative Production γ-An Jidingsuan, as functional food ingredient, has been widely used in goods such as beverage, jam, cake, biscuit, food flavouring.In China, also there is bibliographical information to detect GABA at genseng, state guest's tea, mulberry tea, Chinese medicine astragalus etc.Researcher professor Huang Danian of China Paddy Rice Inst utilizes the biotechnology breeding method to cultivate function rice new variety, contains abundant γ-An Jidingsuan, and it is remarkable to be used for the aided blood pressure-lowering effect, and total effective rate is 82.9%.The domestic demand of γ-An Jidingsuan in recent years market growth is quite fast, and the pharmaceutical factory that the aminobutyric acid sheet is produced in State Food and Drug Administration's approval has reached 24.At present, GABA is produced in domestic main employing chemosynthesis, and the research of the biosynthesizing γ-An Jidingsuan of employing new technology remains to be developed energetically very little.
Compare with the chemical synthesis process that GABA is traditional, the synthetic GABA of biological process has many outstanding advantages, and major advantage is the synthesis condition gentleness, and environmental pollution is little, and energy consumption is little.One of key that adopts the synthetic GABA of biological process is exactly to filter out the strain excellent with efficient synthetic GABA.The biosynthesizing of past GABA adopts intestinal bacteria as bacterial strain usually, but has the hidden danger of safety and sanitation aspect as the production bacterial strain with intestinal bacteria.Bibliographical information in recent years, the microorganism of some aliment security levels such as milk-acid bacteria, monascus also have the ability of synthetic GABA.This paper has reported that a strain has the short lactobacillus of the synthetic GABA ability of high-performance bio.
Summary of the invention
The purpose of this invention is to provide a kind of short lactobacillus that produces γ-An Jidingsuan and uses thereof.
Short lactobacillus (Lactobacillus brevis), its deposit number is: CGMCC NO.1306, microscopic examination is paired or become chain to occur, do not move, no spore, bacterium colony is little, smooth surface; Belong to gram positive bacterial strain, amphimicrobian.
Deposit number is: the short lactobacillus of CGMCC NO.1306 (Lactobacillus brevis) is used to produce γ-An Jidingsuan.
The operational condition that this bacterial strain is used to produce γ-An Jidingsuan is: bacterial strain is after the agar slant culture-medium activation, transfer in GYP seed culture medium or MRS seed culture medium, cultivate after 10～30 hours, inoculum size inoculation GYP or MRS fermention medium with 0.5%～5%, under 25 ℃～35 ℃, leave standstill and cultivate 48h～120h, promptly get the fermented liquid that contains γ-An Jidingsuan.
It is to utilize the lactic acid thalline to contain the high reactivity L-Glutamic decarboxylase that the present invention adopts the synthetic γ-An Jidingsuan of biological process, and this enzyme is a substrate with L-Sodium Glutamate (L-MSG), with L-Sodium Glutamate (L-MSG) α-decarboxylation, produces γ-An Jidingsuan and CO
2The health efficacy common people of lactobacillus product generally acknowledge, are thought GRAS (generally recognized as safe) level foodstuff additive by FDA.Therefore, there is not the hidden danger on the safety in the γ-An Jidingsuan that this method is produced, can reach aliment security level.
Embodiment
A. bacterial strain
This laboratory screening.
B. substratum
1000mL agar slant storage medium: glucose 10g, yeast extract paste 10g, peptone 5g, sodium acetate 2g, MgSO
47H
2O 20mg, MnSO
44H
2O 1mg, NaCl 1mg, FeSO
47H
2O 1mg, agar 15g, pH6.8.
1000mL selective medium: Trypicase 10g, yeast extract 5g, glucose 20g, dibasic ammonium citrate 2g, tween-80 1mL, CH
3COONa3H
2O 25g, KH
2PO
46g, MgSO
47H
2O0.58g, MnSO
44H
2O 0.15g, FeSO
47H
2O 0.03g, actidione 10mg, pH6.6-7.0, hour, seed culture fluid is inoculated in the MRS fermention medium with 1% inoculum size, add a hydration L-Sodium Glutamate of 1% in the fermention medium, the liquid amount of fermention medium is 200mL/500mL, leaves standstill to cultivate 72 hours, obtains gamma-aminobutyric acid fermentation, get the about 2g/L of content through efficient liquid phase chromatographic analysis.
Embodiment 3
Bacterial strain is after the agar slant culture-medium activation, transfer in the MRS seed culture medium, incubation time is 20 hours, seed culture fluid is inoculated in the GYP fermention medium with 1% inoculum size, adds a hydration L-Sodium Glutamate of 1% in the fermention medium, the liquid amount of fermention medium is 200mL/500mL, leave standstill and cultivated 60 hours, obtain gamma-aminobutyric acid fermentation, get, the about 2g/L of content through efficient liquid phase chromatographic analysis.
Embodiment 4
Bacterial strain is after the agar slant culture-medium activation, transfer in the MRS seed culture medium, incubation time is 36 hours, seed culture fluid is inoculated in the MRS fermention medium with 1% inoculum size, adds a hydration L-Sodium Glutamate of 1% in the fermention medium, the liquid amount of fermention medium is 200mL/500mL, leave standstill and cultivated 60 hours, obtain gamma-aminobutyric acid fermentation, get, the about 2g/L of content through efficient liquid phase chromatographic analysis.
Embodiment 5
Bacterial strain is after the agar slant culture-medium activation, transfer in the GYP seed culture medium, incubation time is 36 hours, seed culture fluid is inoculated in the GYP fermention medium with 2% inoculum size, adds a hydration L-Sodium Glutamate (L-MSG) of 1% in the fermention medium, the liquid amount of fermention medium is 200mL/500mL, leave standstill and cultivated 60 hours, obtain gamma-aminobutyric acid fermentation, get, the about 4g/L of content through efficient liquid phase chromatographic analysis.
Claims (3)
1. a short lactobacillus that produces γ-An Jidingsuan is characterized in that, short lactobacillus (Lactobacillus brevis), and its deposit number is: CGMCC NO.1306, microscopic examination is paired or become chain to occur, do not move, no spore, bacterium colony is little, smooth surface; Belong to gram positive bacterial strain, amphimicrobian.
2. the purposes of the short lactobacillus of a product γ-An Jidingsuan as claimed in claim 1 is characterized in that, deposit number is: the short lactobacillus of CGMCC NO.1306 (Lactobacillus brevis) is used to produce γ-An Jidingsuan.
3. a kind of purposes of producing the short lactobacillus of γ-An Jidingsuan according to claim 2, the operational condition that is used to produce γ-An Jidingsuan is: short lactobacillus is after the agar slant culture-medium activation, transfer in GYP seed culture medium or MRS seed culture medium, cultivate after 20～30 hours, inoculum size inoculation GYP or MRS fermention medium with 0.5%～5%, under 25 ℃～35 ℃, leave standstill and cultivate 48h～120h, promptly get the fermented liquid that contains γ-An Jidingsuan.
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| CN 200510049188 CN1276077C (en) | 2005-03-07 | 2005-03-07 | Short lactobacillus producing gamma-aminobutyric acid and use thereof |
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| CN 200510049188 CN1276077C (en) | 2005-03-07 | 2005-03-07 | Short lactobacillus producing gamma-aminobutyric acid and use thereof |
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| CN1673351A true CN1673351A (en) | 2005-09-28 |
| CN1276077C CN1276077C (en) | 2006-09-20 |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101144064B (en) * | 2007-09-03 | 2011-06-08 | 江南大学 | Lactobacillus brevis with anti-mutagenesis active and capable of producing exopolysaccharide and use thereof |
| CN101389751B (en) * | 2006-02-21 | 2011-09-07 | 龟甲万株式会社 | Lactic acid bacterium capable of producing gamma-aminobutyric acid |
| CN101724587B (en) * | 2009-09-10 | 2011-10-12 | 浙江师范大学 | Lactobacillus brevis L2 bacterial strain of high yield gamma-aminobutyrique and screening method and applications thereof |
| CN101935618B (en) * | 2009-05-07 | 2013-03-20 | 天津工业生物技术研究与发展中心 | Lactobacillus and method for producing lactic acid by fermentation of diluted acid hydrolyzate of lignocelluloses |
| CN103305561A (en) * | 2012-03-15 | 2013-09-18 | 江苏纳克生物工程有限公司 | Method for producing gama-aminobutyric acid by utilizing microbiological fermentation method |
| CN107118885A (en) * | 2017-05-17 | 2017-09-01 | 中国食品发酵工业研究院 | A kind of method that the fermented wine containing GABA is produced using the piece of resistance to ethanol coccus |
| CN107418912A (en) * | 2017-06-20 | 2017-12-01 | 江南大学 | One plant of Lactobacillus brevis for improving old face steamed bun ferment local-flavor and its application |
| CN109777748A (en) * | 2018-11-27 | 2019-05-21 | 南昌大学 | The Lactobacillus brevis CD0817 of one plant of highly producing gamma-aminobutyric acid |
| CN112300955A (en) * | 2019-07-29 | 2021-02-02 | 台湾中油股份有限公司 | Gamma-aminobutyric acid high-yield lactic acid bacterial strain and application thereof |
| CN113061061A (en) * | 2021-02-19 | 2021-07-02 | 安徽丰乐农化有限责任公司 | Preparation method of chemical fertilizer rich in gamma-aminobutyric acid |
| CN115369051A (en) * | 2022-04-24 | 2022-11-22 | 浙江省农业科学院 | Lactobacillus brevis for high yield of gamma-aminobutyric acid and application thereof |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101948767A (en) * | 2010-08-18 | 2011-01-19 | 南京农业大学 | Method for screening lactic acid bacteria producing gamma-amino butyric acid |
| CN102676598A (en) * | 2012-05-30 | 2012-09-19 | 广东乐尔康生物科技股份有限公司 | Method for catalytically synthesizing gamma-aminobutyric acid by using sodium glutamate and immobilized bio-enzyme |
| TWI669392B (en) * | 2017-12-20 | 2019-08-21 | 國立高雄科技大學 | Novel lactobacillus brevis and the application thereof |
-
2005
- 2005-03-07 CN CN 200510049188 patent/CN1276077C/en not_active Expired - Fee Related
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101389751B (en) * | 2006-02-21 | 2011-09-07 | 龟甲万株式会社 | Lactic acid bacterium capable of producing gamma-aminobutyric acid |
| CN101144064B (en) * | 2007-09-03 | 2011-06-08 | 江南大学 | Lactobacillus brevis with anti-mutagenesis active and capable of producing exopolysaccharide and use thereof |
| CN101935618B (en) * | 2009-05-07 | 2013-03-20 | 天津工业生物技术研究与发展中心 | Lactobacillus and method for producing lactic acid by fermentation of diluted acid hydrolyzate of lignocelluloses |
| CN101724587B (en) * | 2009-09-10 | 2011-10-12 | 浙江师范大学 | Lactobacillus brevis L2 bacterial strain of high yield gamma-aminobutyrique and screening method and applications thereof |
| CN103305561A (en) * | 2012-03-15 | 2013-09-18 | 江苏纳克生物工程有限公司 | Method for producing gama-aminobutyric acid by utilizing microbiological fermentation method |
| CN107118885A (en) * | 2017-05-17 | 2017-09-01 | 中国食品发酵工业研究院 | A kind of method that the fermented wine containing GABA is produced using the piece of resistance to ethanol coccus |
| CN107418912A (en) * | 2017-06-20 | 2017-12-01 | 江南大学 | One plant of Lactobacillus brevis for improving old face steamed bun ferment local-flavor and its application |
| CN107418912B (en) * | 2017-06-20 | 2019-09-17 | 江南大学 | One plant of Lactobacillus brevis for improving old face steamed bun ferment local-flavor and its application |
| CN109777748A (en) * | 2018-11-27 | 2019-05-21 | 南昌大学 | The Lactobacillus brevis CD0817 of one plant of highly producing gamma-aminobutyric acid |
| CN112300955A (en) * | 2019-07-29 | 2021-02-02 | 台湾中油股份有限公司 | Gamma-aminobutyric acid high-yield lactic acid bacterial strain and application thereof |
| CN113061061A (en) * | 2021-02-19 | 2021-07-02 | 安徽丰乐农化有限责任公司 | Preparation method of chemical fertilizer rich in gamma-aminobutyric acid |
| CN115369051A (en) * | 2022-04-24 | 2022-11-22 | 浙江省农业科学院 | Lactobacillus brevis for high yield of gamma-aminobutyric acid and application thereof |
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| CN1276077C (en) | 2006-09-20 |
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